MD09G1135800.v1.1

Rhodanese-like domain-containing protein 19

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0013561
Physical Location & Seq
Forward (+)
10429650 .. 10431193
1544 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1135800.v1.1.491

Sequence Viewer

Length: 408 bp
ATGGCTGCCATGAAAGGGCCTGAAGACGTTGCAAGTGTCGACGTCTATACAGCAAAAGGTCTCCTCAGTATAGGTCACCGTTATCTGGATGTCAGGACGATTGAGGAGTTCAACAAGAGCCACGTTGAAAATGCCATGAACATTCCTTTCATGCTCATCACAGAAGAAGGTAGAGTGAAAAACCCTGAATTTCTCACCCAAATTTCCTCAACGCTCAAGAAAGAGGATCACTTGGTCGTGGGTTGCAACAGTGGAGGAAGAGCACTTAAAGCTTGTGTTGATCTTCTTAATGAAGGTTTCCAGCATGTGACCAACATGGAAGGAGGCTACTCCTCTTGGGTGGACAGTGGACTTGCCGGTGACAAACCCTCCGACGAGTTGAAAGTCGCTTGCAAGTTCCGCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009636 GO:0016491 GO:0030611 GO:0042221 GO:0046685 GO:0050896 GO:0055114 GO:0071722 GO:0098754
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhodanese

Protein Analysis

136

Amino Acids

14.8

Weight (kDa)

5.54

Isoelectric Point (pI)

32.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 28 - 115 3.3e-16 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013561)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21045
fragaria_vesca FvH4_6g39880 FvH4_6g39880
malus_domestica MD09G1135800.v1.1
prunus_persica Prupe.3G190900_v2.0.a1
pyrus_communis pycom09g05680
rosa_chinensis RchiOBHm_Chr2g0154401
rosa_laevigata RLG00000020775
rosa_multiflora Rmu_sc0003808.1_g000009 Rmu_sc0025687.1_g000004 Rmu_sc0032891.1_g000001
rosa_roxburghii Rroxscaffold_2G00094360
rosa_rugosa Rorug02G0444200
rosa_samantha Rh2AG508500 Rh2BG519800 Rh2CG494000 Rh2DG530000
rosa_wichuraiana Rw2G041760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 45
AccI GTMKAC 1 cut(s) 39
AciI CCGC 1 cut(s) 400
AclWI GGATC 1 cut(s) 234
AcsI RAATTY 2 cut(s) 188, 201
AcuI CTGAAG 1 cut(s) 42
AcyI GRCGYC 1 cut(s) 42
AfiI CCNNNNNNNGG 2 cut(s) 15, 85
AgsI TTSAA 3 cut(s) 112, 128, 382
AluBI AGCT 1 cut(s) 272
AluI AGCT 1 cut(s) 272
Alw21I GWGCWC 1 cut(s) 265
Alw26I GTCTC 1 cut(s) 65
AlwI GGATC 1 cut(s) 234
AoxI GGCC 1 cut(s) 17
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 2 cut(s) 188, 201
AspS9I GGNCC 1 cut(s) 17
AsuHPI GGTGA 3 cut(s) 68, 187, 371
BbsI GAAGAC 1 cut(s) 30
Bbv12I GWGCWC 1 cut(s) 265
BcoDI GTCTC 1 cut(s) 65
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
BmgT120I GGNCC 1 cut(s) 17
BpiI GAAGAC 1 cut(s) 30
BpuEI CTTGAG 1 cut(s) 200
BsaHI GRCGYC 1 cut(s) 42
BsaI GGTCTC 1 cut(s) 65
BsaXI ACNNNNNCTCC 2 cut(s) 353, 383
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 85
Bse118I RCCGGY 1 cut(s) 356
BseGI GGATG 1 cut(s) 94
BseLI CCNNNNNNNGG 2 cut(s) 15, 85
BseMII CTCAG 1 cut(s) 79
BseRI GAGGAG 3 cut(s) 53, 119, 322
BshFI GGCC 1 cut(s) 19
BsiHKAI GWGCWC 1 cut(s) 265
BsiSI CCGG 1 cut(s) 357
BslI CCNNNNNNNGG 2 cut(s) 15, 85
BsmAI GTCTC 1 cut(s) 65
BsnI GGCC 1 cut(s) 19
Bso31I GGTCTC 1 cut(s) 65
Bsp1286I GDGCHC 1 cut(s) 265
Bsp143I GATC 2 cut(s) 226, 280
BspACI CCGC 1 cut(s) 400
BspANI GGCC 1 cut(s) 19
BspCNI CTCAG 1 cut(s) 78
BspPI GGATC 1 cut(s) 234
BspQI GCTCTTC 1 cut(s) 253
BspTNI GGTCTC 1 cut(s) 65
BsrFI RCCGGY 1 cut(s) 356
BssAI RCCGGY 1 cut(s) 356
BssMI GATC 2 cut(s) 226, 280
BssNI GRCGYC 1 cut(s) 42
Bst4CI ACNGT 3 cut(s) 80, 251, 347
Bst6I CTCTTC 1 cut(s) 253
BstACI GRCGYC 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 391
BstDEI CTNAG 1 cut(s) 65
BstEII GGTNACC 1 cut(s) 74
BstF5I GGATG 1 cut(s) 94
BstKTI GATC 2 cut(s) 229, 283
BstMAI GTCTC 1 cut(s) 65
BstMBI GATC 2 cut(s) 226, 280
BstMWI GCNNNNNNNGC 2 cut(s) 269, 399
BstNSI RCATGY 1 cut(s) 308
BstPI GGTNACC 1 cut(s) 74
BstV2I GAAGAC 1 cut(s) 30
BsuRI GGCC 1 cut(s) 19
BtsCI GGATG 1 cut(s) 94
BtsIMutI CAGTG 2 cut(s) 256, 352
Cac8I GCNNGC 1 cut(s) 391
Cfr10I RCCGGY 1 cut(s) 356
Cfr13I GGNCC 1 cut(s) 17
CviAII CATG 5 cut(s) 10, 136, 151, 305, 316
CviJI RGCY 5 cut(s) 5, 19, 120, 272, 327
CviKI_1 RGCY 5 cut(s) 5, 19, 120, 272, 327
DdeI CTNAG 1 cut(s) 65
DpnI GATC 2 cut(s) 228, 282
DpnII GATC 2 cut(s) 226, 280
Eam1104I CTCTTC 1 cut(s) 253
EarI CTCTTC 1 cut(s) 253
EciI GGCGGA 1 cut(s) 389
Eco31I GGTCTC 1 cut(s) 65
Eco57I CTGAAG 1 cut(s) 42
Eco91I GGTNACC 1 cut(s) 74
EcoO109I RGGNCCY 1 cut(s) 17
EcoO65I GGTNACC 1 cut(s) 74
FaeI CATG 5 cut(s) 13, 139, 154, 308, 319
FaiI YATR 7 cut(s) 11, 48, 71, 137, 152, 306, 317
FatI CATG 5 cut(s) 9, 135, 150, 304, 315
FblI GTMKAC 1 cut(s) 39
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 1 cut(s) 101
Fsp4HI GCNGC 1 cut(s) 6
GluI GCNGC 1 cut(s) 6
HaeIII GGCC 1 cut(s) 19
HapII CCGG 1 cut(s) 357
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 5 cut(s) 13, 139, 154, 308, 319
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 270
HpaII CCGG 1 cut(s) 357
HphI GGTGA 3 cut(s) 68, 187, 371
Hpy166II GTNNAC 3 cut(s) 40, 343, 350
Hpy188I TCNGA 1 cut(s) 373
Hpy188III TCNNGA 3 cut(s) 86, 94, 217
Hpy8I GTNNAC 3 cut(s) 40, 343, 350
Hpy99I CGWCG 2 cut(s) 44, 377
HpyAV CCTTC 3 cut(s) 161, 287, 314
HpyCH4III ACNGT 3 cut(s) 80, 251, 347
HpyCH4IV ACGT 3 cut(s) 27, 42, 123
HpyCH4V TGCA 3 cut(s) 32, 246, 393
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 399
HpyF3I CTNAG 1 cut(s) 65
HpySE526I ACGT 3 cut(s) 27, 42, 123
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 5 cut(s) 13, 139, 154, 308, 319
Kzo9I GATC 2 cut(s) 226, 280
LguI GCTCTTC 1 cut(s) 253
LpnPI CCDG 6 cut(s) 33, 71, 79, 198, 314, 370
MaeII ACGT 3 cut(s) 27, 42, 123
MaeIII GTNAC 3 cut(s) 74, 307, 359
MalI GATC 2 cut(s) 228, 282
MboI GATC 2 cut(s) 226, 280
MboII GAAGA 4 cut(s) 35, 176, 270, 275
MhlI GDGCHC 1 cut(s) 265
MluCI AATT 2 cut(s) 188, 201
MmeI TCCRAC 1 cut(s) 396
MnlI CCTC 8 cut(s) 74, 97, 217, 217, 248, 317, 343, 379
MseI TTAA 2 cut(s) 267, 288
MspI CCGG 1 cut(s) 357
MwoI GCNNNNNNNGC 2 cut(s) 269, 399
NdeII GATC 2 cut(s) 226, 280
NlaIII CATG 5 cut(s) 13, 139, 154, 308, 319
NmuCI GTSAC 3 cut(s) 74, 307, 359
NspI RCATGY 1 cut(s) 308
PciSI GCTCTTC 1 cut(s) 253
PkrI GCNGC 1 cut(s) 7
PspEI GGTNACC 1 cut(s) 74
PspPI GGNCC 1 cut(s) 17
SalI GTCGAC 1 cut(s) 38
SapI GCTCTTC 1 cut(s) 253
SaqAI TTAA 2 cut(s) 267, 288
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 2 cut(s) 226, 280
Sau96I GGNCC 1 cut(s) 17
SduI GDGCHC 1 cut(s) 265
SetI ASST 8 cut(s) 30, 45, 61, 76, 126, 172, 274, 298
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
Sse9I AATT 2 cut(s) 188, 201
SsiI CCGC 1 cut(s) 400
TaaI ACNGT 3 cut(s) 80, 251, 347
TaiI ACGT 3 cut(s) 30, 45, 126
TaqI TCGA 1 cut(s) 39
TasI AATT 2 cut(s) 188, 201
Tru1I TTAA 2 cut(s) 267, 288
Tru9I TTAA 2 cut(s) 267, 288
TscAI CASTG 2 cut(s) 256, 352
TseFI GTSAC 3 cut(s) 74, 307, 359
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 3 cut(s) 74, 307, 359
TspDTI ATGAA 4 cut(s) 26, 139, 152, 306
TspRI CASTG 2 cut(s) 256, 352
XapI RAATTY 2 cut(s) 188, 201
XceI RCATGY 1 cut(s) 308
XmiI GTMKAC 1 cut(s) 39
ZraI GACGTC 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.