Rmu_sc0009445.1_g000005

GPN-loop GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009445.1
OG0019764
Physical Location & Seq
Forward (+)
28521 .. 31333
2813 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009445.1_g000005.1.cds

Sequence Viewer

Length: 399 bp
atgggttatgcacagctagttattggccctgcaggcagtggcaagtccacttattgctccagtttgcaccgacactgtttagataatggaaggactctacatattgttaccttagatcctgctgcagagcattttgactaccctgtggctttggatatcagggagattattaacttggatgatgtcatggaggaacttggaccgggtcccaatggtggacttatgttctgcatggatcgccggagttgtgttttccggccgggtccgaaaacaccaacctgcaccgccttctaccgccgctaccaccgagaatcggcactagagaacaccatagggaggagcgcactgaggagtcgagctaatctggaaagtctcgtcgccggagatggccggagctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ATP_bind_1

Protein Analysis

132

Amino Acids

14.51

Weight (kDa)

6.95

Isoelectric Point (pI)

60.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019764)

Species Orthologous Gene IDs
malus_domestica MD09G1158900.v1.1
rosa_multiflora Rmu_sc0004191.1_g000034 Rmu_sc0009428.1_g000013 Rmu_sc0009445.1_g000005 Rmu_sc0029652.1_g000001
rosa_samantha Rh2BG490300 Rh2CG463900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 287
AciI CCGC 3 cut(s) 285, 295, 298
AclWI GGATC 2 cut(s) 110, 243
AcoI YGGCCR 2 cut(s) 257, 388
AfiI CCNNNNNNNGG 3 cut(s) 215, 313, 336
AluBI AGCT 3 cut(s) 16, 359, 396
AluI AGCT 3 cut(s) 16, 359, 396
Alw26I GTCTC 1 cut(s) 377
AlwI GGATC 2 cut(s) 110, 243
AoxI GGCC 3 cut(s) 25, 257, 388
ApeKI GCWGC 1 cut(s) 122
AspLEI GCGC 1 cut(s) 344
AspS9I GGNCC 4 cut(s) 26, 200, 206, 263
AsuC2I CCSGG 2 cut(s) 204, 261
AvaII GGWCC 3 cut(s) 200, 206, 263
BbvI GCAGC 1 cut(s) 109
BccI CCATC 1 cut(s) 380
BcnI CCSGG 2 cut(s) 204, 261
BcoDI GTCTC 1 cut(s) 377
BfaI CTAG 3 cut(s) 17, 320, 397
BfmI CTRYAG 2 cut(s) 30, 123
BfuAI ACCTGC 1 cut(s) 287
BglI GCCNNNNNGGC 1 cut(s) 33
BisI GCNGC 2 cut(s) 123, 298
BlsI GCNGC 2 cut(s) 124, 299
Bme1390I CCNGG 2 cut(s) 204, 261
Bme18I GGWCC 3 cut(s) 200, 206, 263
BmgT120I GGNCC 4 cut(s) 26, 200, 206, 263
BmiI GGNNCC 3 cut(s) 207, 208, 264
BmrFI CCNGG 2 cut(s) 204, 261
BpmI CTGGAG 1 cut(s) 43
BpuMI CCSGG 2 cut(s) 204, 261
Bsc4I CCNNNNNNNGG 3 cut(s) 215, 313, 336
Bse1I ACTGG 1 cut(s) 60
BseGI GGATG 1 cut(s) 184
BseLI CCNNNNNNNGG 3 cut(s) 215, 313, 336
BseMII CTCAG 1 cut(s) 338
BseNI ACTGG 1 cut(s) 60
BseRI GAGGAG 2 cut(s) 352, 364
BseX3I CGGCCG 1 cut(s) 257
BseXI GCAGC 1 cut(s) 109
BsgI GTGCAG 1 cut(s) 265
Bsh1285I CGRYCG 1 cut(s) 260
BshFI GGCC 3 cut(s) 27, 259, 390
BsiEI CGRYCG 1 cut(s) 260
BsiSI CCGG 6 cut(s) 203, 241, 256, 260, 381, 391
BslFI GGGAC 1 cut(s) 192
BslI CCNNNNNNNGG 3 cut(s) 215, 313, 336
BsmAI GTCTC 1 cut(s) 377
BsmFI GGGAC 1 cut(s) 192
BsnI GGCC 3 cut(s) 27, 259, 390
Bsp143I GATC 2 cut(s) 115, 235
BspACI CCGC 3 cut(s) 285, 295, 298
BspANI GGCC 3 cut(s) 27, 259, 390
BspCNI CTCAG 1 cut(s) 339
BspLI GGNNCC 3 cut(s) 207, 208, 264
BspMAI CTGCAG 2 cut(s) 34, 127
BspMI ACCTGC 1 cut(s) 287
BspPI GGATC 2 cut(s) 110, 243
BsrI ACTGG 1 cut(s) 60
BssMI GATC 2 cut(s) 115, 235
Bst4CI ACNGT 1 cut(s) 77
BstC8I GCNNGC 1 cut(s) 34
BstDEI CTNAG 2 cut(s) 112, 347
BstF5I GGATG 1 cut(s) 184
BstHHI GCGC 1 cut(s) 344
BstKTI GATC 2 cut(s) 118, 238
BstMAI GTCTC 1 cut(s) 377
BstMBI GATC 2 cut(s) 115, 235
BstMCI CGRYCG 1 cut(s) 260
BstMWI GCNNNNNNNGC 2 cut(s) 33, 237
BstSCI CCNGG 2 cut(s) 202, 259
BstSFI CTRYAG 2 cut(s) 30, 123
BstV1I GCAGC 1 cut(s) 109
BstX2I RGATCY 1 cut(s) 115
BstYI RGATCY 1 cut(s) 115
BstZI CGGCCG 1 cut(s) 257
BsuRI GGCC 3 cut(s) 27, 259, 390
BtsCI GGATG 1 cut(s) 184
BtsI GCAGTG 1 cut(s) 43
BtsIMutI CAGTG 3 cut(s) 43, 73, 344
BveI ACCTGC 1 cut(s) 287
Cac8I GCNNGC 1 cut(s) 34
CfoI GCGC 1 cut(s) 344
Cfr13I GGNCC 4 cut(s) 26, 200, 206, 263
CviAII CATG 2 cut(s) 187, 232
CviJI RGCY 7 cut(s) 16, 27, 149, 259, 359, 390, 396
CviKI_1 RGCY 7 cut(s) 16, 27, 149, 259, 359, 390, 396
DdeI CTNAG 2 cut(s) 112, 347
DpnI GATC 2 cut(s) 117, 237
DpnII GATC 2 cut(s) 115, 235
EaeI YGGCCR 2 cut(s) 257, 388
EagI CGGCCG 1 cut(s) 257
EclXI CGGCCG 1 cut(s) 257
Eco32I GATATC 1 cut(s) 157
Eco47I GGWCC 3 cut(s) 200, 206, 263
Eco52I CGGCCG 1 cut(s) 257
EcoO109I RGGNCCY 1 cut(s) 206
EcoRV GATATC 1 cut(s) 157
FaeI CATG 2 cut(s) 190, 235
FaiI YATR 6 cut(s) 9, 102, 188, 224, 233, 332
FaqI GGGAC 1 cut(s) 192
FatI CATG 2 cut(s) 186, 231
Fnu4HI GCNGC 2 cut(s) 123, 298
FokI GGATG 1 cut(s) 191
Fsp4HI GCNGC 2 cut(s) 123, 298
FspBI CTAG 3 cut(s) 17, 320, 397
GlaI GCGC 1 cut(s) 343
GluI GCNGC 2 cut(s) 123, 298
GsuI CTGGAG 1 cut(s) 43
HaeIII GGCC 3 cut(s) 27, 259, 390
HapII CCGG 6 cut(s) 203, 241, 256, 260, 381, 391
HhaI GCGC 1 cut(s) 344
Hin1II CATG 2 cut(s) 190, 235
Hin6I GCGC 1 cut(s) 342
HinP1I GCGC 1 cut(s) 342
HinfI GANTC 3 cut(s) 94, 311, 352
HpaII CCGG 6 cut(s) 203, 241, 256, 260, 381, 391
Hpy166II GTNNAC 2 cut(s) 48, 218
Hpy188I TCNGA 1 cut(s) 267
Hpy188III TCNNGA 1 cut(s) 365
Hpy8I GTNNAC 2 cut(s) 48, 218
Hpy99I CGWCG 1 cut(s) 380
HpyAV CCTTC 2 cut(s) 84, 298
HpyCH4III ACNGT 1 cut(s) 77
HpyCH4V TGCA 6 cut(s) 11, 32, 67, 125, 231, 282
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 237
HpyF3I CTNAG 2 cut(s) 112, 347
Hsp92II CATG 2 cut(s) 190, 235
HspAI GCGC 1 cut(s) 342
KflI GGGWCCC 1 cut(s) 206
Kzo9I GATC 2 cut(s) 115, 235
LmnI GCTCC 3 cut(s) 62, 339, 393
Lsp1109I GCAGC 1 cut(s) 109
MaeI CTAG 3 cut(s) 17, 320, 397
MaeIII GTNAC 1 cut(s) 106
MalI GATC 2 cut(s) 117, 237
MboI GATC 2 cut(s) 115, 235
MflI RGATCY 1 cut(s) 115
MlyI GAGTC 2 cut(s) 88, 361
MnlI CCTC 3 cut(s) 184, 330, 342
MseI TTAA 1 cut(s) 171
MspI CCGG 6 cut(s) 203, 241, 256, 260, 381, 391
MspR9I CCNGG 2 cut(s) 204, 261
MwoI GCNNNNNNNGC 2 cut(s) 33, 237
NciI CCSGG 2 cut(s) 204, 261
NdeII GATC 2 cut(s) 115, 235
NlaIII CATG 2 cut(s) 190, 235
NlaIV GGNNCC 3 cut(s) 207, 208, 264
PfeI GAWTC 1 cut(s) 311
PflFI GACNNNGTC 1 cut(s) 204
PkrI GCNGC 2 cut(s) 124, 299
PleI GAGTC 2 cut(s) 88, 360
PpsI GAGTC 2 cut(s) 88, 360
PpuMI RGGWCCY 1 cut(s) 206
Psp5II RGGWCCY 1 cut(s) 206
PspN4I GGNNCC 3 cut(s) 207, 208, 264
PspPI GGNCC 4 cut(s) 26, 200, 206, 263
PspPPI RGGWCCY 1 cut(s) 206
PstI CTGCAG 2 cut(s) 34, 127
PsuI RGATCY 1 cut(s) 115
PsyI GACNNNGTC 1 cut(s) 204
SaqAI TTAA 1 cut(s) 171
SatI GCNGC 2 cut(s) 123, 298
Sau3AI GATC 2 cut(s) 115, 235
Sau96I GGNCC 4 cut(s) 26, 200, 206, 263
SbfI CCTGCAGG 1 cut(s) 34
SchI GAGTC 2 cut(s) 88, 361
ScrFI CCNGG 2 cut(s) 204, 261
SdaI CCTGCAGG 1 cut(s) 34
SetI ASST 5 cut(s) 18, 113, 281, 361, 398
SfcI CTRYAG 2 cut(s) 30, 123
SinI GGWCC 3 cut(s) 200, 206, 263
Sse8387I CCTGCAGG 1 cut(s) 34
SsiI CCGC 3 cut(s) 285, 295, 298
SspMI CTAG 3 cut(s) 17, 320, 397
StyD4I CCNGG 2 cut(s) 202, 259
TaaI ACNGT 1 cut(s) 77
TaqI TCGA 1 cut(s) 355
TauI GCSGC 1 cut(s) 300
TfiI GAWTC 1 cut(s) 311
Tru1I TTAA 1 cut(s) 171
Tru9I TTAA 1 cut(s) 171
TscAI CASTG 3 cut(s) 43, 80, 351
TseI GCWGC 1 cut(s) 122
TspRI CASTG 3 cut(s) 43, 80, 351
Tth111I GACNNNGTC 1 cut(s) 204
VpaK11BI GGWCC 3 cut(s) 200, 206, 263
XspI CTAG 3 cut(s) 17, 320, 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.