Rorug02G0337100

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008464
Physical Location & Seq
Forward (+)
41883471 .. 41884686
1216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0337100.1

Sequence Viewer

Length: 609 bp
ATGATGGAGATGGTGAATGTAAGCCAACGCAAGACGAAAAGGCAGAGCGAGAAGATGTCTCTGGAAGACTACCTCTTTCTCACTGAGTCTCGCTCCAACCTCCACCTCACCGTCACTCACCTCAATCAGATCATCAGCATGCACGGATTCAAAAAACTTCACAGACTTCACAAGAAAATTCTGAGCGACGCCGTGAGCACAATGGACCTGGTGGAACCGAGCCGCTCTACGCTCCGGGACTACATCTCGCCGCCGGTCAACACTTGTCTGAACGACGTCGTCGCGGACATGAACAACCTGAGCTGGCAGGAGTGCTGCGTCACCGCCATCAAAACGCTGAGCTCGTGTCAACTAAACGACGCCGTCGAGTGCTCGAAGAGCCACCAATCGCCTCCCACGGCTCTGGATTCCCACGGCGGCGGCGTTTCCGGTACTACATTTCAGGGAAGGGCTGAGCCGTTGAGGAAACTGGTGCAGTGGAAGAAGAGACGGACTCAGGAGAGCGCTGATCCGGTGGTGAAGAGGCAGAGGATGAGATCGGAAAGCGACGGTGGTGGTGGCGCCGCCGCTCTTGCTTGGGATTGTGTCTCGTTGAATTCTTTCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_6

Protein Analysis

202

Amino Acids

22.52

Weight (kDa)

8.94

Isoelectric Point (pI)

67.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7787 PF25042 16 - 71 1.1e-25 Domain of unknown function (DUF7787)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 279
AccB1I GGYRCC 1 cut(s) 560
AccBSI CCGCTC 2 cut(s) 225, 569
AccII CGCG 1 cut(s) 284
AciI CCGC 8 cut(s) 223, 251, 284, 324, 417, 420, 564, 567
AclWI GGATC 1 cut(s) 503
AcsI RAATTY 2 cut(s) 177, 595
AcyI GRCGYC 4 cut(s) 189, 276, 360, 561
AfaI GTAC 1 cut(s) 433
AfeI AGCGCT 1 cut(s) 505
AgsI TTSAA 2 cut(s) 151, 595
AjnI CCWGG 1 cut(s) 207
AluBI AGCT 2 cut(s) 303, 342
AluI AGCT 2 cut(s) 303, 342
Alw21I GWGCWC 3 cut(s) 200, 344, 374
Alw26I GTCTC 4 cut(s) 63, 93, 481, 592
AlwI GGATC 1 cut(s) 503
Aor51HI AGCGCT 1 cut(s) 505
ApeKI GCWGC 1 cut(s) 315
ApoI RAATTY 2 cut(s) 177, 595
Asp700I GAANNNNTTC 1 cut(s) 599
AspLEI GCGC 2 cut(s) 506, 563
AspS9I GGNCC 1 cut(s) 205
AsuC2I CCSGG 1 cut(s) 236
AsuHPI GGTGA 5 cut(s) 25, 100, 110, 313, 529
AvaII GGWCC 1 cut(s) 205
BanI GGYRCC 1 cut(s) 560
BanII GRGCYC 1 cut(s) 344
BauI CACGAG 1 cut(s) 343
BbsI GAAGAC 1 cut(s) 72
Bbv12I GWGCWC 3 cut(s) 200, 344, 374
BbvI GCAGC 1 cut(s) 302
BccI CCATC 2 cut(s) 4, 335
BceAI ACGGC 5 cut(s) 176, 347, 414, 430, 442
BciT130I CCWGG 1 cut(s) 209
BcnI CCSGG 1 cut(s) 236
BcoDI GTCTC 4 cut(s) 63, 93, 481, 592
BfoI RGCGCY 2 cut(s) 507, 564
BisI GCNGC 7 cut(s) 223, 251, 316, 418, 421, 564, 567
BlpI GCTNAGC 2 cut(s) 338, 453
BlsI GCNGC 7 cut(s) 224, 252, 317, 419, 422, 565, 568
Bme1390I CCNGG 2 cut(s) 209, 236
Bme18I GGWCC 1 cut(s) 205
BmgT120I GGNCC 1 cut(s) 205
BmiI GGNNCC 2 cut(s) 216, 562
BmrFI CCNGG 2 cut(s) 209, 236
BpiI GAAGAC 1 cut(s) 72
BplI GAGNNNNNCTC 4 cut(s) 77, 109, 478, 510
Bpu10I CCTNAGC 1 cut(s) 299
Bpu1102I GCTNAGC 2 cut(s) 338, 453
BpuMI CCSGG 1 cut(s) 236
BsaHI GRCGYC 4 cut(s) 189, 276, 360, 561
BsaJI CCNNGG 2 cut(s) 396, 412
BsaWI WCCGGW 2 cut(s) 428, 511
Bse118I RCCGGY 1 cut(s) 253
Bse1I ACTGG 1 cut(s) 474
BseBI CCWGG 1 cut(s) 209
BseDI CCNNGG 2 cut(s) 396, 412
BseGI GGATG 1 cut(s) 537
BseMII CTCAG 6 cut(s) 75, 173, 290, 329, 444, 509
BseNI ACTGG 1 cut(s) 474
BseXI GCAGC 1 cut(s) 302
BsgI GTGCAG 1 cut(s) 494
Bsh1236I CGCG 1 cut(s) 284
BshNI GGYRCC 1 cut(s) 560
BsiHKAI GWGCWC 3 cut(s) 200, 344, 374
BsiSI CCGG 4 cut(s) 235, 254, 429, 512
BslFI GGGAC 1 cut(s) 251
BsmAI GTCTC 4 cut(s) 63, 93, 481, 592
BsmBI CGTCTC 1 cut(s) 481
BsmFI GGGAC 1 cut(s) 251
Bsp1286I GDGCHC 3 cut(s) 200, 344, 374
Bsp143I GATC 3 cut(s) 129, 508, 536
Bsp1720I GCTNAGC 2 cut(s) 338, 453
BspACI CCGC 8 cut(s) 223, 251, 284, 324, 417, 420, 564, 567
BspCNI CTCAG 6 cut(s) 76, 174, 291, 330, 445, 508
BspFNI CGCG 1 cut(s) 284
BspLI GGNNCC 2 cut(s) 216, 562
BspPI GGATC 1 cut(s) 503
BspQI GCTCTTC 1 cut(s) 371
BspT107I GGYRCC 1 cut(s) 560
BsrBI CCGCTC 2 cut(s) 225, 569
BsrFI RCCGGY 1 cut(s) 253
BsrI ACTGG 1 cut(s) 474
BssAI RCCGGY 1 cut(s) 253
BssECI CCNNGG 2 cut(s) 396, 412
BssMI GATC 3 cut(s) 129, 508, 536
BssNI GRCGYC 4 cut(s) 189, 276, 360, 561
BssSI CACGAG 1 cut(s) 343
Bst2BI CACGAG 1 cut(s) 343
Bst2UI CCWGG 1 cut(s) 209
Bst4CI ACNGT 2 cut(s) 112, 551
Bst6I CTCTTC 3 cut(s) 371, 479, 515
BstACI GRCGYC 4 cut(s) 189, 276, 360, 561
BstC8I GCNNGC 2 cut(s) 140, 305
BstDEI CTNAG 6 cut(s) 84, 182, 299, 338, 453, 495
BstDSI CCRYGG 2 cut(s) 396, 412
BstF5I GGATG 1 cut(s) 537
BstFNI CGCG 1 cut(s) 284
BstH2I RGCGCY 2 cut(s) 507, 564
BstHHI GCGC 2 cut(s) 506, 563
BstKTI GATC 3 cut(s) 132, 511, 539
BstMAI GTCTC 4 cut(s) 63, 93, 481, 592
BstMBI GATC 3 cut(s) 129, 508, 536
BstMWI GCNNNNNNNGC 2 cut(s) 378, 572
BstNI CCWGG 1 cut(s) 209
BstNSI RCATGY 1 cut(s) 142
BstSCI CCNGG 2 cut(s) 207, 234
BstUI CGCG 1 cut(s) 284
BstV1I GCAGC 1 cut(s) 302
BstV2I GAAGAC 1 cut(s) 72
BstXI CCANNNNNNTGG 1 cut(s) 403
BtgI CCRYGG 2 cut(s) 396, 412
BtsCI GGATG 1 cut(s) 537
BtsI GCAGTG 1 cut(s) 482
BtsIMutI CAGTG 2 cut(s) 81, 482
Cac8I GCNNGC 2 cut(s) 140, 305
CfoI GCGC 2 cut(s) 506, 563
Cfr10I RCCGGY 1 cut(s) 253
Cfr13I GGNCC 1 cut(s) 205
CseI GACGC 3 cut(s) 197, 307, 368
CsiI ACCWGGT 1 cut(s) 207
Csp6I GTAC 1 cut(s) 432
CviAII CATG 2 cut(s) 139, 289
CviJI RGCY 8 cut(s) 24, 222, 303, 342, 381, 401, 452, 457
CviKI_1 RGCY 8 cut(s) 24, 222, 303, 342, 381, 401, 452, 457
CviQI GTAC 1 cut(s) 432
DdeI CTNAG 6 cut(s) 84, 182, 299, 338, 453, 495
DinI GGCGCC 1 cut(s) 562
DpnI GATC 3 cut(s) 131, 510, 538
DpnII GATC 3 cut(s) 129, 508, 536
Eam1104I CTCTTC 3 cut(s) 371, 479, 515
EarI CTCTTC 3 cut(s) 371, 479, 515
Ecl136II GAGCTC 1 cut(s) 342
Eco24I GRGCYC 1 cut(s) 344
Eco47I GGWCC 1 cut(s) 205
Eco47III AGCGCT 1 cut(s) 505
Eco53kI GAGCTC 1 cut(s) 342
EcoICRI GAGCTC 1 cut(s) 342
EcoRI GAATTC 1 cut(s) 595
EcoRII CCWGG 1 cut(s) 207
EcoT38I GRGCYC 1 cut(s) 344
EgeI GGCGCC 1 cut(s) 562
EheI GGCGCC 1 cut(s) 562
Esp3I CGTCTC 1 cut(s) 481
FaeI CATG 2 cut(s) 142, 292
FaiI YATR 2 cut(s) 140, 290
FaqI GGGAC 1 cut(s) 251
FatI CATG 2 cut(s) 138, 288
Fnu4HI GCNGC 7 cut(s) 223, 251, 316, 418, 421, 564, 567
FokI GGATG 1 cut(s) 544
FriOI GRGCYC 1 cut(s) 344
Fsp4HI GCNGC 7 cut(s) 223, 251, 316, 418, 421, 564, 567
GlaI GCGC 2 cut(s) 505, 562
GluI GCNGC 7 cut(s) 223, 251, 316, 418, 421, 564, 567
HaeII RGCGCY 2 cut(s) 507, 564
HapII CCGG 4 cut(s) 235, 254, 429, 512
HgaI GACGC 3 cut(s) 197, 307, 368
HhaI GCGC 2 cut(s) 506, 563
Hin1I GRCGYC 4 cut(s) 189, 276, 360, 561
Hin1II CATG 2 cut(s) 142, 292
Hin6I GCGC 2 cut(s) 504, 561
HinP1I GCGC 2 cut(s) 504, 561
HincII GTYRAC 2 cut(s) 259, 350
HindII GTYRAC 2 cut(s) 259, 350
HinfI GANTC 4 cut(s) 86, 147, 407, 493
HpaII CCGG 4 cut(s) 235, 254, 429, 512
HphI GGTGA 5 cut(s) 25, 100, 110, 313, 529
Hpy166II GTNNAC 2 cut(s) 259, 350
Hpy188I TCNGA 4 cut(s) 129, 183, 270, 541
Hpy188III TCNNGA 3 cut(s) 62, 404, 497
Hpy8I GTNNAC 2 cut(s) 259, 350
Hpy99I CGWCG 7 cut(s) 191, 278, 281, 284, 362, 368, 551
HpyAV CCTTC 1 cut(s) 441
HpyCH4III ACNGT 2 cut(s) 112, 551
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 2 cut(s) 142, 475
HpyF10VI GCNNNNNNNGC 2 cut(s) 378, 572
HpyF3I CTNAG 6 cut(s) 84, 182, 299, 338, 453, 495
HpySE526I ACGT 1 cut(s) 276
Hsp92I GRCGYC 4 cut(s) 189, 276, 360, 561
Hsp92II CATG 2 cut(s) 142, 292
HspAI GCGC 2 cut(s) 504, 561
KasI GGCGCC 1 cut(s) 560
Kzo9I GATC 3 cut(s) 129, 508, 536
LguI GCTCTTC 1 cut(s) 371
LmnI GCTCC 2 cut(s) 98, 237
Lsp1109I GCAGC 1 cut(s) 302
MabI ACCWGGT 1 cut(s) 207
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 2 cut(s) 112, 319
MalI GATC 3 cut(s) 131, 510, 538
MbiI CCGCTC 2 cut(s) 225, 569
MboI GATC 3 cut(s) 129, 508, 536
MboII GAAGA 6 cut(s) 64, 77, 388, 493, 496, 532
MhlI GDGCHC 3 cut(s) 200, 344, 374
MluCI AATT 2 cut(s) 177, 595
Mly113I GGCGCC 1 cut(s) 561
MlyI GAGTC 2 cut(s) 95, 487
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 8 cut(s) 83, 110, 116, 131, 402, 456, 516, 522
MroXI GAANNNNTTC 1 cut(s) 599
MslI CAYNNNNRTG 1 cut(s) 137
MspI CCGG 4 cut(s) 235, 254, 429, 512
MspR9I CCNGG 2 cut(s) 209, 236
MvaI CCWGG 1 cut(s) 209
MvnI CGCG 1 cut(s) 284
MwoI GCNNNNNNNGC 2 cut(s) 378, 572
NarI GGCGCC 1 cut(s) 561
NciI CCSGG 1 cut(s) 236
NdeII GATC 3 cut(s) 129, 508, 536
NlaIII CATG 2 cut(s) 142, 292
NlaIV GGNNCC 2 cut(s) 216, 562
NmuCI GTSAC 2 cut(s) 112, 319
NspI RCATGY 1 cut(s) 142
PaeI GCATGC 1 cut(s) 142
PciSI GCTCTTC 1 cut(s) 371
PcsI WCGNNNNNNNCGW 3 cut(s) 341, 363, 420
PdmI GAANNNNTTC 1 cut(s) 599
PfeI GAWTC 2 cut(s) 147, 407
PflFI GACNNNGTC 2 cut(s) 278, 362
PfoI TCCNGGA 1 cut(s) 234
PkrI GCNGC 7 cut(s) 224, 252, 317, 419, 422, 565, 568
PleI GAGTC 2 cut(s) 94, 487
PluTI GGCGCC 1 cut(s) 564
PpsI GAGTC 2 cut(s) 94, 487
Psp124BI GAGCTC 1 cut(s) 344
Psp6I CCWGG 1 cut(s) 207
PspGI CCWGG 1 cut(s) 207
PspN4I GGNNCC 2 cut(s) 216, 562
PspPI GGNCC 1 cut(s) 205
PsyI GACNNNGTC 2 cut(s) 278, 362
RsaI GTAC 1 cut(s) 433
RsaNI GTAC 1 cut(s) 432
RseI CAYNNNNRTG 1 cut(s) 137
SacI GAGCTC 1 cut(s) 344
SapI GCTCTTC 1 cut(s) 371
SatI GCNGC 7 cut(s) 223, 251, 316, 418, 421, 564, 567
Sau3AI GATC 3 cut(s) 129, 508, 536
Sau96I GGNCC 1 cut(s) 205
SchI GAGTC 2 cut(s) 95, 487
ScrFI CCNGG 2 cut(s) 209, 236
SduI GDGCHC 3 cut(s) 200, 344, 374
SetI ASST 9 cut(s) 75, 102, 108, 123, 210, 279, 300, 305, 344
SexAI ACCWGGT 1 cut(s) 207
SfoI GGCGCC 1 cut(s) 562
SinI GGWCC 1 cut(s) 205
SmiMI CAYNNNNRTG 1 cut(s) 137
SphI GCATGC 1 cut(s) 142
Sse9I AATT 2 cut(s) 177, 595
SsiI CCGC 8 cut(s) 223, 251, 284, 324, 417, 420, 564, 567
SspDI GGCGCC 1 cut(s) 560
SstI GAGCTC 1 cut(s) 344
StyD4I CCNGG 2 cut(s) 207, 234
TaaI ACNGT 2 cut(s) 112, 551
TaiI ACGT 1 cut(s) 279
TaqI TCGA 2 cut(s) 366, 374
TasI AATT 2 cut(s) 177, 595
TauI GCSGC 6 cut(s) 225, 253, 420, 423, 566, 569
TfiI GAWTC 2 cut(s) 147, 407
TscAI CASTG 2 cut(s) 88, 482
TseFI GTSAC 2 cut(s) 112, 319
TseI GCWGC 1 cut(s) 315
Tsp45I GTSAC 2 cut(s) 112, 319
TspDTI ATGAA 1 cut(s) 305
TspGWI ACGGA 2 cut(s) 159, 505
TspRI CASTG 2 cut(s) 88, 482
Tth111I GACNNNGTC 2 cut(s) 278, 362
VpaK11BI GGWCC 1 cut(s) 205
XapI RAATTY 2 cut(s) 177, 595
XceI RCATGY 1 cut(s) 142
XmnI GAANNNNTTC 1 cut(s) 599
ZraI GACGTC 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.