Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0008959

Physical Location & Seq

Genomic Coordinates Reverse (-)

9178815 .. 9183880

Gene Structure 5066 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1119000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2103 bp

ATGTCTGCTTTTGAAGGAGTTATTGTTTCTGATACATGGCTGCAGAGCCAATTCACTCAAGTCGAGCTTCGCACCCTCAAATCGAAATTTCTTTCGATAAGGAATCAGTCTGGTCGAGTCACATTGGGAGATTTGCCGCCTGTTTTTGCGAAATTGAAGGCTTTCAATGATATGTTCAATGAAGATGAGATTAAATCAATCTTATTGGAGTCGGGCAGTAACATTGGTGAAGAAATTGATTTTGAGTCCTATCTTCGGGCATATTTGAATTTGCAAGCGCGTGAAAATGCAAGATCTGGTGGTTCAAAACAATCTTCCTTTCTCAAGGCAACCACAACAACGGTTCACCATGCGATTAATACTTCTGAGAAGTCTTCTTATGTTGCTCACATTAACGGCTATCTAGCGGAAGATCCGTTCTTGAAGAAGTATCTTCCTTTAGATCCATCTACAAATGCTCTATTTGATCTTGCAAAAGACGGAGTACTACTCTGTAAGCTTATCAATATAGCTGTTCCTGGGACTATAGATGAGCGAGCTATCAACACTAAAGCAGTTCTTAATCCATGGGAGAGGAATGAGAACCATACCCTTTGTCTTAATTCTGCTAAGGCTATCGGCTGTACTGTAGTTAACATCGGCACACAGGATTTGGTTGAAGCAAGACCCCATCTTGTACTTGGCTTGATTTCACAAATAATCAAGATTCAACTGTTAGCTGATCTTAATCTGAAGAAAACTCCACAACTTGTGGAATTGGTGGATGACAGCAAGGATGTGGAAGAGCTCTTGGGTCTACCACCCGAGAAAGTTCTACTAAAATGGATGAATTTTCATCTAAAGAAAGCTGGCTACGAAAAGCAAGTCACAAACTTCTCTTCTGATGTGAAGGATGGAGAGGCTTATGCTTACCTGCTTAATGCTCTTGCACCAGAGCACTCAGGCCCTGCTGCCTTGAATATGAAAGATCCTACAAAAAGAGCAAACCTGGTTCTTGAGCAAGCAGAGAAATTGGATTGCAAAAGATACCTTACTCCAAAGGACATTGTTGAGGGCTCACCAAATCTTAATCTTGCATTTGTTGCACAAATTTTCCAGCACAGAAATGGGTTGTCTGCTGATTGTAAAAAAATGTCCTTTGCTGAGATGATGACAGATGATGCCGAAACTTCTCGAGAAGAGAGATGTTTCCGACTGTGGATTAACAGTCTTGGAACTGCTACCTATGTCAATAATCTTTTTGAAGATGTCAGAAATGGATGGGTTCTTTTAGAAGTGCTTGACAAAATTTCTTCAGGATCAGTCAACTGGAAGAAGGCATCAAAGCCTCCAATAAAAATGCCGTTTAGAAAAGTCGAGAATGCCAACCAAGTTATAGAGATTGGAAAGGAATTAAATTTCTCCCTTGTGAATGTAGCAGGGAATGATATTGTGCAAGGAAATAAGAAGCTCATACTGGCATTTTTATGGCAGTTGATGAGGTTTAGTATGCTTCAACTCTTGAGAAATTTGAGATCCCACTCCCAAGATAAAGCGGGTAAAGAGATTACTGATGCTGACATTCTAAACTGGGCGAACAAAAAAGTTAAGAAGGCGGGTAGAACCTCACAAATAGAGAGTTTCAAGGATAAAAACCTTTCAAGTGGAGTTTTCTTCCTCGAGCTTCTTAGTTCTGTGGAGCCAAGGGTGGTCAACTGGAGCCTTGCCACCAAGGGGGAAACTGAGGAAGATAAGAAGTTGAATGCAACGTATATAATCAGTGTTGCCCGAAAGCTAGGTTGCTCCATTTTCTTGTTACCAGAGGACATTATGGTGGTAAACCAGAAGATGATGCTTACTTTGACAGCAAGCATCATGTACTGGAGTCTGCAGCAACCGGGAGGGGAAGCGGAATCAAACCTTACCACTCCTGACGGGTCCCCTACAGCTTCCGCTGATGGCGAGAAGGAAGCAACTTTGGCCAGTGCAGTCTCCAATCTGTCTATGGACGAAGCTGCTTCAAATCCAACCTACCAACAGACAGAACACGAGAATCCCTCGAAGGTCGAAAATGAGGAATCGTCTGTAGAAAATGAAAAAAAGGACACTTCTGGTGAAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0005884 GO:0006996 GO:0007010 GO:0007015 GO:0008092 GO:0008150 GO:0009653 GO:0009826 GO:0009846 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0015629 GO:0016043 GO:0016049 GO:0022414 GO:0022607 GO:0030029 GO:0030036 GO:0030154 GO:0030312 GO:0032432 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044706 GO:0044877 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0051015 GO:0051017 GO:0051639 GO:0051704 GO:0051764 GO:0060560 GO:0061572 GO:0071840 GO:0071944 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Protein Analysis

701

Amino Acids

77.87

Weight (kDa)

5.72

Isoelectric Point (pI)

37.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CH	PF00307	152 - 235	3e-15	Calponin homology (CH) domain
CH	PF00307	267 - 368	1.1e-23	Calponin homology (CH) domain
CH	PF00307	393 - 495	3.2e-18	Calponin homology (CH) domain
CH	PF00307	518 - 620	1.3e-18	Calponin homology (CH) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008959)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G04750 AT5G35700
fragaria_vesca	FvH4_6g41540 FvH4_6g41540
malus_domestica	MD09G1119000.v1.1 MD17G1109900.v1.1
prunus_persica	Prupe.3G205200_v2.0.a1
pyrus_communis	pycom09g04390 pycom17g10220
rosa_chinensis	RchiOBHm_Chr2g0157211
rosa_laevigata	RLG00000020972
rosa_multiflora	Rmu_sc0006282.1_g000007 Rmu_sc0039256.1_g000001
rosa_roxburghii	Rroxscaffold_2G00092410
rosa_rugosa	Rorug02G0458400 Rorug02G0458500
rosa_samantha	Rh2AG523900 Rh2BG538300 Rh2CG508700 Rh2DG547200
rosa_wichuraiana	Rw2G043330 Rw2G043390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	921
AccI	GTMKAC	1 cut(s)	796
AccII	CGCG	1 cut(s)	280
AciI	CCGC	6 cut(s)	137, 407, 1535, 1595, 1889, 1932
AclWI	GGATC	5 cut(s)	407, 437, 962, 1306, 1509
AcoI	YGGCCR	1 cut(s)	1959
AcsI	RAATTY	7 cut(s)	86, 268, 829, 1089, 1287, 1396, 1507
AcuI	CTGAAG	2 cut(s)	752, 1278
AfaI	GTAC	4 cut(s)	486, 625, 678, 1859
AfiI	CCNNNNNNNGG	2 cut(s)	255, 1713
AjnI	CCWGG	2 cut(s)	517, 987
AloI	GAACNNNNNNTCC	2 cut(s)	401, 433
Alw21I	GWGCWC	2 cut(s)	789, 939
Alw26I	GTCTC	1 cut(s)	1975
AlwI	GGATC	5 cut(s)	407, 437, 962, 1306, 1509
AlwNI	CAGNNNCTG	1 cut(s)	947
Ama87I	CYCGRG	3 cut(s)	803, 1173, 1658
AoxI	GGCC	2 cut(s)	943, 1959
ApeKI	GCWGC	4 cut(s)	40, 950, 1870, 1994
ApoI	RAATTY	7 cut(s)	86, 268, 829, 1089, 1287, 1396, 1507
ArsI	GACNNNNNNTTYG	2 cut(s)	849, 881
AseI	ATTAAT	1 cut(s)	357
Asp700I	GAANNNNTTC	1 cut(s)	161
AspLEI	GCGC	1 cut(s)	280
AspS9I	GGNCC	2 cut(s)	944, 1917
AsuC2I	CCSGG	1 cut(s)	1878
AsuHPI	GGTGA	3 cut(s)	239, 338, 1050
AvaI	CYCGRG	3 cut(s)	803, 1173, 1658
AvaII	GGWCC	1 cut(s)	1917
BalI	TGGCCA	1 cut(s)	1961
BanII	GRGCYC	2 cut(s)	789, 1058
BauI	CACGAG	1 cut(s)	2027
BbsI	GAAGAC	1 cut(s)	366
Bbv12I	GWGCWC	2 cut(s)	789, 939
BbvI	GCAGC	4 cut(s)	27, 937, 1882, 1981
BccI	CCATC	5 cut(s)	454, 678, 887, 1254, 1931
BceAI	ACGGC	2 cut(s)	412, 1327
BciT130I	CCWGG	2 cut(s)	519, 989
BcnI	CCSGG	1 cut(s)	1878
BcoDI	GTCTC	1 cut(s)	1975
BfaI	CTAG	2 cut(s)	404, 1775
BfmI	CTRYAG	6 cut(s)	41, 525, 627, 1868, 1923, 2064
BfuAI	ACCTGC	1 cut(s)	921
BglII	AGATCT	1 cut(s)	293
BisI	GCNGC	5 cut(s)	41, 137, 951, 1871, 1995
BlsI	GCNGC	5 cut(s)	42, 138, 952, 1872, 1996
BmcAI	AGTACT	1 cut(s)	486
Bme1390I	CCNGG	3 cut(s)	519, 989, 1878
Bme18I	GGWCC	1 cut(s)	1917
BmeT110I	CYCGRG	3 cut(s)	803, 1173, 1658
BmgT120I	GGNCC	2 cut(s)	944, 1917
BmiI	GGNNCC	4 cut(s)	1680, 1700, 1918, 1919
BmrFI	CCNGG	3 cut(s)	519, 989, 1878
BmrI	ACTGGG	1 cut(s)	1579
BmsI	GCATC	5 cut(s)	1150, 1328, 1543, 1821, 1860
BmuI	ACTGGG	1 cut(s)	1579
BpiI	GAAGAC	1 cut(s)	366
BplI	GAGNNNNNCTC	4 cut(s)	474, 506, 2021, 2053
BpmI	CTGGAG	2 cut(s)	1717, 1882
Bpu10I	CCTNAGC	1 cut(s)	609
BpuEI	CTTGAG	4 cut(s)	42, 308, 1016, 1522
BpuMI	CCSGG	1 cut(s)	1878
BsaBI	GATNNNNATC	1 cut(s)	726
BsaJI	CCNNGG	4 cut(s)	518, 566, 1682, 1710
BsaXI	ACNNNNNCTCC	2 cut(s)	9, 39
Bsc4I	CCNNNNNNNGG	2 cut(s)	255, 1713
Bse1I	ACTGG	6 cut(s)	1313, 1461, 1574, 1700, 1865, 1962
Bse8I	GATNNNNATC	1 cut(s)	726
BseBI	CCWGG	2 cut(s)	519, 989
BseDI	CCNNGG	4 cut(s)	518, 566, 1682, 1710
BseGI	GGATG	5 cut(s)	769, 781, 831, 898, 1265
BseJI	GATNNNNATC	1 cut(s)	726
BseLI	CCNNNNNNNGG	2 cut(s)	255, 1713
BseMII	CTCAG	4 cut(s)	357, 954, 1134, 1713
BseNI	ACTGG	6 cut(s)	1313, 1461, 1574, 1700, 1865, 1962
BseXI	GCAGC	4 cut(s)	27, 937, 1882, 1981
BsgI	GTGCAG	1 cut(s)	1986
Bsh1236I	CGCG	1 cut(s)	280
BshFI	GGCC	2 cut(s)	945, 1961
BsiHKAI	GWGCWC	2 cut(s)	789, 939
BsiHKCI	CYCGRG	3 cut(s)	803, 1173, 1658
BsiSI	CCGG	1 cut(s)	1877
BslFI	GGGAC	2 cut(s)	535, 1903
BslI	CCNNNNNNNGG	2 cut(s)	255, 1713
BsmAI	GTCTC	1 cut(s)	1975
BsmFI	GGGAC	2 cut(s)	535, 1903
BsmI	GAATGC	2 cut(s)	1366, 1747
BsnI	GGCC	2 cut(s)	945, 1961
BsoBI	CYCGRG	3 cut(s)	803, 1173, 1658
Bsp1286I	GDGCHC	3 cut(s)	789, 939, 1058
Bsp143I	GATC	8 cut(s)	293, 412, 442, 466, 721, 967, 1298, 1514
Bsp19I	CCATGG	1 cut(s)	566
BspACI	CCGC	6 cut(s)	137, 407, 1535, 1595, 1889, 1932
BspANI	GGCC	2 cut(s)	945, 1961
BspCNI	CTCAG	4 cut(s)	358, 953, 1135, 1714
BspFNI	CGCG	1 cut(s)	280
BspLI	GGNNCC	4 cut(s)	1680, 1700, 1918, 1919
BspMAI	CTGCAG	2 cut(s)	45, 1872
BspMI	ACCTGC	1 cut(s)	921
BspPI	GGATC	5 cut(s)	407, 437, 962, 1306, 1509
BspQI	GCTCTTC	1 cut(s)	777
BsrI	ACTGG	6 cut(s)	1313, 1461, 1574, 1700, 1865, 1962
BssECI	CCNNGG	4 cut(s)	518, 566, 1682, 1710
BssMI	GATC	8 cut(s)	293, 412, 442, 466, 721, 967, 1298, 1514
BssSI	CACGAG	1 cut(s)	2027
BssT1I	CCWWGG	3 cut(s)	566, 1682, 1710
Bst2BI	CACGAG	1 cut(s)	2027
Bst2UI	CCWGG	2 cut(s)	519, 989
Bst4CI	ACNGT	5 cut(s)	343, 628, 714, 1197, 1208
Bst6I	CTCTTC	3 cut(s)	777, 883, 1173
BstAPI	GCANNNNNTGC	1 cut(s)	1082
BstC8I	GCNNGC	5 cut(s)	276, 537, 850, 1002, 1849
BstDEI	CTNAG	6 cut(s)	366, 609, 940, 1143, 1667, 1722
BstDSI	CCRYGG	1 cut(s)	566
BstF5I	GGATG	5 cut(s)	769, 781, 831, 898, 1265
BstFNI	CGCG	1 cut(s)	280
BstHHI	GCGC	1 cut(s)	280
BstKTI	GATC	8 cut(s)	296, 415, 445, 469, 724, 970, 1301, 1517
BstMAI	GTCTC	1 cut(s)	1975
BstMBI	GATC	8 cut(s)	293, 412, 442, 466, 721, 967, 1298, 1514
BstMWI	GCNNNNNNNGC	2 cut(s)	1082, 1958
BstNI	CCWGG	2 cut(s)	519, 989
BstSCI	CCNGG	3 cut(s)	517, 987, 1876
BstSFI	CTRYAG	6 cut(s)	41, 525, 627, 1868, 1923, 2064
BstUI	CGCG	1 cut(s)	280
BstV1I	GCAGC	4 cut(s)	27, 937, 1882, 1981
BstV2I	GAAGAC	1 cut(s)	366
BstX2I	RGATCY	5 cut(s)	293, 412, 442, 967, 1514
BstYI	RGATCY	5 cut(s)	293, 412, 442, 967, 1514
BsuRI	GGCC	2 cut(s)	945, 1961
BtgI	CCRYGG	1 cut(s)	566
BtsCI	GGATG	5 cut(s)	769, 781, 831, 898, 1265
BtsIMutI	CAGTG	2 cut(s)	1765, 1969
BveI	ACCTGC	1 cut(s)	921
Cac8I	GCNNGC	5 cut(s)	276, 537, 850, 1002, 1849
CaiI	CAGNNNCTG	1 cut(s)	947
CfoI	GCGC	1 cut(s)	280
Cfr13I	GGNCC	2 cut(s)	944, 1917
CsiI	ACCWGGT	1 cut(s)	987
Csp6I	GTAC	4 cut(s)	485, 624, 677, 1858
CviAII	CATG	4 cut(s)	36, 350, 567, 1855
CviQI	GTAC	4 cut(s)	485, 624, 677, 1858
DdeI	CTNAG	6 cut(s)	366, 609, 940, 1143, 1667, 1722
DpnI	GATC	8 cut(s)	295, 414, 444, 468, 723, 969, 1300, 1516
DpnII	GATC	8 cut(s)	293, 412, 442, 466, 721, 967, 1298, 1514
EaeI	YGGCCR	1 cut(s)	1959
Eam1104I	CTCTTC	3 cut(s)	777, 883, 1173
EarI	CTCTTC	3 cut(s)	777, 883, 1173
Ecl136II	GAGCTC	1 cut(s)	787
Eco130I	CCWWGG	3 cut(s)	566, 1682, 1710
Eco24I	GRGCYC	2 cut(s)	789, 1058
Eco47I	GGWCC	1 cut(s)	1917
Eco53kI	GAGCTC	1 cut(s)	787
Eco57I	CTGAAG	2 cut(s)	752, 1278
Eco88I	CYCGRG	3 cut(s)	803, 1173, 1658
EcoICRI	GAGCTC	1 cut(s)	787
EcoO109I	RGGNCCY	2 cut(s)	944, 1917
EcoRII	CCWGG	2 cut(s)	517, 987
EcoT14I	CCWWGG	3 cut(s)	566, 1682, 1710
EcoT38I	GRGCYC	2 cut(s)	789, 1058
ErhI	CCWWGG	3 cut(s)	566, 1682, 1710
FaeI	CATG	4 cut(s)	39, 353, 570, 1858
FalI	AAGNNNNNCTT	4 cut(s)	51, 83, 543, 575
FaqI	GGGAC	2 cut(s)	535, 1903
FatI	CATG	4 cut(s)	35, 349, 566, 1854
FauI	CCCGC	2 cut(s)	1528, 1588
FblI	GTMKAC	1 cut(s)	796
Fnu4HI	GCNGC	5 cut(s)	41, 137, 951, 1871, 1995
FokI	GGATG	5 cut(s)	776, 788, 838, 905, 1272
FriOI	GRGCYC	2 cut(s)	789, 1058
Fsp4HI	GCNGC	5 cut(s)	41, 137, 951, 1871, 1995
FspBI	CTAG	2 cut(s)	404, 1775
GlaI	GCGC	1 cut(s)	279
GluI	GCNGC	5 cut(s)	41, 137, 951, 1871, 1995
GsuI	CTGGAG	2 cut(s)	1717, 1882
HaeIII	GGCC	2 cut(s)	945, 1961
HapII	CCGG	1 cut(s)	1877
HhaI	GCGC	1 cut(s)	280
Hin1II	CATG	4 cut(s)	39, 353, 570, 1858
Hin6I	GCGC	1 cut(s)	278
HinP1I	GCGC	1 cut(s)	278
HincII	GTYRAC	3 cut(s)	634, 1306, 1693
HindII	GTYRAC	3 cut(s)	634, 1306, 1693
HindIII	AAGCTT	1 cut(s)	497
HinfI	GANTC	9 cut(s)	103, 117, 209, 245, 706, 1864, 1892, 2032, 2057
HpaI	GTTAAC	1 cut(s)	634
HpaII	CCGG	1 cut(s)	1877
HphI	GGTGA	3 cut(s)	239, 338, 1050
Hpy166II	GTNNAC	6 cut(s)	346, 634, 797, 1306, 1693, 1819
Hpy188I	TCNGA	6 cut(s)	31, 367, 732, 883, 1193, 1253
Hpy188III	TCNNGA	9 cut(s)	421, 703, 995, 1173, 1175, 1296, 1357, 1501, 1910
Hpy8I	GTNNAC	6 cut(s)	346, 634, 797, 1306, 1693, 1819
HpyAV	CCTTC	7 cut(s)	8, 151, 883, 1309, 1585, 1939, 2035
HpyCH4III	ACNGT	5 cut(s)	343, 628, 714, 1197, 1208
HpyCH4IV	ACGT	1 cut(s)	1748
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1082, 1958
HpyF3I	CTNAG	6 cut(s)	366, 609, 940, 1143, 1667, 1722
HpySE526I	ACGT	1 cut(s)	1748
Hsp92II	CATG	4 cut(s)	39, 353, 570, 1858
HspAI	GCGC	1 cut(s)	278
KflI	GGGWCCC	1 cut(s)	1917
KspAI	GTTAAC	1 cut(s)	634
Kzo9I	GATC	8 cut(s)	293, 412, 442, 466, 721, 967, 1298, 1514
LguI	GCTCTTC	1 cut(s)	777
LmnI	GCTCC	3 cut(s)	1678, 1698, 1787
Lsp1109I	GCAGC	4 cut(s)	27, 937, 1882, 1981
LweI	GCATC	5 cut(s)	1150, 1328, 1543, 1821, 1860
MabI	ACCWGGT	1 cut(s)	987
MaeI	CTAG	2 cut(s)	404, 1775
MaeII	ACGT	1 cut(s)	1748
MaeIII	GTNAC	4 cut(s)	118, 218, 865, 1794
MalI	GATC	8 cut(s)	295, 414, 444, 468, 723, 969, 1300, 1516
MboI	GATC	8 cut(s)	293, 412, 442, 466, 721, 967, 1298, 1514
MflI	RGATCY	5 cut(s)	293, 412, 442, 967, 1514
MhlI	GDGCHC	3 cut(s)	789, 939, 1058
MlsI	TGGCCA	1 cut(s)	1961
MluNI	TGGCCA	1 cut(s)	1961
MlyI	GAGTC	4 cut(s)	126, 218, 254, 1873
MmeI	TCCRAC	2 cut(s)	1216, 2030
Mox20I	TGGCCA	1 cut(s)	1961
MroXI	GAANNNNTTC	1 cut(s)	161
MscI	TGGCCA	1 cut(s)	1961
MslI	CAYNNNNRTG	3 cut(s)	1104, 1465, 1811
Msp20I	TGGCCA	1 cut(s)	1961
MspA1I	CMGCKG	1 cut(s)	1934
MspI	CCGG	1 cut(s)	1877
MspR9I	CCNGG	3 cut(s)	519, 989, 1878
Mva1269I	GAATGC	2 cut(s)	1366, 1747
MvaI	CCWGG	2 cut(s)	519, 989
MvnI	CGCG	1 cut(s)	280
MwoI	GCNNNNNNNGC	2 cut(s)	1082, 1958
NciI	CCSGG	1 cut(s)	1878
NcoI	CCATGG	1 cut(s)	566
NdeII	GATC	8 cut(s)	293, 412, 442, 466, 721, 967, 1298, 1514
NlaIII	CATG	4 cut(s)	39, 353, 570, 1858
NlaIV	GGNNCC	4 cut(s)	1680, 1700, 1918, 1919
NmuCI	GTSAC	2 cut(s)	118, 865
PaeR7I	CTCGAG	2 cut(s)	1173, 1658
PciSI	GCTCTTC	1 cut(s)	777
PctI	GAATGC	2 cut(s)	1366, 1747
PdmI	GAANNNNTTC	1 cut(s)	161
PfeI	GAWTC	5 cut(s)	103, 706, 1892, 2032, 2057
PkrI	GCNGC	5 cut(s)	42, 138, 952, 1872, 1996
PleI	GAGTC	4 cut(s)	125, 217, 253, 1872
PpsI	GAGTC	4 cut(s)	125, 217, 253, 1872
PpuMI	RGGWCCY	1 cut(s)	1917
PshBI	ATTAAT	1 cut(s)	357
Psp124BI	GAGCTC	1 cut(s)	789
Psp5II	RGGWCCY	1 cut(s)	1917
Psp6I	CCWGG	2 cut(s)	517, 987
PspGI	CCWGG	2 cut(s)	517, 987
PspN4I	GGNNCC	4 cut(s)	1680, 1700, 1918, 1919
PspPI	GGNCC	2 cut(s)	944, 1917
PspPPI	RGGWCCY	1 cut(s)	1917
PspXI	VCTCGAGB	1 cut(s)	1658
PstI	CTGCAG	2 cut(s)	45, 1872
PstNI	CAGNNNCTG	1 cut(s)	947
PsuI	RGATCY	5 cut(s)	293, 412, 442, 967, 1514
RsaI	GTAC	4 cut(s)	486, 625, 678, 1859
RsaNI	GTAC	4 cut(s)	485, 624, 677, 1858
RseI	CAYNNNNRTG	3 cut(s)	1104, 1465, 1811
SacI	GAGCTC	1 cut(s)	789
SapI	GCTCTTC	1 cut(s)	777
SatI	GCNGC	5 cut(s)	41, 137, 951, 1871, 1995
Sau3AI	GATC	8 cut(s)	293, 412, 442, 466, 721, 967, 1298, 1514
Sau96I	GGNCC	2 cut(s)	944, 1917
ScaI	AGTACT	1 cut(s)	486
SchI	GAGTC	4 cut(s)	126, 218, 254, 1873
ScrFI	CCNGG	3 cut(s)	519, 989, 1878
SduI	GDGCHC	3 cut(s)	789, 939, 1058
SexAI	ACCWGGT	1 cut(s)	987
SfaNI	GCATC	5 cut(s)	1150, 1328, 1543, 1821, 1860
SfcI	CTRYAG	6 cut(s)	41, 525, 627, 1868, 1923, 2064
Sfr274I	CTCGAG	2 cut(s)	1173, 1658
SinI	GGWCC	1 cut(s)	1917
SlaI	CTCGAG	2 cut(s)	1173, 1658
SmiMI	CAYNNNNRTG	3 cut(s)	1104, 1465, 1811
SmlI	CTYRAG	6 cut(s)	57, 323, 995, 1173, 1501, 1658
SmoI	CTYRAG	6 cut(s)	57, 323, 995, 1173, 1501, 1658
SsiI	CCGC	6 cut(s)	137, 407, 1535, 1595, 1889, 1932
SspMI	CTAG	2 cut(s)	404, 1775
SstI	GAGCTC	1 cut(s)	789
StyD4I	CCNGG	3 cut(s)	517, 987, 1876
StyI	CCWWGG	3 cut(s)	566, 1682, 1710
TaaI	ACNGT	5 cut(s)	343, 628, 714, 1197, 1208
TaiI	ACGT	1 cut(s)	1751
TaqI	TCGA	9 cut(s)	63, 83, 95, 115, 1174, 1356, 1659, 2039, 2046
TatI	WGTACW	4 cut(s)	484, 623, 676, 1857
TauI	GCSGC	1 cut(s)	139
TfiI	GAWTC	5 cut(s)	103, 706, 1892, 2032, 2057
TscAI	CASTG	2 cut(s)	1765, 1969
TseFI	GTSAC	2 cut(s)	118, 865
TseI	GCWGC	4 cut(s)	40, 950, 1870, 1994
Tsp45I	GTSAC	2 cut(s)	118, 865
TspDTI	ATGAA	5 cut(s)	195, 824, 842, 977, 2088
TspGWI	ACGGA	2 cut(s)	405, 495
TspRI	CASTG	2 cut(s)	1765, 1969
VpaK11BI	GGWCC	1 cut(s)	1917
VspI	ATTAAT	1 cut(s)	357
XapI	RAATTY	7 cut(s)	86, 268, 829, 1089, 1287, 1396, 1507
XcmI	CCANNNNNNNNNTGG	3 cut(s)	677, 1103, 1981
XhoI	CTCGAG	2 cut(s)	1173, 1658
XmiI	GTMKAC	1 cut(s)	796
XmnI	GAANNNNTTC	1 cut(s)	161
XspI	CTAG	2 cut(s)	404, 1775
ZrmI	AGTACT	1 cut(s)	486

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.