Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0013184

Physical Location & Seq

Genomic Coordinates Reverse (-)

35484212 .. 35486398

Gene Structure 2187 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1278200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 360 bp

ATGAGGAACGCCCAATCGGAACCAGCCCAGATTCCGACGAGCTTCGGCCACGAGCTTAGGGCTTGCCTCCGTTGCCGCCTCGTCAAGACCTACGATCAGTTCAGAGAACATGGATGCGAGAACTGCCCTTTCTTCGAGATGAACGACGACAACGAGCGCATCACCGATTGCACCACTCCTAATTTCAACGGGATCATCTCAGTAATGGATCCGAGTAGGAGTTGGGCTGCGCGGTGGCTCCGAATTGCAAAATTTGTCCCTGGTTGTTACACACTCGCTGTTTCAGAGGCTCTTTCGGAGGATATGAAGAACATATGCGAAGATGCTCAGATGCAATACGTACCGCCAAAGCGTATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000993 GO:0001098 GO:0001099 GO:0003674 GO:0003676 GO:0003700 GO:0003723 GO:0003727 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006325 GO:0006355 GO:0006357 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0008023 GO:0008150 GO:0008152 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016043 GO:0016070 GO:0016071 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032044 GO:0032784 GO:0032991 GO:0034243 GO:0034641 GO:0043170 GO:0043175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070063 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140110 GO:1901360 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Spt4

Protein Analysis

120

Amino Acids

13.72

Weight (kDa)

5.66

Isoelectric Point (pI)

58.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spt4	PF06093	19 - 95	1.4e-32	Spt4/RpoE2 zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0013184)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08565 AT5G08565 AT5G63670
fragaria_vesca	FvH4_4g10630
malus_domestica	MD04G1047800.v1.1 MD07G1114100.v1.1 MD09G1278200.v1.1 MD17G1271200.v1.1
prunus_persica	Prupe.3G015100_v2.0.a1
pyrus_communis	pycom04g04180
rosa_chinensis	RchiOBHm_Chr4g0406241
rosa_laevigata	RLG00000008786
rosa_multiflora	Rmu_ssc0000456.1_g000008
rosa_roxburghii	Rroxscaffold_5G00350980
rosa_rugosa	Rorug04G0067100
rosa_samantha	Rh4BG134100
rosa_wichuraiana	Rw4G011070 Rw4G011210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	232
AciI	CCGC	3 cut(s)	76, 232, 344
AclWI	GGATC	3 cut(s)	200, 203, 216
AcoI	YGGCCR	1 cut(s)	46
AcsI	RAATTY	1 cut(s)	251
AfaI	GTAC	1 cut(s)	342
AgsI	TTSAA	1 cut(s)	187
AjnI	CCWGG	1 cut(s)	259
AluBI	AGCT	2 cut(s)	42, 55
AluI	AGCT	2 cut(s)	42, 55
AlwI	GGATC	3 cut(s)	200, 203, 216
AoxI	GGCC	1 cut(s)	46
ApeKI	GCWGC	1 cut(s)	227
ApoI	RAATTY	1 cut(s)	251
AspLEI	GCGC	2 cut(s)	159, 232
AsuHPI	GGTGA	1 cut(s)	154
BamHI	GGATCC	1 cut(s)	208
BauI	CACGAG	1 cut(s)	50
BbvI	GCAGC	1 cut(s)	214
BciT130I	CCWGG	1 cut(s)	261
BisI	GCNGC	2 cut(s)	76, 228
BlsI	GCNGC	2 cut(s)	77, 229
Bme1390I	CCNGG	1 cut(s)	261
BmiI	GGNNCC	3 cut(s)	21, 210, 239
BmrFI	CCNGG	1 cut(s)	261
BmsI	GCATC	4 cut(s)	104, 168, 313, 321
Bpu10I	CCTNAGC	1 cut(s)	56
BsaAI	YACGTR	1 cut(s)	340
BsaJI	CCNNGG	1 cut(s)	259
BseBI	CCWGG	1 cut(s)	261
BseDI	CCNNGG	1 cut(s)	259
BseGI	GGATG	1 cut(s)	119
BseMII	CTCAG	2 cut(s)	213, 341
BseXI	GCAGC	1 cut(s)	214
Bsh1236I	CGCG	1 cut(s)	232
BshFI	GGCC	1 cut(s)	48
BslFI	GGGAC	1 cut(s)	242
BsmFI	GGGAC	1 cut(s)	242
BsnI	GGCC	1 cut(s)	48
Bsp143I	GATC	3 cut(s)	94, 192, 208
BspACI	CCGC	3 cut(s)	76, 232, 344
BspANI	GGCC	1 cut(s)	48
BspCNI	CTCAG	2 cut(s)	212, 340
BspFNI	CGCG	1 cut(s)	232
BspLI	GGNNCC	3 cut(s)	21, 210, 239
BspPI	GGATC	3 cut(s)	200, 203, 216
BssECI	CCNNGG	1 cut(s)	259
BssMI	GATC	3 cut(s)	94, 192, 208
BssSI	CACGAG	1 cut(s)	50
Bst2BI	CACGAG	1 cut(s)	50
Bst2UI	CCWGG	1 cut(s)	261
BstBAI	YACGTR	1 cut(s)	340
BstC8I	GCNNGC	1 cut(s)	64
BstDEI	CTNAG	3 cut(s)	56, 199, 327
BstF5I	GGATG	1 cut(s)	119
BstFNI	CGCG	1 cut(s)	232
BstHHI	GCGC	2 cut(s)	159, 232
BstKTI	GATC	3 cut(s)	97, 195, 211
BstMBI	GATC	3 cut(s)	94, 192, 208
BstMWI	GCNNNNNNNGC	2 cut(s)	72, 123
BstNI	CCWGG	1 cut(s)	261
BstSCI	CCNGG	1 cut(s)	259
BstSNI	TACGTA	1 cut(s)	340
BstUI	CGCG	1 cut(s)	232
BstV1I	GCAGC	1 cut(s)	214
BstX2I	RGATCY	1 cut(s)	208
BstYI	RGATCY	1 cut(s)	208
BsuRI	GGCC	1 cut(s)	48
BtsCI	GGATG	1 cut(s)	119
Cac8I	GCNNGC	1 cut(s)	64
CfoI	GCGC	2 cut(s)	159, 232
Csp6I	GTAC	1 cut(s)	341
CviAII	CATG	1 cut(s)	110
CviJI	RGCY	8 cut(s)	26, 42, 48, 55, 62, 227, 238, 290
CviKI_1	RGCY	8 cut(s)	26, 42, 48, 55, 62, 227, 238, 290
CviQI	GTAC	1 cut(s)	341
DdeI	CTNAG	3 cut(s)	56, 199, 327
DpnI	GATC	3 cut(s)	96, 194, 210
DpnII	GATC	3 cut(s)	94, 192, 208
EaeI	YGGCCR	1 cut(s)	46
Eco105I	TACGTA	1 cut(s)	340
EcoRII	CCWGG	1 cut(s)	259
FaeI	CATG	1 cut(s)	113
FaiI	YATR	6 cut(s)	111, 305, 314, 316, 356, 358
FaqI	GGGAC	1 cut(s)	242
FatI	CATG	1 cut(s)	109
FauNDI	CATATG	1 cut(s)	314
Fnu4HI	GCNGC	2 cut(s)	76, 228
FokI	GGATG	1 cut(s)	126
Fsp4HI	GCNGC	2 cut(s)	76, 228
GlaI	GCGC	2 cut(s)	158, 231
GluI	GCNGC	2 cut(s)	76, 228
HaeIII	GGCC	1 cut(s)	48
HhaI	GCGC	2 cut(s)	159, 232
Hin1II	CATG	1 cut(s)	113
Hin6I	GCGC	2 cut(s)	157, 230
HinP1I	GCGC	2 cut(s)	157, 230
HinfI	GANTC	1 cut(s)	31
HphI	GGTGA	1 cut(s)	154
Hpy188I	TCNGA	8 cut(s)	19, 36, 104, 213, 242, 286, 298, 330
Hpy188III	TCNNGA	2 cut(s)	85, 136
Hpy99I	CGWCG	2 cut(s)	40, 149
HpyCH4IV	ACGT	1 cut(s)	339
HpyCH4V	TGCA	3 cut(s)	171, 248, 334
HpyF10VI	GCNNNNNNNGC	2 cut(s)	72, 123
HpyF3I	CTNAG	3 cut(s)	56, 199, 327
HpySE526I	ACGT	1 cut(s)	339
Hsp92II	CATG	1 cut(s)	113
HspAI	GCGC	2 cut(s)	157, 230
Kzo9I	GATC	3 cut(s)	94, 192, 208
LmnI	GCTCC	1 cut(s)	243
LpnPI	CCDG	4 cut(s)	36, 41, 246, 273
Lsp1109I	GCAGC	1 cut(s)	214
LweI	GCATC	4 cut(s)	104, 168, 313, 321
MaeII	ACGT	1 cut(s)	339
MaeIII	GTNAC	1 cut(s)	266
MalI	GATC	3 cut(s)	96, 194, 210
MboI	GATC	3 cut(s)	94, 192, 208
MboII	GAAGA	3 cut(s)	124, 319, 332
MflI	RGATCY	1 cut(s)	208
MluCI	AATT	3 cut(s)	181, 243, 251
MmeI	TCCRAC	1 cut(s)	59
MnlI	CCTC	4 cut(s)	77, 89, 280, 292
MspR9I	CCNGG	1 cut(s)	261
MvaI	CCWGG	1 cut(s)	261
MvnI	CGCG	1 cut(s)	232
MwoI	GCNNNNNNNGC	2 cut(s)	72, 123
NdeI	CATATG	1 cut(s)	314
NdeII	GATC	3 cut(s)	94, 192, 208
NlaIII	CATG	1 cut(s)	113
NlaIV	GGNNCC	3 cut(s)	21, 210, 239
PcsI	WCGNNNNNNNCGW	2 cut(s)	141, 150
PfeI	GAWTC	1 cut(s)	31
PkrI	GCNGC	2 cut(s)	77, 229
Ppu21I	YACGTR	1 cut(s)	340
Psp6I	CCWGG	1 cut(s)	259
PspGI	CCWGG	1 cut(s)	259
PspN4I	GGNNCC	3 cut(s)	21, 210, 239
PsuI	RGATCY	1 cut(s)	208
RsaI	GTAC	1 cut(s)	342
RsaNI	GTAC	1 cut(s)	341
SatI	GCNGC	2 cut(s)	76, 228
Sau3AI	GATC	3 cut(s)	94, 192, 208
ScrFI	CCNGG	1 cut(s)	261
SetI	ASST	4 cut(s)	44, 57, 92, 342
SfaNI	GCATC	4 cut(s)	104, 168, 313, 321
SnaBI	TACGTA	1 cut(s)	340
Sse9I	AATT	3 cut(s)	181, 243, 251
SsiI	CCGC	3 cut(s)	76, 232, 344
StyD4I	CCNGG	1 cut(s)	259
TaiI	ACGT	1 cut(s)	342
TaqI	TCGA	1 cut(s)	135
TasI	AATT	3 cut(s)	181, 243, 251
TauI	GCSGC	1 cut(s)	78
TfiI	GAWTC	1 cut(s)	31
TseI	GCWGC	1 cut(s)	227
TspDTI	ATGAA	2 cut(s)	155, 320
TspGWI	ACGGA	1 cut(s)	59
XapI	RAATTY	1 cut(s)	251

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.