Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0004927

Physical Location & Seq

Genomic Coordinates Forward (+)

22394117 .. 22398311

Gene Structure 4195 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1219100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2022 bp

ATGGAGAGCTTCAAGTCCCCATTCAGGGGTATTGCAAATGATGTCCGAGGAAGAGCAGCATGCTATAAACACGATTGGGTTGGAGGTTTTCAGGCAGGAATCGGGATTCTCGCCCCAACAACCTACATCTTCTTCGCTTCAGCTCTTCCTGTTATAGCATTTGGAGAGCAACTGAGCACAGATACAGATGGAATTTTGAGCACGGTAGAAACCTTGGCCTCTACTGCTATTTGTGGTATAATACACTCGATTTTTGGTGGACAACCTCTCTTGATTTTAGGAGTCGCCGAACCCACTGTTATTATGTACAATTACATGTACGACTTTGCCAAAGGAAGGAAAGAATTGGGGCCGGAGCTGTTTTTGGCTTGGGTTGCATGGGTTTGGGTCTGGACAGCTCTTTTCCTCATTCTTCTTGCACTATTCAATGCTTGTGATATCATCAACAGATTTACGAGGATTGCAGGCGAACTTTTTGGCATGTTGATTTCTGTTTTATTCATTCAAGAGGCTATCAAGGGTATAGTGAGTGAATTCAAAATTCCCAAAGGCGAAGATCCAAAGTTGGAGACGTATCAATTTCAGTGGCTTTACACAAATGGACTGTTAGGGGTCATATTTACTTTTGGACTACTGTATACTGCTTTGAAGAGCAGAAAGGCAAGGTCCTGGTGGTATGCAACAGGATGGTTCAGAAGTTTCATTGCAGATTATGGAGTTCCTTTAATGGTGTTGGTATGGACTGCATTATCATTTAGTGTCCCACACAATGTCCCTCCAAGAGTTCCCAGAAGGCTCTATATCCCTGTAGCTTGGGATTCTGCGTCTTTACACCATTGGACTGTCATCAAGGATATGGGGAAGGTTTCTCCTGGATACGTCTTTGCTGCCATTATACCTGCTGTGATGGTGGCTGGACTTTATTTTTTTGACCATAGTGTCGCTTCACAGATGGCACAACAACATGAGTTCAATCTAAAGAAGCCGCCTGCGTATCATTATGATATCTTGTTGTTGGGATTCATGACTCTGCTTTGCGGATTGCTTGGGCTTCCTCCTTCTAACGGGGTTCTGCCACAGTCACCGATGCACACTAAGAGCCTTGCTGTTCTTAAGAAGCAGTCGATACGAAGGAAAATGGTGAAGAGTGCTAAGGAATCAATAAAACAGAAAGCTAACAACTCTGAAATCTATGGGAAAATGCAAGCTGTGTTCATAGAGATGGACAACTTGACTACACCTACTTCGGAAGTTAAAGAGCTAAATGATTTGAAGGAGGCAGTTATGAAGAGTGAAAATAAAGGGGAGGATACAACGGATGCATTTGATCCTGAGAAGCACATTGATGCATACTTGCCAGTCAGAGTTACTGAGCAAAGATTGAGCAATTTGTTGCAGTCGCTTCTTGTGGCAGCATCAGTTTTTGCCATGCCTGCCATCAAGAAGATACCGACATCAGTTTTGTGGGGATATTTTGCTTACATGGCCATTGACAGCCTTCCAGGAAACCAATTCTGGGAAAGGATGCTACTACTCTTCATCTCCCCTAGCCGACAGTACAAGGTCTTGGAAGGGGGCCATGCTTCCTTTGTGGAGTCAGTGCCATTCAAATTCATAGCCATATTTACACTCTTCCAGATTGTTTACTTGCTGGTCTGTTTTGGTATTACATGGATCCCCATGGCTGGAATTTTGTTCCCCTTGCCTTTCTTCCTTCTCATATGGATAAGACAGCATCTCCTCCCCAAATTCTTCCAATCACATCACTTGCAAGAGCTGGACTCAGCTGAATGGGAGGAAGTTGTGGGCGCCCCAGTACGCTCTCTCAGCCTCAGTTCTGGGAAATCGGAAACTTCCAACGAAGGTGGAGTGGAGATGTGTGATGCTGAGATATTAGACAAGCTAACAACCAGCAGAGGGGAGCTGAAGATCAGAGCGAGCTTCAGCGAAGACAAACGAACCAGCTTCAGTGAAGATAGACGCGGCCAGGTTCACCCTCAGAATTTAGGCCAAACAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006820 GO:0006855 GO:0006873 GO:0006885 GO:0007154 GO:0008150 GO:0008509 GO:0009605 GO:0009987 GO:0009991 GO:0010036 GO:0015075 GO:0015103 GO:0015238 GO:0015318 GO:0015562 GO:0015698 GO:0015893 GO:0016020 GO:0016021 GO:0019725 GO:0022857 GO:0030003 GO:0030004 GO:0030641 GO:0031224 GO:0031226 GO:0031667 GO:0031668 GO:0031669 GO:0034220 GO:0035445 GO:0042221 GO:0042493 GO:0042592 GO:0044425 GO:0044459 GO:0044464 GO:0046713 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051453 GO:0051716 GO:0055067 GO:0055080 GO:0055082 GO:0055085 GO:0065007 GO:0065008 GO:0070887 GO:0071496 GO:0071944 GO:0080029 GO:0080139 GO:0098656 GO:0098660 GO:0098661 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HCO3_cotransp

Protein Analysis

674

Amino Acids

75.6

Weight (kDa)

7.66

Isoelectric Point (pI)

44.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HCO3_cotransp	PF00955	7 - 181	5.4e-38	HCO3- transporter integral membrane domain
HCO3_cotransp	PF00955	201 - 375	5.2e-25	HCO3- transporter integral membrane domain
HCO3_cotransp	PF00955	454 - 596	1.1e-30	HCO3- transporter integral membrane domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004927)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15460 AT1G15460 AT1G15460 AT1G15460 AT1G74810 AT1G74810 AT1G74810 AT1G74810 AT1G74810 AT1G74810 AT1G74810
fragaria_vesca	FvH4_6g30530 FvH4_6g30530
malus_domestica	MD09G1219100.v1.1 MD17G1202200.v1.1
prunus_persica	Prupe.3G077900_v2.0.a1 Prupe.3G077900_v2.0.a1
pyrus_communis	pycom17g20650
rosa_chinensis	RchiOBHm_Chr2g0136951
rosa_laevigata	RLG00000019590
rosa_multiflora	Rmu_sc0001374.1_g000070
rosa_roxburghii	Rroxscaffold_2G00107860
rosa_rugosa	Rorug02G0335500 Rorug02G0335500 Rorug02G0335500 Rorug02G0335600
rosa_samantha	Rh2AG385500 Rh2BG392500 Rh2CG372700
rosa_wichuraiana	Rw2G031530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	938
Acc36I	ACCTGC	1 cut(s)	907
AccB1I	GGYRCC	1 cut(s)	1808
AccI	GTMKAC	1 cut(s)	638
AccII	CGCG	1 cut(s)	1983
AciI	CCGC	3 cut(s)	986, 1038, 1983
AclWI	GGATC	4 cut(s)	551, 1322, 1669, 1682
AcoI	YGGCCR	2 cut(s)	1485, 1984
AcsI	RAATTY	7 cut(s)	192, 533, 540, 1610, 1689, 1748, 2002
AcuI	CTGAAG	4 cut(s)	123, 1927, 1946, 1951
AcyI	GRCGYC	1 cut(s)	1809
AfaI	GTAC	4 cut(s)	308, 320, 1559, 1818
AfiI	CCNNNNNNNGG	8 cut(s)	24, 25, 26, 336, 1064, 1516, 1685, 1917
AflII	CTTAAG	1 cut(s)	1112
AflIII	ACRYGT	1 cut(s)	315
AgsI	TTSAA	8 cut(s)	13, 427, 506, 538, 649, 973, 1273, 1609
AjnI	CCWGG	4 cut(s)	668, 871, 1501, 1986
Alw21I	GWGCWC	2 cut(s)	179, 203
Alw26I	GTCTC	1 cut(s)	563
AlwI	GGATC	4 cut(s)	551, 1322, 1669, 1682
AoxI	GGCC	6 cut(s)	216, 350, 1485, 1576, 1984, 2008
ApeKI	GCWGC	3 cut(s)	56, 887, 1412
ApoI	RAATTY	7 cut(s)	192, 533, 540, 1610, 1689, 1748, 2002
ArsI	GACNNNNNNTTYG	2 cut(s)	1444, 1476
AspLEI	GCGC	1 cut(s)	1811
AspS9I	GGNCC	3 cut(s)	350, 666, 1576
AsuHPI	GGTGA	3 cut(s)	1074, 1153, 1985
AvaII	GGWCC	1 cut(s)	666
BalI	TGGCCA	1 cut(s)	1487
BamHI	GGATCC	1 cut(s)	1674
BanI	GGYRCC	1 cut(s)	1808
BbsI	GAAGAC	1 cut(s)	1956
Bbv12I	GWGCWC	2 cut(s)	179, 203
BbvI	GCAGC	3 cut(s)	68, 874, 1424
BccI	CCATC	6 cut(s)	182, 681, 901, 946, 1216, 1445
BciT130I	CCWGG	4 cut(s)	670, 873, 1503, 1988
BciVI	GTATCC	2 cut(s)	869, 1303
BcoDI	GTCTC	1 cut(s)	563
BfaI	CTAG	1 cut(s)	1548
BfmI	CTRYAG	1 cut(s)	807
BfoI	RGCGCY	1 cut(s)	1812
BfrI	CTTAAG	1 cut(s)	1112
BfuAI	ACCTGC	1 cut(s)	907
BfuI	GTATCC	2 cut(s)	869, 1303
BisI	GCNGC	5 cut(s)	57, 888, 986, 1413, 1984
BlsI	GCNGC	5 cut(s)	58, 889, 987, 1414, 1985
Bme1390I	CCNGG	4 cut(s)	670, 873, 1503, 1988
Bme18I	GGWCC	1 cut(s)	666
BmgT120I	GGNCC	3 cut(s)	350, 666, 1576
BmiI	GGNNCC	4 cut(s)	351, 1577, 1676, 1810
BmrFI	CCNGG	4 cut(s)	670, 873, 1503, 1988
BmrI	ACTGGG	1 cut(s)	1808
BmsI	GCATC	7 cut(s)	1077, 1309, 1336, 1424, 1515, 1744, 1873
BmuI	ACTGGG	1 cut(s)	1808
BpiI	GAAGAC	1 cut(s)	1956
BplI	GAGNNNNNCTC	2 cut(s)	1766, 1798
Bpu10I	CCTNAGC	1 cut(s)	1152
BsaHI	GRCGYC	1 cut(s)	1809
BsaJI	CCNNGG	3 cut(s)	46, 213, 1680
BsaXI	ACNNNNNCTCC	2 cut(s)	1722, 1752
Bsc4I	CCNNNNNNNGG	8 cut(s)	24, 25, 26, 336, 1064, 1516, 1685, 1917
Bse1I	ACTGG	2 cut(s)	1358, 1814
Bse3DI	GCAATG	1 cut(s)	702
BseBI	CCWGG	4 cut(s)	670, 873, 1503, 1988
BseDI	CCNNGG	3 cut(s)	46, 213, 1680
BseGI	GGATG	3 cut(s)	692, 1324, 1530
BseLI	CCNNNNNNNGG	8 cut(s)	24, 25, 26, 336, 1064, 1516, 1685, 1917
BseMI	GCAATG	1 cut(s)	702
BseMII	CTCAG	8 cut(s)	164, 1323, 1362, 1797, 1840, 1846, 1878, 2012
BseNI	ACTGG	2 cut(s)	1358, 1814
BseRI	GAGGAG	1 cut(s)	1730
BseXI	GCAGC	3 cut(s)	68, 874, 1424
Bsh1236I	CGCG	1 cut(s)	1983
BshFI	GGCC	6 cut(s)	218, 352, 1487, 1578, 1986, 2010
BshNI	GGYRCC	1 cut(s)	1808
BsiHKAI	GWGCWC	2 cut(s)	179, 203
BsiSI	CCGG	1 cut(s)	353
BslFI	GGGAC	2 cut(s)	746, 758
BslI	CCNNNNNNNGG	8 cut(s)	24, 25, 26, 336, 1064, 1516, 1685, 1917
BsmAI	GTCTC	1 cut(s)	563
BsmBI	CGTCTC	1 cut(s)	563
BsmFI	GGGAC	2 cut(s)	746, 758
BsnI	GGCC	6 cut(s)	218, 352, 1487, 1578, 1986, 2010
Bsp1286I	GDGCHC	2 cut(s)	179, 203
Bsp1407I	TGTACA	1 cut(s)	306
Bsp143I	GATC	4 cut(s)	556, 1327, 1674, 1929
Bsp19I	CCATGG	1 cut(s)	1680
BspACI	CCGC	3 cut(s)	986, 1038, 1983
BspANI	GGCC	6 cut(s)	218, 352, 1487, 1578, 1986, 2010
BspCNI	CTCAG	8 cut(s)	165, 1324, 1363, 1796, 1839, 1845, 1879, 2011
BspFNI	CGCG	1 cut(s)	1983
BspHI	TCATGA	1 cut(s)	1023
BspLI	GGNNCC	4 cut(s)	351, 1577, 1676, 1810
BspMI	ACCTGC	1 cut(s)	907
BspPI	GGATC	4 cut(s)	551, 1322, 1669, 1682
BspQI	GCTCTTC	3 cut(s)	46, 150, 644
BspT107I	GGYRCC	1 cut(s)	1808
BspTI	CTTAAG	1 cut(s)	1112
BsrDI	GCAATG	1 cut(s)	702
BsrGI	TGTACA	1 cut(s)	306
BsrI	ACTGG	2 cut(s)	1358, 1814
BssECI	CCNNGG	3 cut(s)	46, 213, 1680
BssMI	GATC	4 cut(s)	556, 1327, 1674, 1929
BssNAI	GTATAC	1 cut(s)	639
BssNI	GRCGYC	1 cut(s)	1809
BssT1I	CCWWGG	2 cut(s)	213, 1680
Bst1107I	GTATAC	1 cut(s)	639
Bst2UI	CCWGG	4 cut(s)	670, 873, 1503, 1988
Bst4CI	ACNGT	7 cut(s)	205, 298, 606, 636, 844, 1080, 1557
Bst6I	CTCTTC	7 cut(s)	46, 150, 644, 1139, 1283, 1541, 1637
BstACI	GRCGYC	1 cut(s)	1809
BstAFI	CTTAAG	1 cut(s)	1112
BstAUI	TGTACA	1 cut(s)	306
BstC8I	GCNNGC	6 cut(s)	61, 466, 990, 1206, 1434, 1939
BstDSI	CCRYGG	1 cut(s)	1680
BstF5I	GGATG	3 cut(s)	692, 1324, 1530
BstFNI	CGCG	1 cut(s)	1983
BstH2I	RGCGCY	1 cut(s)	1812
BstHHI	GCGC	1 cut(s)	1811
BstKTI	GATC	4 cut(s)	559, 1330, 1677, 1932
BstMAI	GTCTC	1 cut(s)	563
BstMBI	GATC	4 cut(s)	556, 1327, 1674, 1929
BstMWI	GCNNNNNNNGC	5 cut(s)	224, 374, 1433, 1484, 1827
BstNI	CCWGG	4 cut(s)	670, 873, 1503, 1988
BstNSI	RCATGY	3 cut(s)	63, 319, 484
BstSCI	CCNGG	4 cut(s)	668, 871, 1501, 1986
BstSFI	CTRYAG	1 cut(s)	807
BstUI	CGCG	1 cut(s)	1983
BstV1I	GCAGC	3 cut(s)	68, 874, 1424
BstV2I	GAAGAC	1 cut(s)	1956
BstX2I	RGATCY	2 cut(s)	556, 1674
BstYI	RGATCY	2 cut(s)	556, 1674
BstZ17I	GTATAC	1 cut(s)	639
BsuI	GTATCC	2 cut(s)	869, 1303
BsuRI	GGCC	6 cut(s)	218, 352, 1487, 1578, 1986, 2010
BtgI	CCRYGG	1 cut(s)	1680
BtsCI	GGATG	3 cut(s)	692, 1324, 1530
BtsIMutI	CAGTG	4 cut(s)	294, 590, 1605, 1975
BveI	ACCTGC	1 cut(s)	907
Cac8I	GCNNGC	6 cut(s)	61, 466, 990, 1206, 1434, 1939
CciI	TCATGA	1 cut(s)	1023
CfoI	GCGC	1 cut(s)	1811
Cfr13I	GGNCC	3 cut(s)	350, 666, 1576
CseI	GACGC	2 cut(s)	813, 1989
Csp6I	GTAC	4 cut(s)	307, 319, 1558, 1817
CspCI	CAANNNNNGTGG	4 cut(s)	566, 601, 1846, 1881
CviQI	GTAC	4 cut(s)	307, 319, 1558, 1817
DinI	GGCGCC	1 cut(s)	1810
DpnI	GATC	4 cut(s)	558, 1329, 1676, 1931
DpnII	GATC	4 cut(s)	556, 1327, 1674, 1929
DrdI	GACNNNNNNGTC	1 cut(s)	938
DseDI	GACNNNNNNGTC	1 cut(s)	938
EaeI	YGGCCR	2 cut(s)	1485, 1984
Eam1104I	CTCTTC	7 cut(s)	46, 150, 644, 1139, 1283, 1541, 1637
EarI	CTCTTC	7 cut(s)	46, 150, 644, 1139, 1283, 1541, 1637
Eco130I	CCWWGG	2 cut(s)	213, 1680
Eco32I	GATATC	2 cut(s)	439, 1006
Eco47I	GGWCC	1 cut(s)	666
Eco57I	CTGAAG	4 cut(s)	123, 1927, 1946, 1951
EcoO109I	RGGNCCY	1 cut(s)	666
EcoRI	GAATTC	1 cut(s)	533
EcoRII	CCWGG	4 cut(s)	668, 871, 1501, 1986
EcoRV	GATATC	2 cut(s)	439, 1006
EcoT14I	CCWWGG	2 cut(s)	213, 1680
EcoT22I	ATGCAT	2 cut(s)	1324, 1351
EgeI	GGCGCC	1 cut(s)	1810
EheI	GGCGCC	1 cut(s)	1810
ErhI	CCWWGG	2 cut(s)	213, 1680
Esp3I	CGTCTC	1 cut(s)	563
FaqI	GGGAC	2 cut(s)	746, 758
FauNDI	CATATG	1 cut(s)	1721
FblI	GTMKAC	1 cut(s)	638
Fnu4HI	GCNGC	5 cut(s)	57, 888, 986, 1413, 1984
FokI	GGATG	3 cut(s)	699, 1331, 1537
Fsp4HI	GCNGC	5 cut(s)	57, 888, 986, 1413, 1984
FspBI	CTAG	1 cut(s)	1548
GlaI	GCGC	1 cut(s)	1810
GluI	GCNGC	5 cut(s)	57, 888, 986, 1413, 1984
HaeII	RGCGCY	1 cut(s)	1812
HaeIII	GGCC	6 cut(s)	218, 352, 1487, 1578, 1986, 2010
HapII	CCGG	1 cut(s)	353
HgaI	GACGC	2 cut(s)	813, 1989
HhaI	GCGC	1 cut(s)	1811
Hin1I	GRCGYC	1 cut(s)	1809
Hin6I	GCGC	1 cut(s)	1809
HinP1I	GCGC	1 cut(s)	1809
HinfI	GANTC	9 cut(s)	99, 106, 282, 818, 1020, 1027, 1157, 1595, 1781
HpaII	CCGG	1 cut(s)	353
HphI	GGTGA	3 cut(s)	1074, 1153, 1985
Hpy166II	GTNNAC	4 cut(s)	260, 639, 1645, 1993
Hpy188I	TCNGA	8 cut(s)	47, 695, 1186, 1249, 1364, 1849, 1934, 2001
Hpy188III	TCNNGA	8 cut(s)	103, 271, 391, 506, 1024, 1331, 1441, 1636
Hpy8I	GTNNAC	4 cut(s)	260, 639, 1645, 1993
HpyCH4III	ACNGT	7 cut(s)	205, 298, 606, 636, 844, 1080, 1557
HpyCH4IV	ACGT	2 cut(s)	572, 879
HpyF10VI	GCNNNNNNNGC	5 cut(s)	224, 374, 1433, 1484, 1827
HpySE526I	ACGT	2 cut(s)	572, 879
Hsp92I	GRCGYC	1 cut(s)	1809
HspAI	GCGC	1 cut(s)	1809
KasI	GGCGCC	1 cut(s)	1808
Kzo9I	GATC	4 cut(s)	556, 1327, 1674, 1929
LguI	GCTCTTC	3 cut(s)	46, 150, 644
LmnI	GCTCC	2 cut(s)	355, 1921
Lsp1109I	GCAGC	3 cut(s)	68, 874, 1424
LweI	GCATC	7 cut(s)	1077, 1309, 1336, 1424, 1515, 1744, 1873
MaeI	CTAG	1 cut(s)	1548
MaeII	ACGT	2 cut(s)	572, 879
MaeIII	GTNAC	2 cut(s)	1080, 1366
MalI	GATC	4 cut(s)	558, 1329, 1676, 1931
MboI	GATC	4 cut(s)	556, 1327, 1674, 1929
MflI	RGATCY	2 cut(s)	556, 1674
MhlI	GDGCHC	2 cut(s)	179, 203
MlsI	TGGCCA	1 cut(s)	1487
MluNI	TGGCCA	1 cut(s)	1487
Mly113I	GGCGCC	1 cut(s)	1809
MlyI	GAGTC	4 cut(s)	291, 1021, 1604, 1775
MmeI	TCCRAC	3 cut(s)	61, 546, 1881
Mox20I	TGGCCA	1 cut(s)	1487
Mph1103I	ATGCAT	2 cut(s)	1324, 1351
MscI	TGGCCA	1 cut(s)	1487
MseI	TTAA	3 cut(s)	725, 1113, 1254
MslI	CAYNNNNRTG	2 cut(s)	1220, 1344
Msp20I	TGGCCA	1 cut(s)	1487
MspA1I	CMGCKG	1 cut(s)	1787
MspCI	CTTAAG	1 cut(s)	1112
MspI	CCGG	1 cut(s)	353
MspR9I	CCNGG	4 cut(s)	670, 873, 1503, 1988
MvaI	CCWGG	4 cut(s)	670, 873, 1503, 1988
MvnI	CGCG	1 cut(s)	1983
MwoI	GCNNNNNNNGC	5 cut(s)	224, 374, 1433, 1484, 1827
NarI	GGCGCC	1 cut(s)	1809
NcoI	CCATGG	1 cut(s)	1680
NdeI	CATATG	1 cut(s)	1721
NdeII	GATC	4 cut(s)	556, 1327, 1674, 1929
NlaIV	GGNNCC	4 cut(s)	351, 1577, 1676, 1810
NmuCI	GTSAC	1 cut(s)	1080
NsiI	ATGCAT	2 cut(s)	1324, 1351
NspI	RCATGY	3 cut(s)	63, 319, 484
PaeI	GCATGC	1 cut(s)	63
PagI	TCATGA	1 cut(s)	1023
PciI	ACATGT	1 cut(s)	315
PciSI	GCTCTTC	3 cut(s)	46, 150, 644
PfeI	GAWTC	5 cut(s)	99, 106, 818, 1020, 1157
PfoI	TCCNGGA	2 cut(s)	871, 1501
PkrI	GCNGC	5 cut(s)	58, 889, 987, 1414, 1985
PleI	GAGTC	4 cut(s)	290, 1021, 1603, 1775
PluTI	GGCGCC	1 cut(s)	1812
PpsI	GAGTC	4 cut(s)	290, 1021, 1603, 1775
PpuMI	RGGWCCY	1 cut(s)	666
PscI	ACATGT	1 cut(s)	315
Psp5II	RGGWCCY	1 cut(s)	666
Psp6I	CCWGG	4 cut(s)	668, 871, 1501, 1986
PspGI	CCWGG	4 cut(s)	668, 871, 1501, 1986
PspN4I	GGNNCC	4 cut(s)	351, 1577, 1676, 1810
PspPI	GGNCC	3 cut(s)	350, 666, 1576
PspPPI	RGGWCCY	1 cut(s)	666
PsuI	RGATCY	2 cut(s)	556, 1674
PvuII	CAGCTG	1 cut(s)	1787
RsaI	GTAC	4 cut(s)	308, 320, 1559, 1818
RsaNI	GTAC	4 cut(s)	307, 319, 1558, 1817
RseI	CAYNNNNRTG	2 cut(s)	1220, 1344
SapI	GCTCTTC	3 cut(s)	46, 150, 644
SaqAI	TTAA	3 cut(s)	725, 1113, 1254
SatI	GCNGC	5 cut(s)	57, 888, 986, 1413, 1984
Sau3AI	GATC	4 cut(s)	556, 1327, 1674, 1929
Sau96I	GGNCC	3 cut(s)	350, 666, 1576
SchI	GAGTC	4 cut(s)	291, 1021, 1604, 1775
ScrFI	CCNGG	4 cut(s)	670, 873, 1503, 1988
SduI	GDGCHC	2 cut(s)	179, 203
SfaNI	GCATC	7 cut(s)	1077, 1309, 1336, 1424, 1515, 1744, 1873
SfcI	CTRYAG	1 cut(s)	807
SfoI	GGCGCC	1 cut(s)	1810
SinI	GGWCC	1 cut(s)	666
SmiMI	CAYNNNNRTG	2 cut(s)	1220, 1344
SmlI	CTYRAG	1 cut(s)	1112
SmoI	CTYRAG	1 cut(s)	1112
SphI	GCATGC	1 cut(s)	63
SsiI	CCGC	3 cut(s)	986, 1038, 1983
SspDI	GGCGCC	1 cut(s)	1808
SspMI	CTAG	1 cut(s)	1548
StyD4I	CCNGG	4 cut(s)	668, 871, 1501, 1986
StyI	CCWWGG	2 cut(s)	213, 1680
TaaI	ACNGT	7 cut(s)	205, 298, 606, 636, 844, 1080, 1557
TaiI	ACGT	2 cut(s)	575, 882
TaqI	TCGA	2 cut(s)	248, 1124
TatI	WGTACW	2 cut(s)	306, 1557
TauI	GCSGC	2 cut(s)	988, 1986
TfiI	GAWTC	5 cut(s)	99, 106, 818, 1020, 1157
Tru1I	TTAA	3 cut(s)	725, 1113, 1254
Tru9I	TTAA	3 cut(s)	725, 1113, 1254
TscAI	CASTG	4 cut(s)	301, 590, 1605, 1975
TseFI	GTSAC	1 cut(s)	1080
TseI	GCWGC	3 cut(s)	56, 887, 1412
Tsp45I	GTSAC	1 cut(s)	1080
TspDTI	ATGAA	7 cut(s)	490, 691, 1012, 1204, 1301, 1528, 1603
TspGWI	ACGGA	1 cut(s)	1331
TspRI	CASTG	4 cut(s)	301, 590, 1605, 1975
Vha464I	CTTAAG	1 cut(s)	1112
VpaK11BI	GGWCC	1 cut(s)	666
XapI	RAATTY	7 cut(s)	192, 533, 540, 1610, 1689, 1748, 2002
XceI	RCATGY	3 cut(s)	63, 319, 484
XmiI	GTMKAC	1 cut(s)	638
XspI	CTAG	1 cut(s)	1548
Zsp2I	ATGCAT	2 cut(s)	1324, 1351

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.