MD10G1205200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013488
Physical Location & Seq
Reverse (-)
30429956 .. 30431474
1519 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1205200.v1.1.491

Sequence Viewer

Length: 759 bp
ATGGCTGCTGATGTGAGCTCTCTGTTTCGGGTTCTTAGTGGGTACAGCGACGACCAACATCTGAGCGTTGGGAGTGATTCTAGTGGTGAGAAATCAACGGCTCTGATTACAAGAGACTTGCTTGGTGGTAGTGGGTCGTCTTCTTCCAAGCTTCCCAACGACTCCCAGGAACTGGACCTCGACTTACAGGTCCCCAATGGCTGGGAAAAGCGCTTGGACTTGAAGTCAGGGAAAGTGTATCTTCAAAGGTGCAGTGCCCAAAATTCACATTCAAACTCAGATCAAAAGAGCCAAACCAATCGAACAGTTCCAAAGATGCAAGATTTGAACTTCCCTCCATCGGCGTCGTCGAAAATCCCACTAAATCTCTTTGATGAAACCAGCACTAACCTTGATTTGAAGTTAGTTTCATCATCACCACCACCCTCATGCAATTACCAGAGCCTATGCACTTTGGACAAGGTAAAATCCGCCCTCGAAAGAGCAGAGAAGGAGCCGATCAAGAGGCGGTCTTCGTCCATGTTGAAGTCATCGATGTCATCTTCGCCAACTTACTCATCGTCGTCGTCACCCATCAAAGAAACTCAGGAGGAGGACAGTGAGGACAAGCAATTTGCATCACCATTTGCAGCAGGGTGCCCTGGTTGCTTATCTTATGTACTGATCATGAGAAATAACCCTAAATGTCCTCGGTGCAATTCGGTTGTTCCGCTGCCAACGGTGAAGAAACCTCGGATCGATCTCAACAGATCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

253

Amino Acids

27.43

Weight (kDa)

8.41

Isoelectric Point (pI)

62.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn-ribbon_GIR1 PF24747 211 - 237 1.2e-07 GIR1-like zinc ribbon domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013488)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79160
fragaria_vesca FvH4_3g15360
malus_domestica MD05G1223700.v1.1 MD10G1205200.v1.1
prunus_persica Prupe.4G136500_v2.0.a1
pyrus_communis pycom05g20660 pycom10g17570
rosa_chinensis RchiOBHm_Chr5g0025801
rosa_laevigata RLG00000032876
rosa_multiflora Rmu_sc0005610.1_g000005
rosa_roxburghii Rroxscaffold_1G00053400 Rroxscaffold_1G00054340
rosa_rugosa Rorug05G0088600
rosa_samantha Rh5AG180700 Rh5BG179300 Rh5CG197100 Rh5DG179000
rosa_wichuraiana Rw5G016390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 188
AccB1I GGYRCC 1 cut(s) 636
AccB7I CCANNNNNTGG 2 cut(s) 172, 201
AciI CCGC 3 cut(s) 471, 508, 710
AclWI GGATC 1 cut(s) 743
AcsI RAATTY 1 cut(s) 262
AcyI GRCGYC 1 cut(s) 344
AfaI GTAC 2 cut(s) 44, 660
AfeI AGCGCT 1 cut(s) 212
AfiI CCNNNNNNNGG 3 cut(s) 172, 201, 340
AgsI TTSAA 6 cut(s) 223, 245, 273, 328, 400, 526
AhdI GACNNNNNGTC 1 cut(s) 223
AjnI CCWGG 2 cut(s) 165, 640
AluBI AGCT 2 cut(s) 18, 151
AluI AGCT 2 cut(s) 18, 151
Alw21I GWGCWC 1 cut(s) 20
Alw26I GTCTC 1 cut(s) 108
AlwI GGATC 1 cut(s) 743
AlwNI CAGNNNCTG 1 cut(s) 172
Aor51HI AGCGCT 1 cut(s) 212
ApeKI GCWGC 3 cut(s) 5, 629, 712
ApoI RAATTY 1 cut(s) 262
ArsI GACNNNNNNTTYG 2 cut(s) 596, 628
AspLEI GCGC 1 cut(s) 213
AspS9I GGNCC 2 cut(s) 175, 190
AsuHPI GGTGA 5 cut(s) 98, 408, 561, 612, 733
AvaII GGWCC 2 cut(s) 175, 190
BaeGI GKGCMC 2 cut(s) 259, 641
BanI GGYRCC 1 cut(s) 636
BanII GRGCYC 1 cut(s) 20
BbsI GAAGAC 2 cut(s) 132, 504
Bbv12I GWGCWC 1 cut(s) 20
BbvI GCAGC 2 cut(s) 641, 699
BccI CCATC 2 cut(s) 346, 581
BceAI ACGGC 1 cut(s) 114
BciT130I CCWGG 2 cut(s) 167, 642
BclI TGATCA 1 cut(s) 663
BcoDI GTCTC 1 cut(s) 108
BfaI CTAG 1 cut(s) 81
BfoI RGCGCY 1 cut(s) 214
BisI GCNGC 3 cut(s) 6, 630, 713
BlsI GCNGC 3 cut(s) 7, 631, 714
Bme1390I CCNGG 2 cut(s) 167, 642
Bme18I GGWCC 2 cut(s) 175, 190
BmeRI GACNNNNNGTC 1 cut(s) 223
BmgT120I GGNCC 2 cut(s) 175, 190
BmiI GGNNCC 3 cut(s) 192, 495, 638
BmrFI CCNGG 2 cut(s) 167, 642
BmsI GCATC 2 cut(s) 306, 626
BpiI GAAGAC 2 cut(s) 132, 504
Bsa29I ATCGAT 2 cut(s) 533, 738
BsaHI GRCGYC 1 cut(s) 344
BsaJI CCNNGG 4 cut(s) 165, 640, 689, 731
Bsc4I CCNNNNNNNGG 3 cut(s) 172, 201, 340
Bse1I ACTGG 1 cut(s) 177
BseBI CCWGG 2 cut(s) 167, 642
BseCI ATCGAT 2 cut(s) 533, 738
BseDI CCNNGG 4 cut(s) 165, 640, 689, 731
BseLI CCNNNNNNNGG 3 cut(s) 172, 201, 340
BseMII CTCAG 3 cut(s) 53, 291, 599
BseNI ACTGG 1 cut(s) 177
BseRI GAGGAG 1 cut(s) 605
BseSI GKGCMC 2 cut(s) 259, 641
BseXI GCAGC 2 cut(s) 641, 699
BseYI CCCAGC 1 cut(s) 201
BsgI GTGCAG 1 cut(s) 271
BshNI GGYRCC 1 cut(s) 636
BshVI ATCGAT 2 cut(s) 533, 738
BsiHKAI GWGCWC 1 cut(s) 20
BslFI GGGAC 1 cut(s) 176
BslI CCNNNNNNNGG 3 cut(s) 172, 201, 340
BsmAI GTCTC 1 cut(s) 108
BsmFI GGGAC 1 cut(s) 176
Bsp1286I GDGCHC 3 cut(s) 20, 259, 641
Bsp143I GATC 6 cut(s) 280, 498, 663, 735, 739, 749
BspACI CCGC 3 cut(s) 471, 508, 710
BspCNI CTCAG 3 cut(s) 54, 290, 598
BspDI ATCGAT 2 cut(s) 533, 738
BspHI TCATGA 1 cut(s) 666
BspLI GGNNCC 3 cut(s) 192, 495, 638
BspPI GGATC 1 cut(s) 743
BspT107I GGYRCC 1 cut(s) 636
BsrI ACTGG 1 cut(s) 177
BssECI CCNNGG 4 cut(s) 165, 640, 689, 731
BssMI GATC 6 cut(s) 280, 498, 663, 735, 739, 749
BssNI GRCGYC 1 cut(s) 344
Bst2UI CCWGG 2 cut(s) 167, 642
Bst4CI ACNGT 3 cut(s) 307, 599, 721
BstACI GRCGYC 1 cut(s) 344
BstDEI CTNAG 4 cut(s) 35, 62, 277, 585
BstH2I RGCGCY 1 cut(s) 214
BstHHI GCGC 1 cut(s) 213
BstKTI GATC 6 cut(s) 283, 501, 666, 738, 742, 752
BstMAI GTCTC 1 cut(s) 108
BstMBI GATC 6 cut(s) 280, 498, 663, 735, 739, 749
BstMWI GCNNNNNNNGC 1 cut(s) 645
BstNI CCWGG 2 cut(s) 167, 642
BstSCI CCNGG 2 cut(s) 165, 640
BstSLI GKGCMC 2 cut(s) 259, 641
BstV1I GCAGC 2 cut(s) 641, 699
BstV2I GAAGAC 2 cut(s) 132, 504
Bsu15I ATCGAT 2 cut(s) 533, 738
BsuTUI ATCGAT 2 cut(s) 533, 738
BtsI GCAGTG 1 cut(s) 259
BtsIMutI CAGTG 2 cut(s) 259, 604
CaiI CAGNNNCTG 1 cut(s) 172
CciI TCATGA 1 cut(s) 666
CfoI GCGC 1 cut(s) 213
Cfr13I GGNCC 2 cut(s) 175, 190
ClaI ATCGAT 2 cut(s) 533, 738
CseI GACGC 1 cut(s) 333
Csp6I GTAC 2 cut(s) 43, 659
CviAII CATG 3 cut(s) 429, 520, 667
CviJI RGCY 8 cut(s) 5, 18, 101, 151, 201, 291, 444, 496
CviKI_1 RGCY 8 cut(s) 5, 18, 101, 151, 201, 291, 444, 496
CviQI GTAC 2 cut(s) 43, 659
DdeI CTNAG 4 cut(s) 35, 62, 277, 585
DpnI GATC 6 cut(s) 282, 500, 665, 737, 741, 751
DpnII GATC 6 cut(s) 280, 498, 663, 735, 739, 749
DrdI GACNNNNNNGTC 1 cut(s) 188
DriI GACNNNNNGTC 1 cut(s) 223
DseDI GACNNNNNNGTC 1 cut(s) 188
Eam1105I GACNNNNNGTC 1 cut(s) 223
EciI GGCGGA 1 cut(s) 460
Ecl136II GAGCTC 1 cut(s) 18
Eco24I GRGCYC 1 cut(s) 20
Eco47I GGWCC 2 cut(s) 175, 190
Eco47III AGCGCT 1 cut(s) 212
Eco53kI GAGCTC 1 cut(s) 18
EcoICRI GAGCTC 1 cut(s) 18
EcoO109I RGGNCCY 1 cut(s) 190
EcoRII CCWGG 2 cut(s) 165, 640
EcoT38I GRGCYC 1 cut(s) 20
FaeI CATG 3 cut(s) 432, 523, 670
FaiI YATR 5 cut(s) 430, 448, 521, 657, 668
FalI AAGNNNNNCTT 2 cut(s) 225, 257
FaqI GGGAC 1 cut(s) 176
FatI CATG 3 cut(s) 428, 519, 666
FbaI TGATCA 1 cut(s) 663
Fnu4HI GCNGC 3 cut(s) 6, 630, 713
FriOI GRGCYC 1 cut(s) 20
Fsp4HI GCNGC 3 cut(s) 6, 630, 713
FspBI CTAG 1 cut(s) 81
GlaI GCGC 1 cut(s) 212
GluI GCNGC 3 cut(s) 6, 630, 713
GsaI CCCAGC 1 cut(s) 205
HaeII RGCGCY 1 cut(s) 214
HgaI GACGC 1 cut(s) 333
HhaI GCGC 1 cut(s) 213
Hin1I GRCGYC 1 cut(s) 344
Hin1II CATG 3 cut(s) 432, 523, 670
Hin6I GCGC 1 cut(s) 211
HinP1I GCGC 1 cut(s) 211
HindIII AAGCTT 1 cut(s) 149
HinfI GANTC 2 cut(s) 77, 161
HphI GGTGA 5 cut(s) 98, 408, 561, 612, 733
Hpy188I TCNGA 4 cut(s) 63, 105, 280, 735
Hpy188III TCNNGA 3 cut(s) 502, 587, 667
Hpy99I CGWCG 5 cut(s) 53, 349, 352, 565, 568
HpyAV CCTTC 1 cut(s) 484
HpyCH4III ACNGT 3 cut(s) 307, 599, 721
HpyCH4V TGCA 7 cut(s) 252, 319, 432, 450, 617, 629, 696
HpyF10VI GCNNNNNNNGC 1 cut(s) 645
HpyF3I CTNAG 4 cut(s) 35, 62, 277, 585
Hsp92I GRCGYC 1 cut(s) 344
Hsp92II CATG 3 cut(s) 432, 523, 670
HspAI GCGC 1 cut(s) 211
Ksp22I TGATCA 1 cut(s) 663
Kzo9I GATC 6 cut(s) 280, 498, 663, 735, 739, 749
LmnI GCTCC 1 cut(s) 493
Lsp1109I GCAGC 2 cut(s) 641, 699
LweI GCATC 2 cut(s) 306, 626
MaeI CTAG 1 cut(s) 81
MaeIII GTNAC 1 cut(s) 567
MalI GATC 6 cut(s) 282, 500, 665, 737, 741, 751
MboI GATC 6 cut(s) 280, 498, 663, 735, 739, 749
MboII GAAGA 6 cut(s) 132, 135, 233, 504, 534, 736
MhlI GDGCHC 3 cut(s) 20, 259, 641
MluCI AATT 5 cut(s) 262, 433, 611, 697, 754
MlyI GAGTC 1 cut(s) 155
MslI CAYNNNNRTG 1 cut(s) 427
MspA1I CMGCKG 1 cut(s) 712
MspR9I CCNGG 2 cut(s) 167, 642
MvaI CCWGG 2 cut(s) 167, 642
MwoI GCNNNNNNNGC 1 cut(s) 645
NdeII GATC 6 cut(s) 280, 498, 663, 735, 739, 749
NlaIII CATG 3 cut(s) 432, 523, 670
NlaIV GGNNCC 3 cut(s) 192, 495, 638
NmuCI GTSAC 1 cut(s) 567
PagI TCATGA 1 cut(s) 666
PcsI WCGNNNNNNNCGW 1 cut(s) 347
PfeI GAWTC 1 cut(s) 77
PflMI CCANNNNNTGG 2 cut(s) 172, 201
PkrI GCNGC 3 cut(s) 7, 631, 714
PleI GAGTC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 155
PpuMI RGGWCCY 1 cut(s) 190
Psp124BI GAGCTC 1 cut(s) 20
Psp5II RGGWCCY 1 cut(s) 190
Psp6I CCWGG 2 cut(s) 165, 640
PspFI CCCAGC 1 cut(s) 201
PspGI CCWGG 2 cut(s) 165, 640
PspN4I GGNNCC 3 cut(s) 192, 495, 638
PspPI GGNCC 2 cut(s) 175, 190
PspPPI RGGWCCY 1 cut(s) 190
PstNI CAGNNNCTG 1 cut(s) 172
RsaI GTAC 2 cut(s) 44, 660
RsaNI GTAC 2 cut(s) 43, 659
RseI CAYNNNNRTG 1 cut(s) 427
SacI GAGCTC 1 cut(s) 20
SatI GCNGC 3 cut(s) 6, 630, 713
Sau3AI GATC 6 cut(s) 280, 498, 663, 735, 739, 749
Sau96I GGNCC 2 cut(s) 175, 190
SchI GAGTC 1 cut(s) 155
ScrFI CCNGG 2 cut(s) 167, 642
SduI GDGCHC 3 cut(s) 20, 259, 641
SetI ASST 8 cut(s) 20, 153, 180, 192, 251, 393, 465, 733
SfaNI GCATC 2 cut(s) 306, 626
SinI GGWCC 2 cut(s) 175, 190
SmiMI CAYNNNNRTG 1 cut(s) 427
Sse9I AATT 5 cut(s) 262, 433, 611, 697, 754
SsiI CCGC 3 cut(s) 471, 508, 710
SspMI CTAG 1 cut(s) 81
SstI GAGCTC 1 cut(s) 20
StyD4I CCNGG 2 cut(s) 165, 640
TaaI ACNGT 3 cut(s) 307, 599, 721
TaqI TCGA 6 cut(s) 180, 301, 350, 477, 533, 738
TasI AATT 5 cut(s) 262, 433, 611, 697, 754
TatI WGTACW 1 cut(s) 658
TfiI GAWTC 1 cut(s) 77
TscAI CASTG 2 cut(s) 259, 604
TseFI GTSAC 1 cut(s) 567
TseI GCWGC 3 cut(s) 5, 629, 712
Tsp45I GTSAC 1 cut(s) 567
TspDTI ATGAA 2 cut(s) 390, 399
TspRI CASTG 2 cut(s) 259, 604
Van91I CCANNNNNTGG 2 cut(s) 172, 201
VpaK11BI GGWCC 2 cut(s) 175, 190
XapI RAATTY 1 cut(s) 262
XspI CTAG 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.