MD10G1029900.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0019740
Physical Location & Seq
Forward (+)
3927324 .. 3927758
435 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1029900.v1.1.491

Sequence Viewer

Length: 339 bp
ATGTTTACAAGTGTTCCATCTGGGGATGCCATTTTGTTGAAGTGGGTACTTCACAATTGGAGTGACCAACACTGCCTAAAGCTGTTGGAAAATTGTTACAACGCTATTCCAGACGATGGAAAAGTGATTATTGTGGAAGCACTTGTTCCAGTGATGCCAGAGACTAGCACCGCCGTGAAGAGCACCTCCCTACTTGATGTGCTTATGCTGAGTCAAAAAAGGGGAGGAAAGGAGCGGAGCCGAGAAGAGTTCATGACTTTGGCAACTGGTGCTGGATTTAGTGGCATTAAATATGAATGTTTTGTCGCCAGCCTTTGGGTGATGGAGATCTTTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01058 ko01100 ko01110 map00940 map01058 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

113

Amino Acids

12.48

Weight (kDa)

5.62

Isoelectric Point (pI)

34.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 1 - 94 2.1e-29 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019740)

Species Orthologous Gene IDs
malus_domestica MD10G1029900.v1.1
pyrus_communis pycom01g11380 pycom10g21340
rosa_multiflora Rmu_sc0010379.1_g000002
rosa_roxburghii Rroxscaffold_4G00295260
rosa_samantha Rh1CG320300 Rh1DG336700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 116, 315
AccBSI CCGCTC 1 cut(s) 235
AciI CCGC 2 cut(s) 171, 235
AfaI GTAC 1 cut(s) 48
AfiI CCNNNNNNNGG 2 cut(s) 116, 315
AgsI TTSAA 1 cut(s) 40
AleI CACNNNNGTG 1 cut(s) 173
AluBI AGCT 1 cut(s) 82
AluI AGCT 1 cut(s) 82
Alw21I GWGCWC 1 cut(s) 185
Alw26I GTCTC 1 cut(s) 155
AsuHPI GGTGA 1 cut(s) 331
Bbv12I GWGCWC 1 cut(s) 185
BccI CCATC 3 cut(s) 25, 110, 316
BceAI ACGGC 1 cut(s) 158
BcoDI GTCTC 1 cut(s) 155
BfaI CTAG 1 cut(s) 165
BglII AGATCT 1 cut(s) 327
BmiI GGNNCC 1 cut(s) 239
BmsI GCATC 2 cut(s) 16, 144
BsaBI GATNNNNATC 1 cut(s) 326
Bsc4I CCNNNNNNNGG 2 cut(s) 116, 315
Bse1I ACTGG 2 cut(s) 149, 271
Bse8I GATNNNNATC 1 cut(s) 326
BseGI GGATG 1 cut(s) 31
BseJI GATNNNNATC 1 cut(s) 326
BseLI CCNNNNNNNGG 2 cut(s) 116, 315
BseMII CTCAG 1 cut(s) 200
BseNI ACTGG 2 cut(s) 149, 271
BsiHKAI GWGCWC 1 cut(s) 185
BslI CCNNNNNNNGG 2 cut(s) 116, 315
BsmAI GTCTC 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 185
Bsp143I GATC 1 cut(s) 327
BspACI CCGC 2 cut(s) 171, 235
BspCNI CTCAG 1 cut(s) 201
BspHI TCATGA 1 cut(s) 252
BspLI GGNNCC 1 cut(s) 239
BspQI GCTCTTC 1 cut(s) 173
BsrBI CCGCTC 1 cut(s) 235
BsrI ACTGG 2 cut(s) 149, 271
BssMI GATC 1 cut(s) 327
Bst6I CTCTTC 2 cut(s) 173, 240
BstAPI GCANNNNNTGC 1 cut(s) 269
BstC8I GCNNGC 1 cut(s) 310
BstDEI CTNAG 1 cut(s) 209
BstF5I GGATG 1 cut(s) 31
BstKTI GATC 1 cut(s) 330
BstMAI GTCTC 1 cut(s) 155
BstMBI GATC 1 cut(s) 327
BstMWI GCNNNNNNNGC 1 cut(s) 269
BstX2I RGATCY 1 cut(s) 327
BstYI RGATCY 1 cut(s) 327
BtsCI GGATG 1 cut(s) 31
BtsI GCAGTG 1 cut(s) 70
BtsIMutI CAGTG 2 cut(s) 70, 156
Cac8I GCNNGC 1 cut(s) 310
CciI TCATGA 1 cut(s) 252
Csp6I GTAC 1 cut(s) 47
CviAII CATG 1 cut(s) 253
CviJI RGCY 3 cut(s) 82, 240, 312
CviKI_1 RGCY 3 cut(s) 82, 240, 312
CviQI GTAC 1 cut(s) 47
DdeI CTNAG 1 cut(s) 209
DpnI GATC 1 cut(s) 329
DpnII GATC 1 cut(s) 327
Eam1104I CTCTTC 2 cut(s) 173, 240
EarI CTCTTC 2 cut(s) 173, 240
FaeI CATG 1 cut(s) 256
FaiI YATR 3 cut(s) 206, 254, 294
FatI CATG 1 cut(s) 252
FokI GGATG 1 cut(s) 38
FspBI CTAG 1 cut(s) 165
Hin1II CATG 1 cut(s) 256
HinfI GANTC 1 cut(s) 211
HphI GGTGA 1 cut(s) 331
Hpy166II GTNNAC 1 cut(s) 6
Hpy188III TCNNGA 2 cut(s) 110, 253
Hpy8I GTNNAC 1 cut(s) 6
HpyF10VI GCNNNNNNNGC 1 cut(s) 269
HpyF3I CTNAG 1 cut(s) 209
Hsp92II CATG 1 cut(s) 256
Kzo9I GATC 1 cut(s) 327
LguI GCTCTTC 1 cut(s) 173
LmnI GCTCC 2 cut(s) 232, 237
LpnPI CCDG 7 cut(s) 6, 123, 162, 171, 252, 258, 322
LweI GCATC 2 cut(s) 16, 144
MaeI CTAG 1 cut(s) 165
MaeIII GTNAC 2 cut(s) 62, 95
MalI GATC 1 cut(s) 329
MbiI CCGCTC 1 cut(s) 235
MboI GATC 1 cut(s) 327
MboII GAAGA 2 cut(s) 190, 257
MfeI CAATTG 1 cut(s) 55
MflI RGATCY 1 cut(s) 327
MhlI GDGCHC 1 cut(s) 185
MluCI AATT 2 cut(s) 55, 91
MlyI GAGTC 1 cut(s) 220
MmeI TCCRAC 1 cut(s) 66
MnlI CCTC 2 cut(s) 196, 218
MseI TTAA 2 cut(s) 288, 333
MslI CAYNNNNRTG 1 cut(s) 173
MunI CAATTG 1 cut(s) 55
MwoI GCNNNNNNNGC 1 cut(s) 269
NdeII GATC 1 cut(s) 327
NlaIII CATG 1 cut(s) 256
NlaIV GGNNCC 1 cut(s) 239
NmeAIII GCCGAG 1 cut(s) 266
NmuCI GTSAC 1 cut(s) 62
OliI CACNNNNGTG 1 cut(s) 173
PagI TCATGA 1 cut(s) 252
PciSI GCTCTTC 1 cut(s) 173
PflMI CCANNNNNTGG 2 cut(s) 116, 315
PleI GAGTC 1 cut(s) 219
PpsI GAGTC 1 cut(s) 219
PspN4I GGNNCC 1 cut(s) 239
PsuI RGATCY 1 cut(s) 327
RsaI GTAC 1 cut(s) 48
RsaNI GTAC 1 cut(s) 47
RseI CAYNNNNRTG 1 cut(s) 173
SapI GCTCTTC 1 cut(s) 173
SaqAI TTAA 2 cut(s) 288, 333
Sau3AI GATC 1 cut(s) 327
SchI GAGTC 1 cut(s) 220
SduI GDGCHC 1 cut(s) 185
SetI ASST 2 cut(s) 84, 188
SfaNI GCATC 2 cut(s) 16, 144
SmiMI CAYNNNNRTG 1 cut(s) 173
Sse9I AATT 2 cut(s) 55, 91
SsiI CCGC 2 cut(s) 171, 235
SspMI CTAG 1 cut(s) 165
TasI AATT 2 cut(s) 55, 91
Tru1I TTAA 2 cut(s) 288, 333
Tru9I TTAA 2 cut(s) 288, 333
TscAI CASTG 2 cut(s) 77, 156
TseFI GTSAC 1 cut(s) 62
Tsp45I GTSAC 1 cut(s) 62
TspDTI ATGAA 2 cut(s) 241, 309
TspRI CASTG 2 cut(s) 77, 156
Van91I CCANNNNNTGG 2 cut(s) 116, 315
XspI CTAG 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.