RLG00000021393

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014240
Physical Location & Seq
Forward (+)
74812992 .. 74813345
354 bp
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UTR
Exon/CDS
Intron
RLM00000021393

Sequence Viewer

Length: 354 bp
ATGGCAACTCAGGAAACTGGAGAAGAAAATCAGCAAAGTGCGAGTTCCCAAGCTGTTGGTTTCATCGGAGCGGTGGAAGCAAAGTATAGGCAAATCAAAGAGCATGCAGAGACCTACCCTTATGTGTGGGCCTCCTACAGTGTTGTATATGGTGGTTTGGCCCTCTGGACTACCTACAGATGGAGAAAGCTTCGAAAGACTGAGGATCGAGTACGAGCCCTTCAGGAGAGATTACGCAAGCATTATGCAGCTGAAGAGCATGCAAGTGCAACCGTAAGCTCAAGCACAAATCGAAGCCCTGCCACGCCAGTTCAAAAGGTCCCTCCACCATCTGCTGACAAAACCCCTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

118

Amino Acids

13.17

Weight (kDa)

9.56

Isoelectric Point (pI)

57.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014240)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09270 AT5G09270
fragaria_vesca FvH4_6g45500
malus_domestica MD09G1082400.v1.1 MD10G1003300.v1.1
prunus_persica Prupe.3G241300_v2.0.a1
pyrus_communis pycom09g00890 pycom10g00270
rosa_chinensis RchiOBHm_Chr2g0163441
rosa_laevigata RLG00000021393
rosa_multiflora Rmu_sc0028601.1_g000002 Rmu_ssc0000231.1_g000002
rosa_roxburghii Rroxscaffold_2G00087330
rosa_rugosa Rorug02G0502700
rosa_samantha Rh2AG569700 Rh2CG551600
rosa_wichuraiana Rw2G047150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 71
AciI CCGC 1 cut(s) 71
AclWI GGATC 1 cut(s) 213
AcuI CTGAAG 2 cut(s) 206, 273
AfaI GTAC 1 cut(s) 213
AfiI CCNNNNNNNGG 1 cut(s) 180
AgsI TTSAA 1 cut(s) 314
AluBI AGCT 4 cut(s) 53, 190, 251, 279
AluI AGCT 4 cut(s) 53, 190, 251, 279
Alw26I GTCTC 1 cut(s) 104
AlwI GGATC 1 cut(s) 213
AoxI GGCC 2 cut(s) 129, 159
ApeKI GCWGC 1 cut(s) 248
AspS9I GGNCC 3 cut(s) 129, 160, 319
AsuII TTCGAA 1 cut(s) 193
AvaII GGWCC 1 cut(s) 319
BanII GRGCYC 1 cut(s) 220
BarI GAAGNNNNNNTAC 2 cut(s) 204, 236
BbvI GCAGC 1 cut(s) 260
BccI CCATC 2 cut(s) 174, 337
BcoDI GTCTC 1 cut(s) 104
BfmI CTRYAG 2 cut(s) 136, 175
BisI GCNGC 1 cut(s) 249
BlsI GCNGC 1 cut(s) 250
Bme18I GGWCC 1 cut(s) 319
BmgT120I GGNCC 3 cut(s) 129, 160, 319
BmiI GGNNCC 1 cut(s) 321
BpmI CTGGAG 1 cut(s) 39
Bpu14I TTCGAA 1 cut(s) 193
BpuEI CTTGAG 1 cut(s) 265
BsaI GGTCTC 1 cut(s) 104
Bsc4I CCNNNNNNNGG 1 cut(s) 180
Bse1I ACTGG 2 cut(s) 22, 308
BseLI CCNNNNNNNGG 1 cut(s) 180
BseMII CTCAG 2 cut(s) 23, 192
BseNI ACTGG 2 cut(s) 22, 308
BseXI GCAGC 1 cut(s) 260
BshFI GGCC 2 cut(s) 131, 161
BslFI GGGAC 1 cut(s) 305
BslI CCNNNNNNNGG 1 cut(s) 180
BsmAI GTCTC 1 cut(s) 104
BsmFI GGGAC 1 cut(s) 305
BsnI GGCC 2 cut(s) 131, 161
Bso31I GGTCTC 1 cut(s) 104
Bsp119I TTCGAA 1 cut(s) 193
Bsp1286I GDGCHC 1 cut(s) 220
Bsp143I GATC 1 cut(s) 205
BspACI CCGC 1 cut(s) 71
BspANI GGCC 2 cut(s) 131, 161
BspCNI CTCAG 2 cut(s) 22, 193
BspLI GGNNCC 1 cut(s) 321
BspPI GGATC 1 cut(s) 213
BspQI GCTCTTC 1 cut(s) 249
BspT104I TTCGAA 1 cut(s) 193
BspTNI GGTCTC 1 cut(s) 104
BsrBI CCGCTC 1 cut(s) 71
BsrI ACTGG 2 cut(s) 22, 308
BssMI GATC 1 cut(s) 205
Bst4CI ACNGT 2 cut(s) 140, 274
Bst6I CTCTTC 1 cut(s) 249
BstBI TTCGAA 1 cut(s) 193
BstC8I GCNNGC 3 cut(s) 105, 239, 261
BstDEI CTNAG 2 cut(s) 9, 201
BstKTI GATC 1 cut(s) 208
BstMAI GTCTC 1 cut(s) 104
BstMBI GATC 1 cut(s) 205
BstMWI GCNNNNNNNGC 1 cut(s) 77
BstNSI RCATGY 2 cut(s) 107, 263
BstSFI CTRYAG 2 cut(s) 136, 175
BstV1I GCAGC 1 cut(s) 260
BstXI CCANNNNNNTGG 1 cut(s) 56
BsuRI GGCC 2 cut(s) 131, 161
BtsIMutI CAGTG 1 cut(s) 145
Cac8I GCNNGC 3 cut(s) 105, 239, 261
Cfr13I GGNCC 3 cut(s) 129, 160, 319
Csp6I GTAC 1 cut(s) 212
CviAII CATG 2 cut(s) 104, 260
CviJI RGCY 8 cut(s) 53, 131, 161, 190, 218, 251, 279, 297
CviKI_1 RGCY 8 cut(s) 53, 131, 161, 190, 218, 251, 279, 297
CviQI GTAC 1 cut(s) 212
DdeI CTNAG 2 cut(s) 9, 201
DpnI GATC 1 cut(s) 207
DpnII GATC 1 cut(s) 205
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
Eco24I GRGCYC 1 cut(s) 220
Eco31I GGTCTC 1 cut(s) 104
Eco47I GGWCC 1 cut(s) 319
Eco57I CTGAAG 2 cut(s) 206, 273
EcoO109I RGGNCCY 1 cut(s) 319
EcoT38I GRGCYC 1 cut(s) 220
FaeI CATG 2 cut(s) 107, 263
FaiI YATR 7 cut(s) 87, 105, 123, 148, 150, 246, 261
FaqI GGGAC 1 cut(s) 305
FatI CATG 2 cut(s) 103, 259
Fnu4HI GCNGC 1 cut(s) 249
FriOI GRGCYC 1 cut(s) 220
Fsp4HI GCNGC 1 cut(s) 249
GluI GCNGC 1 cut(s) 249
GsuI CTGGAG 1 cut(s) 39
HaeIII GGCC 2 cut(s) 131, 161
Hin1II CATG 2 cut(s) 107, 263
HindIII AAGCTT 1 cut(s) 188
Hpy188I TCNGA 1 cut(s) 68
Hpy188III TCNNGA 3 cut(s) 11, 166, 224
HpyAV CCTTC 1 cut(s) 230
HpyCH4III ACNGT 2 cut(s) 140, 274
HpyCH4V TGCA 4 cut(s) 107, 248, 263, 269
HpyF10VI GCNNNNNNNGC 1 cut(s) 77
HpyF3I CTNAG 2 cut(s) 9, 201
Hsp92II CATG 2 cut(s) 107, 263
Kzo9I GATC 1 cut(s) 205
LguI GCTCTTC 1 cut(s) 249
LmnI GCTCC 1 cut(s) 68
LpnPI CCDG 5 cut(s) 3, 151, 209, 312, 321
Lsp1109I GCAGC 1 cut(s) 260
MalI GATC 1 cut(s) 207
MbiI CCGCTC 1 cut(s) 71
MboI GATC 1 cut(s) 205
MboII GAAGA 2 cut(s) 35, 266
MhlI GDGCHC 1 cut(s) 220
MnlI CCTC 4 cut(s) 142, 173, 196, 333
MslI CAYNNNNRTG 1 cut(s) 264
MspA1I CMGCKG 1 cut(s) 251
MwoI GCNNNNNNNGC 1 cut(s) 77
NdeII GATC 1 cut(s) 205
NlaIII CATG 2 cut(s) 107, 263
NlaIV GGNNCC 1 cut(s) 321
NspI RCATGY 2 cut(s) 107, 263
NspV TTCGAA 1 cut(s) 193
PaeI GCATGC 2 cut(s) 107, 263
PciSI GCTCTTC 1 cut(s) 249
PkrI GCNGC 1 cut(s) 250
PpuMI RGGWCCY 1 cut(s) 319
Psp5II RGGWCCY 1 cut(s) 319
PspN4I GGNNCC 1 cut(s) 321
PspPI GGNCC 3 cut(s) 129, 160, 319
PspPPI RGGWCCY 1 cut(s) 319
PvuII CAGCTG 1 cut(s) 251
RsaI GTAC 1 cut(s) 213
RsaNI GTAC 1 cut(s) 212
RseI CAYNNNNRTG 1 cut(s) 264
SapI GCTCTTC 1 cut(s) 249
SatI GCNGC 1 cut(s) 249
Sau3AI GATC 1 cut(s) 205
Sau96I GGNCC 3 cut(s) 129, 160, 319
SduI GDGCHC 1 cut(s) 220
SetI ASST 7 cut(s) 55, 116, 176, 192, 253, 281, 321
SfcI CTRYAG 2 cut(s) 136, 175
SfuI TTCGAA 1 cut(s) 193
SinI GGWCC 1 cut(s) 319
SmiMI CAYNNNNRTG 1 cut(s) 264
SmlI CTYRAG 1 cut(s) 280
SmoI CTYRAG 1 cut(s) 280
SphI GCATGC 2 cut(s) 107, 263
SsiI CCGC 1 cut(s) 71
TaaI ACNGT 2 cut(s) 140, 274
TaqI TCGA 3 cut(s) 193, 208, 292
TscAI CASTG 1 cut(s) 145
TseI GCWGC 1 cut(s) 248
TspDTI ATGAA 1 cut(s) 52
TspRI CASTG 1 cut(s) 145
VpaK11BI GGWCC 1 cut(s) 319
XceI RCATGY 2 cut(s) 107, 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.