Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0005140

Physical Location & Seq

Genomic Coordinates Forward (+)

8985665 .. 8988976

Gene Structure 3312 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1116800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1395 bp

ATGTGGTTCAGAAAATTAAGGAATGTGGATGGTGAGAATTTTGTGGGGTTTTCAAGGCTAATCAGAAGATATGCATCAGTTTCAAGCACAAGTGTGATAGACATGGCTGTTAGAGATGGGGAGCTGAGGGTTTTCATAGTCTCTGGAGAGGTTTCCGGAGACACCATTGGCTCTCGTCTCATGGCGTCTCTGAAGAATCTCTGTCCTGTTCCTGTCCGTTTTGCCGGTGTTGGAGGGTTGATGATGTCCAAGCAAGGATTAAAGTCGCTGTTTCCGATGGAGGATATTGCGGTGATGGGATTATTGGAGTTATTGCCTCATCTGAATAGGATCAGAGTGAAGCTGCTGGAGACACTAGAGGCTGCTCTTCTGTTTCAACCTCATGTTGTTGTGACAGTGGATTCAAAGGGTTTCTCTTTCCGCCTCTTAAAACAGCTACGGGCTAGATACAATCAGCAGCGAGTCGATGCTCCAGTACACTTCCACTATGTAGCACCGTCATTCTGGGCTTGGAAAGGGGGTGAAGCAAGACTCCGAGGCCTGGCTGGATTTGTGGATCATGTCTTGTGTATACTTCCAAATGAAGAAGAAGTTTGCAGATCAAATGGACTGGCTGCGACCTTTGTGGGCCACCCCATTTTGGAGGATGTTTTGGAGCTGAAATCGAAGGACACTTCACCCCATGAATGGAAGATAGAAGGAAACTGTGAAGATTTCCGAAGGAGATATGCAATACCAGCAGGAGCAAAAGTCATTTCCTTGCTTCCTGGAAGCAGATTACAAGAGGTCACCAGAATGCTTTCTATCTTCGCAAACGCTATGGGATTATTAAAAGTGTCCTTTCCTGAGTTAGTAACAGTCATCCACGTTGCTCCAAATCAGCATGTGAAGAACTATGTTGCCGGAATCGCTAACAAGTGGCCTGTGCCCTCTATATTGATTCCAGGAGGATCCCTGCACCAAAAATATGATGCATTCAGTGCAAGTAGTGTTGCATTATGTGCTTCTGGAACAGTTGCCTTGGAGATGCAGCTTGCACGGCTACCATGTGTAGTCGCTTATCGAGCTCATTTCCTAACTGAGTGGATTATTCGTTATAAGGCCAAGATACCTTACATCTCTCTCCCAAATATTCTCATGGATTCACCTATTATACCCGAAGCTCTCTTTCAAGCGTGCACTCCGACAAACCTAGCATCATTGATTTTGGGTTTGATACATGACGATGGCCTCCGAGAAAAGCAGATTGTTGCCGCTGGCAAGGTTATCAGACTTCTTTGCACTCCGGCCAGAAATTCAGATGAGTTGGTAGAGCAGCATCAAGGAGGGAGGTTTCCCCATAACACACCAAGTGTGATAGCAGCAACTGCAATACTGCACCATGCAAAGCCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005975 GO:0006040 GO:0006629 GO:0006643 GO:0006644 GO:0006664 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008194 GO:0008289 GO:0008610 GO:0008654 GO:0008915 GO:0009058 GO:0009245 GO:0009247 GO:0009311 GO:0009312 GO:0009987 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0019637 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046467 GO:0046493 GO:0071704 GO:0090407 GO:1901135 GO:1901137 GO:1901269 GO:1901271 GO:1901576 GO:1903509 GO:2001289

KEGG Pathways

Metabolic & Signaling

ko00540 ko01100 map00540 map01100

Pfam Domains

Protein Families

LpxB

Protein Analysis

465

Amino Acids

51.28

Weight (kDa)

9.27

Isoelectric Point (pI)

39.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LpxB	PF02684	44 - 438	2.8e-74	Lipid-A-disaccharide synthetase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005140)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G04560
fragaria_vesca	FvH4_6g41870 FvH4_6g41870 FvH4_6g41870 FvH4_6g41870 FvH4_6g41870 FvH4_6g41870
malus_domestica	MD09G1116800.v1.1
prunus_persica	Prupe.3G208000_v2.0.a1 Prupe.3G208000_v2.0.a1 Prupe.3G208000_v2.0.a1 Prupe.3G208000_v2.0.a1 Prupe.3G208000_v2.0.a1
pyrus_communis	pycom09g04140 pycom17g10060
rosa_chinensis	RchiOBHm_Chr2g0157701 RchiOBHm_Chr2g0157711
rosa_laevigata	RLG00000021009
rosa_multiflora	Rmu_sc0001275.1_g000003
rosa_roxburghii	Rroxscaffold_2G00092020
rosa_rugosa	Rorug02G0462900
rosa_samantha	Rh2AG527200 Rh2AG527300 Rh2BG542000 Rh2BG542100 Rh2CG511700 Rh2CG511800 Rh2DG550600 Rh2DG550700
rosa_wichuraiana	Rw2G043660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1098
AccI	GTMKAC	1 cut(s)	571
AccIII	TCCGGA	1 cut(s)	155
AciI	CCGC	3 cut(s)	290, 421, 1254
AclWI	GGATC	4 cut(s)	338, 564, 945, 958
AcoI	YGGCCR	1 cut(s)	1287
AcsI	RAATTY	2 cut(s)	37, 1294
AcuI	CTGAAG	1 cut(s)	212
AcyI	GRCGYC	1 cut(s)	185
AdeI	CACNNNGTG	1 cut(s)	1352
AfaI	GTAC	1 cut(s)	477
AfiI	CCNNNNNNNGG	3 cut(s)	541, 640, 687
AgsI	TTSAA	5 cut(s)	54, 84, 377, 405, 1172
AjnI	CCWGG	3 cut(s)	540, 766, 943
AleI	CACNNNNGTG	1 cut(s)	92
AluBI	AGCT	7 cut(s)	124, 343, 436, 658, 1033, 1067, 1163
AluI	AGCT	7 cut(s)	124, 343, 436, 658, 1033, 1067, 1163
Alw21I	GWGCWC	2 cut(s)	1069, 1181
Alw26I	GTCTC	5 cut(s)	145, 153, 182, 192, 344
Alw44I	GTGCAC	1 cut(s)	1177
AlwI	GGATC	4 cut(s)	338, 564, 945, 958
AlwNI	CAGNNNCTG	1 cut(s)	1367
Aor13HI	TCCGGA	1 cut(s)	155
AoxI	GGCC	6 cut(s)	538, 628, 920, 1101, 1228, 1287
ApaLI	GTGCAC	1 cut(s)	1177
ApeKI	GCWGC	7 cut(s)	343, 362, 457, 614, 1030, 1315, 1361
ApoI	RAATTY	2 cut(s)	37, 1294
Asp700I	GAANNNNTTC	1 cut(s)	799
AspS9I	GGNCC	1 cut(s)	628
AsuHPI	GGTGA	6 cut(s)	44, 304, 533, 669, 781, 1137
BaeGI	GKGCMC	2 cut(s)	930, 1181
BamHI	GGATCC	1 cut(s)	950
BanII	GRGCYC	1 cut(s)	1069
Bbv12I	GWGCWC	2 cut(s)	1069, 1181
BbvCI	CCTCAGC	1 cut(s)	125
BbvI	GCAGC	7 cut(s)	330, 349, 469, 601, 1042, 1327, 1373
BccI	CCATC	5 cut(s)	23, 110, 271, 289, 1220
BceAI	ACGGC	1 cut(s)	1055
BciT130I	CCWGG	3 cut(s)	542, 768, 945
BcoDI	GTCTC	5 cut(s)	145, 153, 182, 192, 344
BfaI	CTAG	3 cut(s)	356, 444, 1193
BisI	GCNGC	8 cut(s)	344, 363, 458, 615, 1031, 1254, 1316, 1362
BlsI	GCNGC	8 cut(s)	345, 364, 459, 616, 1032, 1255, 1317, 1363
Bme1390I	CCNGG	3 cut(s)	542, 768, 945
BmgT120I	GGNCC	1 cut(s)	628
BmiI	GGNNCC	1 cut(s)	952
BmrFI	CCNGG	3 cut(s)	542, 768, 945
BmsI	GCATC	6 cut(s)	83, 457, 961, 1017, 1205, 1327
BpmI	CTGGAG	3 cut(s)	165, 368, 456
Bpu10I	CCTNAGC	1 cut(s)	125
BsaBI	GATNNNNATC	1 cut(s)	73
BsaHI	GRCGYC	1 cut(s)	185
BsaJI	CCNNGG	2 cut(s)	535, 1020
BsaWI	WCCGGW	1 cut(s)	155
BsaXI	ACNNNNNCTCC	2 cut(s)	735, 765
Bsc4I	CCNNNNNNNGG	3 cut(s)	541, 640, 687
Bse118I	RCCGGY	1 cut(s)	224
Bse1I	ACTGG	2 cut(s)	473, 615
Bse8I	GATNNNNATC	1 cut(s)	73
BseAI	TCCGGA	1 cut(s)	155
BseBI	CCWGG	3 cut(s)	542, 768, 945
BseDI	CCNNGG	2 cut(s)	535, 1020
BseGI	GGATG	3 cut(s)	34, 652, 861
BseJI	GATNNNNATC	1 cut(s)	73
BseLI	CCNNNNNNNGG	3 cut(s)	541, 640, 687
BseMII	CTCAG	3 cut(s)	116, 837, 1071
BseNI	ACTGG	2 cut(s)	473, 615
BseSI	GKGCMC	2 cut(s)	930, 1181
BseXI	GCAGC	7 cut(s)	330, 349, 469, 601, 1042, 1327, 1373
BsgI	GTGCAG	2 cut(s)	941, 1361
BshFI	GGCC	6 cut(s)	540, 630, 922, 1103, 1230, 1289
BsiHKAI	GWGCWC	2 cut(s)	1069, 1181
BsiSI	CCGG	4 cut(s)	156, 225, 903, 1286
BslI	CCNNNNNNNGG	3 cut(s)	541, 640, 687
BsmAI	GTCTC	5 cut(s)	145, 153, 182, 192, 344
BsmBI	CGTCTC	2 cut(s)	182, 192
BsmI	GAATGC	2 cut(s)	801, 974
BsnI	GGCC	6 cut(s)	540, 630, 922, 1103, 1230, 1289
Bsp1286I	GDGCHC	3 cut(s)	930, 1069, 1181
Bsp13I	TCCGGA	1 cut(s)	155
Bsp143I	GATC	4 cut(s)	330, 556, 599, 950
BspACI	CCGC	3 cut(s)	290, 421, 1254
BspANI	GGCC	6 cut(s)	540, 630, 922, 1103, 1230, 1289
BspCNI	CTCAG	3 cut(s)	117, 838, 1072
BspEI	TCCGGA	1 cut(s)	155
BspLI	GGNNCC	1 cut(s)	952
BspPI	GGATC	4 cut(s)	338, 564, 945, 958
BspQI	GCTCTTC	1 cut(s)	372
BsrFI	RCCGGY	1 cut(s)	224
BsrI	ACTGG	2 cut(s)	473, 615
BssAI	RCCGGY	1 cut(s)	224
BssECI	CCNNGG	2 cut(s)	535, 1020
BssMI	GATC	4 cut(s)	330, 556, 599, 950
BssNAI	GTATAC	1 cut(s)	572
BssNI	GRCGYC	1 cut(s)	185
BssT1I	CCWWGG	1 cut(s)	1020
Bst1107I	GTATAC	1 cut(s)	572
Bst2UI	CCWGG	3 cut(s)	542, 768, 945
Bst4CI	ACNGT	5 cut(s)	397, 498, 707, 859, 1015
Bst6I	CTCTTC	1 cut(s)	372
BstACI	GRCGYC	1 cut(s)	185
BstAPI	GCANNNNNTGC	3 cut(s)	980, 1001, 1367
BstC8I	GCNNGC	3 cut(s)	1035, 1177, 1258
BstDEI	CTNAG	3 cut(s)	125, 846, 1080
BstEII	GGTNACC	1 cut(s)	787
BstF5I	GGATG	3 cut(s)	34, 652, 861
BstKTI	GATC	4 cut(s)	333, 559, 602, 953
BstMAI	GTCTC	5 cut(s)	145, 153, 182, 192, 344
BstMBI	GATC	4 cut(s)	330, 556, 599, 950
BstMWI	GCNNNNNNNGC	7 cut(s)	737, 908, 980, 1001, 1039, 1064, 1367
BstNI	CCWGG	3 cut(s)	542, 768, 945
BstNSI	RCATGY	1 cut(s)	887
BstPI	GGTNACC	1 cut(s)	787
BstSCI	CCNGG	3 cut(s)	540, 766, 943
BstSLI	GKGCMC	2 cut(s)	930, 1181
BstV1I	GCAGC	7 cut(s)	330, 349, 469, 601, 1042, 1327, 1373
BstX2I	RGATCY	1 cut(s)	950
BstYI	RGATCY	1 cut(s)	950
BstZ17I	GTATAC	1 cut(s)	572
BsuRI	GGCC	6 cut(s)	540, 630, 922, 1103, 1230, 1289
BtsCI	GGATG	3 cut(s)	34, 652, 861
BtsIMutI	CAGTG	2 cut(s)	402, 985
Cac8I	GCNNGC	3 cut(s)	1035, 1177, 1258
CaiI	CAGNNNCTG	1 cut(s)	1367
Cfr10I	RCCGGY	1 cut(s)	224
Cfr13I	GGNCC	1 cut(s)	628
CseI	GACGC	1 cut(s)	174
Csp6I	GTAC	1 cut(s)	476
CviQI	GTAC	1 cut(s)	476
DdeI	CTNAG	3 cut(s)	125, 846, 1080
DpnI	GATC	4 cut(s)	332, 558, 601, 952
DpnII	GATC	4 cut(s)	330, 556, 599, 950
DraIII	CACNNNGTG	1 cut(s)	1352
EaeI	YGGCCR	1 cut(s)	1287
Eam1104I	CTCTTC	1 cut(s)	372
EarI	CTCTTC	1 cut(s)	372
EciI	GGCGGA	1 cut(s)	410
Ecl136II	GAGCTC	1 cut(s)	1067
Eco130I	CCWWGG	1 cut(s)	1020
Eco147I	AGGCCT	1 cut(s)	540
Eco24I	GRGCYC	1 cut(s)	1069
Eco53kI	GAGCTC	1 cut(s)	1067
Eco57I	CTGAAG	1 cut(s)	212
Eco91I	GGTNACC	1 cut(s)	787
EcoICRI	GAGCTC	1 cut(s)	1067
EcoO65I	GGTNACC	1 cut(s)	787
EcoRII	CCWGG	3 cut(s)	540, 766, 943
EcoT14I	CCWWGG	1 cut(s)	1020
EcoT22I	ATGCAT	2 cut(s)	76, 976
EcoT38I	GRGCYC	1 cut(s)	1069
ErhI	CCWWGG	1 cut(s)	1020
Esp3I	CGTCTC	2 cut(s)	182, 192
FblI	GTMKAC	1 cut(s)	571
Fnu4HI	GCNGC	8 cut(s)	344, 363, 458, 615, 1031, 1254, 1316, 1362
FokI	GGATG	3 cut(s)	41, 659, 848
FriOI	GRGCYC	1 cut(s)	1069
Fsp4HI	GCNGC	8 cut(s)	344, 363, 458, 615, 1031, 1254, 1316, 1362
FspBI	CTAG	3 cut(s)	356, 444, 1193
GluI	GCNGC	8 cut(s)	344, 363, 458, 615, 1031, 1254, 1316, 1362
GsuI	CTGGAG	3 cut(s)	165, 368, 456
HaeIII	GGCC	6 cut(s)	540, 630, 922, 1103, 1230, 1289
HapII	CCGG	4 cut(s)	156, 225, 903, 1286
HgaI	GACGC	1 cut(s)	174
Hin1I	GRCGYC	1 cut(s)	185
HinfI	GANTC	7 cut(s)	196, 401, 462, 531, 906, 940, 1142
HpaII	CCGG	4 cut(s)	156, 225, 903, 1286
HphI	GGTGA	6 cut(s)	44, 304, 533, 669, 781, 1137
Hpy166II	GTNNAC	3 cut(s)	478, 572, 1179
Hpy188III	TCNNGA	4 cut(s)	144, 156, 845, 1008
Hpy8I	GTNNAC	3 cut(s)	478, 572, 1179
HpyAV	CCTTC	3 cut(s)	661, 692, 714
HpyCH4III	ACNGT	5 cut(s)	397, 498, 707, 859, 1015
HpyCH4IV	ACGT	1 cut(s)	867
HpyF10VI	GCNNNNNNNGC	7 cut(s)	737, 908, 980, 1001, 1039, 1064, 1367
HpyF3I	CTNAG	3 cut(s)	125, 846, 1080
HpySE526I	ACGT	1 cut(s)	867
Hsp92I	GRCGYC	1 cut(s)	185
Kpn2I	TCCGGA	1 cut(s)	155
Kzo9I	GATC	4 cut(s)	330, 556, 599, 950
LguI	GCTCTTC	1 cut(s)	372
LmnI	GCTCC	5 cut(s)	121, 475, 655, 743, 877
Lsp1109I	GCAGC	7 cut(s)	330, 349, 469, 601, 1042, 1327, 1373
LweI	GCATC	6 cut(s)	83, 457, 961, 1017, 1205, 1327
MaeI	CTAG	3 cut(s)	356, 444, 1193
MaeII	ACGT	1 cut(s)	867
MaeIII	GTNAC	3 cut(s)	391, 787, 853
MalI	GATC	4 cut(s)	332, 558, 601, 952
MboI	GATC	4 cut(s)	330, 556, 599, 950
MboII	GAAGA	9 cut(s)	78, 205, 359, 596, 599, 703, 722, 799, 901
MflI	RGATCY	1 cut(s)	950
MhlI	GDGCHC	3 cut(s)	930, 1069, 1181
MluCI	AATT	3 cut(s)	14, 37, 1294
MlyI	GAGTC	2 cut(s)	471, 525
MmeI	TCCRAC	2 cut(s)	211, 1208
Mph1103I	ATGCAT	2 cut(s)	76, 976
MroI	TCCGGA	1 cut(s)	155
MroXI	GAANNNNTTC	1 cut(s)	799
MseI	TTAA	4 cut(s)	17, 260, 428, 830
MslI	CAYNNNNRTG	3 cut(s)	92, 794, 1224
MspA1I	CMGCKG	1 cut(s)	1256
MspI	CCGG	4 cut(s)	156, 225, 903, 1286
MspR9I	CCNGG	3 cut(s)	542, 768, 945
Mva1269I	GAATGC	2 cut(s)	801, 974
MvaI	CCWGG	3 cut(s)	542, 768, 945
MwoI	GCNNNNNNNGC	7 cut(s)	737, 908, 980, 1001, 1039, 1064, 1367
NdeII	GATC	4 cut(s)	330, 556, 599, 950
NlaIV	GGNNCC	1 cut(s)	952
NmuCI	GTSAC	2 cut(s)	391, 787
NsiI	ATGCAT	2 cut(s)	76, 976
NspI	RCATGY	1 cut(s)	887
OliI	CACNNNNGTG	1 cut(s)	92
PceI	AGGCCT	1 cut(s)	540
PciSI	GCTCTTC	1 cut(s)	372
PcsI	WCGNNNNNNNCGW	1 cut(s)	272
PctI	GAATGC	2 cut(s)	801, 974
PdmI	GAANNNNTTC	1 cut(s)	799
PfeI	GAWTC	5 cut(s)	196, 401, 906, 940, 1142
PfoI	TCCNGGA	2 cut(s)	766, 943
PkrI	GCNGC	8 cut(s)	345, 364, 459, 616, 1032, 1255, 1317, 1363
PleI	GAGTC	2 cut(s)	470, 525
PpsI	GAGTC	2 cut(s)	470, 525
PsiI	TTATAA	1 cut(s)	1098
Psp124BI	GAGCTC	1 cut(s)	1069
Psp6I	CCWGG	3 cut(s)	540, 766, 943
PspEI	GGTNACC	1 cut(s)	787
PspGI	CCWGG	3 cut(s)	540, 766, 943
PspN4I	GGNNCC	1 cut(s)	952
PspPI	GGNCC	1 cut(s)	628
PstNI	CAGNNNCTG	1 cut(s)	1367
PsuI	RGATCY	1 cut(s)	950
RsaI	GTAC	1 cut(s)	477
RsaNI	GTAC	1 cut(s)	476
RseI	CAYNNNNRTG	3 cut(s)	92, 794, 1224
SacI	GAGCTC	1 cut(s)	1069
SapI	GCTCTTC	1 cut(s)	372
SaqAI	TTAA	4 cut(s)	17, 260, 428, 830
SatI	GCNGC	8 cut(s)	344, 363, 458, 615, 1031, 1254, 1316, 1362
Sau3AI	GATC	4 cut(s)	330, 556, 599, 950
Sau96I	GGNCC	1 cut(s)	628
SchI	GAGTC	2 cut(s)	471, 525
ScrFI	CCNGG	3 cut(s)	542, 768, 945
SduI	GDGCHC	3 cut(s)	930, 1069, 1181
SfaNI	GCATC	6 cut(s)	83, 457, 961, 1017, 1205, 1327
SmiMI	CAYNNNNRTG	3 cut(s)	92, 794, 1224
Sse9I	AATT	3 cut(s)	14, 37, 1294
SseBI	AGGCCT	1 cut(s)	540
SsiI	CCGC	3 cut(s)	290, 421, 1254
SspI	AATATT	1 cut(s)	1132
SspMI	CTAG	3 cut(s)	356, 444, 1193
SstI	GAGCTC	1 cut(s)	1069
StuI	AGGCCT	1 cut(s)	540
StyD4I	CCNGG	3 cut(s)	540, 766, 943
StyI	CCWWGG	1 cut(s)	1020
TaaI	ACNGT	5 cut(s)	397, 498, 707, 859, 1015
TaiI	ACGT	1 cut(s)	870
TaqI	TCGA	3 cut(s)	465, 665, 1063
TasI	AATT	3 cut(s)	14, 37, 1294
TatI	WGTACW	1 cut(s)	475
TauI	GCSGC	1 cut(s)	1256
TfiI	GAWTC	5 cut(s)	196, 401, 906, 940, 1142
Tru1I	TTAA	4 cut(s)	17, 260, 428, 830
Tru9I	TTAA	4 cut(s)	17, 260, 428, 830
TscAI	CASTG	2 cut(s)	402, 985
TseFI	GTSAC	2 cut(s)	391, 787
TseI	GCWGC	7 cut(s)	343, 362, 457, 614, 1030, 1315, 1361
Tsp45I	GTSAC	2 cut(s)	391, 787
TspDTI	ATGAA	3 cut(s)	124, 597, 699
TspGWI	ACGGA	1 cut(s)	206
TspRI	CASTG	2 cut(s)	402, 985
VneI	GTGCAC	1 cut(s)	1177
XapI	RAATTY	2 cut(s)	37, 1294
XceI	RCATGY	1 cut(s)	887
XmiI	GTMKAC	1 cut(s)	571
XmnI	GAANNNNTTC	1 cut(s)	799
XspI	CTAG	3 cut(s)	356, 444, 1193
Zsp2I	ATGCAT	2 cut(s)	76, 976

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.