Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0016904

Physical Location & Seq

Genomic Coordinates Forward (+)

5567779 .. 5569562

Gene Structure 1784 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1079300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1353 bp

ATGCCAGCTTCCAACTGCAGTTATGGTCTGAGAGAGCTGCTTGGGAAAGTATTGAAGAGGAATACAAAAAGAATTGATCAGTCTTCTGGTAATGAGATTACTGAGATGGAAAGCGTTACAAAAAAAGATAGAATTAGCAATCTGCCTTGGGATGTATTGGACGGGATCCTCGTTCGCTTGCCTTTGAAAGAAGTTGTGAGGACTAGCATCTTATCTAGCAGGTGGAGACATAAATGGACTGGCATTTCACAGTTTGTTATTGATGACAAATGCATACCACGCCGGATATCAGACAAAGTCGCAAGATGGGAATCGATCATGGAAATTCTTCATCAAGTTCAATTGCATCACACCGGTCCTATAGAAAAGTTTAAGCTCGCTGCTTATTGCCGGCCAGACCATTCTGATTTAGACCAGTGGATTCATTATTTAACTGACAAAGGTCTCAAGGAGTTCATCCTGCAGGAGTTTGATACTATAAAACGTTTTAACTTGCCTTTTTGTCTATTCTCATGTCCACTTTTGAATCGCTTGGAGCTTTTCGGTTGTAGAATCAAGCCATCTTCTGAAGCTATCGGATTCAAGAGCCTTGCGAACCTCCATCTTAATGAAGTTTGTGTAACTGGTGGCATGCTAGAATGTTTGGTTCTAAACTCCCCGGTTCTTGAAAGATTGACACTGTTAAACATTGATCATCAAATTGTTCTTAGAATTGGCAACGCGAATCTCAAGTATCTGAAAGTAGATTCCAACTTTGGCGACATATATCTTGAAAATAGTCCATCTCTGGCTAGCGTTGACATTGGCTTGAGGGTGAGGGTCATACCGCTATATTTTGGGTCCGAAGAAGGGAATCTAAACAGGGTTATAGGCTGTCTACATGCTATCAAGAAAATGACCCTATCCAGTGCCATGCTAGCGTTTCTGGGCAATAATGACATACCAGACAAACTTCCTACTCTGCTTCGGCATCTGTCAGTTCTCGAACTCAAGGACGTACGGTTGGATTCTTTGATAGAAGTGTTGGTTTGTGTCTGCATTTTTCGAAGTGCTCCAAATTTAGAGGAACTACACCTTTCAGTTGCTGATACAACTGAGTATTCCAGACCTGCTGCTGACTTCCTCATCACTAAGTTGTCAAACCATTACTTCGAAAAACTTAAAGTAGCGAAGATAAGAGCGGTACAAAATGTTCAAAATGAGACGATATTCATCCAGCTCTTACTCGCTCATTCGCCCCTGCTGAAAAGAATGACCATTGTACATTATGGAAGCAGATTTCTTCCAACAGAAGCGCTGGAGCAATCTGTGCCGGCTTCAAAGGATGTTGAAATCTTCAACTTATGCGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box FBD

Protein Analysis

451

Amino Acids

51.18

Weight (kDa)

8.37

Isoelectric Point (pI)

44.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F-box	PF00646	45 - 80	2.8e-06	F-box domain
LRR_At1g61320_AtMIF1	PF23622	117 - 394	1.5e-17	At1g61320/AtMIF1, LRR domain
LRR_At5g56370	PF24758	137 - 358	4.8e-20	FBD-associated F-box protein At5g56370, LRR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016904)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g45851
malus_domestica	MD09G1079300.v1.1 MD17G1069700.v1.1
prunus_persica	Prupe.3G245000_v2.0.a1 Prupe.3G245000_v2.0.a1 Prupe.3G245000_v2.0.a1 Prupe.3G245000_v2.0.a1 Prupe.3G245000_v2.0.a1
pyrus_communis	pycom09g00610 pycom17g06980
rosa_laevigata	RLG00000021436
rosa_rugosa	Rorug02G0506500
rosa_samantha	Rh2DG594900
rosa_wichuraiana	Rw2G047540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	210
Acc36I	ACCTGC	2 cut(s)	210, 1117
AccBSI	CCGCTC	1 cut(s)	1181
AccI	GTMKAC	1 cut(s)	877
AccII	CGCG	1 cut(s)	722
AciI	CCGC	2 cut(s)	827, 1181
AclI	AACGTT	1 cut(s)	484
AclWI	GGATC	2 cut(s)	160, 173
AcoI	YGGCCR	1 cut(s)	392
AcsI	RAATTY	2 cut(s)	324, 1057
AcuI	CTGAAG	1 cut(s)	588
AfaI	GTAC	3 cut(s)	999, 1185, 1263
AfeI	AGCGCT	1 cut(s)	1296
AfiI	CCNNNNNNNGG	1 cut(s)	849
AgeI	ACCGGT	1 cut(s)	353
AluBI	AGCT	6 cut(s)	8, 37, 376, 538, 572, 1219
AluI	AGCT	6 cut(s)	8, 37, 376, 538, 572, 1219
Alw21I	GWGCWC	1 cut(s)	1054
Alw26I	GTCTC	3 cut(s)	220, 449, 1196
AlwI	GGATC	2 cut(s)	160, 173
AlwNI	CAGNNNCTG	1 cut(s)	1085
Aor51HI	AGCGCT	1 cut(s)	1296
AoxI	GGCC	1 cut(s)	392
ApeKI	GCWGC	3 cut(s)	37, 380, 1112
ApoI	RAATTY	2 cut(s)	324, 1057
AsiGI	ACCGGT	1 cut(s)	353
Asp700I	GAANNNNTTC	1 cut(s)	327
AspLEI	GCGC	1 cut(s)	1297
AspS9I	GGNCC	2 cut(s)	356, 840
AsuC2I	CCSGG	1 cut(s)	659
AsuHPI	GGTGA	1 cut(s)	826
AsuII	TTCGAA	2 cut(s)	1045, 1152
AsuNHI	GCTAGC	2 cut(s)	791, 916
AvaII	GGWCC	2 cut(s)	356, 840
BamHI	GGATCC	1 cut(s)	165
BbsI	GAAGAC	1 cut(s)	75
Bbv12I	GWGCWC	1 cut(s)	1054
BbvI	GCAGC	3 cut(s)	24, 367, 1099
BccI	CCATC	5 cut(s)	100, 300, 568, 609, 790
BclI	TGATCA	2 cut(s)	76, 691
BcnI	CCSGG	1 cut(s)	659
BcoDI	GTCTC	3 cut(s)	220, 449, 1196
BfaI	CTAG	5 cut(s)	204, 216, 635, 792, 917
BfmI	CTRYAG	3 cut(s)	16, 360, 461
BfoI	RGCGCY	1 cut(s)	1298
BfuAI	ACCTGC	2 cut(s)	210, 1117
BisI	GCNGC	3 cut(s)	38, 381, 1113
BlsI	GCNGC	3 cut(s)	39, 382, 1114
Bme1390I	CCNGG	1 cut(s)	659
Bme18I	GGWCC	2 cut(s)	356, 840
BmgT120I	GGNCC	2 cut(s)	356, 840
BmiI	GGNNCC	2 cut(s)	167, 841
BmrFI	CCNGG	1 cut(s)	659
BmsI	GCATC	3 cut(s)	216, 355, 979
BmtI	GCTAGC	2 cut(s)	795, 920
BoxI	GACNNNNGTC	1 cut(s)	441
BpiI	GAAGAC	1 cut(s)	75
BpmI	CTGGAG	1 cut(s)	1319
Bpu14I	TTCGAA	2 cut(s)	1045, 1152
BpuEI	CTTGAG	4 cut(s)	431, 713, 829, 974
BpuMI	CCSGG	1 cut(s)	659
Bsa29I	ATCGAT	1 cut(s)	314
BsaBI	GATNNNNATC	2 cut(s)	310, 1211
BsaI	GGTCTC	1 cut(s)	449
BsaJI	CCNNGG	2 cut(s)	146, 657
BsaWI	WCCGGW	1 cut(s)	353
Bsc4I	CCNNNNNNNGG	1 cut(s)	849
Bse118I	RCCGGY	3 cut(s)	353, 390, 1312
Bse1I	ACTGG	4 cut(s)	244, 415, 628, 906
Bse8I	GATNNNNATC	2 cut(s)	310, 1211
BseCI	ATCGAT	1 cut(s)	314
BseDI	CCNNGG	2 cut(s)	146, 657
BseGI	GGATG	4 cut(s)	157, 456, 1212, 1330
BseJI	GATNNNNATC	2 cut(s)	310, 1211
BseLI	CCNNNNNNNGG	1 cut(s)	849
BseMII	CTCAG	3 cut(s)	20, 93, 1086
BseNI	ACTGG	4 cut(s)	244, 415, 628, 906
BseXI	GCAGC	3 cut(s)	24, 367, 1099
Bsh1236I	CGCG	1 cut(s)	722
BshFI	GGCC	1 cut(s)	394
BshTI	ACCGGT	1 cut(s)	353
BshVI	ATCGAT	1 cut(s)	314
BsiHKAI	GWGCWC	1 cut(s)	1054
BsiSI	CCGG	5 cut(s)	283, 354, 391, 659, 1313
BsiWI	CGTACG	1 cut(s)	997
BslI	CCNNNNNNNGG	1 cut(s)	849
BsmAI	GTCTC	3 cut(s)	220, 449, 1196
BsmBI	CGTCTC	1 cut(s)	1196
BsnI	GGCC	1 cut(s)	394
Bso31I	GGTCTC	1 cut(s)	449
Bsp119I	TTCGAA	2 cut(s)	1045, 1152
Bsp1286I	GDGCHC	1 cut(s)	1054
Bsp1407I	TGTACA	1 cut(s)	1261
Bsp143I	GATC	4 cut(s)	76, 165, 315, 691
BspACI	CCGC	2 cut(s)	827, 1181
BspANI	GGCC	1 cut(s)	394
BspCNI	CTCAG	3 cut(s)	21, 94, 1087
BspDI	ATCGAT	1 cut(s)	314
BspFNI	CGCG	1 cut(s)	722
BspLI	GGNNCC	2 cut(s)	167, 841
BspMAI	CTGCAG	2 cut(s)	20, 465
BspMI	ACCTGC	2 cut(s)	210, 1117
BspOI	GCTAGC	2 cut(s)	795, 920
BspPI	GGATC	2 cut(s)	160, 173
BspT104I	TTCGAA	2 cut(s)	1045, 1152
BspTNI	GGTCTC	1 cut(s)	449
BsrBI	CCGCTC	1 cut(s)	1181
BsrFI	RCCGGY	3 cut(s)	353, 390, 1312
BsrGI	TGTACA	1 cut(s)	1261
BsrI	ACTGG	4 cut(s)	244, 415, 628, 906
BssAI	RCCGGY	3 cut(s)	353, 390, 1312
BssECI	CCNNGG	2 cut(s)	146, 657
BssMI	GATC	4 cut(s)	76, 165, 315, 691
BssT1I	CCWWGG	1 cut(s)	146
Bst4CI	ACNGT	3 cut(s)	252, 681, 1002
Bst6I	CTCTTC	1 cut(s)	50
BstAPI	GCANNNNNTGC	1 cut(s)	1309
BstAUI	TGTACA	1 cut(s)	1261
BstBI	TTCGAA	2 cut(s)	1045, 1152
BstC8I	GCNNGC	8 cut(s)	6, 179, 378, 392, 632, 793, 918, 1314
BstDEI	CTNAG	5 cut(s)	29, 102, 707, 1095, 1131
BstF5I	GGATG	4 cut(s)	157, 456, 1212, 1330
BstFNI	CGCG	1 cut(s)	722
BstH2I	RGCGCY	1 cut(s)	1298
BstHHI	GCGC	1 cut(s)	1297
BstKTI	GATC	4 cut(s)	79, 168, 318, 694
BstMAI	GTCTC	3 cut(s)	220, 449, 1196
BstMBI	GATC	4 cut(s)	76, 165, 315, 691
BstMWI	GCNNNNNNNGC	3 cut(s)	279, 917, 1309
BstNSI	RCATGY	2 cut(s)	634, 884
BstPAI	GACNNNNGTC	1 cut(s)	441
BstSCI	CCNGG	1 cut(s)	657
BstSFI	CTRYAG	3 cut(s)	16, 360, 461
BstUI	CGCG	1 cut(s)	722
BstV1I	GCAGC	3 cut(s)	24, 367, 1099
BstV2I	GAAGAC	1 cut(s)	75
BstX2I	RGATCY	1 cut(s)	165
BstYI	RGATCY	1 cut(s)	165
Bsu15I	ATCGAT	1 cut(s)	314
BsuRI	GGCC	1 cut(s)	394
BsuTUI	ATCGAT	1 cut(s)	314
BtsCI	GGATG	4 cut(s)	157, 456, 1212, 1330
BtsIMutI	CAGTG	3 cut(s)	422, 677, 913
BveI	ACCTGC	2 cut(s)	210, 1117
Cac8I	GCNNGC	8 cut(s)	6, 179, 378, 392, 632, 793, 918, 1314
CaiI	CAGNNNCTG	1 cut(s)	1085
CfoI	GCGC	1 cut(s)	1297
Cfr10I	RCCGGY	3 cut(s)	353, 390, 1312
Cfr13I	GGNCC	2 cut(s)	356, 840
ClaI	ATCGAT	1 cut(s)	314
Csp6I	GTAC	3 cut(s)	998, 1184, 1262
CspAI	ACCGGT	1 cut(s)	353
CviAII	CATG	5 cut(s)	319, 513, 631, 881, 913
CviQI	GTAC	3 cut(s)	998, 1184, 1262
DdeI	CTNAG	5 cut(s)	29, 102, 707, 1095, 1131
DpnI	GATC	4 cut(s)	78, 167, 317, 693
DpnII	GATC	4 cut(s)	76, 165, 315, 691
EaeI	YGGCCR	1 cut(s)	392
Eam1104I	CTCTTC	1 cut(s)	50
EarI	CTCTTC	1 cut(s)	50
Eco130I	CCWWGG	1 cut(s)	146
Eco31I	GGTCTC	1 cut(s)	449
Eco32I	GATATC	1 cut(s)	288
Eco47I	GGWCC	2 cut(s)	356, 840
Eco47III	AGCGCT	1 cut(s)	1296
Eco57I	CTGAAG	1 cut(s)	588
EcoRV	GATATC	1 cut(s)	288
EcoT14I	CCWWGG	1 cut(s)	146
EcoT22I	ATGCAT	1 cut(s)	275
ErhI	CCWWGG	1 cut(s)	146
Esp3I	CGTCTC	1 cut(s)	1196
FaeI	CATG	5 cut(s)	322, 516, 634, 884, 916
FatI	CATG	5 cut(s)	318, 512, 630, 880, 912
FbaI	TGATCA	2 cut(s)	76, 691
FblI	GTMKAC	1 cut(s)	877
Fnu4HI	GCNGC	3 cut(s)	38, 381, 1113
FokI	GGATG	4 cut(s)	164, 443, 1199, 1337
Fsp4HI	GCNGC	3 cut(s)	38, 381, 1113
FspBI	CTAG	5 cut(s)	204, 216, 635, 792, 917
GlaI	GCGC	1 cut(s)	1296
GluI	GCNGC	3 cut(s)	38, 381, 1113
GsuI	CTGGAG	1 cut(s)	1319
HaeII	RGCGCY	1 cut(s)	1298
HaeIII	GGCC	1 cut(s)	394
HapII	CCGG	5 cut(s)	283, 354, 391, 659, 1313
HhaI	GCGC	1 cut(s)	1297
Hin1II	CATG	5 cut(s)	322, 516, 634, 884, 916
Hin6I	GCGC	1 cut(s)	1295
HinP1I	GCGC	1 cut(s)	1295
HincII	GTYRAC	1 cut(s)	799
HindII	GTYRAC	1 cut(s)	799
HinfI	GANTC	9 cut(s)	311, 421, 526, 552, 579, 724, 746, 853, 1007
HpaII	CCGG	5 cut(s)	283, 354, 391, 659, 1313
HphI	GGTGA	1 cut(s)	826
Hpy166II	GTNNAC	3 cut(s)	518, 799, 878
Hpy188I	TCNGA	7 cut(s)	30, 292, 406, 568, 578, 738, 844
Hpy188III	TCNNGA	6 cut(s)	583, 665, 770, 889, 983, 1104
Hpy8I	GTNNAC	3 cut(s)	518, 799, 878
HpyAV	CCTTC	1 cut(s)	842
HpyCH4III	ACNGT	3 cut(s)	252, 681, 1002
HpyCH4IV	ACGT	2 cut(s)	484, 996
HpyCH4V	TGCA	5 cut(s)	18, 273, 346, 463, 1038
HpyF10VI	GCNNNNNNNGC	3 cut(s)	279, 917, 1309
HpyF3I	CTNAG	5 cut(s)	29, 102, 707, 1095, 1131
HpySE526I	ACGT	2 cut(s)	484, 996
Hsp92II	CATG	5 cut(s)	322, 516, 634, 884, 916
HspAI	GCGC	1 cut(s)	1295
KroI	GCCGGC	2 cut(s)	390, 1312
KroNI	GCCGGC	2 cut(s)	392, 1314
Ksp22I	TGATCA	2 cut(s)	76, 691
Kzo9I	GATC	4 cut(s)	76, 165, 315, 691
LmnI	GCTCC	3 cut(s)	535, 1057, 1300
Lsp1109I	GCAGC	3 cut(s)	24, 367, 1099
LweI	GCATC	3 cut(s)	216, 355, 979
MaeI	CTAG	5 cut(s)	204, 216, 635, 792, 917
MaeII	ACGT	2 cut(s)	484, 996
MaeIII	GTNAC	2 cut(s)	115, 619
MalI	GATC	4 cut(s)	78, 167, 317, 693
MbiI	CCGCTC	1 cut(s)	1181
MboI	GATC	4 cut(s)	76, 165, 315, 691
MboII	GAAGA	8 cut(s)	67, 75, 320, 555, 857, 1183, 1274, 1327
MfeI	CAATTG	1 cut(s)	341
MflI	RGATCY	1 cut(s)	165
MhlI	GDGCHC	1 cut(s)	1054
MluCI	AATT	7 cut(s)	72, 132, 324, 341, 699, 711, 1057
MmeI	TCCRAC	4 cut(s)	36, 774, 984, 1310
MnlI	CCTC	8 cut(s)	51, 179, 192, 608, 804, 810, 1057, 1133
Mph1103I	ATGCAT	1 cut(s)	275
MroNI	GCCGGC	2 cut(s)	390, 1312
MroXI	GAANNNNTTC	1 cut(s)	327
MseI	TTAA	6 cut(s)	372, 431, 489, 606, 683, 1161
MslI	CAYNNNNRTG	1 cut(s)	606
MspI	CCGG	5 cut(s)	283, 354, 391, 659, 1313
MspR9I	CCNGG	1 cut(s)	659
MunI	CAATTG	1 cut(s)	341
MvnI	CGCG	1 cut(s)	722
MwoI	GCNNNNNNNGC	3 cut(s)	279, 917, 1309
NaeI	GCCGGC	2 cut(s)	392, 1314
NciI	CCSGG	1 cut(s)	659
NdeII	GATC	4 cut(s)	76, 165, 315, 691
NgoMIV	GCCGGC	2 cut(s)	390, 1312
NheI	GCTAGC	2 cut(s)	791, 916
NlaIII	CATG	5 cut(s)	322, 516, 634, 884, 916
NlaIV	GGNNCC	2 cut(s)	167, 841
NsiI	ATGCAT	1 cut(s)	275
NspI	RCATGY	2 cut(s)	634, 884
NspV	TTCGAA	2 cut(s)	1045, 1152
PaeI	GCATGC	1 cut(s)	634
PaqCI	CACCTGC	1 cut(s)	210
PcsI	WCGNNNNNNNCGW	1 cut(s)	168
PdiI	GCCGGC	2 cut(s)	392, 1314
PdmI	GAANNNNTTC	1 cut(s)	327
PfeI	GAWTC	9 cut(s)	311, 421, 526, 552, 579, 724, 746, 853, 1007
Pfl23II	CGTACG	1 cut(s)	997
PflFI	GACNNNGTC	1 cut(s)	296
PinAI	ACCGGT	1 cut(s)	353
PkrI	GCNGC	3 cut(s)	39, 382, 1114
PshAI	GACNNNNGTC	1 cut(s)	441
Psp1406I	AACGTT	1 cut(s)	484
PspLI	CGTACG	1 cut(s)	997
PspN4I	GGNNCC	2 cut(s)	167, 841
PspPI	GGNCC	2 cut(s)	356, 840
PsrI	GAACNNNNNNTAC	2 cut(s)	1176, 1208
PstI	CTGCAG	2 cut(s)	20, 465
PstNI	CAGNNNCTG	1 cut(s)	1085
PsuI	RGATCY	1 cut(s)	165
PsyI	GACNNNGTC	1 cut(s)	296
RsaI	GTAC	3 cut(s)	999, 1185, 1263
RsaNI	GTAC	3 cut(s)	998, 1184, 1262
RseI	CAYNNNNRTG	1 cut(s)	606
SaqAI	TTAA	6 cut(s)	372, 431, 489, 606, 683, 1161
SatI	GCNGC	3 cut(s)	38, 381, 1113
Sau3AI	GATC	4 cut(s)	76, 165, 315, 691
Sau96I	GGNCC	2 cut(s)	356, 840
SbfI	CCTGCAGG	1 cut(s)	465
ScrFI	CCNGG	1 cut(s)	659
SdaI	CCTGCAGG	1 cut(s)	465
SduI	GDGCHC	1 cut(s)	1054
SfaNI	GCATC	3 cut(s)	216, 355, 979
SfcI	CTRYAG	3 cut(s)	16, 360, 461
SfuI	TTCGAA	2 cut(s)	1045, 1152
SinI	GGWCC	2 cut(s)	356, 840
SmiMI	CAYNNNNRTG	1 cut(s)	606
SmlI	CTYRAG	4 cut(s)	446, 728, 808, 989
SmoI	CTYRAG	4 cut(s)	446, 728, 808, 989
SphI	GCATGC	1 cut(s)	634
Sse8387I	CCTGCAGG	1 cut(s)	465
Sse9I	AATT	7 cut(s)	72, 132, 324, 341, 699, 711, 1057
SsiI	CCGC	2 cut(s)	827, 1181
SspMI	CTAG	5 cut(s)	204, 216, 635, 792, 917
StyD4I	CCNGG	1 cut(s)	657
StyI	CCWWGG	1 cut(s)	146
TaaI	ACNGT	3 cut(s)	252, 681, 1002
TaiI	ACGT	2 cut(s)	487, 999
TaqI	TCGA	4 cut(s)	314, 984, 1045, 1152
TasI	AATT	7 cut(s)	72, 132, 324, 341, 699, 711, 1057
TatI	WGTACW	1 cut(s)	1261
TfiI	GAWTC	9 cut(s)	311, 421, 526, 552, 579, 724, 746, 853, 1007
Tru1I	TTAA	6 cut(s)	372, 431, 489, 606, 683, 1161
Tru9I	TTAA	6 cut(s)	372, 431, 489, 606, 683, 1161
TscAI	CASTG	3 cut(s)	422, 684, 913
TseI	GCWGC	3 cut(s)	37, 380, 1112
TspDTI	ATGAA	5 cut(s)	320, 413, 445, 624, 1201
TspRI	CASTG	3 cut(s)	422, 684, 913
Tth111I	GACNNNGTC	1 cut(s)	296
VpaK11BI	GGWCC	2 cut(s)	356, 840
XapI	RAATTY	2 cut(s)	324, 1057
XceI	RCATGY	2 cut(s)	634, 884
XmiI	GTMKAC	1 cut(s)	877
XmnI	GAANNNNTTC	1 cut(s)	327
XspI	CTAG	5 cut(s)	204, 216, 635, 792, 917
Zsp2I	ATGCAT	1 cut(s)	275

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.