Rh2CG463900

GPN-loop GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0019764
Physical Location & Seq
Reverse (-)
62713807 .. 62717993
4187 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG463900.1

Sequence Viewer

Length: 228 bp
ATGGGTTATGCACAGCTAGTTATTGGCCCTGCAGGCAGTGGCAAGTCCACTAATTGCTCCAGTTTGCACCGACACTGTTTAGATAATGGAAGGACTCTACATATTGTTAACTTAGATCCTGCTGCAGAGCATTTTGACAATTTGACTACCCTGTGGCTTTGGTACGGAGAAGATGCTGATGTGAAGGTTAAGGATTTTGATCCAGAAGATCCAGAAAACGACAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ATP_bind_1

Protein Analysis

75

Amino Acids

8.3

Weight (kDa)

4.53

Isoelectric Point (pI)

38.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP_bind_1 PF03029 7 - 45 1.1e-12 Conserved hypothetical ATP binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019764)

Species Orthologous Gene IDs
malus_domestica MD09G1158900.v1.1
rosa_multiflora Rmu_sc0004191.1_g000034 Rmu_sc0009428.1_g000013 Rmu_sc0009445.1_g000005 Rmu_sc0029652.1_g000001
rosa_samantha Rh2BG490300 Rh2CG463900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 110, 194, 203
AfaI GTAC 1 cut(s) 164
AluBI AGCT 2 cut(s) 16, 225
AluI AGCT 2 cut(s) 16, 225
AlwI GGATC 3 cut(s) 110, 194, 203
AoxI GGCC 1 cut(s) 25
ApeKI GCWGC 1 cut(s) 122
AspS9I GGNCC 1 cut(s) 26
BbvI GCAGC 1 cut(s) 109
BfaI CTAG 1 cut(s) 17
BfmI CTRYAG 2 cut(s) 30, 123
BglI GCCNNNNNGGC 1 cut(s) 33
BisI GCNGC 1 cut(s) 123
BlsI GCNGC 1 cut(s) 124
BmgT120I GGNCC 1 cut(s) 26
BmsI GCATC 1 cut(s) 163
BpmI CTGGAG 1 cut(s) 43
BsaBI GATNNNNATC 1 cut(s) 198
Bse1I ACTGG 1 cut(s) 60
Bse8I GATNNNNATC 1 cut(s) 198
BseJI GATNNNNATC 1 cut(s) 198
BseNI ACTGG 1 cut(s) 60
BseXI GCAGC 1 cut(s) 109
BshFI GGCC 1 cut(s) 27
BsnI GGCC 1 cut(s) 27
Bsp143I GATC 3 cut(s) 115, 199, 208
BspANI GGCC 1 cut(s) 27
BspMAI CTGCAG 2 cut(s) 34, 127
BspPI GGATC 3 cut(s) 110, 194, 203
BsrI ACTGG 1 cut(s) 60
BssMI GATC 3 cut(s) 115, 199, 208
Bst4CI ACNGT 1 cut(s) 77
BstC8I GCNNGC 1 cut(s) 34
BstDEI CTNAG 1 cut(s) 112
BstKTI GATC 3 cut(s) 118, 202, 211
BstMBI GATC 3 cut(s) 115, 199, 208
BstMWI GCNNNNNNNGC 1 cut(s) 33
BstSFI CTRYAG 2 cut(s) 30, 123
BstV1I GCAGC 1 cut(s) 109
BstX2I RGATCY 2 cut(s) 115, 208
BstYI RGATCY 2 cut(s) 115, 208
BsuRI GGCC 1 cut(s) 27
BtsI GCAGTG 1 cut(s) 43
BtsIMutI CAGTG 2 cut(s) 43, 73
Cac8I GCNNGC 1 cut(s) 34
Cfr13I GGNCC 1 cut(s) 26
Csp6I GTAC 1 cut(s) 163
CviJI RGCY 4 cut(s) 16, 27, 157, 225
CviKI_1 RGCY 4 cut(s) 16, 27, 157, 225
CviQI GTAC 1 cut(s) 163
DdeI CTNAG 1 cut(s) 112
DpnI GATC 3 cut(s) 117, 201, 210
DpnII GATC 3 cut(s) 115, 199, 208
FaiI YATR 2 cut(s) 9, 102
Fnu4HI GCNGC 1 cut(s) 123
Fsp4HI GCNGC 1 cut(s) 123
FspBI CTAG 1 cut(s) 17
GluI GCNGC 1 cut(s) 123
GsuI CTGGAG 1 cut(s) 43
HaeIII GGCC 1 cut(s) 27
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HinfI GANTC 1 cut(s) 94
HpaI GTTAAC 1 cut(s) 109
Hpy166II GTNNAC 2 cut(s) 48, 109
Hpy188III TCNNGA 2 cut(s) 203, 212
Hpy8I GTNNAC 2 cut(s) 48, 109
HpyAV CCTTC 2 cut(s) 84, 178
HpyCH4III ACNGT 1 cut(s) 77
HpyCH4V TGCA 4 cut(s) 11, 32, 67, 125
HpyF10VI GCNNNNNNNGC 1 cut(s) 33
HpyF3I CTNAG 1 cut(s) 112
KspAI GTTAAC 1 cut(s) 109
Kzo9I GATC 3 cut(s) 115, 199, 208
LmnI GCTCC 1 cut(s) 62
LpnPI CCDG 6 cut(s) 18, 42, 73, 132, 164, 216
Lsp1109I GCAGC 1 cut(s) 109
LweI GCATC 1 cut(s) 163
MaeI CTAG 1 cut(s) 17
MalI GATC 3 cut(s) 117, 201, 210
MboI GATC 3 cut(s) 115, 199, 208
MboII GAAGA 2 cut(s) 182, 218
MflI RGATCY 2 cut(s) 115, 208
MluCI AATT 2 cut(s) 52, 139
MlyI GAGTC 1 cut(s) 88
MseI TTAA 2 cut(s) 108, 189
MspA1I CMGCKG 1 cut(s) 225
MwoI GCNNNNNNNGC 1 cut(s) 33
NdeII GATC 3 cut(s) 115, 199, 208
PkrI GCNGC 1 cut(s) 124
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
PspPI GGNCC 1 cut(s) 26
PstI CTGCAG 2 cut(s) 34, 127
PsuI RGATCY 2 cut(s) 115, 208
PvuII CAGCTG 1 cut(s) 225
RsaI GTAC 1 cut(s) 164
RsaNI GTAC 1 cut(s) 163
SaqAI TTAA 2 cut(s) 108, 189
SatI GCNGC 1 cut(s) 123
Sau3AI GATC 3 cut(s) 115, 199, 208
Sau96I GGNCC 1 cut(s) 26
SbfI CCTGCAGG 1 cut(s) 34
SchI GAGTC 1 cut(s) 88
SdaI CCTGCAGG 1 cut(s) 34
SetI ASST 3 cut(s) 18, 189, 227
SfaNI GCATC 1 cut(s) 163
SfcI CTRYAG 2 cut(s) 30, 123
SgeI CNNG 9 cut(s) 29, 41, 45, 55, 72, 131, 163, 215, 224
Sse8387I CCTGCAGG 1 cut(s) 34
Sse9I AATT 2 cut(s) 52, 139
SspMI CTAG 1 cut(s) 17
TaaI ACNGT 1 cut(s) 77
TasI AATT 2 cut(s) 52, 139
Tru1I TTAA 2 cut(s) 108, 189
Tru9I TTAA 2 cut(s) 108, 189
TscAI CASTG 2 cut(s) 43, 80
TseI GCWGC 1 cut(s) 122
TspGWI ACGGA 1 cut(s) 180
TspRI CASTG 2 cut(s) 43, 80
XspI CTAG 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.