pycom10g21340
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0019740
Physical Location & Seq
Reverse (-)
24004781 .. 24005329
549 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g21340.1

Sequence Viewer

Length: 351 bp
ATGTTTGAGTCAATACCACATGCCCAGTCGATAATGATGAAGTGGGTACTTCATAACTGGGCTGATGACGTGTGCATTAAGTTGCTGAAAAACTGTTGGAATGCGTTACCGGAAACTGGGAAGGTAATAGTTGTGGAATTTGTAGTACCTGAAGTACTGGAAAATACACCAGAAACAAGGAACAAACTCGCACTAGACATGACGATGATGGCCCTTTTTCCTGGGGGCAAGGAGCGAACAGCGGTTGAATTTGATAACCTAGCAAAATCTGCAGGTTTTGTTGGAACAAAGCTTTTTCCAATTTCATATGGATATCATGCGATTGAGTTTATCAAGGGTAAACCATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

117

Amino Acids

13.1

Weight (kDa)

6.28

Isoelectric Point (pI)

25.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 1 - 93 2.7e-26 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019740)

Species Orthologous Gene IDs
malus_domestica MD10G1029900.v1.1
pyrus_communis pycom01g11380 pycom10g21340
rosa_multiflora Rmu_sc0010379.1_g000002
rosa_roxburghii Rroxscaffold_4G00295260
rosa_samantha Rh1CG320300 Rh1DG336700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 263
AciI CCGC 1 cut(s) 242
AcsI RAATTY 2 cut(s) 137, 248
AcuI CTGAAG 1 cut(s) 171
AfaI GTAC 3 cut(s) 48, 147, 156
AfiI CCNNNNNNNGG 1 cut(s) 116
AflIII ACRYGT 1 cut(s) 69
AgsI TTSAA 1 cut(s) 248
AjiI CACGTC 1 cut(s) 70
AjnI CCWGG 1 cut(s) 220
AluBI AGCT 1 cut(s) 292
AluI AGCT 1 cut(s) 292
AoxI GGCC 1 cut(s) 210
ApoI RAATTY 2 cut(s) 137, 248
AspS9I GGNCC 1 cut(s) 211
BccI CCATC 1 cut(s) 202
BciT130I CCWGG 1 cut(s) 222
BfaI CTAG 2 cut(s) 194, 260
BfmI CTRYAG 1 cut(s) 270
BfuAI ACCTGC 1 cut(s) 263
BmcAI AGTACT 1 cut(s) 156
Bme1390I CCNGG 1 cut(s) 222
BmgBI CACGTC 1 cut(s) 70
BmgT120I GGNCC 1 cut(s) 211
BmrFI CCNGG 1 cut(s) 222
BmrI ACTGGG 3 cut(s) 19, 67, 126
BmuI ACTGGG 3 cut(s) 19, 67, 126
BsaJI CCNNGG 1 cut(s) 221
BsaWI WCCGGW 1 cut(s) 109
Bsc4I CCNNNNNNNGG 1 cut(s) 116
Bse1I ACTGG 4 cut(s) 25, 62, 121, 162
BseBI CCWGG 1 cut(s) 222
BseDI CCNNGG 1 cut(s) 221
BseLI CCNNNNNNNGG 1 cut(s) 116
BseNI ACTGG 4 cut(s) 25, 62, 121, 162
BshFI GGCC 1 cut(s) 212
BsiSI CCGG 1 cut(s) 110
BslI CCNNNNNNNGG 1 cut(s) 116
BsmI GAATGC 1 cut(s) 106
BsnI GGCC 1 cut(s) 212
BspACI CCGC 1 cut(s) 242
BspANI GGCC 1 cut(s) 212
BspMAI CTGCAG 1 cut(s) 274
BspMI ACCTGC 1 cut(s) 263
BsrI ACTGG 4 cut(s) 25, 62, 121, 162
BssECI CCNNGG 1 cut(s) 221
Bst2UI CCWGG 1 cut(s) 222
Bst4CI ACNGT 1 cut(s) 95
BstAPI GCANNNNNTGC 1 cut(s) 269
BstMWI GCNNNNNNNGC 1 cut(s) 269
BstNI CCWGG 1 cut(s) 222
BstNSI RCATGY 1 cut(s) 23
BstSCI CCNGG 1 cut(s) 220
BstSFI CTRYAG 1 cut(s) 270
BsuRI GGCC 1 cut(s) 212
BtrI CACGTC 1 cut(s) 70
BveI ACCTGC 1 cut(s) 263
Cfr13I GGNCC 1 cut(s) 211
Csp6I GTAC 3 cut(s) 47, 146, 155
CviAII CATG 3 cut(s) 20, 199, 317
CviJI RGCY 3 cut(s) 62, 212, 292
CviKI_1 RGCY 3 cut(s) 62, 212, 292
CviQI GTAC 3 cut(s) 47, 146, 155
Eco32I GATATC 1 cut(s) 314
Eco57I CTGAAG 1 cut(s) 171
EcoRII CCWGG 1 cut(s) 220
EcoRV GATATC 1 cut(s) 314
FaeI CATG 3 cut(s) 23, 202, 320
FaiI YATR 6 cut(s) 21, 54, 200, 307, 309, 318
FatI CATG 3 cut(s) 19, 198, 316
FauNDI CATATG 1 cut(s) 307
FspBI CTAG 2 cut(s) 194, 260
HaeIII GGCC 1 cut(s) 212
HapII CCGG 1 cut(s) 110
Hin1II CATG 3 cut(s) 23, 202, 320
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 1 cut(s) 8
HpaII CCGG 1 cut(s) 110
Hpy166II GTNNAC 1 cut(s) 341
Hpy8I GTNNAC 1 cut(s) 341
HpyAV CCTTC 1 cut(s) 115
HpyCH4III ACNGT 1 cut(s) 95
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 2 cut(s) 75, 272
HpyF10VI GCNNNNNNNGC 1 cut(s) 269
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 3 cut(s) 23, 202, 320
LmnI GCTCC 1 cut(s) 232
MaeI CTAG 2 cut(s) 194, 260
MaeII ACGT 1 cut(s) 69
MaeIII GTNAC 1 cut(s) 105
MluCI AATT 3 cut(s) 137, 248, 300
MlyI GAGTC 1 cut(s) 17
MmeI TCCRAC 2 cut(s) 77, 262
MseI TTAA 1 cut(s) 78
MslI CAYNNNNRTG 1 cut(s) 203
MspA1I CMGCKG 1 cut(s) 242
MspI CCGG 1 cut(s) 110
MspR9I CCNGG 1 cut(s) 222
Mva1269I GAATGC 1 cut(s) 106
MvaI CCWGG 1 cut(s) 222
MwoI GCNNNNNNNGC 1 cut(s) 269
NdeI CATATG 1 cut(s) 307
NlaIII CATG 3 cut(s) 23, 202, 320
NspI RCATGY 1 cut(s) 23
PctI GAATGC 1 cut(s) 106
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
Psp6I CCWGG 1 cut(s) 220
PspGI CCWGG 1 cut(s) 220
PspPI GGNCC 1 cut(s) 211
PstI CTGCAG 1 cut(s) 274
RsaI GTAC 3 cut(s) 48, 147, 156
RsaNI GTAC 3 cut(s) 47, 146, 155
RseI CAYNNNNRTG 1 cut(s) 203
SaqAI TTAA 1 cut(s) 78
Sau96I GGNCC 1 cut(s) 211
ScaI AGTACT 1 cut(s) 156
SchI GAGTC 1 cut(s) 17
ScrFI CCNGG 1 cut(s) 222
SetI ASST 6 cut(s) 72, 126, 151, 261, 277, 294
SfcI CTRYAG 1 cut(s) 270
SmiMI CAYNNNNRTG 1 cut(s) 203
Sse9I AATT 3 cut(s) 137, 248, 300
SsiI CCGC 1 cut(s) 242
SspMI CTAG 2 cut(s) 194, 260
StyD4I CCNGG 1 cut(s) 220
TaaI ACNGT 1 cut(s) 95
TaiI ACGT 1 cut(s) 72
TaqI TCGA 1 cut(s) 29
TasI AATT 3 cut(s) 137, 248, 300
TatI WGTACW 1 cut(s) 154
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TspDTI ATGAA 3 cut(s) 41, 53, 294
XapI RAATTY 2 cut(s) 137, 248
XceI RCATGY 1 cut(s) 23
XspI CTAG 2 cut(s) 194, 260
ZrmI AGTACT 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.