Rorug02G0486500

Leucine-rich repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007873
Physical Location & Seq
Reverse (-)
61616488 .. 61619223
2736 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0486500.1

Sequence Viewer

Length: 1047 bp
ATGGAAGTAGCTTCGGAACCCACCAGAGTTCAAAGCCTAGCCCAAGCTGGTCTCAGCCAAGTTCCACCGCAGTACATTCAACCTCCCCAAAACCGACCCAACCACCCTTCATCTTCCTCCAACGTTCCCTCAATCAGCCTATTCGGACTCGACCCGACCCGCCGCGACTCGGTCCGGGCCTCCATCGCCGAAGCCTGCAGGGACTGGGGCGCCTTCCACGTCACCGACCACGGCGTCCCCGCCACTCTAGTCCGCGACATCAAGCGCGTGGGCCTCACCTTCTTCAACGAGTGCCCAGTCCAGGAGAAGCTCCGCTACGCCTGCGATCCCAGCTCCGCCGCGGCGGAGGGCTACGGCAGCCGGATGCTGGAGAAGGACGACACCGTTTTGGACTGGAGAGACTACTTCGACCACCACACTCTCCCCCTCACGCGCCGGAACCCCACGCGCTGGCCGGATTTCCCGCCGGATTACCGCCGGGTGGTTACGGAATACAGCGATCGAGTCGCTAAGCTGGCGAGGGAGCTGCTGGGGCTGATTTCCGAGAGCCTAGGGCTCAAAACGCAGCGTTTAGAGGAGGCGGTTGGGGATTTTTACCAGAACATTACGATTAGCTATTACCCGCCGTGTCCTCAGCCGGAGCTGACATTGGGCCTGCAGGCCCATTCGGATTTTGGGGCCATTACGCTGCTGGTCCAAGACGAGGTTGGTGGGCTTGAGGTGCTGAAAGACAATGAGTGGGTGCCTGTGAAGCCTTTGGGTGGTGATGCCATTGTTGTTCTTCTAGCTGACCAAACTGAGATCATGACCAACGGAAAGTACAAGAGTTCTCAACATAGAGCTATAACCAACTCCAATCGCCCACGGCTGTCAGTAGCTACTTTTCATGATCCTGCCAAGACAGTGAAAGTATCCCCTGCTTCAGAGCTCATCAGTCAATCCACTCCTCCCCTCTACCGGGAAGTTGTTTACGGAGATTATGTGTCATCATGGTACACAAAGGGTCCAGAAGGAAAACGGAATATTGATGCCCTCCTGCTTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_6 LRR_8

Protein Analysis

348

Amino Acids

38.82

Weight (kDa)

5.69

Isoelectric Point (pI)

51.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 42 - 153 1.5e-22 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 202 - 298 2.9e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 233
AccB1I GGYRCC 2 cut(s) 209, 742
AccB7I CCANNNNNTGG 1 cut(s) 450
AccII CGCG 6 cut(s) 165, 255, 267, 341, 433, 448
AclI AACGTT 1 cut(s) 123
AclWI GGATC 2 cut(s) 320, 884
AcoI YGGCCR 1 cut(s) 452
AcuI CTGAAG 1 cut(s) 906
AcyI GRCGYC 2 cut(s) 210, 234
AfaI GTAC 3 cut(s) 74, 821, 995
AfiI CCNNNNNNNGG 9 cut(s) 169, 301, 367, 450, 481, 703, 761, 957, 958
AgsI TTSAA 4 cut(s) 32, 80, 286, 1043
AjiI CACGTC 1 cut(s) 220
AjnI CCWGG 1 cut(s) 300
Alw21I GWGCWC 1 cut(s) 930
Alw26I GTCTC 2 cut(s) 56, 393
AlwI GGATC 2 cut(s) 320, 884
AlwNI CAGNNNCTG 1 cut(s) 204
AoxI GGCC 6 cut(s) 177, 271, 452, 652, 660, 678
ApeKI GCWGC 4 cut(s) 357, 526, 565, 688
AspA2I CCTAGG 1 cut(s) 550
AspLEI GCGC 4 cut(s) 212, 267, 435, 450
AspS9I GGNCC 8 cut(s) 172, 177, 271, 652, 661, 678, 694, 1004
AsuC2I CCSGG 3 cut(s) 176, 479, 959
AsuHPI GGTGA 3 cut(s) 214, 268, 776
AvaII GGWCC 3 cut(s) 172, 694, 1004
AvrII CCTAGG 1 cut(s) 550
BaeGI GKGCMC 1 cut(s) 296
BanI GGYRCC 2 cut(s) 209, 742
BanII GRGCYC 2 cut(s) 558, 930
BarI GAAGNNNNNNTAC 2 cut(s) 299, 331
Bbv12I GWGCWC 1 cut(s) 930
BbvCI CCTCAGC 1 cut(s) 633
BbvI GCAGC 4 cut(s) 369, 513, 577, 675
BccI CCATC 1 cut(s) 191
BceAI ACGGC 4 cut(s) 247, 370, 610, 881
BcgI CGANNNNNNTGC 2 cut(s) 508, 542
BciT130I CCWGG 1 cut(s) 302
BciVI GTATCC 1 cut(s) 922
BcnI CCSGG 3 cut(s) 176, 479, 959
BcoDI GTCTC 2 cut(s) 56, 393
BfaI CTAG 4 cut(s) 38, 248, 551, 785
BfmI CTRYAG 2 cut(s) 196, 656
BfoI RGCGCY 1 cut(s) 213
BfuI GTATCC 1 cut(s) 922
BisI GCNGC 7 cut(s) 163, 339, 342, 358, 527, 566, 689
BlnI CCTAGG 1 cut(s) 550
BlpI GCTNAGC 1 cut(s) 510
BlsI GCNGC 7 cut(s) 164, 340, 343, 359, 528, 567, 690
Bme1390I CCNGG 4 cut(s) 176, 302, 479, 959
Bme18I GGWCC 3 cut(s) 172, 694, 1004
BmgBI CACGTC 1 cut(s) 220
BmgT120I GGNCC 8 cut(s) 172, 177, 271, 652, 661, 678, 694, 1004
BmiI GGNNCC 6 cut(s) 18, 211, 440, 679, 744, 1005
BmrFI CCNGG 4 cut(s) 176, 302, 479, 959
BmrI ACTGGG 2 cut(s) 214, 290
BmsI GCATC 3 cut(s) 354, 757, 1018
BmuI ACTGGG 2 cut(s) 214, 290
BpmI CTGGAG 2 cut(s) 389, 415
Bpu10I CCTNAGC 1 cut(s) 633
Bpu1102I GCTNAGC 1 cut(s) 510
BpuEI CTTGAG 1 cut(s) 737
BpuMI CCSGG 3 cut(s) 176, 479, 959
BsaHI GRCGYC 2 cut(s) 210, 234
BsaI GGTCTC 1 cut(s) 56
BsaJI CCNNGG 4 cut(s) 229, 339, 550, 863
BsaXI ACNNNNNCTCC 2 cut(s) 966, 996
Bsc4I CCNNNNNNNGG 9 cut(s) 169, 301, 367, 450, 481, 703, 761, 957, 958
Bse1I ACTGG 3 cut(s) 209, 296, 398
BseBI CCWGG 1 cut(s) 302
BseDI CCNNGG 4 cut(s) 229, 339, 550, 863
BseGI GGATG 1 cut(s) 369
BseLI CCNNNNNNNGG 9 cut(s) 169, 301, 367, 450, 481, 703, 761, 957, 958
BseMII CTCAG 3 cut(s) 67, 647, 789
BseNI ACTGG 3 cut(s) 209, 296, 398
BseRI GAGGAG 2 cut(s) 590, 936
BseSI GKGCMC 1 cut(s) 296
BseXI GCAGC 4 cut(s) 369, 513, 577, 675
BseYI CCCAGC 2 cut(s) 329, 529
Bsh1236I CGCG 6 cut(s) 165, 255, 267, 341, 433, 448
Bsh1285I CGRYCG 1 cut(s) 502
BshFI GGCC 6 cut(s) 179, 273, 454, 654, 662, 680
BshNI GGYRCC 2 cut(s) 209, 742
BsiEI CGRYCG 1 cut(s) 502
BsiHKAI GWGCWC 1 cut(s) 930
BsiSI CCGG 8 cut(s) 175, 361, 436, 455, 467, 478, 638, 958
BslFI GGGAC 2 cut(s) 215, 221
BslI CCNNNNNNNGG 9 cut(s) 169, 301, 367, 450, 481, 703, 761, 957, 958
BsmAI GTCTC 2 cut(s) 56, 393
BsmFI GGGAC 2 cut(s) 215, 221
BsnI GGCC 6 cut(s) 179, 273, 454, 654, 662, 680
Bso31I GGTCTC 1 cut(s) 56
Bsp1286I GDGCHC 3 cut(s) 296, 558, 930
Bsp143I GATC 4 cut(s) 325, 499, 801, 889
Bsp1720I GCTNAGC 1 cut(s) 510
BspANI GGCC 6 cut(s) 179, 273, 454, 654, 662, 680
BspCNI CTCAG 3 cut(s) 66, 646, 790
BspFNI CGCG 6 cut(s) 165, 255, 267, 341, 433, 448
BspHI TCATGA 2 cut(s) 804, 886
BspLI GGNNCC 6 cut(s) 18, 211, 440, 679, 744, 1005
BspMAI CTGCAG 2 cut(s) 200, 660
BspPI GGATC 2 cut(s) 320, 884
BspT107I GGYRCC 2 cut(s) 209, 742
BspTNI GGTCTC 1 cut(s) 56
BsrI ACTGG 3 cut(s) 209, 296, 398
BssECI CCNNGG 4 cut(s) 229, 339, 550, 863
BssMI GATC 4 cut(s) 325, 499, 801, 889
BssNI GRCGYC 2 cut(s) 210, 234
BssT1I CCWWGG 1 cut(s) 550
Bst2UI CCWGG 1 cut(s) 302
Bst4CI ACNGT 2 cut(s) 385, 904
BstACI GRCGYC 2 cut(s) 210, 234
BstC8I GCNNGC 6 cut(s) 196, 322, 452, 516, 656, 660
BstDEI CTNAG 4 cut(s) 53, 510, 633, 798
BstDSI CCRYGG 3 cut(s) 229, 339, 863
BstF5I GGATG 1 cut(s) 369
BstFNI CGCG 6 cut(s) 165, 255, 267, 341, 433, 448
BstH2I RGCGCY 1 cut(s) 213
BstHHI GCGC 4 cut(s) 212, 267, 435, 450
BstKTI GATC 4 cut(s) 328, 502, 804, 892
BstMAI GTCTC 2 cut(s) 56, 393
BstMBI GATC 4 cut(s) 325, 499, 801, 889
BstMCI CGRYCG 1 cut(s) 502
BstMWI GCNNNNNNNGC 9 cut(s) 185, 321, 330, 357, 515, 532, 562, 721, 751
BstNI CCWGG 1 cut(s) 302
BstSCI CCNGG 4 cut(s) 174, 300, 477, 957
BstSFI CTRYAG 2 cut(s) 196, 656
BstSLI GKGCMC 1 cut(s) 296
BstUI CGCG 6 cut(s) 165, 255, 267, 341, 433, 448
BstV1I GCAGC 4 cut(s) 369, 513, 577, 675
BsuI GTATCC 1 cut(s) 922
BsuRI GGCC 6 cut(s) 179, 273, 454, 654, 662, 680
BtgI CCRYGG 3 cut(s) 229, 339, 863
BtgZI GCGATG 1 cut(s) 169
BtrI CACGTC 1 cut(s) 220
BtsCI GGATG 1 cut(s) 369
BtsIMutI CAGTG 1 cut(s) 909
Cac8I GCNNGC 6 cut(s) 196, 322, 452, 516, 656, 660
CaiI CAGNNNCTG 1 cut(s) 204
CciI TCATGA 2 cut(s) 804, 886
CfoI GCGC 4 cut(s) 212, 267, 435, 450
Cfr13I GGNCC 8 cut(s) 172, 177, 271, 652, 661, 678, 694, 1004
Cfr42I CCGCGG 1 cut(s) 342
CpoI CGGWCCG 1 cut(s) 172
CseI GACGC 1 cut(s) 223
Csp6I GTAC 3 cut(s) 73, 820, 994
CspI CGGWCCG 1 cut(s) 172
CviAII CATG 3 cut(s) 805, 887, 990
CviQI GTAC 3 cut(s) 73, 820, 994
DdeI CTNAG 4 cut(s) 53, 510, 633, 798
DinI GGCGCC 1 cut(s) 211
DpnI GATC 4 cut(s) 327, 501, 803, 891
DpnII GATC 4 cut(s) 325, 499, 801, 889
DrdI GACNNNNNNGTC 1 cut(s) 233
DseDI GACNNNNNNGTC 1 cut(s) 233
EaeI YGGCCR 1 cut(s) 452
EciI GGCGGA 2 cut(s) 325, 359
Ecl136II GAGCTC 1 cut(s) 928
Eco130I CCWWGG 1 cut(s) 550
Eco24I GRGCYC 2 cut(s) 558, 930
Eco31I GGTCTC 1 cut(s) 56
Eco47I GGWCC 3 cut(s) 172, 694, 1004
Eco53kI GAGCTC 1 cut(s) 928
Eco57I CTGAAG 1 cut(s) 906
EcoICRI GAGCTC 1 cut(s) 928
EcoRII CCWGG 1 cut(s) 300
EcoT14I CCWWGG 1 cut(s) 550
EcoT38I GRGCYC 2 cut(s) 558, 930
EgeI GGCGCC 1 cut(s) 211
EheI GGCGCC 1 cut(s) 211
ErhI CCWWGG 1 cut(s) 550
FaeI CATG 3 cut(s) 808, 890, 993
FaiI YATR 6 cut(s) 806, 837, 845, 888, 981, 991
FaqI GGGAC 2 cut(s) 215, 221
FatI CATG 3 cut(s) 804, 886, 989
FauI CCCGC 4 cut(s) 167, 247, 471, 630
Fnu4HI GCNGC 7 cut(s) 163, 339, 342, 358, 527, 566, 689
FokI GGATG 1 cut(s) 376
FriOI GRGCYC 2 cut(s) 558, 930
Fsp4HI GCNGC 7 cut(s) 163, 339, 342, 358, 527, 566, 689
FspBI CTAG 4 cut(s) 38, 248, 551, 785
GlaI GCGC 4 cut(s) 211, 266, 434, 449
GluI GCNGC 7 cut(s) 163, 339, 342, 358, 527, 566, 689
GsaI CCCAGC 2 cut(s) 333, 533
GsuI CTGGAG 2 cut(s) 389, 415
HaeII RGCGCY 1 cut(s) 213
HaeIII GGCC 6 cut(s) 179, 273, 454, 654, 662, 680
HapII CCGG 8 cut(s) 175, 361, 436, 455, 467, 478, 638, 958
HgaI GACGC 1 cut(s) 223
HhaI GCGC 4 cut(s) 212, 267, 435, 450
Hin1I GRCGYC 2 cut(s) 210, 234
Hin1II CATG 3 cut(s) 808, 890, 993
Hin6I GCGC 4 cut(s) 210, 265, 433, 448
HinP1I GCGC 4 cut(s) 210, 265, 433, 448
HinfI GANTC 3 cut(s) 147, 167, 504
HpaII CCGG 8 cut(s) 175, 361, 436, 455, 467, 478, 638, 958
HphI GGTGA 3 cut(s) 214, 268, 776
Hpy166II GTNNAC 2 cut(s) 970, 996
Hpy188I TCNGA 5 cut(s) 16, 146, 544, 670, 925
Hpy188III TCNNGA 3 cut(s) 805, 887, 1007
Hpy8I GTNNAC 2 cut(s) 970, 996
HpyAV CCTTC 5 cut(s) 117, 223, 289, 367, 1004
HpyCH4III ACNGT 2 cut(s) 385, 904
HpyCH4IV ACGT 2 cut(s) 123, 219
HpyCH4V TGCA 2 cut(s) 198, 658
HpyF10VI GCNNNNNNNGC 9 cut(s) 185, 321, 330, 357, 515, 532, 562, 721, 751
HpyF3I CTNAG 4 cut(s) 53, 510, 633, 798
HpySE526I ACGT 2 cut(s) 123, 219
Hsp92I GRCGYC 2 cut(s) 210, 234
Hsp92II CATG 3 cut(s) 808, 890, 993
HspAI GCGC 4 cut(s) 210, 265, 433, 448
KasI GGCGCC 1 cut(s) 209
KspI CCGCGG 1 cut(s) 342
Kzo9I GATC 4 cut(s) 325, 499, 801, 889
LmnI GCTCC 4 cut(s) 315, 338, 523, 640
Lsp1109I GCAGC 4 cut(s) 369, 513, 577, 675
LweI GCATC 3 cut(s) 354, 757, 1018
MaeI CTAG 4 cut(s) 38, 248, 551, 785
MaeII ACGT 2 cut(s) 123, 219
MaeIII GTNAC 2 cut(s) 220, 484
MalI GATC 4 cut(s) 327, 501, 803, 891
MboI GATC 4 cut(s) 325, 499, 801, 889
MboII GAAGA 3 cut(s) 105, 274, 773
MhlI GDGCHC 3 cut(s) 296, 558, 930
Mly113I GGCGCC 1 cut(s) 210
MlyI GAGTC 3 cut(s) 141, 161, 513
MmeI TCCRAC 1 cut(s) 144
MspA1I CMGCKG 1 cut(s) 341
MspI CCGG 8 cut(s) 175, 361, 436, 455, 467, 478, 638, 958
MspR9I CCNGG 4 cut(s) 176, 302, 479, 959
MvaI CCWGG 1 cut(s) 302
MvnI CGCG 6 cut(s) 165, 255, 267, 341, 433, 448
MwoI GCNNNNNNNGC 9 cut(s) 185, 321, 330, 357, 515, 532, 562, 721, 751
NarI GGCGCC 1 cut(s) 210
NciI CCSGG 3 cut(s) 176, 479, 959
NdeII GATC 4 cut(s) 325, 499, 801, 889
NlaIII CATG 3 cut(s) 808, 890, 993
NlaIV GGNNCC 6 cut(s) 18, 211, 440, 679, 744, 1005
NmuCI GTSAC 1 cut(s) 220
PagI TCATGA 2 cut(s) 804, 886
PflFI GACNNNGTC 1 cut(s) 170
PflMI CCANNNNNTGG 1 cut(s) 450
PfoI TCCNGGA 1 cut(s) 300
PkrI GCNGC 7 cut(s) 164, 340, 343, 359, 528, 567, 690
Ple19I CGATCG 1 cut(s) 502
PleI GAGTC 3 cut(s) 141, 161, 512
PluTI GGCGCC 1 cut(s) 213
PpsI GAGTC 3 cut(s) 141, 161, 512
Psp124BI GAGCTC 1 cut(s) 930
Psp1406I AACGTT 1 cut(s) 123
Psp6I CCWGG 1 cut(s) 300
PspFI CCCAGC 2 cut(s) 329, 529
PspGI CCWGG 1 cut(s) 300
PspN4I GGNNCC 6 cut(s) 18, 211, 440, 679, 744, 1005
PspPI GGNCC 8 cut(s) 172, 177, 271, 652, 661, 678, 694, 1004
PstI CTGCAG 2 cut(s) 200, 660
PstNI CAGNNNCTG 1 cut(s) 204
PsyI GACNNNGTC 1 cut(s) 170
PvuI CGATCG 1 cut(s) 502
RsaI GTAC 3 cut(s) 74, 821, 995
RsaNI GTAC 3 cut(s) 73, 820, 994
Rsr2I CGGWCCG 1 cut(s) 172
RsrII CGGWCCG 1 cut(s) 172
SacI GAGCTC 1 cut(s) 930
SacII CCGCGG 1 cut(s) 342
SatI GCNGC 7 cut(s) 163, 339, 342, 358, 527, 566, 689
Sau3AI GATC 4 cut(s) 325, 499, 801, 889
Sau96I GGNCC 8 cut(s) 172, 177, 271, 652, 661, 678, 694, 1004
SbfI CCTGCAGG 2 cut(s) 200, 660
SchI GAGTC 3 cut(s) 141, 161, 513
ScrFI CCNGG 4 cut(s) 176, 302, 479, 959
SdaI CCTGCAGG 2 cut(s) 200, 660
SduI GDGCHC 3 cut(s) 296, 558, 930
SfaNI GCATC 3 cut(s) 354, 757, 1018
SfcI CTRYAG 2 cut(s) 196, 656
SfoI GGCGCC 1 cut(s) 211
Sfr303I CCGCGG 1 cut(s) 342
SgrBI CCGCGG 1 cut(s) 342
SinI GGWCC 3 cut(s) 172, 694, 1004
SmlI CTYRAG 1 cut(s) 716
SmoI CTYRAG 1 cut(s) 716
Sse8387I CCTGCAGG 2 cut(s) 200, 660
SspDI GGCGCC 1 cut(s) 209
SspI AATATT 1 cut(s) 1024
SspMI CTAG 4 cut(s) 38, 248, 551, 785
SstI GAGCTC 1 cut(s) 930
StyD4I CCNGG 4 cut(s) 174, 300, 477, 957
StyI CCWWGG 1 cut(s) 550
TaaI ACNGT 2 cut(s) 385, 904
TaiI ACGT 2 cut(s) 126, 222
TaqI TCGA 3 cut(s) 150, 408, 502
TaqII GACCGA 1 cut(s) 160
TatI WGTACW 2 cut(s) 72, 819
TauI GCSGC 3 cut(s) 165, 341, 344
TscAI CASTG 1 cut(s) 909
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 4 cut(s) 357, 526, 565, 688
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 2 cut(s) 99, 875
TspGWI ACGGA 4 cut(s) 503, 828, 987, 1033
TspRI CASTG 1 cut(s) 909
Tth111I GACNNNGTC 1 cut(s) 170
Van91I CCANNNNNTGG 1 cut(s) 450
VpaK11BI GGWCC 3 cut(s) 172, 694, 1004
XcmI CCANNNNNNNNNTGG 3 cut(s) 671, 688, 704
XmaJI CCTAGG 1 cut(s) 550
XspI CTAG 4 cut(s) 38, 248, 551, 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.