MD09G1169900.v1.1

Complex I intermediate-associated protein 30 (CIA30)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005280
Physical Location & Seq
Reverse (-)
14184878 .. 14189280
4403 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1169900.v1.1.491

Sequence Viewer

Length: 1848 bp
ATGGCCGTCACTAAAGCCATCAGCACCACCAGCTTGAGCAATGGCAACGGCGGCGGCGGCGGTAAAAATAACATCACTCACTATTACCAATGGCGGAGGCCCATTTCTCTCCCAATCCCAAAACCCAACTCCCAAAACTTCACAATCGCCTCCCGAAACCGTTCGTTGTTAATTCCCAGGGCCGCCGGACCTTCCTCCGAACCCACCAACAATGGCGGCGGAAAAAAAACCAAAACCAAGAACAAAACTGTTGATCCTGCTTCTACTGAGGAGAAAAGTGATTCCCCAATTAATCAAATCCAAGAACAGCAGGAGCAGAAGCAGAAGCAGAAGCAGCTGGGATTGGATTTGGAGGACGTGAACCCGGTCGGGCTCGGTCGGAGGTCGCGCCAGCTGTTCGATGAAGTGTGGCGCAAGTTTTCTGGCCTCGGGCAGATCTCAAGAACCAGTCTTCCCGACGAAAGAGACGCGCTTGACGCTCTGCTCATCAGGGAAGGACCCATGTGCGAGTTCGTCATCCCCGGCGCCCAGAACACCACCGTCTTGGTCGTCGGCGCCACCTCGAGTATCGGCCGGATAGTGGTCCGCAAGCTCATGCTCCGAGGGTACACTGTTAAGGCGCTGGTGAGGAATGCTGATCAGGAAGTGGTGGAGATGTTGCCGAGATCGGTAGAGATTGTGACCGGGGATGTGGGTGATCCTGCTACCCTATACGCGGCGGTACAGGGCTGTAACAAGATTATCTATTGTGCTACTGCACGTTCTACTATCAGTGGAGACCTCTACAGAGTTGATCAAAGAGGGGTCTACAACCTCACCAAAGCATTCCAGGACTATAACAACAAAATGGCACAGTTACGAGCAGGAAAGAGTAGCAAGAGCAAACTTACTATTGTAAAGTTCAAGACTACAGAGTCGGTGGATGGATGGGAAGTTCGTCAGGGGACTTACTTTCAGGATGTGGTTGCGTCTAAGTATGATGGAGGAATGGACGCCAAGTTCGAATTCACTTTCACCGGAGATGCTGTTTTCTCCGGATATGTTTTCACCAGAGGAGGGTATGTTGAACTGTCAAAAAAGCTTTCGCTTCCTTTGGGTCGAACCCTTGACAGGTATGAAGGTCTAGTTCTTTCTGTTGGTGGGAATGGAAGATCTTACATATTAATTCTTGAAGCTGGTCCTTCGGCGGATACATCTCAAAGTAAATTATACTTTTCTAGATTCAACACAAAAGCAGGATTTTGTAGGGTCAGAGTACCGTTTTCATCATTCCGCCCTGTGAAACCAGATGATCCACCACTAGACCCATTCCTTGTACATACATTAACCATACGTTTTGAGCCCAGAAGACAGAAAGCTGTTGAAGGACGTACGGGAGTGCAGCAGCAAGACCCAAGAAGTTTTATGCTTATACTAGAATACATAAAAGCTTTGCCAACTGGGCAAGAAACGGACTTCGTTTTAGTTTCTTGTACCGGATCAGGAATAGAACCTAATAGAAGGGAGCAGGTTCTTAAAGCAAAGAGGGCTGGGGAAGAGTCATTGCGAAAATCAGGGCTTGGATACACAATCATTCGTCCTGGTCCCCTGAAGGAAGAACCAGGTGGACAACGTGCTCTCATATTCGATCAAGGAAACAGGATTTCTCAGGGGATCAGCTGTGCCGATGTAGCTGATATCTGCGTGAAGGCATTGCATGATTCGACTGCAAGGAACAAAAGCTTTGATGTATGTTATGAATACGTTGCCGATCAAGGAAAGGAACTTTATGAGCTGGTGGCGCATTTGCCTGACAAAGCCAATAACTATCTGACACCGGCACTGTCCGCTCTAGAGAAGAATACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIA30 NAD_binding_10

Protein Analysis

616

Amino Acids

67.73

Weight (kDa)

9.35

Isoelectric Point (pI)

40.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 178 - 252 2.4e-11 NmrA-like family
Epimerase PF01370 181 - 281 3.3e-06 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 185 - 278 3.9e-12 NAD(P)H-binding
CIA30 PF08547 328 - 427 1e-08 Complex I intermediate-associated protein 30 (CIA30)
NAD_binding_10 PF13460 486 - 567 9.8e-13 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 913
Acc36I ACCTGC 1 cut(s) 1498
AccB1I GGYRCC 2 cut(s) 524, 554
AccBSI CCGCTC 1 cut(s) 1829
AccI GTMKAC 1 cut(s) 807
AccII CGCG 3 cut(s) 388, 470, 716
AccIII TCCGGA 1 cut(s) 1034
AclWI GGATC 5 cut(s) 248, 692, 1286, 1486, 1661
AcoI YGGCCR 2 cut(s) 3, 571
AcsI RAATTY 1 cut(s) 1004
AcuI CTGAAG 1 cut(s) 1610
AcyI GRCGYC 3 cut(s) 525, 555, 993
AfaI GTAC 6 cut(s) 608, 723, 1257, 1317, 1372, 1474
AfiI CCNNNNNNNGG 4 cut(s) 580, 715, 1056, 1110
AgsI TTSAA 5 cut(s) 904, 1067, 1172, 1225, 1364
AjiI CACGTC 1 cut(s) 358
AjnI CCWGG 4 cut(s) 176, 828, 1579, 1600
AjuI GAANNNNNNNTTGG 2 cut(s) 989, 1021
AloI GAACNNNNNNTCC 6 cut(s) 436, 468, 918, 950, 983, 1015
Alw21I GWGCWC 1 cut(s) 1618
Alw26I GTCTC 2 cut(s) 459, 771
AlwI GGATC 5 cut(s) 248, 692, 1286, 1486, 1661
Ama87I CYCGRG 2 cut(s) 428, 562
Aor13HI TCCGGA 1 cut(s) 1034
AoxI GGCC 5 cut(s) 3, 98, 180, 424, 571
ApeKI GCWGC 3 cut(s) 334, 1381, 1384
ApoI RAATTY 1 cut(s) 1004
ArsI GACNNNNNNTTYG 2 cut(s) 983, 1015
AseI ATTAAT 2 cut(s) 291, 1163
Asp700I GAANNNNTTC 1 cut(s) 160
AspLEI GCGC 7 cut(s) 390, 414, 472, 527, 557, 622, 1783
AspS9I GGNCC 7 cut(s) 99, 180, 188, 497, 583, 1178, 1583
AsuC2I CCSGG 3 cut(s) 365, 522, 685
AsuHPI GGTGA 5 cut(s) 637, 707, 808, 1006, 1039
AsuII TTCGAA 1 cut(s) 1002
AvaI CYCGRG 2 cut(s) 428, 562
AvaII GGWCC 5 cut(s) 188, 497, 583, 1178, 1583
BanI GGYRCC 2 cut(s) 524, 554
BanII GRGCYC 2 cut(s) 375, 1344
BbsI GAAGAC 2 cut(s) 443, 1354
Bbv12I GWGCWC 1 cut(s) 1618
BbvI GCAGC 3 cut(s) 346, 1393, 1396
BccI CCATC 4 cut(s) 26, 917, 921, 974
BceAI ACGGC 1 cut(s) 64
BciT130I CCWGG 4 cut(s) 178, 830, 1581, 1602
BciVI GTATCC 2 cut(s) 1183, 1556
BclI TGATCA 2 cut(s) 637, 793
BcnI CCSGG 3 cut(s) 365, 522, 685
BcoDI GTCTC 2 cut(s) 459, 771
BfaI CTAG 5 cut(s) 1124, 1218, 1301, 1415, 1832
BfmI CTRYAG 2 cut(s) 784, 909
BfoI RGCGCY 3 cut(s) 528, 558, 623
BfuAI ACCTGC 1 cut(s) 1498
BfuI GTATCC 2 cut(s) 1183, 1556
BglI GCCNNNNNGGC 1 cut(s) 1441
BglII AGATCT 2 cut(s) 435, 1151
BisI GCNGC 9 cut(s) 52, 55, 58, 183, 217, 335, 717, 1382, 1385
BlsI GCNGC 9 cut(s) 53, 56, 59, 184, 218, 336, 718, 1383, 1386
Bme1390I CCNGG 7 cut(s) 178, 365, 522, 685, 830, 1581, 1602
Bme18I GGWCC 5 cut(s) 188, 497, 583, 1178, 1583
BmeT110I CYCGRG 2 cut(s) 428, 562
BmgBI CACGTC 1 cut(s) 358
BmgT120I GGNCC 7 cut(s) 99, 180, 188, 497, 583, 1178, 1583
BmiI GGNNCC 4 cut(s) 499, 526, 556, 1585
BmrFI CCNGG 7 cut(s) 178, 365, 522, 685, 830, 1581, 1602
BmrI ACTGGG 1 cut(s) 1449
BmsI GCATC 1 cut(s) 1012
BmuI ACTGGG 1 cut(s) 1449
BpiI GAAGAC 2 cut(s) 443, 1354
Bpu14I TTCGAA 1 cut(s) 1002
BpuEI CTTGAG 2 cut(s) 55, 424
BpuMI CCSGG 3 cut(s) 365, 522, 685
BsaHI GRCGYC 3 cut(s) 525, 555, 993
BsaI GGTCTC 1 cut(s) 771
BsaJI CCNNGG 6 cut(s) 176, 177, 427, 520, 601, 684
BsaWI WCCGGW 3 cut(s) 1016, 1034, 1475
BsaXI ACNNNNNCTCC 4 cut(s) 1011, 1041, 1047, 1077
Bsc4I CCNNNNNNNGG 4 cut(s) 580, 715, 1056, 1110
Bse118I RCCGGY 1 cut(s) 1816
Bse1I ACTGG 2 cut(s) 447, 1444
Bse3DI GCAATG 3 cut(s) 46, 1541, 1691
BseAI TCCGGA 1 cut(s) 1034
BseBI CCWGG 4 cut(s) 178, 830, 1581, 1602
BseDI CCNNGG 6 cut(s) 176, 177, 427, 520, 601, 684
BseGI GGATG 5 cut(s) 516, 694, 928, 932, 964
BseLI CCNNNNNNNGG 4 cut(s) 580, 715, 1056, 1110
BseMI GCAATG 3 cut(s) 46, 1541, 1691
BseMII CTCAG 2 cut(s) 258, 1661
BseNI ACTGG 2 cut(s) 447, 1444
BseRI GAGGAG 2 cut(s) 284, 1068
BseX3I CGGCCG 1 cut(s) 571
BseXI GCAGC 3 cut(s) 346, 1393, 1396
BseYI CCCAGC 2 cut(s) 337, 1529
BsgI GTGCAG 2 cut(s) 741, 1400
Bsh1236I CGCG 3 cut(s) 388, 470, 716
Bsh1285I CGRYCG 3 cut(s) 369, 379, 574
BshFI GGCC 5 cut(s) 5, 100, 182, 426, 573
BshNI GGYRCC 2 cut(s) 524, 554
BsiEI CGRYCG 3 cut(s) 369, 379, 574
BsiHKAI GWGCWC 1 cut(s) 1618
BsiHKCI CYCGRG 2 cut(s) 428, 562
BsiSI CCGG 9 cut(s) 186, 365, 522, 574, 684, 1017, 1035, 1476, 1817
BsiWI CGTACG 1 cut(s) 1370
BslFI GGGAC 2 cut(s) 958, 1569
BslI CCNNNNNNNGG 4 cut(s) 580, 715, 1056, 1110
BsmAI GTCTC 2 cut(s) 459, 771
BsmBI CGTCTC 1 cut(s) 459
BsmFI GGGAC 2 cut(s) 958, 1569
BsmI GAATGC 2 cut(s) 637, 824
BsnI GGCC 5 cut(s) 5, 100, 182, 426, 573
Bso31I GGTCTC 1 cut(s) 771
BsoBI CYCGRG 2 cut(s) 428, 562
Bsp119I TTCGAA 1 cut(s) 1002
Bsp1286I GDGCHC 3 cut(s) 375, 1344, 1618
Bsp13I TCCGGA 1 cut(s) 1034
Bsp1407I TGTACA 1 cut(s) 1315
BspANI GGCC 5 cut(s) 5, 100, 182, 426, 573
BspCNI CTCAG 2 cut(s) 259, 1660
BspEI TCCGGA 1 cut(s) 1034
BspFNI CGCG 3 cut(s) 388, 470, 716
BspLI GGNNCC 4 cut(s) 499, 526, 556, 1585
BspMI ACCTGC 1 cut(s) 1498
BspPI GGATC 5 cut(s) 248, 692, 1286, 1486, 1661
BspT104I TTCGAA 1 cut(s) 1002
BspT107I GGYRCC 2 cut(s) 524, 554
BspTNI GGTCTC 1 cut(s) 771
BsrBI CCGCTC 1 cut(s) 1829
BsrDI GCAATG 3 cut(s) 46, 1541, 1691
BsrFI RCCGGY 1 cut(s) 1816
BsrGI TGTACA 1 cut(s) 1315
BsrI ACTGG 2 cut(s) 447, 1444
BssAI RCCGGY 1 cut(s) 1816
BssECI CCNNGG 6 cut(s) 176, 177, 427, 520, 601, 684
BssNI GRCGYC 3 cut(s) 525, 555, 993
Bst2UI CCWGG 4 cut(s) 178, 830, 1581, 1602
Bst4CI ACNGT 8 cut(s) 161, 250, 541, 613, 855, 1071, 1260, 1824
Bst6I CTCTTC 1 cut(s) 1530
BstACI GRCGYC 3 cut(s) 525, 555, 993
BstAUI TGTACA 1 cut(s) 1315
BstBI TTCGAA 1 cut(s) 1002
BstC8I GCNNGC 2 cut(s) 392, 590
BstDEI CTNAG 3 cut(s) 267, 972, 1647
BstF5I GGATG 5 cut(s) 516, 694, 928, 932, 964
BstFNI CGCG 3 cut(s) 388, 470, 716
BstH2I RGCGCY 3 cut(s) 528, 558, 623
BstHHI GCGC 7 cut(s) 390, 414, 472, 527, 557, 622, 1783
BstMAI GTCTC 2 cut(s) 459, 771
BstMCI CGRYCG 3 cut(s) 369, 379, 574
BstNI CCWGG 4 cut(s) 178, 830, 1581, 1602
BstSCI CCNGG 7 cut(s) 176, 363, 520, 683, 828, 1579, 1600
BstSFI CTRYAG 2 cut(s) 784, 909
BstUI CGCG 3 cut(s) 388, 470, 716
BstV1I GCAGC 3 cut(s) 346, 1393, 1396
BstV2I GAAGAC 2 cut(s) 443, 1354
BstX2I RGATCY 2 cut(s) 435, 1151
BstXI CCANNNNNNTGG 1 cut(s) 544
BstYI RGATCY 2 cut(s) 435, 1151
BstZI CGGCCG 1 cut(s) 571
BsuI GTATCC 2 cut(s) 1183, 1556
BsuRI GGCC 5 cut(s) 5, 100, 182, 426, 573
BtrI CACGTC 1 cut(s) 358
BtsCI GGATG 5 cut(s) 516, 694, 928, 932, 964
BtsIMutI CAGTG 3 cut(s) 609, 778, 1820
BveI ACCTGC 1 cut(s) 1498
Cac8I GCNNGC 2 cut(s) 392, 590
CfoI GCGC 7 cut(s) 390, 414, 472, 527, 557, 622, 1783
Cfr10I RCCGGY 1 cut(s) 1816
Cfr13I GGNCC 7 cut(s) 99, 180, 188, 497, 583, 1178, 1583
CseI GACGC 4 cut(s) 476, 485, 957, 1001
CsiI ACCWGGT 1 cut(s) 1600
Csp6I GTAC 6 cut(s) 607, 722, 1256, 1316, 1371, 1473
CviAII CATG 3 cut(s) 502, 595, 1697
CviQI GTAC 6 cut(s) 607, 722, 1256, 1316, 1371, 1473
DdeI CTNAG 3 cut(s) 267, 972, 1647
DinI GGCGCC 2 cut(s) 526, 556
DrdI GACNNNNNNGTC 1 cut(s) 913
DseDI GACNNNNNNGTC 1 cut(s) 913
EaeI YGGCCR 2 cut(s) 3, 571
EagI CGGCCG 1 cut(s) 571
Eam1104I CTCTTC 1 cut(s) 1530
EarI CTCTTC 1 cut(s) 1530
EciI GGCGGA 4 cut(s) 109, 234, 1202, 1262
EclXI CGGCCG 1 cut(s) 571
Eco24I GRGCYC 2 cut(s) 375, 1344
Eco31I GGTCTC 1 cut(s) 771
Eco32I GATATC 1 cut(s) 1678
Eco47I GGWCC 5 cut(s) 188, 497, 583, 1178, 1583
Eco52I CGGCCG 1 cut(s) 571
Eco57I CTGAAG 1 cut(s) 1610
Eco88I CYCGRG 2 cut(s) 428, 562
EcoO109I RGGNCCY 1 cut(s) 497
EcoRI GAATTC 1 cut(s) 1004
EcoRII CCWGG 4 cut(s) 176, 828, 1579, 1600
EcoRV GATATC 1 cut(s) 1678
EcoT38I GRGCYC 2 cut(s) 375, 1344
EgeI GGCGCC 2 cut(s) 526, 556
EheI GGCGCC 2 cut(s) 526, 556
Esp3I CGTCTC 1 cut(s) 459
FaeI CATG 3 cut(s) 505, 598, 1700
FaqI GGGAC 2 cut(s) 958, 1569
FatI CATG 3 cut(s) 501, 594, 1696
FbaI TGATCA 2 cut(s) 637, 793
FblI GTMKAC 1 cut(s) 807
Fnu4HI GCNGC 9 cut(s) 52, 55, 58, 183, 217, 335, 717, 1382, 1385
FokI GGATG 5 cut(s) 503, 701, 935, 939, 971
FriOI GRGCYC 2 cut(s) 375, 1344
Fsp4HI GCNGC 9 cut(s) 52, 55, 58, 183, 217, 335, 717, 1382, 1385
FspBI CTAG 5 cut(s) 1124, 1218, 1301, 1415, 1832
GlaI GCGC 7 cut(s) 389, 413, 471, 526, 556, 621, 1782
GluI GCNGC 9 cut(s) 52, 55, 58, 183, 217, 335, 717, 1382, 1385
GsaI CCCAGC 2 cut(s) 341, 1533
HaeII RGCGCY 3 cut(s) 528, 558, 623
HaeIII GGCC 5 cut(s) 5, 100, 182, 426, 573
HapII CCGG 9 cut(s) 186, 365, 522, 574, 684, 1017, 1035, 1476, 1817
HgaI GACGC 4 cut(s) 476, 485, 957, 1001
HhaI GCGC 7 cut(s) 390, 414, 472, 527, 557, 622, 1783
Hin1I GRCGYC 3 cut(s) 525, 555, 993
Hin1II CATG 3 cut(s) 505, 598, 1700
Hin6I GCGC 7 cut(s) 388, 412, 470, 525, 555, 620, 1781
HinP1I GCGC 7 cut(s) 388, 412, 470, 525, 555, 620, 1781
HindIII AAGCTT 3 cut(s) 1079, 1428, 1720
HinfI GANTC 5 cut(s) 281, 914, 1221, 1538, 1700
HpaII CCGG 9 cut(s) 186, 365, 522, 574, 684, 1017, 1035, 1476, 1817
HphI GGTGA 5 cut(s) 637, 707, 808, 1006, 1039
Hpy166II GTNNAC 4 cut(s) 361, 609, 808, 1607
Hpy188I TCNGA 5 cut(s) 199, 381, 602, 1253, 1812
Hpy8I GTNNAC 4 cut(s) 361, 609, 808, 1607
Hpy99I CGWCG 2 cut(s) 461, 554
HpyAV CCTTC 8 cut(s) 201, 488, 1112, 1191, 1358, 1494, 1585, 1681
HpyCH4III ACNGT 8 cut(s) 161, 250, 541, 613, 855, 1071, 1260, 1824
HpyCH4IV ACGT 6 cut(s) 357, 760, 1333, 1369, 1612, 1743
HpyCH4V TGCA 4 cut(s) 758, 1381, 1696, 1709
HpyF3I CTNAG 3 cut(s) 267, 972, 1647
HpySE526I ACGT 6 cut(s) 357, 760, 1333, 1369, 1612, 1743
Hsp92I GRCGYC 3 cut(s) 525, 555, 993
Hsp92II CATG 3 cut(s) 505, 598, 1700
HspAI GCGC 7 cut(s) 388, 412, 470, 525, 555, 620, 1781
KasI GGCGCC 2 cut(s) 524, 554
Kpn2I TCCGGA 1 cut(s) 1034
Ksp22I TGATCA 2 cut(s) 637, 793
LmnI GCTCC 3 cut(s) 313, 603, 1504
Lsp1109I GCAGC 3 cut(s) 346, 1393, 1396
LweI GCATC 1 cut(s) 1012
MabI ACCWGGT 1 cut(s) 1600
MaeI CTAG 5 cut(s) 1124, 1218, 1301, 1415, 1832
MaeII ACGT 6 cut(s) 357, 760, 1333, 1369, 1612, 1743
MaeIII GTNAC 4 cut(s) 7, 679, 731, 855
MbiI CCGCTC 1 cut(s) 1829
MboII GAAGA 5 cut(s) 443, 1161, 1359, 1547, 1607
MflI RGATCY 2 cut(s) 435, 1151
MhlI GDGCHC 3 cut(s) 375, 1344, 1618
MluCI AATT 5 cut(s) 171, 288, 1004, 1164, 1205
Mly113I GGCGCC 2 cut(s) 525, 555
MlyI GAGTC 2 cut(s) 923, 1547
MmeI TCCRAC 1 cut(s) 359
MroI TCCGGA 1 cut(s) 1034
MroXI GAANNNNTTC 1 cut(s) 160
MseI TTAA 6 cut(s) 170, 291, 615, 1163, 1325, 1515
MspA1I CMGCKG 3 cut(s) 337, 394, 1659
MspI CCGG 9 cut(s) 186, 365, 522, 574, 684, 1017, 1035, 1476, 1817
MspR9I CCNGG 7 cut(s) 178, 365, 522, 685, 830, 1581, 1602
Mva1269I GAATGC 2 cut(s) 637, 824
MvaI CCWGG 4 cut(s) 178, 830, 1581, 1602
MvnI CGCG 3 cut(s) 388, 470, 716
NarI GGCGCC 2 cut(s) 525, 555
NciI CCSGG 3 cut(s) 365, 522, 685
NlaIII CATG 3 cut(s) 505, 598, 1700
NlaIV GGNNCC 4 cut(s) 499, 526, 556, 1585
NmeAIII GCCGAG 1 cut(s) 687
NmuCI GTSAC 2 cut(s) 7, 679
NspV TTCGAA 1 cut(s) 1002
PaeR7I CTCGAG 1 cut(s) 562
PasI CCCWGGG 1 cut(s) 177
PcsI WCGNNNNNNNCGW 1 cut(s) 999
PctI GAATGC 2 cut(s) 637, 824
PdmI GAANNNNTTC 1 cut(s) 160
PfeI GAWTC 3 cut(s) 281, 1221, 1700
Pfl23II CGTACG 1 cut(s) 1370
PfoI TCCNGGA 1 cut(s) 828
PkrI GCNGC 9 cut(s) 53, 56, 59, 184, 218, 336, 718, 1383, 1386
PleI GAGTC 2 cut(s) 922, 1546
PluTI GGCGCC 2 cut(s) 528, 558
PpsI GAGTC 2 cut(s) 922, 1546
PpuMI RGGWCCY 1 cut(s) 497
PshBI ATTAAT 2 cut(s) 291, 1163
Psp5II RGGWCCY 1 cut(s) 497
Psp6I CCWGG 4 cut(s) 176, 828, 1579, 1600
PspFI CCCAGC 2 cut(s) 337, 1529
PspGI CCWGG 4 cut(s) 176, 828, 1579, 1600
PspLI CGTACG 1 cut(s) 1370
PspN4I GGNNCC 4 cut(s) 499, 526, 556, 1585
PspPI GGNCC 7 cut(s) 99, 180, 188, 497, 583, 1178, 1583
PspPPI RGGWCCY 1 cut(s) 497
PspXI VCTCGAGB 1 cut(s) 562
PsuI RGATCY 2 cut(s) 435, 1151
PvuII CAGCTG 3 cut(s) 337, 394, 1659
RsaI GTAC 6 cut(s) 608, 723, 1257, 1317, 1372, 1474
RsaNI GTAC 6 cut(s) 607, 722, 1256, 1316, 1371, 1473
SaqAI TTAA 6 cut(s) 170, 291, 615, 1163, 1325, 1515
SatI GCNGC 9 cut(s) 52, 55, 58, 183, 217, 335, 717, 1382, 1385
Sau96I GGNCC 7 cut(s) 99, 180, 188, 497, 583, 1178, 1583
SchI GAGTC 2 cut(s) 923, 1547
ScrFI CCNGG 7 cut(s) 178, 365, 522, 685, 830, 1581, 1602
SduI GDGCHC 3 cut(s) 375, 1344, 1618
SexAI ACCWGGT 1 cut(s) 1600
SfaNI GCATC 1 cut(s) 1012
SfcI CTRYAG 2 cut(s) 784, 909
SfoI GGCGCC 2 cut(s) 526, 556
Sfr274I CTCGAG 1 cut(s) 562
SfuI TTCGAA 1 cut(s) 1002
SinI GGWCC 5 cut(s) 188, 497, 583, 1178, 1583
SlaI CTCGAG 1 cut(s) 562
SmlI CTYRAG 3 cut(s) 34, 439, 562
SmoI CTYRAG 3 cut(s) 34, 439, 562
Sse9I AATT 5 cut(s) 171, 288, 1004, 1164, 1205
SspDI GGCGCC 2 cut(s) 524, 554
SspMI CTAG 5 cut(s) 1124, 1218, 1301, 1415, 1832
StyD4I CCNGG 7 cut(s) 176, 363, 520, 683, 828, 1579, 1600
TaaI ACNGT 8 cut(s) 161, 250, 541, 613, 855, 1071, 1260, 1824
TaiI ACGT 6 cut(s) 360, 763, 1336, 1372, 1615, 1746
TaqI TCGA 6 cut(s) 399, 563, 1002, 1099, 1626, 1703
TaqII GACCGA 1 cut(s) 365
TasI AATT 5 cut(s) 171, 288, 1004, 1164, 1205
TatI WGTACW 1 cut(s) 1315
TauI GCSGC 6 cut(s) 54, 57, 60, 185, 219, 719
TfiI GAWTC 3 cut(s) 281, 1221, 1700
Tru1I TTAA 6 cut(s) 170, 291, 615, 1163, 1325, 1515
Tru9I TTAA 6 cut(s) 170, 291, 615, 1163, 1325, 1515
TscAI CASTG 3 cut(s) 616, 778, 1827
TseFI GTSAC 2 cut(s) 7, 679
TseI GCWGC 3 cut(s) 334, 1381, 1384
Tsp45I GTSAC 2 cut(s) 7, 679
TspDTI ATGAA 4 cut(s) 417, 1131, 1254, 1752
TspGWI ACGGA 1 cut(s) 1466
TspRI CASTG 3 cut(s) 616, 778, 1827
VpaK11BI GGWCC 5 cut(s) 188, 497, 583, 1178, 1583
VspI ATTAAT 2 cut(s) 291, 1163
XapI RAATTY 1 cut(s) 1004
XbaI TCTAGA 2 cut(s) 1217, 1831
XhoI CTCGAG 1 cut(s) 562
XmiI GTMKAC 1 cut(s) 807
XmnI GAANNNNTTC 1 cut(s) 160
XspI CTAG 5 cut(s) 1124, 1218, 1301, 1415, 1832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.