RLG00000023040

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0014512
Physical Location & Seq
Reverse (-)
17104450 .. 17105755
1306 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023040

Sequence Viewer

Length: 783 bp
ATGCGAGCCCTCATTGGCACTGCTCCAGGCTCTATCTTTCTTCACAATTTCCCTAACAATGCCCGGTGCAAACAAGCTTTACTTATTAGGCGTAGGCCCTCCTGTTGCATTAGAAAGCAAAGCACACCAACAACCAGAAGCAGCAGCAGCAGCAGCCTCAAAACCACCTGGCCATCCCTCTCATTTTCACTTTTCGGTTCCGGTTTCGTCCTCGGTCCTCTGATTGATGGAATCCATTCAAGGGTAGAACTTGTGGTGTACCAAAATGGCTCAATCAATATTGGTCCTCTTCACACAAACGTCTGGGTTCCCGTCTTACTTGGTCTGTTCTACTGCACTGTCGGGTTGCTTCAACTCTTCTTGGATGAAAGGGCCTCCTTAAAGATTCCAGAGGGAAACCAACAGAAAATGCTTGCTTCTTTATTAGCACTCGTGCTATTTATAGAGTTGAGCGCTGAGATGTATAAAGCAGGCGTCGCAGACAATATAGAAGCATACATACTATTTGCAGCGGCTGAGTTTATATGGTTTTCTGTGGATCGGACATGGTTTGGCTTCCTCCTCGCAAGCATAGTTGGTCTGGCCTGCCCGGTGGCTGAGATACCCTTAATGAAGTTCTTCAACCTCTGGTATTATCCACAAGCGAATGTCGAAATCTCTGGCCAGGGCCTGGTGACTTGGACACTCACTTGCTATTTTGTTTACACGCTGTTTCTGATCAATTTTTCACGCTGGCTGAAATCAGTGATCAGTTCTACTAATACGGAGAACAAGTCCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
INSIG

Protein Analysis

261

Amino Acids

28.98

Weight (kDa)

9.07

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
INSIG PF07281 62 - 219 1.1e-07 Insulin-induced protein (INSIG)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014512)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01935
fragaria_vesca FvH4_1g26780
malus_domestica MD09G1286700.v1.1
prunus_persica Prupe.3G006900_v2.0.a1
pyrus_communis pycom09g19380
rosa_chinensis RchiOBHm_Chr3g0487001
rosa_laevigata RLG00000023040
rosa_multiflora Rmu_sc0005856.1_g000001 Rmu_sc0009825.1_g000004 Rmu_sc0027023.1_g000002
rosa_roxburghii Rroxscaffold_6G00395260
rosa_rugosa Rorug03G0229100
rosa_samantha Rh3AG278600 Rh3BG314400 Rh3CG312800 Rh3DG309300
rosa_wichuraiana Rw0G001680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 670
AciI CCGC 1 cut(s) 512
AclWI GGATC 1 cut(s) 546
AcoI YGGCCR 2 cut(s) 170, 661
AcyI GRCGYC 1 cut(s) 474
AfaI GTAC 1 cut(s) 260
AfeI AGCGCT 1 cut(s) 454
AfiI CCNNNNNNNGG 2 cut(s) 241, 670
AgsI TTSAA 3 cut(s) 240, 353, 622
AjnI CCWGG 4 cut(s) 25, 167, 663, 669
AluBI AGCT 1 cut(s) 77
AluI AGCT 1 cut(s) 77
AlwI GGATC 1 cut(s) 546
AlwNI CAGNNNCTG 2 cut(s) 515, 670
Aor51HI AGCGCT 1 cut(s) 454
AoxI GGCC 6 cut(s) 95, 170, 372, 582, 661, 667
ApeKI GCWGC 6 cut(s) 141, 144, 147, 150, 153, 509
Asp700I GAANNNNTTC 2 cut(s) 235, 617
AspLEI GCGC 1 cut(s) 455
AspS9I GGNCC 5 cut(s) 96, 215, 284, 372, 667
AsuC2I CCSGG 2 cut(s) 64, 590
AsuHPI GGTGA 1 cut(s) 685
AvaII GGWCC 2 cut(s) 215, 284
BalI TGGCCA 2 cut(s) 172, 663
BanII GRGCYC 1 cut(s) 10
BarI GAAGNNNNNNTAC 2 cut(s) 483, 515
BauI CACGAG 1 cut(s) 431
BbvI GCAGC 6 cut(s) 153, 156, 159, 162, 165, 521
BccI CCATC 2 cut(s) 181, 221
BciT130I CCWGG 4 cut(s) 27, 169, 665, 671
BclI TGATCA 2 cut(s) 717, 747
BcnI CCSGG 2 cut(s) 64, 590
BfoI RGCGCY 1 cut(s) 456
BisI GCNGC 7 cut(s) 142, 145, 148, 151, 154, 510, 513
BlsI GCNGC 7 cut(s) 143, 146, 149, 152, 155, 511, 514
Bme1390I CCNGG 6 cut(s) 27, 64, 169, 590, 665, 671
Bme18I GGWCC 2 cut(s) 215, 284
BmgT120I GGNCC 5 cut(s) 96, 215, 284, 372, 667
BmiI GGNNCC 2 cut(s) 199, 309
BmrFI CCNGG 6 cut(s) 27, 64, 169, 590, 665, 671
BpmI CTGGAG 1 cut(s) 9
BpuMI CCSGG 2 cut(s) 64, 590
BsaHI GRCGYC 1 cut(s) 474
BsaJI CCNNGG 2 cut(s) 211, 664
BsaWI WCCGGW 1 cut(s) 200
BsaXI ACNNNNNCTCC 1 cut(s) 758
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 670
BseBI CCWGG 4 cut(s) 27, 169, 665, 671
BseDI CCNNGG 2 cut(s) 211, 664
BseGI GGATG 2 cut(s) 173, 370
BseLI CCNNNNNNNGG 2 cut(s) 241, 670
BseMII CTCAG 3 cut(s) 447, 507, 588
BseRI GAGGAG 1 cut(s) 551
BseXI GCAGC 6 cut(s) 153, 156, 159, 162, 165, 521
BsgI GTGCAG 1 cut(s) 319
BshFI GGCC 6 cut(s) 97, 172, 374, 584, 663, 669
BsiSI CCGG 3 cut(s) 64, 201, 590
BslI CCNNNNNNNGG 2 cut(s) 241, 670
BsnI GGCC 6 cut(s) 97, 172, 374, 584, 663, 669
Bsp1286I GDGCHC 1 cut(s) 10
Bsp143I GATC 3 cut(s) 538, 717, 747
BspACI CCGC 1 cut(s) 512
BspANI GGCC 6 cut(s) 97, 172, 374, 584, 663, 669
BspCNI CTCAG 3 cut(s) 448, 508, 589
BspLI GGNNCC 2 cut(s) 199, 309
BspPI GGATC 1 cut(s) 546
BssECI CCNNGG 2 cut(s) 211, 664
BssMI GATC 3 cut(s) 538, 717, 747
BssNI GRCGYC 1 cut(s) 474
BssSI CACGAG 1 cut(s) 431
Bst2BI CACGAG 1 cut(s) 431
Bst2UI CCWGG 4 cut(s) 27, 169, 665, 671
Bst4CI ACNGT 1 cut(s) 340
Bst6I CTCTTC 2 cut(s) 294, 362
BstACI GRCGYC 1 cut(s) 474
BstC8I GCNNGC 6 cut(s) 6, 414, 472, 568, 586, 734
BstDEI CTNAG 4 cut(s) 456, 516, 597, 780
BstF5I GGATG 2 cut(s) 173, 370
BstH2I RGCGCY 1 cut(s) 456
BstHHI GCGC 1 cut(s) 455
BstKTI GATC 3 cut(s) 541, 720, 750
BstMBI GATC 3 cut(s) 538, 717, 747
BstMWI GCNNNNNNNGC 4 cut(s) 147, 150, 153, 476
BstNI CCWGG 4 cut(s) 27, 169, 665, 671
BstSCI CCNGG 6 cut(s) 25, 62, 167, 588, 663, 669
BstV1I GCAGC 6 cut(s) 153, 156, 159, 162, 165, 521
BsuRI GGCC 6 cut(s) 97, 172, 374, 584, 663, 669
BtsCI GGATG 2 cut(s) 173, 370
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 3 cut(s) 18, 336, 750
Cac8I GCNNGC 6 cut(s) 6, 414, 472, 568, 586, 734
CaiI CAGNNNCTG 2 cut(s) 515, 670
CfoI GCGC 1 cut(s) 455
Cfr13I GGNCC 5 cut(s) 96, 215, 284, 372, 667
CseI GACGC 1 cut(s) 463
Csp6I GTAC 1 cut(s) 259
CviAII CATG 1 cut(s) 546
CviQI GTAC 1 cut(s) 259
DdeI CTNAG 4 cut(s) 456, 516, 597, 780
DpnI GATC 3 cut(s) 540, 719, 749
DpnII GATC 3 cut(s) 538, 717, 747
EaeI YGGCCR 2 cut(s) 170, 661
Eam1104I CTCTTC 2 cut(s) 294, 362
EarI CTCTTC 2 cut(s) 294, 362
Eco24I GRGCYC 1 cut(s) 10
Eco47I GGWCC 2 cut(s) 215, 284
Eco47III AGCGCT 1 cut(s) 454
EcoO109I RGGNCCY 3 cut(s) 96, 372, 667
EcoRII CCWGG 4 cut(s) 25, 167, 663, 669
EcoT38I GRGCYC 1 cut(s) 10
FaeI CATG 1 cut(s) 549
FaiI YATR 9 cut(s) 443, 465, 488, 496, 500, 524, 526, 547, 572
FalI AAGNNNNNCTT 2 cut(s) 66, 98
FatI CATG 1 cut(s) 545
FbaI TGATCA 2 cut(s) 717, 747
Fnu4HI GCNGC 7 cut(s) 142, 145, 148, 151, 154, 510, 513
FokI GGATG 2 cut(s) 160, 377
FriOI GRGCYC 1 cut(s) 10
Fsp4HI GCNGC 7 cut(s) 142, 145, 148, 151, 154, 510, 513
GlaI GCGC 1 cut(s) 454
GluI GCNGC 7 cut(s) 142, 145, 148, 151, 154, 510, 513
GsuI CTGGAG 1 cut(s) 9
HaeII RGCGCY 1 cut(s) 456
HaeIII GGCC 6 cut(s) 97, 172, 374, 584, 663, 669
HapII CCGG 3 cut(s) 64, 201, 590
HgaI GACGC 1 cut(s) 463
HhaI GCGC 1 cut(s) 455
Hin1I GRCGYC 1 cut(s) 474
Hin1II CATG 1 cut(s) 549
Hin6I GCGC 1 cut(s) 453
HinP1I GCGC 1 cut(s) 453
HindIII AAGCTT 1 cut(s) 75
HinfI GANTC 2 cut(s) 231, 385
HpaII CCGG 3 cut(s) 64, 201, 590
HphI GGTGA 1 cut(s) 685
Hpy166II GTNNAC 2 cut(s) 259, 703
Hpy188I TCNGA 3 cut(s) 222, 543, 717
Hpy188III TCNNGA 1 cut(s) 389
Hpy8I GTNNAC 2 cut(s) 259, 703
Hpy99I CGWCG 1 cut(s) 479
HpyCH4III ACNGT 1 cut(s) 340
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 4 cut(s) 69, 108, 336, 509
HpyF10VI GCNNNNNNNGC 4 cut(s) 147, 150, 153, 476
HpyF3I CTNAG 4 cut(s) 456, 516, 597, 780
HpySE526I ACGT 1 cut(s) 300
Hsp92I GRCGYC 1 cut(s) 474
Hsp92II CATG 1 cut(s) 549
HspAI GCGC 1 cut(s) 453
Ksp22I TGATCA 2 cut(s) 717, 747
Kzo9I GATC 3 cut(s) 538, 717, 747
LmnI GCTCC 1 cut(s) 28
Lsp1109I GCAGC 6 cut(s) 153, 156, 159, 162, 165, 521
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 1 cut(s) 673
MalI GATC 3 cut(s) 540, 719, 749
MboI GATC 3 cut(s) 538, 717, 747
MboII GAAGA 4 cut(s) 32, 281, 349, 610
MhlI GDGCHC 1 cut(s) 10
MlsI TGGCCA 2 cut(s) 172, 663
MluCI AATT 2 cut(s) 46, 721
MluNI TGGCCA 2 cut(s) 172, 663
Mox20I TGGCCA 2 cut(s) 172, 663
MroXI GAANNNNTTC 2 cut(s) 235, 617
MscI TGGCCA 2 cut(s) 172, 663
MseI TTAA 2 cut(s) 380, 608
Msp20I TGGCCA 2 cut(s) 172, 663
MspA1I CMGCKG 1 cut(s) 512
MspI CCGG 3 cut(s) 64, 201, 590
MspR9I CCNGG 6 cut(s) 27, 64, 169, 590, 665, 671
MvaI CCWGG 4 cut(s) 27, 169, 665, 671
MwoI GCNNNNNNNGC 4 cut(s) 147, 150, 153, 476
NciI CCSGG 2 cut(s) 64, 590
NdeII GATC 3 cut(s) 538, 717, 747
NlaIII CATG 1 cut(s) 549
NlaIV GGNNCC 2 cut(s) 199, 309
NmuCI GTSAC 1 cut(s) 673
PdmI GAANNNNTTC 2 cut(s) 235, 617
PfeI GAWTC 2 cut(s) 231, 385
PflMI CCANNNNNTGG 1 cut(s) 670
PkrI GCNGC 7 cut(s) 143, 146, 149, 152, 155, 511, 514
Psp6I CCWGG 4 cut(s) 25, 167, 663, 669
PspGI CCWGG 4 cut(s) 25, 167, 663, 669
PspN4I GGNNCC 2 cut(s) 199, 309
PspPI GGNCC 5 cut(s) 96, 215, 284, 372, 667
PstNI CAGNNNCTG 2 cut(s) 515, 670
RsaI GTAC 1 cut(s) 260
RsaNI GTAC 1 cut(s) 259
SaqAI TTAA 2 cut(s) 380, 608
SatI GCNGC 7 cut(s) 142, 145, 148, 151, 154, 510, 513
Sau3AI GATC 3 cut(s) 538, 717, 747
Sau96I GGNCC 5 cut(s) 96, 215, 284, 372, 667
ScrFI CCNGG 6 cut(s) 27, 64, 169, 590, 665, 671
SduI GDGCHC 1 cut(s) 10
SetI ASST 4 cut(s) 79, 170, 303, 627
SinI GGWCC 2 cut(s) 215, 284
Sse9I AATT 2 cut(s) 46, 721
SsiI CCGC 1 cut(s) 512
SspI AATATT 1 cut(s) 280
StyD4I CCNGG 6 cut(s) 25, 62, 167, 588, 663, 669
TaaI ACNGT 1 cut(s) 340
TaiI ACGT 1 cut(s) 303
TaqI TCGA 1 cut(s) 651
TaqII GACCGA 1 cut(s) 203
TasI AATT 2 cut(s) 46, 721
TauI GCSGC 1 cut(s) 515
TfiI GAWTC 2 cut(s) 231, 385
Tru1I TTAA 2 cut(s) 380, 608
Tru9I TTAA 2 cut(s) 380, 608
TscAI CASTG 3 cut(s) 25, 343, 750
TseFI GTSAC 1 cut(s) 673
TseI GCWGC 6 cut(s) 141, 144, 147, 150, 153, 509
Tsp45I GTSAC 1 cut(s) 673
TspDTI ATGAA 2 cut(s) 381, 626
TspGWI ACGGA 1 cut(s) 779
TspRI CASTG 3 cut(s) 25, 343, 750
Van91I CCANNNNNTGG 1 cut(s) 670
VpaK11BI GGWCC 2 cut(s) 215, 284
XmnI GAANNNNTTC 2 cut(s) 235, 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.