MD09G1099100.v1.1

Leucine-rich repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0007873
Physical Location & Seq
Reverse (-)
7230256 .. 7231990
1735 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1099100.v1.1.491

Sequence Viewer

Length: 1299 bp
ATGCAGGTAAAGCAAGAACAGGAAAGAGATGATAATGTAAGTAAAGCTGAGAACGATAATATTGAAAACTCCATCGATGATGGCTATGATTCCTGTAACTACTCTAGTTTTGCAAAGGACTGGATAGTGCCACCTGCAGATGAGGTAAGGGCAGACAAAAACCTCCAAGGTGAATCCTCAGTTCAAAGTTGGGATGAATTGCCGAACAAGGATTTTAAATATAAGCGTATCGAGGAGTGGGTTAATAATCTTCAGCATAGCAGCAGTCCGGTGGAAGAAACGAATGAATTGTCCCAATCTAATGATCAGGTGAAGAGAGAGTCCAATGATTCAAATGGTTTGACTGCTGCTAAGGTGGATGGTAAGGTCACTCCTGGCATGGAAGCTGCTAAGCGATATATATCTTCTCTGAGTGCAGCTGCCACCACAGCTCAGCTTGCAAATCATGGATTGGTAGTGATCCCATTCTTTAGTGCTTTTGTAAATCTCAGGCGTTCCATTTGCAAACTTGCTGCAGTAAGGATAACTGCTGGTGCTCTTCCTCGAGGACTCCATATGTTGAATCTTTCAAAAAACAGTATTTCTACTATCGAAGGTTTGCGTGAACTCACAAGACTTCGTGTACTGGACCTGAGCTATAACAGGATTTTGAGAATTGGACATGGCCTAGCTTCTTGTTCGTCTCTAAAGGAATTGTACTTGGCTGGAAACAAAATCAGTGAGGTGGAGGGTCTTCATCGCCTCCTAAAACTGACTGTTCTGGATCTGCGCTTCAACAAAATCTCAACGGCAAAATGTCTTGGTCAACTGGCGGCTAACTACAATTCCTTACAAGCCATCAGCCTGGAAGGAAACCCAGCTCAGAAGAATGTCGGCGATGACCAATTGAAGAAATACCTGCAAGGACTTCTTCCCCACATGGTTTACTACAACCGGCAGCAAATCAAGTCCAGCGCTCTCAAGGACGCTGCAGATCGGTCAGTACGCTTAGGCATTAGCAACCACCAGTTTGATCGCAGCCTTAGACCCGATCACAAGGCTCCGAGGAAGGCTGGTCACGGTGTATCTTCTAAAAAGCCATCGACTTCATCAACTCACGGTCATGCAAGTCAATCTGTAGCGTCACTGAAAAAGTCTCGGGGCAGGCACAGCCACCTCCCTCCGAGTGGAACAAAAGTAACAGCGCAGAGTCGGCAGCACTACTTTGACTTGGGCAGCAGAATTATGAACCTCAAGCAGGATCTCTCGATGCGCAGGAGTCGGAGTGAGGGAACTATGGCAGCCATCTCAAGTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_6 LRR_8

Protein Analysis

433

Amino Acids

47.73

Weight (kDa)

9.54

Isoelectric Point (pI)

52.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 182 - 216 4.9e-06 Leucine rich repeat
LRR_4 PF12799 184 - 217 7.6e-07 Leucine Rich repeats (2 copies)
LRR_9 PF14580 186 - 263 6.4e-08 Leucine-rich repeat
LRR_8 PF13855 205 - 261 2.2e-09 Leucine rich repeat
LRR_4 PF12799 228 - 263 7.3e-07 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 142
AbsI CCTCGAGG 1 cut(s) 543
Acc16I TGCGCA 1 cut(s) 1253
Acc36I ACCTGC 2 cut(s) 142, 906
AciI CCGC 1 cut(s) 812
AclWI GGATC 3 cut(s) 454, 771, 1248
AcuI CTGAAG 1 cut(s) 236
AfaI GTAC 3 cut(s) 624, 698, 984
AfeI AGCGCT 1 cut(s) 955
AfiI CCNNNNNNNGG 2 cut(s) 1166, 1237
AgsI TTSAA 7 cut(s) 65, 185, 333, 562, 570, 775, 889
AjnI CCWGG 2 cut(s) 373, 843
AluBI AGCT 8 cut(s) 47, 386, 419, 431, 436, 636, 671, 860
AluI AGCT 8 cut(s) 47, 386, 419, 431, 436, 636, 671, 860
Alw21I GWGCWC 1 cut(s) 538
Alw26I GTCTC 2 cut(s) 687, 1140
AlwI GGATC 3 cut(s) 454, 771, 1248
Ama87I CYCGRG 2 cut(s) 543, 1137
Aor51HI AGCGCT 1 cut(s) 955
AoxI GGCC 1 cut(s) 664
ArsI GACNNNNNNTTYG 2 cut(s) 275, 307
AspLEI GCGC 4 cut(s) 771, 956, 1186, 1254
AspS9I GGNCC 1 cut(s) 628
AsuHPI GGTGA 2 cut(s) 182, 322
AvaI CYCGRG 2 cut(s) 543, 1137
AvaII GGWCC 1 cut(s) 628
BbsI GAAGAC 1 cut(s) 725
Bbv12I GWGCWC 1 cut(s) 538
BccI CCATC 6 cut(s) 74, 80, 353, 845, 1087, 1292
BceAI ACGGC 1 cut(s) 804
BciT130I CCWGG 2 cut(s) 375, 845
BclI TGATCA 1 cut(s) 304
BcoDI GTCTC 2 cut(s) 687, 1140
BfaI CTAG 2 cut(s) 105, 668
BfmI CTRYAG 4 cut(s) 135, 513, 969, 1116
BfoI RGCGCY 1 cut(s) 957
BfuAI ACCTGC 2 cut(s) 142, 906
BlpI GCTNAGC 2 cut(s) 390, 432
Bme1390I CCNGG 2 cut(s) 375, 845
Bme18I GGWCC 1 cut(s) 628
BmeT110I CYCGRG 2 cut(s) 543, 1137
BmgT120I GGNCC 1 cut(s) 628
BmiI GGNNCC 1 cut(s) 1041
BmrFI CCNGG 2 cut(s) 375, 845
BmsI GCATC 1 cut(s) 1239
BpiI GAAGAC 1 cut(s) 725
Bpu10I CCTNAGC 3 cut(s) 351, 632, 988
Bpu1102I GCTNAGC 2 cut(s) 390, 432
BpuEI CTTGAG 3 cut(s) 944, 1217, 1273
Bsa29I ATCGAT 1 cut(s) 75
BsaBI GATNNNNATC 1 cut(s) 400
BsaJI CCNNGG 2 cut(s) 166, 1043
BsaWI WCCGGW 1 cut(s) 268
BsaXI ACNNNNNCTCC 2 cut(s) 1249, 1279
Bsc4I CCNNNNNNNGG 2 cut(s) 1166, 1237
Bse118I RCCGGY 1 cut(s) 933
Bse1I ACTGG 4 cut(s) 125, 630, 813, 1006
Bse8I GATNNNNATC 1 cut(s) 400
BseBI CCWGG 2 cut(s) 375, 845
BseCI ATCGAT 1 cut(s) 75
BseDI CCNNGG 2 cut(s) 166, 1043
BseGI GGATG 2 cut(s) 199, 364
BseJI GATNNNNATC 1 cut(s) 400
BseLI CCNNNNNNNGG 2 cut(s) 1166, 1237
BseMII CTCAG 7 cut(s) 39, 192, 401, 446, 502, 623, 875
BseNI ACTGG 4 cut(s) 125, 630, 813, 1006
BseRI GAGGAG 1 cut(s) 248
BseYI CCCAGC 1 cut(s) 856
BsgI GTGCAG 1 cut(s) 435
BshFI GGCC 1 cut(s) 666
BshVI ATCGAT 1 cut(s) 75
BsiHKAI GWGCWC 1 cut(s) 538
BsiHKCI CYCGRG 2 cut(s) 543, 1137
BsiSI CCGG 2 cut(s) 269, 934
BslFI GGGAC 1 cut(s) 277
BslI CCNNNNNNNGG 2 cut(s) 1166, 1237
BsmAI GTCTC 2 cut(s) 687, 1140
BsmBI CGTCTC 1 cut(s) 687
BsmFI GGGAC 1 cut(s) 277
BsnI GGCC 1 cut(s) 666
BsoBI CYCGRG 2 cut(s) 543, 1137
Bsp1286I GDGCHC 1 cut(s) 538
Bsp143I GATC 7 cut(s) 304, 459, 763, 973, 1012, 1030, 1240
Bsp1720I GCTNAGC 2 cut(s) 390, 432
BspACI CCGC 1 cut(s) 812
BspANI GGCC 1 cut(s) 666
BspCNI CTCAG 7 cut(s) 40, 191, 402, 445, 501, 624, 874
BspDI ATCGAT 1 cut(s) 75
BspLI GGNNCC 1 cut(s) 1041
BspMAI CTGCAG 3 cut(s) 139, 517, 973
BspMI ACCTGC 2 cut(s) 142, 906
BspPI GGATC 3 cut(s) 454, 771, 1248
BspQI GCTCTTC 1 cut(s) 543
BsrFI RCCGGY 1 cut(s) 933
BsrI ACTGG 4 cut(s) 125, 630, 813, 1006
BssAI RCCGGY 1 cut(s) 933
BssECI CCNNGG 2 cut(s) 166, 1043
BssMI GATC 7 cut(s) 304, 459, 763, 973, 1012, 1030, 1240
BssT1I CCWWGG 1 cut(s) 166
Bst2UI CCWGG 2 cut(s) 375, 845
Bst4CI ACNGT 4 cut(s) 578, 757, 1061, 1100
Bst6I CTCTTC 2 cut(s) 308, 543
BstC8I GCNNGC 2 cut(s) 438, 1145
BstENI CCTNNNNNAGG 1 cut(s) 1235
BstF5I GGATG 2 cut(s) 199, 364
BstH2I RGCGCY 1 cut(s) 957
BstHHI GCGC 4 cut(s) 771, 956, 1186, 1254
BstKTI GATC 7 cut(s) 307, 462, 766, 976, 1015, 1033, 1243
BstMAI GTCTC 2 cut(s) 687, 1140
BstMBI GATC 7 cut(s) 304, 459, 763, 973, 1012, 1030, 1240
BstMWI GCNNNNNNNGC 5 cut(s) 10, 428, 437, 1149, 1192
BstNI CCWGG 2 cut(s) 375, 845
BstSCI CCNGG 2 cut(s) 373, 843
BstSFI CTRYAG 4 cut(s) 135, 513, 969, 1116
BstV2I GAAGAC 1 cut(s) 725
BstX2I RGATCY 2 cut(s) 763, 1240
BstXI CCANNNNNNTGG 1 cut(s) 844
BstYI RGATCY 2 cut(s) 763, 1240
Bsu15I ATCGAT 1 cut(s) 75
BsuRI GGCC 1 cut(s) 666
BsuTUI ATCGAT 1 cut(s) 75
BtgZI GCGATG 2 cut(s) 722, 891
BtsCI GGATG 2 cut(s) 199, 364
BtsIMutI CAGTG 2 cut(s) 724, 1124
BveI ACCTGC 2 cut(s) 142, 906
Cac8I GCNNGC 2 cut(s) 438, 1145
CfoI GCGC 4 cut(s) 771, 956, 1186, 1254
Cfr10I RCCGGY 1 cut(s) 933
Cfr13I GGNCC 1 cut(s) 628
ClaI ATCGAT 1 cut(s) 75
CseI GACGC 2 cut(s) 974, 1110
Csp6I GTAC 3 cut(s) 623, 697, 983
CviAII CATG 5 cut(s) 379, 446, 662, 919, 1103
CviQI GTAC 3 cut(s) 623, 697, 983
DpnI GATC 7 cut(s) 306, 461, 765, 975, 1014, 1032, 1242
DpnII GATC 7 cut(s) 304, 459, 763, 973, 1012, 1030, 1240
DraI TTTAAA 1 cut(s) 217
Eam1104I CTCTTC 2 cut(s) 308, 543
EarI CTCTTC 2 cut(s) 308, 543
Eco130I CCWWGG 1 cut(s) 166
Eco47I GGWCC 1 cut(s) 628
Eco47III AGCGCT 1 cut(s) 955
Eco57I CTGAAG 1 cut(s) 236
Eco88I CYCGRG 2 cut(s) 543, 1137
EcoNI CCTNNNNNAGG 1 cut(s) 1235
EcoRII CCWGG 2 cut(s) 373, 843
EcoT14I CCWWGG 1 cut(s) 166
ErhI CCWWGG 1 cut(s) 166
Esp3I CGTCTC 1 cut(s) 687
FaeI CATG 5 cut(s) 382, 449, 665, 922, 1106
FalI AAGNNNNNCTT 2 cut(s) 894, 926
FaqI GGGAC 1 cut(s) 277
FatI CATG 5 cut(s) 378, 445, 661, 918, 1102
FauNDI CATATG 1 cut(s) 555
FbaI TGATCA 1 cut(s) 304
FokI GGATG 2 cut(s) 206, 371
FspBI CTAG 2 cut(s) 105, 668
FspI TGCGCA 1 cut(s) 1253
GlaI GCGC 4 cut(s) 770, 955, 1185, 1253
GsaI CCCAGC 1 cut(s) 860
HaeII RGCGCY 1 cut(s) 957
HaeIII GGCC 1 cut(s) 666
HapII CCGG 2 cut(s) 269, 934
HgaI GACGC 2 cut(s) 974, 1110
HhaI GCGC 4 cut(s) 771, 956, 1186, 1254
Hin1II CATG 5 cut(s) 382, 449, 665, 922, 1106
Hin6I GCGC 4 cut(s) 769, 954, 1184, 1252
HinP1I GCGC 4 cut(s) 769, 954, 1184, 1252
HincII GTYRAC 1 cut(s) 806
HindII GTYRAC 1 cut(s) 806
HinfI GANTC 8 cut(s) 89, 173, 320, 329, 549, 562, 1189, 1258
HpaII CCGG 2 cut(s) 269, 934
HphI GGTGA 2 cut(s) 182, 322
Hpy166II GTNNAC 4 cut(s) 605, 623, 806, 925
Hpy188I TCNGA 5 cut(s) 411, 864, 1044, 1164, 1263
Hpy188III TCNNGA 2 cut(s) 761, 1246
Hpy8I GTNNAC 4 cut(s) 605, 623, 806, 925
HpyAV CCTTC 3 cut(s) 587, 842, 1042
HpyCH4III ACNGT 4 cut(s) 578, 757, 1061, 1100
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 428, 437, 1149, 1192
Hsp92II CATG 5 cut(s) 382, 449, 665, 922, 1106
HspAI GCGC 4 cut(s) 769, 954, 1184, 1252
Ksp22I TGATCA 1 cut(s) 304
Kzo9I GATC 7 cut(s) 304, 459, 763, 973, 1012, 1030, 1240
LguI GCTCTTC 1 cut(s) 543
LmnI GCTCC 1 cut(s) 1045
LweI GCATC 1 cut(s) 1239
MaeI CTAG 2 cut(s) 105, 668
MaeIII GTNAC 5 cut(s) 95, 367, 1055, 1122, 1177
MalI GATC 7 cut(s) 306, 461, 765, 975, 1014, 1032, 1242
MboI GATC 7 cut(s) 304, 459, 763, 973, 1012, 1030, 1240
MfeI CAATTG 1 cut(s) 884
MflI RGATCY 2 cut(s) 763, 1240
MhlI GDGCHC 1 cut(s) 538
MluCI AATT 7 cut(s) 197, 287, 654, 692, 823, 884, 1221
MlyI GAGTC 4 cut(s) 329, 543, 1198, 1267
MmeI TCCRAC 1 cut(s) 1241
MseI TTAA 3 cut(s) 216, 243, 1297
MslI CAYNNNNRTG 1 cut(s) 1101
MspA1I CMGCKG 1 cut(s) 419
MspI CCGG 2 cut(s) 269, 934
MspR9I CCNGG 2 cut(s) 375, 845
MunI CAATTG 1 cut(s) 884
MvaI CCWGG 2 cut(s) 375, 845
MwoI GCNNNNNNNGC 5 cut(s) 10, 428, 437, 1149, 1192
NdeI CATATG 1 cut(s) 555
NdeII GATC 7 cut(s) 304, 459, 763, 973, 1012, 1030, 1240
NlaIII CATG 5 cut(s) 382, 449, 665, 922, 1106
NlaIV GGNNCC 1 cut(s) 1041
NmuCI GTSAC 3 cut(s) 367, 1055, 1122
NsbI TGCGCA 1 cut(s) 1253
PaeR7I CTCGAG 1 cut(s) 543
PaqCI CACCTGC 1 cut(s) 142
PciSI GCTCTTC 1 cut(s) 543
PfeI GAWTC 4 cut(s) 89, 173, 329, 562
PleI GAGTC 4 cut(s) 328, 543, 1197, 1266
PpsI GAGTC 4 cut(s) 328, 543, 1197, 1266
Psp6I CCWGG 2 cut(s) 373, 843
PspFI CCCAGC 1 cut(s) 856
PspGI CCWGG 2 cut(s) 373, 843
PspN4I GGNNCC 1 cut(s) 1041
PspPI GGNCC 1 cut(s) 628
PspXI VCTCGAGB 1 cut(s) 543
PstI CTGCAG 3 cut(s) 139, 517, 973
PsuI RGATCY 2 cut(s) 763, 1240
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 3 cut(s) 624, 698, 984
RsaNI GTAC 3 cut(s) 623, 697, 983
RseI CAYNNNNRTG 1 cut(s) 1101
SapI GCTCTTC 1 cut(s) 543
SaqAI TTAA 3 cut(s) 216, 243, 1297
Sau3AI GATC 7 cut(s) 304, 459, 763, 973, 1012, 1030, 1240
Sau96I GGNCC 1 cut(s) 628
SchI GAGTC 4 cut(s) 329, 543, 1198, 1267
ScrFI CCNGG 2 cut(s) 375, 845
SduI GDGCHC 1 cut(s) 538
SfaNI GCATC 1 cut(s) 1239
SfcI CTRYAG 4 cut(s) 135, 513, 969, 1116
Sfr274I CTCGAG 1 cut(s) 543
SinI GGWCC 1 cut(s) 628
SlaI CTCGAG 1 cut(s) 543
SmiMI CAYNNNNRTG 1 cut(s) 1101
SmlI CTYRAG 4 cut(s) 543, 959, 1232, 1288
SmoI CTYRAG 4 cut(s) 543, 959, 1232, 1288
Sse9I AATT 7 cut(s) 197, 287, 654, 692, 823, 884, 1221
SsiI CCGC 1 cut(s) 812
SspI AATATT 1 cut(s) 61
SspMI CTAG 2 cut(s) 105, 668
StyD4I CCNGG 2 cut(s) 373, 843
StyI CCWWGG 1 cut(s) 166
TaaI ACNGT 4 cut(s) 578, 757, 1061, 1100
TaqI TCGA 6 cut(s) 75, 231, 544, 591, 1082, 1247
TaqII GACCGA 1 cut(s) 966
TasI AATT 7 cut(s) 197, 287, 654, 692, 823, 884, 1221
TatI WGTACW 2 cut(s) 622, 696
TauI GCSGC 1 cut(s) 815
TfiI GAWTC 4 cut(s) 89, 173, 329, 562
Tru1I TTAA 3 cut(s) 216, 243, 1297
Tru9I TTAA 3 cut(s) 216, 243, 1297
TscAI CASTG 2 cut(s) 724, 1131
TseFI GTSAC 3 cut(s) 367, 1055, 1122
Tsp45I GTSAC 3 cut(s) 367, 1055, 1122
TspDTI ATGAA 5 cut(s) 210, 300, 725, 1077, 1241
TspRI CASTG 2 cut(s) 724, 1131
VpaK11BI GGWCC 1 cut(s) 628
XagI CCTNNNNNAGG 1 cut(s) 1235
XhoI CTCGAG 1 cut(s) 543
XspI CTAG 2 cut(s) 105, 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.