Rh2BG546200

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0015945
Physical Location & Seq
Reverse (-)
75487341 .. 75490160
2820 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG546200.1

Sequence Viewer

Length: 1464 bp
ATGGCCATGGCTCGCCGCCACGTCACTCTCCTCTGCTGCTCGCTTCTGCTCCTCCTCTCCGTCTTGGCTCCACATTCAGATGCGGGTTCAGTCGGAGTGAACTACGGCAGAATCGCCGACAACCTTCCGTCGGCCTACAAGGTTGCTGCCCTTATCAAGTCTCAGGGCTTGGAGCGGGTCAAGGTCTTTGATTCCGACCCGGCCGTGCTCCGGGCCTTATCCGGATCCGGAATCAAGGTCACTGTAGATCTGCCCAACGAGCTTCTCTCTTCGGCGGCCAAGGCCCAGATGTTCGCCACCAACTGGGTCCAGCGCAACGTCGTCGCTTACCATCCCAACACCCAAATCGAAGCCATTGCCGTCGGGAACGAGGTCTTCGTCGACACCCACAACACCACCAAGTACCTCATCGCCGCCATGAAGAACATCCACACCGCCCTCGTCAAGTTCAACCTCGCCTCCGACATCAAGGTCTCCTCCCCCATTGCCCTCAGCGCCCTCCAGAACTCGTACCCGTCCTCCGCCGGGTCATTCCGACCGGAGCTCGTCGAGCCCGTCTTCAAGCCCATGCTCGACTTCCTCCGTCAAACAGGGTCCTACCTCATGGTGAACGCTTACCCGTACTTCGCCTACGAGTCTAACGCTGACGTCATCTCCTTGGACTACGCTCTGTTCCGCCAGAACCCCGGTGTGGTCGACGCCGGCAGCAACCTCCGTTACTTCAGCCTCTTCGACGCCCAAATCGACGCCGTTTTCGCCGCCATGAGCGCCCTTAAATACGACGACATTGAGATGGTCGTTTCCGAAACCGGTTGGCCTTCCAAGGGAGACGAGAATGAGGTCGGAGCCAGCCTCGAAAACGCAGCTGCCTACAACGGAAACCTCGTCCGTCGGATCCTAACCGGCGGTGGGACCCCACTGCGCCCGAAAGCAGATCTGACCGTCTATCTCTTCGCTCTCTTCAACGAGGACAGGAAGAACGGCCCCACATCGGAGAGAAACTACGGCCTGTTCTTCCCCAACGAGAAGAAAGTGTACGACATCCCGTTCACCGTGGAAGGCCTTAAAAACTACCACGACCACCAGTCTCCTGTTTCCGGCAACCAACACGAGACATCTCCGGTGACTTCGCCTGATACCGGGACAGGTGACGTCTCCAAGACTATGACCGGGAACACGTGGTGCGTGGCGAATGGTGAGGCGGGTAAAGAGAAGCTGCAGGCGGCTCTAGATTTCGCTTGCGGTGAGGGAGGTGCTGATTGCCATCAGATCCAGCCCGGTTCCACGTGTTACGATCCTAACACTCTCGAGGCCCACGCCTCGTTCGCGTTCAATAGCTATTACCAGAAAAAGGCGCGCGGAGCCGGCACGTGCTATTTTGGCGGGGCGGCCTACGTAGTCTCGCAGCCACCTAGTAAGTACTCTATCATTACTCCTCTAGTAAATAATTTCGGCGCATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_17 X8

Protein Analysis

487

Amino Acids

52.62

Weight (kDa)

5.83

Isoelectric Point (pI)

30.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 31 - 350 2.4e-88 Glycosyl hydrolases family 17
X8 PF07983 393 - 464 6.8e-23 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015945)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G05790
fragaria_vesca FvH4_6g42190
malus_domestica MD09G1113900.v1.1 MD17G1104000.v1.1
prunus_persica Prupe.3G210700_v2.0.a1
pyrus_communis pycom09g03790
rosa_chinensis RchiOBHm_Chr2g0158111
rosa_laevigata RLG00000021060
rosa_multiflora Rmu_sc0005982.1_g000010
rosa_roxburghii Rroxscaffold_2G00091660
rosa_samantha Rh2AG532100 Rh2BG546200 Rh2CG516000 Rh2DG554700
rosa_wichuraiana Rw2G043990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 470
AatII GACGTC 2 cut(s) 651, 1155
AccB7I CCANNNNNTGG 1 cut(s) 303
AccBSI CCGCTC 1 cut(s) 175
AccI GTMKAC 2 cut(s) 381, 696
AccII CGCG 3 cut(s) 1328, 1357, 1359
AccIII TCCGGA 2 cut(s) 221, 227
AclWI GGATC 6 cut(s) 219, 232, 889, 902, 1264, 1289
AcoI YGGCCR 3 cut(s) 3, 201, 276
AcuI CTGAAG 1 cut(s) 706
AcvI CACGTG 3 cut(s) 1179, 1287, 1371
AcyI GRCGYC 5 cut(s) 648, 699, 735, 747, 1152
AdeI CACNNNGTG 1 cut(s) 1182
AfaI GTAC 5 cut(s) 404, 512, 623, 1037, 1421
AflIII ACRYGT 2 cut(s) 1176, 1286
AgeI ACCGGT 1 cut(s) 809
AgsI TTSAA 4 cut(s) 451, 562, 964, 1333
AhdI GACNNNNNGTC 1 cut(s) 1084
AjiI CACGTC 1 cut(s) 22
AluBI AGCT 5 cut(s) 262, 544, 866, 1216, 1338
AluI AGCT 5 cut(s) 262, 544, 866, 1216, 1338
Alw21I GWGCWC 2 cut(s) 210, 546
Alw26I GTCTC 7 cut(s) 165, 478, 822, 1092, 1106, 1159, 1405
AlwI GGATC 6 cut(s) 219, 232, 889, 902, 1264, 1289
Ama87I CYCGRG 1 cut(s) 1307
Aor13HI TCCGGA 2 cut(s) 221, 227
ApeKI GCWGC 7 cut(s) 36, 146, 705, 863, 866, 1216, 1405
ArsI GACNNNNNNTTYG 2 cut(s) 737, 769
AsiGI ACCGGT 1 cut(s) 809
AspLEI GCGC 7 cut(s) 315, 497, 770, 924, 1357, 1359, 1457
AspS9I GGNCC 7 cut(s) 213, 283, 307, 594, 912, 983, 1312
AsuC2I CCSGG 7 cut(s) 200, 212, 526, 687, 1141, 1171, 1278
AsuHPI GGTGA 6 cut(s) 619, 1042, 1135, 1160, 1208, 1256
AvaI CYCGRG 1 cut(s) 1307
AvaII GGWCC 3 cut(s) 307, 594, 912
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 2 cut(s) 224, 894
BanII GRGCYC 2 cut(s) 546, 555
BarI GAAGNNNNNNTAC 2 cut(s) 1019, 1051
BauI CACGAG 1 cut(s) 1109
BbrPI CACGTG 3 cut(s) 1179, 1287, 1371
BbsI GAAGAC 2 cut(s) 367, 550
Bbv12I GWGCWC 2 cut(s) 210, 546
BbvCI CCTCAGC 1 cut(s) 491
BbvI GCAGC 7 cut(s) 23, 133, 717, 853, 875, 1203, 1417
BccI CCATC 3 cut(s) 339, 787, 1272
BceAI ACGGC 6 cut(s) 121, 188, 344, 734, 997, 1021
BcgI CGANNNNNNTGC 4 cut(s) 304, 338, 338, 372
BcnI CCSGG 7 cut(s) 200, 212, 526, 687, 1141, 1171, 1278
BcoDI GTCTC 7 cut(s) 165, 478, 822, 1092, 1106, 1159, 1405
BfaI CTAG 3 cut(s) 1229, 1413, 1439
BfmI CTRYAG 2 cut(s) 243, 1217
BfoI RGCGCY 2 cut(s) 498, 771
BglII AGATCT 2 cut(s) 247, 934
BmcAI AGTACT 1 cut(s) 1421
Bme1390I CCNGG 7 cut(s) 200, 212, 526, 687, 1141, 1171, 1278
Bme18I GGWCC 3 cut(s) 307, 594, 912
BmeRI GACNNNNNGTC 1 cut(s) 1084
BmeT110I CYCGRG 1 cut(s) 1307
BmgBI CACGTC 1 cut(s) 22
BmgT120I GGNCC 7 cut(s) 213, 283, 307, 594, 912, 983, 1312
BmrFI CCNGG 7 cut(s) 200, 212, 526, 687, 1141, 1171, 1278
BmrI ACTGGG 1 cut(s) 313
BmsI GCATC 1 cut(s) 70
BmuI ACTGGG 1 cut(s) 313
BpiI GAAGAC 2 cut(s) 367, 550
BplI GAGNNNNNCTC 4 cut(s) 251, 283, 837, 869
BpmI CTGGAG 1 cut(s) 485
Bpu10I CCTNAGC 1 cut(s) 491
BpuMI CCSGG 7 cut(s) 200, 212, 526, 687, 1141, 1171, 1278
BsaAI YACGTR 4 cut(s) 1179, 1287, 1371, 1396
BsaBI GATNNNNATC 1 cut(s) 1263
BsaHI GRCGYC 5 cut(s) 648, 699, 735, 747, 1152
BsaI GGTCTC 1 cut(s) 478
BsaJI CCNNGG 6 cut(s) 6, 279, 657, 685, 822, 1053
BsaWI WCCGGW 5 cut(s) 221, 227, 538, 809, 1120
BsaXI ACNNNNNCTCC 6 cut(s) 443, 473, 503, 533, 638, 668
Bse118I RCCGGY 4 cut(s) 701, 809, 902, 1364
Bse1I ACTGG 2 cut(s) 308, 1084
Bse3DI GCAATG 2 cut(s) 354, 483
Bse8I GATNNNNATC 1 cut(s) 1263
BseAI TCCGGA 2 cut(s) 221, 227
BseDI CCNNGG 6 cut(s) 6, 279, 657, 685, 822, 1053
BseGI GGATG 3 cut(s) 331, 426, 1041
BseJI GATNNNNATC 1 cut(s) 1263
BseMI GCAATG 2 cut(s) 354, 483
BseMII CTCAG 2 cut(s) 176, 505
BseNI ACTGG 2 cut(s) 308, 1084
BsePI GCGCGC 1 cut(s) 1355
BseRI GAGGAG 5 cut(s) 20, 41, 44, 466, 1425
BseX3I CGGCCG 1 cut(s) 201
BseXI GCAGC 7 cut(s) 23, 133, 717, 853, 875, 1203, 1417
Bsh1236I CGCG 3 cut(s) 1328, 1357, 1359
Bsh1285I CGRYCG 2 cut(s) 204, 539
BshTI ACCGGT 1 cut(s) 809
BsiEI CGRYCG 2 cut(s) 204, 539
BsiHKAI GWGCWC 2 cut(s) 210, 546
BsiHKCI CYCGRG 1 cut(s) 1307
BslFI GGGAC 2 cut(s) 925, 1156
BsmAI GTCTC 7 cut(s) 165, 478, 822, 1092, 1106, 1159, 1405
BsmBI CGTCTC 2 cut(s) 822, 1159
BsmFI GGGAC 2 cut(s) 925, 1156
Bso31I GGTCTC 1 cut(s) 478
BsoBI CYCGRG 1 cut(s) 1307
Bsp1286I GDGCHC 3 cut(s) 210, 546, 555
Bsp13I TCCGGA 2 cut(s) 221, 227
Bsp143I GATC 6 cut(s) 224, 247, 894, 934, 1269, 1294
Bsp19I CCATGG 1 cut(s) 6
BspCNI CTCAG 2 cut(s) 175, 504
BspEI TCCGGA 2 cut(s) 221, 227
BspFNI CGCG 3 cut(s) 1328, 1357, 1359
BspMAI CTGCAG 1 cut(s) 1221
BspPI GGATC 6 cut(s) 219, 232, 889, 902, 1264, 1289
BspTNI GGTCTC 1 cut(s) 478
BsrBI CCGCTC 1 cut(s) 175
BsrDI GCAATG 2 cut(s) 354, 483
BsrFI RCCGGY 4 cut(s) 701, 809, 902, 1364
BsrI ACTGG 2 cut(s) 308, 1084
BssAI RCCGGY 4 cut(s) 701, 809, 902, 1364
BssECI CCNNGG 6 cut(s) 6, 279, 657, 685, 822, 1053
BssHII GCGCGC 1 cut(s) 1355
BssMI GATC 6 cut(s) 224, 247, 894, 934, 1269, 1294
BssNI GRCGYC 5 cut(s) 648, 699, 735, 747, 1152
BssSI CACGAG 1 cut(s) 1109
BssT1I CCWWGG 4 cut(s) 6, 279, 657, 822
Bst2BI CACGAG 1 cut(s) 1109
Bst4CI ACNGT 3 cut(s) 244, 943, 1054
Bst6I CTCTTC 4 cut(s) 274, 734, 956, 965
BstACI GRCGYC 5 cut(s) 648, 699, 735, 747, 1152
BstBAI YACGTR 4 cut(s) 1179, 1287, 1371, 1396
BstC8I GCNNGC 8 cut(s) 13, 41, 703, 850, 1221, 1240, 1357, 1366
BstDEI CTNAG 2 cut(s) 162, 491
BstDSI CCRYGG 2 cut(s) 6, 1053
BstF5I GGATG 3 cut(s) 331, 426, 1041
BstFNI CGCG 3 cut(s) 1328, 1357, 1359
BstH2I RGCGCY 2 cut(s) 498, 771
BstHHI GCGC 7 cut(s) 315, 497, 770, 924, 1357, 1359, 1457
BstKTI GATC 6 cut(s) 227, 250, 897, 937, 1272, 1297
BstMAI GTCTC 7 cut(s) 165, 478, 822, 1092, 1106, 1159, 1405
BstMBI GATC 6 cut(s) 224, 247, 894, 934, 1269, 1294
BstMCI CGRYCG 2 cut(s) 204, 539
BstNSI RCATGY 1 cut(s) 1461
BstSCI CCNGG 7 cut(s) 198, 210, 524, 685, 1139, 1169, 1276
BstSFI CTRYAG 2 cut(s) 243, 1217
BstSNI TACGTA 1 cut(s) 1396
BstUI CGCG 3 cut(s) 1328, 1357, 1359
BstV1I GCAGC 7 cut(s) 23, 133, 717, 853, 875, 1203, 1417
BstV2I GAAGAC 2 cut(s) 367, 550
BstX2I RGATCY 5 cut(s) 224, 247, 894, 934, 1269
BstYI RGATCY 5 cut(s) 224, 247, 894, 934, 1269
BstZI CGGCCG 1 cut(s) 201
BtgI CCRYGG 2 cut(s) 6, 1053
BtgZI GCGATG 1 cut(s) 394
BtrI CACGTC 1 cut(s) 22
BtsCI GGATG 3 cut(s) 331, 426, 1041
BtsI GCAGTG 1 cut(s) 917
BtsIMutI CAGTG 2 cut(s) 240, 917
Cac8I GCNNGC 8 cut(s) 13, 41, 703, 850, 1221, 1240, 1357, 1366
CfoI GCGC 7 cut(s) 315, 497, 770, 924, 1357, 1359, 1457
Cfr10I RCCGGY 4 cut(s) 701, 809, 902, 1364
Cfr13I GGNCC 7 cut(s) 213, 283, 307, 594, 912, 983, 1312
CseI GACGC 3 cut(s) 707, 743, 755
Csp6I GTAC 5 cut(s) 403, 511, 622, 1036, 1420
CspAI ACCGGT 1 cut(s) 809
CviAII CATG 6 cut(s) 7, 418, 568, 604, 763, 1458
CviQI GTAC 5 cut(s) 403, 511, 622, 1036, 1420
DdeI CTNAG 2 cut(s) 162, 491
DpnI GATC 6 cut(s) 226, 249, 896, 936, 1271, 1296
DpnII GATC 6 cut(s) 224, 247, 894, 934, 1269, 1294
DraIII CACNNNGTG 1 cut(s) 1182
DrdI GACNNNNNNGTC 1 cut(s) 470
DriI GACNNNNNGTC 1 cut(s) 1084
DseDI GACNNNNNNGTC 1 cut(s) 470
EaeI YGGCCR 3 cut(s) 3, 201, 276
EagI CGGCCG 1 cut(s) 201
Eam1104I CTCTTC 4 cut(s) 274, 734, 956, 965
Eam1105I GACNNNNNGTC 1 cut(s) 1084
EarI CTCTTC 4 cut(s) 274, 734, 956, 965
EciI GGCGGA 2 cut(s) 511, 665
Ecl136II GAGCTC 1 cut(s) 544
EclXI CGGCCG 1 cut(s) 201
Eco105I TACGTA 1 cut(s) 1396
Eco130I CCWWGG 4 cut(s) 6, 279, 657, 822
Eco147I AGGCCT 1 cut(s) 1062
Eco24I GRGCYC 2 cut(s) 546, 555
Eco31I GGTCTC 1 cut(s) 478
Eco47I GGWCC 3 cut(s) 307, 594, 912
Eco52I CGGCCG 1 cut(s) 201
Eco53kI GAGCTC 1 cut(s) 544
Eco57I CTGAAG 1 cut(s) 706
Eco72I CACGTG 3 cut(s) 1179, 1287, 1371
Eco88I CYCGRG 1 cut(s) 1307
EcoICRI GAGCTC 1 cut(s) 544
EcoO109I RGGNCCY 2 cut(s) 594, 912
EcoT14I CCWWGG 4 cut(s) 6, 279, 657, 822
EcoT38I GRGCYC 2 cut(s) 546, 555
ErhI CCWWGG 4 cut(s) 6, 279, 657, 822
Esp3I CGTCTC 2 cut(s) 822, 1159
FaeI CATG 6 cut(s) 10, 421, 571, 607, 766, 1461
FaiI YATR 7 cut(s) 8, 419, 569, 605, 764, 1166, 1459
FaqI GGGAC 2 cut(s) 925, 1156
FatI CATG 6 cut(s) 6, 417, 567, 603, 762, 1457
FauI CCCGC 4 cut(s) 76, 168, 1195, 1376
FblI GTMKAC 2 cut(s) 381, 696
FokI GGATG 3 cut(s) 318, 413, 1028
FriOI GRGCYC 2 cut(s) 546, 555
FspBI CTAG 3 cut(s) 1229, 1413, 1439
GlaI GCGC 7 cut(s) 314, 496, 769, 923, 1356, 1358, 1456
GsuI CTGGAG 1 cut(s) 485
HaeII RGCGCY 2 cut(s) 498, 771
HgaI GACGC 3 cut(s) 707, 743, 755
HhaI GCGC 7 cut(s) 315, 497, 770, 924, 1357, 1359, 1457
Hin1I GRCGYC 5 cut(s) 648, 699, 735, 747, 1152
Hin1II CATG 6 cut(s) 10, 421, 571, 607, 766, 1461
Hin6I GCGC 7 cut(s) 313, 495, 768, 922, 1355, 1357, 1455
HinP1I GCGC 7 cut(s) 313, 495, 768, 922, 1355, 1357, 1455
HincII GTYRAC 2 cut(s) 382, 697
HindII GTYRAC 2 cut(s) 382, 697
HinfI GANTC 4 cut(s) 111, 191, 231, 635
HphI GGTGA 6 cut(s) 619, 1042, 1135, 1160, 1208, 1256
Hpy166II GTNNAC 6 cut(s) 100, 382, 610, 697, 1036, 1050
Hpy188III TCNNGA 6 cut(s) 222, 228, 364, 502, 1229, 1307
Hpy8I GTNNAC 6 cut(s) 100, 382, 610, 697, 1036, 1050
HpyAV CCTTC 3 cut(s) 134, 828, 1052
HpyCH4III ACNGT 3 cut(s) 244, 943, 1054
HpyCH4IV ACGT 8 cut(s) 21, 318, 648, 1152, 1178, 1286, 1370, 1395
HpyCH4V TGCA 1 cut(s) 1219
HpyF3I CTNAG 2 cut(s) 162, 491
HpySE526I ACGT 8 cut(s) 21, 318, 648, 1152, 1178, 1286, 1370, 1395
Hsp92I GRCGYC 5 cut(s) 648, 699, 735, 747, 1152
Hsp92II CATG 6 cut(s) 10, 421, 571, 607, 766, 1461
HspAI GCGC 7 cut(s) 313, 495, 768, 922, 1355, 1357, 1455
KflI GGGWCCC 1 cut(s) 912
Kpn2I TCCGGA 2 cut(s) 221, 227
KroI GCCGGC 2 cut(s) 701, 1364
KroNI GCCGGC 2 cut(s) 703, 1366
Kzo9I GATC 6 cut(s) 224, 247, 894, 934, 1269, 1294
LmnI GCTCC 7 cut(s) 54, 73, 172, 213, 541, 845, 1361
Lsp1109I GCAGC 7 cut(s) 23, 133, 717, 853, 875, 1203, 1417
LweI GCATC 1 cut(s) 70
MaeI CTAG 3 cut(s) 1229, 1413, 1439
MaeII ACGT 8 cut(s) 21, 318, 648, 1152, 1178, 1286, 1370, 1395
MaeIII GTNAC 6 cut(s) 22, 238, 716, 1123, 1148, 1289
MalI GATC 6 cut(s) 226, 249, 896, 936, 1271, 1296
MbiI CCGCTC 1 cut(s) 175
MboI GATC 6 cut(s) 224, 247, 894, 934, 1269, 1294
MflI RGATCY 5 cut(s) 224, 247, 894, 934, 1269
MhlI GDGCHC 3 cut(s) 210, 546, 555
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 1447
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 644
MmeI TCCRAC 6 cut(s) 73, 219, 486, 559, 823, 872
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 2 cut(s) 221, 227
MroNI GCCGGC 2 cut(s) 701, 1364
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 774, 1065
MslI CAYNNNNRTG 2 cut(s) 78, 791
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 866
MspR9I CCNGG 7 cut(s) 200, 212, 526, 687, 1141, 1171, 1278
MvnI CGCG 3 cut(s) 1328, 1357, 1359
NaeI GCCGGC 2 cut(s) 703, 1366
NciI CCSGG 7 cut(s) 200, 212, 526, 687, 1141, 1171, 1278
NcoI CCATGG 1 cut(s) 6
NdeII GATC 6 cut(s) 224, 247, 894, 934, 1269, 1294
NgoMIV GCCGGC 2 cut(s) 701, 1364
NlaIII CATG 6 cut(s) 10, 421, 571, 607, 766, 1461
NmuCI GTSAC 4 cut(s) 22, 238, 1123, 1148
NspI RCATGY 1 cut(s) 1461
PaeR7I CTCGAG 1 cut(s) 1307
PauI GCGCGC 1 cut(s) 1355
PceI AGGCCT 1 cut(s) 1062
PcsI WCGNNNNNNNCGW 4 cut(s) 375, 552, 741, 882
PdiI GCCGGC 2 cut(s) 703, 1366
PfeI GAWTC 3 cut(s) 111, 191, 231
PflMI CCANNNNNTGG 1 cut(s) 303
PinAI ACCGGT 1 cut(s) 809
PleI GAGTC 1 cut(s) 643
PmaCI CACGTG 3 cut(s) 1179, 1287, 1371
PmlI CACGTG 3 cut(s) 1179, 1287, 1371
PpsI GAGTC 1 cut(s) 643
Ppu21I YACGTR 4 cut(s) 1179, 1287, 1371, 1396
PpuMI RGGWCCY 2 cut(s) 594, 912
Psp124BI GAGCTC 1 cut(s) 546
Psp5II RGGWCCY 2 cut(s) 594, 912
PspCI CACGTG 3 cut(s) 1179, 1287, 1371
PspPI GGNCC 7 cut(s) 213, 283, 307, 594, 912, 983, 1312
PspPPI RGGWCCY 2 cut(s) 594, 912
PstI CTGCAG 1 cut(s) 1221
PsuI RGATCY 5 cut(s) 224, 247, 894, 934, 1269
PteI GCGCGC 1 cut(s) 1355
PvuII CAGCTG 1 cut(s) 866
RsaI GTAC 5 cut(s) 404, 512, 623, 1037, 1421
RsaNI GTAC 5 cut(s) 403, 511, 622, 1036, 1420
RseI CAYNNNNRTG 2 cut(s) 78, 791
SacI GAGCTC 1 cut(s) 546
SalI GTCGAC 2 cut(s) 380, 695
SaqAI TTAA 2 cut(s) 774, 1065
Sau3AI GATC 6 cut(s) 224, 247, 894, 934, 1269, 1294
Sau96I GGNCC 7 cut(s) 213, 283, 307, 594, 912, 983, 1312
ScaI AGTACT 1 cut(s) 1421
SchI GAGTC 1 cut(s) 644
ScrFI CCNGG 7 cut(s) 200, 212, 526, 687, 1141, 1171, 1278
SduI GDGCHC 3 cut(s) 210, 546, 555
SfaNI GCATC 1 cut(s) 70
SfcI CTRYAG 2 cut(s) 243, 1217
Sfr274I CTCGAG 1 cut(s) 1307
SinI GGWCC 3 cut(s) 307, 594, 912
SlaI CTCGAG 1 cut(s) 1307
SmiMI CAYNNNNRTG 2 cut(s) 78, 791
SmlI CTYRAG 1 cut(s) 1307
SmoI CTYRAG 1 cut(s) 1307
SnaBI TACGTA 1 cut(s) 1396
Sse9I AATT 1 cut(s) 1447
SseBI AGGCCT 1 cut(s) 1062
SspMI CTAG 3 cut(s) 1229, 1413, 1439
SstI GAGCTC 1 cut(s) 546
StuI AGGCCT 1 cut(s) 1062
StyD4I CCNGG 7 cut(s) 198, 210, 524, 685, 1139, 1169, 1276
StyI CCWWGG 4 cut(s) 6, 279, 657, 822
TaaI ACNGT 3 cut(s) 244, 943, 1054
TaiI ACGT 8 cut(s) 24, 321, 651, 1155, 1181, 1289, 1373, 1398
TaqI TCGA 9 cut(s) 348, 381, 549, 573, 696, 732, 744, 855, 1308
TasI AATT 1 cut(s) 1447
TatI WGTACW 1 cut(s) 1419
TauI GCSGC 6 cut(s) 18, 278, 416, 761, 1226, 1391
TfiI GAWTC 3 cut(s) 111, 191, 231
Tru1I TTAA 2 cut(s) 774, 1065
Tru9I TTAA 2 cut(s) 774, 1065
TscAI CASTG 2 cut(s) 247, 924
TseFI GTSAC 4 cut(s) 22, 238, 1123, 1148
TseI GCWGC 7 cut(s) 36, 146, 705, 863, 866, 1216, 1405
Tsp45I GTSAC 4 cut(s) 22, 238, 1123, 1148
TspDTI ATGAA 1 cut(s) 434
TspGWI ACGGA 6 cut(s) 49, 117, 572, 704, 878, 891
TspRI CASTG 2 cut(s) 247, 924
Van91I CCANNNNNTGG 1 cut(s) 303
VpaK11BI GGWCC 3 cut(s) 307, 594, 912
XbaI TCTAGA 1 cut(s) 1228
XceI RCATGY 1 cut(s) 1461
XhoI CTCGAG 1 cut(s) 1307
XmiI GTMKAC 2 cut(s) 381, 696
XspI CTAG 3 cut(s) 1229, 1413, 1439
ZraI GACGTC 2 cut(s) 649, 1153
ZrmI AGTACT 1 cut(s) 1421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.