Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0008464

Physical Location & Seq

Genomic Coordinates Forward (+)

22304941 .. 22309896

Gene Structure 4956 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1218700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1950 bp

ATGGCGAAGGGAGGTTACTTCTTGGAGAAGGTTCGGAGATGCTTACGGACGGTGTTCTTCATGGTGGCGATGGTGGCGTCGCTGCTAGTGTCGTCGCTGCCGGCGCTGGTGGCGATAGGGGACATGCTGGTGCCGTGCGTGTTGATATCGAGCTTCACGTGCGTGACGTGCTACGGATTCAAAGAGCATTTGCATCGATACGCCTTCAAGAGCTCGCTCACCGATATTCCTCTGGTTTCCTTTATCAGATCTCTTATCATTATCTGTGTGCATTCCATGTGCGATGGCCCTGCTCTCTCACACGGTCCATACCTTGGAACTGTGACTTTCTGTTCCTTCATCTCAATTCTACTTCTTTCAATCAAAGCTTGTCTTTTCACTGTAAATTCTCAAATTGAGGCTGAAGCTTCGTCTTCCCTCTCAAGGCAGAGACTCCACTTGAAGAAGTCATGGGGAATGCCTGTCTTGTTTCTCTCATCAGTAGTCTTTTCCCTTGGACATACTGTGGTTGCTTATAGAACAAGTTGCAGAGCAAGGAGAAAGCTCCTATTTCACCGAGTTGATCCAGAAACTGTCCTTTCATGCAAAAATGTATTCTCTGGCTATCAGAAGGTCCCAAGATCTCCCACTCCCTCGGGAGGAAAAACCCCAAAAAGTGACAGTGAAATGAGGCGTAAGCCTTTTTCTACAGCTCGAGATGATGGTGAACTCCCAGTCAGAGTAATTACAGATATTGACAGCTTATTCATCACGTGCAGGGGGCTTACTCTTCATTACAAGCTTAGCTTGCCTGGTTCACCACCTCATTCCTTGTCATCCATTGCATTTCTTGAACCTAGCTCCCCAAAAATGGCTATGGGGAGGCCAAAATTAGATCGACATCCATTAAGCTTGTTATCGAAAGGCCAAAACCATCTCCACAGGAGCTACAGTAATCAATTTCACGGCTCATCTCTCTATGTACCTTTATTGGACGGTTCTACAGTTTCTCCTGTTCTTTCTGAAGAGATTCCTGTCTTGAGACTATACAATGCTGGTGAAGAGGATGAGGGTAGTAAATTAAATTTTGGTACTCCAAACAAAGAAATGGAAGGAAGTGACCAGTTTGGTATTCTATTAGTGCATGGGTTTGGGGGAGGAGCCTTTTCTTGGAGGCATGTGATGGGGACCCTTTCCCGGCAAGTTGGTTGCACAGTTGCTGCTTTTGATCGCCCTGGTTGGGGCCTAACCTCAAGGCTGCGACGGGAAGATTGGGAGGATAAAGAAATGCCTAATCCTTATAAACTTGATTCTCAAGTTGACCTGCTTCTTTCTTTCTGCTCTGAGATGGGATTTTCTTCAGTGGTGCTTGCTGGTCATGATGATGGAGGCCTTCTAGCTTTGATGGCTGCACAAAAAGTACAAGCATCAGTGAATTCTTTCAATGTTACAATTAAAGGCGTAGTATTGTTAAATGTTAGCTTGTCAAGAGAAGTTGTGCCTGCTTTTGCGAGGATACTCTTGAGAACTTCACTAGGGAAAAAGCACCTGGTTCGTCCATTACTGCGAACAGAAATTACACAAGTGGTGAATAGGCGTGCCTGGTACGATGCAACAAAGCTGACAACAGACATTTTGAGCCTTTATAAGGCCCCTCTATGTGTAGAAGGATGGGATGAAGCACTCCATGAGATAGGTAGACTGTCATACGAGACACTACTTTCACCGAAAAATGCAGAATCATTACTAAAGGCAGTTGAAGACATGCCAGTTTTAGTCATCGCAGGCGCTGAGGATTCCCTTGTCTCTCTTAAATCTTCTCAAGCTATGGCTTCCAAACTTGTAAATTCTAGACTAGTTGCAATATCCGGATGTGGCCATCTTCCCCACGAAGAGTGTCCCAAGGCATTACTTGCAGCAATAACACCCTTCTTAAGCGGACTCTTGAGCAGACAAGACGTGCAGAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0016787

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Abhydrolase_6

Protein Analysis

650

Amino Acids

71.18

Weight (kDa)

9.04

Isoelectric Point (pI)

47.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Abhydrolase_6	PF12697	371 - 632	5.9e-22	Alpha/beta hydrolase family
Abhydrolase_1	PF00561	371 - 625	8e-19	alpha/beta hydrolase fold
Hydrolase_4	PF12146	371 - 625	1.5e-07	Serine aminopeptidase, S33

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008464)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15490 AT1G15490 AT1G15490 AT1G15490 AT1G80280
fragaria_vesca	FvH4_6g30590
malus_domestica	MD09G1218700.v1.1 MD17G1202000.v1.1
prunus_persica	Prupe.3G077700_v2.0.a1 Prupe.3G077700_v2.0.a1
pyrus_communis	pycom17g20630 pycom17g20640
rosa_chinensis	RchiOBHm_Chr2g0137001
rosa_laevigata	RLG00000019594
rosa_multiflora	Rmu_sc0001374.1_g000058
rosa_roxburghii	Rroxscaffold_2G00107780
rosa_rugosa	Rorug02G0337000 Rorug02G0337100
rosa_samantha	Rh2AG386000 Rh2BG392900 Rh2CG373400 Rh2DG409100
rosa_wichuraiana	Rw2G031590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1281, 1626
Acc36I	ACCTGC	1 cut(s)	1311
AccB1I	GGYRCC	1 cut(s)	130
AccB7I	CCANNNNNTGG	1 cut(s)	314
AccI	GTMKAC	1 cut(s)	1678
AccIII	TCCGGA	1 cut(s)	1847
AciI	CCGC	1 cut(s)	1917
AclWI	GGATC	1 cut(s)	557
AcoI	YGGCCR	1 cut(s)	1855
AcsI	RAATTY	4 cut(s)	385, 1063, 1414, 1825
AcuI	CTGAAG	3 cut(s)	423, 1023, 1323
AcvI	CACGTG	2 cut(s)	159, 753
AcyI	GRCGYC	1 cut(s)	77
AfaI	GTAC	4 cut(s)	963, 1072, 1401, 1586
AfiI	CCNNNNNNNGG	9 cut(s)	314, 423, 638, 850, 1149, 1176, 1219, 1220, 1627
AflII	CTTAAG	1 cut(s)	1912
AgsI	TTSAA	7 cut(s)	181, 208, 360, 442, 833, 1423, 1739
AhlI	ACTAGT	1 cut(s)	1834
AjiI	CACGTC	2 cut(s)	168, 1939
AjnI	CCWGG	4 cut(s)	790, 1213, 1527, 1580
AleI	CACNNNNGTG	1 cut(s)	161
Alw21I	GWGCWC	1 cut(s)	215
Alw26I	GTCTC	4 cut(s)	424, 1015, 1685, 1789
AlwI	GGATC	1 cut(s)	557
AlwNI	CAGNNNCTG	3 cut(s)	572, 1199, 1769
Ama87I	CYCGRG	2 cut(s)	634, 693
Aor13HI	TCCGGA	1 cut(s)	1847
AoxI	GGCC	7 cut(s)	286, 863, 904, 1222, 1369, 1629, 1855
ApeKI	GCWGC	6 cut(s)	82, 97, 1199, 1237, 1388, 1895
ApoI	RAATTY	4 cut(s)	385, 1063, 1414, 1825
Asp700I	GAANNNNTTC	2 cut(s)	1008, 1418
AspLEI	GCGC	2 cut(s)	106, 1769
AspS9I	GGNCC	6 cut(s)	287, 305, 613, 1167, 1222, 1630
AsuC2I	CCSGG	1 cut(s)	1177
AsuHPI	GGTGA	7 cut(s)	211, 545, 716, 789, 1049, 1579, 1695
AvaI	CYCGRG	2 cut(s)	634, 693
AvaII	GGWCC	3 cut(s)	305, 613, 1167
BalI	TGGCCA	1 cut(s)	1857
BanI	GGYRCC	1 cut(s)	130
BanII	GRGCYC	1 cut(s)	215
BarI	GAAGNNNNNNTAC	1 cut(s)	31
BbrPI	CACGTG	2 cut(s)	159, 753
BbsI	GAAGAC	2 cut(s)	405, 1746
Bbv12I	GWGCWC	1 cut(s)	215
BbvCI	CCTCAGC	1 cut(s)	1770
BbvI	GCAGC	6 cut(s)	69, 84, 1186, 1224, 1375, 1907
BceAI	ACGGC	2 cut(s)	118, 961
BcgI	CGANNNNNNTGC	6 cut(s)	176, 210, 272, 306, 1514, 1548
BciT130I	CCWGG	4 cut(s)	792, 1215, 1529, 1582
BciVI	GTATCC	1 cut(s)	1488
BcnI	CCSGG	1 cut(s)	1177
BcoDI	GTCTC	4 cut(s)	424, 1015, 1685, 1789
BcuI	ACTAGT	1 cut(s)	1834
BfaI	CTAG	6 cut(s)	86, 837, 1376, 1514, 1830, 1835
BfmI	CTRYAG	3 cut(s)	687, 928, 981
BfoI	RGCGCY	2 cut(s)	107, 1770
BfrI	CTTAAG	1 cut(s)	1912
BfuAI	ACCTGC	1 cut(s)	1311
BfuI	GTATCC	1 cut(s)	1488
BglII	AGATCT	2 cut(s)	248, 620
BisI	GCNGC	6 cut(s)	83, 98, 1200, 1238, 1389, 1896
BlpI	GCTNAGC	1 cut(s)	782
BlsI	GCNGC	6 cut(s)	84, 99, 1201, 1239, 1390, 1897
Bme1390I	CCNGG	5 cut(s)	792, 1177, 1215, 1529, 1582
Bme18I	GGWCC	3 cut(s)	305, 613, 1167
BmeT110I	CYCGRG	2 cut(s)	634, 693
BmgBI	CACGTC	2 cut(s)	168, 1939
BmgT120I	GGNCC	6 cut(s)	287, 305, 613, 1167, 1222, 1630
BmiI	GGNNCC	7 cut(s)	132, 615, 1141, 1168, 1169, 1223, 1632
BmrFI	CCNGG	5 cut(s)	792, 1177, 1215, 1529, 1582
BmrI	ACTGGG	1 cut(s)	707
BmsI	GCATC	4 cut(s)	29, 202, 1415, 1579
BmuI	ACTGGG	1 cut(s)	707
BpiI	GAAGAC	2 cut(s)	405, 1746
Bpu10I	CCTNAGC	1 cut(s)	1770
Bpu1102I	GCTNAGC	1 cut(s)	782
BpuEI	CTTGAG	7 cut(s)	406, 1039, 1216, 1278, 1522, 1785, 1945
BpuMI	CCSGG	1 cut(s)	1177
Bsa29I	ATCGAT	1 cut(s)	196
BsaAI	YACGTR	2 cut(s)	159, 753
BsaBI	GATNNNNATC	1 cut(s)	879
BsaHI	GRCGYC	1 cut(s)	77
BsaJI	CCNNGG	5 cut(s)	313, 493, 633, 1213, 1881
BsaWI	WCCGGW	1 cut(s)	1847
BsaXI	ACNNNNNCTCC	4 cut(s)	916, 946, 973, 1003
Bsc4I	CCNNNNNNNGG	9 cut(s)	314, 423, 638, 850, 1149, 1176, 1219, 1220, 1627
Bse118I	RCCGGY	1 cut(s)	100
Bse1I	ACTGG	3 cut(s)	713, 1102, 1748
Bse3DI	GCAATG	1 cut(s)	819
Bse8I	GATNNNNATC	1 cut(s)	879
BseAI	TCCGGA	1 cut(s)	1847
BseBI	CCWGG	4 cut(s)	792, 1215, 1529, 1582
BseCI	ATCGAT	1 cut(s)	196
BseDI	CCNNGG	5 cut(s)	313, 493, 633, 1213, 1881
BseGI	GGATG	6 cut(s)	815, 880, 1051, 1655, 1660, 1856
BseJI	GATNNNNATC	1 cut(s)	879
BseLI	CCNNNNNNNGG	9 cut(s)	314, 423, 638, 850, 1149, 1176, 1219, 1220, 1627
BseMI	GCAATG	1 cut(s)	819
BseMII	CTCAG	2 cut(s)	1314, 1761
BseNI	ACTGG	3 cut(s)	713, 1102, 1748
BseRI	GAGGAG	1 cut(s)	1152
BseXI	GCAGC	6 cut(s)	69, 84, 1186, 1224, 1375, 1907
BsgI	GTGCAG	2 cut(s)	775, 1374
BshFI	GGCC	7 cut(s)	288, 865, 906, 1224, 1371, 1631, 1857
BshNI	GGYRCC	1 cut(s)	130
BshVI	ATCGAT	1 cut(s)	196
BsiHKAI	GWGCWC	1 cut(s)	215
BsiHKCI	CYCGRG	2 cut(s)	634, 693
BsiSI	CCGG	3 cut(s)	101, 1177, 1848
BslFI	GGGAC	4 cut(s)	134, 599, 1180, 1863
BslI	CCNNNNNNNGG	9 cut(s)	314, 423, 638, 850, 1149, 1176, 1219, 1220, 1627
BsmAI	GTCTC	4 cut(s)	424, 1015, 1685, 1789
BsmFI	GGGAC	4 cut(s)	134, 599, 1180, 1863
BsmI	GAATGC	2 cut(s)	271, 462
BsnI	GGCC	7 cut(s)	288, 865, 906, 1224, 1371, 1631, 1857
BsoBI	CYCGRG	2 cut(s)	634, 693
Bsp1286I	GDGCHC	1 cut(s)	215
Bsp13I	TCCGGA	1 cut(s)	1847
Bsp143I	GATC	5 cut(s)	248, 562, 620, 874, 1207
Bsp1720I	GCTNAGC	1 cut(s)	782
BspACI	CCGC	1 cut(s)	1917
BspANI	GGCC	7 cut(s)	288, 865, 906, 1224, 1371, 1631, 1857
BspCNI	CTCAG	2 cut(s)	1315, 1762
BspDI	ATCGAT	1 cut(s)	196
BspEI	TCCGGA	1 cut(s)	1847
BspHI	TCATGA	1 cut(s)	1357
BspLI	GGNNCC	7 cut(s)	132, 615, 1141, 1168, 1169, 1223, 1632
BspMI	ACCTGC	1 cut(s)	1311
BspPI	GGATC	1 cut(s)	557
BspT107I	GGYRCC	1 cut(s)	130
BspTI	CTTAAG	1 cut(s)	1912
BsrDI	GCAATG	1 cut(s)	819
BsrFI	RCCGGY	1 cut(s)	100
BsrI	ACTGG	3 cut(s)	713, 1102, 1748
BssAI	RCCGGY	1 cut(s)	100
BssECI	CCNNGG	5 cut(s)	313, 493, 633, 1213, 1881
BssMI	GATC	5 cut(s)	248, 562, 620, 874, 1207
BssNI	GRCGYC	1 cut(s)	77
BssT1I	CCWWGG	3 cut(s)	313, 493, 1881
Bst2UI	CCWGG	4 cut(s)	792, 1215, 1529, 1582
Bst6I	CTCTTC	4 cut(s)	774, 999, 1035, 1866
BstACI	GRCGYC	1 cut(s)	77
BstAFI	CTTAAG	1 cut(s)	1912
BstAPI	GCANNNNNTGC	1 cut(s)	1892
BstBAI	YACGTR	2 cut(s)	159, 753
BstC8I	GCNNGC	7 cut(s)	102, 215, 788, 1350, 1482, 1578, 1765
BstDEI	CTNAG	3 cut(s)	782, 1323, 1770
BstENI	CCTNNNNNAGG	1 cut(s)	1625
BstF5I	GGATG	6 cut(s)	815, 880, 1051, 1655, 1660, 1856
BstH2I	RGCGCY	2 cut(s)	107, 1770
BstHHI	GCGC	2 cut(s)	106, 1769
BstKTI	GATC	5 cut(s)	251, 565, 623, 877, 1210
BstMAI	GTCTC	4 cut(s)	424, 1015, 1685, 1789
BstMBI	GATC	5 cut(s)	248, 562, 620, 874, 1207
BstMWI	GCNNNNNNNGC	8 cut(s)	74, 103, 110, 159, 168, 787, 1385, 1892
BstNI	CCWGG	4 cut(s)	792, 1215, 1529, 1582
BstNSI	RCATGY	3 cut(s)	127, 1160, 1747
BstSCI	CCNGG	5 cut(s)	790, 1175, 1213, 1527, 1580
BstSFI	CTRYAG	3 cut(s)	687, 928, 981
BstV1I	GCAGC	6 cut(s)	69, 84, 1186, 1224, 1375, 1907
BstV2I	GAAGAC	2 cut(s)	405, 1746
BstX2I	RGATCY	2 cut(s)	248, 620
BstYI	RGATCY	2 cut(s)	248, 620
Bsu15I	ATCGAT	1 cut(s)	196
BsuI	GTATCC	1 cut(s)	1488
BsuRI	GGCC	7 cut(s)	288, 865, 906, 1224, 1371, 1631, 1857
BsuTUI	ATCGAT	1 cut(s)	196
BtgZI	GCGATG	3 cut(s)	83, 297, 1744
BtrI	CACGTC	2 cut(s)	168, 1939
BtsCI	GGATG	6 cut(s)	815, 880, 1051, 1655, 1660, 1856
BtsIMutI	CAGTG	4 cut(s)	378, 667, 1347, 1416
BveI	ACCTGC	1 cut(s)	1311
Cac8I	GCNNGC	7 cut(s)	102, 215, 788, 1350, 1482, 1578, 1765
CaiI	CAGNNNCTG	3 cut(s)	572, 1199, 1769
CciI	TCATGA	1 cut(s)	1357
CfoI	GCGC	2 cut(s)	106, 1769
Cfr10I	RCCGGY	1 cut(s)	100
Cfr13I	GGNCC	6 cut(s)	287, 305, 613, 1167, 1222, 1630
ClaI	ATCGAT	1 cut(s)	196
CseI	GACGC	1 cut(s)	66
CsiI	ACCWGGT	1 cut(s)	1527
Csp6I	GTAC	4 cut(s)	962, 1071, 1400, 1585
CviQI	GTAC	4 cut(s)	962, 1071, 1400, 1585
DdeI	CTNAG	3 cut(s)	782, 1323, 1770
DpnI	GATC	5 cut(s)	250, 564, 622, 876, 1209
DpnII	GATC	5 cut(s)	248, 562, 620, 874, 1207
EaeI	YGGCCR	1 cut(s)	1855
Eam1104I	CTCTTC	4 cut(s)	774, 999, 1035, 1866
EarI	CTCTTC	4 cut(s)	774, 999, 1035, 1866
Ecl136II	GAGCTC	1 cut(s)	213
Eco130I	CCWWGG	3 cut(s)	313, 493, 1881
Eco147I	AGGCCT	1 cut(s)	1371
Eco24I	GRGCYC	1 cut(s)	215
Eco32I	GATATC	1 cut(s)	147
Eco47I	GGWCC	3 cut(s)	305, 613, 1167
Eco53kI	GAGCTC	1 cut(s)	213
Eco57I	CTGAAG	3 cut(s)	423, 1023, 1323
Eco72I	CACGTG	2 cut(s)	159, 753
Eco88I	CYCGRG	2 cut(s)	634, 693
EcoICRI	GAGCTC	1 cut(s)	213
EcoNI	CCTNNNNNAGG	1 cut(s)	1625
EcoO109I	RGGNCCY	4 cut(s)	613, 1167, 1222, 1630
EcoRI	GAATTC	1 cut(s)	1414
EcoRII	CCWGG	4 cut(s)	790, 1213, 1527, 1580
EcoRV	GATATC	1 cut(s)	147
EcoT14I	CCWWGG	3 cut(s)	313, 493, 1881
EcoT38I	GRGCYC	1 cut(s)	215
ErhI	CCWWGG	3 cut(s)	313, 493, 1881
FalI	AAGNNNNNCTT	4 cut(s)	357, 389, 770, 802
FaqI	GGGAC	4 cut(s)	134, 599, 1180, 1863
FblI	GTMKAC	1 cut(s)	1678
Fnu4HI	GCNGC	6 cut(s)	83, 98, 1200, 1238, 1389, 1896
FokI	GGATG	6 cut(s)	802, 867, 1058, 1662, 1667, 1863
FriOI	GRGCYC	1 cut(s)	215
Fsp4HI	GCNGC	6 cut(s)	83, 98, 1200, 1238, 1389, 1896
FspBI	CTAG	6 cut(s)	86, 837, 1376, 1514, 1830, 1835
GlaI	GCGC	2 cut(s)	105, 1768
GluI	GCNGC	6 cut(s)	83, 98, 1200, 1238, 1389, 1896
HaeII	RGCGCY	2 cut(s)	107, 1770
HaeIII	GGCC	7 cut(s)	288, 865, 906, 1224, 1371, 1631, 1857
HapII	CCGG	3 cut(s)	101, 1177, 1848
HgaI	GACGC	1 cut(s)	66
HhaI	GCGC	2 cut(s)	106, 1769
Hin1I	GRCGYC	1 cut(s)	77
Hin6I	GCGC	2 cut(s)	104, 1767
HinP1I	GCGC	2 cut(s)	104, 1767
HincII	GTYRAC	1 cut(s)	1300
HindII	GTYRAC	1 cut(s)	1300
HindIII	AAGCTT	4 cut(s)	366, 405, 779, 889
HinfI	GANTC	7 cut(s)	177, 432, 1009, 1289, 1718, 1775, 1920
HpaII	CCGG	3 cut(s)	101, 1177, 1848
HphI	GGTGA	7 cut(s)	211, 545, 716, 789, 1049, 1579, 1695
Hpy166II	GTNNAC	4 cut(s)	707, 797, 1300, 1679
Hpy188I	TCNGA	6 cut(s)	36, 248, 609, 719, 1003, 1324
Hpy8I	GTNNAC	4 cut(s)	707, 797, 1300, 1679
Hpy99I	CGWCG	3 cut(s)	82, 97, 1245
HpyAV	CCTTC	8 cut(s)	22, 214, 346, 604, 1085, 1382, 1640, 1918
HpyCH4IV	ACGT	4 cut(s)	158, 167, 752, 1938
HpyF10VI	GCNNNNNNNGC	8 cut(s)	74, 103, 110, 159, 168, 787, 1385, 1892
HpyF3I	CTNAG	3 cut(s)	782, 1323, 1770
HpySE526I	ACGT	4 cut(s)	158, 167, 752, 1938
Hsp92I	GRCGYC	1 cut(s)	77
HspAI	GCGC	2 cut(s)	104, 1767
KflI	GGGWCCC	1 cut(s)	1167
Kpn2I	TCCGGA	1 cut(s)	1847
KroI	GCCGGC	1 cut(s)	100
KroNI	GCCGGC	1 cut(s)	102
Kzo9I	GATC	5 cut(s)	248, 562, 620, 874, 1207
LmnI	GCTCC	4 cut(s)	549, 845, 924, 1139
Lsp1109I	GCAGC	6 cut(s)	69, 84, 1186, 1224, 1375, 1907
LweI	GCATC	4 cut(s)	29, 202, 1415, 1579
MabI	ACCWGGT	1 cut(s)	1527
MaeI	CTAG	6 cut(s)	86, 837, 1376, 1514, 1830, 1835
MaeII	ACGT	4 cut(s)	158, 167, 752, 1938
MaeIII	GTNAC	6 cut(s)	14, 163, 322, 656, 1097, 1426
MalI	GATC	5 cut(s)	250, 564, 622, 876, 1209
MboI	GATC	5 cut(s)	248, 562, 620, 874, 1207
MflI	RGATCY	2 cut(s)	248, 620
MhlI	GDGCHC	1 cut(s)	215
MlsI	TGGCCA	1 cut(s)	1857
MluNI	TGGCCA	1 cut(s)	1857
MlyI	GAGTC	2 cut(s)	426, 1914
Mox20I	TGGCCA	1 cut(s)	1857
MroI	TCCGGA	1 cut(s)	1847
MroNI	GCCGGC	1 cut(s)	100
MroXI	GAANNNNTTC	2 cut(s)	1008, 1418
MscI	TGGCCA	1 cut(s)	1857
MseI	TTAA	6 cut(s)	887, 1061, 1434, 1451, 1791, 1913
MslI	CAYNNNNRTG	3 cut(s)	128, 161, 1362
Msp20I	TGGCCA	1 cut(s)	1857
MspCI	CTTAAG	1 cut(s)	1912
MspI	CCGG	3 cut(s)	101, 1177, 1848
MspR9I	CCNGG	5 cut(s)	792, 1177, 1215, 1529, 1582
Mva1269I	GAATGC	2 cut(s)	271, 462
MvaI	CCWGG	4 cut(s)	792, 1215, 1529, 1582
MwoI	GCNNNNNNNGC	8 cut(s)	74, 103, 110, 159, 168, 787, 1385, 1892
NaeI	GCCGGC	1 cut(s)	102
NciI	CCSGG	1 cut(s)	1177
NdeII	GATC	5 cut(s)	248, 562, 620, 874, 1207
NgoMIV	GCCGGC	1 cut(s)	100
NlaIV	GGNNCC	7 cut(s)	132, 615, 1141, 1168, 1169, 1223, 1632
NmuCI	GTSAC	4 cut(s)	163, 322, 656, 1097
NspI	RCATGY	3 cut(s)	127, 1160, 1747
OliI	CACNNNNGTG	1 cut(s)	161
PaeR7I	CTCGAG	1 cut(s)	693
PagI	TCATGA	1 cut(s)	1357
PceI	AGGCCT	1 cut(s)	1371
PcsI	WCGNNNNNNNCGW	2 cut(s)	155, 164
PctI	GAATGC	2 cut(s)	271, 462
PdiI	GCCGGC	1 cut(s)	102
PdmI	GAANNNNTTC	2 cut(s)	1008, 1418
PfeI	GAWTC	5 cut(s)	177, 1009, 1289, 1718, 1775
PflMI	CCANNNNNTGG	1 cut(s)	314
PkrI	GCNGC	6 cut(s)	84, 99, 1201, 1239, 1390, 1897
PleI	GAGTC	2 cut(s)	426, 1914
PmaCI	CACGTG	2 cut(s)	159, 753
PmlI	CACGTG	2 cut(s)	159, 753
PpsI	GAGTC	2 cut(s)	426, 1914
Ppu21I	YACGTR	2 cut(s)	159, 753
PpuMI	RGGWCCY	2 cut(s)	613, 1167
PsiI	TTATAA	2 cut(s)	1281, 1626
Psp124BI	GAGCTC	1 cut(s)	215
Psp5II	RGGWCCY	2 cut(s)	613, 1167
Psp6I	CCWGG	4 cut(s)	790, 1213, 1527, 1580
PspCI	CACGTG	2 cut(s)	159, 753
PspGI	CCWGG	4 cut(s)	790, 1213, 1527, 1580
PspN4I	GGNNCC	7 cut(s)	132, 615, 1141, 1168, 1169, 1223, 1632
PspPI	GGNCC	6 cut(s)	287, 305, 613, 1167, 1222, 1630
PspPPI	RGGWCCY	2 cut(s)	613, 1167
PsrI	GAACNNNNNNTAC	2 cut(s)	1540, 1572
PstNI	CAGNNNCTG	3 cut(s)	572, 1199, 1769
PsuI	RGATCY	2 cut(s)	248, 620
RsaI	GTAC	4 cut(s)	963, 1072, 1401, 1586
RsaNI	GTAC	4 cut(s)	962, 1071, 1400, 1585
RseI	CAYNNNNRTG	3 cut(s)	128, 161, 1362
SacI	GAGCTC	1 cut(s)	215
SaqAI	TTAA	6 cut(s)	887, 1061, 1434, 1451, 1791, 1913
SatI	GCNGC	6 cut(s)	83, 98, 1200, 1238, 1389, 1896
Sau3AI	GATC	5 cut(s)	248, 562, 620, 874, 1207
Sau96I	GGNCC	6 cut(s)	287, 305, 613, 1167, 1222, 1630
SchI	GAGTC	2 cut(s)	426, 1914
ScrFI	CCNGG	5 cut(s)	792, 1177, 1215, 1529, 1582
SduI	GDGCHC	1 cut(s)	215
SexAI	ACCWGGT	1 cut(s)	1527
SfaNI	GCATC	4 cut(s)	29, 202, 1415, 1579
SfcI	CTRYAG	3 cut(s)	687, 928, 981
Sfr274I	CTCGAG	1 cut(s)	693
SinI	GGWCC	3 cut(s)	305, 613, 1167
SlaI	CTCGAG	1 cut(s)	693
SmiMI	CAYNNNNRTG	3 cut(s)	128, 161, 1362
SmlI	CTYRAG	9 cut(s)	421, 693, 1018, 1231, 1293, 1501, 1800, 1912, 1924
SmoI	CTYRAG	9 cut(s)	421, 693, 1018, 1231, 1293, 1501, 1800, 1912, 1924
SpeI	ACTAGT	1 cut(s)	1834
SseBI	AGGCCT	1 cut(s)	1371
SsiI	CCGC	1 cut(s)	1917
SspMI	CTAG	6 cut(s)	86, 837, 1376, 1514, 1830, 1835
SstI	GAGCTC	1 cut(s)	215
StuI	AGGCCT	1 cut(s)	1371
StyD4I	CCNGG	5 cut(s)	790, 1175, 1213, 1527, 1580
StyI	CCWWGG	3 cut(s)	313, 493, 1881
TaiI	ACGT	4 cut(s)	161, 170, 755, 1941
TaqI	TCGA	5 cut(s)	149, 196, 694, 877, 899
TatI	WGTACW	1 cut(s)	1399
TfiI	GAWTC	5 cut(s)	177, 1009, 1289, 1718, 1775
Tru1I	TTAA	6 cut(s)	887, 1061, 1434, 1451, 1791, 1913
Tru9I	TTAA	6 cut(s)	887, 1061, 1434, 1451, 1791, 1913
TscAI	CASTG	4 cut(s)	385, 667, 1347, 1416
TseFI	GTSAC	4 cut(s)	163, 322, 656, 1097
TseI	GCWGC	6 cut(s)	82, 97, 1199, 1237, 1388, 1895
Tsp45I	GTSAC	4 cut(s)	163, 322, 656, 1097
TspDTI	ATGAA	6 cut(s)	49, 328, 570, 736, 761, 1671
TspGWI	ACGGA	2 cut(s)	61, 189
TspRI	CASTG	4 cut(s)	385, 667, 1347, 1416
Van91I	CCANNNNNTGG	1 cut(s)	314
Vha464I	CTTAAG	1 cut(s)	1912
VpaK11BI	GGWCC	3 cut(s)	305, 613, 1167
XagI	CCTNNNNNAGG	1 cut(s)	1625
XapI	RAATTY	4 cut(s)	385, 1063, 1414, 1825
XbaI	TCTAGA	1 cut(s)	1829
XceI	RCATGY	3 cut(s)	127, 1160, 1747
XhoI	CTCGAG	1 cut(s)	693
XmiI	GTMKAC	1 cut(s)	1678
XmnI	GAANNNNTTC	2 cut(s)	1008, 1418
XspI	CTAG	6 cut(s)	86, 837, 1376, 1514, 1830, 1835

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.