Rroxscaffold_3G00235640

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0019737
Physical Location & Seq
Forward (+)
22710043 .. 22711433
1391 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00235640.1

Sequence Viewer

Length: 609 bp
ATGGTGCATGCTTATGAGCGGATGAATAGAGTATATAACTATACGCTAGATCCATGTGGACCTGTTTATATAACAGTTGGAGATGGTGGCAATATAGAGAAAGTTGATGTTGATTTTGCTGATGACCCTGGAAAGTGTCCTTCAGCTGGAGACAGCATACCTGAATTTGGAGGGGTATGTCATCTGAACTTTTCTTCTGGGCCTGCTAAAGGGAAGTTTTGTTGGAATGAACAACCAGAGTGGAGTGCTTTTAGAGAAAGCAGCTTTGGTCATGGAATACTTGAGGTTTTGAATTCTACATATGCTCTATGGACTTGGCACAGGAGTCAAGATATTTATAAGGAAGATAGCCACGGCGATCAAATCTACATTGTTCGACAACCTGAATTCATTATTCCTACACTTATGCTCACTGTTATTATCTTTCTCAAACTGCCAGCCGTCAGTGGTGCAGTCTCTGCCCTCAATCTCTGCTCCTGCATCCCACTCTCTGGTGCTTGCTCTCTCCCTGACACTCTTGGGGATATTCTCAGTCCTCTCCTATTCCAAGTAGTCTCTTCTGCTCTGCAACTGTGCCTCTGCTTCTTCAGCTCACCTGTGAAATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

202

Amino Acids

22.21

Weight (kDa)

4.84

Isoelectric Point (pI)

45.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos_C PF14008 72 - 111 3e-11 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019737)

Species Orthologous Gene IDs
malus_domestica MD10G1162700.v1.1
pyrus_communis pycom10g14010
rosa_multiflora Rmu_sc0000635.1_g000002 Rmu_sc0003748.1_g000015
rosa_roxburghii Rroxscaffold_3G00235640 Rroxscaffold_5G00358910 Rroxscaffold_7G00184610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 339
AccB7I CCANNNNNTGG 1 cut(s) 491
AccBSI CCGCTC 1 cut(s) 19
AciI CCGC 1 cut(s) 19
AclWI GGATC 1 cut(s) 44
AcsI RAATTY 3 cut(s) 164, 292, 386
AcuI CTGAAG 2 cut(s) 126, 571
AfiI CCNNNNNNNGG 4 cut(s) 146, 167, 209, 491
AgsI TTSAA 1 cut(s) 292
AjnI CCWGG 1 cut(s) 127
AjuI GAANNNNNNNTTGG 2 cut(s) 249, 281
AluBI AGCT 3 cut(s) 146, 264, 591
AluI AGCT 3 cut(s) 146, 264, 591
Alw26I GTCTC 3 cut(s) 144, 460, 559
AlwI GGATC 1 cut(s) 44
AlwNI CAGNNNCTG 1 cut(s) 458
AoxI GGCC 1 cut(s) 200
ApeKI GCWGC 1 cut(s) 261
ApoI RAATTY 3 cut(s) 164, 292, 386
AspS9I GGNCC 2 cut(s) 59, 200
AsuHPI GGTGA 1 cut(s) 585
AvaII GGWCC 1 cut(s) 59
BbvI GCAGC 1 cut(s) 273
BccI CCATC 1 cut(s) 77
BceAI ACGGC 2 cut(s) 370, 425
BciT130I CCWGG 1 cut(s) 129
BcoDI GTCTC 3 cut(s) 144, 460, 559
BfaI CTAG 1 cut(s) 47
BisI GCNGC 1 cut(s) 262
BlsI GCNGC 1 cut(s) 263
Bme1390I CCNGG 1 cut(s) 129
Bme18I GGWCC 1 cut(s) 59
BmgT120I GGNCC 2 cut(s) 59, 200
BmrFI CCNGG 1 cut(s) 129
BmsI GCATC 1 cut(s) 489
BpmI CTGGAG 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 302
BsaJI CCNNGG 2 cut(s) 127, 352
Bsc4I CCNNNNNNNGG 4 cut(s) 146, 167, 209, 491
BseBI CCWGG 1 cut(s) 129
BseDI CCNNGG 2 cut(s) 127, 352
BseGI GGATG 2 cut(s) 27, 480
BseLI CCNNNNNNNGG 4 cut(s) 146, 167, 209, 491
BseMII CTCAG 1 cut(s) 544
BseXI GCAGC 1 cut(s) 273
BsgI GTGCAG 1 cut(s) 471
BshFI GGCC 1 cut(s) 202
BslI CCNNNNNNNGG 4 cut(s) 146, 167, 209, 491
BsmAI GTCTC 3 cut(s) 144, 460, 559
BsnI GGCC 1 cut(s) 202
Bsp143I GATC 2 cut(s) 49, 358
BspACI CCGC 1 cut(s) 19
BspANI GGCC 1 cut(s) 202
BspCNI CTCAG 1 cut(s) 543
BspPI GGATC 1 cut(s) 44
BsrBI CCGCTC 1 cut(s) 19
BssECI CCNNGG 2 cut(s) 127, 352
BssMI GATC 2 cut(s) 49, 358
Bst2UI CCWGG 1 cut(s) 129
Bst4CI ACNGT 3 cut(s) 76, 415, 573
Bst6I CTCTTC 1 cut(s) 562
BstAPI GCANNNNNTGC 1 cut(s) 458
BstC8I GCNNGC 4 cut(s) 9, 204, 438, 499
BstDEI CTNAG 1 cut(s) 530
BstDSI CCRYGG 1 cut(s) 352
BstENI CCTNNNNNAGG 1 cut(s) 207
BstF5I GGATG 2 cut(s) 27, 480
BstKTI GATC 2 cut(s) 52, 361
BstMAI GTCTC 3 cut(s) 144, 460, 559
BstMBI GATC 2 cut(s) 49, 358
BstMWI GCNNNNNNNGC 2 cut(s) 458, 588
BstNI CCWGG 1 cut(s) 129
BstNSI RCATGY 1 cut(s) 11
BstSCI CCNGG 1 cut(s) 127
BstV1I GCAGC 1 cut(s) 273
BstX2I RGATCY 1 cut(s) 49
BstYI RGATCY 1 cut(s) 49
BsuRI GGCC 1 cut(s) 202
BtgI CCRYGG 1 cut(s) 352
BtsCI GGATG 2 cut(s) 27, 480
BtsIMutI CAGTG 2 cut(s) 411, 451
Cac8I GCNNGC 4 cut(s) 9, 204, 438, 499
CaiI CAGNNNCTG 1 cut(s) 458
Cfr13I GGNCC 2 cut(s) 59, 200
CspCI CAANNNNNGTGG 2 cut(s) 221, 256
CviAII CATG 3 cut(s) 8, 54, 272
CviJI RGCY 6 cut(s) 146, 202, 264, 351, 440, 591
CviKI_1 RGCY 6 cut(s) 146, 202, 264, 351, 440, 591
DdeI CTNAG 1 cut(s) 530
DpnI GATC 2 cut(s) 51, 360
DpnII GATC 2 cut(s) 49, 358
Eam1104I CTCTTC 1 cut(s) 562
EarI CTCTTC 1 cut(s) 562
Eco47I GGWCC 1 cut(s) 59
Eco57I CTGAAG 2 cut(s) 126, 571
EcoNI CCTNNNNNAGG 1 cut(s) 207
EcoRI GAATTC 2 cut(s) 292, 386
EcoRII CCWGG 1 cut(s) 127
FaeI CATG 3 cut(s) 11, 57, 275
FatI CATG 3 cut(s) 7, 53, 271
FauNDI CATATG 1 cut(s) 301
Fnu4HI GCNGC 1 cut(s) 262
FokI GGATG 2 cut(s) 34, 467
Fsp4HI GCNGC 1 cut(s) 262
FspBI CTAG 1 cut(s) 47
GluI GCNGC 1 cut(s) 262
GsuI CTGGAG 1 cut(s) 168
HaeIII GGCC 1 cut(s) 202
Hin1II CATG 3 cut(s) 11, 57, 275
HinfI GANTC 1 cut(s) 325
HphI GGTGA 1 cut(s) 585
Hpy166II GTNNAC 1 cut(s) 59
Hpy188I TCNGA 1 cut(s) 186
Hpy188III TCNNGA 1 cut(s) 329
Hpy8I GTNNAC 1 cut(s) 59
HpyAV CCTTC 1 cut(s) 150
HpyCH4III ACNGT 3 cut(s) 76, 415, 573
HpyCH4V TGCA 4 cut(s) 7, 452, 480, 568
HpyF10VI GCNNNNNNNGC 2 cut(s) 458, 588
HpyF3I CTNAG 1 cut(s) 530
Hsp92II CATG 3 cut(s) 11, 57, 275
Kzo9I GATC 2 cut(s) 49, 358
LmnI GCTCC 1 cut(s) 479
Lsp1109I GCAGC 1 cut(s) 273
LweI GCATC 1 cut(s) 489
MaeI CTAG 1 cut(s) 47
MalI GATC 2 cut(s) 51, 360
MbiI CCGCTC 1 cut(s) 19
MboI GATC 2 cut(s) 49, 358
MboII GAAGA 4 cut(s) 186, 356, 549, 577
MflI RGATCY 1 cut(s) 49
MluCI AATT 3 cut(s) 164, 292, 386
MlyI GAGTC 1 cut(s) 334
MmeI TCCRAC 2 cut(s) 58, 203
MnlI CCTC 5 cut(s) 164, 277, 473, 546, 587
MseI TTAA 1 cut(s) 607
MslI CAYNNNNRTG 1 cut(s) 12
MspA1I CMGCKG 1 cut(s) 146
MspR9I CCNGG 1 cut(s) 129
MvaI CCWGG 1 cut(s) 129
MwoI GCNNNNNNNGC 2 cut(s) 458, 588
NdeI CATATG 1 cut(s) 301
NdeII GATC 2 cut(s) 49, 358
NlaIII CATG 3 cut(s) 11, 57, 275
NspI RCATGY 1 cut(s) 11
PaeI GCATGC 1 cut(s) 11
PflMI CCANNNNNTGG 1 cut(s) 491
PkrI GCNGC 1 cut(s) 263
PleI GAGTC 1 cut(s) 333
PpsI GAGTC 1 cut(s) 333
PsiI TTATAA 1 cut(s) 339
Psp6I CCWGG 1 cut(s) 127
PspGI CCWGG 1 cut(s) 127
PspPI GGNCC 2 cut(s) 59, 200
PstNI CAGNNNCTG 1 cut(s) 458
PsuI RGATCY 1 cut(s) 49
PvuII CAGCTG 1 cut(s) 146
RseI CAYNNNNRTG 1 cut(s) 12
SaqAI TTAA 1 cut(s) 607
SatI GCNGC 1 cut(s) 262
Sau3AI GATC 2 cut(s) 49, 358
Sau96I GGNCC 2 cut(s) 59, 200
SchI GAGTC 1 cut(s) 334
ScrFI CCNGG 1 cut(s) 129
SetI ASST 8 cut(s) 64, 148, 163, 266, 288, 385, 593, 598
SfaNI GCATC 1 cut(s) 489
SinI GGWCC 1 cut(s) 59
SmiMI CAYNNNNRTG 1 cut(s) 12
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
SphI GCATGC 1 cut(s) 11
Sse9I AATT 3 cut(s) 164, 292, 386
SsiI CCGC 1 cut(s) 19
SspI AATATT 1 cut(s) 605
SspMI CTAG 1 cut(s) 47
StyD4I CCNGG 1 cut(s) 127
TaaI ACNGT 3 cut(s) 76, 415, 573
TaqI TCGA 1 cut(s) 376
TasI AATT 3 cut(s) 164, 292, 386
Tru1I TTAA 1 cut(s) 607
Tru9I TTAA 1 cut(s) 607
TscAI CASTG 2 cut(s) 418, 451
TseI GCWGC 1 cut(s) 261
TspDTI ATGAA 3 cut(s) 38, 243, 379
TspRI CASTG 2 cut(s) 418, 451
Van91I CCANNNNNTGG 1 cut(s) 491
VpaK11BI GGWCC 1 cut(s) 59
XagI CCTNNNNNAGG 1 cut(s) 207
XapI RAATTY 3 cut(s) 164, 292, 386
XceI RCATGY 1 cut(s) 11
XspI CTAG 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.