MD10G1003300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0014240
Physical Location & Seq
Reverse (-)
646526 .. 648280
1755 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1003300.v1.1.491

Sequence Viewer

Length: 327 bp
ATGGCAACCGAGGAAAAGGGAGAAGAATTGCAACAAACCACAAGTCCCCAAGATGCTGCCGGTTTGAGAGGAGCTGTGGAAGCAAAGTACCGGCAAATCAAAGAGCATGCAGAGACCTACCCTTATGTTTGGGCTTCCTACATTGTTGTATACGGTGGTTTTGCTCTCTGGGGTACCTACAGATGGAGAAGGCTTCGCAAGACTGAGGATCGTGTACGTGCCCTTCAGGAGAGATTACGCAAACATTATGCAGCAGAAGAGGCTTGTGCTAGCTCTTCCACCCCAGCTGTTAAGGTTCCACCATCTGCTGATAAAATCTCCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

109

Amino Acids

12.28

Weight (kDa)

9.07

Isoelectric Point (pI)

68.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014240)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09270 AT5G09270
fragaria_vesca FvH4_6g45500
malus_domestica MD09G1082400.v1.1 MD10G1003300.v1.1
prunus_persica Prupe.3G241300_v2.0.a1
pyrus_communis pycom09g00890 pycom10g00270
rosa_chinensis RchiOBHm_Chr2g0163441
rosa_laevigata RLG00000021393
rosa_multiflora Rmu_sc0028601.1_g000002 Rmu_ssc0000231.1_g000002
rosa_roxburghii Rroxscaffold_2G00087330
rosa_rugosa Rorug02G0502700
rosa_samantha Rh2AG569700 Rh2CG551600
rosa_wichuraiana Rw2G047150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 173
AccB1I GGYRCC 1 cut(s) 173
AccI GTMKAC 1 cut(s) 150
AclWI GGATC 1 cut(s) 216
AcuI CTGAAG 1 cut(s) 209
AfaI GTAC 3 cut(s) 89, 175, 216
AfiI CCNNNNNNNGG 1 cut(s) 183
AluBI AGCT 3 cut(s) 74, 273, 287
AluI AGCT 3 cut(s) 74, 273, 287
Alw26I GTCTC 1 cut(s) 107
AlwI GGATC 1 cut(s) 216
ApeKI GCWGC 2 cut(s) 56, 251
ArsI GACNNNNNNTTYG 2 cut(s) 28, 60
Asp718I GGTACC 1 cut(s) 173
AsuNHI GCTAGC 1 cut(s) 269
BaeGI GKGCMC 1 cut(s) 223
BanI GGYRCC 1 cut(s) 173
BarI GAAGNNNNNNTAC 2 cut(s) 207, 239
BbvI GCAGC 2 cut(s) 43, 263
BccI CCATC 2 cut(s) 177, 310
BcoDI GTCTC 1 cut(s) 107
BfaI CTAG 2 cut(s) 270, 325
BfmI CTRYAG 1 cut(s) 178
BisI GCNGC 2 cut(s) 57, 252
BlsI GCNGC 2 cut(s) 58, 253
BmiI GGNNCC 2 cut(s) 175, 297
BmsI GCATC 1 cut(s) 43
BmtI GCTAGC 1 cut(s) 273
BsaAI YACGTR 1 cut(s) 218
BsaI GGTCTC 1 cut(s) 107
BsaJI CCNNGG 1 cut(s) 9
Bsc4I CCNNNNNNNGG 1 cut(s) 183
Bse118I RCCGGY 2 cut(s) 59, 90
BseDI CCNNGG 1 cut(s) 9
BseLI CCNNNNNNNGG 1 cut(s) 183
BseMII CTCAG 1 cut(s) 195
BseRI GAGGAG 1 cut(s) 84
BseSI GKGCMC 1 cut(s) 223
BseXI GCAGC 2 cut(s) 43, 263
BseYI CCCAGC 1 cut(s) 283
BshNI GGYRCC 1 cut(s) 173
BsiSI CCGG 2 cut(s) 60, 91
BslFI GGGAC 1 cut(s) 30
BslI CCNNNNNNNGG 1 cut(s) 183
BsmAI GTCTC 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 30
Bso31I GGTCTC 1 cut(s) 107
Bsp1286I GDGCHC 1 cut(s) 223
Bsp143I GATC 1 cut(s) 208
BspCNI CTCAG 1 cut(s) 196
BspLI GGNNCC 2 cut(s) 175, 297
BspOI GCTAGC 1 cut(s) 273
BspPI GGATC 1 cut(s) 216
BspQI GCTCTTC 1 cut(s) 280
BspT107I GGYRCC 1 cut(s) 173
BspTNI GGTCTC 1 cut(s) 107
BsrFI RCCGGY 2 cut(s) 59, 90
BssAI RCCGGY 2 cut(s) 59, 90
BssECI CCNNGG 1 cut(s) 9
BssMI GATC 1 cut(s) 208
BssNAI GTATAC 1 cut(s) 151
Bst1107I GTATAC 1 cut(s) 151
Bst4CI ACNGT 1 cut(s) 155
Bst6I CTCTTC 2 cut(s) 252, 280
BstBAI YACGTR 1 cut(s) 218
BstC8I GCNNGC 2 cut(s) 108, 271
BstDEI CTNAG 1 cut(s) 204
BstKTI GATC 1 cut(s) 211
BstMAI GTCTC 1 cut(s) 107
BstMBI GATC 1 cut(s) 208
BstMWI GCNNNNNNNGC 2 cut(s) 80, 260
BstNSI RCATGY 1 cut(s) 110
BstSFI CTRYAG 1 cut(s) 178
BstSLI GKGCMC 1 cut(s) 223
BstV1I GCAGC 2 cut(s) 43, 263
BstZ17I GTATAC 1 cut(s) 151
Cac8I GCNNGC 2 cut(s) 108, 271
Cfr10I RCCGGY 2 cut(s) 59, 90
Csp6I GTAC 3 cut(s) 88, 174, 215
CviAII CATG 1 cut(s) 107
CviJI RGCY 6 cut(s) 74, 134, 193, 263, 273, 287
CviKI_1 RGCY 6 cut(s) 74, 134, 193, 263, 273, 287
CviQI GTAC 3 cut(s) 88, 174, 215
DdeI CTNAG 1 cut(s) 204
DpnI GATC 1 cut(s) 210
DpnII GATC 1 cut(s) 208
Eam1104I CTCTTC 2 cut(s) 252, 280
EarI CTCTTC 2 cut(s) 252, 280
Eco31I GGTCTC 1 cut(s) 107
Eco57I CTGAAG 1 cut(s) 209
FaeI CATG 1 cut(s) 110
FaiI YATR 4 cut(s) 108, 126, 151, 249
FaqI GGGAC 1 cut(s) 30
FatI CATG 1 cut(s) 106
FblI GTMKAC 1 cut(s) 150
Fnu4HI GCNGC 2 cut(s) 57, 252
Fsp4HI GCNGC 2 cut(s) 57, 252
FspBI CTAG 2 cut(s) 270, 325
GluI GCNGC 2 cut(s) 57, 252
GsaI CCCAGC 1 cut(s) 287
HapII CCGG 2 cut(s) 60, 91
Hin1II CATG 1 cut(s) 110
HpaII CCGG 2 cut(s) 60, 91
Hpy166II GTNNAC 2 cut(s) 151, 215
Hpy188III TCNNGA 1 cut(s) 227
Hpy8I GTNNAC 2 cut(s) 151, 215
HpyAV CCTTC 2 cut(s) 183, 233
HpyCH4III ACNGT 1 cut(s) 155
HpyCH4IV ACGT 1 cut(s) 217
HpyCH4V TGCA 3 cut(s) 31, 110, 251
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 260
HpyF3I CTNAG 1 cut(s) 204
HpySE526I ACGT 1 cut(s) 217
Hsp92II CATG 1 cut(s) 110
KpnI GGTACC 1 cut(s) 177
Kzo9I GATC 1 cut(s) 208
LguI GCTCTTC 1 cut(s) 280
LmnI GCTCC 1 cut(s) 71
LpnPI CCDG 5 cut(s) 73, 104, 154, 212, 297
Lsp1109I GCAGC 2 cut(s) 43, 263
LweI GCATC 1 cut(s) 43
MaeI CTAG 2 cut(s) 270, 325
MaeII ACGT 1 cut(s) 217
MalI GATC 1 cut(s) 210
MboI GATC 1 cut(s) 208
MboII GAAGA 3 cut(s) 35, 267, 269
MhlI GDGCHC 1 cut(s) 223
MluCI AATT 1 cut(s) 26
MnlI CCTC 4 cut(s) 4, 62, 199, 253
MseI TTAA 1 cut(s) 291
MspA1I CMGCKG 1 cut(s) 287
MspI CCGG 2 cut(s) 60, 91
MwoI GCNNNNNNNGC 2 cut(s) 80, 260
NdeII GATC 1 cut(s) 208
NheI GCTAGC 1 cut(s) 269
NlaIII CATG 1 cut(s) 110
NlaIV GGNNCC 2 cut(s) 175, 297
NspI RCATGY 1 cut(s) 110
PaeI GCATGC 1 cut(s) 110
PciSI GCTCTTC 1 cut(s) 280
PkrI GCNGC 2 cut(s) 58, 253
Ppu21I YACGTR 1 cut(s) 218
PspFI CCCAGC 1 cut(s) 283
PspN4I GGNNCC 2 cut(s) 175, 297
PvuII CAGCTG 1 cut(s) 287
RsaI GTAC 3 cut(s) 89, 175, 216
RsaNI GTAC 3 cut(s) 88, 174, 215
SapI GCTCTTC 1 cut(s) 280
SaqAI TTAA 1 cut(s) 291
SatI GCNGC 2 cut(s) 57, 252
Sau3AI GATC 1 cut(s) 208
SduI GDGCHC 1 cut(s) 223
SetI ASST 7 cut(s) 76, 119, 179, 220, 275, 289, 297
SfaNI GCATC 1 cut(s) 43
SfcI CTRYAG 1 cut(s) 178
SphI GCATGC 1 cut(s) 110
Sse9I AATT 1 cut(s) 26
SspMI CTAG 2 cut(s) 270, 325
TaaI ACNGT 1 cut(s) 155
TaiI ACGT 1 cut(s) 220
TasI AATT 1 cut(s) 26
Tru1I TTAA 1 cut(s) 291
Tru9I TTAA 1 cut(s) 291
TseI GCWGC 2 cut(s) 56, 251
XceI RCATGY 1 cut(s) 110
XmiI GTMKAC 1 cut(s) 150
XspI CTAG 2 cut(s) 270, 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.