MD17G1285800.v1.1

Inositol hexakisphosphate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004750
Physical Location & Seq
Forward (+)
34480930 .. 34500677
19748 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1285800.v1.1.491

Sequence Viewer

Length: 3771 bp
ATGTCGATACCAAAGGAGCCGGAGCAGGTAATGAAACTAAGAGGAGGATCAGTGCTGGGCAAGAAGACCATTCTCAAGAGCGACCACTTCCCAGGCTGCCAGAACAAACGCTTGTATCCCCACATCGATGGCGCTCCCAATTACCGTCAGGCCGATTCGCTACATGTTCATGGTGTTGCCATTCCAACAATCGATGGAATCCAGAATGTTCTTAACCATATTGGAGCTCAACAAATTGATGGCAAGCGAGCACAAGTTCTTTGGATTAACCTTCGTGAGGAGCCGGTGGTCTATATTAATGGTCGCCCTTTTGTTTTGCGTGATGTGGAGAGGCCCTTTTCCAACCTTGAATATACGGGAATTAACAGGGCTAGGGTTGAACAAATGGAAGCCCGGTTAAAGGAAGATCTTTTAACTGAAGCTGCAAGATATGGAAATAAGATCCTTGTCACTGATGAACTGCCAGATGGTCAGATGGTGGATCAATGGGAACCAGTGTCACGTGATTCTGTGAAGACACCACTAGAGGTTTACGAGGAATTGCAAGAACAAGGATACCTTGTTGACTATGAACGTGTTCCTATAACTGATGAAAAGTCACCTAAGGAGCTGGATTTCGATATTTTGGTTCATAAAATTTCTCAAGCTGACATAAATGCAGAAATAATTTTTAACTGTCAAATGGGACGTGGGCGAACTACAACGGGAATGGTGATTGCAACTTTGATATACCTCAACCGTATAGGAGCTTCCGGTATTCCAAGAACCAATTCAATTGGAAAAATTTCTGAATCCAGTGAAATTGTTGGTGACAATGTTCCAAGTTCAGAAGATGCGATTCGTAGAGGTGAATATGCTGTCATAAGAAGCTTGATTCGGGTATTAGAGGGTGGCGTTGAAGGCAAGAGACAAGTGGATAAAGTCATTGACAAGTGTGCCTCTATGCAGAACTTACGTGAAGCAATTGCTACTTATCGCAATAGTATTATGCGCCAGCCAGATGAGATGAAAAAGGAAGCATCACTTTCATTTTTCATGGAGTATTTGGAACGATACTACTTCCTTATATGCTTTACCGTGTACATTCATTCAGAGGGAGCAGCCCTCCGTTCTAGTTCCTGTGATTATATTGGTTTTGCTGATTGGATGAAAGCTAGGCCTGAATTGTATAGTATTATTCGCAGGCTTCTGAGAAGAGATCCAATGGGTGCACTTGGATATGCAAGTTTGAATCCATCTTTAAAGAAGATTGCTGAATCTGCTGATGGTCGGCCTTGTGAGATGGGTGTAGTTGCTGCCTTGAGAAAGGGCGAGGTTCTTGGTAGTCAAACAGTTCTGAAAAGTGACCACTGTCCTGGTTGTCAAAACCAAAATTTACCAGAAAGAGTGGATGGTGCCCCTAATTTCCGAGAGGTCCCTGGATTTTCTGTTTATGGAGTTGCAAATCCAACAATTGATGGTATACGATCTGTCATCCACAGGATTGGTAGCTCCAAAGATGGCCGCCCAGTTTTTTGGCATAATATGAGAGAAGAACCTGTTATTTATATCAACGGAAAACCATTTGTACTTCGTGAGGTTGAAAGGCCTTACAAAAATATGCTCGAGTACACGGGTATTGATCGTGAGAGGGTGGAGAGGATGGAAGCTCGGTTAAAAGAAGATATCTTGCGAGAAGCCGATCATTATGGGGGTGCCATAATGGTTATTCATGAAACAGATGATGGGCAAATATTTGATGCTTGGGAGCATGTAAATTCTGAGGCTATTCAGACCCCGCTAGAGGTTTTTAAAGGTTTGGAGGAAGATGGTTTTCCTATCAAGTATGCGCGTGTACCCATAACGGATGGTAAAGCTCCCAAAAGTTCTGACTTTGACACATTGGCTATTAATATTGCTTCTGCTTCCAAGGACACTGCTTTTGTTTTCAATTGCCAGATGGGTAGAGGAAGGACAACCACAGGTACGGTAATTGCTTGCCTTTTGAAACTCCGAATTGACTATGGGAGACCTATCAAAATCCTGGTTGACAATATTACCCTTGAAGAGGTGGATGGTGGTAGCTCAAGTGGTGACGAAACTGGAGGTAGTAGTGCTGCATCAACCTCCAGTGTTACAAATTTTAGAAATGAAAAGGAGCAAAGCCGTGTATTCGGCATGAATGACATCCTCTTGTTGTGGAAAATAACAAGATTATTTGATAATGGGGTGGAATGCCGGGAGGCCTTAGATGCTATTATAGATAGATGTTCTGCTCTACAGAACATACGTCAGGCTGTTCTGCAGTATAGAAAGGTATTCAATCAACAACATGTTGAGCCAAGGGTAAGGAGGGTGGCACTAAATCGTGGTGCTGAGTACTTGGAGCGCTACTTCCGTTTAATTGCTTTTGCAGCATACTTAGGAAGTGAAGCATTTGATGGATTCTGTGGGCAAGGAGAATCTAGGATGACGTTTAAGAATTGGTTGCATCAGAGACCAGAGGTTCAAGCAATGAAATGGAGCATAAGACTAAGACCTGGACGCTTTTTTACTGTCCCTGAGGAGTTGAGAGCACCACATGAGTCCCAACATGGAGATGCTGTCATGGAGGCCATTATCAAGGCCCGTAACGGTTCTGTTTTGGGGAAAGGTTCTATACTTAAAATGTATTTCTTTCCAGGTCAAAGGACGTCTAGTCACATTCAAATCCATGGTGCACCACATGTTTATAAGGTGGATGGATATCCTGTGTATAGCATGGCTACACCAACAATTCTTGGTGCCAAAGAGATGTTGGCATATTTAGGTGCCAAACCCAAAGCAGAAGGATCTGCTGCTCAGAAAGTGGTTTTAACTGATCTGAGAGAAGAAGCAATTGTCTACATTAATGGTACACCATTTGTCCTACGGGAGCTAAACAAACCTGTTGATACACTCAAGCATGTGGGAATCACAGGCTCAGTGGTGGAACACATGGAAGCACGACTAAAAGAAGATATACTATCTGAGGTCAGGCAGTCCGGTGGCCGTATGCTTTTACACCGTGAAGAATATAGCCCGGCATTAAACCAGTCCAGTGTCATAGGATACTTAGAAAACATCTTTGCAGATGATGTTAAGACACCTGCTGAAGTATATGCCGCTCTGAAAGATGAGGGTTATAATATTGCATACAGGAGAATACCATTAACTAGAGAAAGAGAGGCTTTAGCGTCTGATGTGGATGCAATTCAATACTGCATAGATGACTCTGCAGGGTGTTACCTTTTTGTATCACACACAGGGTTTGGAGGAGTTGCATATGCAATGGCTATTATTTGTATCAGAATTGGTGCAGAAACAAATTCCCTACCAAAGGATCCACAACCTTTGGTTGGAACAAACCTCATGTGTACACCTGAAGAGGATTTGCCTTCTCGAGCTTCTGATGAAGAAGTTCTGAGGATGGGTGACTACCGAGACATACTAAGCCTTACAAGAGTTCTTGTGTACGGTCCTAAGAGCAAAGCAGACGTTGACATTGTTATTGAAAGGTGTGCAGGTGCGGGACATCTGCGAGATGACATTCTTTATTATAGTAAGGAACTCAAAAAGTTCCCTGATGCTGATGACGAGCAACGAGCATGTCTCATGGACATGGGGATCAAAGCATTAAAGCGGTACTTTTTCCTAATCACATTTAGGTCCTACCTTTACTGCACTTGTGCGGCTGATATAAAGTTCACATCATGGATGGACGCGAGGCCTGAACTAGGACATCTATGTAATAACCTTAGGATTGATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTPlike_phytase

Protein Analysis

1257

Amino Acids

141.17

Weight (kDa)

6.09

Isoelectric Point (pI)

38.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTPlike_phytase PF14566 89 - 243 6.3e-57 Inositol hexakisphosphate
PTPlike_phytase PF14566 507 - 663 2.4e-57 Inositol hexakisphosphate
Tc-R-P PF22785 580 - 662 5.7e-06 Polymorphic toxin system, DSP-PTPase phosphatase
PTPlike_phytase PF14566 947 - 1102 2.2e-35 Inositol hexakisphosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2712, 3144
AarI CACCTGC 2 cut(s) 3115, 3512
AatII GACGTC 1 cut(s) 2675
Acc36I ACCTGC 3 cut(s) 16, 3115, 3512
AccB1I GGYRCC 4 cut(s) 1394, 1694, 2762, 2789
AccBSI CCGCTC 1 cut(s) 3125
AccI GTMKAC 2 cut(s) 1462, 2862
AccII CGCG 2 cut(s) 1831, 3722
AciI CCGC 6 cut(s) 1504, 1778, 3123, 3527, 3640, 3689
AclWI GGATC 8 cut(s) 55, 436, 489, 1193, 2818, 3335, 3348, 3632
AcoI YGGCCR 2 cut(s) 1501, 3007
AcsI RAATTY 6 cut(s) 636, 783, 1372, 1756, 2119, 3325
AcuI CTGAAG 3 cut(s) 438, 3132, 3402
AcvI CACGTG 1 cut(s) 503
AcyI GRCGYC 1 cut(s) 2672
AfeI AGCGCT 1 cut(s) 2369
AfiI CCNNNNNNNGG 7 cut(s) 277, 400, 1783, 2047, 3337, 3356, 3734
AflIII ACRYGT 4 cut(s) 163, 574, 2311, 2704
AhdI GACNNNNNGTC 1 cut(s) 2676
AjiI CACGTC 1 cut(s) 689
AjnI CCWGG 6 cut(s) 91, 1354, 1417, 2022, 2518, 2659
AjuI GAANNNNNNNTTGG 2 cut(s) 754, 786
Alw21I GWGCWC 5 cut(s) 229, 253, 1213, 2557, 2701
Alw26I GTCTC 5 cut(s) 901, 2002, 2470, 3435, 3614
Alw44I GTGCAC 2 cut(s) 1209, 2697
AlwI GGATC 8 cut(s) 55, 436, 489, 1193, 2818, 3335, 3348, 3632
Ama87I CYCGRG 2 cut(s) 1604, 3399
Aor51HI AGCGCT 1 cut(s) 2369
ApaLI GTGCAC 2 cut(s) 1209, 2697
ApeKI GCWGC 7 cut(s) 96, 422, 1100, 1295, 2096, 2393, 2816
ApoI RAATTY 6 cut(s) 636, 783, 1372, 1756, 2119, 3325
ArsI GACNNNNNNTTYG 2 cut(s) 1904, 1936
AseI ATTAAT 3 cut(s) 297, 1890, 2868
Asp700I GAANNNNTTC 4 cut(s) 576, 769, 784, 3417
AspLEI GCGC 4 cut(s) 134, 993, 1831, 2370
AspS9I GGNCC 5 cut(s) 333, 1414, 2605, 3476, 3666
AsuC2I CCSGG 3 cut(s) 394, 2219, 3041
AsuHPI GGTGA 6 cut(s) 591, 724, 821, 860, 2084, 3443
AvaI CYCGRG 2 cut(s) 1604, 3399
AvaII GGWCC 3 cut(s) 1414, 3476, 3666
AxyI CCTNAGG 3 cut(s) 603, 2541, 3755
BaeGI GKGCMC 3 cut(s) 1213, 1399, 2701
BaeI ACNNNNGTAYC 2 cut(s) 98, 131
BamHI GGATCC 1 cut(s) 3340
BanI GGYRCC 4 cut(s) 1394, 1694, 2762, 2789
BanII GRGCYC 1 cut(s) 229
BarI GAAGNNNNNNTAC 2 cut(s) 733, 765
BbrPI CACGTG 1 cut(s) 503
BbsI GAAGAC 2 cut(s) 71, 521
Bbv12I GWGCWC 5 cut(s) 229, 253, 1213, 2557, 2701
BbvI GCAGC 7 cut(s) 83, 409, 1112, 1282, 2083, 2405, 2803
BceAI ACGGC 2 cut(s) 2130, 2994
BciT130I CCWGG 6 cut(s) 93, 1356, 1419, 2024, 2520, 2661
BciVI GTATCC 3 cut(s) 126, 548, 3062
BcnI CCSGG 3 cut(s) 394, 2219, 3041
BcoDI GTCTC 5 cut(s) 901, 2002, 2470, 3435, 3614
BfaI CTAG 9 cut(s) 372, 524, 1113, 1155, 1781, 2445, 2676, 3174, 3734
BfmI CTRYAG 3 cut(s) 2258, 2282, 3234
BfoI RGCGCY 2 cut(s) 135, 2371
BfuAI ACCTGC 3 cut(s) 16, 3115, 3512
BfuI GTATCC 3 cut(s) 126, 548, 3062
BglII AGATCT 1 cut(s) 406
BmcAI AGTACT 1 cut(s) 2360
Bme1390I CCNGG 9 cut(s) 93, 394, 1356, 1419, 2024, 2219, 2520, 2661, 3041
Bme18I GGWCC 3 cut(s) 1414, 3476, 3666
BmeRI GACNNNNNGTC 1 cut(s) 2676
BmeT110I CYCGRG 2 cut(s) 1604, 3399
BmgBI CACGTC 1 cut(s) 689
BmgT120I GGNCC 5 cut(s) 333, 1414, 2605, 3476, 3666
BmiI GGNNCC 9 cut(s) 18, 282, 492, 1396, 1416, 1696, 2764, 2791, 3342
BmrFI CCNGG 9 cut(s) 93, 394, 1356, 1419, 2024, 2219, 2520, 2661, 3041
BmrI ACTGGG 1 cut(s) 1502
BmsI GCATC 9 cut(s) 823, 1028, 1729, 2108, 2221, 2479, 2569, 3196, 3574
BmuI ACTGGG 1 cut(s) 1502
BoxI GACNNNNGTC 1 cut(s) 1350
BpiI GAAGAC 2 cut(s) 71, 521
BplI GAGNNNNNCTC 4 cut(s) 1089, 1121, 3594, 3626
BpmI CTGGAG 2 cut(s) 2092, 2103
BpuEI CTTGAG 5 cut(s) 59, 627, 1321, 2050, 2903
BpuMI CCSGG 3 cut(s) 394, 2219, 3041
Bsa29I ATCGAT 2 cut(s) 126, 192
BsaAI YACGTR 2 cut(s) 503, 956
BsaBI GATNNNNATC 1 cut(s) 2724
BsaHI GRCGYC 1 cut(s) 2672
BsaI GGTCTC 2 cut(s) 2002, 2470
BsaJI CCNNGG 5 cut(s) 91, 1417, 1908, 2321, 2692
BsaWI WCCGGW 2 cut(s) 752, 3002
BsaXI ACNNNNNCTCC 4 cut(s) 36, 66, 2568, 2598
Bsc4I CCNNNNNNNGG 7 cut(s) 277, 400, 1783, 2047, 3337, 3356, 3734
Bse118I RCCGGY 1 cut(s) 283
Bse1I ACTGG 7 cut(s) 494, 795, 1508, 2086, 2109, 3052, 3057
Bse21I CCTNAGG 3 cut(s) 603, 2541, 3755
Bse3DI GCAATG 2 cut(s) 2499, 3294
Bse8I GATNNNNATC 1 cut(s) 2724
BseBI CCWGG 6 cut(s) 93, 1356, 1419, 2024, 2520, 2661
BseCI ATCGAT 2 cut(s) 126, 192
BseDI CCNNGG 5 cut(s) 91, 1417, 1908, 2321, 2692
BseJI GATNNNNATC 1 cut(s) 2724
BseLI CCNNNNNNNGG 7 cut(s) 277, 400, 1783, 2047, 3337, 3356, 3734
BseMI GCAATG 2 cut(s) 2499, 3294
BseMII CTCAG 9 cut(s) 1181, 1752, 2346, 2532, 2834, 2834, 2955, 2979, 3413
BseNI ACTGG 7 cut(s) 494, 795, 1508, 2086, 2109, 3052, 3057
BseRI GAGGAG 4 cut(s) 57, 293, 2558, 3288
BseSI GKGCMC 3 cut(s) 1213, 1399, 2701
BseXI GCAGC 7 cut(s) 83, 409, 1112, 1282, 2083, 2405, 2803
BseYI CCCAGC 1 cut(s) 55
BsgI GTGCAG 3 cut(s) 3336, 3540, 3664
Bsh1236I CGCG 2 cut(s) 1831, 3722
BshNI GGYRCC 4 cut(s) 1394, 1694, 2762, 2789
BshVI ATCGAT 2 cut(s) 126, 192
BsiHKAI GWGCWC 5 cut(s) 229, 253, 1213, 2557, 2701
BsiHKCI CYCGRG 2 cut(s) 1604, 3399
BsiSI CCGG 7 cut(s) 20, 284, 394, 753, 2218, 3003, 3041
BslFI GGGAC 5 cut(s) 699, 1400, 2522, 2551, 3543
BslI CCNNNNNNNGG 7 cut(s) 277, 400, 1783, 2047, 3337, 3356, 3734
BsmAI GTCTC 5 cut(s) 901, 2002, 2470, 3435, 3614
BsmFI GGGAC 5 cut(s) 699, 1400, 2522, 2551, 3543
BsmI GAATGC 1 cut(s) 2219
Bso31I GGTCTC 2 cut(s) 2002, 2470
BsoBI CYCGRG 2 cut(s) 1604, 3399
Bsp1286I GDGCHC 6 cut(s) 229, 253, 1213, 1399, 2557, 2701
Bsp1407I TGTACA 2 cut(s) 1080, 3374
Bsp19I CCATGG 1 cut(s) 2692
BspACI CCGC 6 cut(s) 1504, 1778, 3123, 3527, 3640, 3689
BspCNI CTCAG 9 cut(s) 1182, 1753, 2347, 2533, 2833, 2835, 2954, 2980, 3414
BspDI ATCGAT 2 cut(s) 126, 192
BspFNI CGCG 2 cut(s) 1831, 3722
BspHI TCATGA 1 cut(s) 1711
BspLI GGNNCC 9 cut(s) 18, 282, 492, 1396, 1416, 1696, 2764, 2791, 3342
BspMAI CTGCAG 2 cut(s) 2286, 3238
BspMI ACCTGC 3 cut(s) 16, 3115, 3512
BspPI GGATC 8 cut(s) 55, 436, 489, 1193, 2818, 3335, 3348, 3632
BspT107I GGYRCC 4 cut(s) 1394, 1694, 2762, 2789
BspTNI GGTCTC 2 cut(s) 2002, 2470
BsrBI CCGCTC 1 cut(s) 3125
BsrDI GCAATG 2 cut(s) 2499, 3294
BsrFI RCCGGY 1 cut(s) 283
BsrGI TGTACA 2 cut(s) 1080, 3374
BsrI ACTGG 7 cut(s) 494, 795, 1508, 2086, 2109, 3052, 3057
BssAI RCCGGY 1 cut(s) 283
BssECI CCNNGG 5 cut(s) 91, 1417, 1908, 2321, 2692
BssNAI GTATAC 1 cut(s) 1463
BssNI GRCGYC 1 cut(s) 2672
BssT1I CCWWGG 3 cut(s) 1908, 2321, 2692
Bst1107I GTATAC 1 cut(s) 1463
Bst2UI CCWGG 6 cut(s) 93, 1356, 1419, 2024, 2520, 2661
Bst6I CTCTTC 3 cut(s) 1189, 2040, 3378
BstACI GRCGYC 1 cut(s) 2672
BstAUI TGTACA 2 cut(s) 1080, 3374
BstBAI YACGTR 2 cut(s) 503, 956
BstC8I GCNNGC 5 cut(s) 245, 249, 995, 1184, 1978
BstDSI CCRYGG 1 cut(s) 2692
BstENI CCTNNNNNAGG 4 cut(s) 275, 2045, 3335, 3732
BstFNI CGCG 2 cut(s) 1831, 3722
BstH2I RGCGCY 2 cut(s) 135, 2371
BstHHI GCGC 4 cut(s) 134, 993, 1831, 2370
BstMAI GTCTC 5 cut(s) 901, 2002, 2470, 3435, 3614
BstMWI GCNNNNNNNGC 4 cut(s) 900, 1259, 2231, 2393
BstNI CCWGG 6 cut(s) 93, 1356, 1419, 2024, 2520, 2661
BstNSI RCATGY 6 cut(s) 167, 1754, 2315, 2708, 2927, 3609
BstPAI GACNNNNGTC 1 cut(s) 1350
BstSCI CCNGG 9 cut(s) 91, 392, 1354, 1417, 2022, 2217, 2518, 2659, 3039
BstSFI CTRYAG 3 cut(s) 2258, 2282, 3234
BstSLI GKGCMC 3 cut(s) 1213, 1399, 2701
BstUI CGCG 2 cut(s) 1831, 3722
BstV1I GCAGC 7 cut(s) 83, 409, 1112, 1282, 2083, 2405, 2803
BstV2I GAAGAC 2 cut(s) 71, 521
BstX2I RGATCY 5 cut(s) 406, 441, 1198, 2810, 3340
BstXI CCANNNNNNTGG 4 cut(s) 128, 1355, 1484, 1515
BstYI RGATCY 5 cut(s) 406, 441, 1198, 2810, 3340
BstZ17I GTATAC 1 cut(s) 1463
Bsu15I ATCGAT 2 cut(s) 126, 192
Bsu36I CCTNAGG 3 cut(s) 603, 2541, 3755
BsuI GTATCC 3 cut(s) 126, 548, 3062
BsuTUI ATCGAT 2 cut(s) 126, 192
BtgI CCRYGG 1 cut(s) 2692
BtrI CACGTC 1 cut(s) 689
BtsI GCAGTG 1 cut(s) 1914
BtsIMutI CAGTG 9 cut(s) 57, 450, 501, 802, 1348, 1914, 2116, 2949, 3064
BveI ACCTGC 3 cut(s) 16, 3115, 3512
Cac8I GCNNGC 5 cut(s) 245, 249, 995, 1184, 1978
CciI TCATGA 1 cut(s) 1711
CfoI GCGC 4 cut(s) 134, 993, 1831, 2370
Cfr10I RCCGGY 1 cut(s) 283
Cfr13I GGNCC 5 cut(s) 333, 1414, 2605, 3476, 3666
ClaI ATCGAT 2 cut(s) 126, 192
CseI GACGC 3 cut(s) 2532, 3183, 3728
CspCI CAANNNNNGTGG 2 cut(s) 3333, 3368
DraI TTTAAA 2 cut(s) 1242, 1792
DriI GACNNNNNGTC 1 cut(s) 2676
EaeI YGGCCR 2 cut(s) 1501, 3007
Eam1104I CTCTTC 3 cut(s) 1189, 2040, 3378
Eam1105I GACNNNNNGTC 1 cut(s) 2676
EarI CTCTTC 3 cut(s) 1189, 2040, 3378
Ecl136II GAGCTC 1 cut(s) 227
Eco130I CCWWGG 3 cut(s) 1908, 2321, 2692
Eco147I AGGCCT 4 cut(s) 1159, 1588, 2225, 3727
Eco24I GRGCYC 1 cut(s) 229
Eco31I GGTCTC 2 cut(s) 2002, 2470
Eco32I GATATC 2 cut(s) 1666, 2726
Eco47I GGWCC 3 cut(s) 1414, 3476, 3666
Eco47III AGCGCT 1 cut(s) 2369
Eco53kI GAGCTC 1 cut(s) 227
Eco57I CTGAAG 3 cut(s) 438, 3132, 3402
Eco72I CACGTG 1 cut(s) 503
Eco81I CCTNAGG 3 cut(s) 603, 2541, 3755
Eco88I CYCGRG 2 cut(s) 1604, 3399
EcoICRI GAGCTC 1 cut(s) 227
EcoNI CCTNNNNNAGG 4 cut(s) 275, 2045, 3335, 3732
EcoO109I RGGNCCY 3 cut(s) 333, 1414, 3666
EcoRII CCWGG 6 cut(s) 91, 1354, 1417, 2022, 2518, 2659
EcoRV GATATC 2 cut(s) 1666, 2726
EcoT14I CCWWGG 3 cut(s) 1908, 2321, 2692
EcoT38I GRGCYC 1 cut(s) 229
ErhI CCWWGG 3 cut(s) 1908, 2321, 2692
FalI AAGNNNNNCTT 8 cut(s) 543, 575, 1008, 1040, 3172, 3204, 3629, 3661
FaqI GGGAC 5 cut(s) 699, 1400, 2522, 2551, 3543
FauI CCCGC 2 cut(s) 1785, 3520
FauNDI CATATG 1 cut(s) 3283
FblI GTMKAC 2 cut(s) 1462, 2862
FriOI GRGCYC 1 cut(s) 229
FspBI CTAG 9 cut(s) 372, 524, 1113, 1155, 1781, 2445, 2676, 3174, 3734
GlaI GCGC 4 cut(s) 133, 992, 1830, 2369
GsaI CCCAGC 1 cut(s) 59
GsuI CTGGAG 2 cut(s) 2092, 2103
HaeII RGCGCY 2 cut(s) 135, 2371
HapII CCGG 7 cut(s) 20, 284, 394, 753, 2218, 3003, 3041
HgaI GACGC 3 cut(s) 2532, 3183, 3728
HhaI GCGC 4 cut(s) 134, 993, 1831, 2370
Hin1I GRCGYC 1 cut(s) 2672
Hin6I GCGC 4 cut(s) 132, 991, 1829, 2368
HinP1I GCGC 4 cut(s) 132, 991, 1829, 2368
HincII GTYRAC 3 cut(s) 565, 2029, 3499
HindII GTYRAC 3 cut(s) 565, 2029, 3499
HindIII AAGCTT 1 cut(s) 868
HpaII CCGG 7 cut(s) 20, 284, 394, 753, 2218, 3003, 3041
HphI GGTGA 6 cut(s) 591, 724, 821, 860, 2084, 3443
Hpy188III TCNNGA 7 cut(s) 76, 202, 275, 1574, 1625, 1712, 3399
HpyAV CCTTC 5 cut(s) 281, 893, 1944, 2801, 3405
HpyCH4IV ACGT 8 cut(s) 502, 574, 688, 955, 2269, 2453, 2672, 3495
HpyF10VI GCNNNNNNNGC 4 cut(s) 900, 1259, 2231, 2393
HpySE526I ACGT 8 cut(s) 502, 574, 688, 955, 2269, 2453, 2672, 3495
Hsp92I GRCGYC 1 cut(s) 2672
HspAI GCGC 4 cut(s) 132, 991, 1829, 2368
Lsp1109I GCAGC 7 cut(s) 83, 409, 1112, 1282, 2083, 2405, 2803
LweI GCATC 9 cut(s) 823, 1028, 1729, 2108, 2221, 2479, 2569, 3196, 3574
MaeI CTAG 9 cut(s) 372, 524, 1113, 1155, 1781, 2445, 2676, 3174, 3734
MaeII ACGT 8 cut(s) 502, 574, 688, 955, 2269, 2453, 2672, 3495
MbiI CCGCTC 1 cut(s) 3125
MfeI CAATTG 5 cut(s) 774, 963, 1452, 1930, 2856
MflI RGATCY 5 cut(s) 406, 441, 1198, 2810, 3340
MhlI GDGCHC 6 cut(s) 229, 253, 1213, 1399, 2557, 2701
MlyI GAGTC 2 cut(s) 2573, 3224
MmeI TCCRAC 4 cut(s) 209, 366, 1472, 3337
MroXI GAANNNNTTC 4 cut(s) 576, 769, 784, 3417
MslI CAYNNNNRTG 4 cut(s) 126, 168, 2577, 3617
MspI CCGG 7 cut(s) 20, 284, 394, 753, 2218, 3003, 3041
MspR9I CCNGG 9 cut(s) 93, 394, 1356, 1419, 2024, 2219, 2520, 2661, 3041
MunI CAATTG 5 cut(s) 774, 963, 1452, 1930, 2856
Mva1269I GAATGC 1 cut(s) 2219
MvaI CCWGG 6 cut(s) 93, 1356, 1419, 2024, 2520, 2661
MvnI CGCG 2 cut(s) 1831, 3722
MwoI GCNNNNNNNGC 4 cut(s) 900, 1259, 2231, 2393
NciI CCSGG 3 cut(s) 394, 2219, 3041
NcoI CCATGG 1 cut(s) 2692
NdeI CATATG 1 cut(s) 3283
NlaIV GGNNCC 9 cut(s) 18, 282, 492, 1396, 1416, 1696, 2764, 2791, 3342
NmuCI GTSAC 8 cut(s) 448, 498, 597, 809, 1343, 2072, 2678, 3431
NspI RCATGY 6 cut(s) 167, 1754, 2315, 2708, 2927, 3609
PaeR7I CTCGAG 2 cut(s) 1604, 3399
PagI TCATGA 1 cut(s) 1711
PaqCI CACCTGC 2 cut(s) 3115, 3512
PceI AGGCCT 4 cut(s) 1159, 1588, 2225, 3727
PciI ACATGT 3 cut(s) 163, 2311, 2704
PctI GAATGC 1 cut(s) 2219
PdmI GAANNNNTTC 4 cut(s) 576, 769, 784, 3417
PleI GAGTC 2 cut(s) 2572, 3224
PmaCI CACGTG 1 cut(s) 503
PmlI CACGTG 1 cut(s) 503
PpsI GAGTC 2 cut(s) 2572, 3224
Ppu21I YACGTR 2 cut(s) 503, 956
PpuMI RGGWCCY 2 cut(s) 1414, 3666
PscI ACATGT 3 cut(s) 163, 2311, 2704
PshAI GACNNNNGTC 1 cut(s) 1350
PshBI ATTAAT 3 cut(s) 297, 1890, 2868
PsiI TTATAA 2 cut(s) 2712, 3144
Psp124BI GAGCTC 1 cut(s) 229
Psp5II RGGWCCY 2 cut(s) 1414, 3666
Psp6I CCWGG 6 cut(s) 91, 1354, 1417, 2022, 2518, 2659
PspCI CACGTG 1 cut(s) 503
PspFI CCCAGC 1 cut(s) 55
PspGI CCWGG 6 cut(s) 91, 1354, 1417, 2022, 2518, 2659
PspN4I GGNNCC 9 cut(s) 18, 282, 492, 1396, 1416, 1696, 2764, 2791, 3342
PspPI GGNCC 5 cut(s) 333, 1414, 2605, 3476, 3666
PspPPI RGGWCCY 2 cut(s) 1414, 3666
PspXI VCTCGAGB 1 cut(s) 1604
PstI CTGCAG 2 cut(s) 2286, 3238
PsuI RGATCY 5 cut(s) 406, 441, 1198, 2810, 3340
RseI CAYNNNNRTG 4 cut(s) 126, 168, 2577, 3617
SacI GAGCTC 1 cut(s) 229
Sau96I GGNCC 5 cut(s) 333, 1414, 2605, 3476, 3666
ScaI AGTACT 1 cut(s) 2360
SchI GAGTC 2 cut(s) 2573, 3224
ScrFI CCNGG 9 cut(s) 93, 394, 1356, 1419, 2024, 2219, 2520, 2661, 3041
SduI GDGCHC 6 cut(s) 229, 253, 1213, 1399, 2557, 2701
SfaNI GCATC 9 cut(s) 823, 1028, 1729, 2108, 2221, 2479, 2569, 3196, 3574
SfcI CTRYAG 3 cut(s) 2258, 2282, 3234
Sfr274I CTCGAG 2 cut(s) 1604, 3399
SinI GGWCC 3 cut(s) 1414, 3476, 3666
SlaI CTCGAG 2 cut(s) 1604, 3399
SmiMI CAYNNNNRTG 4 cut(s) 126, 168, 2577, 3617
SmlI CTYRAG 7 cut(s) 74, 642, 1300, 1604, 2065, 2918, 3399
SmoI CTYRAG 7 cut(s) 74, 642, 1300, 1604, 2065, 2918, 3399
SseBI AGGCCT 4 cut(s) 1159, 1588, 2225, 3727
SsiI CCGC 6 cut(s) 1504, 1778, 3123, 3527, 3640, 3689
SspI AATATT 4 cut(s) 1734, 1894, 2035, 3148
SspMI CTAG 9 cut(s) 372, 524, 1113, 1155, 1781, 2445, 2676, 3174, 3734
SstI GAGCTC 1 cut(s) 229
StuI AGGCCT 4 cut(s) 1159, 1588, 2225, 3727
StyD4I CCNGG 9 cut(s) 91, 392, 1354, 1417, 2022, 2217, 2518, 2659, 3039
StyI CCWWGG 3 cut(s) 1908, 2321, 2692
TaiI ACGT 8 cut(s) 505, 577, 691, 958, 2272, 2456, 2675, 3498
TaqI TCGA 6 cut(s) 5, 126, 192, 618, 1605, 3400
TatI WGTACW 5 cut(s) 1080, 1567, 1608, 2358, 3374
TauI GCSGC 3 cut(s) 1506, 3125, 3692
TscAI CASTG 9 cut(s) 57, 457, 501, 802, 1355, 1921, 2116, 2949, 3064
TseFI GTSAC 8 cut(s) 448, 498, 597, 809, 1343, 2072, 2678, 3431
TseI GCWGC 7 cut(s) 96, 422, 1100, 1295, 2096, 2393, 2816
Tsp45I GTSAC 8 cut(s) 448, 498, 597, 809, 1343, 2072, 2678, 3431
TspGWI ACGGA 4 cut(s) 1097, 1569, 1859, 2366
TspRI CASTG 9 cut(s) 57, 457, 501, 802, 1355, 1921, 2116, 2949, 3064
VneI GTGCAC 2 cut(s) 1209, 2697
VpaK11BI GGWCC 3 cut(s) 1414, 3476, 3666
VspI ATTAAT 3 cut(s) 297, 1890, 2868
XagI CCTNNNNNAGG 4 cut(s) 275, 2045, 3335, 3732
XapI RAATTY 6 cut(s) 636, 783, 1372, 1756, 2119, 3325
XceI RCATGY 6 cut(s) 167, 1754, 2315, 2708, 2927, 3609
XcmI CCANNNNNNNNNTGG 1 cut(s) 2773
XhoI CTCGAG 2 cut(s) 1604, 3399
XmiI GTMKAC 2 cut(s) 1462, 2862
XmnI GAANNNNTTC 4 cut(s) 576, 769, 784, 3417
XspI CTAG 9 cut(s) 372, 524, 1113, 1155, 1781, 2445, 2676, 3174, 3734
ZraI GACGTC 1 cut(s) 2673
ZrmI AGTACT 1 cut(s) 2360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.