MD09G1158900.v1.1

GPN-loop GTPase 3 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0019764
Physical Location & Seq
Forward (+)
12706251 .. 12707742
1492 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1158900.v1.1.491

Sequence Viewer

Length: 300 bp
ATGGGTTACGCGCAGCTGGTTATTGGCCCTGCCGGCAGCGGCAAGTCGACTTATTGCTCTAGTTTGCACGAACACTGTACGGCTATTGGGAGGAGTATCAATATTGTTAATTTGGATCCTGCTGCGGAAAATTTTGATTACCCTGTGGCCATGGATATTAGGGAGCTTGTTTCCTTGGAGGATGTTATGGAGGAACTTGGTTTGGGTCCCAATGGTGGACTTTTATACTGCATGGAGTATCCTTTTTCCAAATTGTTCTCGAATTTTCTTATGTTCTTTTATTTCTTTTACTTAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ATP_bind_1

Protein Analysis

100

Amino Acids

11.08

Weight (kDa)

4.56

Isoelectric Point (pI)

44.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP_bind_1 PF03029 7 - 85 1.9e-32 Conserved hypothetical ATP binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019764)

Species Orthologous Gene IDs
malus_domestica MD09G1158900.v1.1
rosa_multiflora Rmu_sc0004191.1_g000034 Rmu_sc0009428.1_g000013 Rmu_sc0009445.1_g000005 Rmu_sc0029652.1_g000001
rosa_samantha Rh2BG490300 Rh2CG463900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 47
AccII CGCG 1 cut(s) 11
AciI CCGC 2 cut(s) 39, 125
AclWI GGATC 2 cut(s) 110, 123
AcoI YGGCCR 1 cut(s) 147
AcsI RAATTY 2 cut(s) 130, 262
AfaI GTAC 1 cut(s) 79
AfiI CCNNNNNNNGG 1 cut(s) 215
AluBI AGCT 2 cut(s) 16, 166
AluI AGCT 2 cut(s) 16, 166
AlwI GGATC 2 cut(s) 110, 123
AoxI GGCC 2 cut(s) 25, 147
ApeKI GCWGC 3 cut(s) 13, 36, 122
ApoI RAATTY 2 cut(s) 130, 262
AspLEI GCGC 1 cut(s) 13
AspS9I GGNCC 2 cut(s) 26, 206
AvaII GGWCC 1 cut(s) 206
BalI TGGCCA 1 cut(s) 149
BamHI GGATCC 1 cut(s) 115
BbvI GCAGC 3 cut(s) 25, 48, 109
BceAI ACGGC 1 cut(s) 96
BciVI GTATCC 1 cut(s) 249
BfaI CTAG 1 cut(s) 60
BfuI GTATCC 1 cut(s) 249
BglI GCCNNNNNGGC 1 cut(s) 33
BisI GCNGC 4 cut(s) 14, 37, 40, 123
BlsI GCNGC 4 cut(s) 15, 38, 41, 124
Bme18I GGWCC 1 cut(s) 206
BmgT120I GGNCC 2 cut(s) 26, 206
BmiI GGNNCC 3 cut(s) 117, 207, 208
BsaJI CCNNGG 2 cut(s) 150, 174
Bsc4I CCNNNNNNNGG 1 cut(s) 215
Bse118I RCCGGY 1 cut(s) 32
BseDI CCNNGG 2 cut(s) 150, 174
BseGI GGATG 1 cut(s) 187
BseLI CCNNNNNNNGG 1 cut(s) 215
BseRI GAGGAG 1 cut(s) 106
BseXI GCAGC 3 cut(s) 25, 48, 109
Bsh1236I CGCG 1 cut(s) 11
BshFI GGCC 2 cut(s) 27, 149
BsiSI CCGG 1 cut(s) 33
BslFI GGGAC 1 cut(s) 192
BslI CCNNNNNNNGG 1 cut(s) 215
BsmFI GGGAC 1 cut(s) 192
BsnI GGCC 2 cut(s) 27, 149
Bsp143I GATC 1 cut(s) 115
Bsp19I CCATGG 1 cut(s) 150
BspACI CCGC 2 cut(s) 39, 125
BspANI GGCC 2 cut(s) 27, 149
BspFNI CGCG 1 cut(s) 11
BspLI GGNNCC 3 cut(s) 117, 207, 208
BspPI GGATC 2 cut(s) 110, 123
BsrFI RCCGGY 1 cut(s) 32
BssAI RCCGGY 1 cut(s) 32
BssECI CCNNGG 2 cut(s) 150, 174
BssMI GATC 1 cut(s) 115
BssT1I CCWWGG 2 cut(s) 150, 174
Bst4CI ACNGT 1 cut(s) 77
BstC8I GCNNGC 1 cut(s) 34
BstDSI CCRYGG 1 cut(s) 150
BstF5I GGATG 1 cut(s) 187
BstFNI CGCG 1 cut(s) 11
BstHHI GCGC 1 cut(s) 13
BstKTI GATC 1 cut(s) 118
BstMBI GATC 1 cut(s) 115
BstMWI GCNNNNNNNGC 1 cut(s) 33
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 3 cut(s) 25, 48, 109
BstX2I RGATCY 1 cut(s) 115
BstYI RGATCY 1 cut(s) 115
BsuI GTATCC 1 cut(s) 249
BsuRI GGCC 2 cut(s) 27, 149
BtgI CCRYGG 1 cut(s) 150
BtsCI GGATG 1 cut(s) 187
BtsIMutI CAGTG 1 cut(s) 73
Cac8I GCNNGC 1 cut(s) 34
CfoI GCGC 1 cut(s) 13
Cfr10I RCCGGY 1 cut(s) 32
Cfr13I GGNCC 2 cut(s) 26, 206
Csp6I GTAC 1 cut(s) 78
CviAII CATG 2 cut(s) 151, 232
CviJI RGCY 5 cut(s) 16, 27, 83, 149, 166
CviKI_1 RGCY 5 cut(s) 16, 27, 83, 149, 166
CviQI GTAC 1 cut(s) 78
DpnI GATC 1 cut(s) 117
DpnII GATC 1 cut(s) 115
EaeI YGGCCR 1 cut(s) 147
Eco130I CCWWGG 2 cut(s) 150, 174
Eco47I GGWCC 1 cut(s) 206
EcoO109I RGGNCCY 1 cut(s) 206
EcoT14I CCWWGG 2 cut(s) 150, 174
ErhI CCWWGG 2 cut(s) 150, 174
FaeI CATG 2 cut(s) 154, 235
FaiI YATR 5 cut(s) 152, 188, 226, 233, 272
FaqI GGGAC 1 cut(s) 192
FatI CATG 2 cut(s) 150, 231
FblI GTMKAC 1 cut(s) 47
Fnu4HI GCNGC 4 cut(s) 14, 37, 40, 123
FokI GGATG 1 cut(s) 194
Fsp4HI GCNGC 4 cut(s) 14, 37, 40, 123
FspBI CTAG 1 cut(s) 60
GlaI GCGC 1 cut(s) 12
GluI GCNGC 4 cut(s) 14, 37, 40, 123
HaeIII GGCC 2 cut(s) 27, 149
HapII CCGG 1 cut(s) 33
HhaI GCGC 1 cut(s) 13
Hin1II CATG 2 cut(s) 154, 235
Hin6I GCGC 1 cut(s) 11
HinP1I GCGC 1 cut(s) 11
HincII GTYRAC 1 cut(s) 48
HindII GTYRAC 1 cut(s) 48
HpaII CCGG 1 cut(s) 33
Hpy166II GTNNAC 2 cut(s) 48, 218
Hpy188III TCNNGA 1 cut(s) 259
Hpy8I GTNNAC 2 cut(s) 48, 218
HpyCH4III ACNGT 1 cut(s) 77
HpyCH4V TGCA 2 cut(s) 67, 231
HpyF10VI GCNNNNNNNGC 1 cut(s) 33
Hsp92II CATG 2 cut(s) 154, 235
HspAI GCGC 1 cut(s) 11
KflI GGGWCCC 1 cut(s) 206
KroI GCCGGC 1 cut(s) 32
KroNI GCCGGC 1 cut(s) 34
Kzo9I GATC 1 cut(s) 115
LmnI GCTCC 1 cut(s) 163
LpnPI CCDG 5 cut(s) 2, 42, 46, 132, 156
Lsp1109I GCAGC 3 cut(s) 25, 48, 109
MaeI CTAG 1 cut(s) 60
MaeIII GTNAC 1 cut(s) 5
MalI GATC 1 cut(s) 117
MboI GATC 1 cut(s) 115
MflI RGATCY 1 cut(s) 115
MlsI TGGCCA 1 cut(s) 149
MluCI AATT 4 cut(s) 109, 130, 251, 262
MluNI TGGCCA 1 cut(s) 149
MnlI CCTC 3 cut(s) 84, 172, 184
Mox20I TGGCCA 1 cut(s) 149
MroNI GCCGGC 1 cut(s) 32
MscI TGGCCA 1 cut(s) 149
MseI TTAA 2 cut(s) 108, 293
Msp20I TGGCCA 1 cut(s) 149
MspA1I CMGCKG 2 cut(s) 16, 39
MspI CCGG 1 cut(s) 33
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 1 cut(s) 33
NaeI GCCGGC 1 cut(s) 34
NcoI CCATGG 1 cut(s) 150
NdeII GATC 1 cut(s) 115
NgoMIV GCCGGC 1 cut(s) 32
NlaIII CATG 2 cut(s) 154, 235
NlaIV GGNNCC 3 cut(s) 117, 207, 208
PdiI GCCGGC 1 cut(s) 34
PkrI GCNGC 4 cut(s) 15, 38, 41, 124
PpuMI RGGWCCY 1 cut(s) 206
Psp5II RGGWCCY 1 cut(s) 206
PspN4I GGNNCC 3 cut(s) 117, 207, 208
PspPI GGNCC 2 cut(s) 26, 206
PspPPI RGGWCCY 1 cut(s) 206
PsuI RGATCY 1 cut(s) 115
PvuII CAGCTG 1 cut(s) 16
RsaI GTAC 1 cut(s) 79
RsaNI GTAC 1 cut(s) 78
SalI GTCGAC 1 cut(s) 46
SaqAI TTAA 2 cut(s) 108, 293
SatI GCNGC 4 cut(s) 14, 37, 40, 123
Sau3AI GATC 1 cut(s) 115
Sau96I GGNCC 2 cut(s) 26, 206
SetI ASST 2 cut(s) 18, 168
SinI GGWCC 1 cut(s) 206
Sse9I AATT 4 cut(s) 109, 130, 251, 262
SsiI CCGC 2 cut(s) 39, 125
SspI AATATT 1 cut(s) 103
SspMI CTAG 1 cut(s) 60
StyI CCWWGG 2 cut(s) 150, 174
TaaI ACNGT 1 cut(s) 77
TaqI TCGA 2 cut(s) 47, 260
TasI AATT 4 cut(s) 109, 130, 251, 262
TauI GCSGC 1 cut(s) 42
Tru1I TTAA 2 cut(s) 108, 293
Tru9I TTAA 2 cut(s) 108, 293
TscAI CASTG 1 cut(s) 80
TseI GCWGC 3 cut(s) 13, 36, 122
TspRI CASTG 1 cut(s) 80
VpaK11BI GGWCC 1 cut(s) 206
XapI RAATTY 2 cut(s) 130, 262
XmiI GTMKAC 1 cut(s) 47
XspI CTAG 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.