pycom09g14720

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0016619
Physical Location & Seq
Forward (+)
14457856 .. 14459773
1918 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g14720.1

Sequence Viewer

Length: 1467 bp
ATGGCTGCTGCAAAACCTCAACTTTTGCAGCAGTTTGTGCTGTCTGCTTTTCTTCTCTGCCTCTGTTATTCTGCAAATGGGGTTCAGTGCTTTAAAGAGAAGAAGCTCCTCAAGCTGCAACAGTTTCGTTGGAGGCAGCATGGTCGACGTGCTGGAACCACAACTACTGCTTGCCTCTCCCACAAATCAAGAAGAGAAAAAGGTGCGGCCATATTGGAGATAAAACACAGAGATGGCTGCTCTGGAACTGGAAAGATTGTGGACTGGAACAAAAAACAACAGAAACGTTTAGTTTTGGACGATGTTCACGTTCGGACACTACAATCAGGATTCAAAAACAGGGTTTCCGGCAGCATGGAAGATGTATCAGATGCTCAAATCCTGTTAACTTCCGGCGCTAGGCTACAAACACTGAACTACATTGTAACCGTGGAACTGGGAGGAAGAAATATGACACTGATTGTAGACACAGGCAGTGATTTGACTTGGGTTCAATGCCAGCCTTGCAAATTGTGTTATAATCAACAAGAGCCTCTCTTCAATCCTTCTGCATCCTCTTCGTACAAATCGGTTTTATGCAACTCATCGACTTGTCAGTCACTCCAGTTTGATACCGGGAATCCAGGGGCATGCGGGAGCAACCCAATGAGCTGTAACTATATCGTGAAATATGGTGATGGCTCGTACACTCGCGGTGAGCTAGGTAGTGATCGTCTCAGTCTTGGAACTGCGCCGGTGACCAATTTTGTGTTTGGTTGTGGCAGAAATAATAAGGGTCTGTTTGGAGGAACTTCGGGTCTAATGGGTTTGGGAAGGAGTGAAAGTGTCTCAGTGGTGTCTCAAACTTCTGCATTATTCGGAGGAGTTTTTTCCTACTGCTTACCTACAACTGAATCCACAGCTTCTGGTTCTTTAATTATGGGGGGTGATGCTTCAATTTACAAGAACTCCACTCCTATTTCTTACACTAGAATGGTTAATAATCCACAGCTATCATCCTTCTATATTCTCAACCTTACTGGTATAAGTATCGGGGGAGTCGCTTTACAAGCTTCAAGTTTTGCAAGTGGTGGGATTCTGATTGATTCTGGGACAGTTATTTCAAGACTGCCTCCTTCAGTTTACAAGGCTGTCAAGACAGAGTTTTTGAAACAGTTTTCTGGGTACCCTACGGCACCAGGTTTTTCGATCTTGGACACTTGCTTCAACCTGAGTGCATATCAGGAAGTGAACATTCCTACCCTCAAGTTCCACTATGAGGGAGATACTGAGATGAATGTTGATGTAACTGGAATTTTCTATCTCGTACAGACAGATGGATCCCAGATCTGCTTGGCTCTAGCAAGTCTTTCGTACGAAGATGAGATTGCTATTATCGGGAATTATCAGCAAAAAAACCAAAGGGTTATATACAATACCAAAGACTCCAAGCTGGGATTTGCAAAAGAATCATGCAGTTTTACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

489

Amino Acids

52.9

Weight (kDa)

8.78

Isoelectric Point (pI)

38.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 140 - 309 3.5e-50 Xylanase inhibitor N-terminal
Asp PF00026 140 - 190 2.4e-06 Eukaryotic aspartyl protease
Asp PF00026 219 - 482 1.4e-16 Eukaryotic aspartyl protease
TAXi_C PF14541 335 - 482 5.7e-27 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016619)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79720
fragaria_vesca FvH4_6g28220
malus_domestica MD09G1228000.v1.1
prunus_persica Prupe.3G103900_v2.0.a1
pyrus_communis pycom09g14720
rosa_chinensis RchiOBHm_Chr2g0132281
rosa_laevigata RLG00000019243
rosa_roxburghii Rroxscaffold_2G00112200
rosa_rugosa Rorug02G0303400
rosa_samantha Rh2AG356400 Rh2BG362800 Rh2CG339900 Rh2DG379700
rosa_wichuraiana Rw2G028980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 519
AasI GACNNNNNNGTC 1 cut(s) 595
Acc65I GGTACC 1 cut(s) 1164
AccB1I GGYRCC 2 cut(s) 1164, 1174
AccI GTMKAC 2 cut(s) 145, 465
AccII CGCG 1 cut(s) 693
AciI CCGC 3 cut(s) 206, 633, 693
AclI AACGTT 1 cut(s) 286
AclWI GGATC 2 cut(s) 1314, 1327
AcoI YGGCCR 1 cut(s) 207
AcsI RAATTY 1 cut(s) 1293
AcuI CTGAAG 1 cut(s) 1101
AfaI GTAC 5 cut(s) 563, 686, 1166, 1308, 1355
AfiI CCNNNNNNNGG 2 cut(s) 399, 1258
AgsI TTSAA 8 cut(s) 334, 494, 541, 936, 1056, 1104, 1150, 1207
AjiI CACGTC 1 cut(s) 149
AjnI CCWGG 2 cut(s) 622, 1177
AluBI AGCT 8 cut(s) 106, 115, 651, 700, 902, 991, 1052, 1432
AluI AGCT 8 cut(s) 106, 115, 651, 700, 902, 991, 1052, 1432
Alw26I GTCTC 3 cut(s) 719, 832, 843
AlwI GGATC 2 cut(s) 1314, 1327
AlwNI CAGNNNCTG 1 cut(s) 905
AoxI GGCC 1 cut(s) 207
ApeKI GCWGC 7 cut(s) 5, 8, 28, 115, 136, 237, 351
ApoI RAATTY 1 cut(s) 1293
ArsI GACNNNNNNTTYG 2 cut(s) 1129, 1161
Asp718I GGTACC 1 cut(s) 1164
AspLEI GCGC 2 cut(s) 398, 733
AsuC2I CCSGG 1 cut(s) 616
AsuHPI GGTGA 4 cut(s) 686, 707, 748, 938
BamHI GGATCC 1 cut(s) 1319
BanI GGYRCC 2 cut(s) 1164, 1174
BbvI GCAGC 5 cut(s) 40, 102, 148, 224, 363
BccI CCATC 3 cut(s) 227, 671, 1310
BceAI ACGGC 1 cut(s) 1188
BcgI CGANNNNNNTGC 8 cut(s) 107, 125, 141, 159, 540, 574, 1332, 1366
BciT130I CCWGG 2 cut(s) 624, 1179
BcnI CCSGG 1 cut(s) 616
BcoDI GTCTC 3 cut(s) 719, 832, 843
BfaI CTAG 4 cut(s) 399, 701, 969, 1340
BfoI RGCGCY 1 cut(s) 399
BglII AGATCT 1 cut(s) 1326
BisI GCNGC 8 cut(s) 6, 9, 29, 116, 137, 207, 238, 352
BlsI GCNGC 8 cut(s) 7, 10, 30, 117, 138, 208, 239, 353
Bme1390I CCNGG 3 cut(s) 616, 624, 1179
BmgBI CACGTC 1 cut(s) 149
BmiI GGNNCC 4 cut(s) 157, 1166, 1176, 1321
BmrFI CCNGG 3 cut(s) 616, 624, 1179
BmrI ACTGGG 1 cut(s) 446
BmsI GCATC 3 cut(s) 361, 560, 919
BmuI ACTGGG 1 cut(s) 446
BpmI CTGGAG 1 cut(s) 587
BpuEI CTTGAG 2 cut(s) 95, 1229
BpuMI CCSGG 1 cut(s) 616
BsaAI YACGTR 1 cut(s) 1464
BsaJI CCNNGG 2 cut(s) 429, 623
BsaXI ACNNNNNCTCC 4 cut(s) 808, 838, 932, 962
Bsc4I CCNNNNNNNGG 2 cut(s) 399, 1258
Bse118I RCCGGY 1 cut(s) 733
Bse1I ACTGG 6 cut(s) 253, 269, 441, 604, 1024, 1294
BseBI CCWGG 2 cut(s) 624, 1179
BseDI CCNNGG 2 cut(s) 429, 623
BseGI GGATG 2 cut(s) 551, 995
BseLI CCNNNNNNNGG 2 cut(s) 399, 1258
BseMII CTCAG 4 cut(s) 730, 843, 1202, 1260
BseNI ACTGG 6 cut(s) 253, 269, 441, 604, 1024, 1294
BseRI GAGGAG 2 cut(s) 98, 876
BseXI GCAGC 5 cut(s) 40, 102, 148, 224, 363
BseYI CCCAGC 1 cut(s) 1432
Bsh1236I CGCG 1 cut(s) 693
BshFI GGCC 1 cut(s) 209
BshNI GGYRCC 2 cut(s) 1164, 1174
BsiSI CCGG 4 cut(s) 348, 393, 615, 734
BsiWI CGTACG 1 cut(s) 1353
BslFI GGGAC 1 cut(s) 1105
BslI CCNNNNNNNGG 2 cut(s) 399, 1258
BsmAI GTCTC 3 cut(s) 719, 832, 843
BsmBI CGTCTC 1 cut(s) 719
BsmFI GGGAC 1 cut(s) 1105
BsnI GGCC 1 cut(s) 209
Bsp143I GATC 4 cut(s) 709, 1188, 1319, 1326
BspACI CCGC 3 cut(s) 206, 633, 693
BspANI GGCC 1 cut(s) 209
BspCNI CTCAG 4 cut(s) 729, 842, 1203, 1261
BspFNI CGCG 1 cut(s) 693
BspLI GGNNCC 4 cut(s) 157, 1166, 1176, 1321
BspPI GGATC 2 cut(s) 1314, 1327
BspT107I GGYRCC 2 cut(s) 1164, 1174
BsrFI RCCGGY 1 cut(s) 733
BsrI ACTGG 6 cut(s) 253, 269, 441, 604, 1024, 1294
BssAI RCCGGY 1 cut(s) 733
BssECI CCNNGG 2 cut(s) 429, 623
BssMI GATC 4 cut(s) 709, 1188, 1319, 1326
Bst2UI CCWGG 2 cut(s) 624, 1179
Bst4CI ACNGT 4 cut(s) 123, 430, 1096, 1155
Bst6I CTCTTC 3 cut(s) 187, 542, 562
BstAPI GCANNNNNTGC 1 cut(s) 37
BstBAI YACGTR 1 cut(s) 1464
BstC8I GCNNGC 3 cut(s) 172, 500, 631
BstDEI CTNAG 4 cut(s) 716, 829, 1211, 1269
BstDSI CCRYGG 1 cut(s) 429
BstEII GGTNACC 1 cut(s) 736
BstF5I GGATG 2 cut(s) 551, 995
BstFNI CGCG 1 cut(s) 693
BstH2I RGCGCY 1 cut(s) 399
BstHHI GCGC 2 cut(s) 398, 733
BstKTI GATC 4 cut(s) 712, 1191, 1322, 1329
BstMAI GTCTC 3 cut(s) 719, 832, 843
BstMBI GATC 4 cut(s) 709, 1188, 1319, 1326
BstMWI GCNNNNNNNGC 4 cut(s) 37, 112, 504, 1049
BstNI CCWGG 2 cut(s) 624, 1179
BstNSI RCATGY 1 cut(s) 633
BstPI GGTNACC 1 cut(s) 736
BstSCI CCNGG 3 cut(s) 614, 622, 1177
BstSNI TACGTA 1 cut(s) 1464
BstUI CGCG 1 cut(s) 693
BstV1I GCAGC 5 cut(s) 40, 102, 148, 224, 363
BstX2I RGATCY 2 cut(s) 1319, 1326
BstYI RGATCY 2 cut(s) 1319, 1326
BsuRI GGCC 1 cut(s) 209
BtgI CCRYGG 1 cut(s) 429
BtrI CACGTC 1 cut(s) 149
BtsCI GGATG 2 cut(s) 551, 995
BtsI GCAGTG 1 cut(s) 481
BtsIMutI CAGTG 5 cut(s) 92, 410, 455, 481, 837
Cac8I GCNNGC 3 cut(s) 172, 500, 631
CaiI CAGNNNCTG 1 cut(s) 905
CfoI GCGC 2 cut(s) 398, 733
Cfr10I RCCGGY 1 cut(s) 733
CsiI ACCWGGT 1 cut(s) 1177
Csp6I GTAC 5 cut(s) 562, 685, 1165, 1307, 1354
CviAII CATG 4 cut(s) 140, 355, 630, 1452
CviQI GTAC 5 cut(s) 562, 685, 1165, 1307, 1354
DdeI CTNAG 4 cut(s) 716, 829, 1211, 1269
DpnI GATC 4 cut(s) 711, 1190, 1321, 1328
DpnII GATC 4 cut(s) 709, 1188, 1319, 1326
DraI TTTAAA 1 cut(s) 94
DrdI GACNNNNNNGTC 1 cut(s) 595
DseDI GACNNNNNNGTC 1 cut(s) 595
EaeI YGGCCR 1 cut(s) 207
Eam1104I CTCTTC 3 cut(s) 187, 542, 562
EarI CTCTTC 3 cut(s) 187, 542, 562
Eco105I TACGTA 1 cut(s) 1464
Eco57I CTGAAG 1 cut(s) 1101
Eco91I GGTNACC 1 cut(s) 736
EcoO65I GGTNACC 1 cut(s) 736
EcoRII CCWGG 2 cut(s) 622, 1177
Esp3I CGTCTC 1 cut(s) 719
FaeI CATG 4 cut(s) 143, 358, 633, 1455
FaqI GGGAC 1 cut(s) 1105
FatI CATG 4 cut(s) 139, 354, 629, 1451
FauI CCCGC 1 cut(s) 626
FblI GTMKAC 2 cut(s) 145, 465
Fnu4HI GCNGC 8 cut(s) 6, 9, 29, 116, 137, 207, 238, 352
FokI GGATG 2 cut(s) 538, 982
Fsp4HI GCNGC 8 cut(s) 6, 9, 29, 116, 137, 207, 238, 352
FspBI CTAG 4 cut(s) 399, 701, 969, 1340
GlaI GCGC 2 cut(s) 397, 732
GluI GCNGC 8 cut(s) 6, 9, 29, 116, 137, 207, 238, 352
GsaI CCCAGC 1 cut(s) 1436
GsuI CTGGAG 1 cut(s) 587
HaeII RGCGCY 1 cut(s) 399
HaeIII GGCC 1 cut(s) 209
HapII CCGG 4 cut(s) 348, 393, 615, 734
HhaI GCGC 2 cut(s) 398, 733
Hin1II CATG 4 cut(s) 143, 358, 633, 1455
Hin6I GCGC 2 cut(s) 396, 731
HinP1I GCGC 2 cut(s) 396, 731
HincII GTYRAC 2 cut(s) 146, 387
HindII GTYRAC 2 cut(s) 146, 387
HindIII AAGCTT 1 cut(s) 1050
HinfI GANTC 8 cut(s) 330, 619, 893, 1038, 1075, 1085, 1424, 1448
HpaI GTTAAC 1 cut(s) 387
HpaII CCGG 4 cut(s) 348, 393, 615, 734
HphI GGTGA 4 cut(s) 686, 707, 748, 938
Hpy166II GTNNAC 8 cut(s) 146, 262, 307, 387, 466, 687, 1123, 1231
Hpy188I TCNGA 4 cut(s) 315, 370, 860, 1080
Hpy188III TCNNGA 8 cut(s) 189, 243, 327, 664, 1104, 1135, 1223, 1378
Hpy8I GTNNAC 8 cut(s) 146, 262, 307, 387, 466, 687, 1123, 1231
Hpy99I CGWCG 1 cut(s) 150
HpyAV CCTTC 4 cut(s) 555, 807, 1009, 1125
HpyCH4III ACNGT 4 cut(s) 123, 430, 1096, 1155
HpyCH4IV ACGT 4 cut(s) 148, 286, 309, 1463
HpyF10VI GCNNNNNNNGC 4 cut(s) 37, 112, 504, 1049
HpyF3I CTNAG 4 cut(s) 716, 829, 1211, 1269
HpySE526I ACGT 4 cut(s) 148, 286, 309, 1463
Hsp92II CATG 4 cut(s) 143, 358, 633, 1455
HspAI GCGC 2 cut(s) 396, 731
KpnI GGTACC 1 cut(s) 1168
KspAI GTTAAC 1 cut(s) 387
Kzo9I GATC 4 cut(s) 709, 1188, 1319, 1326
LmnI GCTCC 2 cut(s) 111, 636
Lsp1109I GCAGC 5 cut(s) 40, 102, 148, 224, 363
LweI GCATC 3 cut(s) 361, 560, 919
MabI ACCWGGT 1 cut(s) 1177
MaeI CTAG 4 cut(s) 399, 701, 969, 1340
MaeII ACGT 4 cut(s) 148, 286, 309, 1463
MaeIII GTNAC 5 cut(s) 424, 597, 653, 736, 1285
MalI GATC 4 cut(s) 711, 1190, 1321, 1328
MboI GATC 4 cut(s) 709, 1188, 1319, 1326
MboII GAAGA 8 cut(s) 44, 112, 204, 371, 456, 529, 549, 1370
MflI RGATCY 2 cut(s) 1319, 1326
MluCI AATT 6 cut(s) 509, 742, 915, 936, 1293, 1381
MlyI GAGTC 2 cut(s) 1047, 1418
MmeI TCCRAC 1 cut(s) 110
MseI TTAA 4 cut(s) 93, 386, 914, 978
MslI CAYNNNNRTG 2 cut(s) 231, 971
MspI CCGG 4 cut(s) 348, 393, 615, 734
MspR9I CCNGG 3 cut(s) 616, 624, 1179
MvaI CCWGG 2 cut(s) 624, 1179
MvnI CGCG 1 cut(s) 693
MwoI GCNNNNNNNGC 4 cut(s) 37, 112, 504, 1049
NciI CCSGG 1 cut(s) 616
NdeII GATC 4 cut(s) 709, 1188, 1319, 1326
NlaIII CATG 4 cut(s) 143, 358, 633, 1455
NlaIV GGNNCC 4 cut(s) 157, 1166, 1176, 1321
NmuCI GTSAC 2 cut(s) 597, 736
NspI RCATGY 1 cut(s) 633
PaeI GCATGC 1 cut(s) 633
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PfeI GAWTC 6 cut(s) 330, 619, 893, 1075, 1085, 1448
Pfl23II CGTACG 1 cut(s) 1353
PkrI GCNGC 8 cut(s) 7, 10, 30, 117, 138, 208, 239, 353
PleI GAGTC 2 cut(s) 1046, 1418
PpsI GAGTC 2 cut(s) 1046, 1418
Ppu21I YACGTR 1 cut(s) 1464
PsiI TTATAA 1 cut(s) 519
Psp1406I AACGTT 1 cut(s) 286
Psp6I CCWGG 2 cut(s) 622, 1177
PspEI GGTNACC 1 cut(s) 736
PspFI CCCAGC 1 cut(s) 1432
PspGI CCWGG 2 cut(s) 622, 1177
PspLI CGTACG 1 cut(s) 1353
PspN4I GGNNCC 4 cut(s) 157, 1166, 1176, 1321
PsrI GAACNNNNNNTAC 2 cut(s) 148, 180
PstNI CAGNNNCTG 1 cut(s) 905
PsuI RGATCY 2 cut(s) 1319, 1326
RsaI GTAC 5 cut(s) 563, 686, 1166, 1308, 1355
RsaNI GTAC 5 cut(s) 562, 685, 1165, 1307, 1354
RseI CAYNNNNRTG 2 cut(s) 231, 971
SalI GTCGAC 1 cut(s) 144
SaqAI TTAA 4 cut(s) 93, 386, 914, 978
SatI GCNGC 8 cut(s) 6, 9, 29, 116, 137, 207, 238, 352
Sau3AI GATC 4 cut(s) 709, 1188, 1319, 1326
SchI GAGTC 2 cut(s) 1047, 1418
ScrFI CCNGG 3 cut(s) 616, 624, 1179
SexAI ACCWGGT 1 cut(s) 1177
SfaNI GCATC 3 cut(s) 361, 560, 919
SgrAI CRCCGGYG 1 cut(s) 733
SmiMI CAYNNNNRTG 2 cut(s) 231, 971
SmlI CTYRAG 2 cut(s) 110, 1244
SmoI CTYRAG 2 cut(s) 110, 1244
SnaBI TACGTA 1 cut(s) 1464
SphI GCATGC 1 cut(s) 633
Sse9I AATT 6 cut(s) 509, 742, 915, 936, 1293, 1381
SsiI CCGC 3 cut(s) 206, 633, 693
SspMI CTAG 4 cut(s) 399, 701, 969, 1340
StyD4I CCNGG 3 cut(s) 614, 622, 1177
TaaI ACNGT 4 cut(s) 123, 430, 1096, 1155
TaiI ACGT 4 cut(s) 151, 289, 312, 1466
TaqI TCGA 3 cut(s) 145, 587, 1187
TasI AATT 6 cut(s) 509, 742, 915, 936, 1293, 1381
TauI GCSGC 1 cut(s) 209
TfiI GAWTC 6 cut(s) 330, 619, 893, 1075, 1085, 1448
Tru1I TTAA 4 cut(s) 93, 386, 914, 978
Tru9I TTAA 4 cut(s) 93, 386, 914, 978
TscAI CASTG 5 cut(s) 92, 417, 462, 481, 837
TseFI GTSAC 2 cut(s) 597, 736
TseI GCWGC 7 cut(s) 5, 8, 28, 115, 136, 237, 351
Tsp45I GTSAC 2 cut(s) 597, 736
TspDTI ATGAA 1 cut(s) 1289
TspRI CASTG 5 cut(s) 92, 417, 462, 481, 837
XapI RAATTY 1 cut(s) 1293
XceI RCATGY 1 cut(s) 633
XmiI GTMKAC 2 cut(s) 145, 465
XspI CTAG 4 cut(s) 399, 701, 969, 1340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.