MD09G1113900.v1.1

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0015945
Physical Location & Seq
Forward (+)
8654207 .. 8657193
2987 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1113900.v1.1.491

Sequence Viewer

Length: 1623 bp
ATGGTCGACTGGTTCGTCGCGTCAAACACGTGGGTCCCGCCATCTCGATCCGCACGTTATTCCGCTAAAGTCATAAAACCCCCTCACCTCCCCCCCCACTCCTCACTGTCACTCCCCTCCGCCGTACAAAATTACAAAGAAAAGCCAGAAGAGGAAAGAAGAAGCTCTCCACCTTTCACGATGCTCTACTTCTCCAAGAACCAGAGCATGGAACGCCATGTCATCGCCGTCCTGCTCCTCCTTTCTGTCTTTTCATCTCTAGCGGATGCTGCCTCGGTCGGAGTGAACTATGGTAGAATCGCCGACAACCTGCCGTCGGCGTACAAGGTGGTGCAACTTCTGAAGTCCCAAGGATTGGAGCGGGTCAAGGTCTTCGACTCCGACCCAGCCGTGCTCCGGGCCCTAGCTGGATCCGACATCAAGGTCACCGTCGACCTCCCCAACGAGCTCCTCTCCTCCGCCGCCAAGTCTCAGTCTTTCGCTACCAACTGGGTCCAGCGAAACGTCGTCGCTTACCACCCCAACACCCAGATCGAAGCTATCGGCGTCGGCAATGAGGTCTTCGTTGACACCCACCACACCACCAAGTTCCTCATCTCCGCTATGAAAAACATCCACACCGCCCTCGTCCACTTCAAGCTCGAGTCATCCATCAAAGTCTCCTCCCCCATTGCCCTCAGCGCCCTGCAAAACTCCTACCCGTCTTCCGCCGGCTCATTCCGTCCCGAACTCGTTGAACCCGTTTTCAAGCCCATGCTAGAGTTCCTTCGTCAAACCGGGTCCTATCTCATGGTGAACTGCTACCCCTACTTCGCTTACGAGTCAAACTCTGACGTCATCCCCTTGGACTATGCCCTCTTCCGTGAAAACCCTGGAGTCGTGGACGCCGGCAACGGCCTCCGTTATTTCAACCTCTTCGACGCCCAGATCGACGCCGTTTTCGCCGCCATGTCGGCGTTAAAGTACGACGACATCAACATGGTTGTGTCGGAAACGGGCTGGCCGTCCAAAGGCGACTCGGTTGAAGTAGGCGCGAGTGTGGAGAATGCAGCCGCCTACAACGGCAACCTGGTCCGTCGGATATTGACCGGCGGGGGGACCCCCTTGAGGCCCAAAGCAGATCTGACCGTCTATCTCTTCGCTCTATTCAACGAGGACAAGAAATTCGGTCCCACATCAGAGAGAAACTACGGGCTCTTCTTCCCCAACGAGCGGAAGGTGTACGACATACCGTTTACCGTCGAAGGGCTAAAGAACTACCACGACCGCCGGTCGCCGGTATCCGGAAACCAGCAGGTGACTGCTCCGGTCAACGGGGACGTGTCGAAGAGCTTGACCGGGAGCACGTGGTGCGTGGCGAATGGGGAGGCCGGGAAGGAGAAGCTGCAGGCGGGACTAGACTACGCTTGCGGTGAGGGCGGGGCGGACTGCCATCAGATCCAGCCGGGGTCCGCGTGTTACGATCCCAACACCCTGGAGGCCCACGCCTCATACGCTTTCAACAGTTACTACCAAAAGAAGGCACGCGGAGTCGGCACGTGCTATTTTGGCGGGGCGGCCTACGTCGTCTCTCAACCACCCAAGTTTGGGAAATGTGAGCTGCCGACTGGGTACGGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_17 X8

Protein Analysis

541

Amino Acids

59.06

Weight (kDa)

6.84

Isoelectric Point (pI)

37.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 93 - 412 4.1e-88 Glycosyl hydrolases family 17
X8 PF07983 449 - 520 3e-22 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015945)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G05790
fragaria_vesca FvH4_6g42190
malus_domestica MD09G1113900.v1.1 MD17G1104000.v1.1
prunus_persica Prupe.3G210700_v2.0.a1
pyrus_communis pycom09g03790
rosa_chinensis RchiOBHm_Chr2g0158111
rosa_laevigata RLG00000021060
rosa_multiflora Rmu_sc0005982.1_g000010
rosa_roxburghii Rroxscaffold_2G00091660
rosa_samantha Rh2AG532100 Rh2BG546200 Rh2CG516000 Rh2DG554700
rosa_wichuraiana Rw2G043990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1285
AasI GACNNNNNNGTC 2 cut(s) 14, 422
AatII GACGTC 1 cut(s) 837
Acc36I ACCTGC 2 cut(s) 318, 1285
AccB7I CCANNNNNTGG 2 cut(s) 208, 355
AccBSI CCGCTC 2 cut(s) 361, 1213
AccI GTMKAC 2 cut(s) 6, 432
AccII CGCG 4 cut(s) 20, 1034, 1454, 1527
AccIII TCCGGA 1 cut(s) 1283
AclWI GGATC 5 cut(s) 42, 405, 418, 1432, 1457
AcoI YGGCCR 1 cut(s) 1001
AcsI RAATTY 1 cut(s) 1163
AcuI CTGAAG 1 cut(s) 362
AcvI CACGTG 3 cut(s) 30, 1347, 1539
AcyI GRCGYC 5 cut(s) 546, 834, 885, 921, 933
AdeI CACNNNGTG 1 cut(s) 1350
AfaI GTAC 5 cut(s) 126, 323, 965, 1223, 1613
AflIII ACRYGT 2 cut(s) 27, 1320
AgsI TTSAA 7 cut(s) 637, 737, 748, 910, 1025, 1150, 1501
AhdI GACNNNNNGTC 1 cut(s) 1270
AjiI CACGTC 1 cut(s) 1321
AjnI CCWGG 3 cut(s) 871, 1068, 1473
AluBI AGCT 8 cut(s) 165, 407, 448, 539, 640, 1332, 1384, 1600
AluI AGCT 8 cut(s) 165, 407, 448, 539, 640, 1332, 1384, 1600
Alw21I GWGCWC 3 cut(s) 396, 450, 1346
Alw26I GTCTC 3 cut(s) 474, 664, 1573
AlwI GGATC 5 cut(s) 42, 405, 418, 1432, 1457
Ama87I CYCGRG 1 cut(s) 641
Aor13HI TCCGGA 1 cut(s) 1283
AoxI GGCC 7 cut(s) 399, 895, 1001, 1109, 1368, 1479, 1557
ApaI GGGCCC 1 cut(s) 403
ApeKI GCWGC 4 cut(s) 269, 1049, 1384, 1600
ApoI RAATTY 1 cut(s) 1163
ArsI GACNNNNNNTTYG 4 cut(s) 923, 955, 1148, 1180
AspLEI GCGC 2 cut(s) 683, 1034
AsuC2I CCSGG 5 cut(s) 398, 778, 1339, 1372, 1446
AsuHPI GGTGA 5 cut(s) 77, 418, 805, 1309, 1424
AvaI CYCGRG 1 cut(s) 641
AvaII GGWCC 7 cut(s) 34, 493, 780, 1072, 1098, 1169, 1449
BaeGI GKGCMC 1 cut(s) 403
BamHI GGATCC 1 cut(s) 410
BanII GRGCYC 3 cut(s) 403, 450, 1197
BbrPI CACGTG 3 cut(s) 30, 1347, 1539
BbsI GAAGAC 3 cut(s) 364, 553, 696
Bbv12I GWGCWC 3 cut(s) 396, 450, 1346
BbvCI CCTCAGC 1 cut(s) 677
BbvI GCAGC 4 cut(s) 256, 1061, 1371, 1587
BccI CCATC 3 cut(s) 49, 659, 1440
BceAI ACGGC 8 cut(s) 107, 212, 298, 374, 910, 920, 988, 1078
BciT130I CCWGG 3 cut(s) 873, 1070, 1475
BciVI GTATCC 1 cut(s) 1291
BcnI CCSGG 5 cut(s) 398, 778, 1339, 1372, 1446
BcoDI GTCTC 3 cut(s) 474, 664, 1573
BfaI CTAG 4 cut(s) 260, 404, 758, 1397
BfmI CTRYAG 1 cut(s) 1385
BfoI RGCGCY 1 cut(s) 684
BfuAI ACCTGC 2 cut(s) 318, 1285
BfuI GTATCC 1 cut(s) 1291
BglI GCCNNNNNGGC 1 cut(s) 953
BglII AGATCT 1 cut(s) 1120
BisI GCNGC 8 cut(s) 270, 462, 945, 1050, 1053, 1385, 1557, 1601
BlsI GCNGC 8 cut(s) 271, 463, 946, 1051, 1054, 1386, 1558, 1602
Bme1390I CCNGG 8 cut(s) 398, 778, 873, 1070, 1339, 1372, 1446, 1475
Bme18I GGWCC 7 cut(s) 34, 493, 780, 1072, 1098, 1169, 1449
BmeRI GACNNNNNGTC 1 cut(s) 1270
BmeT110I CYCGRG 1 cut(s) 641
BmgBI CACGTC 1 cut(s) 1321
BmrFI CCNGG 8 cut(s) 398, 778, 873, 1070, 1339, 1372, 1446, 1475
BmrI ACTGGG 2 cut(s) 499, 1617
BmsI GCATC 2 cut(s) 171, 256
BmuI ACTGGG 2 cut(s) 499, 1617
BpiI GAAGAC 3 cut(s) 364, 553, 696
BplI GAGNNNNNCTC 4 cut(s) 437, 469, 812, 844
BpmI CTGGAG 2 cut(s) 894, 1496
Bpu10I CCTNAGC 1 cut(s) 677
BpuEI CTTGAG 1 cut(s) 1126
BpuMI CCSGG 5 cut(s) 398, 778, 1339, 1372, 1446
BsaAI YACGTR 3 cut(s) 30, 1347, 1539
BsaHI GRCGYC 5 cut(s) 546, 834, 885, 921, 933
BsaJI CCNNGG 6 cut(s) 273, 349, 843, 871, 1445, 1473
BsaWI WCCGGW 2 cut(s) 1283, 1306
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bse118I RCCGGY 5 cut(s) 710, 887, 1088, 1269, 1276
Bse1I ACTGG 3 cut(s) 14, 494, 1612
Bse3DI GCAATG 2 cut(s) 559, 669
BseAI TCCGGA 1 cut(s) 1283
BseBI CCWGG 3 cut(s) 873, 1070, 1475
BseDI CCNNGG 6 cut(s) 273, 349, 843, 871, 1445, 1473
BseGI GGATG 4 cut(s) 271, 612, 647, 837
BseMI GCAATG 2 cut(s) 559, 669
BseMII CTCAG 2 cut(s) 485, 691
BseNI ACTGG 3 cut(s) 14, 494, 1612
BseRI GAGGAG 5 cut(s) 91, 227, 440, 445, 652
BseSI GKGCMC 1 cut(s) 403
BseXI GCAGC 4 cut(s) 256, 1061, 1371, 1587
BseYI CCCAGC 1 cut(s) 385
Bsh1236I CGCG 4 cut(s) 20, 1034, 1454, 1527
Bsh1285I CGRYCG 3 cut(s) 279, 1267, 1274
BshFI GGCC 7 cut(s) 401, 897, 1003, 1111, 1370, 1481, 1559
BsiEI CGRYCG 3 cut(s) 279, 1267, 1274
BsiHKAI GWGCWC 3 cut(s) 396, 450, 1346
BsiHKCI CYCGRG 1 cut(s) 641
BslFI GGGAC 7 cut(s) 20, 331, 708, 1111, 1155, 1331, 1407
BsmAI GTCTC 3 cut(s) 474, 664, 1573
BsmBI CGTCTC 1 cut(s) 1573
BsmFI GGGAC 7 cut(s) 20, 331, 708, 1111, 1155, 1331, 1407
BsmI GAATGC 1 cut(s) 1051
BsnI GGCC 7 cut(s) 401, 897, 1003, 1111, 1370, 1481, 1559
BsoBI CYCGRG 1 cut(s) 641
Bsp120I GGGCCC 1 cut(s) 399
Bsp1286I GDGCHC 5 cut(s) 396, 403, 450, 1197, 1346
Bsp13I TCCGGA 1 cut(s) 1283
Bsp143I GATC 7 cut(s) 47, 410, 531, 927, 1120, 1437, 1462
BspANI GGCC 7 cut(s) 401, 897, 1003, 1111, 1370, 1481, 1559
BspCNI CTCAG 2 cut(s) 484, 690
BspEI TCCGGA 1 cut(s) 1283
BspFNI CGCG 4 cut(s) 20, 1034, 1454, 1527
BspMAI CTGCAG 1 cut(s) 1389
BspMI ACCTGC 2 cut(s) 318, 1285
BspPI GGATC 5 cut(s) 42, 405, 418, 1432, 1457
BspQI GCTCTTC 2 cut(s) 1202, 1322
BsrBI CCGCTC 2 cut(s) 361, 1213
BsrDI GCAATG 2 cut(s) 559, 669
BsrFI RCCGGY 5 cut(s) 710, 887, 1088, 1269, 1276
BsrI ACTGG 3 cut(s) 14, 494, 1612
BssAI RCCGGY 5 cut(s) 710, 887, 1088, 1269, 1276
BssECI CCNNGG 6 cut(s) 273, 349, 843, 871, 1445, 1473
BssMI GATC 7 cut(s) 47, 410, 531, 927, 1120, 1437, 1462
BssNI GRCGYC 5 cut(s) 546, 834, 885, 921, 933
BssT1I CCWWGG 2 cut(s) 349, 843
Bst2UI CCWGG 3 cut(s) 873, 1070, 1475
Bst4CI ACNGT 6 cut(s) 108, 430, 1129, 1233, 1240, 1505
Bst6I CTCTTC 6 cut(s) 144, 863, 920, 1142, 1202, 1322
BstACI GRCGYC 5 cut(s) 546, 834, 885, 921, 933
BstAPI GCANNNNNTGC 1 cut(s) 1350
BstBAI YACGTR 3 cut(s) 30, 1347, 1539
BstC8I GCNNGC 6 cut(s) 712, 889, 1001, 1389, 1408, 1525
BstDEI CTNAG 2 cut(s) 471, 677
BstEII GGTNACC 1 cut(s) 424
BstF5I GGATG 4 cut(s) 271, 612, 647, 837
BstFNI CGCG 4 cut(s) 20, 1034, 1454, 1527
BstH2I RGCGCY 1 cut(s) 684
BstHHI GCGC 2 cut(s) 683, 1034
BstKTI GATC 7 cut(s) 50, 413, 534, 930, 1123, 1440, 1465
BstMAI GTCTC 3 cut(s) 474, 664, 1573
BstMBI GATC 7 cut(s) 47, 410, 531, 927, 1120, 1437, 1462
BstMCI CGRYCG 3 cut(s) 279, 1267, 1274
BstNI CCWGG 3 cut(s) 873, 1070, 1475
BstPI GGTNACC 1 cut(s) 424
BstSCI CCNGG 8 cut(s) 396, 776, 871, 1068, 1337, 1370, 1444, 1473
BstSFI CTRYAG 1 cut(s) 1385
BstSLI GKGCMC 1 cut(s) 403
BstUI CGCG 4 cut(s) 20, 1034, 1454, 1527
BstV1I GCAGC 4 cut(s) 256, 1061, 1371, 1587
BstV2I GAAGAC 3 cut(s) 364, 553, 696
BstX2I RGATCY 3 cut(s) 410, 1120, 1437
BstXI CCANNNNNNTGG 1 cut(s) 1474
BstYI RGATCY 3 cut(s) 410, 1120, 1437
BsuI GTATCC 1 cut(s) 1291
BsuRI GGCC 7 cut(s) 401, 897, 1003, 1111, 1370, 1481, 1559
BtgZI GCGATG 1 cut(s) 208
BtrI CACGTC 1 cut(s) 1321
BtsCI GGATG 4 cut(s) 271, 612, 647, 837
BtsIMutI CAGTG 1 cut(s) 104
BveI ACCTGC 2 cut(s) 318, 1285
Cac8I GCNNGC 6 cut(s) 712, 889, 1001, 1389, 1408, 1525
CfoI GCGC 2 cut(s) 683, 1034
Cfr10I RCCGGY 5 cut(s) 710, 887, 1088, 1269, 1276
CseI GACGC 5 cut(s) 9, 535, 893, 929, 941
CsiI ACCWGGT 1 cut(s) 1068
Csp6I GTAC 5 cut(s) 125, 322, 964, 1222, 1612
CviAII CATG 6 cut(s) 208, 218, 754, 790, 949, 979
CviQI GTAC 5 cut(s) 125, 322, 964, 1222, 1612
DdeI CTNAG 2 cut(s) 471, 677
DpnI GATC 7 cut(s) 49, 412, 533, 929, 1122, 1439, 1464
DpnII GATC 7 cut(s) 47, 410, 531, 927, 1120, 1437, 1462
DraIII CACNNNGTG 1 cut(s) 1350
DrdI GACNNNNNNGTC 2 cut(s) 14, 422
DriI GACNNNNNGTC 1 cut(s) 1270
DseDI GACNNNNNNGTC 2 cut(s) 14, 422
EaeI YGGCCR 1 cut(s) 1001
Eam1104I CTCTTC 6 cut(s) 144, 863, 920, 1142, 1202, 1322
Eam1105I GACNNNNNGTC 1 cut(s) 1270
EarI CTCTTC 6 cut(s) 144, 863, 920, 1142, 1202, 1322
EciI GGCGGA 4 cut(s) 109, 448, 697, 1439
Ecl136II GAGCTC 1 cut(s) 448
Eco130I CCWWGG 2 cut(s) 349, 843
Eco24I GRGCYC 3 cut(s) 403, 450, 1197
Eco47I GGWCC 7 cut(s) 34, 493, 780, 1072, 1098, 1169, 1449
Eco53kI GAGCTC 1 cut(s) 448
Eco57I CTGAAG 1 cut(s) 362
Eco72I CACGTG 3 cut(s) 30, 1347, 1539
Eco88I CYCGRG 1 cut(s) 641
Eco91I GGTNACC 1 cut(s) 424
EcoICRI GAGCTC 1 cut(s) 448
EcoO109I RGGNCCY 4 cut(s) 34, 400, 780, 1098
EcoO65I GGTNACC 1 cut(s) 424
EcoRII CCWGG 3 cut(s) 871, 1068, 1473
EcoT14I CCWWGG 2 cut(s) 349, 843
EcoT38I GRGCYC 3 cut(s) 403, 450, 1197
ErhI CCWWGG 2 cut(s) 349, 843
Esp3I CGTCTC 1 cut(s) 1573
FaeI CATG 6 cut(s) 211, 221, 757, 793, 952, 982
FaqI GGGAC 7 cut(s) 20, 331, 708, 1111, 1155, 1331, 1407
FatI CATG 6 cut(s) 207, 217, 753, 789, 948, 978
FauI CCCGC 6 cut(s) 45, 354, 1085, 1384, 1412, 1544
FblI GTMKAC 2 cut(s) 6, 432
Fnu4HI GCNGC 8 cut(s) 270, 462, 945, 1050, 1053, 1385, 1557, 1601
FokI GGATG 4 cut(s) 278, 599, 634, 824
FriOI GRGCYC 3 cut(s) 403, 450, 1197
Fsp4HI GCNGC 8 cut(s) 270, 462, 945, 1050, 1053, 1385, 1557, 1601
FspBI CTAG 4 cut(s) 260, 404, 758, 1397
GlaI GCGC 2 cut(s) 682, 1033
GluI GCNGC 8 cut(s) 270, 462, 945, 1050, 1053, 1385, 1557, 1601
GsaI CCCAGC 1 cut(s) 389
GsuI CTGGAG 2 cut(s) 894, 1496
HaeII RGCGCY 1 cut(s) 684
HaeIII GGCC 7 cut(s) 401, 897, 1003, 1111, 1370, 1481, 1559
HgaI GACGC 5 cut(s) 9, 535, 893, 929, 941
HhaI GCGC 2 cut(s) 683, 1034
Hin1I GRCGYC 5 cut(s) 546, 834, 885, 921, 933
Hin1II CATG 6 cut(s) 211, 221, 757, 793, 952, 982
Hin6I GCGC 2 cut(s) 681, 1032
HinP1I GCGC 2 cut(s) 681, 1032
HincII GTYRAC 4 cut(s) 7, 433, 568, 1312
HindII GTYRAC 4 cut(s) 7, 433, 568, 1312
HinfI GANTC 7 cut(s) 297, 377, 644, 821, 876, 1016, 1530
HphI GGTGA 5 cut(s) 77, 418, 805, 1309, 1424
Hpy188III TCNNGA 4 cut(s) 45, 178, 725, 1284
HpyAV CCTTC 5 cut(s) 776, 1210, 1238, 1369, 1513
HpyCH4III ACNGT 6 cut(s) 108, 430, 1129, 1233, 1240, 1505
HpyCH4IV ACGT 8 cut(s) 29, 55, 504, 834, 1320, 1346, 1538, 1563
HpyCH4V TGCA 4 cut(s) 334, 688, 1049, 1387
HpyF3I CTNAG 2 cut(s) 471, 677
HpySE526I ACGT 8 cut(s) 29, 55, 504, 834, 1320, 1346, 1538, 1563
Hsp92I GRCGYC 5 cut(s) 546, 834, 885, 921, 933
Hsp92II CATG 6 cut(s) 211, 221, 757, 793, 952, 982
HspAI GCGC 2 cut(s) 681, 1032
KflI GGGWCCC 2 cut(s) 34, 1098
Kpn2I TCCGGA 1 cut(s) 1283
KroI GCCGGC 2 cut(s) 710, 887
KroNI GCCGGC 2 cut(s) 712, 889
Kzo9I GATC 7 cut(s) 47, 410, 531, 927, 1120, 1437, 1462
LguI GCTCTTC 2 cut(s) 1202, 1322
LmnI GCTCC 6 cut(s) 240, 358, 399, 453, 1309, 1341
Lsp1109I GCAGC 4 cut(s) 256, 1061, 1371, 1587
LweI GCATC 2 cut(s) 171, 256
MabI ACCWGGT 1 cut(s) 1068
MaeI CTAG 4 cut(s) 260, 404, 758, 1397
MaeII ACGT 8 cut(s) 29, 55, 504, 834, 1320, 1346, 1538, 1563
MaeIII GTNAC 5 cut(s) 108, 424, 1297, 1457, 1505
MalI GATC 7 cut(s) 49, 412, 533, 929, 1122, 1439, 1464
MbiI CCGCTC 2 cut(s) 361, 1213
MboI GATC 7 cut(s) 47, 410, 531, 927, 1120, 1437, 1462
MflI RGATCY 3 cut(s) 410, 1120, 1437
MhlI GDGCHC 5 cut(s) 396, 403, 450, 1197, 1346
MluCI AATT 2 cut(s) 130, 1163
MlyI GAGTC 6 cut(s) 371, 653, 830, 885, 1010, 1539
MmeI TCCRAC 5 cut(s) 259, 405, 438, 969, 1058
MroI TCCGGA 1 cut(s) 1283
MroNI GCCGGC 2 cut(s) 710, 887
MseI TTAA 1 cut(s) 959
MslI CAYNNNNRTG 2 cut(s) 977, 983
MspR9I CCNGG 8 cut(s) 398, 778, 873, 1070, 1339, 1372, 1446, 1475
Mva1269I GAATGC 1 cut(s) 1051
MvaI CCWGG 3 cut(s) 873, 1070, 1475
MvnI CGCG 4 cut(s) 20, 1034, 1454, 1527
NaeI GCCGGC 2 cut(s) 712, 889
NciI CCSGG 5 cut(s) 398, 778, 1339, 1372, 1446
NdeII GATC 7 cut(s) 47, 410, 531, 927, 1120, 1437, 1462
NgoMIV GCCGGC 2 cut(s) 710, 887
NlaIII CATG 6 cut(s) 211, 221, 757, 793, 952, 982
NmuCI GTSAC 3 cut(s) 108, 424, 1297
PaeR7I CTCGAG 1 cut(s) 641
PaqCI CACCTGC 1 cut(s) 1285
PciSI GCTCTTC 2 cut(s) 1202, 1322
PcsI WCGNNNNNNNCGW 5 cut(s) 12, 52, 738, 927, 1001
PctI GAATGC 1 cut(s) 1051
PdiI GCCGGC 2 cut(s) 712, 889
PfeI GAWTC 1 cut(s) 297
PflMI CCANNNNNTGG 2 cut(s) 208, 355
PkrI GCNGC 8 cut(s) 271, 463, 946, 1051, 1054, 1386, 1558, 1602
PleI GAGTC 6 cut(s) 371, 652, 829, 884, 1010, 1538
PmaCI CACGTG 3 cut(s) 30, 1347, 1539
PmlI CACGTG 3 cut(s) 30, 1347, 1539
PpsI GAGTC 6 cut(s) 371, 652, 829, 884, 1010, 1538
Ppu21I YACGTR 3 cut(s) 30, 1347, 1539
PpuMI RGGWCCY 3 cut(s) 34, 780, 1098
Psp124BI GAGCTC 1 cut(s) 450
Psp5II RGGWCCY 3 cut(s) 34, 780, 1098
Psp6I CCWGG 3 cut(s) 871, 1068, 1473
PspCI CACGTG 3 cut(s) 30, 1347, 1539
PspEI GGTNACC 1 cut(s) 424
PspFI CCCAGC 1 cut(s) 385
PspGI CCWGG 3 cut(s) 871, 1068, 1473
PspOMI GGGCCC 1 cut(s) 399
PspPPI RGGWCCY 3 cut(s) 34, 780, 1098
PspXI VCTCGAGB 1 cut(s) 641
PstI CTGCAG 1 cut(s) 1389
PsuI RGATCY 3 cut(s) 410, 1120, 1437
RsaI GTAC 5 cut(s) 126, 323, 965, 1223, 1613
RsaNI GTAC 5 cut(s) 125, 322, 964, 1222, 1612
RseI CAYNNNNRTG 2 cut(s) 977, 983
SacI GAGCTC 1 cut(s) 450
SalI GTCGAC 2 cut(s) 5, 431
SapI GCTCTTC 2 cut(s) 1202, 1322
SaqAI TTAA 1 cut(s) 959
SatI GCNGC 8 cut(s) 270, 462, 945, 1050, 1053, 1385, 1557, 1601
Sau3AI GATC 7 cut(s) 47, 410, 531, 927, 1120, 1437, 1462
SchI GAGTC 6 cut(s) 371, 653, 830, 885, 1010, 1539
ScrFI CCNGG 8 cut(s) 398, 778, 873, 1070, 1339, 1372, 1446, 1475
SduI GDGCHC 5 cut(s) 396, 403, 450, 1197, 1346
SexAI ACCWGGT 1 cut(s) 1068
SfaNI GCATC 2 cut(s) 171, 256
SfcI CTRYAG 1 cut(s) 1385
Sfr274I CTCGAG 1 cut(s) 641
SinI GGWCC 7 cut(s) 34, 493, 780, 1072, 1098, 1169, 1449
SlaI CTCGAG 1 cut(s) 641
SmiMI CAYNNNNRTG 2 cut(s) 977, 983
SmlI CTYRAG 2 cut(s) 641, 1105
SmoI CTYRAG 2 cut(s) 641, 1105
Sse9I AATT 2 cut(s) 130, 1163
SspMI CTAG 4 cut(s) 260, 404, 758, 1397
SstI GAGCTC 1 cut(s) 450
StyD4I CCNGG 8 cut(s) 396, 776, 871, 1068, 1337, 1370, 1444, 1473
StyI CCWWGG 2 cut(s) 349, 843
TaaI ACNGT 6 cut(s) 108, 430, 1129, 1233, 1240, 1505
TaiI ACGT 8 cut(s) 32, 58, 507, 837, 1323, 1349, 1541, 1566
TaqII GACCGA 2 cut(s) 265, 1157
TasI AATT 2 cut(s) 130, 1163
TauI GCSGC 4 cut(s) 464, 947, 1055, 1559
TfiI GAWTC 1 cut(s) 297
Tru1I TTAA 1 cut(s) 959
Tru9I TTAA 1 cut(s) 959
TscAI CASTG 1 cut(s) 111
TseFI GTSAC 3 cut(s) 108, 424, 1297
TseI GCWGC 4 cut(s) 269, 1049, 1384, 1600
Tsp45I GTSAC 3 cut(s) 108, 424, 1297
TspDTI ATGAA 2 cut(s) 243, 620
TspGWI ACGGA 4 cut(s) 710, 851, 890, 1064
TspRI CASTG 1 cut(s) 111
Van91I CCANNNNNTGG 2 cut(s) 208, 355
VpaK11BI GGWCC 7 cut(s) 34, 493, 780, 1072, 1098, 1169, 1449
XapI RAATTY 1 cut(s) 1163
XhoI CTCGAG 1 cut(s) 641
XmiI GTMKAC 2 cut(s) 6, 432
XspI CTAG 4 cut(s) 260, 404, 758, 1397
ZraI GACGTC 1 cut(s) 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.