Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0004750

Physical Location & Seq

Genomic Coordinates Reverse (-)

37439971 .. 37452006

Gene Structure 12036 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1292000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1860 bp

ATGTCGATACCAAAGGAGCCGGAGCAGGTGATGAAACTAAGAGGAGGATCAGTGCTGGGCAAGAAGACCATTCTCAAGAGCGACCACTTCCCTGGCTGCCAGAACAAACGCTTGTCTCCCCACATCGATGGTGCTCCCAATTACCGCCAGGCTGATTCGCAACATGTTCATGGTGTTGCCATCCAACAATCGGTGGAATCCAGAATGTTCTTAACCATATTGGAGCTCAACAAATTGATGGGAAGCGAGCACAAGTTCTTTGGATTAACCTTCGCGAGGAGCCGGGATTTAGTGCACTTATTAGTGTTGTGTGGTCTATATTATGGGCGCCCTTTTGTTTTGCATGATGTGGAGAGGCTCTTTTCCAACCTTGAATATACGGGAATTAACAGGGCTAGGGTTGAACAAATGGAAGCCTGGTTAAAGGAAGATATTTTAATTGAATCTGCAAGATATGGAAATAAGATCCTTCTCACTGATGAACTGCCAGATGGTCAGATGGTGGATCAATGGGAACCAGTGTCACGTCATTCTGTGAAGACACCACTAGAGGTTTACGAGGAATTGCAAGAACCAGGATACCTTGTTGACTATGAACGTGTACATAAAATTTCTCAAGCTGACATGAATGCAGAAATAATTTTTAACTGTCAAATGGGACGTGGGCGAACCACAACAGGAATGGTGATTGCAAATTTGATATACCTCAACCGTATTGGAGCTTCCATTCTAGAATTTGTTAACTTGATGGGTAGAGGAAGGACAACTACAGGTACGGTAATTGCTTGCCTTTTGAAACTCCAAATTGACTACGGGAGACCTATCAAAATCCTGGTTGACAATATTACCCTTGAAGAAGTGGATGGTGGTACCTCAAGTGGCGAAGAAACTGGAGGTAGTAGTTCTGCATCAACCTCCAGTGTTACAAATTTTAGAAATGAAAAGGAGCAAAGCCGTGTATTCGGCATGAATGACATCCTCTTGTTGTGGAAAATAACAAGATTATTTGATAATGGGGTGGAATGCCGGGAAGCCTTAGATGCTATAATTGACAGATGTTCTGCTCTACAGAACATACGTCAAGCTGTTCTGCAGTACAGAAAGGTATTCAGTCAACATGTTGAGCCAAGGGTAAGGAGGGTGGCATTAAATCGTGGTGCTGAGTACTTGGAGCGCTACTTCCGTTTAATTGCTTTTGCAGCATACTTGGGAAGTGAAGCATTTGATGAATTTTGTGGGCAAGGAGAATCTAGGATGACGTTTAAGAATTGGTTGCATCAGCGACCACAGGTTCAAGAAATGGAACATAAGAATAAGACCTGGACGCTTTTTAACTGTCCCTCTTACTCTAAGAGCTTAGAGGAGTTGAGAGCACCACTTGAGTCCCAGCATGGGGATGCTGTCTTGGAGGCCATTGTCAAGGCCCGTAGTGGTTCGGTTTTGGGGAAAGGTTCTATACTTAAAATGTATTTCTTTCCAGGTCAAAGGACGTCTAGTCACATACAAATACATGGTGCACCACATGTTTTTAAGCATGGCTACACCGACAATTCTTGGTGCCAAAGAGATGTTAGCATATTTAGGTGCCAAACCCAAAGCAGAAGGACTCAAAAAGTGATTTTAACTGATCTGAGAGAAGAAGCAGTTGTCTACATTAATGGCACACCATTTGTTCTACGGGATTTAAACAAACCTGTTGATACACTCAAGCATGTGGGAATCGCAGGCCCAGTGGTGGAACACATGGAAGCACGACTAAAAGAAGATATACTATCTGAGGTCAGGCGGTCTGGTGGCCATATGCTTTTACACCGTGAAGAATATAGCCCGGCTTTGAACCAGTCCAGTGTCAATACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PTPlike_phytase

Protein Analysis

620

Amino Acids

70.33

Weight (kDa)

7.98

Isoelectric Point (pI)

45.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PTPlike_phytase	PF14566	109 - 201	2.6e-18	Inositol hexakisphosphate
PTPlike_phytase	PF14566	206 - 234	8e-09	Inositol hexakisphosphate
PTPlike_phytase	PF14566	543 - 610	7.9e-18	Inositol hexakisphosphate

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004750)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G62010 AT3G62010
fragaria_vesca	FvH4_1g26030 FvH4_1g26030 FvH4_1g26030 FvH4_1g26030 FvH4_1g26030 FvH4_1g26030 FvH4_1g26030 FvH4_1g26030
malus_domestica	MD09G1291900.v1.1 MD09G1292000.v1.1 MD17G1285800.v1.1
prunus_persica	Prupe.3G000300_v2.0.a1 Prupe.3G000300_v2.0.a1 Prupe.3G000300_v2.0.a1
pyrus_communis	pycom09g20000 pycom17g28150 pycom17g28160
rosa_chinensis	RchiOBHm_Chr3g0485491
rosa_laevigata	RLG00000023148 RLG00000023149
rosa_multiflora	Rmu_sc0004711.1_g000027
rosa_roxburghii	Rroxscaffold_6G00397000
rosa_rugosa	Rorug03G0218500
rosa_samantha	Rh3AG268000 Rh3BG303500 Rh3CG301600 Rh3DG298400
rosa_wichuraiana	Rw3G023690 Rw3G023900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	16
AatII	GACGTC	1 cut(s)	1493
Acc36I	ACCTGC	1 cut(s)	16
Acc65I	GGTACC	1 cut(s)	869
AccB1I	GGYRCC	4 cut(s)	327, 869, 1557, 1584
AccI	GTMKAC	1 cut(s)	1650
AccII	CGCG	1 cut(s)	275
AciI	CCGC	2 cut(s)	145, 1786
AclWI	GGATC	3 cut(s)	55, 460, 513
AcoI	YGGCCR	1 cut(s)	1795
AcsI	RAATTY	5 cut(s)	609, 694, 734, 928, 1229
AcyI	GRCGYC	2 cut(s)	328, 1490
AfaI	GTAC	5 cut(s)	603, 775, 871, 1097, 1166
AfeI	AGCGCT	1 cut(s)	1175
AfiI	CCNNNNNNNGG	5 cut(s)	190, 276, 1392, 1393, 1735
AflIII	ACRYGT	4 cut(s)	163, 598, 1117, 1522
AgsI	TTSAA	7 cut(s)	374, 404, 443, 796, 854, 1295, 1837
AhdI	GACNNNNNGTC	1 cut(s)	1494
AjiI	CACGTC	2 cut(s)	527, 662
AjnI	CCWGG	7 cut(s)	91, 147, 416, 574, 831, 1319, 1477
AluBI	AGCT	5 cut(s)	226, 620, 722, 1085, 1356
AluI	AGCT	5 cut(s)	226, 620, 722, 1085, 1356
Alw21I	GWGCWC	6 cut(s)	136, 228, 252, 297, 1375, 1519
Alw26I	GTCTC	2 cut(s)	120, 811
Alw44I	GTGCAC	2 cut(s)	293, 1515
AlwI	GGATC	3 cut(s)	55, 460, 513
Aor51HI	AGCGCT	1 cut(s)	1175
AoxI	GGCC	4 cut(s)	1410, 1422, 1726, 1795
ApaLI	GTGCAC	2 cut(s)	293, 1515
ApeKI	GCWGC	2 cut(s)	96, 1199
ApoI	RAATTY	5 cut(s)	609, 694, 734, 928, 1229
AseI	ATTAAT	1 cut(s)	1656
Asp718I	GGTACC	1 cut(s)	869
AspLEI	GCGC	2 cut(s)	330, 1176
AspS9I	GGNCC	2 cut(s)	1423, 1727
AsuC2I	CCSGG	3 cut(s)	284, 1028, 1829
AsuHPI	GGTGA	2 cut(s)	40, 697
BaeGI	GKGCMC	2 cut(s)	297, 1519
BalI	TGGCCA	1 cut(s)	1797
BanI	GGYRCC	4 cut(s)	327, 869, 1557, 1584
BanII	GRGCYC	1 cut(s)	228
BarI	GAAGNNNNNNTAC	4 cut(s)	706, 738, 751, 783
BbsI	GAAGAC	2 cut(s)	71, 545
Bbv12I	GWGCWC	6 cut(s)	136, 228, 252, 297, 1375, 1519
BbvI	GCAGC	2 cut(s)	83, 1211
BccI	CCATC	7 cut(s)	122, 188, 232, 485, 493, 742, 857
BceAI	ACGGC	1 cut(s)	939
BciT130I	CCWGG	7 cut(s)	93, 149, 418, 576, 833, 1321, 1479
BciVI	GTATCC	1 cut(s)	572
BcnI	CCSGG	3 cut(s)	284, 1028, 1829
BcoDI	GTCTC	2 cut(s)	120, 811
BfaI	CTAG	5 cut(s)	396, 548, 731, 1251, 1494
BfmI	CTRYAG	3 cut(s)	768, 1067, 1091
BfoI	RGCGCY	2 cut(s)	331, 1177
BfuAI	ACCTGC	1 cut(s)	16
BfuI	GTATCC	1 cut(s)	572
BisI	GCNGC	2 cut(s)	97, 1200
BlsI	GCNGC	2 cut(s)	98, 1201
BmcAI	AGTACT	1 cut(s)	1166
BmeRI	GACNNNNNGTC	1 cut(s)	1494
BmgBI	CACGTC	2 cut(s)	527, 662
BmgT120I	GGNCC	2 cut(s)	1423, 1727
BmiI	GGNNCC	7 cut(s)	18, 281, 329, 516, 871, 1559, 1586
BmrI	ACTGGG	1 cut(s)	1724
BmsI	GCATC	4 cut(s)	917, 1030, 1285, 1387
BmuI	ACTGGG	1 cut(s)	1724
BpiI	GAAGAC	2 cut(s)	71, 545
BpmI	CTGGAG	2 cut(s)	901, 912
BpuEI	CTTGAG	5 cut(s)	59, 600, 859, 1400, 1691
BpuMI	CCSGG	3 cut(s)	284, 1028, 1829
Bsa29I	ATCGAT	1 cut(s)	126
BsaHI	GRCGYC	2 cut(s)	328, 1490
BsaI	GGTCTC	1 cut(s)	811
BsaJI	CCNNGG	2 cut(s)	91, 1127
BsaXI	ACNNNNNCTCC	2 cut(s)	36, 66
Bsc4I	CCNNNNNNNGG	5 cut(s)	190, 276, 1392, 1393, 1735
Bse1I	ACTGG	6 cut(s)	518, 895, 918, 1730, 1840, 1845
BseBI	CCWGG	7 cut(s)	93, 149, 418, 576, 833, 1321, 1479
BseCI	ATCGAT	1 cut(s)	126
BseDI	CCNNGG	2 cut(s)	91, 1127
BseGI	GGATG	5 cut(s)	180, 868, 975, 1260, 1402
BseLI	CCNNNNNNNGG	5 cut(s)	190, 276, 1392, 1393, 1735
BseMII	CTCAG	3 cut(s)	1152, 1622, 1767
BseNI	ACTGG	6 cut(s)	518, 895, 918, 1730, 1840, 1845
BseRI	GAGGAG	3 cut(s)	57, 292, 1376
BseSI	GKGCMC	2 cut(s)	297, 1519
BseXI	GCAGC	2 cut(s)	83, 1211
BseYI	CCCAGC	2 cut(s)	55, 1386
Bsh1236I	CGCG	1 cut(s)	275
BshFI	GGCC	4 cut(s)	1412, 1424, 1728, 1797
BshNI	GGYRCC	4 cut(s)	327, 869, 1557, 1584
BshVI	ATCGAT	1 cut(s)	126
BsiHKAI	GWGCWC	6 cut(s)	136, 228, 252, 297, 1375, 1519
BsiSI	CCGG	4 cut(s)	20, 283, 1027, 1829
BslFI	GGGAC	3 cut(s)	672, 1323, 1369
BslI	CCNNNNNNNGG	5 cut(s)	190, 276, 1392, 1393, 1735
BsmAI	GTCTC	2 cut(s)	120, 811
BsmFI	GGGAC	3 cut(s)	672, 1323, 1369
BsmI	GAATGC	2 cut(s)	634, 1028
BsnI	GGCC	4 cut(s)	1412, 1424, 1728, 1797
Bso31I	GGTCTC	1 cut(s)	811
Bsp1286I	GDGCHC	6 cut(s)	136, 228, 252, 297, 1375, 1519
Bsp1407I	TGTACA	1 cut(s)	601
Bsp143I	GATC	4 cut(s)	47, 465, 505, 1627
Bsp68I	TCGCGA	1 cut(s)	275
BspACI	CCGC	2 cut(s)	145, 1786
BspANI	GGCC	4 cut(s)	1412, 1424, 1728, 1797
BspCNI	CTCAG	3 cut(s)	1153, 1623, 1768
BspDI	ATCGAT	1 cut(s)	126
BspFNI	CGCG	1 cut(s)	275
BspLI	GGNNCC	7 cut(s)	18, 281, 329, 516, 871, 1559, 1586
BspMAI	CTGCAG	1 cut(s)	1095
BspMI	ACCTGC	1 cut(s)	16
BspPI	GGATC	3 cut(s)	55, 460, 513
BspT107I	GGYRCC	4 cut(s)	327, 869, 1557, 1584
BspTNI	GGTCTC	1 cut(s)	811
BsrGI	TGTACA	1 cut(s)	601
BsrI	ACTGG	6 cut(s)	518, 895, 918, 1730, 1840, 1845
BssECI	CCNNGG	2 cut(s)	91, 1127
BssMI	GATC	4 cut(s)	47, 465, 505, 1627
BssNI	GRCGYC	2 cut(s)	328, 1490
BssT1I	CCWWGG	1 cut(s)	1127
Bst2UI	CCWGG	7 cut(s)	93, 149, 418, 576, 833, 1321, 1479
Bst4CI	ACNGT	5 cut(s)	650, 713, 778, 1337, 1814
BstACI	GRCGYC	2 cut(s)	328, 1490
BstAUI	TGTACA	1 cut(s)	601
BstC8I	GCNNGC	3 cut(s)	248, 787, 1726
BstDEI	CTNAG	8 cut(s)	38, 1036, 1161, 1350, 1357, 1631, 1776, 1857
BstENI	CCTNNNNNAGG	1 cut(s)	274
BstF5I	GGATG	5 cut(s)	180, 868, 975, 1260, 1402
BstFNI	CGCG	1 cut(s)	275
BstH2I	RGCGCY	2 cut(s)	331, 1177
BstHHI	GCGC	2 cut(s)	330, 1176
BstKTI	GATC	4 cut(s)	50, 468, 508, 1630
BstMAI	GTCTC	2 cut(s)	120, 811
BstMBI	GATC	4 cut(s)	47, 465, 505, 1627
BstMWI	GCNNNNNNNGC	2 cut(s)	1040, 1199
BstNI	CCWGG	7 cut(s)	93, 149, 418, 576, 833, 1321, 1479
BstNSI	RCATGY	4 cut(s)	167, 1121, 1526, 1715
BstSFI	CTRYAG	3 cut(s)	768, 1067, 1091
BstSLI	GKGCMC	2 cut(s)	297, 1519
BstUI	CGCG	1 cut(s)	275
BstV1I	GCAGC	2 cut(s)	83, 1211
BstV2I	GAAGAC	2 cut(s)	71, 545
BstX2I	RGATCY	1 cut(s)	465
BstXI	CCANNNNNNTGG	2 cut(s)	92, 128
BstYI	RGATCY	1 cut(s)	465
Bsu15I	ATCGAT	1 cut(s)	126
BsuI	GTATCC	1 cut(s)	572
BsuRI	GGCC	4 cut(s)	1412, 1424, 1728, 1797
BsuTUI	ATCGAT	1 cut(s)	126
BtrI	CACGTC	2 cut(s)	527, 662
BtsCI	GGATG	5 cut(s)	180, 868, 975, 1260, 1402
BtsIMutI	CAGTG	6 cut(s)	57, 474, 525, 925, 1737, 1852
BtuMI	TCGCGA	1 cut(s)	275
BveI	ACCTGC	1 cut(s)	16
Cac8I	GCNNGC	3 cut(s)	248, 787, 1726
CfoI	GCGC	2 cut(s)	330, 1176
Cfr13I	GGNCC	2 cut(s)	1423, 1727
ClaI	ATCGAT	1 cut(s)	126
CseI	GACGC	1 cut(s)	1333
Csp6I	GTAC	5 cut(s)	602, 774, 870, 1096, 1165
CviQI	GTAC	5 cut(s)	602, 774, 870, 1096, 1165
DdeI	CTNAG	8 cut(s)	38, 1036, 1161, 1350, 1357, 1631, 1776, 1857
DinI	GGCGCC	1 cut(s)	329
DpnI	GATC	4 cut(s)	49, 467, 507, 1629
DpnII	GATC	4 cut(s)	47, 465, 505, 1627
DraI	TTTAAA	1 cut(s)	1686
DriI	GACNNNNNGTC	1 cut(s)	1494
EaeI	YGGCCR	1 cut(s)	1795
Eam1105I	GACNNNNNGTC	1 cut(s)	1494
Ecl136II	GAGCTC	1 cut(s)	226
Eco130I	CCWWGG	1 cut(s)	1127
Eco24I	GRGCYC	1 cut(s)	228
Eco31I	GGTCTC	1 cut(s)	811
Eco47III	AGCGCT	1 cut(s)	1175
Eco53kI	GAGCTC	1 cut(s)	226
EcoICRI	GAGCTC	1 cut(s)	226
EcoNI	CCTNNNNNAGG	1 cut(s)	274
EcoRII	CCWGG	7 cut(s)	91, 147, 416, 574, 831, 1319, 1477
EcoT14I	CCWWGG	1 cut(s)	1127
EcoT38I	GRGCYC	1 cut(s)	228
EgeI	GGCGCC	1 cut(s)	329
EheI	GGCGCC	1 cut(s)	329
ErhI	CCWWGG	1 cut(s)	1127
FaqI	GGGAC	3 cut(s)	672, 1323, 1369
FauNDI	CATATG	1 cut(s)	1800
FblI	GTMKAC	1 cut(s)	1650
Fnu4HI	GCNGC	2 cut(s)	97, 1200
FokI	GGATG	5 cut(s)	167, 875, 962, 1267, 1409
FriOI	GRGCYC	1 cut(s)	228
Fsp4HI	GCNGC	2 cut(s)	97, 1200
FspBI	CTAG	5 cut(s)	396, 548, 731, 1251, 1494
GlaI	GCGC	2 cut(s)	329, 1175
GluI	GCNGC	2 cut(s)	97, 1200
GsaI	CCCAGC	2 cut(s)	59, 1390
GsuI	CTGGAG	2 cut(s)	901, 912
HaeII	RGCGCY	2 cut(s)	331, 1177
HaeIII	GGCC	4 cut(s)	1412, 1424, 1728, 1797
HapII	CCGG	4 cut(s)	20, 283, 1027, 1829
HgaI	GACGC	1 cut(s)	1333
HhaI	GCGC	2 cut(s)	330, 1176
Hin1I	GRCGYC	2 cut(s)	328, 1490
Hin6I	GCGC	2 cut(s)	328, 1174
HinP1I	GCGC	2 cut(s)	328, 1174
HincII	GTYRAC	4 cut(s)	589, 742, 838, 1115
HindII	GTYRAC	4 cut(s)	589, 742, 838, 1115
HinfI	GANTC	7 cut(s)	155, 197, 443, 1247, 1382, 1606, 1719
HpaI	GTTAAC	1 cut(s)	742
HpaII	CCGG	4 cut(s)	20, 283, 1027, 1829
HphI	GGTGA	2 cut(s)	40, 697
Hpy166II	GTNNAC	9 cut(s)	295, 556, 589, 602, 742, 838, 1115, 1517, 1651
Hpy188I	TCNGA	3 cut(s)	498, 1632, 1777
Hpy188III	TCNNGA	5 cut(s)	76, 201, 274, 731, 1295
Hpy8I	GTNNAC	9 cut(s)	295, 556, 589, 602, 742, 838, 1115, 1517, 1651
HpyAV	CCTTC	4 cut(s)	280, 479, 753, 1596
HpyCH4III	ACNGT	5 cut(s)	650, 713, 778, 1337, 1814
HpyCH4IV	ACGT	6 cut(s)	526, 598, 661, 1078, 1259, 1490
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1040, 1199
HpyF3I	CTNAG	8 cut(s)	38, 1036, 1161, 1350, 1357, 1631, 1776, 1857
HpySE526I	ACGT	6 cut(s)	526, 598, 661, 1078, 1259, 1490
Hsp92I	GRCGYC	2 cut(s)	328, 1490
HspAI	GCGC	2 cut(s)	328, 1174
KasI	GGCGCC	1 cut(s)	327
KpnI	GGTACC	1 cut(s)	873
KspAI	GTTAAC	1 cut(s)	742
Kzo9I	GATC	4 cut(s)	47, 465, 505, 1627
LmnI	GCTCC	8 cut(s)	16, 22, 139, 223, 279, 719, 946, 1171
Lsp1109I	GCAGC	2 cut(s)	83, 1211
LweI	GCATC	4 cut(s)	917, 1030, 1285, 1387
MaeI	CTAG	5 cut(s)	396, 548, 731, 1251, 1494
MaeII	ACGT	6 cut(s)	526, 598, 661, 1078, 1259, 1490
MaeIII	GTNAC	3 cut(s)	522, 922, 1496
MalI	GATC	4 cut(s)	49, 467, 507, 1629
MboI	GATC	4 cut(s)	47, 465, 505, 1627
MboII	GAAGA	8 cut(s)	76, 440, 550, 866, 896, 1649, 1775, 1829
MflI	RGATCY	1 cut(s)	465
MhlI	GDGCHC	6 cut(s)	136, 228, 252, 297, 1375, 1519
MlsI	TGGCCA	1 cut(s)	1797
MluNI	TGGCCA	1 cut(s)	1797
Mly113I	GGCGCC	1 cut(s)	328
MlyI	GAGTC	2 cut(s)	1391, 1600
MmeI	TCCRAC	2 cut(s)	208, 390
Mox20I	TGGCCA	1 cut(s)	1797
MscI	TGGCCA	1 cut(s)	1797
MslI	CAYNNNNRTG	3 cut(s)	126, 168, 1395
Msp20I	TGGCCA	1 cut(s)	1797
MspI	CCGG	4 cut(s)	20, 283, 1027, 1829
Mva1269I	GAATGC	2 cut(s)	634, 1028
MvaI	CCWGG	7 cut(s)	93, 149, 418, 576, 833, 1321, 1479
MvnI	CGCG	1 cut(s)	275
MwoI	GCNNNNNNNGC	2 cut(s)	1040, 1199
NarI	GGCGCC	1 cut(s)	328
NciI	CCSGG	3 cut(s)	284, 1028, 1829
NdeI	CATATG	1 cut(s)	1800
NdeII	GATC	4 cut(s)	47, 465, 505, 1627
NlaIV	GGNNCC	7 cut(s)	18, 281, 329, 516, 871, 1559, 1586
NmuCI	GTSAC	2 cut(s)	522, 1496
NruI	TCGCGA	1 cut(s)	275
NspI	RCATGY	4 cut(s)	167, 1121, 1526, 1715
PaqCI	CACCTGC	1 cut(s)	16
PciI	ACATGT	3 cut(s)	163, 1117, 1522
PctI	GAATGC	2 cut(s)	634, 1028
PfeI	GAWTC	5 cut(s)	155, 197, 443, 1247, 1719
PkrI	GCNGC	2 cut(s)	98, 1201
PleI	GAGTC	2 cut(s)	1390, 1600
PluTI	GGCGCC	1 cut(s)	331
PpsI	GAGTC	2 cut(s)	1390, 1600
PscI	ACATGT	3 cut(s)	163, 1117, 1522
PshBI	ATTAAT	1 cut(s)	1656
Psp124BI	GAGCTC	1 cut(s)	228
Psp6I	CCWGG	7 cut(s)	91, 147, 416, 574, 831, 1319, 1477
PspFI	CCCAGC	2 cut(s)	55, 1386
PspGI	CCWGG	7 cut(s)	91, 147, 416, 574, 831, 1319, 1477
PspN4I	GGNNCC	7 cut(s)	18, 281, 329, 516, 871, 1559, 1586
PspPI	GGNCC	2 cut(s)	1423, 1727
PstI	CTGCAG	1 cut(s)	1095
PsuI	RGATCY	1 cut(s)	465
RruI	TCGCGA	1 cut(s)	275
RsaI	GTAC	5 cut(s)	603, 775, 871, 1097, 1166
RsaNI	GTAC	5 cut(s)	602, 774, 870, 1096, 1165
RseI	CAYNNNNRTG	3 cut(s)	126, 168, 1395
SacI	GAGCTC	1 cut(s)	228
SatI	GCNGC	2 cut(s)	97, 1200
Sau3AI	GATC	4 cut(s)	47, 465, 505, 1627
Sau96I	GGNCC	2 cut(s)	1423, 1727
ScaI	AGTACT	1 cut(s)	1166
SchI	GAGTC	2 cut(s)	1391, 1600
SduI	GDGCHC	6 cut(s)	136, 228, 252, 297, 1375, 1519
SfaNI	GCATC	4 cut(s)	917, 1030, 1285, 1387
SfcI	CTRYAG	3 cut(s)	768, 1067, 1091
SfoI	GGCGCC	1 cut(s)	329
SmiMI	CAYNNNNRTG	3 cut(s)	126, 168, 1395
SmlI	CTYRAG	5 cut(s)	74, 615, 874, 1379, 1706
SmoI	CTYRAG	5 cut(s)	74, 615, 874, 1379, 1706
SsiI	CCGC	2 cut(s)	145, 1786
SspDI	GGCGCC	1 cut(s)	327
SspI	AATATT	1 cut(s)	844
SspMI	CTAG	5 cut(s)	396, 548, 731, 1251, 1494
SstI	GAGCTC	1 cut(s)	228
StyI	CCWWGG	1 cut(s)	1127
TaaI	ACNGT	5 cut(s)	650, 713, 778, 1337, 1814
TaiI	ACGT	6 cut(s)	529, 601, 664, 1081, 1262, 1493
TaqI	TCGA	2 cut(s)	5, 126
TatI	WGTACW	3 cut(s)	601, 1095, 1164
TfiI	GAWTC	5 cut(s)	155, 197, 443, 1247, 1719
TscAI	CASTG	6 cut(s)	57, 481, 525, 925, 1737, 1852
TseFI	GTSAC	2 cut(s)	522, 1496
TseI	GCWGC	2 cut(s)	96, 1199
Tsp45I	GTSAC	2 cut(s)	522, 1496
TspDTI	ATGAA	8 cut(s)	47, 158, 495, 609, 641, 954, 983, 1242
TspGWI	ACGGA	1 cut(s)	1172
TspRI	CASTG	6 cut(s)	57, 481, 525, 925, 1737, 1852
VneI	GTGCAC	2 cut(s)	293, 1515
VspI	ATTAAT	1 cut(s)	1656
XagI	CCTNNNNNAGG	1 cut(s)	274
XapI	RAATTY	5 cut(s)	609, 694, 734, 928, 1229
XbaI	TCTAGA	1 cut(s)	730
XceI	RCATGY	4 cut(s)	167, 1121, 1526, 1715
XcmI	CCANNNNNNNNNTGG	1 cut(s)	679
XmiI	GTMKAC	1 cut(s)	1650
XspI	CTAG	5 cut(s)	396, 548, 731, 1251, 1494
ZraI	GACGTC	1 cut(s)	1491
ZrmI	AGTACT	1 cut(s)	1166

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.