MD09G1228000.v1.1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0016619
Physical Location & Seq
Forward (+)
28160141 .. 28168214
8074 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1228000.v1.1.491

Sequence Viewer

Length: 948 bp
ATGGCTGCTGGAAAACCTCAACTTTTGCAGCAGTTTGTGCTGTCTGCTTTTCTTCTCTGCCTCTGTTATTCTGCAAATGGGGTTCAGTGCTTTAAGGAGAAGAAGCTCCTCAAGCTGCAACAGTTTCGTTGGAGGCGGCATGGTCGACATGCTCAAACCACAATTACTGCTTGCCTCTCCCACAAATCAAGAAGAGAAAAAGGTGCAGCCATATTGGAGATAAAACACAAAGATGACTGCTTTGGAACTGGAAAGGTTGTGGACTGGAACAAAAAACAACGGAAACGTTTAGTTTTGGGCGATGTTCACATTCGGACACTACAATTCGGATTCAAAAACAAGGTTTCCGGCAGCATGAAAGATGTATCAGATGCTCAAATCCTGTTAACTTCCGGCGCTAGGCTACAAACACTGAACTACATTGTAACCGTGGAACTGGAAGGAAGAAATATGACACTGATTGTAGACGCAGGCAGTGATTTGACTTGGTCTCTCTTCAATCCTTTTGCATCCTCTTCGTACAAATCGGTTTTATGCAACTCCTGGACTTGTCAGTCACTCCAGTTTGATACCGGGAATCCAGGGGCATGCGGGAGCAACCCAATGAGCTGTAACTATGTGGTGAAATATGGTGATGGCTCGTACACTCATGGTGAGCTAGGTAGTGATCATCTCAGTCTTGGAACTGCGCCGGTGACCAATTTTGTGTTTGGTTGTGGCCAAAATAATAAGGGTCTGTTTGGAGGAACTTCGGGTCTAATGGGTTTGGGAAGGAGTGAAAATGTCTCAGTGGTGTCTCAAACTTCTGCATTATTTGGAGGAGTTTTTTCCTACTGCTTACCTACAACAGAATCCACAGCTTCTGGTTCTTTAATTATGGGGGGTGATGCTTCAATTTACAAGAACTCCATTCTTATTTCTTACACTAGAATGGTTAATAATCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

316

Amino Acids

34.26

Weight (kDa)

9.29

Isoelectric Point (pI)

37.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 140 - 295 2e-36 Xylanase inhibitor N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016619)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79720
fragaria_vesca FvH4_6g28220
malus_domestica MD09G1228000.v1.1
prunus_persica Prupe.3G103900_v2.0.a1
pyrus_communis pycom09g14720
rosa_chinensis RchiOBHm_Chr2g0132281
rosa_laevigata RLG00000019243
rosa_roxburghii Rroxscaffold_2G00112200
rosa_rugosa Rorug02G0303400
rosa_samantha Rh2AG356400 Rh2BG362800 Rh2CG339900 Rh2DG379700
rosa_wichuraiana Rw2G028980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 553
AccI GTMKAC 2 cut(s) 145, 465
AciI CCGC 2 cut(s) 136, 591
AclI AACGTT 1 cut(s) 286
AcoI YGGCCR 1 cut(s) 718
AfaI GTAC 2 cut(s) 521, 644
AfiI CCNNNNNNNGG 1 cut(s) 399
AgsI TTSAA 3 cut(s) 334, 499, 894
AjnI CCWGG 2 cut(s) 542, 580
AluBI AGCT 5 cut(s) 106, 115, 609, 658, 860
AluI AGCT 5 cut(s) 106, 115, 609, 658, 860
Alw26I GTCTC 3 cut(s) 495, 790, 801
AlwNI CAGNNNCTG 1 cut(s) 863
AoxI GGCC 1 cut(s) 718
ApeKI GCWGC 5 cut(s) 5, 28, 115, 206, 351
AspLEI GCGC 2 cut(s) 398, 691
AsuC2I CCSGG 1 cut(s) 574
AsuHPI GGTGA 5 cut(s) 634, 644, 665, 706, 896
BalI TGGCCA 1 cut(s) 720
BbvI GCAGC 4 cut(s) 40, 102, 218, 363
BccI CCATC 1 cut(s) 629
BcgI CGANNNNNNTGC 4 cut(s) 107, 141, 498, 532
BciT130I CCWGG 2 cut(s) 544, 582
BclI TGATCA 1 cut(s) 667
BcnI CCSGG 1 cut(s) 574
BcoDI GTCTC 3 cut(s) 495, 790, 801
BfaI CTAG 3 cut(s) 399, 659, 927
BfoI RGCGCY 1 cut(s) 399
BisI GCNGC 6 cut(s) 6, 29, 116, 137, 207, 352
BlsI GCNGC 6 cut(s) 7, 30, 117, 138, 208, 353
Bme1390I CCNGG 3 cut(s) 544, 574, 582
BmrFI CCNGG 3 cut(s) 544, 574, 582
BmsI GCATC 3 cut(s) 361, 518, 877
BpmI CTGGAG 1 cut(s) 545
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 1 cut(s) 574
BsaI GGTCTC 1 cut(s) 495
BsaJI CCNNGG 2 cut(s) 429, 581
BsaXI ACNNNNNCTCC 2 cut(s) 890, 920
Bsc4I CCNNNNNNNGG 1 cut(s) 399
Bse118I RCCGGY 1 cut(s) 691
Bse1I ACTGG 4 cut(s) 253, 269, 441, 562
BseBI CCWGG 2 cut(s) 544, 582
BseDI CCNNGG 2 cut(s) 429, 581
BseGI GGATG 1 cut(s) 509
BseLI CCNNNNNNNGG 1 cut(s) 399
BseMII CTCAG 2 cut(s) 688, 801
BseNI ACTGG 4 cut(s) 253, 269, 441, 562
BseRI GAGGAG 2 cut(s) 98, 834
BseXI GCAGC 4 cut(s) 40, 102, 218, 363
BsgI GTGCAG 1 cut(s) 225
BshFI GGCC 1 cut(s) 720
BsiSI CCGG 4 cut(s) 348, 393, 573, 692
BslI CCNNNNNNNGG 1 cut(s) 399
BsmAI GTCTC 3 cut(s) 495, 790, 801
BsnI GGCC 1 cut(s) 720
Bso31I GGTCTC 1 cut(s) 495
Bsp143I GATC 1 cut(s) 667
BspACI CCGC 2 cut(s) 136, 591
BspANI GGCC 1 cut(s) 720
BspCNI CTCAG 2 cut(s) 687, 800
BspTNI GGTCTC 1 cut(s) 495
BsrFI RCCGGY 1 cut(s) 691
BsrI ACTGG 4 cut(s) 253, 269, 441, 562
BssAI RCCGGY 1 cut(s) 691
BssECI CCNNGG 2 cut(s) 429, 581
BssMI GATC 1 cut(s) 667
Bst2UI CCWGG 2 cut(s) 544, 582
Bst4CI ACNGT 2 cut(s) 123, 430
Bst6I CTCTTC 3 cut(s) 187, 500, 520
BstAPI GCANNNNNTGC 1 cut(s) 37
BstC8I GCNNGC 3 cut(s) 172, 472, 589
BstDEI CTNAG 2 cut(s) 674, 787
BstDSI CCRYGG 1 cut(s) 429
BstEII GGTNACC 1 cut(s) 694
BstF5I GGATG 1 cut(s) 509
BstH2I RGCGCY 1 cut(s) 399
BstHHI GCGC 2 cut(s) 398, 691
BstKTI GATC 1 cut(s) 670
BstMAI GTCTC 3 cut(s) 495, 790, 801
BstMBI GATC 1 cut(s) 667
BstMWI GCNNNNNNNGC 2 cut(s) 37, 112
BstNI CCWGG 2 cut(s) 544, 582
BstNSI RCATGY 2 cut(s) 152, 591
BstPI GGTNACC 1 cut(s) 694
BstSCI CCNGG 3 cut(s) 542, 572, 580
BstV1I GCAGC 4 cut(s) 40, 102, 218, 363
BsuRI GGCC 1 cut(s) 720
BtgI CCRYGG 1 cut(s) 429
BtgZI GCGATG 1 cut(s) 315
BtsCI GGATG 1 cut(s) 509
BtsI GCAGTG 1 cut(s) 481
BtsIMutI CAGTG 5 cut(s) 92, 410, 455, 481, 795
Cac8I GCNNGC 3 cut(s) 172, 472, 589
CaiI CAGNNNCTG 1 cut(s) 863
CfoI GCGC 2 cut(s) 398, 691
Cfr10I RCCGGY 1 cut(s) 691
CseI GACGC 1 cut(s) 476
Csp6I GTAC 2 cut(s) 520, 643
CviAII CATG 5 cut(s) 140, 149, 355, 588, 650
CviQI GTAC 2 cut(s) 520, 643
DdeI CTNAG 2 cut(s) 674, 787
DpnI GATC 1 cut(s) 669
DpnII GATC 1 cut(s) 667
DrdI GACNNNNNNGTC 1 cut(s) 553
DseDI GACNNNNNNGTC 1 cut(s) 553
EaeI YGGCCR 1 cut(s) 718
Eam1104I CTCTTC 3 cut(s) 187, 500, 520
EarI CTCTTC 3 cut(s) 187, 500, 520
Eco31I GGTCTC 1 cut(s) 495
Eco91I GGTNACC 1 cut(s) 694
EcoO65I GGTNACC 1 cut(s) 694
EcoRII CCWGG 2 cut(s) 542, 580
FaeI CATG 5 cut(s) 143, 152, 358, 591, 653
FatI CATG 5 cut(s) 139, 148, 354, 587, 649
FauI CCCGC 1 cut(s) 584
FbaI TGATCA 1 cut(s) 667
FblI GTMKAC 2 cut(s) 145, 465
Fnu4HI GCNGC 6 cut(s) 6, 29, 116, 137, 207, 352
FokI GGATG 1 cut(s) 496
Fsp4HI GCNGC 6 cut(s) 6, 29, 116, 137, 207, 352
FspBI CTAG 3 cut(s) 399, 659, 927
GlaI GCGC 2 cut(s) 397, 690
GluI GCNGC 6 cut(s) 6, 29, 116, 137, 207, 352
GsuI CTGGAG 1 cut(s) 545
HaeII RGCGCY 1 cut(s) 399
HaeIII GGCC 1 cut(s) 720
HapII CCGG 4 cut(s) 348, 393, 573, 692
HgaI GACGC 1 cut(s) 476
HhaI GCGC 2 cut(s) 398, 691
Hin1II CATG 5 cut(s) 143, 152, 358, 591, 653
Hin6I GCGC 2 cut(s) 396, 689
HinP1I GCGC 2 cut(s) 396, 689
HincII GTYRAC 2 cut(s) 146, 387
HindII GTYRAC 2 cut(s) 146, 387
HinfI GANTC 3 cut(s) 330, 577, 851
HpaI GTTAAC 1 cut(s) 387
HpaII CCGG 4 cut(s) 348, 393, 573, 692
HphI GGTGA 5 cut(s) 634, 644, 665, 706, 896
Hpy166II GTNNAC 6 cut(s) 146, 262, 307, 387, 466, 645
Hpy188I TCNGA 3 cut(s) 315, 329, 370
Hpy188III TCNNGA 1 cut(s) 189
Hpy8I GTNNAC 6 cut(s) 146, 262, 307, 387, 466, 645
HpyAV CCTTC 2 cut(s) 434, 765
HpyCH4III ACNGT 2 cut(s) 123, 430
HpyCH4IV ACGT 1 cut(s) 286
HpyCH4V TGCA 7 cut(s) 28, 74, 118, 206, 509, 537, 809
HpyF10VI GCNNNNNNNGC 2 cut(s) 37, 112
HpyF3I CTNAG 2 cut(s) 674, 787
HpySE526I ACGT 1 cut(s) 286
Hsp92II CATG 5 cut(s) 143, 152, 358, 591, 653
HspAI GCGC 2 cut(s) 396, 689
Ksp22I TGATCA 1 cut(s) 667
KspAI GTTAAC 1 cut(s) 387
Kzo9I GATC 1 cut(s) 667
LmnI GCTCC 2 cut(s) 111, 594
Lsp1109I GCAGC 4 cut(s) 40, 102, 218, 363
LweI GCATC 3 cut(s) 361, 518, 877
MaeI CTAG 3 cut(s) 399, 659, 927
MaeII ACGT 1 cut(s) 286
MaeIII GTNAC 4 cut(s) 424, 555, 611, 694
MalI GATC 1 cut(s) 669
MboI GATC 1 cut(s) 667
MboII GAAGA 6 cut(s) 44, 112, 204, 456, 487, 507
MlsI TGGCCA 1 cut(s) 720
MluCI AATT 5 cut(s) 162, 323, 700, 873, 894
MluNI TGGCCA 1 cut(s) 720
MmeI TCCRAC 1 cut(s) 110
MnlI CCTC 8 cut(s) 27, 71, 119, 126, 185, 523, 737, 812
Mox20I TGGCCA 1 cut(s) 720
MscI TGGCCA 1 cut(s) 720
MseI TTAA 4 cut(s) 93, 386, 872, 936
MslI CAYNNNNRTG 2 cut(s) 231, 929
Msp20I TGGCCA 1 cut(s) 720
MspI CCGG 4 cut(s) 348, 393, 573, 692
MspR9I CCNGG 3 cut(s) 544, 574, 582
MvaI CCWGG 2 cut(s) 544, 582
MwoI GCNNNNNNNGC 2 cut(s) 37, 112
NciI CCSGG 1 cut(s) 574
NdeII GATC 1 cut(s) 667
NlaIII CATG 5 cut(s) 143, 152, 358, 591, 653
NmuCI GTSAC 2 cut(s) 555, 694
NspI RCATGY 2 cut(s) 152, 591
PaeI GCATGC 1 cut(s) 591
PfeI GAWTC 3 cut(s) 330, 577, 851
PflFI GACNNNGTC 1 cut(s) 487
PfoI TCCNGGA 1 cut(s) 542
PkrI GCNGC 6 cut(s) 7, 30, 117, 138, 208, 353
Psp1406I AACGTT 1 cut(s) 286
Psp6I CCWGG 2 cut(s) 542, 580
PspEI GGTNACC 1 cut(s) 694
PspGI CCWGG 2 cut(s) 542, 580
PstNI CAGNNNCTG 1 cut(s) 863
PsyI GACNNNGTC 1 cut(s) 487
RsaI GTAC 2 cut(s) 521, 644
RsaNI GTAC 2 cut(s) 520, 643
RseI CAYNNNNRTG 2 cut(s) 231, 929
SalI GTCGAC 1 cut(s) 144
SaqAI TTAA 4 cut(s) 93, 386, 872, 936
SatI GCNGC 6 cut(s) 6, 29, 116, 137, 207, 352
Sau3AI GATC 1 cut(s) 667
ScrFI CCNGG 3 cut(s) 544, 574, 582
SfaNI GCATC 3 cut(s) 361, 518, 877
SgrAI CRCCGGYG 1 cut(s) 691
SmiMI CAYNNNNRTG 2 cut(s) 231, 929
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
SphI GCATGC 1 cut(s) 591
Sse9I AATT 5 cut(s) 162, 323, 700, 873, 894
SsiI CCGC 2 cut(s) 136, 591
SspMI CTAG 3 cut(s) 399, 659, 927
StyD4I CCNGG 3 cut(s) 542, 572, 580
TaaI ACNGT 2 cut(s) 123, 430
TaiI ACGT 1 cut(s) 289
TaqI TCGA 1 cut(s) 145
TasI AATT 5 cut(s) 162, 323, 700, 873, 894
TauI GCSGC 1 cut(s) 139
TfiI GAWTC 3 cut(s) 330, 577, 851
Tru1I TTAA 4 cut(s) 93, 386, 872, 936
Tru9I TTAA 4 cut(s) 93, 386, 872, 936
TscAI CASTG 5 cut(s) 92, 417, 462, 481, 795
TseFI GTSAC 2 cut(s) 555, 694
TseI GCWGC 5 cut(s) 5, 28, 115, 206, 351
Tsp45I GTSAC 2 cut(s) 555, 694
TspDTI ATGAA 1 cut(s) 371
TspGWI ACGGA 1 cut(s) 295
TspRI CASTG 5 cut(s) 92, 417, 462, 481, 795
Tth111I GACNNNGTC 1 cut(s) 487
XceI RCATGY 2 cut(s) 152, 591
XmiI GTMKAC 2 cut(s) 145, 465
XspI CTAG 3 cut(s) 399, 659, 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.