MD10G1162700.v1.1

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0019737
Physical Location & Seq
Forward (+)
25335832 .. 25337261
1430 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1162700.v1.1.491

Sequence Viewer

Length: 744 bp
ATGAAGCCACAGGGGTTGAAACTGAATACACTGTACTTTTATCGATGCGGGGATCCTTCTATACATGCAATGAGTCAAATCCACCATTTCAGGACTATCCCAGTTTCTGGTCCTCGGAGCTACCCTGAGAGAATAGCGGTTATGGGAGACCTTGGCCTTACTTACAACACAACTACCACAATAAGTCACTTGAAAAGTAACAATCCTGATCTTGTTCTATTGATTGGTGATGTTACTTATGCAAACCTCTACCTCACAAATGGAACTGGCTCTGACTGCTATTCTTGCTCATTTCCAAACTCTCCAATACATGAGACCTATCAGCCTCGATGGGATTACTGGGGATGGTATATGCAGAGTTTAGTGTCCAAAGTTCCAATAATGGTTGTTCATGCCTACAAGAGATCGAATCGAGATTATGATTACAACTTAGATTCCTGTGGTCCTGTGTATCTCACCGTTGGTGACGGGGGTAATCGGGAGAAGATGGCAGTTCAACATGCTGACGAACCTGGTCATTGCCCAGAGCCATCAACTACTCCTGATCAACACCTAGGTGCTGGTGCCTTTTGTGTAGAGAAATTTACCTCCGGCCCAGCAGCAGGTAAATTTTGTTGGGATCGGCAACCGAATTACAGTGCTTTCAGAGAAAGCAGCTTTGGCCATGGGATCCTTGAGGTACTCCTTTTTGTGTTTTTATGTTTGGCATATATCCAGTCTGACAATGCATTCAATTGTCTATAA

Protein Analysis

248

Amino Acids

27.69

Weight (kDa)

6.18

Isoelectric Point (pI)

43.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019737)

Species Orthologous Gene IDs
malus_domestica MD10G1162700.v1.1
pyrus_communis pycom10g14010
rosa_multiflora Rmu_sc0000635.1_g000002 Rmu_sc0003748.1_g000015
rosa_roxburghii Rroxscaffold_3G00235640 Rroxscaffold_5G00358910 Rroxscaffold_7G00184610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 593
AccB1I GGYRCC 1 cut(s) 563
AccB7I CCANNNNNTGG 1 cut(s) 107
AciI CCGC 2 cut(s) 48, 137
AclWI GGATC 5 cut(s) 47, 60, 627, 664, 677
AcoI YGGCCR 1 cut(s) 661
AcsI RAATTY 2 cut(s) 581, 608
AfaI GTAC 2 cut(s) 35, 681
AfiI CCNNNNNNNGG 2 cut(s) 107, 602
AgsI TTSAA 4 cut(s) 19, 193, 497, 733
AjnI CCWGG 1 cut(s) 511
AjuI GAANNNNNNNTTGG 2 cut(s) 642, 674
AleI CACNNNNGTG 1 cut(s) 555
AluBI AGCT 2 cut(s) 120, 657
AluI AGCT 2 cut(s) 120, 657
Alw26I GTCTC 2 cut(s) 141, 308
AlwI GGATC 5 cut(s) 47, 60, 627, 664, 677
AlwNI CAGNNNCTG 1 cut(s) 107
AoxI GGCC 3 cut(s) 154, 592, 661
ApeKI GCWGC 2 cut(s) 599, 654
ApoI RAATTY 2 cut(s) 581, 608
AspA2I CCTAGG 1 cut(s) 553
AspS9I GGNCC 3 cut(s) 110, 443, 593
AsuHPI GGTGA 3 cut(s) 239, 448, 476
AvaII GGWCC 2 cut(s) 110, 443
AvrII CCTAGG 1 cut(s) 553
BalI TGGCCA 1 cut(s) 663
BamHI GGATCC 2 cut(s) 52, 669
BanI GGYRCC 1 cut(s) 563
BbvI GCAGC 2 cut(s) 611, 666
BccI CCATC 4 cut(s) 324, 339, 481, 538
BciT130I CCWGG 1 cut(s) 513
BclI TGATCA 1 cut(s) 544
BcoDI GTCTC 2 cut(s) 141, 308
BfaI CTAG 1 cut(s) 554
BfuAI ACCTGC 1 cut(s) 593
BisI GCNGC 2 cut(s) 600, 655
BlnI CCTAGG 1 cut(s) 553
BlsI GCNGC 2 cut(s) 601, 656
Bme1390I CCNGG 1 cut(s) 513
Bme18I GGWCC 2 cut(s) 110, 443
BmgT120I GGNCC 3 cut(s) 110, 443, 593
BmiI GGNNCC 3 cut(s) 54, 565, 671
BmrFI CCNGG 1 cut(s) 513
BmrI ACTGGG 2 cut(s) 95, 349
BmsI GCATC 1 cut(s) 35
BmuI ACTGGG 2 cut(s) 95, 349
BpuEI CTTGAG 1 cut(s) 695
Bsa29I ATCGAT 1 cut(s) 43
BsaI GGTCTC 2 cut(s) 141, 308
BsaJI CCNNGG 4 cut(s) 113, 151, 553, 664
Bsc4I CCNNNNNNNGG 2 cut(s) 107, 602
Bse1I ACTGG 4 cut(s) 101, 271, 344, 715
Bse3DI GCAATG 2 cut(s) 75, 517
BseBI CCWGG 1 cut(s) 513
BseCI ATCGAT 1 cut(s) 43
BseDI CCNNGG 4 cut(s) 113, 151, 553, 664
BseGI GGATG 1 cut(s) 350
BseLI CCNNNNNNNGG 2 cut(s) 107, 602
BseMI GCAATG 2 cut(s) 75, 517
BseMII CTCAG 1 cut(s) 117
BseNI ACTGG 4 cut(s) 101, 271, 344, 715
BseXI GCAGC 2 cut(s) 611, 666
BseYI CCCAGC 1 cut(s) 595
BshFI GGCC 3 cut(s) 156, 594, 663
BshNI GGYRCC 1 cut(s) 563
BshVI ATCGAT 1 cut(s) 43
BsiSI CCGG 1 cut(s) 591
BslI CCNNNNNNNGG 2 cut(s) 107, 602
BsmAI GTCTC 2 cut(s) 141, 308
BsmI GAATGC 1 cut(s) 728
BsnI GGCC 3 cut(s) 156, 594, 663
Bso31I GGTCTC 2 cut(s) 141, 308
Bsp143I GATC 6 cut(s) 52, 208, 404, 544, 619, 669
Bsp19I CCATGG 1 cut(s) 664
BspACI CCGC 2 cut(s) 48, 137
BspANI GGCC 3 cut(s) 156, 594, 663
BspCNI CTCAG 1 cut(s) 118
BspDI ATCGAT 1 cut(s) 43
BspLI GGNNCC 3 cut(s) 54, 565, 671
BspMI ACCTGC 1 cut(s) 593
BspPI GGATC 5 cut(s) 47, 60, 627, 664, 677
BspT107I GGYRCC 1 cut(s) 563
BspTNI GGTCTC 2 cut(s) 141, 308
BsrDI GCAATG 2 cut(s) 75, 517
BsrI ACTGG 4 cut(s) 101, 271, 344, 715
BssECI CCNNGG 4 cut(s) 113, 151, 553, 664
BssMI GATC 6 cut(s) 52, 208, 404, 544, 619, 669
BssT1I CCWWGG 3 cut(s) 151, 553, 664
Bst2UI CCWGG 1 cut(s) 513
Bst4CI ACNGT 3 cut(s) 33, 460, 638
BstDEI CTNAG 2 cut(s) 126, 430
BstDSI CCRYGG 1 cut(s) 664
BstF5I GGATG 1 cut(s) 350
BstKTI GATC 6 cut(s) 55, 211, 407, 547, 622, 672
BstMAI GTCTC 2 cut(s) 141, 308
BstMBI GATC 6 cut(s) 52, 208, 404, 544, 619, 669
BstMWI GCNNNNNNNGC 3 cut(s) 276, 285, 660
BstNI CCWGG 1 cut(s) 513
BstNSI RCATGY 2 cut(s) 68, 503
BstSCI CCNGG 1 cut(s) 511
BstV1I GCAGC 2 cut(s) 611, 666
BstX2I RGATCY 2 cut(s) 52, 669
BstYI RGATCY 2 cut(s) 52, 669
Bsu15I ATCGAT 1 cut(s) 43
BsuRI GGCC 3 cut(s) 156, 594, 663
BsuTUI ATCGAT 1 cut(s) 43
BtgI CCRYGG 1 cut(s) 664
BtsCI GGATG 1 cut(s) 350
BtsIMutI CAGTG 2 cut(s) 29, 643
BveI ACCTGC 1 cut(s) 593
CaiI CAGNNNCTG 1 cut(s) 107
Cfr13I GGNCC 3 cut(s) 110, 443, 593
ClaI ATCGAT 1 cut(s) 43
CsiI ACCWGGT 1 cut(s) 511
Csp6I GTAC 2 cut(s) 34, 680
CspCI CAANNNNNGTGG 1 cut(s) 32
CviAII CATG 5 cut(s) 65, 311, 392, 500, 665
CviJI RGCY 9 cut(s) 7, 120, 156, 270, 325, 529, 594, 657, 663
CviKI_1 RGCY 9 cut(s) 7, 120, 156, 270, 325, 529, 594, 657, 663
CviQI GTAC 2 cut(s) 34, 680
DdeI CTNAG 2 cut(s) 126, 430
DpnI GATC 6 cut(s) 54, 210, 406, 546, 621, 671
DpnII GATC 6 cut(s) 52, 208, 404, 544, 619, 669
EaeI YGGCCR 1 cut(s) 661
Eco130I CCWWGG 3 cut(s) 151, 553, 664
Eco31I GGTCTC 2 cut(s) 141, 308
Eco47I GGWCC 2 cut(s) 110, 443
EcoRII CCWGG 1 cut(s) 511
EcoT14I CCWWGG 3 cut(s) 151, 553, 664
EcoT22I ATGCAT 1 cut(s) 730
ErhI CCWWGG 3 cut(s) 151, 553, 664
FaeI CATG 5 cut(s) 68, 314, 395, 503, 668
FatI CATG 5 cut(s) 64, 310, 391, 499, 664
FauI CCCGC 1 cut(s) 41
FbaI TGATCA 1 cut(s) 544
Fnu4HI GCNGC 2 cut(s) 600, 655
FokI GGATG 1 cut(s) 357
Fsp4HI GCNGC 2 cut(s) 600, 655
FspBI CTAG 1 cut(s) 554
GluI GCNGC 2 cut(s) 600, 655
GsaI CCCAGC 1 cut(s) 599
HaeIII GGCC 3 cut(s) 156, 594, 663
HapII CCGG 1 cut(s) 591
Hin1II CATG 5 cut(s) 68, 314, 395, 503, 668
HinfI GANTC 3 cut(s) 73, 409, 434
HpaII CCGG 1 cut(s) 591
HphI GGTGA 3 cut(s) 239, 448, 476
Hpy188I TCNGA 4 cut(s) 117, 274, 647, 721
Hpy188III TCNNGA 5 cut(s) 91, 206, 413, 479, 542
HpyAV CCTTC 1 cut(s) 66
HpyCH4III ACNGT 3 cut(s) 33, 460, 638
HpyCH4V TGCA 4 cut(s) 68, 242, 355, 728
HpyF10VI GCNNNNNNNGC 3 cut(s) 276, 285, 660
HpyF3I CTNAG 2 cut(s) 126, 430
Hsp92II CATG 5 cut(s) 68, 314, 395, 503, 668
Ksp22I TGATCA 1 cut(s) 544
Kzo9I GATC 6 cut(s) 52, 208, 404, 544, 619, 669
LmnI GCTCC 1 cut(s) 117
Lsp1109I GCAGC 2 cut(s) 611, 666
LweI GCATC 1 cut(s) 35
MabI ACCWGGT 1 cut(s) 511
MaeI CTAG 1 cut(s) 554
MaeIII GTNAC 4 cut(s) 185, 197, 232, 464
MalI GATC 6 cut(s) 54, 210, 406, 546, 621, 671
MboI GATC 6 cut(s) 52, 208, 404, 544, 619, 669
MboII GAAGA 1 cut(s) 496
MfeI CAATTG 1 cut(s) 733
MflI RGATCY 2 cut(s) 52, 669
MlsI TGGCCA 1 cut(s) 663
MluCI AATT 4 cut(s) 581, 608, 631, 733
MluNI TGGCCA 1 cut(s) 663
MlyI GAGTC 1 cut(s) 82
MnlI CCTC 6 cut(s) 123, 257, 263, 336, 598, 670
Mox20I TGGCCA 1 cut(s) 663
Mph1103I ATGCAT 1 cut(s) 730
MscI TGGCCA 1 cut(s) 663
MslI CAYNNNNRTG 1 cut(s) 555
Msp20I TGGCCA 1 cut(s) 663
MspI CCGG 1 cut(s) 591
MspR9I CCNGG 1 cut(s) 513
MunI CAATTG 1 cut(s) 733
Mva1269I GAATGC 1 cut(s) 728
MvaI CCWGG 1 cut(s) 513
MwoI GCNNNNNNNGC 3 cut(s) 276, 285, 660
NcoI CCATGG 1 cut(s) 664
NdeII GATC 6 cut(s) 52, 208, 404, 544, 619, 669
NlaIII CATG 5 cut(s) 68, 314, 395, 503, 668
NlaIV GGNNCC 3 cut(s) 54, 565, 671
NmuCI GTSAC 2 cut(s) 185, 464
NsiI ATGCAT 1 cut(s) 730
NspI RCATGY 2 cut(s) 68, 503
OliI CACNNNNGTG 1 cut(s) 555
PctI GAATGC 1 cut(s) 728
PfeI GAWTC 2 cut(s) 409, 434
PflMI CCANNNNNTGG 1 cut(s) 107
PkrI GCNGC 2 cut(s) 601, 656
PleI GAGTC 1 cut(s) 81
PpsI GAGTC 1 cut(s) 81
Psp6I CCWGG 1 cut(s) 511
PspFI CCCAGC 1 cut(s) 595
PspGI CCWGG 1 cut(s) 511
PspN4I GGNNCC 3 cut(s) 54, 565, 671
PspPI GGNCC 3 cut(s) 110, 443, 593
PstNI CAGNNNCTG 1 cut(s) 107
PsuI RGATCY 2 cut(s) 52, 669
RsaI GTAC 2 cut(s) 35, 681
RsaNI GTAC 2 cut(s) 34, 680
RseI CAYNNNNRTG 1 cut(s) 555
SatI GCNGC 2 cut(s) 600, 655
Sau3AI GATC 6 cut(s) 52, 208, 404, 544, 619, 669
Sau96I GGNCC 3 cut(s) 110, 443, 593
SchI GAGTC 1 cut(s) 82
ScrFI CCNGG 1 cut(s) 513
SexAI ACCWGGT 1 cut(s) 511
SfaNI GCATC 1 cut(s) 35
SinI GGWCC 2 cut(s) 110, 443
SmiMI CAYNNNNRTG 1 cut(s) 555
SmlI CTYRAG 1 cut(s) 674
SmoI CTYRAG 1 cut(s) 674
Sse9I AATT 4 cut(s) 581, 608, 631, 733
SsiI CCGC 2 cut(s) 48, 137
SspMI CTAG 1 cut(s) 554
StyD4I CCNGG 1 cut(s) 511
StyI CCWWGG 3 cut(s) 151, 553, 664
TaaI ACNGT 3 cut(s) 33, 460, 638
TaqI TCGA 4 cut(s) 43, 328, 407, 412
TasI AATT 4 cut(s) 581, 608, 631, 733
TatI WGTACW 1 cut(s) 33
TfiI GAWTC 2 cut(s) 409, 434
TscAI CASTG 2 cut(s) 36, 643
TseFI GTSAC 2 cut(s) 185, 464
TseI GCWGC 2 cut(s) 599, 654
Tsp45I GTSAC 2 cut(s) 185, 464
TspDTI ATGAA 2 cut(s) 17, 380
TspRI CASTG 2 cut(s) 36, 643
Van91I CCANNNNNTGG 1 cut(s) 107
VpaK11BI GGWCC 2 cut(s) 110, 443
XapI RAATTY 2 cut(s) 581, 608
XceI RCATGY 2 cut(s) 68, 503
XmaJI CCTAGG 1 cut(s) 553
XspI CTAG 1 cut(s) 554
Zsp2I ATGCAT 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.