Rh2BG490300

GPN-loop GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0019764
Physical Location & Seq
Reverse (-)
68986033 .. 68988222
2190 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG490300.1

Sequence Viewer

Length: 306 bp
ATGGGTTATGCACAGCTAGTTATTGGCCCTGCAGGCAGTGGCAAGTCAACTTATTGCTCCAGTTTGCACCGACACTGTTTAGATAATGGAAGGACTCTACATATTGTTAACTTAGACCCTGCTGCAGAGCATTTTGACTACCCTGTGGCTTTGGATATCAGGGAGATTATTAACTTGGATGATGTTATGGAGGAACTTGGACTGGGTCCCAATGGTGGACTTATGTACCTGCATGAATACGGAGAAGATGCTGATGTGAAGGTTAAGGATTTTGATCCAGAAGATCCAGAAAACGTCGACAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ATP_bind_1

Protein Analysis

101

Amino Acids

11.1

Weight (kDa)

4.3

Isoelectric Point (pI)

36.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP_bind_1 PF03029 7 - 83 2.5e-29 Conserved hypothetical ATP binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019764)

Species Orthologous Gene IDs
malus_domestica MD09G1158900.v1.1
rosa_multiflora Rmu_sc0004191.1_g000034 Rmu_sc0009428.1_g000013 Rmu_sc0009445.1_g000005 Rmu_sc0029652.1_g000001
rosa_samantha Rh2BG490300 Rh2CG463900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 237
AccI GTMKAC 1 cut(s) 297
AclWI GGATC 2 cut(s) 269, 278
AfaI GTAC 1 cut(s) 227
AfiI CCNNNNNNNGG 1 cut(s) 215
AluBI AGCT 2 cut(s) 16, 303
AluI AGCT 2 cut(s) 16, 303
AlwI GGATC 2 cut(s) 269, 278
AoxI GGCC 1 cut(s) 25
ApeKI GCWGC 1 cut(s) 122
AspS9I GGNCC 2 cut(s) 26, 206
AvaII GGWCC 1 cut(s) 206
BaeI ACNNNNGTAYC 2 cut(s) 209, 242
BbvI GCAGC 1 cut(s) 109
BfaI CTAG 1 cut(s) 17
BfmI CTRYAG 2 cut(s) 30, 123
BfuAI ACCTGC 1 cut(s) 237
BglI GCCNNNNNGGC 1 cut(s) 33
BisI GCNGC 1 cut(s) 123
BlsI GCNGC 1 cut(s) 124
Bme18I GGWCC 1 cut(s) 206
BmgT120I GGNCC 2 cut(s) 26, 206
BmiI GGNNCC 2 cut(s) 207, 208
BmrI ACTGGG 1 cut(s) 212
BmsI GCATC 1 cut(s) 238
BmuI ACTGGG 1 cut(s) 212
BpmI CTGGAG 1 cut(s) 43
BsaBI GATNNNNATC 1 cut(s) 273
Bsc4I CCNNNNNNNGG 1 cut(s) 215
Bse1I ACTGG 2 cut(s) 60, 207
Bse8I GATNNNNATC 1 cut(s) 273
BseGI GGATG 1 cut(s) 184
BseJI GATNNNNATC 1 cut(s) 273
BseLI CCNNNNNNNGG 1 cut(s) 215
BseNI ACTGG 2 cut(s) 60, 207
BseXI GCAGC 1 cut(s) 109
BshFI GGCC 1 cut(s) 27
BslFI GGGAC 1 cut(s) 192
BslI CCNNNNNNNGG 1 cut(s) 215
BsmFI GGGAC 1 cut(s) 192
BsnI GGCC 1 cut(s) 27
Bsp143I GATC 2 cut(s) 274, 283
BspANI GGCC 1 cut(s) 27
BspLI GGNNCC 2 cut(s) 207, 208
BspMAI CTGCAG 2 cut(s) 34, 127
BspMI ACCTGC 1 cut(s) 237
BspPI GGATC 2 cut(s) 269, 278
BsrI ACTGG 2 cut(s) 60, 207
BssMI GATC 2 cut(s) 274, 283
Bst4CI ACNGT 1 cut(s) 77
BstC8I GCNNGC 1 cut(s) 34
BstDEI CTNAG 1 cut(s) 112
BstF5I GGATG 1 cut(s) 184
BstKTI GATC 2 cut(s) 277, 286
BstMBI GATC 2 cut(s) 274, 283
BstMWI GCNNNNNNNGC 1 cut(s) 33
BstSFI CTRYAG 2 cut(s) 30, 123
BstV1I GCAGC 1 cut(s) 109
BstX2I RGATCY 1 cut(s) 283
BstYI RGATCY 1 cut(s) 283
BsuRI GGCC 1 cut(s) 27
BtsCI GGATG 1 cut(s) 184
BtsI GCAGTG 1 cut(s) 43
BtsIMutI CAGTG 2 cut(s) 43, 73
BveI ACCTGC 1 cut(s) 237
Cac8I GCNNGC 1 cut(s) 34
Cfr13I GGNCC 2 cut(s) 26, 206
Csp6I GTAC 1 cut(s) 226
CviAII CATG 1 cut(s) 233
CviJI RGCY 4 cut(s) 16, 27, 149, 303
CviKI_1 RGCY 4 cut(s) 16, 27, 149, 303
CviQI GTAC 1 cut(s) 226
DdeI CTNAG 1 cut(s) 112
DpnI GATC 2 cut(s) 276, 285
DpnII GATC 2 cut(s) 274, 283
Eco32I GATATC 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 206
EcoO109I RGGNCCY 1 cut(s) 206
EcoRV GATATC 1 cut(s) 157
FaeI CATG 1 cut(s) 236
FaiI YATR 5 cut(s) 9, 102, 188, 224, 234
FaqI GGGAC 1 cut(s) 192
FatI CATG 1 cut(s) 232
FblI GTMKAC 1 cut(s) 297
Fnu4HI GCNGC 1 cut(s) 123
FokI GGATG 1 cut(s) 191
Fsp4HI GCNGC 1 cut(s) 123
FspBI CTAG 1 cut(s) 17
GluI GCNGC 1 cut(s) 123
GsuI CTGGAG 1 cut(s) 43
HaeIII GGCC 1 cut(s) 27
Hin1II CATG 1 cut(s) 236
HincII GTYRAC 3 cut(s) 48, 109, 298
HindII GTYRAC 3 cut(s) 48, 109, 298
HinfI GANTC 1 cut(s) 94
HpaI GTTAAC 1 cut(s) 109
Hpy166II GTNNAC 4 cut(s) 48, 109, 218, 298
Hpy188III TCNNGA 2 cut(s) 278, 287
Hpy8I GTNNAC 4 cut(s) 48, 109, 218, 298
Hpy99I CGWCG 1 cut(s) 299
HpyAV CCTTC 2 cut(s) 84, 253
HpyCH4III ACNGT 1 cut(s) 77
HpyCH4IV ACGT 1 cut(s) 294
HpyCH4V TGCA 5 cut(s) 11, 32, 67, 125, 232
HpyF10VI GCNNNNNNNGC 1 cut(s) 33
HpyF3I CTNAG 1 cut(s) 112
HpySE526I ACGT 1 cut(s) 294
Hsp92II CATG 1 cut(s) 236
KflI GGGWCCC 1 cut(s) 206
KspAI GTTAAC 1 cut(s) 109
Kzo9I GATC 2 cut(s) 274, 283
LmnI GCTCC 1 cut(s) 62
Lsp1109I GCAGC 1 cut(s) 109
LweI GCATC 1 cut(s) 238
MaeI CTAG 1 cut(s) 17
MaeII ACGT 1 cut(s) 294
MalI GATC 2 cut(s) 276, 285
MboI GATC 2 cut(s) 274, 283
MboII GAAGA 2 cut(s) 257, 293
MflI RGATCY 1 cut(s) 283
MlyI GAGTC 1 cut(s) 88
MnlI CCTC 1 cut(s) 184
MseI TTAA 3 cut(s) 108, 171, 264
MspA1I CMGCKG 1 cut(s) 303
MwoI GCNNNNNNNGC 1 cut(s) 33
NdeII GATC 2 cut(s) 274, 283
NlaIII CATG 1 cut(s) 236
NlaIV GGNNCC 2 cut(s) 207, 208
PflFI GACNNNGTC 1 cut(s) 204
PkrI GCNGC 1 cut(s) 124
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
PpuMI RGGWCCY 1 cut(s) 206
Psp5II RGGWCCY 1 cut(s) 206
PspN4I GGNNCC 2 cut(s) 207, 208
PspPI GGNCC 2 cut(s) 26, 206
PspPPI RGGWCCY 1 cut(s) 206
PstI CTGCAG 2 cut(s) 34, 127
PsuI RGATCY 1 cut(s) 283
PsyI GACNNNGTC 1 cut(s) 204
PvuII CAGCTG 1 cut(s) 303
RsaI GTAC 1 cut(s) 227
RsaNI GTAC 1 cut(s) 226
SalI GTCGAC 1 cut(s) 296
SaqAI TTAA 3 cut(s) 108, 171, 264
SatI GCNGC 1 cut(s) 123
Sau3AI GATC 2 cut(s) 274, 283
Sau96I GGNCC 2 cut(s) 26, 206
SbfI CCTGCAGG 1 cut(s) 34
SchI GAGTC 1 cut(s) 88
SdaI CCTGCAGG 1 cut(s) 34
SetI ASST 5 cut(s) 18, 231, 264, 297, 305
SfaNI GCATC 1 cut(s) 238
SfcI CTRYAG 2 cut(s) 30, 123
SinI GGWCC 1 cut(s) 206
Sse8387I CCTGCAGG 1 cut(s) 34
SspMI CTAG 1 cut(s) 17
TaaI ACNGT 1 cut(s) 77
TaiI ACGT 1 cut(s) 297
TaqI TCGA 1 cut(s) 297
Tru1I TTAA 3 cut(s) 108, 171, 264
Tru9I TTAA 3 cut(s) 108, 171, 264
TscAI CASTG 2 cut(s) 43, 80
TseI GCWGC 1 cut(s) 122
TspDTI ATGAA 1 cut(s) 249
TspGWI ACGGA 1 cut(s) 255
TspRI CASTG 2 cut(s) 43, 80
Tth111I GACNNNGTC 1 cut(s) 204
VpaK11BI GGWCC 1 cut(s) 206
XmiI GTMKAC 1 cut(s) 297
XspI CTAG 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.