MD10G1136800.v1.1

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0019668
Physical Location & Seq
Reverse (-)
22138207 .. 22139308
1102 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1136800.v1.1.491

Sequence Viewer

Length: 549 bp
ATGGCGTTCGCTGCTTCTGATTCGAAAACTCATTCGAATCACTACTTGTTGATTGCTACTAGTGGAGGCTTGAACCAACAAAGAACAGGGATAACAGATGCTGTCGTTGCAGCTTATATCTTGAACGCTACTCTTGTTGTTCCCAAGCTGGACCAGAAATCGTATTGGAAGGATTCCAGCAACTTCGATGAAATCTTTGATGTAGACTGGTTTATATCATCTCTCTCAAAAGATGTTAAAATCATTAAGGAGCTTCCAACAAAGGGAGGGAAACCAATGAGTGTGCATACCACGCGAGTCCCAAGGAAGTGTAATGAAAAATGTTACCAGAACCGCTTGGTGCCCGTTCTTGATAAAAAGCATGCTGTTCAGCTCACAAAATTTGATTACAGACTTTCAAATAGGTTGGATTCAGATTTACAAAAGCTGAGATGTAGAGCCAATTATCAAGCCTTGAAATTTACCGACACAATTAGTGAAATGCGTAAAAAGTTGGTTGAGAGAATGAGAATGAAAAGCAAGCATTTCATTGCCCTACATCTGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

183

Amino Acids

20.98

Weight (kDa)

9.82

Isoelectric Point (pI)

24.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 15 - 182 3e-54 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019668)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g16370
malus_domestica MD10G1136800.v1.1
rosa_multiflora Rmu_co8180264.1_g000001 Rmu_sc0007472.1_g000012 Rmu_sc0009634.1_g000008 Rmu_sc0040469.1_g000001
rosa_samantha Rh7AG196300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 340
AccI GTMKAC 1 cut(s) 204
AccII CGCG 1 cut(s) 295
AciI CCGC 1 cut(s) 334
AcsI RAATTY 2 cut(s) 380, 458
AfiI CCNNNNNNNGG 1 cut(s) 263
AgsI TTSAA 4 cut(s) 73, 124, 399, 457
AhlI ACTAGT 1 cut(s) 59
AluBI AGCT 5 cut(s) 113, 148, 253, 373, 427
AluI AGCT 5 cut(s) 113, 148, 253, 373, 427
AlwNI CAGNNNCTG 1 cut(s) 101
ApeKI GCWGC 2 cut(s) 11, 110
ApoI RAATTY 2 cut(s) 380, 458
AspS9I GGNCC 1 cut(s) 151
AsuII TTCGAA 2 cut(s) 23, 35
AvaII GGWCC 1 cut(s) 151
BaeGI GKGCMC 1 cut(s) 345
BanI GGYRCC 1 cut(s) 340
BbvI GCAGC 1 cut(s) 122
BcuI ACTAGT 1 cut(s) 59
BfaI CTAG 1 cut(s) 60
BisI GCNGC 2 cut(s) 12, 111
BlsI GCNGC 2 cut(s) 13, 112
Bme18I GGWCC 1 cut(s) 151
BmgT120I GGNCC 1 cut(s) 151
BmiI GGNNCC 1 cut(s) 342
BmsI GCATC 1 cut(s) 88
Bpu14I TTCGAA 2 cut(s) 23, 35
BsaJI CCNNGG 1 cut(s) 302
Bsc4I CCNNNNNNNGG 1 cut(s) 263
Bse1I ACTGG 1 cut(s) 212
Bse3DI GCAATG 1 cut(s) 528
BseDI CCNNGG 1 cut(s) 302
BseLI CCNNNNNNNGG 1 cut(s) 263
BseMI GCAATG 1 cut(s) 528
BseMII CTCAG 2 cut(s) 419, 533
BseNI ACTGG 1 cut(s) 212
BseSI GKGCMC 1 cut(s) 345
BseXI GCAGC 1 cut(s) 122
Bsh1236I CGCG 1 cut(s) 295
BshNI GGYRCC 1 cut(s) 340
BslFI GGGAC 1 cut(s) 284
BslI CCNNNNNNNGG 1 cut(s) 263
BsmFI GGGAC 1 cut(s) 284
Bsp119I TTCGAA 2 cut(s) 23, 35
Bsp1286I GDGCHC 1 cut(s) 345
BspACI CCGC 1 cut(s) 334
BspCNI CTCAG 2 cut(s) 420, 534
BspFNI CGCG 1 cut(s) 295
BspLI GGNNCC 1 cut(s) 342
BspT104I TTCGAA 2 cut(s) 23, 35
BspT107I GGYRCC 1 cut(s) 340
BsrDI GCAATG 1 cut(s) 528
BsrI ACTGG 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 302
BssT1I CCWWGG 1 cut(s) 302
BstBI TTCGAA 2 cut(s) 23, 35
BstC8I GCNNGC 2 cut(s) 363, 521
BstDEI CTNAG 2 cut(s) 428, 542
BstFNI CGCG 1 cut(s) 295
BstMWI GCNNNNNNNGC 3 cut(s) 11, 107, 292
BstNSI RCATGY 1 cut(s) 365
BstSLI GKGCMC 1 cut(s) 345
BstUI CGCG 1 cut(s) 295
BstV1I GCAGC 1 cut(s) 122
Cac8I GCNNGC 2 cut(s) 363, 521
CaiI CAGNNNCTG 1 cut(s) 101
Cfr13I GGNCC 1 cut(s) 151
CviAII CATG 1 cut(s) 362
CviJI RGCY 8 cut(s) 69, 113, 148, 253, 373, 427, 440, 452
CviKI_1 RGCY 8 cut(s) 69, 113, 148, 253, 373, 427, 440, 452
DdeI CTNAG 2 cut(s) 428, 542
Eco130I CCWWGG 1 cut(s) 302
Eco47I GGWCC 1 cut(s) 151
EcoT14I CCWWGG 1 cut(s) 302
ErhI CCWWGG 1 cut(s) 302
FaeI CATG 1 cut(s) 365
FaiI YATR 4 cut(s) 117, 215, 288, 363
FaqI GGGAC 1 cut(s) 284
FatI CATG 1 cut(s) 361
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 2 cut(s) 12, 111
Fsp4HI GCNGC 2 cut(s) 12, 111
FspBI CTAG 1 cut(s) 60
GluI GCNGC 2 cut(s) 12, 111
Hin1II CATG 1 cut(s) 365
HinfI GANTC 5 cut(s) 20, 37, 173, 297, 410
Hpy166II GTNNAC 1 cut(s) 205
Hpy188I TCNGA 3 cut(s) 19, 415, 543
Hpy188III TCNNGA 2 cut(s) 121, 350
Hpy8I GTNNAC 1 cut(s) 205
HpyAV CCTTC 1 cut(s) 163
HpyCH4V TGCA 2 cut(s) 110, 286
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 107, 292
HpyF3I CTNAG 2 cut(s) 428, 542
Hsp92II CATG 1 cut(s) 365
LmnI GCTCC 1 cut(s) 250
LpnPI CCDG 6 cut(s) 72, 134, 167, 190, 193, 341
Lsp1109I GCAGC 1 cut(s) 122
LweI GCATC 1 cut(s) 88
MaeI CTAG 1 cut(s) 60
MaeIII GTNAC 1 cut(s) 323
MhlI GDGCHC 1 cut(s) 345
MluCI AATT 4 cut(s) 380, 442, 458, 471
MlyI GAGTC 1 cut(s) 306
MmeI TCCRAC 2 cut(s) 281, 387
MnlI CCTC 3 cut(s) 59, 260, 537
MseI TTAA 2 cut(s) 237, 246
MvnI CGCG 1 cut(s) 295
MwoI GCNNNNNNNGC 3 cut(s) 11, 107, 292
NlaIII CATG 1 cut(s) 365
NlaIV GGNNCC 1 cut(s) 342
NspI RCATGY 1 cut(s) 365
NspV TTCGAA 2 cut(s) 23, 35
PaeI GCATGC 1 cut(s) 365
PfeI GAWTC 4 cut(s) 20, 37, 173, 410
PkrI GCNGC 2 cut(s) 13, 112
PleI GAGTC 1 cut(s) 305
PpsI GAGTC 1 cut(s) 305
PspN4I GGNNCC 1 cut(s) 342
PspPI GGNCC 1 cut(s) 151
PstNI CAGNNNCTG 1 cut(s) 101
SaqAI TTAA 2 cut(s) 237, 246
SatI GCNGC 2 cut(s) 12, 111
Sau96I GGNCC 1 cut(s) 151
SchI GAGTC 1 cut(s) 306
SduI GDGCHC 1 cut(s) 345
SetI ASST 7 cut(s) 115, 150, 255, 375, 407, 429, 548
SfaNI GCATC 1 cut(s) 88
SfuI TTCGAA 2 cut(s) 23, 35
SinI GGWCC 1 cut(s) 151
SpeI ACTAGT 1 cut(s) 59
SphI GCATGC 1 cut(s) 365
Sse9I AATT 4 cut(s) 380, 442, 458, 471
SsiI CCGC 1 cut(s) 334
SspMI CTAG 1 cut(s) 60
StyI CCWWGG 1 cut(s) 302
TaqI TCGA 3 cut(s) 23, 35, 186
TasI AATT 4 cut(s) 380, 442, 458, 471
TfiI GAWTC 4 cut(s) 20, 37, 173, 410
Tru1I TTAA 2 cut(s) 237, 246
Tru9I TTAA 2 cut(s) 237, 246
TseI GCWGC 2 cut(s) 11, 110
TspDTI ATGAA 4 cut(s) 204, 330, 517, 527
VpaK11BI GGWCC 1 cut(s) 151
XapI RAATTY 2 cut(s) 380, 458
XceI RCATGY 1 cut(s) 365
XmiI GTMKAC 1 cut(s) 204
XspI CTAG 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.