MD09G1286700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014512
Physical Location & Seq
Reverse (-)
36522864 .. 36524366
1503 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1286700.v1.1.491

Sequence Viewer

Length: 768 bp
ATGCAAACCCTCATTGCCACGGGCTCTTGCTGTCTTGGCAAGTTCCCTAACAACAATGCCAATTGGAAGCATCTTTTACCGAGTTCTCGTAGGCCTTCCTGCAACCTTAGAAATCAAACCACAACAAGAAACAGCAGCCTCAAAACCACTTGGCCTTCCATCTCGTTCGCACTTTTTGCTTCGGGTTTCTTCCTCGGTCCGTTGATTGATGGACTCCATTCAAGGGTTGATCTTGTGGTTTACCAAAACGGTTCAACCAATATCGGTCCTCTGCACACAAACGTCTGGGTTCCCGTCTTGCTCGGTCTGTTCTACTGCACTGTTGGCATGTTTCAACTAGTCTTGGATGAAAGGACCTCTTCAAAGGTTCAGCAAGTGAACCAAGAGAAAGCGTTTGCTTCTTTGTTATCACTTGTGCTGTTTATAGAGTTGAGCGCCGAACTGTACAAAGCAGGAATCGCAGACAATACTGAAGCCTACATATTATTTGCAGGAGCTGAGTTTCTATGGTTTTTCCTTGACAGAACATGGTTCGGCTTCCTCCTCGCAAGTGTCATCGGCCTTGGCTGCCCGCTGGCCGAGATTCCGTTAATGCAGTTCTTCCACCTCTGGTTTTATCCACAAGCAAACGTTGAGATCTTCGGCCAGGGACTAGTGAGTTGGACACTCACTTGCTATTTTGTTTACACGCTGTTTTTGATCAATTTCTCACGTTGGCTGAAATCAGTGATCGTTGATGCTGCCGATACGGAGCAGAAGTCCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
INSIG

Protein Analysis

256

Amino Acids

28.65

Weight (kDa)

6.88

Isoelectric Point (pI)

34.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
INSIG PF07281 56 - 211 6.2e-07 Insulin-induced protein (INSIG)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014512)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01935
fragaria_vesca FvH4_1g26780
malus_domestica MD09G1286700.v1.1
prunus_persica Prupe.3G006900_v2.0.a1
pyrus_communis pycom09g19380
rosa_chinensis RchiOBHm_Chr3g0487001
rosa_laevigata RLG00000023040
rosa_multiflora Rmu_sc0005856.1_g000001 Rmu_sc0009825.1_g000004 Rmu_sc0027023.1_g000002
rosa_roxburghii Rroxscaffold_6G00395260
rosa_rugosa Rorug03G0229100
rosa_samantha Rh3AG278600 Rh3BG314400 Rh3CG312800 Rh3DG309300
rosa_wichuraiana Rw0G001680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 572
AclI AACGTT 1 cut(s) 630
AcoI YGGCCR 2 cut(s) 576, 643
AcuI CTGAAG 1 cut(s) 492
AfaI GTAC 1 cut(s) 446
AfiI CCNNNNNNNGG 1 cut(s) 223
AgsI TTSAA 4 cut(s) 222, 255, 335, 363
AhlI ACTAGT 2 cut(s) 337, 652
AjnI CCWGG 1 cut(s) 645
AluBI AGCT 1 cut(s) 497
AluI AGCT 1 cut(s) 497
AlwNI CAGNNNCTG 1 cut(s) 497
AoxI GGCC 5 cut(s) 92, 152, 559, 576, 643
ApeKI GCWGC 3 cut(s) 135, 567, 740
AspLEI GCGC 1 cut(s) 437
AspS9I GGNCC 3 cut(s) 197, 266, 354
AvaII GGWCC 3 cut(s) 197, 266, 354
BanII GRGCYC 1 cut(s) 26
BbvI GCAGC 3 cut(s) 147, 554, 727
BccI CCATC 2 cut(s) 167, 203
BciT130I CCWGG 1 cut(s) 647
BclI TGATCA 1 cut(s) 699
BcuI ACTAGT 2 cut(s) 337, 652
BfaI CTAG 2 cut(s) 338, 653
BfoI RGCGCY 1 cut(s) 438
BglII AGATCT 1 cut(s) 636
BisI GCNGC 3 cut(s) 136, 568, 741
BlsI GCNGC 3 cut(s) 137, 569, 742
Bme1390I CCNGG 1 cut(s) 647
Bme18I GGWCC 3 cut(s) 197, 266, 354
BmgT120I GGNCC 3 cut(s) 197, 266, 354
BmiI GGNNCC 1 cut(s) 291
BmrFI CCNGG 1 cut(s) 647
BmsI GCATC 2 cut(s) 79, 727
BsaJI CCNNGG 4 cut(s) 18, 193, 562, 646
BsaXI ACNNNNNCTCC 1 cut(s) 743
Bsc4I CCNNNNNNNGG 1 cut(s) 223
Bse3DI GCAATG 1 cut(s) 12
BseBI CCWGG 1 cut(s) 647
BseDI CCNNGG 4 cut(s) 18, 193, 562, 646
BseGI GGATG 1 cut(s) 352
BseLI CCNNNNNNNGG 1 cut(s) 223
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 1 cut(s) 489
BseRI GAGGAG 1 cut(s) 533
BseXI GCAGC 3 cut(s) 147, 554, 727
BsgI GTGCAG 2 cut(s) 257, 301
BshFI GGCC 5 cut(s) 94, 154, 561, 578, 645
BslFI GGGAC 1 cut(s) 663
BslI CCNNNNNNNGG 1 cut(s) 223
BsmFI GGGAC 1 cut(s) 663
BsnI GGCC 5 cut(s) 94, 154, 561, 578, 645
Bsp1286I GDGCHC 1 cut(s) 26
Bsp1407I TGTACA 1 cut(s) 444
Bsp143I GATC 4 cut(s) 229, 636, 699, 729
BspACI CCGC 1 cut(s) 572
BspANI GGCC 5 cut(s) 94, 154, 561, 578, 645
BspCNI CTCAG 1 cut(s) 490
BspLI GGNNCC 1 cut(s) 291
BsrDI GCAATG 1 cut(s) 12
BsrGI TGTACA 1 cut(s) 444
BssECI CCNNGG 4 cut(s) 18, 193, 562, 646
BssMI GATC 4 cut(s) 229, 636, 699, 729
BssT1I CCWWGG 1 cut(s) 562
Bst2UI CCWGG 1 cut(s) 647
Bst4CI ACNGT 3 cut(s) 251, 322, 444
Bst6I CTCTTC 1 cut(s) 364
BstAPI GCANNNNNTGC 1 cut(s) 176
BstAUI TGTACA 1 cut(s) 444
BstC8I GCNNGC 2 cut(s) 572, 576
BstDEI CTNAG 2 cut(s) 107, 498
BstDSI CCRYGG 1 cut(s) 18
BstF5I GGATG 1 cut(s) 352
BstH2I RGCGCY 1 cut(s) 438
BstHHI GCGC 1 cut(s) 437
BstKTI GATC 4 cut(s) 232, 639, 702, 732
BstMBI GATC 4 cut(s) 229, 636, 699, 729
BstMWI GCNNNNNNNGC 5 cut(s) 36, 176, 324, 458, 567
BstNI CCWGG 1 cut(s) 647
BstNSI RCATGY 1 cut(s) 331
BstSCI CCNGG 1 cut(s) 645
BstV1I GCAGC 3 cut(s) 147, 554, 727
BstX2I RGATCY 1 cut(s) 636
BstYI RGATCY 1 cut(s) 636
BsuRI GGCC 5 cut(s) 94, 154, 561, 578, 645
BtgI CCRYGG 1 cut(s) 18
BtsCI GGATG 1 cut(s) 352
BtsIMutI CAGTG 2 cut(s) 318, 732
Cac8I GCNNGC 2 cut(s) 572, 576
CaiI CAGNNNCTG 1 cut(s) 497
CfoI GCGC 1 cut(s) 437
Cfr13I GGNCC 3 cut(s) 197, 266, 354
CpoI CGGWCCG 1 cut(s) 197
Csp6I GTAC 1 cut(s) 445
CspI CGGWCCG 1 cut(s) 197
CviAII CATG 2 cut(s) 328, 528
CviQI GTAC 1 cut(s) 445
DdeI CTNAG 2 cut(s) 107, 498
DpnI GATC 4 cut(s) 231, 638, 701, 731
DpnII GATC 4 cut(s) 229, 636, 699, 729
EaeI YGGCCR 2 cut(s) 576, 643
Eam1104I CTCTTC 1 cut(s) 364
EarI CTCTTC 1 cut(s) 364
Eco130I CCWWGG 1 cut(s) 562
Eco147I AGGCCT 1 cut(s) 94
Eco24I GRGCYC 1 cut(s) 26
Eco47I GGWCC 3 cut(s) 197, 266, 354
Eco57I CTGAAG 1 cut(s) 492
EcoO109I RGGNCCY 1 cut(s) 354
EcoRII CCWGG 1 cut(s) 645
EcoT14I CCWWGG 1 cut(s) 562
EcoT38I GRGCYC 1 cut(s) 26
ErhI CCWWGG 1 cut(s) 562
FaeI CATG 2 cut(s) 331, 531
FaiI YATR 5 cut(s) 329, 425, 482, 508, 529
FalI AAGNNNNNCTT 2 cut(s) 343, 375
FaqI GGGAC 1 cut(s) 663
FatI CATG 2 cut(s) 327, 527
FauI CCCGC 1 cut(s) 579
FbaI TGATCA 1 cut(s) 699
Fnu4HI GCNGC 3 cut(s) 136, 568, 741
FokI GGATG 1 cut(s) 359
FriOI GRGCYC 1 cut(s) 26
Fsp4HI GCNGC 3 cut(s) 136, 568, 741
FspBI CTAG 2 cut(s) 338, 653
GlaI GCGC 1 cut(s) 436
GluI GCNGC 3 cut(s) 136, 568, 741
HaeII RGCGCY 1 cut(s) 438
HaeIII GGCC 5 cut(s) 94, 154, 561, 578, 645
HhaI GCGC 1 cut(s) 437
Hin1II CATG 2 cut(s) 331, 531
Hin6I GCGC 1 cut(s) 435
HinP1I GCGC 1 cut(s) 435
HinfI GANTC 3 cut(s) 213, 456, 583
Hpy166II GTNNAC 3 cut(s) 241, 379, 685
Hpy8I GTNNAC 3 cut(s) 241, 379, 685
HpyAV CCTTC 2 cut(s) 105, 165
HpyCH4III ACNGT 3 cut(s) 251, 322, 444
HpyCH4IV ACGT 3 cut(s) 282, 630, 712
HpyCH4V TGCA 6 cut(s) 4, 102, 274, 318, 491, 595
HpyF10VI GCNNNNNNNGC 5 cut(s) 36, 176, 324, 458, 567
HpyF3I CTNAG 2 cut(s) 107, 498
HpySE526I ACGT 3 cut(s) 282, 630, 712
Hsp92II CATG 2 cut(s) 331, 531
HspAI GCGC 1 cut(s) 435
Ksp22I TGATCA 1 cut(s) 699
Kzo9I GATC 4 cut(s) 229, 636, 699, 729
LmnI GCTCC 2 cut(s) 494, 751
LpnPI CCDG 8 cut(s) 112, 271, 438, 477, 560, 595, 632, 659
Lsp1109I GCAGC 3 cut(s) 147, 554, 727
LweI GCATC 2 cut(s) 79, 727
MaeI CTAG 2 cut(s) 338, 653
MaeII ACGT 3 cut(s) 282, 630, 712
MalI GATC 4 cut(s) 231, 638, 701, 731
MboI GATC 4 cut(s) 229, 636, 699, 729
MboII GAAGA 4 cut(s) 181, 351, 592, 631
MfeI CAATTG 1 cut(s) 61
MflI RGATCY 1 cut(s) 636
MhlI GDGCHC 1 cut(s) 26
MluCI AATT 2 cut(s) 61, 703
MlyI GAGTC 1 cut(s) 207
MmeI TCCRAC 1 cut(s) 641
MnlI CCTC 8 cut(s) 20, 149, 203, 279, 367, 551, 554, 617
MseI TTAA 2 cut(s) 590, 766
MspA1I CMGCKG 1 cut(s) 574
MspR9I CCNGG 1 cut(s) 647
MunI CAATTG 1 cut(s) 61
MvaI CCWGG 1 cut(s) 647
MwoI GCNNNNNNNGC 5 cut(s) 36, 176, 324, 458, 567
NdeII GATC 4 cut(s) 229, 636, 699, 729
NlaIII CATG 2 cut(s) 331, 531
NlaIV GGNNCC 1 cut(s) 291
NmeAIII GCCGAG 1 cut(s) 604
NspI RCATGY 1 cut(s) 331
PceI AGGCCT 1 cut(s) 94
PfeI GAWTC 2 cut(s) 456, 583
PkrI GCNGC 3 cut(s) 137, 569, 742
PleI GAGTC 1 cut(s) 207
PpsI GAGTC 1 cut(s) 207
PpuMI RGGWCCY 1 cut(s) 354
Psp1406I AACGTT 1 cut(s) 630
Psp5II RGGWCCY 1 cut(s) 354
Psp6I CCWGG 1 cut(s) 645
PspGI CCWGG 1 cut(s) 645
PspN4I GGNNCC 1 cut(s) 291
PspPI GGNCC 3 cut(s) 197, 266, 354
PspPPI RGGWCCY 1 cut(s) 354
PstNI CAGNNNCTG 1 cut(s) 497
PsuI RGATCY 1 cut(s) 636
RsaI GTAC 1 cut(s) 446
RsaNI GTAC 1 cut(s) 445
Rsr2I CGGWCCG 1 cut(s) 197
RsrII CGGWCCG 1 cut(s) 197
SaqAI TTAA 2 cut(s) 590, 766
SatI GCNGC 3 cut(s) 136, 568, 741
Sau3AI GATC 4 cut(s) 229, 636, 699, 729
Sau96I GGNCC 3 cut(s) 197, 266, 354
SchI GAGTC 1 cut(s) 207
ScrFI CCNGG 1 cut(s) 647
SduI GDGCHC 1 cut(s) 26
SetI ASST 8 cut(s) 108, 285, 359, 369, 499, 609, 633, 715
SfaNI GCATC 2 cut(s) 79, 727
SinI GGWCC 3 cut(s) 197, 266, 354
SpeI ACTAGT 2 cut(s) 337, 652
Sse9I AATT 2 cut(s) 61, 703
SseBI AGGCCT 1 cut(s) 94
SsiI CCGC 1 cut(s) 572
SspMI CTAG 2 cut(s) 338, 653
StuI AGGCCT 1 cut(s) 94
StyD4I CCNGG 1 cut(s) 645
StyI CCWWGG 1 cut(s) 562
TaaI ACNGT 3 cut(s) 251, 322, 444
TaiI ACGT 3 cut(s) 285, 633, 715
TaqII GACCGA 3 cut(s) 185, 254, 293
TasI AATT 2 cut(s) 61, 703
TatI WGTACW 1 cut(s) 444
TfiI GAWTC 2 cut(s) 456, 583
Tru1I TTAA 2 cut(s) 590, 766
Tru9I TTAA 2 cut(s) 590, 766
TscAI CASTG 2 cut(s) 325, 732
TseI GCWGC 3 cut(s) 135, 567, 740
TspDTI ATGAA 1 cut(s) 363
TspGWI ACGGA 3 cut(s) 189, 576, 764
TspRI CASTG 2 cut(s) 325, 732
VpaK11BI GGWCC 3 cut(s) 197, 266, 354
XceI RCATGY 1 cut(s) 331
XspI CTAG 2 cut(s) 338, 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.