MD09G1158500.v1.1

ATP synthase subunit epsilon

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0015125
Physical Location & Seq
Reverse (-)
12661571 .. 12664760
3190 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1158500.v1.1.491

Sequence Viewer

Length: 213 bp
ATGTCATCGAACGCAGCGGCACCGTTTTGGCGCGCAGCCGGTATGACCTACATCACCTACTCCAACATCTGTGCGAATCTGGTACGGAATTGCCTCAAGGAGCCTTACAAGACCGAAGCCATTTCTCGCGAGAGGGTCCACTTCGCCGTCTCCAAGTGGGCTGAAGGCAAGCCCGAAAAGCCCACTATTCGTACGGATACACCTGAAGCATGA

Protein Analysis

71

Amino Acids

7.88

Weight (kDa)

9.1

Isoelectric Point (pI)

37.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_Eps PF04627 9 - 56 3e-23 Mitochondrial ATP synthase epsilon chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015125)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51650
fragaria_vesca FvH4_6g37330 FvH4_6g37330
malus_domestica MD09G1158500.v1.1
prunus_persica Prupe.3G167500_v2.0.a1 Prupe.3G167500_v2.0.a1
pyrus_communis pycom09g07710 pycom17g13760
rosa_chinensis RchiOBHm_Chr2g0150171
rosa_multiflora Rmu_sc0015510.1_g000002 Rmu_ssc0000020.1_g000011
rosa_roxburghii Rroxscaffold_2G00097380
rosa_rugosa Rorug02G0419500
rosa_samantha Rh2AG478600 Rh2CG464800
rosa_wichuraiana Rw2G039060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 19
AccII CGCG 2 cut(s) 33, 129
AciI CCGC 1 cut(s) 17
AcuI CTGAAG 1 cut(s) 183
AfaI GTAC 2 cut(s) 84, 193
Alw26I GTCTC 1 cut(s) 154
ApeKI GCWGC 2 cut(s) 14, 35
AspLEI GCGC 2 cut(s) 33, 35
AspS9I GGNCC 1 cut(s) 136
AsuHPI GGTGA 1 cut(s) 46
AvaII GGWCC 1 cut(s) 136
BanI GGYRCC 1 cut(s) 19
BbvI GCAGC 2 cut(s) 26, 47
BceAI ACGGC 1 cut(s) 131
BciVI GTATCC 1 cut(s) 190
BcoDI GTCTC 1 cut(s) 154
BfuI GTATCC 1 cut(s) 190
BisI GCNGC 3 cut(s) 15, 18, 36
BlsI GCNGC 3 cut(s) 16, 19, 37
Bme18I GGWCC 1 cut(s) 136
BmgT120I GGNCC 1 cut(s) 136
BmiI GGNNCC 3 cut(s) 21, 102, 137
BpuEI CTTGAG 1 cut(s) 80
Bse118I RCCGGY 1 cut(s) 38
BsePI GCGCGC 1 cut(s) 31
BseXI GCAGC 2 cut(s) 26, 47
Bsh1236I CGCG 2 cut(s) 33, 129
BshNI GGYRCC 1 cut(s) 19
BsiSI CCGG 1 cut(s) 39
BsiWI CGTACG 1 cut(s) 191
BsmAI GTCTC 1 cut(s) 154
BsmBI CGTCTC 1 cut(s) 154
Bsp68I TCGCGA 1 cut(s) 129
BspACI CCGC 1 cut(s) 17
BspFNI CGCG 2 cut(s) 33, 129
BspLI GGNNCC 3 cut(s) 21, 102, 137
BspT107I GGYRCC 1 cut(s) 19
BsrFI RCCGGY 1 cut(s) 38
BssAI RCCGGY 1 cut(s) 38
BssHII GCGCGC 1 cut(s) 31
Bst4CI ACNGT 1 cut(s) 24
BstC8I GCNNGC 2 cut(s) 33, 170
BstFNI CGCG 2 cut(s) 33, 129
BstHHI GCGC 2 cut(s) 33, 35
BstMAI GTCTC 1 cut(s) 154
BstMWI GCNNNNNNNGC 1 cut(s) 178
BstUI CGCG 2 cut(s) 33, 129
BstV1I GCAGC 2 cut(s) 26, 47
BsuI GTATCC 1 cut(s) 190
BtuMI TCGCGA 1 cut(s) 129
Cac8I GCNNGC 2 cut(s) 33, 170
CfoI GCGC 2 cut(s) 33, 35
Cfr10I RCCGGY 1 cut(s) 38
Cfr13I GGNCC 1 cut(s) 136
Csp6I GTAC 2 cut(s) 83, 192
CviAII CATG 1 cut(s) 210
CviJI RGCY 6 cut(s) 38, 103, 119, 161, 172, 181
CviKI_1 RGCY 6 cut(s) 38, 103, 119, 161, 172, 181
CviQI GTAC 2 cut(s) 83, 192
Eco47I GGWCC 1 cut(s) 136
Eco57I CTGAAG 1 cut(s) 183
Esp3I CGTCTC 1 cut(s) 154
FaeI CATG 1 cut(s) 213
FaiI YATR 2 cut(s) 44, 211
FatI CATG 1 cut(s) 209
Fnu4HI GCNGC 3 cut(s) 15, 18, 36
Fsp4HI GCNGC 3 cut(s) 15, 18, 36
GlaI GCGC 2 cut(s) 32, 34
GluI GCNGC 3 cut(s) 15, 18, 36
HapII CCGG 1 cut(s) 39
HhaI GCGC 2 cut(s) 33, 35
Hin1II CATG 1 cut(s) 213
Hin6I GCGC 2 cut(s) 31, 33
HinP1I GCGC 2 cut(s) 31, 33
HinfI GANTC 1 cut(s) 76
HpaII CCGG 1 cut(s) 39
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 1 cut(s) 139
Hpy188III TCNNGA 1 cut(s) 128
Hpy8I GTNNAC 1 cut(s) 139
HpyAV CCTTC 1 cut(s) 158
HpyCH4III ACNGT 1 cut(s) 24
HpyF10VI GCNNNNNNNGC 1 cut(s) 178
Hsp92II CATG 1 cut(s) 213
HspAI GCGC 2 cut(s) 31, 33
LmnI GCTCC 1 cut(s) 100
LpnPI CCDG 2 cut(s) 52, 65
Lsp1109I GCAGC 2 cut(s) 26, 47
MluCI AATT 1 cut(s) 88
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 2 cut(s) 104, 126
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 1 cut(s) 39
MvnI CGCG 2 cut(s) 33, 129
MwoI GCNNNNNNNGC 1 cut(s) 178
NlaIII CATG 1 cut(s) 213
NlaIV GGNNCC 3 cut(s) 21, 102, 137
NruI TCGCGA 1 cut(s) 129
PauI GCGCGC 1 cut(s) 31
PfeI GAWTC 1 cut(s) 76
Pfl23II CGTACG 1 cut(s) 191
PkrI GCNGC 3 cut(s) 16, 19, 37
PspLI CGTACG 1 cut(s) 191
PspN4I GGNNCC 3 cut(s) 21, 102, 137
PspPI GGNCC 1 cut(s) 136
PteI GCGCGC 1 cut(s) 31
RruI TCGCGA 1 cut(s) 129
RsaI GTAC 2 cut(s) 84, 193
RsaNI GTAC 2 cut(s) 83, 192
SatI GCNGC 3 cut(s) 15, 18, 36
Sau96I GGNCC 1 cut(s) 136
SetI ASST 3 cut(s) 50, 59, 205
SinI GGWCC 1 cut(s) 136
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 1 cut(s) 88
SsiI CCGC 1 cut(s) 17
TaaI ACNGT 1 cut(s) 24
TaqI TCGA 1 cut(s) 8
TaqII GACCGA 1 cut(s) 128
TasI AATT 1 cut(s) 88
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 1 cut(s) 76
TseI GCWGC 2 cut(s) 14, 35
TspGWI ACGGA 2 cut(s) 100, 209
VpaK11BI GGWCC 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.