MD10G1151000.v1.1

Pleckstrin homology domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0019738
Physical Location & Seq
Forward (+)
23757317 .. 23757568
252 bp
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UTR
Exon/CDS
Intron
MD10G1151000.v1.1.491

Sequence Viewer

Length: 252 bp
ATGACGCTCTCGTCGATGCTGCGCAGTGTGATCTCGATCGGCACGTGCCTGACTGTGAAGGGCGCCAAGGACGTGGTCCACAAGCCTTGCGCTTTCGAGCTGTCGACTACCAACCATGACACGATGTACTTCATTGCCGATTCAGAGAAAGAGAAGGAGGAATGGATTAACTCGATCGGAAGGTCCATAGTTCAGCACTTCAGATCGCTCGCGGATTCCGAGGTTGTCGATTACGATAGCAGCCGACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0032266 GO:0035091 GO:0043167 GO:0043168 GO:1901981
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PH

Protein Analysis

84

Amino Acids

9.34

Weight (kDa)

5.48

Isoelectric Point (pI)

46.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 8 - 63 1e-06 PH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019738)

Species Orthologous Gene IDs
malus_domestica MD10G1151000.v1.1
rosa_chinensis RchiOBHm_Chr6g0311721
rosa_multiflora Rmu_sc0000548.1_g000017 Rmu_sc0001836.1_g000013 Rmu_sc0003502.1_g000002 Rmu_sc0018663.1_g000001
rosa_samantha Rh3DG349600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 10
Acc16I TGCGCA 1 cut(s) 23
AccB1I GGYRCC 1 cut(s) 62
AccI GTMKAC 1 cut(s) 104
AccII CGCG 1 cut(s) 212
AciI CCGC 1 cut(s) 212
AcuI CTGAAG 1 cut(s) 184
AcvI CACGTG 1 cut(s) 45
AcyI GRCGYC 1 cut(s) 63
AfaI GTAC 1 cut(s) 128
AjiI CACGTC 1 cut(s) 73
AluBI AGCT 1 cut(s) 100
AluI AGCT 1 cut(s) 100
ApeKI GCWGC 2 cut(s) 19, 240
AspLEI GCGC 3 cut(s) 24, 65, 92
AspS9I GGNCC 2 cut(s) 76, 183
AvaII GGWCC 2 cut(s) 76, 183
BanI GGYRCC 1 cut(s) 62
BbrPI CACGTG 1 cut(s) 45
BbvI GCAGC 1 cut(s) 6
BfoI RGCGCY 1 cut(s) 66
BisI GCNGC 2 cut(s) 20, 241
BlsI GCNGC 2 cut(s) 21, 242
Bme18I GGWCC 2 cut(s) 76, 183
BmgBI CACGTC 1 cut(s) 73
BmgT120I GGNCC 2 cut(s) 76, 183
BmiI GGNNCC 1 cut(s) 64
BmsI GCATC 1 cut(s) 6
BsaAI YACGTR 1 cut(s) 45
BsaBI GATNNNNATC 1 cut(s) 35
BsaHI GRCGYC 1 cut(s) 63
BsaJI CCNNGG 2 cut(s) 66, 219
Bse3DI GCAATG 1 cut(s) 132
Bse8I GATNNNNATC 1 cut(s) 35
BseDI CCNNGG 2 cut(s) 66, 219
BseJI GATNNNNATC 1 cut(s) 35
BseMI GCAATG 1 cut(s) 132
BseXI GCAGC 1 cut(s) 6
Bsh1236I CGCG 1 cut(s) 212
Bsh1285I CGRYCG 2 cut(s) 39, 177
BshNI GGYRCC 1 cut(s) 62
BsiEI CGRYCG 2 cut(s) 39, 177
Bsp143I GATC 4 cut(s) 30, 36, 174, 203
BspACI CCGC 1 cut(s) 212
BspFNI CGCG 1 cut(s) 212
BspLI GGNNCC 1 cut(s) 64
BspT107I GGYRCC 1 cut(s) 62
BsrDI GCAATG 1 cut(s) 132
BssECI CCNNGG 2 cut(s) 66, 219
BssMI GATC 4 cut(s) 30, 36, 174, 203
BssNI GRCGYC 1 cut(s) 63
BssT1I CCWWGG 1 cut(s) 66
Bst4CI ACNGT 1 cut(s) 55
BstACI GRCGYC 1 cut(s) 63
BstBAI YACGTR 1 cut(s) 45
BstC8I GCNNGC 1 cut(s) 210
BstFNI CGCG 1 cut(s) 212
BstH2I RGCGCY 1 cut(s) 66
BstHHI GCGC 3 cut(s) 24, 65, 92
BstKTI GATC 4 cut(s) 33, 39, 177, 206
BstMBI GATC 4 cut(s) 30, 36, 174, 203
BstMCI CGRYCG 2 cut(s) 39, 177
BstUI CGCG 1 cut(s) 212
BstV1I GCAGC 1 cut(s) 6
BstXI CCANNNNNNTGG 1 cut(s) 73
BtrI CACGTC 1 cut(s) 73
BtsI GCAGTG 1 cut(s) 31
BtsIMutI CAGTG 1 cut(s) 31
Cac8I GCNNGC 1 cut(s) 210
CfoI GCGC 3 cut(s) 24, 65, 92
Cfr13I GGNCC 2 cut(s) 76, 183
CseI GACGC 1 cut(s) 13
Csp6I GTAC 1 cut(s) 127
CspCI CAANNNNNGTGG 2 cut(s) 68, 103
CviAII CATG 1 cut(s) 116
CviJI RGCY 3 cut(s) 85, 100, 243
CviKI_1 RGCY 3 cut(s) 85, 100, 243
CviQI GTAC 1 cut(s) 127
DinI GGCGCC 1 cut(s) 64
DpnI GATC 4 cut(s) 32, 38, 176, 205
DpnII GATC 4 cut(s) 30, 36, 174, 203
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
Eco130I CCWWGG 1 cut(s) 66
Eco47I GGWCC 2 cut(s) 76, 183
Eco57I CTGAAG 1 cut(s) 184
Eco72I CACGTG 1 cut(s) 45
EcoT14I CCWWGG 1 cut(s) 66
EgeI GGCGCC 1 cut(s) 64
EheI GGCGCC 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 66
FaeI CATG 1 cut(s) 119
FaiI YATR 2 cut(s) 117, 188
FatI CATG 1 cut(s) 115
FblI GTMKAC 1 cut(s) 104
Fnu4HI GCNGC 2 cut(s) 20, 241
Fsp4HI GCNGC 2 cut(s) 20, 241
FspI TGCGCA 1 cut(s) 23
GlaI GCGC 3 cut(s) 23, 64, 91
GluI GCNGC 2 cut(s) 20, 241
HaeII RGCGCY 1 cut(s) 66
HgaI GACGC 1 cut(s) 13
HhaI GCGC 3 cut(s) 24, 65, 92
Hin1I GRCGYC 1 cut(s) 63
Hin1II CATG 1 cut(s) 119
Hin6I GCGC 3 cut(s) 22, 63, 90
HinP1I GCGC 3 cut(s) 22, 63, 90
HincII GTYRAC 1 cut(s) 105
HindII GTYRAC 1 cut(s) 105
HinfI GANTC 2 cut(s) 140, 215
Hpy166II GTNNAC 2 cut(s) 79, 105
Hpy188I TCNGA 4 cut(s) 145, 179, 203, 220
Hpy188III TCNNGA 1 cut(s) 34
Hpy8I GTNNAC 2 cut(s) 79, 105
Hpy99I CGWCG 1 cut(s) 16
HpyAV CCTTC 3 cut(s) 52, 148, 174
HpyCH4III ACNGT 1 cut(s) 55
HpyCH4IV ACGT 2 cut(s) 44, 72
HpySE526I ACGT 2 cut(s) 44, 72
Hsp92I GRCGYC 1 cut(s) 63
Hsp92II CATG 1 cut(s) 119
HspAI GCGC 3 cut(s) 22, 63, 90
KasI GGCGCC 1 cut(s) 62
Kzo9I GATC 4 cut(s) 30, 36, 174, 203
LpnPI CCDG 1 cut(s) 62
Lsp1109I GCAGC 1 cut(s) 6
LweI GCATC 1 cut(s) 6
MaeII ACGT 2 cut(s) 44, 72
MalI GATC 4 cut(s) 32, 38, 176, 205
MboI GATC 4 cut(s) 30, 36, 174, 203
Mly113I GGCGCC 1 cut(s) 63
MnlI CCTC 2 cut(s) 151, 214
MseI TTAA 1 cut(s) 168
MvnI CGCG 1 cut(s) 212
NarI GGCGCC 1 cut(s) 63
NdeII GATC 4 cut(s) 30, 36, 174, 203
NlaIII CATG 1 cut(s) 119
NlaIV GGNNCC 1 cut(s) 64
NsbI TGCGCA 1 cut(s) 23
PcsI WCGNNNNNNNCGW 3 cut(s) 11, 41, 216
PfeI GAWTC 2 cut(s) 140, 215
PflFI GACNNNGTC 1 cut(s) 74
PkrI GCNGC 2 cut(s) 21, 242
Ple19I CGATCG 2 cut(s) 39, 177
PluTI GGCGCC 1 cut(s) 66
PmaCI CACGTG 1 cut(s) 45
PmlI CACGTG 1 cut(s) 45
Ppu21I YACGTR 1 cut(s) 45
PspCI CACGTG 1 cut(s) 45
PspN4I GGNNCC 1 cut(s) 64
PspPI GGNCC 2 cut(s) 76, 183
PsyI GACNNNGTC 1 cut(s) 74
PvuI CGATCG 2 cut(s) 39, 177
RsaI GTAC 1 cut(s) 128
RsaNI GTAC 1 cut(s) 127
SalI GTCGAC 1 cut(s) 103
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 2 cut(s) 20, 241
Sau3AI GATC 4 cut(s) 30, 36, 174, 203
Sau96I GGNCC 2 cut(s) 76, 183
SetI ASST 5 cut(s) 47, 75, 102, 185, 225
SfaNI GCATC 1 cut(s) 6
SfoI GGCGCC 1 cut(s) 64
SinI GGWCC 2 cut(s) 76, 183
SsiI CCGC 1 cut(s) 212
SspDI GGCGCC 1 cut(s) 62
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 1 cut(s) 55
TaiI ACGT 2 cut(s) 47, 75
TaqI TCGA 6 cut(s) 14, 35, 96, 104, 173, 228
TatI WGTACW 1 cut(s) 126
TfiI GAWTC 2 cut(s) 140, 215
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TscAI CASTG 1 cut(s) 31
TseI GCWGC 2 cut(s) 19, 240
TspDTI ATGAA 1 cut(s) 121
TspRI CASTG 1 cut(s) 31
Tth111I GACNNNGTC 1 cut(s) 74
VpaK11BI GGWCC 2 cut(s) 76, 183
XmiI GTMKAC 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.