MD09G1165300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0020158
Physical Location & Seq
Forward (+)
13532755 .. 13533576
822 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1165300.v1.1.491

Sequence Viewer

Length: 822 bp
ATGACTACTCTAAGCACTCTCTTCCGCCTCCACACTCCGCCGTCAAACCCACCACTCTCCCACACCTCTCCGCACCCCCTCCGCCTCTCCTGCTCCTTCAAACCCACAAAACCCCCCAAACCACCTTCTCTATCAGCCTCAACACCGCCGCCGCCGCCCGCCAGAGACAGAGTCATAGACTTCGGAAAACACAAGGGCAAGATGCTGGGCACCCTCCCTTCTGCCTACCTCAAGTGGGTCTCCAAAAATCTCCGCGCTCGCGACTTCGAGGACTGGGCCAAGCTCGCCGACCAGGTCCTCGACGACGCCATTTACCGGGACCGGATCGAGTGGGAGCTGGCGGAGAACGTCCTGAACGGAAATCGGAGGAATTCTATCGCCGCGGGTGGAGTTTCGGAGCTGTTGGAGATCAGCGAGAGGTTCGGTTGGGATAATGAGGACAAAATTGGGTGGAGCAGGGTGAACTTTGAGCTTTTGGGGACGTCGAAAGGGGGGAGAATTCCGAGGAGGAGTGAGAAGGAGGGAGGAGAGAGAAGGGAGGAGAGGGGGAGGGGGAGGGAAGAGGAGAAGGAGGTAGTTGAGGATTTGGGGGAGGTCGGAGAGAGGCGGAGGGAGAGGAGAGTGAGGGTGAGGCAGAGAATGAGGAGGGAGGAGAAGTTGGGGATTTTTGAAAAGAGTGGGGGCAGTGTTGGAAATGGAGTTGGGTTAGGGAGGGAGAAAGAGAGTGGCGGTGAGGATTGTATGGTGGAGAATTCGAAACGGTTTCCTGGACGTGAAGGTCTGTTGAAAATGGCATACAGTAGAAGAATGGGGAGATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QSregVF_b

Protein Analysis

274

Amino Acids

31.0

Weight (kDa)

9.87

Isoelectric Point (pI)

51.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020158)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51080
fragaria_vesca FvH4_6g36940
malus_domestica MD09G1165300.v1.1
prunus_persica Prupe.3G023000_v2.0.a1
pyrus_communis pycom04g06160
rosa_wichuraiana Rw2G038390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 777
AatII GACGTC 1 cut(s) 485
AccB1I GGYRCC 1 cut(s) 209
AccII CGCG 3 cut(s) 255, 261, 383
AclWI GGATC 1 cut(s) 332
AcsI RAATTY 3 cut(s) 370, 498, 751
AcyI GRCGYC 2 cut(s) 306, 482
AfiI CCNNNNNNNGG 2 cut(s) 235, 315
AgsI TTSAA 3 cut(s) 100, 671, 787
AjiI CACGTC 1 cut(s) 773
AjnI CCWGG 2 cut(s) 291, 766
AluBI AGCT 4 cut(s) 283, 337, 400, 472
AluI AGCT 4 cut(s) 283, 337, 400, 472
Alw26I GTCTC 2 cut(s) 159, 244
AlwI GGATC 1 cut(s) 332
AoxI GGCC 1 cut(s) 276
ApoI RAATTY 3 cut(s) 370, 498, 751
AspLEI GCGC 1 cut(s) 257
AspS9I GGNCC 3 cut(s) 276, 295, 319
AsuC2I CCSGG 1 cut(s) 317
AsuHPI GGTGA 3 cut(s) 472, 640, 743
AsuII TTCGAA 1 cut(s) 755
AvaII GGWCC 2 cut(s) 295, 319
BaeGI GKGCMC 1 cut(s) 212
BanI GGYRCC 1 cut(s) 209
BceAI ACGGC 1 cut(s) 25
BciT130I CCWGG 2 cut(s) 293, 768
BcnI CCSGG 1 cut(s) 317
BcoDI GTCTC 2 cut(s) 159, 244
BisI GCNGC 4 cut(s) 149, 152, 155, 381
BlsI GCNGC 4 cut(s) 150, 153, 156, 382
Bme1390I CCNGG 3 cut(s) 293, 317, 768
Bme18I GGWCC 2 cut(s) 295, 319
BmgBI CACGTC 1 cut(s) 773
BmgT120I GGNCC 3 cut(s) 276, 295, 319
BmiI GGNNCC 2 cut(s) 211, 320
BmrFI CCNGG 3 cut(s) 293, 317, 768
BmrI ACTGGG 1 cut(s) 283
BmsI GCATC 1 cut(s) 192
BmuI ACTGGG 1 cut(s) 283
Bpu14I TTCGAA 1 cut(s) 755
BpuEI CTTGAG 1 cut(s) 215
BpuMI CCSGG 1 cut(s) 317
BsaHI GRCGYC 2 cut(s) 306, 482
BsaI GGTCTC 1 cut(s) 244
BsaJI CCNNGG 2 cut(s) 381, 503
BsaWI WCCGGW 1 cut(s) 321
BsaXI ACNNNNNCTCC 2 cut(s) 641, 671
Bsc4I CCNNNNNNNGG 2 cut(s) 235, 315
Bse1I ACTGG 1 cut(s) 278
BseBI CCWGG 2 cut(s) 293, 768
BseDI CCNNGG 2 cut(s) 381, 503
BseLI CCNNNNNNNGG 2 cut(s) 235, 315
BseNI ACTGG 1 cut(s) 278
BseRI GAGGAG 8 cut(s) 520, 523, 540, 554, 578, 631, 658, 665
BseSI GKGCMC 1 cut(s) 212
BseYI CCCAGC 1 cut(s) 205
Bsh1236I CGCG 3 cut(s) 255, 261, 383
BshFI GGCC 1 cut(s) 278
BshNI GGYRCC 1 cut(s) 209
BsiSI CCGG 2 cut(s) 316, 322
BslFI GGGAC 2 cut(s) 332, 493
BslI CCNNNNNNNGG 2 cut(s) 235, 315
BsmAI GTCTC 2 cut(s) 159, 244
BsmFI GGGAC 2 cut(s) 332, 493
BsnI GGCC 1 cut(s) 278
Bso31I GGTCTC 1 cut(s) 244
Bsp119I TTCGAA 1 cut(s) 755
Bsp1286I GDGCHC 1 cut(s) 212
Bsp143I GATC 2 cut(s) 324, 408
Bsp68I TCGCGA 1 cut(s) 261
BspANI GGCC 1 cut(s) 278
BspFNI CGCG 3 cut(s) 255, 261, 383
BspLI GGNNCC 2 cut(s) 211, 320
BspPI GGATC 1 cut(s) 332
BspT104I TTCGAA 1 cut(s) 755
BspT107I GGYRCC 1 cut(s) 209
BspTNI GGTCTC 1 cut(s) 244
BsrI ACTGG 1 cut(s) 278
BssECI CCNNGG 2 cut(s) 381, 503
BssMI GATC 2 cut(s) 324, 408
BssNI GRCGYC 2 cut(s) 306, 482
Bst2UI CCWGG 2 cut(s) 293, 768
Bst4CI ACNGT 2 cut(s) 762, 800
Bst6I CTCTTC 2 cut(s) 26, 555
BstACI GRCGYC 2 cut(s) 306, 482
BstBI TTCGAA 1 cut(s) 755
BstC8I GCNNGC 4 cut(s) 159, 259, 285, 339
BstDEI CTNAG 1 cut(s) 11
BstDSI CCRYGG 1 cut(s) 381
BstFNI CGCG 3 cut(s) 255, 261, 383
BstHHI GCGC 1 cut(s) 257
BstKTI GATC 2 cut(s) 327, 411
BstMAI GTCTC 2 cut(s) 159, 244
BstMBI GATC 2 cut(s) 324, 408
BstMWI GCNNNNNNNGC 3 cut(s) 90, 154, 284
BstNI CCWGG 2 cut(s) 293, 768
BstSCI CCNGG 3 cut(s) 291, 315, 766
BstSLI GKGCMC 1 cut(s) 212
BstUI CGCG 3 cut(s) 255, 261, 383
BsuRI GGCC 1 cut(s) 278
BtgI CCRYGG 1 cut(s) 381
BtrI CACGTC 1 cut(s) 773
BtsI GCAGTG 1 cut(s) 691
BtsIMutI CAGTG 1 cut(s) 691
BtuMI TCGCGA 1 cut(s) 261
Cac8I GCNNGC 4 cut(s) 159, 259, 285, 339
CfoI GCGC 1 cut(s) 257
Cfr13I GGNCC 3 cut(s) 276, 295, 319
Cfr42I CCGCGG 1 cut(s) 384
CseI GACGC 1 cut(s) 314
CsiI ACCWGGT 1 cut(s) 291
CviJI RGCY 6 cut(s) 137, 278, 283, 337, 400, 472
CviKI_1 RGCY 6 cut(s) 137, 278, 283, 337, 400, 472
DdeI CTNAG 1 cut(s) 11
DpnI GATC 2 cut(s) 326, 410
DpnII GATC 2 cut(s) 324, 408
DrdI GACNNNNNNGTC 1 cut(s) 777
DseDI GACNNNNNNGTC 1 cut(s) 777
Eam1104I CTCTTC 2 cut(s) 26, 555
EarI CTCTTC 2 cut(s) 26, 555
EciI GGCGGA 5 cut(s) 14, 27, 71, 356, 622
Eco31I GGTCTC 1 cut(s) 244
Eco47I GGWCC 2 cut(s) 295, 319
EcoO109I RGGNCCY 1 cut(s) 295
EcoRI GAATTC 3 cut(s) 370, 498, 751
EcoRII CCWGG 2 cut(s) 291, 766
FaiI YATR 3 cut(s) 176, 743, 796
FaqI GGGAC 2 cut(s) 332, 493
FauI CCCGC 2 cut(s) 166, 376
Fnu4HI GCNGC 4 cut(s) 149, 152, 155, 381
Fsp4HI GCNGC 4 cut(s) 149, 152, 155, 381
GlaI GCGC 1 cut(s) 256
GluI GCNGC 4 cut(s) 149, 152, 155, 381
GsaI CCCAGC 1 cut(s) 209
HaeIII GGCC 1 cut(s) 278
HapII CCGG 2 cut(s) 316, 322
HgaI GACGC 1 cut(s) 314
HhaI GCGC 1 cut(s) 257
Hin1I GRCGYC 2 cut(s) 306, 482
Hin6I GCGC 1 cut(s) 255
HinP1I GCGC 1 cut(s) 255
HinfI GANTC 1 cut(s) 171
HpaII CCGG 2 cut(s) 316, 322
HphI GGTGA 3 cut(s) 472, 640, 743
Hpy166II GTNNAC 1 cut(s) 463
Hpy188I TCNGA 5 cut(s) 185, 366, 397, 504, 599
Hpy188III TCNNGA 2 cut(s) 260, 352
Hpy8I GTNNAC 1 cut(s) 463
Hpy99I CGWCG 3 cut(s) 305, 308, 487
HpyAV CCTTC 7 cut(s) 106, 135, 228, 511, 528, 562, 770
HpyCH4III ACNGT 2 cut(s) 762, 800
HpyCH4IV ACGT 3 cut(s) 348, 482, 772
HpyF10VI GCNNNNNNNGC 3 cut(s) 90, 154, 284
HpyF3I CTNAG 1 cut(s) 11
HpySE526I ACGT 3 cut(s) 348, 482, 772
Hsp92I GRCGYC 2 cut(s) 306, 482
HspAI GCGC 1 cut(s) 255
KspI CCGCGG 1 cut(s) 384
Kzo9I GATC 2 cut(s) 324, 408
LmnI GCTCC 4 cut(s) 98, 334, 397, 453
LweI GCATC 1 cut(s) 192
MabI ACCWGGT 1 cut(s) 291
MaeII ACGT 3 cut(s) 348, 482, 772
MalI GATC 2 cut(s) 326, 410
MboI GATC 2 cut(s) 324, 408
MboII GAAGA 3 cut(s) 13, 572, 816
MhlI GDGCHC 1 cut(s) 212
MluCI AATT 4 cut(s) 370, 444, 498, 751
MlyI GAGTC 1 cut(s) 180
MmeI TCCRAC 3 cut(s) 384, 577, 670
MspA1I CMGCKG 1 cut(s) 383
MspI CCGG 2 cut(s) 316, 322
MspR9I CCNGG 3 cut(s) 293, 317, 768
MvaI CCWGG 2 cut(s) 293, 768
MvnI CGCG 3 cut(s) 255, 261, 383
MwoI GCNNNNNNNGC 3 cut(s) 90, 154, 284
NciI CCSGG 1 cut(s) 317
NdeII GATC 2 cut(s) 324, 408
NlaIV GGNNCC 2 cut(s) 211, 320
NruI TCGCGA 1 cut(s) 261
NspV TTCGAA 1 cut(s) 755
PflFI GACNNNGTC 2 cut(s) 170, 293
PfoI TCCNGGA 1 cut(s) 766
PkrI GCNGC 4 cut(s) 150, 153, 156, 382
PleI GAGTC 1 cut(s) 179
PpsI GAGTC 1 cut(s) 179
PpuMI RGGWCCY 1 cut(s) 295
Psp5II RGGWCCY 1 cut(s) 295
Psp6I CCWGG 2 cut(s) 291, 766
PspFI CCCAGC 1 cut(s) 205
PspGI CCWGG 2 cut(s) 291, 766
PspN4I GGNNCC 2 cut(s) 211, 320
PspPI GGNCC 3 cut(s) 276, 295, 319
PspPPI RGGWCCY 1 cut(s) 295
PsyI GACNNNGTC 2 cut(s) 170, 293
RruI TCGCGA 1 cut(s) 261
SacII CCGCGG 1 cut(s) 384
SatI GCNGC 4 cut(s) 149, 152, 155, 381
Sau3AI GATC 2 cut(s) 324, 408
Sau96I GGNCC 3 cut(s) 276, 295, 319
SchI GAGTC 1 cut(s) 180
ScrFI CCNGG 3 cut(s) 293, 317, 768
SduI GDGCHC 1 cut(s) 212
SexAI ACCWGGT 1 cut(s) 291
SfaNI GCATC 1 cut(s) 192
Sfr303I CCGCGG 1 cut(s) 384
SfuI TTCGAA 1 cut(s) 755
SgrBI CCGCGG 1 cut(s) 384
SinI GGWCC 2 cut(s) 295, 319
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
Sse9I AATT 4 cut(s) 370, 444, 498, 751
StyD4I CCNGG 3 cut(s) 291, 315, 766
TaaI ACNGT 2 cut(s) 762, 800
TaiI ACGT 3 cut(s) 351, 485, 775
TaqI TCGA 5 cut(s) 267, 300, 327, 485, 755
TasI AATT 4 cut(s) 370, 444, 498, 751
TauI GCSGC 4 cut(s) 151, 154, 157, 383
TscAI CASTG 1 cut(s) 691
TspGWI ACGGA 1 cut(s) 372
TspRI CASTG 1 cut(s) 691
Tth111I GACNNNGTC 2 cut(s) 170, 293
VpaK11BI GGWCC 2 cut(s) 295, 319
XapI RAATTY 3 cut(s) 370, 498, 751
ZraI GACGTC 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.