MD09G1224900.v1.1

Acetyltransferase (GNAT) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0016888
Physical Location & Seq
Forward (+)
27683474 .. 27686383
2910 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1224900.v1.1.491

Sequence Viewer

Length: 1230 bp
ATGGTGCTAACTAAGGTAGCTGCTGAAAACTCGCCAATAAGGGTGCCTTTGCTTCAACTGGTGGTGAGAGAGTATGATGAAGAACGTGATAAGACCGCGGTGGAGGAGATGGAAAGGAAGTGTGAACTTGAGCAGTGTGGAAACCCTTCTTTAGTAACTGATCTCATGGGTGATCCTACATGTCGCATTCGCCGTTTCCCGACACACGTTATGCTGATTGCAGAGTACGGAGAACAAAATATAGTAGGTGTGATAAGGGGTTGTGTAAAAGAGGCAACATCAGGCAATTCAGTTTTTGTAAGGCTTGCTTATATTTTGGGACTTAGGGTCTCTCCCACGCACAGGAGGCTAGGCATTGGTTCAAAACTAGTCACACATTTGGAAGAATGGTGCAAGCAAAACGGAGCAAACTATGCATACATGGCCACAGACTGTACAAACGAGGCCTCTATCAATCTATTCACAATAAAATGTGCATACCAAAAGTTTCGCACACCCACAATATTGGTCCAACCAGTCCATGCACACAAGAAGCCAATATCCTCGACCAAAATTGCAATCGTTCAACTTCCAGCGAAGCTAGCCGAGTCAATCTATTGCCAACTCTTTGCCCAATCAGAATTTTTCCCCAAAGATATCCACAACATTTTATCTAGTCACCTCAATTTGGGTAGTTTCATGGCCCTACCGAAAAAGTCCCTCCTCAAGTTTGATCCACAAAAGGACACTACTCTGCCTACAAACTTTGCCATCCTCAGTGTTTGGAACACGAAAGAAGTGTTCAAGTTGCAAGTGAAGGGAGTGTCGAGCCTAACGTATGCATGTTGTGTGGGGGCTAGGGTTTTAGATGCATGGCTTCCATGGCTGCGATTGCCTTCGTTTCCCAACGTTTTTAAGCAGTTTGGGGTCCACTTCATGTACGGACTACACATGGAGGGTAAGAACGGAAAGTGGCTCATGAAGGCTTTGTGCAACTTTGTGCATAACATGGCTAGAGAAGACGATGGTTGTGCTGCTGTGGTGACTGAGGTGAGCCAGAGGGACCCAGTTAGAGAGGCAATCCCACATTGGAGAAAGTTGTCGTGGGAAGAAGATATGTGGTGCGTTAAGAAGCTTGCTGATCAAGCAAAGCAAGGAGAGAAGACGACGTCATCTGATGACGGCCAGTTTGATTGGATTAACACTCGATCTTCTTCATCGGTGGTTTTTGTTGACCCTCGTGACTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acetyltransf_1

Protein Analysis

410

Amino Acids

46.32

Weight (kDa)

8.59

Isoelectric Point (pI)

39.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acetyltransf_1 PF00583 57 - 155 2.7e-16 Acetyltransferase (GNAT) family
Acetyltransf_7 PF13508 69 - 141 3.7e-08 Acetyltransferase (GNAT) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016888)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g28600
malus_domestica MD09G1224900.v1.1
prunus_persica Prupe.3G098400_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0132941
rosa_laevigata RLG00000019294
rosa_multiflora Rmu_co8485787.1_g000001 Rmu_sc0001314.1_g000006
rosa_roxburghii Rroxscaffold_2G00111600
rosa_rugosa Rorug02G0308600
rosa_samantha Rh2AG361000 Rh2BG366900 Rh2CG344300 Rh2DG383900
rosa_wichuraiana Rw2G029360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1151
AccB1I GGYRCC 1 cut(s) 43
AccII CGCG 1 cut(s) 98
AciI CCGC 2 cut(s) 96, 98
AclI AACGTT 1 cut(s) 888
AclWI GGATC 2 cut(s) 167, 707
AcoI YGGCCR 2 cut(s) 423, 1162
AcsI RAATTY 1 cut(s) 620
AcyI GRCGYC 1 cut(s) 1148
AfaI GTAC 3 cut(s) 227, 436, 920
AfiI CCNNNNNNNGG 2 cut(s) 342, 667
AflIII ACRYGT 2 cut(s) 179, 205
AgsI TTSAA 4 cut(s) 56, 363, 566, 784
AhdI GACNNNNNGTC 1 cut(s) 326
AhlI ACTAGT 1 cut(s) 367
AluBI AGCT 3 cut(s) 20, 580, 1114
AluI AGCT 3 cut(s) 20, 580, 1114
Alw26I GTCTC 1 cut(s) 334
AlwI GGATC 2 cut(s) 167, 707
AoxI GGCC 4 cut(s) 423, 444, 681, 1162
ApeKI GCWGC 3 cut(s) 20, 865, 1013
ApoI RAATTY 1 cut(s) 620
ArsI GACNNNNNNTTYG 2 cut(s) 1151, 1183
Asp700I GAANNNNTTC 1 cut(s) 145
AspS9I GGNCC 4 cut(s) 508, 682, 907, 1042
AsuHPI GGTGA 5 cut(s) 76, 182, 650, 1033, 1042
AsuNHI GCTAGC 1 cut(s) 580
AvaII GGWCC 3 cut(s) 508, 907, 1042
BalI TGGCCA 1 cut(s) 425
BanI GGYRCC 1 cut(s) 43
BauI CACGAG 1 cut(s) 1218
BbsI GAAGAC 2 cut(s) 1005, 1148
BbvI GCAGC 3 cut(s) 7, 852, 1000
BccI CCATC 3 cut(s) 103, 758, 998
BceAI ACGGC 2 cut(s) 177, 1177
BcgI CGANNNNNNTGC 2 cut(s) 992, 1026
BclI TGATCA 1 cut(s) 1120
BcoDI GTCTC 1 cut(s) 334
BcuI ACTAGT 1 cut(s) 367
BfaI CTAG 7 cut(s) 350, 368, 581, 654, 837, 993, 1228
BisI GCNGC 3 cut(s) 21, 866, 1014
BlsI GCNGC 3 cut(s) 22, 867, 1015
Bme18I GGWCC 3 cut(s) 508, 907, 1042
BmeRI GACNNNNNGTC 1 cut(s) 326
BmgT120I GGNCC 4 cut(s) 508, 682, 907, 1042
BmiI GGNNCC 4 cut(s) 45, 908, 1043, 1044
BmrI ACTGGG 1 cut(s) 1040
BmsI GCATC 1 cut(s) 838
BmtI GCTAGC 1 cut(s) 584
BmuI ACTGGG 1 cut(s) 1040
BpiI GAAGAC 2 cut(s) 1005, 1148
BpuEI CTTGAG 2 cut(s) 149, 689
BsaHI GRCGYC 1 cut(s) 1148
BsaI GGTCTC 1 cut(s) 334
BsaJI CCNNGG 2 cut(s) 96, 860
Bsc4I CCNNNNNNNGG 2 cut(s) 342, 667
Bse1I ACTGG 4 cut(s) 63, 515, 1046, 1165
BseDI CCNNGG 2 cut(s) 96, 860
BseGI GGATG 1 cut(s) 750
BseLI CCNNNNNNNGG 2 cut(s) 342, 667
BseMII CTCAG 2 cut(s) 769, 1017
BseNI ACTGG 4 cut(s) 63, 515, 1046, 1165
BseRI GAGGAG 2 cut(s) 119, 692
BseXI GCAGC 3 cut(s) 7, 852, 1000
Bsh1236I CGCG 1 cut(s) 98
BshFI GGCC 4 cut(s) 425, 446, 683, 1164
BshNI GGYRCC 1 cut(s) 43
BslFI GGGAC 3 cut(s) 333, 682, 1055
BslI CCNNNNNNNGG 2 cut(s) 342, 667
BsmAI GTCTC 1 cut(s) 334
BsmFI GGGAC 3 cut(s) 333, 682, 1055
BsmI GAATGC 1 cut(s) 186
BsnI GGCC 4 cut(s) 425, 446, 683, 1164
Bso31I GGTCTC 1 cut(s) 334
Bsp1407I TGTACA 1 cut(s) 434
Bsp143I GATC 5 cut(s) 160, 172, 712, 1120, 1187
Bsp19I CCATGG 1 cut(s) 860
BspACI CCGC 2 cut(s) 96, 98
BspANI GGCC 4 cut(s) 425, 446, 683, 1164
BspCNI CTCAG 2 cut(s) 768, 1018
BspFNI CGCG 1 cut(s) 98
BspHI TCATGA 1 cut(s) 957
BspLI GGNNCC 4 cut(s) 45, 908, 1043, 1044
BspOI GCTAGC 1 cut(s) 584
BspPI GGATC 2 cut(s) 167, 707
BspT107I GGYRCC 1 cut(s) 43
BspTNI GGTCTC 1 cut(s) 334
BsrGI TGTACA 1 cut(s) 434
BsrI ACTGG 4 cut(s) 63, 515, 1046, 1165
BssECI CCNNGG 2 cut(s) 96, 860
BssMI GATC 5 cut(s) 160, 172, 712, 1120, 1187
BssNI GRCGYC 1 cut(s) 1148
BssSI CACGAG 1 cut(s) 1218
BssT1I CCWWGG 1 cut(s) 860
Bst2BI CACGAG 1 cut(s) 1218
Bst4CI ACNGT 1 cut(s) 434
BstACI GRCGYC 1 cut(s) 1148
BstAPI GCANNNNNTGC 1 cut(s) 413
BstAUI TGTACA 1 cut(s) 434
BstC8I GCNNGC 4 cut(s) 306, 395, 582, 1116
BstDEI CTNAG 4 cut(s) 12, 323, 755, 1026
BstDSI CCRYGG 2 cut(s) 96, 860
BstF5I GGATG 1 cut(s) 750
BstFNI CGCG 1 cut(s) 98
BstKTI GATC 5 cut(s) 163, 175, 715, 1123, 1190
BstMAI GTCTC 1 cut(s) 334
BstMBI GATC 5 cut(s) 160, 172, 712, 1120, 1187
BstMWI GCNNNNNNNGC 7 cut(s) 346, 413, 422, 581, 862, 871, 1124
BstNSI RCATGY 2 cut(s) 183, 825
BstUI CGCG 1 cut(s) 98
BstV1I GCAGC 3 cut(s) 7, 852, 1000
BstV2I GAAGAC 2 cut(s) 1005, 1148
BstXI CCANNNNNNTGG 1 cut(s) 505
BsuRI GGCC 4 cut(s) 425, 446, 683, 1164
BtgI CCRYGG 2 cut(s) 96, 860
BtsCI GGATG 1 cut(s) 750
BtsI GCAGTG 1 cut(s) 140
BtsIMutI CAGTG 2 cut(s) 140, 763
Cac8I GCNNGC 4 cut(s) 306, 395, 582, 1116
CciI TCATGA 1 cut(s) 957
Cfr13I GGNCC 4 cut(s) 508, 682, 907, 1042
Cfr42I CCGCGG 1 cut(s) 99
Csp6I GTAC 3 cut(s) 226, 435, 919
CviQI GTAC 3 cut(s) 226, 435, 919
DdeI CTNAG 4 cut(s) 12, 323, 755, 1026
DpnI GATC 5 cut(s) 162, 174, 714, 1122, 1189
DpnII GATC 5 cut(s) 160, 172, 712, 1120, 1187
DriI GACNNNNNGTC 1 cut(s) 326
EaeI YGGCCR 2 cut(s) 423, 1162
Eam1105I GACNNNNNGTC 1 cut(s) 326
Eco130I CCWWGG 1 cut(s) 860
Eco147I AGGCCT 1 cut(s) 446
Eco31I GGTCTC 1 cut(s) 334
Eco32I GATATC 1 cut(s) 637
Eco47I GGWCC 3 cut(s) 508, 907, 1042
EcoO109I RGGNCCY 1 cut(s) 1042
EcoRV GATATC 1 cut(s) 637
EcoT14I CCWWGG 1 cut(s) 860
EcoT22I ATGCAT 3 cut(s) 418, 823, 853
ErhI CCWWGG 1 cut(s) 860
FalI AAGNNNNNCTT 4 cut(s) 31, 63, 292, 324
FaqI GGGAC 3 cut(s) 333, 682, 1055
FbaI TGATCA 1 cut(s) 1120
Fnu4HI GCNGC 3 cut(s) 21, 866, 1014
FokI GGATG 1 cut(s) 737
Fsp4HI GCNGC 3 cut(s) 21, 866, 1014
FspBI CTAG 7 cut(s) 350, 368, 581, 654, 837, 993, 1228
GluI GCNGC 3 cut(s) 21, 866, 1014
HaeIII GGCC 4 cut(s) 425, 446, 683, 1164
Hin1I GRCGYC 1 cut(s) 1148
HincII GTYRAC 1 cut(s) 1213
HindII GTYRAC 1 cut(s) 1213
HindIII AAGCTT 1 cut(s) 1112
HinfI GANTC 1 cut(s) 587
HphI GGTGA 5 cut(s) 76, 182, 650, 1033, 1042
Hpy166II GTNNAC 3 cut(s) 125, 910, 1213
Hpy188I TCNGA 2 cut(s) 619, 1156
Hpy188III TCNNGA 3 cut(s) 199, 958, 1220
Hpy8I GTNNAC 3 cut(s) 125, 910, 1213
Hpy99I CGWCG 1 cut(s) 1150
HpyAV CCTTC 4 cut(s) 156, 790, 885, 955
HpyCH4III ACNGT 1 cut(s) 434
HpyCH4IV ACGT 5 cut(s) 85, 207, 815, 888, 1148
HpyF10VI GCNNNNNNNGC 7 cut(s) 346, 413, 422, 581, 862, 871, 1124
HpyF3I CTNAG 4 cut(s) 12, 323, 755, 1026
HpySE526I ACGT 5 cut(s) 85, 207, 815, 888, 1148
Hsp92I GRCGYC 1 cut(s) 1148
KflI GGGWCCC 1 cut(s) 1042
Ksp22I TGATCA 1 cut(s) 1120
KspI CCGCGG 1 cut(s) 99
Kzo9I GATC 5 cut(s) 160, 172, 712, 1120, 1187
LmnI GCTCC 1 cut(s) 404
LpnPI CCDG 8 cut(s) 44, 267, 328, 528, 585, 1049, 1059, 1178
Lsp1109I GCAGC 3 cut(s) 7, 852, 1000
LweI GCATC 1 cut(s) 838
MaeI CTAG 7 cut(s) 350, 368, 581, 654, 837, 993, 1228
MaeII ACGT 5 cut(s) 85, 207, 815, 888, 1148
MaeIII GTNAC 5 cut(s) 154, 370, 656, 1021, 1220
MalI GATC 5 cut(s) 162, 174, 714, 1122, 1189
MboI GATC 5 cut(s) 160, 172, 712, 1120, 1187
MboII GAAGA 8 cut(s) 92, 395, 1010, 1100, 1103, 1153, 1182, 1185
MlsI TGGCCA 1 cut(s) 425
MluCI AATT 4 cut(s) 286, 552, 620, 664
MluNI TGGCCA 1 cut(s) 425
MlyI GAGTC 1 cut(s) 596
MmeI TCCRAC 1 cut(s) 535
Mox20I TGGCCA 1 cut(s) 425
Mph1103I ATGCAT 3 cut(s) 418, 823, 853
MroXI GAANNNNTTC 1 cut(s) 145
MscI TGGCCA 1 cut(s) 425
MseI TTAA 3 cut(s) 894, 1107, 1179
Msp20I TGGCCA 1 cut(s) 425
MspA1I CMGCKG 1 cut(s) 98
Mva1269I GAATGC 1 cut(s) 186
MvnI CGCG 1 cut(s) 98
MwoI GCNNNNNNNGC 7 cut(s) 346, 413, 422, 581, 862, 871, 1124
NcoI CCATGG 1 cut(s) 860
NdeII GATC 5 cut(s) 160, 172, 712, 1120, 1187
NheI GCTAGC 1 cut(s) 580
NlaIV GGNNCC 4 cut(s) 45, 908, 1043, 1044
NmeAIII GCCGAG 1 cut(s) 610
NmuCI GTSAC 4 cut(s) 370, 656, 1021, 1220
NsiI ATGCAT 3 cut(s) 418, 823, 853
NspI RCATGY 2 cut(s) 183, 825
PagI TCATGA 1 cut(s) 957
PceI AGGCCT 1 cut(s) 446
PciI ACATGT 1 cut(s) 179
PcsI WCGNNNNNNNCGW 2 cut(s) 190, 812
PctI GAATGC 1 cut(s) 186
PdmI GAANNNNTTC 1 cut(s) 145
PflFI GACNNNGTC 1 cut(s) 1147
PkrI GCNGC 3 cut(s) 22, 867, 1015
PleI GAGTC 1 cut(s) 595
PpsI GAGTC 1 cut(s) 595
PpuMI RGGWCCY 1 cut(s) 1042
PscI ACATGT 1 cut(s) 179
Psp1406I AACGTT 1 cut(s) 888
Psp5II RGGWCCY 1 cut(s) 1042
PspN4I GGNNCC 4 cut(s) 45, 908, 1043, 1044
PspPI GGNCC 4 cut(s) 508, 682, 907, 1042
PspPPI RGGWCCY 1 cut(s) 1042
PsyI GACNNNGTC 1 cut(s) 1147
RsaI GTAC 3 cut(s) 227, 436, 920
RsaNI GTAC 3 cut(s) 226, 435, 919
SacII CCGCGG 1 cut(s) 99
SaqAI TTAA 3 cut(s) 894, 1107, 1179
SatI GCNGC 3 cut(s) 21, 866, 1014
Sau3AI GATC 5 cut(s) 160, 172, 712, 1120, 1187
Sau96I GGNCC 4 cut(s) 508, 682, 907, 1042
SchI GAGTC 1 cut(s) 596
SfaNI GCATC 1 cut(s) 838
Sfr303I CCGCGG 1 cut(s) 99
SgrBI CCGCGG 1 cut(s) 99
SinI GGWCC 3 cut(s) 508, 907, 1042
SmlI CTYRAG 2 cut(s) 128, 704
SmoI CTYRAG 2 cut(s) 128, 704
SpeI ACTAGT 1 cut(s) 367
Sse9I AATT 4 cut(s) 286, 552, 620, 664
SseBI AGGCCT 1 cut(s) 446
SsiI CCGC 2 cut(s) 96, 98
SspI AATATT 1 cut(s) 504
SspMI CTAG 7 cut(s) 350, 368, 581, 654, 837, 993, 1228
StuI AGGCCT 1 cut(s) 446
StyI CCWWGG 1 cut(s) 860
TaaI ACNGT 1 cut(s) 434
TaiI ACGT 5 cut(s) 88, 210, 818, 891, 1151
TaqI TCGA 3 cut(s) 545, 806, 1186
TasI AATT 4 cut(s) 286, 552, 620, 664
TatI WGTACW 1 cut(s) 434
Tru1I TTAA 3 cut(s) 894, 1107, 1179
Tru9I TTAA 3 cut(s) 894, 1107, 1179
TscAI CASTG 2 cut(s) 140, 763
TseFI GTSAC 4 cut(s) 370, 656, 1021, 1220
TseI GCWGC 3 cut(s) 20, 865, 1013
Tsp45I GTSAC 4 cut(s) 370, 656, 1021, 1220
TspDTI ATGAA 5 cut(s) 93, 667, 904, 974, 1185
TspGWI ACGGA 4 cut(s) 243, 417, 936, 960
TspRI CASTG 2 cut(s) 140, 763
Tth111I GACNNNGTC 1 cut(s) 1147
VpaK11BI GGWCC 3 cut(s) 508, 907, 1042
XapI RAATTY 1 cut(s) 620
XceI RCATGY 2 cut(s) 183, 825
XmnI GAANNNNTTC 1 cut(s) 145
XspI CTAG 7 cut(s) 350, 368, 581, 654, 837, 993, 1228
ZraI GACGTC 1 cut(s) 1149
Zsp2I ATGCAT 3 cut(s) 418, 823, 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.