Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0005299

Physical Location & Seq

Genomic Coordinates Forward (+)

20527685 .. 20533600

Gene Structure 5916 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1210500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2955 bp

ATGAGCACGCAGAGGCAGCATGTGGCCGTCCGGGACCTCGTTGAGGAAGCCAAGAAGCGGATTGTGTTTCTCGCCATTTGCGTTGTTGGCTTGTCATACCTTATGTCCCTGACGAGCTCCTCTGTCTGGGTGAACTTGCCTGCTGCCGCCTCGTTGATTGTCATCGTTCGTTATTTGTCACTAGATTATGACATGCGAAGAAAAGCTGCATCATACAACAGCAAGCCACCATCAGCAAATACTACTTCTCAAAACAAACCTATTAAATGGCCTAAAACTAGTCAAAAGTCTGAGTGGAGAAGGAAAGTTAATTCTCCTGTTGTCGAGGAAGCTATTGATCACTTCACCTGGCATCTAGTTTCTGAGTTTGTGACCGATCTCTGGTATTCTCGTCTAACACCGGATAGACAAGGTCCAGAAGAACTCGCGTGCATCATGAATGGTGTTCTTGGGGAAATTTCAGCACGCATGAGGAATATAAATCTAATTGATCTTCTCACAAGGGATCTTATTAATCTCATATGCTCTCACTTGGAGCTTTTCCGCATAGCTCAAGCAAAGATTCAAAAGCAACAATCGGGATGCCTGACAGTTGAAAAACGAGATATGGAAATAAGACTTATCTTGTCTGCTGAAAACAAATTGCACCCTGCTTTATTTTCTGCTGAATCTGAGCACAAGGTCTTGCAGCATTTGATGGATGGCCTGATCTCATTCTCATTCAGGCCTGAAGATCTGCAATGCACTCTCTTCCGTTATATTGTCAGAGAACTACTTGCTTGTGCAGTAATGCGACCTGTCCTAAACTTGGCTAGTCCCAGGTTTATTAATGAAAGAATTGAATTGTTGGTCATTAAAATGACCGAGCCTAAAGGTGTTATTGCGGTACAAGAGGCATCTCGGTCCAAACCAGAGGGGCCCTCAAAGTTATCATCTGATCACTTTTCTAGGTTCTTAGATACTTCTGTTACTGGTGTTGAACTTGTGCAATTAAAAAATGGTCAGTCCAGAACTGCTGTGGAGACAGCTGCAAATGTAAATGGATCAAAAGATCCATTGCTCTTGGTTGATACTCAATCTTCCCGTTCGTGGAGCTCAGTGCGAATGAACTCCCACACTAGCAATGAAAGGGGTGTAGAACAGAACCACTCGGGAGGAGAATGGGGAGATATGTTAGATCTGATGTCACGCAGAAAGACTCAAGCCCTTACTCCTGAAAATTTTGAAAACATGTGGGCTAAAGGGAGGAATTTTAGAAAGAAAGAAGGTGAAATAAATGAGCATTCTTCTGGTGGGAAGTCAGTTACAGTAGATCATTTTATGGAAAAATCTAGGCCCAAAGATAAGGAAAATGTTTCGAAGTTCAATATTTCTGATAGAGGCATTTCTCAAAACAATTTCCACCCTGGAGCTCAAAACATACCAAGTTACTCTCGAGGTTCTTCATATCAGGATGATGATGAACACAACCACATGCGGTTGGACGAGTTTGAATCAGGAAGCAGTACTGCTTATACTTCGGAAGATGAAGAAACAGACAGTGTTACAGGTCTTGATTCTCCTGGAACTAAAGTTTGGGATGGTAGAAGTAATAGAAATATGGCAATTTCTCACATTCATCACCCACTTGAAAATTCTGAACGCCATATTAGGAAACGGACTGGTAAAGGGAATCTTCACTTTAACAGATTGTCTAAAACCCAGTCTTTCCAGAAAAGATCTAGACCAAGCAACAAGAAAGTGCCTGTTTGGCAAGAGGTAGAGAGAAAACGCTTCTTGTCTGGAGATGGACAGGACATACTTAAATCTCCAAATCGAGATGCAAATATTGAGGACTCTAGCGATGATTCTGACGTTGAAAGTTTGGGTAGAATTAACAGTGGGGCAGCCACTTCTTCATCTGCAACTTTATCTTTTGCAGATAGTCACAGCCTGAATTTTAATTCCCTGAAAAATTCTCTAGCAGTGGATTCTTTTTTCAAGTTGAAATGTGAGGTATTGGGTGCAAATATTGTGAAGAGTGGCTCAAAAACATTTGCCGTTTACTCCATATCTGTTACGGATGTAAATAATAATAGTTGGTCAATCAAAAGAAGGTTTAGTCATTTTGAGGAGCTACATCGGCGTCTAAAAGAGTTTCCTGAGTACAACCTACACTTGCCACCAAAGCATTTTCTGTCAACTGGTTTGGATTTAGCTGTCATTCAGGAACGATGTAAATCACTTGACAAATATGTAAAGAAACTTATGCAGCTTCCAAGAGTTTCAGGATCCATTGAAGTTTGGGACTTTCTAAGTGTTGACTCCCAGACTTATCTATTTACAAATTCCTTTTCTATAATCGAAACACTGTCAGTTGACCTAGATGATAAACCATCTGAAAAGAGCAAAAGGGTTTCAAATATGGATGGGCTGGTGACTGATCCGTTCTTGACGAGGGAACATACGGGCAATGGAGTTAAGGGTTCTGCTTTACAACTGAAGAATAATGATGGGTTAAGAGTGAACACAAAAGTTTCTGATTCCCAGGTAAAAAGTCCTGGTAAAGAAATTGGAAAGTCACTCATTAATTCTGGCACTGATTCAGATGCTAGAGCAAAAAAAGATTTATCTTCTGTCAAAAACTTGGGTAAGACCATAAAAGGAAGAGAGGAACAAGAATCTGAATCGTTTCTTGATACTGATACAGTCCCTACACTTCCTACAGAGTGGGTACCTCCAAATTTAAGTGTTCCCATATTAGATTTGGTAGATGTCATTTTTCAGCTCCAAGATGGTGGATGGATCAGGCGAAAAGCTTTTTGGGTGGCCAAACAAATACTACAGCTAGGGATGGGTGATGCCTTCGATGACTGGTTAATGGAGAAAATTCAACTTCTGCGCAAGGGATTGGTGGTTGCTTCAGGGATCAAGCGTGTTGAGCAGGTACTTTTTGACGTCATTTGCTTCCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Nexin_C PX PXA

Protein Analysis

985

Amino Acids

110.87

Weight (kDa)

8.63

Isoelectric Point (pI)

45.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PXA	PF02194	105 - 281	2e-40	PXA domain
PX	PF00787	690 - 767	1.7e-13	PX domain
Nexin_C	PF08628	928 - 978	2.8e-07	Sorting nexin C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005299)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15240 AT1G15240 AT1G15240 AT1G15240 AT1G15240
fragaria_vesca	FvH4_6g31500 FvH4_6g31500 FvH4_6g31500 FvH4_6g31500 FvH4_6g31500 FvH4_6g31500 FvH4_6g31500
malus_domestica	MD09G1210500.v1.1 MD17G1193500.v1.1
prunus_persica	Prupe.3G068900_v2.0.a1 Prupe.3G068900_v2.0.a1 Prupe.3G068900_v2.0.a1
pyrus_communis	pycom09g12810 pycom17g19950
rosa_chinensis	RchiOBHm_Chr2g0138361
rosa_laevigata	RLG00000019739
rosa_multiflora	Rmu_sc0002475.1_g000001
rosa_roxburghii	Rroxscaffold_2G00106300
rosa_rugosa	Rorug02G0351700
rosa_samantha	Rh2AG400000 Rh2BG408400 Rh2CG386200 Rh2DG419700
rosa_wichuraiana	Rw2G032540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	2940
Acc16I	TGCGCA	1 cut(s)	2882
Acc36I	ACCTGC	1 cut(s)	2914
Acc65I	GGTACC	1 cut(s)	2713
AccB1I	GGYRCC	1 cut(s)	2713
AccII	CGCG	1 cut(s)	428
AciI	CCGC	5 cut(s)	58, 147, 544, 884, 1477
AclWI	GGATC	8 cut(s)	513, 1046, 1051, 2265, 2278, 2417, 2792, 2915
AcoI	YGGCCR	2 cut(s)	24, 2808
AcsI	RAATTY	9 cut(s)	456, 1219, 1249, 1633, 1936, 1954, 2326, 2722, 2868
AcuI	CTGAAG	3 cut(s)	750, 2501, 2886
AcyI	GRCGYC	2 cut(s)	2125, 2937
AfaI	GTAC	5 cut(s)	888, 1507, 2147, 2715, 2928
AfiI	CCNNNNNNNGG	6 cut(s)	43, 57, 126, 381, 808, 1089
AflIII	ACRYGT	1 cut(s)	1230
AhlI	ACTAGT	1 cut(s)	278
AjnI	CCWGG	6 cut(s)	347, 818, 1405, 1561, 2526, 2539
AjuI	GAANNNNNNNTTGG	4 cut(s)	1558, 1590, 2785, 2817
Alw21I	GWGCWC	5 cut(s)	8, 119, 678, 1097, 1414
Alw26I	GTCTC	1 cut(s)	1016
AlwI	GGATC	8 cut(s)	513, 1046, 1051, 2265, 2278, 2417, 2792, 2915
Ama87I	CYCGRG	2 cut(s)	1150, 1434
AoxI	GGCC	7 cut(s)	24, 269, 703, 725, 917, 1334, 2808
ApaI	GGGCCC	1 cut(s)	921
ApeKI	GCWGC	7 cut(s)	16, 143, 206, 688, 1028, 1886, 2251
ApoI	RAATTY	9 cut(s)	456, 1219, 1249, 1633, 1936, 1954, 2326, 2722, 2868
AseI	ATTAAT	3 cut(s)	513, 828, 2568
Asp700I	GAANNNNTTC	1 cut(s)	2670
Asp718I	GGTACC	1 cut(s)	2713
AspLEI	GCGC	1 cut(s)	2883
AspS9I	GGNCC	6 cut(s)	34, 413, 903, 917, 918, 1335
AsuC2I	CCSGG	1 cut(s)	32
AsuHPI	GGTGA	6 cut(s)	142, 337, 1280, 1613, 2428, 2849
AsuII	TTCGAA	1 cut(s)	1358
AvaI	CYCGRG	2 cut(s)	1150, 1434
AvaII	GGWCC	3 cut(s)	34, 413, 903
BaeGI	GKGCMC	1 cut(s)	921
BaeI	ACNNNNGTAYC	2 cut(s)	951, 984
BalI	TGGCCA	1 cut(s)	2810
BamHI	GGATCC	1 cut(s)	2270
BanI	GGYRCC	1 cut(s)	2713
BanII	GRGCYC	4 cut(s)	119, 921, 1097, 1414
Bbv12I	GWGCWC	5 cut(s)	8, 119, 678, 1097, 1414
BbvI	GCAGC	7 cut(s)	28, 130, 193, 700, 1015, 1898, 2263
BceAI	ACGGC	2 cut(s)	11, 2024
BciT130I	CCWGG	6 cut(s)	349, 820, 1407, 1563, 2528, 2541
BclI	TGATCA	2 cut(s)	337, 937
BcnI	CCSGG	1 cut(s)	32
BcoDI	GTCTC	1 cut(s)	1016
BcuI	ACTAGT	1 cut(s)	278
BfmI	CTRYAG	2 cut(s)	2703, 2822
BfuAI	ACCTGC	1 cut(s)	2914
BglI	GCCNNNNNGGC	1 cut(s)	1750
BglII	AGATCT	3 cut(s)	733, 1177, 1718
BisI	GCNGC	8 cut(s)	17, 144, 147, 207, 689, 1029, 1887, 2252
BlsI	GCNGC	8 cut(s)	18, 145, 148, 208, 690, 1030, 1888, 2253
BmcAI	AGTACT	1 cut(s)	1507
Bme1390I	CCNGG	7 cut(s)	32, 349, 820, 1407, 1563, 2528, 2541
Bme18I	GGWCC	3 cut(s)	34, 413, 903
BmeT110I	CYCGRG	2 cut(s)	1150, 1434
BmgT120I	GGNCC	6 cut(s)	34, 413, 903, 917, 918, 1335
BmiI	GGNNCC	5 cut(s)	35, 918, 919, 2272, 2715
BmrFI	CCNGG	7 cut(s)	32, 349, 820, 1407, 1563, 2528, 2541
BmrI	ACTGGG	1 cut(s)	1696
BmsI	GCATC	8 cut(s)	218, 361, 441, 572, 905, 1810, 2578, 2830
BmuI	ACTGGG	1 cut(s)	1696
BplI	GAGNNNNNCTC	2 cut(s)	905, 937
BpmI	CTGGAG	2 cut(s)	1428, 1803
Bpu14I	TTCGAA	1 cut(s)	1358
BpuEI	CTTGAG	2 cut(s)	537, 1185
BpuMI	CCSGG	1 cut(s)	32
BsaBI	GATNNNNATC	2 cut(s)	161, 2218
BsaHI	GRCGYC	2 cut(s)	2125, 2937
BsaJI	CCNNGG	3 cut(s)	818, 1405, 2526
BsaWI	WCCGGW	1 cut(s)	400
Bsc4I	CCNNNNNNNGG	6 cut(s)	43, 57, 126, 381, 808, 1089
Bse1I	ACTGG	5 cut(s)	976, 1666, 1702, 2188, 2858
Bse3DI	GCAATG	4 cut(s)	746, 1055, 1129, 2458
Bse8I	GATNNNNATC	2 cut(s)	161, 2218
BseBI	CCWGG	6 cut(s)	349, 820, 1407, 1563, 2528, 2541
BseDI	CCNNGG	3 cut(s)	818, 1405, 2526
BseGI	GGATG	8 cut(s)	587, 706, 1459, 1585, 2068, 2413, 2786, 2838
BseJI	GATNNNNATC	2 cut(s)	161, 2218
BseLI	CCNNNNNNNGG	6 cut(s)	43, 57, 126, 381, 808, 1089
BseMI	GCAATG	4 cut(s)	746, 1055, 1129, 2458
BseMII	CTCAG	5 cut(s)	282, 354, 663, 1110, 2133
BseNI	ACTGG	5 cut(s)	976, 1666, 1702, 2188, 2858
BseRI	GAGGAG	3 cut(s)	109, 1170, 2126
BseSI	GKGCMC	1 cut(s)	921
BseXI	GCAGC	7 cut(s)	28, 130, 193, 700, 1015, 1898, 2263
BsgI	GTGCAG	1 cut(s)	804
Bsh1236I	CGCG	1 cut(s)	428
BshFI	GGCC	7 cut(s)	26, 271, 705, 727, 919, 1336, 2810
BshNI	GGYRCC	1 cut(s)	2713
BsiHKAI	GWGCWC	5 cut(s)	8, 119, 678, 1097, 1414
BsiHKCI	CYCGRG	2 cut(s)	1150, 1434
BsiSI	CCGG	2 cut(s)	31, 401
BslFI	GGGAC	5 cut(s)	47, 91, 801, 2300, 2675
BslI	CCNNNNNNNGG	6 cut(s)	43, 57, 126, 381, 808, 1089
BsmAI	GTCTC	1 cut(s)	1016
BsmFI	GGGAC	5 cut(s)	47, 91, 801, 2300, 2675
BsmI	GAATGC	1 cut(s)	1282
BsnI	GGCC	7 cut(s)	26, 271, 705, 727, 919, 1336, 2810
BsoBI	CYCGRG	2 cut(s)	1150, 1434
Bsp119I	TTCGAA	1 cut(s)	1358
Bsp120I	GGGCCC	1 cut(s)	917
Bsp1286I	GDGCHC	6 cut(s)	8, 119, 678, 921, 1097, 1414
BspACI	CCGC	5 cut(s)	58, 147, 544, 884, 1477
BspANI	GGCC	7 cut(s)	26, 271, 705, 727, 919, 1336, 2810
BspCNI	CTCAG	5 cut(s)	283, 355, 664, 1109, 2134
BspFNI	CGCG	1 cut(s)	428
BspHI	TCATGA	1 cut(s)	435
BspLI	GGNNCC	5 cut(s)	35, 918, 919, 2272, 2715
BspMI	ACCTGC	1 cut(s)	2914
BspPI	GGATC	8 cut(s)	513, 1046, 1051, 2265, 2278, 2417, 2792, 2915
BspT104I	TTCGAA	1 cut(s)	1358
BspT107I	GGYRCC	1 cut(s)	2713
BsrDI	GCAATG	4 cut(s)	746, 1055, 1129, 2458
BsrI	ACTGG	5 cut(s)	976, 1666, 1702, 2188, 2858
BssECI	CCNNGG	3 cut(s)	818, 1405, 2526
BssNI	GRCGYC	2 cut(s)	2125, 2937
Bst2UI	CCWGG	6 cut(s)	349, 820, 1407, 1563, 2528, 2541
Bst4CI	ACNGT	6 cut(s)	592, 1309, 1541, 1880, 2352, 2689
Bst6I	CTCTTC	3 cut(s)	755, 2012, 2641
BstACI	GRCGYC	2 cut(s)	2125, 2937
BstBI	TTCGAA	1 cut(s)	1358
BstC8I	GCNNGC	5 cut(s)	8, 141, 224, 430, 466
BstDEI	CTNAG	7 cut(s)	291, 363, 672, 955, 1096, 2142, 2294
BstENI	CCTNNNNNAGG	1 cut(s)	41
BstF5I	GGATG	8 cut(s)	587, 706, 1459, 1585, 2068, 2413, 2786, 2838
BstFNI	CGCG	1 cut(s)	428
BstHHI	GCGC	1 cut(s)	2883
BstMAI	GTCTC	1 cut(s)	1016
BstMWI	GCNNNNNNNGC	6 cut(s)	16, 87, 1750, 2122, 2167, 2920
BstNI	CCWGG	6 cut(s)	349, 820, 1407, 1563, 2528, 2541
BstNSI	RCATGY	4 cut(s)	23, 196, 1234, 1477
BstSCI	CCNGG	7 cut(s)	30, 347, 818, 1405, 1561, 2526, 2539
BstSFI	CTRYAG	2 cut(s)	2703, 2822
BstSLI	GKGCMC	1 cut(s)	921
BstUI	CGCG	1 cut(s)	428
BstV1I	GCAGC	7 cut(s)	28, 130, 193, 700, 1015, 1898, 2263
BstX2I	RGATCY	6 cut(s)	505, 733, 1051, 1177, 1718, 2270
BstXI	CCANNNNNNTGG	1 cut(s)	2777
BstYI	RGATCY	6 cut(s)	505, 733, 1051, 1177, 1718, 2270
BsuRI	GGCC	7 cut(s)	26, 271, 705, 727, 919, 1336, 2810
BtgZI	GCGATG	1 cut(s)	1857
BtsCI	GGATG	8 cut(s)	587, 706, 1459, 1585, 2068, 2413, 2786, 2838
BtsI	GCAGTG	1 cut(s)	1971
BtsIMutI	CAGTG	6 cut(s)	1104, 1546, 1885, 1971, 2348, 2577
BveI	ACCTGC	1 cut(s)	2914
Cac8I	GCNNGC	5 cut(s)	8, 141, 224, 430, 466
CciI	TCATGA	1 cut(s)	435
CfoI	GCGC	1 cut(s)	2883
Cfr13I	GGNCC	6 cut(s)	34, 413, 903, 917, 918, 1335
CseI	GACGC	1 cut(s)	2114
Csp6I	GTAC	5 cut(s)	887, 1506, 2146, 2714, 2927
CviAII	CATG	6 cut(s)	20, 193, 436, 469, 1231, 1474
CviQI	GTAC	5 cut(s)	887, 1506, 2146, 2714, 2927
DdeI	CTNAG	7 cut(s)	291, 363, 672, 955, 1096, 2142, 2294
EaeI	YGGCCR	2 cut(s)	24, 2808
Eam1104I	CTCTTC	3 cut(s)	755, 2012, 2641
EarI	CTCTTC	3 cut(s)	755, 2012, 2641
Ecl136II	GAGCTC	3 cut(s)	117, 1095, 1412
Eco147I	AGGCCT	1 cut(s)	727
Eco24I	GRGCYC	4 cut(s)	119, 921, 1097, 1414
Eco47I	GGWCC	3 cut(s)	34, 413, 903
Eco53kI	GAGCTC	3 cut(s)	117, 1095, 1412
Eco57I	CTGAAG	3 cut(s)	750, 2501, 2886
Eco88I	CYCGRG	2 cut(s)	1150, 1434
EcoICRI	GAGCTC	3 cut(s)	117, 1095, 1412
EcoNI	CCTNNNNNAGG	1 cut(s)	41
EcoO109I	RGGNCCY	3 cut(s)	34, 917, 918
EcoRII	CCWGG	6 cut(s)	347, 818, 1405, 1561, 2526, 2539
EcoT38I	GRGCYC	4 cut(s)	119, 921, 1097, 1414
FaeI	CATG	6 cut(s)	23, 196, 439, 472, 1234, 1477
FalI	AAGNNNNNCTT	2 cut(s)	1659, 1691
FaqI	GGGAC	5 cut(s)	47, 91, 801, 2300, 2675
FatI	CATG	6 cut(s)	19, 192, 435, 468, 1230, 1473
FauNDI	CATATG	1 cut(s)	521
FbaI	TGATCA	2 cut(s)	337, 937
Fnu4HI	GCNGC	8 cut(s)	17, 144, 147, 207, 689, 1029, 1887, 2252
FokI	GGATG	8 cut(s)	594, 713, 1466, 1592, 2075, 2420, 2793, 2845
FriOI	GRGCYC	4 cut(s)	119, 921, 1097, 1414
Fsp4HI	GCNGC	8 cut(s)	17, 144, 147, 207, 689, 1029, 1887, 2252
FspI	TGCGCA	1 cut(s)	2882
GlaI	GCGC	1 cut(s)	2882
GluI	GCNGC	8 cut(s)	17, 144, 147, 207, 689, 1029, 1887, 2252
GsuI	CTGGAG	2 cut(s)	1428, 1803
HaeIII	GGCC	7 cut(s)	26, 271, 705, 727, 919, 1336, 2810
HapII	CCGG	2 cut(s)	31, 401
HgaI	GACGC	1 cut(s)	2114
HhaI	GCGC	1 cut(s)	2883
Hin1I	GRCGYC	2 cut(s)	2125, 2937
Hin1II	CATG	6 cut(s)	23, 196, 439, 472, 1234, 1477
Hin6I	GCGC	1 cut(s)	2881
HinP1I	GCGC	1 cut(s)	2881
HincII	GTYRAC	3 cut(s)	2181, 2302, 2359
HindII	GTYRAC	3 cut(s)	2181, 2302, 2359
HindIII	AAGCTT	1 cut(s)	2796
HpaII	CCGG	2 cut(s)	31, 401
HphI	GGTGA	6 cut(s)	142, 337, 1280, 1613, 2428, 2849
Hpy166II	GTNNAC	6 cut(s)	133, 2044, 2181, 2302, 2359, 2506
Hpy8I	GTNNAC	6 cut(s)	133, 2044, 2181, 2302, 2359, 2506
HpyAV	CCTTC	4 cut(s)	294, 1259, 2088, 2854
HpyCH4III	ACNGT	6 cut(s)	592, 1309, 1541, 1880, 2352, 2689
HpyCH4IV	ACGT	2 cut(s)	1854, 2937
HpyF10VI	GCNNNNNNNGC	6 cut(s)	16, 87, 1750, 2122, 2167, 2920
HpyF3I	CTNAG	7 cut(s)	291, 363, 672, 955, 1096, 2142, 2294
HpySE526I	ACGT	2 cut(s)	1854, 2937
Hsp92I	GRCGYC	2 cut(s)	2125, 2937
Hsp92II	CATG	6 cut(s)	23, 196, 439, 472, 1234, 1477
HspAI	GCGC	1 cut(s)	2881
KpnI	GGTACC	1 cut(s)	2717
Ksp22I	TGATCA	2 cut(s)	337, 937
LmnI	GCTCC	6 cut(s)	122, 535, 1092, 1409, 2113, 2772
Lsp1109I	GCAGC	7 cut(s)	28, 130, 193, 700, 1015, 1898, 2263
LweI	GCATC	8 cut(s)	218, 361, 441, 572, 905, 1810, 2578, 2830
MaeII	ACGT	2 cut(s)	1854, 2937
MflI	RGATCY	6 cut(s)	505, 733, 1051, 1177, 1718, 2270
MhlI	GDGCHC	6 cut(s)	8, 119, 678, 921, 1097, 1414
MlsI	TGGCCA	1 cut(s)	2810
MluNI	TGGCCA	1 cut(s)	2810
MlyI	GAGTC	3 cut(s)	1192, 1829, 2297
MmeI	TCCRAC	1 cut(s)	1461
Mox20I	TGGCCA	1 cut(s)	2810
MroXI	GAANNNNTTC	1 cut(s)	2670
MscI	TGGCCA	1 cut(s)	2810
MslI	CAYNNNNRTG	1 cut(s)	857
Msp20I	TGGCCA	1 cut(s)	2810
MspA1I	CMGCKG	1 cut(s)	1028
MspI	CCGG	2 cut(s)	31, 401
MspR9I	CCNGG	7 cut(s)	32, 349, 820, 1407, 1563, 2528, 2541
Mva1269I	GAATGC	1 cut(s)	1282
MvaI	CCWGG	6 cut(s)	349, 820, 1407, 1563, 2528, 2541
MvnI	CGCG	1 cut(s)	428
MwoI	GCNNNNNNNGC	6 cut(s)	16, 87, 1750, 2122, 2167, 2920
NciI	CCSGG	1 cut(s)	32
NdeI	CATATG	1 cut(s)	521
NlaIII	CATG	6 cut(s)	23, 196, 439, 472, 1234, 1477
NlaIV	GGNNCC	5 cut(s)	35, 918, 919, 2272, 2715
NmuCI	GTSAC	6 cut(s)	177, 370, 1185, 1925, 2416, 2559
NsbI	TGCGCA	1 cut(s)	2882
NspI	RCATGY	4 cut(s)	23, 196, 1234, 1477
NspV	TTCGAA	1 cut(s)	1358
PaeR7I	CTCGAG	1 cut(s)	1434
PagI	TCATGA	1 cut(s)	435
PceI	AGGCCT	1 cut(s)	727
PciI	ACATGT	1 cut(s)	1230
PcsI	WCGNNNNNNNCGW	1 cut(s)	78
PctI	GAATGC	1 cut(s)	1282
PdmI	GAANNNNTTC	1 cut(s)	2670
PflFI	GACNNNGTC	1 cut(s)	411
PfoI	TCCNGGA	2 cut(s)	30, 1561
PkrI	GCNGC	8 cut(s)	18, 145, 148, 208, 690, 1030, 1888, 2253
PleI	GAGTC	3 cut(s)	1192, 1829, 2297
PpsI	GAGTC	3 cut(s)	1192, 1829, 2297
PpuMI	RGGWCCY	1 cut(s)	34
PscI	ACATGT	1 cut(s)	1230
PshBI	ATTAAT	3 cut(s)	513, 828, 2568
Psp124BI	GAGCTC	3 cut(s)	119, 1097, 1414
Psp5II	RGGWCCY	1 cut(s)	34
Psp6I	CCWGG	6 cut(s)	347, 818, 1405, 1561, 2526, 2539
PspGI	CCWGG	6 cut(s)	347, 818, 1405, 1561, 2526, 2539
PspN4I	GGNNCC	5 cut(s)	35, 918, 919, 2272, 2715
PspOMI	GGGCCC	1 cut(s)	917
PspPI	GGNCC	6 cut(s)	34, 413, 903, 917, 918, 1335
PspPPI	RGGWCCY	1 cut(s)	34
PsuI	RGATCY	6 cut(s)	505, 733, 1051, 1177, 1718, 2270
PsyI	GACNNNGTC	1 cut(s)	411
PvuII	CAGCTG	1 cut(s)	1028
RsaI	GTAC	5 cut(s)	888, 1507, 2147, 2715, 2928
RsaNI	GTAC	5 cut(s)	887, 1506, 2146, 2714, 2927
RseI	CAYNNNNRTG	1 cut(s)	857
SacI	GAGCTC	3 cut(s)	119, 1097, 1414
SatI	GCNGC	8 cut(s)	17, 144, 147, 207, 689, 1029, 1887, 2252
Sau96I	GGNCC	6 cut(s)	34, 413, 903, 917, 918, 1335
ScaI	AGTACT	1 cut(s)	1507
SchI	GAGTC	3 cut(s)	1192, 1829, 2297
ScrFI	CCNGG	7 cut(s)	32, 349, 820, 1407, 1563, 2528, 2541
SduI	GDGCHC	6 cut(s)	8, 119, 678, 921, 1097, 1414
SfaNI	GCATC	8 cut(s)	218, 361, 441, 572, 905, 1810, 2578, 2830
SfcI	CTRYAG	2 cut(s)	2703, 2822
Sfr274I	CTCGAG	1 cut(s)	1434
SfuI	TTCGAA	1 cut(s)	1358
SinI	GGWCC	3 cut(s)	34, 413, 903
SlaI	CTCGAG	1 cut(s)	1434
SmiMI	CAYNNNNRTG	1 cut(s)	857
SmlI	CTYRAG	3 cut(s)	552, 1200, 1434
SmoI	CTYRAG	3 cut(s)	552, 1200, 1434
SpeI	ACTAGT	1 cut(s)	278
SseBI	AGGCCT	1 cut(s)	727
SsiI	CCGC	5 cut(s)	58, 147, 544, 884, 1477
SspI	AATATT	3 cut(s)	1369, 1828, 2011
SstI	GAGCTC	3 cut(s)	119, 1097, 1414
StuI	AGGCCT	1 cut(s)	727
StyD4I	CCNGG	7 cut(s)	30, 347, 818, 1405, 1561, 2526, 2539
TaaI	ACNGT	6 cut(s)	592, 1309, 1541, 1880, 2352, 2689
TaiI	ACGT	2 cut(s)	1857, 2940
TaqI	TCGA	6 cut(s)	324, 1358, 1435, 1816, 2343, 2847
TaqII	GACCGA	3 cut(s)	389, 878, 891
TatI	WGTACW	2 cut(s)	1505, 2145
TauI	GCSGC	1 cut(s)	149
TscAI	CASTG	6 cut(s)	1104, 1546, 1885, 1971, 2355, 2584
TseFI	GTSAC	6 cut(s)	177, 370, 1185, 1925, 2416, 2559
TseI	GCWGC	7 cut(s)	16, 143, 206, 688, 1028, 1886, 2251
Tsp45I	GTSAC	6 cut(s)	177, 370, 1185, 1925, 2416, 2559
TspDTI	ATGAA	9 cut(s)	452, 846, 1121, 1140, 1434, 1476, 1542, 1607, 1887
TspGWI	ACGGA	4 cut(s)	743, 1672, 2075, 2415
TspRI	CASTG	6 cut(s)	1104, 1546, 1885, 1971, 2355, 2584
Tth111I	GACNNNGTC	1 cut(s)	411
VpaK11BI	GGWCC	3 cut(s)	34, 413, 903
VspI	ATTAAT	3 cut(s)	513, 828, 2568
XagI	CCTNNNNNAGG	1 cut(s)	41
XapI	RAATTY	9 cut(s)	456, 1219, 1249, 1633, 1936, 1954, 2326, 2722, 2868
XbaI	TCTAGA	1 cut(s)	1721
XceI	RCATGY	4 cut(s)	23, 196, 1234, 1477
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1015, 2743
XhoI	CTCGAG	1 cut(s)	1434
XmnI	GAANNNNTTC	1 cut(s)	2670
ZraI	GACGTC	1 cut(s)	2938
ZrmI	AGTACT	1 cut(s)	1507

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.