Rw6G009820

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0015886
Physical Location & Seq
Forward (+)
16527574 .. 16529037
1464 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G009820.1

Sequence Viewer

Length: 462 bp
ATGGATCATGAGGTACCTGGCTCAGTGGGAACCAGTGCCAGCTTCTCTTTGAGATTGGGTCAGGCTATTTTTTCCTCTGCTTCACTTCTCTTCATGTCTTTGGGGGTTGAGTTCTACAGCTACACTGCTTTCTGCTACCTGGTCACAGTCATGGGTCTGGTTATACCATGGAGTTTCACATTGGCAATGGTTGATGGTTACTCTGTTCTTGTTAAATGCCCCCTTCGTCAGCCTGGAATACTGCTGATCATTGTTGTAGGAGATTGGATCCTATCAATTCTTACTCTGGGTGCAGCATGCTCAACTGCTAGTGTTGTGGACGTCCTGCTCCACGCAGGTGGTCCATATTGCCCTCCAAAGTTTTGCAGCAGGTACAAGATATCTGCTGCAATGGCTTTCATGACATGGTTTCTCTCGTTGGCTTCATCTCTTTTCAATCTCTGGTTACTTCCCTCCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

153

Amino Acids

16.52

Weight (kDa)

6.68

Isoelectric Point (pI)

32.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 9 - 138 3e-26 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015886)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49405
fragaria_vesca FvH4_2g07710
malus_domestica MD10G1084900.v1.1
prunus_persica Prupe.8G107800_v2.0.a1 Prupe.8G107800_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0262031
rosa_laevigata RLG00000014459
rosa_multiflora Rmu_sc0006019.1_g000002
rosa_roxburghii Rroxscaffold_7G00207760
rosa_rugosa Rorug05G0595100
rosa_samantha Rh6AG114500 Rh6BG108700 Rh6CG106000 Rh6DG096400
rosa_wichuraiana Rw6G009820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 326
AatII GACGTC 1 cut(s) 324
Acc36I ACCTGC 2 cut(s) 326, 360
Acc65I GGTACC 1 cut(s) 13
AccB1I GGYRCC 1 cut(s) 13
AclWI GGATC 3 cut(s) 12, 262, 275
AcyI GRCGYC 1 cut(s) 321
AfaI GTAC 2 cut(s) 15, 374
AgsI TTSAA 1 cut(s) 436
AjnI CCWGG 3 cut(s) 16, 138, 232
AleI CACNNNNGTG 1 cut(s) 336
AluBI AGCT 2 cut(s) 42, 120
AluI AGCT 2 cut(s) 42, 120
AlwI GGATC 3 cut(s) 12, 262, 275
ApeKI GCWGC 3 cut(s) 293, 366, 386
Asp718I GGTACC 1 cut(s) 13
AspS9I GGNCC 1 cut(s) 341
AvaII GGWCC 1 cut(s) 341
BamHI GGATCC 1 cut(s) 267
BanI GGYRCC 1 cut(s) 13
BbvI GCAGC 3 cut(s) 305, 373, 378
BccI CCATC 1 cut(s) 188
BciT130I CCWGG 3 cut(s) 18, 140, 234
BclI TGATCA 1 cut(s) 246
BfaI CTAG 1 cut(s) 309
BfmI CTRYAG 1 cut(s) 115
BfuAI ACCTGC 2 cut(s) 326, 360
BisI GCNGC 3 cut(s) 294, 367, 387
BlsI GCNGC 3 cut(s) 295, 368, 388
Bme1390I CCNGG 3 cut(s) 18, 140, 234
Bme18I GGWCC 1 cut(s) 341
BmgT120I GGNCC 1 cut(s) 341
BmiI GGNNCC 3 cut(s) 15, 31, 269
BmrFI CCNGG 3 cut(s) 18, 140, 234
BsaHI GRCGYC 1 cut(s) 321
BsaJI CCNNGG 1 cut(s) 167
Bse1I ACTGG 1 cut(s) 33
Bse3DI GCAATG 2 cut(s) 192, 396
BseBI CCWGG 3 cut(s) 18, 140, 234
BseDI CCNNGG 1 cut(s) 167
BseMI GCAATG 2 cut(s) 192, 396
BseMII CTCAG 1 cut(s) 36
BseNI ACTGG 1 cut(s) 33
BseXI GCAGC 3 cut(s) 305, 373, 378
BsgI GTGCAG 1 cut(s) 312
BshNI GGYRCC 1 cut(s) 13
Bsp143I GATC 3 cut(s) 4, 246, 267
Bsp19I CCATGG 1 cut(s) 167
BspCNI CTCAG 1 cut(s) 35
BspHI TCATGA 2 cut(s) 7, 399
BspLI GGNNCC 3 cut(s) 15, 31, 269
BspMI ACCTGC 2 cut(s) 326, 360
BspPI GGATC 3 cut(s) 12, 262, 275
BspT107I GGYRCC 1 cut(s) 13
BsrDI GCAATG 2 cut(s) 192, 396
BsrI ACTGG 1 cut(s) 33
BssECI CCNNGG 1 cut(s) 167
BssMI GATC 3 cut(s) 4, 246, 267
BssNI GRCGYC 1 cut(s) 321
BssT1I CCWWGG 1 cut(s) 167
Bst2UI CCWGG 3 cut(s) 18, 140, 234
Bst4CI ACNGT 1 cut(s) 148
Bst6I CTCTTC 1 cut(s) 95
BstACI GRCGYC 1 cut(s) 321
BstC8I GCNNGC 2 cut(s) 40, 298
BstDEI CTNAG 1 cut(s) 22
BstDSI CCRYGG 1 cut(s) 167
BstKTI GATC 3 cut(s) 7, 249, 270
BstMBI GATC 3 cut(s) 4, 246, 267
BstMWI GCNNNNNNNGC 1 cut(s) 392
BstNI CCWGG 3 cut(s) 18, 140, 234
BstNSI RCATGY 1 cut(s) 300
BstSCI CCNGG 3 cut(s) 16, 138, 232
BstSFI CTRYAG 1 cut(s) 115
BstV1I GCAGC 3 cut(s) 305, 373, 378
BstX2I RGATCY 1 cut(s) 267
BstXI CCANNNNNNTGG 1 cut(s) 338
BstYI RGATCY 1 cut(s) 267
BtgI CCRYGG 1 cut(s) 167
BtsI GCAGTG 1 cut(s) 123
BtsIMutI CAGTG 3 cut(s) 30, 40, 123
BveI ACCTGC 2 cut(s) 326, 360
Cac8I GCNNGC 2 cut(s) 40, 298
CciI TCATGA 2 cut(s) 7, 399
Cfr13I GGNCC 1 cut(s) 341
CsiI ACCWGGT 1 cut(s) 138
Csp6I GTAC 2 cut(s) 14, 373
CviAII CATG 7 cut(s) 8, 94, 151, 168, 297, 400, 405
CviJI RGCY 7 cut(s) 21, 42, 65, 120, 232, 395, 422
CviKI_1 RGCY 7 cut(s) 21, 42, 65, 120, 232, 395, 422
CviQI GTAC 2 cut(s) 14, 373
DdeI CTNAG 1 cut(s) 22
DpnI GATC 3 cut(s) 6, 248, 269
DpnII GATC 3 cut(s) 4, 246, 267
Eam1104I CTCTTC 1 cut(s) 95
EarI CTCTTC 1 cut(s) 95
Eco130I CCWWGG 1 cut(s) 167
Eco32I GATATC 1 cut(s) 381
Eco47I GGWCC 1 cut(s) 341
EcoRII CCWGG 3 cut(s) 16, 138, 232
EcoRV GATATC 1 cut(s) 381
EcoT14I CCWWGG 1 cut(s) 167
ErhI CCWWGG 1 cut(s) 167
FaeI CATG 7 cut(s) 11, 97, 154, 171, 300, 403, 408
FaiI YATR 9 cut(s) 9, 95, 152, 164, 169, 298, 346, 401, 406
FatI CATG 7 cut(s) 7, 93, 150, 167, 296, 399, 404
FbaI TGATCA 1 cut(s) 246
Fnu4HI GCNGC 3 cut(s) 294, 367, 387
Fsp4HI GCNGC 3 cut(s) 294, 367, 387
FspBI CTAG 1 cut(s) 309
GluI GCNGC 3 cut(s) 294, 367, 387
Hin1I GRCGYC 1 cut(s) 321
Hin1II CATG 7 cut(s) 11, 97, 154, 171, 300, 403, 408
Hpy166II GTNNAC 1 cut(s) 319
Hpy188III TCNNGA 2 cut(s) 8, 400
Hpy8I GTNNAC 1 cut(s) 319
HpyAV CCTTC 1 cut(s) 233
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 3 cut(s) 293, 366, 389
HpyF10VI GCNNNNNNNGC 1 cut(s) 392
HpyF3I CTNAG 1 cut(s) 22
HpySE526I ACGT 1 cut(s) 321
Hsp92I GRCGYC 1 cut(s) 321
Hsp92II CATG 7 cut(s) 11, 97, 154, 171, 300, 403, 408
KpnI GGTACC 1 cut(s) 17
Ksp22I TGATCA 1 cut(s) 246
Kzo9I GATC 3 cut(s) 4, 246, 267
LmnI GCTCC 1 cut(s) 333
Lsp1109I GCAGC 3 cut(s) 305, 373, 378
MabI ACCWGGT 1 cut(s) 138
MaeI CTAG 1 cut(s) 309
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 3 cut(s) 142, 197, 444
MalI GATC 3 cut(s) 6, 248, 269
MboI GATC 3 cut(s) 4, 246, 267
MboII GAAGA 1 cut(s) 82
MflI RGATCY 1 cut(s) 267
MluCI AATT 1 cut(s) 276
MnlI CCTC 3 cut(s) 4, 85, 363
MseI TTAA 1 cut(s) 213
MslI CAYNNNNRTG 2 cut(s) 149, 336
MspR9I CCNGG 3 cut(s) 18, 140, 234
MvaI CCWGG 3 cut(s) 18, 140, 234
MwoI GCNNNNNNNGC 1 cut(s) 392
NcoI CCATGG 1 cut(s) 167
NdeII GATC 3 cut(s) 4, 246, 267
NlaIII CATG 7 cut(s) 11, 97, 154, 171, 300, 403, 408
NlaIV GGNNCC 3 cut(s) 15, 31, 269
NmuCI GTSAC 1 cut(s) 142
NspI RCATGY 1 cut(s) 300
OliI CACNNNNGTG 1 cut(s) 336
PaeI GCATGC 1 cut(s) 300
PagI TCATGA 2 cut(s) 7, 399
PaqCI CACCTGC 1 cut(s) 326
PkrI GCNGC 3 cut(s) 295, 368, 388
Psp6I CCWGG 3 cut(s) 16, 138, 232
PspGI CCWGG 3 cut(s) 16, 138, 232
PspN4I GGNNCC 3 cut(s) 15, 31, 269
PspPI GGNCC 1 cut(s) 341
PsuI RGATCY 1 cut(s) 267
RsaI GTAC 2 cut(s) 15, 374
RsaNI GTAC 2 cut(s) 14, 373
RseI CAYNNNNRTG 2 cut(s) 149, 336
SaqAI TTAA 1 cut(s) 213
SatI GCNGC 3 cut(s) 294, 367, 387
Sau3AI GATC 3 cut(s) 4, 246, 267
Sau96I GGNCC 1 cut(s) 341
ScrFI CCNGG 3 cut(s) 18, 140, 234
SetI ASST 8 cut(s) 15, 19, 44, 122, 141, 324, 340, 374
SexAI ACCWGGT 1 cut(s) 138
SfcI CTRYAG 1 cut(s) 115
SinI GGWCC 1 cut(s) 341
SmiMI CAYNNNNRTG 2 cut(s) 149, 336
SphI GCATGC 1 cut(s) 300
Sse9I AATT 1 cut(s) 276
SspMI CTAG 1 cut(s) 309
StyD4I CCNGG 3 cut(s) 16, 138, 232
StyI CCWWGG 1 cut(s) 167
TaaI ACNGT 1 cut(s) 148
TaiI ACGT 1 cut(s) 324
TasI AATT 1 cut(s) 276
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TscAI CASTG 3 cut(s) 30, 40, 130
TseFI GTSAC 1 cut(s) 142
TseI GCWGC 3 cut(s) 293, 366, 386
Tsp45I GTSAC 1 cut(s) 142
TspDTI ATGAA 3 cut(s) 82, 388, 414
TspRI CASTG 3 cut(s) 30, 40, 130
VpaK11BI GGWCC 1 cut(s) 341
XceI RCATGY 1 cut(s) 300
XspI CTAG 1 cut(s) 309
ZraI GACGTC 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.