pycom09g02280

Leucine-rich repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0007873
Physical Location & Seq
Reverse (-)
1738097 .. 1739690
1594 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g02280.1

Sequence Viewer

Length: 1329 bp
ATGAGGGGGAAAGGGAATTCAGTACATGAGACTGAAATGCAGGTAAAGCAAGAACAAGAAAGAGATGATATTGTAAGTAAAGCTGAGAACGATAATATTGAAAACTCCATCGATGATGGCTATGATTCCTGTAACTACTCTAGTTTTGCAAAGGACTGGATAATGCCACCTGCAGATGAGATAAGGGCAGAGAAAAACCTCCAAGGAGAAACCTCAGTTCAAAGTTGGGATGAATTGCCAAGCAAGGATTTTAAATATAAGCGTATCGAGGAGTGGGTTAATAATCTTCAGCATAGCAGCATTCTGGTGGAAGAAACGAATGAATTGTCCCAATCTAATGATCAGGTGAAGAGAGAGTCCAATGATTCAAACAGTTTGACTGCTGCTAAGGTGGATGGTAAGGTCACTCCTGGCATGGAAGCTGCTAAGCGATATATATCTTCTCTGAGTGCAGCTGCCACCACAGCTCAGCTTGCAAATCATGGATTGGTATCGATCCCATTCCTTAGTGCTTTTGTAAATCTCAGGGTGCTCAATTTATCAGGAAATTCCATAGTAAGGATAACTGCTGGAGCTCTTCCTCGAGGACTCCATATGTTGAATCTTTCAAAAAACGGTATTTCTACTATCGAAGGTTTGCGTGAACTCACAAGACTTCGTGTACTGGACCTGAGCTATAACAGGATTTTGAGAATCGGACATGGCCTAGCTTCTTGTTCGTCTCTAAAGGAATTGTACTTGGCTGGAAACAAAATCAGTGAGGTGGAGGGTCTTCATCGCCTCCTAAAACTGACTGTTCTGGATCTGCGCTTCAACAAAATCTCAACGGCAAAATGTCTTGGTCAACTGGCGGCTAACTACAATTCCTTACAAGCCATCAGCCTGGAAGGAAACCCAGCTCAGAAGAATGTCGGCGATGACCAATTGAAGAAATACCTGCAAGGACTTCTTCCCCACATGGTTTACTACAACCGGCAGCAAATCAAGTCCAGCACTCTCAAGGACGCTGCAGATCGGTCAGTACGCTTAGGCATTAGCAACCACCAGTTTGATCGCAGCCTTAGACTCGATCACAAGGCTCCGAGGAAGGCTGGTCACGGTGTATCTTCTAAAAAGCCATCGACTTCATCAACTCACGGTCATGCAAGTCAATCTGTAGCGTCACTGAAAAAGTCTCGGGGCAGGCACAGCCACCTCCCTCCGAGTGGAACAAAAGTAACATCGCAGAGTCGGCAGCACTACTTCGACTTGGGCAGCAGAATTATGAACCTCAAGCAGGATCTCTCGATGCGCAGGAGTCGGAGTGAGGGAACTATGGCAGCCGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_6 LRR_8

Protein Analysis

443

Amino Acids

48.97

Weight (kDa)

9.48

Isoelectric Point (pI)

47.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 174 - 229 1.1e-09 Leucine rich repeat
LRR_9 PF14580 174 - 276 4.3e-09 Leucine-rich repeat
LRR_4 PF12799 197 - 230 1.3e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 218 - 274 2.5e-09 Leucine rich repeat
LRR_4 PF12799 241 - 276 7.6e-07 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 178
AbsI CCTCGAGG 1 cut(s) 582
Acc16I TGCGCA 1 cut(s) 1292
Acc36I ACCTGC 3 cut(s) 31, 178, 945
AciI CCGC 1 cut(s) 851
AclWI GGATC 3 cut(s) 490, 810, 1287
AcsI RAATTY 2 cut(s) 16, 547
AcuI CTGAAG 1 cut(s) 272
AfaI GTAC 4 cut(s) 24, 663, 737, 1023
AfiI CCNNNNNNNGG 3 cut(s) 558, 1205, 1276
AgsI TTSAA 7 cut(s) 101, 221, 369, 601, 609, 814, 928
AjnI CCWGG 2 cut(s) 409, 882
AluBI AGCT 9 cut(s) 83, 422, 455, 467, 472, 575, 675, 710, 899
AluI AGCT 9 cut(s) 83, 422, 455, 467, 472, 575, 675, 710, 899
Alw21I GWGCWC 2 cut(s) 534, 577
Alw26I GTCTC 3 cut(s) 23, 726, 1179
AlwI GGATC 3 cut(s) 490, 810, 1287
Ama87I CYCGRG 2 cut(s) 582, 1176
AoxI GGCC 1 cut(s) 703
ApoI RAATTY 2 cut(s) 16, 547
ArsI GACNNNNNNTTYG 2 cut(s) 311, 343
AspLEI GCGC 2 cut(s) 810, 1293
AspS9I GGNCC 1 cut(s) 667
AsuHPI GGTGA 1 cut(s) 358
AvaI CYCGRG 2 cut(s) 582, 1176
AvaII GGWCC 1 cut(s) 667
BanII GRGCYC 1 cut(s) 577
BbsI GAAGAC 1 cut(s) 764
Bbv12I GWGCWC 2 cut(s) 534, 577
BccI CCATC 5 cut(s) 110, 116, 389, 884, 1126
BceAI ACGGC 2 cut(s) 843, 1307
BciT130I CCWGG 2 cut(s) 411, 884
BclI TGATCA 1 cut(s) 340
BcoDI GTCTC 3 cut(s) 23, 726, 1179
BfaI CTAG 2 cut(s) 141, 707
BfmI CTRYAG 3 cut(s) 171, 1008, 1155
BfuAI ACCTGC 3 cut(s) 31, 178, 945
BlpI GCTNAGC 2 cut(s) 426, 468
Bme1390I CCNGG 2 cut(s) 411, 884
Bme18I GGWCC 1 cut(s) 667
BmeT110I CYCGRG 2 cut(s) 582, 1176
BmgT120I GGNCC 1 cut(s) 667
BmiI GGNNCC 1 cut(s) 1080
BmrFI CCNGG 2 cut(s) 411, 884
BmsI GCATC 1 cut(s) 1278
BpiI GAAGAC 1 cut(s) 764
BpmI CTGGAG 1 cut(s) 591
Bpu10I CCTNAGC 3 cut(s) 387, 671, 1027
Bpu1102I GCTNAGC 2 cut(s) 426, 468
BpuEI CTTGAG 2 cut(s) 983, 1256
Bsa29I ATCGAT 2 cut(s) 111, 494
BsaBI GATNNNNATC 2 cut(s) 436, 490
BsaJI CCNNGG 2 cut(s) 202, 1082
BsaXI ACNNNNNCTCC 2 cut(s) 1288, 1318
Bsc4I CCNNNNNNNGG 3 cut(s) 558, 1205, 1276
Bse118I RCCGGY 1 cut(s) 972
Bse1I ACTGG 4 cut(s) 161, 669, 852, 1045
Bse8I GATNNNNATC 2 cut(s) 436, 490
BseBI CCWGG 2 cut(s) 411, 884
BseCI ATCGAT 2 cut(s) 111, 494
BseDI CCNNGG 2 cut(s) 202, 1082
BseGI GGATG 2 cut(s) 235, 400
BseJI GATNNNNATC 2 cut(s) 436, 490
BseLI CCNNNNNNNGG 3 cut(s) 558, 1205, 1276
BseMII CTCAG 7 cut(s) 75, 228, 437, 482, 538, 662, 914
BseNI ACTGG 4 cut(s) 161, 669, 852, 1045
BseRI GAGGAG 1 cut(s) 284
BseYI CCCAGC 1 cut(s) 895
BsgI GTGCAG 1 cut(s) 471
BshFI GGCC 1 cut(s) 705
BshVI ATCGAT 2 cut(s) 111, 494
BsiHKAI GWGCWC 2 cut(s) 534, 577
BsiHKCI CYCGRG 2 cut(s) 582, 1176
BsiSI CCGG 1 cut(s) 973
BslFI GGGAC 1 cut(s) 313
BslI CCNNNNNNNGG 3 cut(s) 558, 1205, 1276
BsmAI GTCTC 3 cut(s) 23, 726, 1179
BsmBI CGTCTC 1 cut(s) 726
BsmFI GGGAC 1 cut(s) 313
BsmI GAATGC 1 cut(s) 300
BsnI GGCC 1 cut(s) 705
BsoBI CYCGRG 2 cut(s) 582, 1176
Bsp1286I GDGCHC 2 cut(s) 534, 577
Bsp143I GATC 7 cut(s) 340, 495, 802, 1012, 1051, 1069, 1279
Bsp1720I GCTNAGC 2 cut(s) 426, 468
BspACI CCGC 1 cut(s) 851
BspANI GGCC 1 cut(s) 705
BspCNI CTCAG 7 cut(s) 76, 227, 438, 481, 537, 663, 913
BspDI ATCGAT 2 cut(s) 111, 494
BspLI GGNNCC 1 cut(s) 1080
BspMAI CTGCAG 2 cut(s) 175, 1012
BspMI ACCTGC 3 cut(s) 31, 178, 945
BspPI GGATC 3 cut(s) 490, 810, 1287
BspQI GCTCTTC 1 cut(s) 582
BsrFI RCCGGY 1 cut(s) 972
BsrI ACTGG 4 cut(s) 161, 669, 852, 1045
BssAI RCCGGY 1 cut(s) 972
BssECI CCNNGG 2 cut(s) 202, 1082
BssMI GATC 7 cut(s) 340, 495, 802, 1012, 1051, 1069, 1279
BssT1I CCWWGG 1 cut(s) 202
Bst2UI CCWGG 2 cut(s) 411, 884
Bst4CI ACNGT 5 cut(s) 374, 617, 796, 1100, 1139
Bst6I CTCTTC 2 cut(s) 344, 582
BstC8I GCNNGC 2 cut(s) 474, 1184
BstENI CCTNNNNNAGG 1 cut(s) 1274
BstF5I GGATG 2 cut(s) 235, 400
BstHHI GCGC 2 cut(s) 810, 1293
BstKTI GATC 7 cut(s) 343, 498, 805, 1015, 1054, 1072, 1282
BstMAI GTCTC 3 cut(s) 23, 726, 1179
BstMBI GATC 7 cut(s) 340, 495, 802, 1012, 1051, 1069, 1279
BstMWI GCNNNNNNNGC 5 cut(s) 46, 464, 473, 1188, 1231
BstNI CCWGG 2 cut(s) 411, 884
BstSCI CCNGG 2 cut(s) 409, 882
BstSFI CTRYAG 3 cut(s) 171, 1008, 1155
BstV2I GAAGAC 1 cut(s) 764
BstX2I RGATCY 2 cut(s) 802, 1279
BstXI CCANNNNNNTGG 1 cut(s) 883
BstYI RGATCY 2 cut(s) 802, 1279
Bsu15I ATCGAT 2 cut(s) 111, 494
BsuRI GGCC 1 cut(s) 705
BsuTUI ATCGAT 2 cut(s) 111, 494
BtgZI GCGATG 3 cut(s) 761, 930, 1206
BtsCI GGATG 2 cut(s) 235, 400
BtsIMutI CAGTG 2 cut(s) 763, 1163
BveI ACCTGC 3 cut(s) 31, 178, 945
Cac8I GCNNGC 2 cut(s) 474, 1184
CfoI GCGC 2 cut(s) 810, 1293
Cfr10I RCCGGY 1 cut(s) 972
Cfr13I GGNCC 1 cut(s) 667
ClaI ATCGAT 2 cut(s) 111, 494
CseI GACGC 2 cut(s) 1013, 1149
Csp6I GTAC 4 cut(s) 23, 662, 736, 1022
CviAII CATG 6 cut(s) 26, 415, 482, 701, 958, 1142
CviQI GTAC 4 cut(s) 23, 662, 736, 1022
DpnI GATC 7 cut(s) 342, 497, 804, 1014, 1053, 1071, 1281
DpnII GATC 7 cut(s) 340, 495, 802, 1012, 1051, 1069, 1279
DraI TTTAAA 1 cut(s) 253
Eam1104I CTCTTC 2 cut(s) 344, 582
EarI CTCTTC 2 cut(s) 344, 582
Ecl136II GAGCTC 1 cut(s) 575
Eco130I CCWWGG 1 cut(s) 202
Eco24I GRGCYC 1 cut(s) 577
Eco47I GGWCC 1 cut(s) 667
Eco53kI GAGCTC 1 cut(s) 575
Eco57I CTGAAG 1 cut(s) 272
Eco88I CYCGRG 2 cut(s) 582, 1176
EcoICRI GAGCTC 1 cut(s) 575
EcoNI CCTNNNNNAGG 1 cut(s) 1274
EcoRI GAATTC 1 cut(s) 16
EcoRII CCWGG 2 cut(s) 409, 882
EcoT14I CCWWGG 1 cut(s) 202
EcoT38I GRGCYC 1 cut(s) 577
ErhI CCWWGG 1 cut(s) 202
Esp3I CGTCTC 1 cut(s) 726
FaeI CATG 6 cut(s) 29, 418, 485, 704, 961, 1145
FalI AAGNNNNNCTT 2 cut(s) 933, 965
FaqI GGGAC 1 cut(s) 313
FatI CATG 6 cut(s) 25, 414, 481, 700, 957, 1141
FauNDI CATATG 1 cut(s) 594
FbaI TGATCA 1 cut(s) 340
FokI GGATG 2 cut(s) 242, 407
FriOI GRGCYC 1 cut(s) 577
FspBI CTAG 2 cut(s) 141, 707
FspI TGCGCA 1 cut(s) 1292
GlaI GCGC 2 cut(s) 809, 1292
GsaI CCCAGC 1 cut(s) 899
GsuI CTGGAG 1 cut(s) 591
HaeIII GGCC 1 cut(s) 705
HapII CCGG 1 cut(s) 973
HgaI GACGC 2 cut(s) 1013, 1149
HhaI GCGC 2 cut(s) 810, 1293
Hin1II CATG 6 cut(s) 29, 418, 485, 704, 961, 1145
Hin6I GCGC 2 cut(s) 808, 1291
HinP1I GCGC 2 cut(s) 808, 1291
HincII GTYRAC 1 cut(s) 845
HindII GTYRAC 1 cut(s) 845
HinfI GANTC 9 cut(s) 125, 356, 365, 588, 601, 693, 1065, 1228, 1297
HpaII CCGG 1 cut(s) 973
HphI GGTGA 1 cut(s) 358
Hpy166II GTNNAC 4 cut(s) 644, 662, 845, 964
Hpy188I TCNGA 6 cut(s) 447, 698, 903, 1083, 1203, 1302
Hpy188III TCNNGA 3 cut(s) 543, 800, 1285
Hpy8I GTNNAC 4 cut(s) 644, 662, 845, 964
HpyAV CCTTC 3 cut(s) 626, 881, 1081
HpyCH4III ACNGT 5 cut(s) 374, 617, 796, 1100, 1139
HpyCH4V TGCA 8 cut(s) 40, 149, 173, 452, 476, 940, 1010, 1145
HpyF10VI GCNNNNNNNGC 5 cut(s) 46, 464, 473, 1188, 1231
Hsp92II CATG 6 cut(s) 29, 418, 485, 704, 961, 1145
HspAI GCGC 2 cut(s) 808, 1291
Ksp22I TGATCA 1 cut(s) 340
Kzo9I GATC 7 cut(s) 340, 495, 802, 1012, 1051, 1069, 1279
LguI GCTCTTC 1 cut(s) 582
LmnI GCTCC 2 cut(s) 572, 1084
LweI GCATC 1 cut(s) 1278
MaeI CTAG 2 cut(s) 141, 707
MaeIII GTNAC 5 cut(s) 131, 403, 1094, 1161, 1216
MalI GATC 7 cut(s) 342, 497, 804, 1014, 1053, 1071, 1281
MboI GATC 7 cut(s) 340, 495, 802, 1012, 1051, 1069, 1279
MfeI CAATTG 1 cut(s) 923
MflI RGATCY 2 cut(s) 802, 1279
MhlI GDGCHC 2 cut(s) 534, 577
MluCI AATT 9 cut(s) 16, 233, 323, 535, 547, 731, 862, 923, 1260
MlyI GAGTC 5 cut(s) 365, 582, 1059, 1237, 1306
MmeI TCCRAC 1 cut(s) 1280
MseI TTAA 2 cut(s) 252, 279
MslI CAYNNNNRTG 2 cut(s) 305, 1140
MspA1I CMGCKG 1 cut(s) 455
MspI CCGG 1 cut(s) 973
MspR9I CCNGG 2 cut(s) 411, 884
MunI CAATTG 1 cut(s) 923
Mva1269I GAATGC 1 cut(s) 300
MvaI CCWGG 2 cut(s) 411, 884
MwoI GCNNNNNNNGC 5 cut(s) 46, 464, 473, 1188, 1231
NdeI CATATG 1 cut(s) 594
NdeII GATC 7 cut(s) 340, 495, 802, 1012, 1051, 1069, 1279
NlaIII CATG 6 cut(s) 29, 418, 485, 704, 961, 1145
NlaIV GGNNCC 1 cut(s) 1080
NmuCI GTSAC 3 cut(s) 403, 1094, 1161
NsbI TGCGCA 1 cut(s) 1292
PaeR7I CTCGAG 1 cut(s) 582
PaqCI CACCTGC 1 cut(s) 178
PciSI GCTCTTC 1 cut(s) 582
PctI GAATGC 1 cut(s) 300
PfeI GAWTC 4 cut(s) 125, 365, 601, 693
PleI GAGTC 5 cut(s) 364, 582, 1059, 1236, 1305
PpsI GAGTC 5 cut(s) 364, 582, 1059, 1236, 1305
Psp124BI GAGCTC 1 cut(s) 577
Psp6I CCWGG 2 cut(s) 409, 882
PspFI CCCAGC 1 cut(s) 895
PspGI CCWGG 2 cut(s) 409, 882
PspN4I GGNNCC 1 cut(s) 1080
PspPI GGNCC 1 cut(s) 667
PspXI VCTCGAGB 1 cut(s) 582
PstI CTGCAG 2 cut(s) 175, 1012
PsuI RGATCY 2 cut(s) 802, 1279
PvuII CAGCTG 1 cut(s) 455
RsaI GTAC 4 cut(s) 24, 663, 737, 1023
RsaNI GTAC 4 cut(s) 23, 662, 736, 1022
RseI CAYNNNNRTG 2 cut(s) 305, 1140
SacI GAGCTC 1 cut(s) 577
SapI GCTCTTC 1 cut(s) 582
SaqAI TTAA 2 cut(s) 252, 279
Sau3AI GATC 7 cut(s) 340, 495, 802, 1012, 1051, 1069, 1279
Sau96I GGNCC 1 cut(s) 667
SchI GAGTC 5 cut(s) 365, 582, 1059, 1237, 1306
ScrFI CCNGG 2 cut(s) 411, 884
SduI GDGCHC 2 cut(s) 534, 577
SfaNI GCATC 1 cut(s) 1278
SfcI CTRYAG 3 cut(s) 171, 1008, 1155
Sfr274I CTCGAG 1 cut(s) 582
SinI GGWCC 1 cut(s) 667
SlaI CTCGAG 1 cut(s) 582
SmiMI CAYNNNNRTG 2 cut(s) 305, 1140
SmlI CTYRAG 3 cut(s) 582, 998, 1271
SmoI CTYRAG 3 cut(s) 582, 998, 1271
Sse9I AATT 9 cut(s) 16, 233, 323, 535, 547, 731, 862, 923, 1260
SsiI CCGC 1 cut(s) 851
SspI AATATT 1 cut(s) 97
SspMI CTAG 2 cut(s) 141, 707
SstI GAGCTC 1 cut(s) 577
StyD4I CCNGG 2 cut(s) 409, 882
StyI CCWWGG 1 cut(s) 202
TaaI ACNGT 5 cut(s) 374, 617, 796, 1100, 1139
TaqI TCGA 9 cut(s) 111, 267, 494, 583, 630, 1068, 1121, 1245, 1286
TaqII GACCGA 1 cut(s) 1005
TasI AATT 9 cut(s) 16, 233, 323, 535, 547, 731, 862, 923, 1260
TatI WGTACW 3 cut(s) 22, 661, 735
TauI GCSGC 1 cut(s) 854
TfiI GAWTC 4 cut(s) 125, 365, 601, 693
Tru1I TTAA 2 cut(s) 252, 279
Tru9I TTAA 2 cut(s) 252, 279
TscAI CASTG 2 cut(s) 763, 1170
TseFI GTSAC 3 cut(s) 403, 1094, 1161
Tsp45I GTSAC 3 cut(s) 403, 1094, 1161
TspDTI ATGAA 5 cut(s) 246, 336, 764, 1116, 1280
TspRI CASTG 2 cut(s) 763, 1170
VpaK11BI GGWCC 1 cut(s) 667
XagI CCTNNNNNAGG 1 cut(s) 1274
XapI RAATTY 2 cut(s) 16, 547
XhoI CTCGAG 1 cut(s) 582
XspI CTAG 2 cut(s) 141, 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.