MD10G1084900.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0015886
Physical Location & Seq
Reverse (-)
12481070 .. 12483114
2045 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1084900.v1.1.491

Sequence Viewer

Length: 477 bp
ATGCAAAAAGGGCTGGCCATGGATGAGGTACCTGGATCAGTAGGGACGAGTGCCAGCTTCTCATTGAGATTGGGTCAGGCCATCTTTTCCTCTGCTTCTCTTCTCTTCATGTCTTTGGGTGTGGAGTTCTACAGCTACACTGCTTTCTGCTATTTGGTAACAGTCATGGGTCTGGTCATACCTTGGAGTTTCACTTTGGCATTGGTGGATGGTTACTCTGTGCTCGTGAAGTGCCCTCTTCGTCAGCCTGGAATACTGCTGATCATCGTTGTTGGAGATTGGGTATTATCCATTCTCACTCTAGCTGCAGCTTGCTCAACGACTAGCGTTGTGGATATCATGCTCCATGCTAACGGTCCATACTGCCCTCCAAAGTTTTGCAGCCGATATAAAATATCGGCTGCGATGGCCTTCATGTCATGGTTTCTGTCTTTGGCTTCGTCTTTTTTCAATCTTTGGTTACTTCCGTCCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

159

Amino Acids

17.13

Weight (kDa)

7.6

Isoelectric Point (pI)

31.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 14 - 143 1.9e-27 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015886)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49405
fragaria_vesca FvH4_2g07710
malus_domestica MD10G1084900.v1.1
prunus_persica Prupe.8G107800_v2.0.a1 Prupe.8G107800_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0262031
rosa_laevigata RLG00000014459
rosa_multiflora Rmu_sc0006019.1_g000002
rosa_roxburghii Rroxscaffold_7G00207760
rosa_rugosa Rorug05G0595100
rosa_samantha Rh6AG114500 Rh6BG108700 Rh6CG106000 Rh6DG096400
rosa_wichuraiana Rw6G009820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 28
AccB1I GGYRCC 1 cut(s) 28
AclWI GGATC 1 cut(s) 43
AcoI YGGCCR 1 cut(s) 15
AfaI GTAC 1 cut(s) 30
AgsI TTSAA 1 cut(s) 451
AjnI CCWGG 2 cut(s) 31, 247
AluBI AGCT 4 cut(s) 57, 135, 305, 311
AluI AGCT 4 cut(s) 57, 135, 305, 311
Alw21I GWGCWC 1 cut(s) 225
AlwI GGATC 1 cut(s) 43
AoxI GGCC 3 cut(s) 15, 78, 408
ApeKI GCWGC 4 cut(s) 305, 308, 381, 401
Asp718I GGTACC 1 cut(s) 28
AspS9I GGNCC 1 cut(s) 356
AvaII GGWCC 1 cut(s) 356
BaeGI GKGCMC 1 cut(s) 236
BalI TGGCCA 1 cut(s) 17
BanI GGYRCC 1 cut(s) 28
BauI CACGAG 1 cut(s) 224
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 4 cut(s) 292, 320, 388, 393
BccI CCATC 3 cut(s) 89, 203, 400
BciT130I CCWGG 2 cut(s) 33, 249
BclI TGATCA 1 cut(s) 261
BfaI CTAG 2 cut(s) 302, 324
BfmI CTRYAG 2 cut(s) 130, 306
BisI GCNGC 4 cut(s) 306, 309, 382, 402
BlsI GCNGC 4 cut(s) 307, 310, 383, 403
Bme1390I CCNGG 2 cut(s) 33, 249
Bme18I GGWCC 1 cut(s) 356
BmgT120I GGNCC 1 cut(s) 356
BmiI GGNNCC 1 cut(s) 30
BmrFI CCNGG 2 cut(s) 33, 249
BsaJI CCNNGG 2 cut(s) 18, 182
BsaXI ACNNNNNCTCC 2 cut(s) 267, 297
BseBI CCWGG 2 cut(s) 33, 249
BseDI CCNNGG 2 cut(s) 18, 182
BseGI GGATG 2 cut(s) 28, 214
BseSI GKGCMC 1 cut(s) 236
BseXI GCAGC 4 cut(s) 292, 320, 388, 393
BshFI GGCC 3 cut(s) 17, 80, 410
BshNI GGYRCC 1 cut(s) 28
BsiHKAI GWGCWC 1 cut(s) 225
BslFI GGGAC 1 cut(s) 58
BsmFI GGGAC 1 cut(s) 58
BsnI GGCC 3 cut(s) 17, 80, 410
Bsp1286I GDGCHC 2 cut(s) 225, 236
Bsp143I GATC 2 cut(s) 35, 261
Bsp19I CCATGG 1 cut(s) 18
BspANI GGCC 3 cut(s) 17, 80, 410
BspLI GGNNCC 1 cut(s) 30
BspMAI CTGCAG 1 cut(s) 310
BspPI GGATC 1 cut(s) 43
BspT107I GGYRCC 1 cut(s) 28
BssECI CCNNGG 2 cut(s) 18, 182
BssMI GATC 2 cut(s) 35, 261
BssSI CACGAG 1 cut(s) 224
BssT1I CCWWGG 2 cut(s) 18, 182
Bst2BI CACGAG 1 cut(s) 224
Bst2UI CCWGG 2 cut(s) 33, 249
Bst4CI ACNGT 2 cut(s) 163, 356
Bst6I CTCTTC 3 cut(s) 105, 110, 243
BstC8I GCNNGC 3 cut(s) 15, 55, 313
BstDSI CCRYGG 1 cut(s) 18
BstF5I GGATG 2 cut(s) 28, 214
BstKTI GATC 2 cut(s) 38, 264
BstMBI GATC 2 cut(s) 35, 261
BstMWI GCNNNNNNNGC 2 cut(s) 10, 407
BstNI CCWGG 2 cut(s) 33, 249
BstSCI CCNGG 2 cut(s) 31, 247
BstSFI CTRYAG 2 cut(s) 130, 306
BstSLI GKGCMC 1 cut(s) 236
BstV1I GCAGC 4 cut(s) 292, 320, 388, 393
BsuRI GGCC 3 cut(s) 17, 80, 410
BtgI CCRYGG 1 cut(s) 18
BtgZI GCGATG 1 cut(s) 419
BtsCI GGATG 2 cut(s) 28, 214
BtsI GCAGTG 1 cut(s) 138
BtsIMutI CAGTG 1 cut(s) 138
Cac8I GCNNGC 3 cut(s) 15, 55, 313
Cfr13I GGNCC 1 cut(s) 356
Csp6I GTAC 1 cut(s) 29
CviAII CATG 7 cut(s) 19, 109, 166, 340, 347, 415, 420
CviQI GTAC 1 cut(s) 29
DpnI GATC 2 cut(s) 37, 263
DpnII GATC 2 cut(s) 35, 261
EaeI YGGCCR 1 cut(s) 15
Eam1104I CTCTTC 3 cut(s) 105, 110, 243
EarI CTCTTC 3 cut(s) 105, 110, 243
Eco130I CCWWGG 2 cut(s) 18, 182
Eco32I GATATC 1 cut(s) 337
Eco47I GGWCC 1 cut(s) 356
EcoRII CCWGG 2 cut(s) 31, 247
EcoRV GATATC 1 cut(s) 337
EcoT14I CCWWGG 2 cut(s) 18, 182
ErhI CCWWGG 2 cut(s) 18, 182
FaeI CATG 7 cut(s) 22, 112, 169, 343, 350, 418, 423
FaqI GGGAC 1 cut(s) 58
FatI CATG 7 cut(s) 18, 108, 165, 339, 346, 414, 419
FbaI TGATCA 1 cut(s) 261
Fnu4HI GCNGC 4 cut(s) 306, 309, 382, 402
FokI GGATG 2 cut(s) 35, 221
Fsp4HI GCNGC 4 cut(s) 306, 309, 382, 402
FspBI CTAG 2 cut(s) 302, 324
GluI GCNGC 4 cut(s) 306, 309, 382, 402
HaeIII GGCC 3 cut(s) 17, 80, 410
Hin1II CATG 7 cut(s) 22, 112, 169, 343, 350, 418, 423
Hpy188III TCNNGA 1 cut(s) 226
HpyAV CCTTC 1 cut(s) 421
HpyCH4III ACNGT 2 cut(s) 163, 356
HpyCH4V TGCA 3 cut(s) 4, 308, 381
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 407
Hsp92II CATG 7 cut(s) 22, 112, 169, 343, 350, 418, 423
KpnI GGTACC 1 cut(s) 32
Ksp22I TGATCA 1 cut(s) 261
Kzo9I GATC 2 cut(s) 35, 261
LmnI GCTCC 1 cut(s) 348
LpnPI CCDG 7 cut(s) 18, 45, 62, 67, 158, 234, 261
Lsp1109I GCAGC 4 cut(s) 292, 320, 388, 393
MaeI CTAG 2 cut(s) 302, 324
MaeIII GTNAC 3 cut(s) 157, 212, 459
MalI GATC 2 cut(s) 37, 263
MboI GATC 2 cut(s) 35, 261
MboII GAAGA 3 cut(s) 92, 97, 230
MhlI GDGCHC 2 cut(s) 225, 236
MlsI TGGCCA 1 cut(s) 17
MluNI TGGCCA 1 cut(s) 17
MmeI TCCRAC 1 cut(s) 253
MnlI CCTC 4 cut(s) 19, 100, 246, 378
Mox20I TGGCCA 1 cut(s) 17
MscI TGGCCA 1 cut(s) 17
Msp20I TGGCCA 1 cut(s) 17
MspR9I CCNGG 2 cut(s) 33, 249
MvaI CCWGG 2 cut(s) 33, 249
MwoI GCNNNNNNNGC 2 cut(s) 10, 407
NcoI CCATGG 1 cut(s) 18
NdeII GATC 2 cut(s) 35, 261
NlaIII CATG 7 cut(s) 22, 112, 169, 343, 350, 418, 423
NlaIV GGNNCC 1 cut(s) 30
PkrI GCNGC 4 cut(s) 307, 310, 383, 403
Psp6I CCWGG 2 cut(s) 31, 247
PspGI CCWGG 2 cut(s) 31, 247
PspN4I GGNNCC 1 cut(s) 30
PspPI GGNCC 1 cut(s) 356
PstI CTGCAG 1 cut(s) 310
RsaI GTAC 1 cut(s) 30
RsaNI GTAC 1 cut(s) 29
SatI GCNGC 4 cut(s) 306, 309, 382, 402
Sau3AI GATC 2 cut(s) 35, 261
Sau96I GGNCC 1 cut(s) 356
ScrFI CCNGG 2 cut(s) 33, 249
SduI GDGCHC 2 cut(s) 225, 236
SetI ASST 7 cut(s) 30, 34, 59, 137, 184, 307, 313
SfcI CTRYAG 2 cut(s) 130, 306
SinI GGWCC 1 cut(s) 356
SspMI CTAG 2 cut(s) 302, 324
StyD4I CCNGG 2 cut(s) 31, 247
StyI CCWWGG 2 cut(s) 18, 182
TaaI ACNGT 2 cut(s) 163, 356
TscAI CASTG 1 cut(s) 145
TseI GCWGC 4 cut(s) 305, 308, 381, 401
TspDTI ATGAA 2 cut(s) 97, 403
TspGWI ACGGA 1 cut(s) 456
TspRI CASTG 1 cut(s) 145
VpaK11BI GGWCC 1 cut(s) 356
XspI CTAG 2 cut(s) 302, 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.