Rroxscaffold_5G00358910

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0019737
Physical Location & Seq
Forward (+)
38989951 .. 38992302
2352 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00358910.1

Sequence Viewer

Length: 723 bp
ATGGAACACACCACTCCTTCCCCCAACCAATCCAGTGTGTTGAGAAGGCTCACGCGATATGTCCGTTTACCCCTAGGTGCCGGTTCATACTTAGCCCGTGCGAACGGTAATGGACTTGGGGCGGCGGTTAGGATAGTTAGTCGGAAACTTAATGTCAAAGAAATAGAGGCCATCGTGGTAAGGTGTGCCGCTGCTCCGACAATCACCGCTCTCATCGTGGACGTGCTTCTCGTACATGCGCACATAGATGGTGTCCGGAGACCATTCTCCAAAATTTTCCACTCACGCATGATCTTCTTGTATACGATCGCCACCGTTGGTGAAACAATCTCCATCTCCGCTTTTGTTGTTCTTCTTCCCCCTGCCCTTGCTTTTCCTCCTTACCGTAGTTGTGATTTTGGTGATTGGGATGCTGCTTTTGAATCGTCGTGGACAACTGGAATAACTGGAGTGAATCCACCCACAACGCCATTGCCGAAGGTGGGATTGGTCGTTGTCGTCGCTGGTGGACTTGGAATGGTTATAGTTGTGATCGATCGGAGTTGTGCCTCTAAAGGTAACTGTGAGATGATGGAAATTGCACATGCGGATGAACCTGGAAACTGCCCGCAACCATCTACGATGCCTGGTCATTATATGGGGGGTTTCTGTGCACTTAATTTCACTCCAGGCCGAGCAGCAGGAAAGTTCTGTTGGGATGAGCAACCCGACTATAGTGCATAG

Protein Analysis

240

Amino Acids

25.69

Weight (kDa)

8.18

Isoelectric Point (pI)

43.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019737)

Species Orthologous Gene IDs
malus_domestica MD10G1162700.v1.1
pyrus_communis pycom10g14010
rosa_multiflora Rmu_sc0000635.1_g000002 Rmu_sc0003748.1_g000015
rosa_roxburghii Rroxscaffold_3G00235640 Rroxscaffold_5G00358910 Rroxscaffold_7G00184610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 240
AccB1I GGYRCC 1 cut(s) 77
AccBSI CCGCTC 1 cut(s) 209
AccI GTMKAC 1 cut(s) 302
AccII CGCG 1 cut(s) 55
AccIII TCCGGA 1 cut(s) 255
AciI CCGC 7 cut(s) 122, 125, 189, 207, 339, 587, 608
AcsI RAATTY 1 cut(s) 273
AfaI GTAC 1 cut(s) 234
AfiI CCNNNNNNNGG 1 cut(s) 482
AgsI TTSAA 1 cut(s) 422
AjiI CACGTC 1 cut(s) 223
AjnI CCWGG 3 cut(s) 595, 625, 667
AjuI GAANNNNNNNTTGG 4 cut(s) 470, 502, 676, 708
Alw21I GWGCWC 1 cut(s) 655
Alw26I GTCTC 1 cut(s) 253
Alw44I GTGCAC 1 cut(s) 651
Aor13HI TCCGGA 1 cut(s) 255
AoxI GGCC 2 cut(s) 168, 670
ApaLI GTGCAC 1 cut(s) 651
ApeKI GCWGC 3 cut(s) 191, 413, 677
ApoI RAATTY 1 cut(s) 273
AspA2I CCTAGG 1 cut(s) 73
AspLEI GCGC 1 cut(s) 241
AsuHPI GGTGA 3 cut(s) 196, 332, 413
AvrII CCTAGG 1 cut(s) 73
BaeGI GKGCMC 1 cut(s) 655
BanI GGYRCC 1 cut(s) 77
Bbv12I GWGCWC 1 cut(s) 655
BbvI GCAGC 3 cut(s) 178, 400, 689
BccI CCATC 5 cut(s) 179, 242, 341, 565, 622
BcgI CGANNNNNNTGC 1 cut(s) 698
BciT130I CCWGG 3 cut(s) 597, 627, 669
BcoDI GTCTC 1 cut(s) 253
BfaI CTAG 1 cut(s) 74
BfmI CTRYAG 1 cut(s) 712
BisI GCNGC 5 cut(s) 123, 189, 192, 414, 678
BlnI CCTAGG 1 cut(s) 73
BlsI GCNGC 5 cut(s) 124, 190, 193, 415, 679
Bme1390I CCNGG 3 cut(s) 597, 627, 669
BmgBI CACGTC 1 cut(s) 223
BmiI GGNNCC 1 cut(s) 79
BmrFI CCNGG 3 cut(s) 597, 627, 669
BmsI GCATC 2 cut(s) 400, 612
BpmI CTGGAG 2 cut(s) 468, 651
Bsa29I ATCGAT 1 cut(s) 534
BsaI GGTCTC 1 cut(s) 253
BsaJI CCNNGG 1 cut(s) 73
BsaWI WCCGGW 1 cut(s) 255
Bsc4I CCNNNNNNNGG 1 cut(s) 482
Bse118I RCCGGY 1 cut(s) 80
Bse1I ACTGG 3 cut(s) 33, 442, 451
Bse3DI GCAATG 1 cut(s) 470
BseAI TCCGGA 1 cut(s) 255
BseBI CCWGG 3 cut(s) 597, 627, 669
BseCI ATCGAT 1 cut(s) 534
BseDI CCNNGG 1 cut(s) 73
BseGI GGATG 3 cut(s) 415, 595, 703
BseLI CCNNNNNNNGG 1 cut(s) 482
BseMI GCAATG 1 cut(s) 470
BseNI ACTGG 3 cut(s) 33, 442, 451
BseSI GKGCMC 1 cut(s) 655
BseXI GCAGC 3 cut(s) 178, 400, 689
Bsh1236I CGCG 1 cut(s) 55
Bsh1285I CGRYCG 2 cut(s) 309, 538
BshFI GGCC 2 cut(s) 170, 672
BshNI GGYRCC 1 cut(s) 77
BshVI ATCGAT 1 cut(s) 534
BsiEI CGRYCG 2 cut(s) 309, 538
BsiHKAI GWGCWC 1 cut(s) 655
BsiSI CCGG 2 cut(s) 81, 256
BslI CCNNNNNNNGG 1 cut(s) 482
BsmAI GTCTC 1 cut(s) 253
BsnI GGCC 2 cut(s) 170, 672
Bso31I GGTCTC 1 cut(s) 253
Bsp1286I GDGCHC 1 cut(s) 655
Bsp13I TCCGGA 1 cut(s) 255
Bsp143I GATC 4 cut(s) 291, 306, 531, 535
BspACI CCGC 7 cut(s) 122, 125, 189, 207, 339, 587, 608
BspANI GGCC 2 cut(s) 170, 672
BspDI ATCGAT 1 cut(s) 534
BspEI TCCGGA 1 cut(s) 255
BspFNI CGCG 1 cut(s) 55
BspLI GGNNCC 1 cut(s) 79
BspT107I GGYRCC 1 cut(s) 77
BspTNI GGTCTC 1 cut(s) 253
BsrBI CCGCTC 1 cut(s) 209
BsrDI GCAATG 1 cut(s) 470
BsrFI RCCGGY 1 cut(s) 80
BsrI ACTGG 3 cut(s) 33, 442, 451
BssAI RCCGGY 1 cut(s) 80
BssECI CCNNGG 1 cut(s) 73
BssMI GATC 4 cut(s) 291, 306, 531, 535
BssNAI GTATAC 1 cut(s) 303
BssT1I CCWWGG 1 cut(s) 73
Bst1107I GTATAC 1 cut(s) 303
Bst2UI CCWGG 3 cut(s) 597, 627, 669
Bst4CI ACNGT 4 cut(s) 107, 316, 386, 563
BstC8I GCNNGC 1 cut(s) 608
BstDEI CTNAG 1 cut(s) 91
BstF5I GGATG 3 cut(s) 415, 595, 703
BstFNI CGCG 1 cut(s) 55
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 4 cut(s) 294, 309, 534, 538
BstMAI GTCTC 1 cut(s) 253
BstMBI GATC 4 cut(s) 291, 306, 531, 535
BstMCI CGRYCG 2 cut(s) 309, 538
BstNI CCWGG 3 cut(s) 597, 627, 669
BstNSI RCATGY 2 cut(s) 239, 587
BstSCI CCNGG 3 cut(s) 595, 625, 667
BstSFI CTRYAG 1 cut(s) 712
BstSLI GKGCMC 1 cut(s) 655
BstUI CGCG 1 cut(s) 55
BstV1I GCAGC 3 cut(s) 178, 400, 689
BstZ17I GTATAC 1 cut(s) 303
Bsu15I ATCGAT 1 cut(s) 534
BsuRI GGCC 2 cut(s) 170, 672
BsuTUI ATCGAT 1 cut(s) 534
BtrI CACGTC 1 cut(s) 223
BtsCI GGATG 3 cut(s) 415, 595, 703
BtsIMutI CAGTG 1 cut(s) 40
Cac8I GCNNGC 1 cut(s) 608
CfoI GCGC 1 cut(s) 241
Cfr10I RCCGGY 1 cut(s) 80
ClaI ATCGAT 1 cut(s) 534
Csp6I GTAC 1 cut(s) 233
CviAII CATG 3 cut(s) 236, 289, 584
CviJI RGCY 4 cut(s) 49, 95, 170, 672
CviKI_1 RGCY 4 cut(s) 49, 95, 170, 672
CviQI GTAC 1 cut(s) 233
DdeI CTNAG 1 cut(s) 91
DpnI GATC 4 cut(s) 293, 308, 533, 537
DpnII GATC 4 cut(s) 291, 306, 531, 535
Eco130I CCWWGG 1 cut(s) 73
Eco31I GGTCTC 1 cut(s) 253
EcoRII CCWGG 3 cut(s) 595, 625, 667
EcoT14I CCWWGG 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 73
FaeI CATG 3 cut(s) 239, 292, 587
FatI CATG 3 cut(s) 235, 288, 583
FauI CCCGC 1 cut(s) 615
FblI GTMKAC 1 cut(s) 302
Fnu4HI GCNGC 5 cut(s) 123, 189, 192, 414, 678
FokI GGATG 3 cut(s) 422, 602, 710
Fsp4HI GCNGC 5 cut(s) 123, 189, 192, 414, 678
FspAI RTGCGCAY 1 cut(s) 240
FspBI CTAG 1 cut(s) 74
FspI TGCGCA 1 cut(s) 240
GlaI GCGC 1 cut(s) 240
GluI GCNGC 5 cut(s) 123, 189, 192, 414, 678
GsuI CTGGAG 2 cut(s) 468, 651
HaeIII GGCC 2 cut(s) 170, 672
HapII CCGG 2 cut(s) 81, 256
HhaI GCGC 1 cut(s) 241
Hin1II CATG 3 cut(s) 239, 292, 587
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HinfI GANTC 2 cut(s) 422, 454
HpaII CCGG 2 cut(s) 81, 256
HphI GGTGA 3 cut(s) 196, 332, 413
Hpy166II GTNNAC 6 cut(s) 68, 220, 303, 432, 509, 653
Hpy188I TCNGA 3 cut(s) 144, 198, 540
Hpy188III TCNNGA 1 cut(s) 256
Hpy8I GTNNAC 6 cut(s) 68, 220, 303, 432, 509, 653
Hpy99I CGWCG 2 cut(s) 430, 503
HpyAV CCTTC 3 cut(s) 27, 39, 472
HpyCH4III ACNGT 4 cut(s) 107, 316, 386, 563
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 3 cut(s) 581, 653, 719
HpyF3I CTNAG 1 cut(s) 91
HpySE526I ACGT 1 cut(s) 222
Hsp92II CATG 3 cut(s) 239, 292, 587
HspAI GCGC 1 cut(s) 239
Kpn2I TCCGGA 1 cut(s) 255
Kzo9I GATC 4 cut(s) 291, 306, 531, 535
LmnI GCTCC 1 cut(s) 199
Lsp1109I GCAGC 3 cut(s) 178, 400, 689
LweI GCATC 2 cut(s) 400, 612
MaeI CTAG 1 cut(s) 74
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 1 cut(s) 557
MalI GATC 4 cut(s) 293, 308, 533, 537
MbiI CCGCTC 1 cut(s) 209
MboI GATC 4 cut(s) 291, 306, 531, 535
MboII GAAGA 3 cut(s) 286, 344, 347
MhlI GDGCHC 1 cut(s) 655
MluCI AATT 3 cut(s) 273, 576, 658
MmeI TCCRAC 2 cut(s) 122, 221
MnlI CCTC 3 cut(s) 160, 387, 559
MroI TCCGGA 1 cut(s) 255
MseI TTAA 2 cut(s) 150, 657
MslI CAYNNNNRTG 2 cut(s) 246, 588
MspA1I CMGCKG 1 cut(s) 191
MspI CCGG 2 cut(s) 81, 256
MspR9I CCNGG 3 cut(s) 597, 627, 669
MvaI CCWGG 3 cut(s) 597, 627, 669
MvnI CGCG 1 cut(s) 55
NdeII GATC 4 cut(s) 291, 306, 531, 535
NlaIII CATG 3 cut(s) 239, 292, 587
NlaIV GGNNCC 1 cut(s) 79
NmeAIII GCCGAG 1 cut(s) 698
NsbI TGCGCA 1 cut(s) 240
NspI RCATGY 2 cut(s) 239, 587
PcsI WCGNNNNNNNCGW 2 cut(s) 228, 473
PfeI GAWTC 2 cut(s) 422, 454
PkrI GCNGC 5 cut(s) 124, 190, 193, 415, 679
Ple19I CGATCG 2 cut(s) 309, 538
Psp6I CCWGG 3 cut(s) 595, 625, 667
PspGI CCWGG 3 cut(s) 595, 625, 667
PspN4I GGNNCC 1 cut(s) 79
PvuI CGATCG 2 cut(s) 309, 538
RsaI GTAC 1 cut(s) 234
RsaNI GTAC 1 cut(s) 233
RseI CAYNNNNRTG 2 cut(s) 246, 588
SaqAI TTAA 2 cut(s) 150, 657
SatI GCNGC 5 cut(s) 123, 189, 192, 414, 678
Sau3AI GATC 4 cut(s) 291, 306, 531, 535
ScrFI CCNGG 3 cut(s) 597, 627, 669
SduI GDGCHC 1 cut(s) 655
SetI ASST 6 cut(s) 79, 185, 225, 483, 559, 598
SfaNI GCATC 2 cut(s) 400, 612
SfcI CTRYAG 1 cut(s) 712
SmiMI CAYNNNNRTG 2 cut(s) 246, 588
Sse9I AATT 3 cut(s) 273, 576, 658
SsiI CCGC 7 cut(s) 122, 125, 189, 207, 339, 587, 608
SspMI CTAG 1 cut(s) 74
StyD4I CCNGG 3 cut(s) 595, 625, 667
StyI CCWWGG 1 cut(s) 73
TaaI ACNGT 4 cut(s) 107, 316, 386, 563
TaiI ACGT 1 cut(s) 225
TaqI TCGA 1 cut(s) 534
TasI AATT 3 cut(s) 273, 576, 658
TauI GCSGC 2 cut(s) 125, 191
TfiI GAWTC 2 cut(s) 422, 454
Tru1I TTAA 2 cut(s) 150, 657
Tru9I TTAA 2 cut(s) 150, 657
TscAI CASTG 1 cut(s) 40
TseI GCWGC 3 cut(s) 191, 413, 677
TspDTI ATGAA 2 cut(s) 75, 606
TspGWI ACGGA 1 cut(s) 53
TspRI CASTG 1 cut(s) 40
VneI GTGCAC 1 cut(s) 651
XapI RAATTY 1 cut(s) 273
XceI RCATGY 2 cut(s) 239, 587
XmaJI CCTAGG 1 cut(s) 73
XmiI GTMKAC 1 cut(s) 302
XspI CTAG 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.