pycom05g20660

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0013488
Physical Location & Seq
Reverse (-)
23436465 .. 23437886
1422 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g20660.2

Sequence Viewer

Length: 750 bp
ATGGCTGCTGAGGTGAGCTCTCTGTTTCGGGTTCTAAGTGGGTACAGTGACGATCAGCATCTGAGCGCTGGGAGCGGTTCTAGTGGTGAGAAATCAACGGCTCTGATTACAAGAGACTTGCTTGGTGGTAGTGGGTCGTCTCCTTCCACGCTTCCCAACGACTCCCAGGAACTGGACCTCGACTTACAGGTCCCCAATGGCTGGGAAAAGCGCTTGGACTTGAAGTCAGGGAAAGTGTATCTTCAAAGGTGCAGTGCCCAAAATTCACATTCAATCTCATATCAAAACGACCAAACCAATCCAACAGTTCCAAAGATGCAAGATTTGAACTTCCCTCCATCGCCGTCGTCGAAAATCCCACTAAATCTCTTTGATGATACCAGCACTAACCTTGATTTGAAACTGGTTTCATCATCACCACCACCCTCATGCAGTAACCAGGGCCTATGCACTCTGGACAAGGTAAAATCCGCCCTCGAACGGGCAGAGAAGGAACCGATCAAGAGGCGATCTTCGTCCATGTTGAAGTCATCTTCGCCAACTTACTCGTCGTCATCGTCATCCATCAAAGAAACTCAGGAGGAGGACAGTGAGGACAAGCAATTTGCATCGCCATTTGCAGCAGGGTGTCCTGGTTGCCTATCTTATGTGTTGATAATGAGAAATAACCCTAAATGTCCTAGGTGCAATTCGGTTGTTCCGCTGCCTACGGTGAAGAAACCTCGGATCGATCTCAACAGATCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

250

Amino Acids

26.93

Weight (kDa)

7.52

Isoelectric Point (pI)

59.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013488)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79160
fragaria_vesca FvH4_3g15360
malus_domestica MD05G1223700.v1.1 MD10G1205200.v1.1
prunus_persica Prupe.4G136500_v2.0.a1
pyrus_communis pycom05g20660 pycom10g17570
rosa_chinensis RchiOBHm_Chr5g0025801
rosa_laevigata RLG00000032876
rosa_multiflora Rmu_sc0005610.1_g000005
rosa_roxburghii Rroxscaffold_1G00053400 Rroxscaffold_1G00054340
rosa_rugosa Rorug05G0088600
rosa_samantha Rh5AG180700 Rh5BG179300 Rh5CG197100 Rh5DG179000
rosa_wichuraiana Rw5G016390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 188
AccB7I CCANNNNNTGG 2 cut(s) 172, 201
AccBSI CCGCTC 1 cut(s) 75
AciI CCGC 3 cut(s) 75, 471, 701
AclWI GGATC 1 cut(s) 734
AcsI RAATTY 1 cut(s) 262
AfaI GTAC 1 cut(s) 44
AfeI AGCGCT 2 cut(s) 67, 212
AfiI CCNNNNNNNGG 4 cut(s) 172, 201, 480, 481
AgsI TTSAA 6 cut(s) 223, 245, 273, 328, 400, 526
AhdI GACNNNNNGTC 1 cut(s) 223
AjnI CCWGG 3 cut(s) 165, 438, 631
AluBI AGCT 1 cut(s) 18
AluI AGCT 1 cut(s) 18
Alw21I GWGCWC 1 cut(s) 20
Alw26I GTCTC 2 cut(s) 108, 144
AlwI GGATC 1 cut(s) 734
AlwNI CAGNNNCTG 2 cut(s) 61, 172
Aor51HI AGCGCT 2 cut(s) 67, 212
AoxI GGCC 1 cut(s) 442
ApeKI GCWGC 3 cut(s) 5, 620, 703
ApoI RAATTY 1 cut(s) 262
ArsI GACNNNNNNTTYG 2 cut(s) 587, 619
AspA2I CCTAGG 1 cut(s) 680
AspLEI GCGC 2 cut(s) 68, 213
AspS9I GGNCC 3 cut(s) 175, 190, 442
AsuHPI GGTGA 4 cut(s) 25, 98, 408, 724
AvaII GGWCC 2 cut(s) 175, 190
AvrII CCTAGG 1 cut(s) 680
BaeGI GKGCMC 1 cut(s) 259
BanII GRGCYC 1 cut(s) 20
Bbv12I GWGCWC 1 cut(s) 20
BbvCI CCTCAGC 1 cut(s) 9
BbvI GCAGC 2 cut(s) 632, 690
BccI CCATC 2 cut(s) 346, 572
BceAI ACGGC 2 cut(s) 114, 328
BciT130I CCWGG 3 cut(s) 167, 440, 633
BcoDI GTCTC 2 cut(s) 108, 144
BfaI CTAG 2 cut(s) 81, 681
BfoI RGCGCY 2 cut(s) 69, 214
BisI GCNGC 3 cut(s) 6, 621, 704
BlnI CCTAGG 1 cut(s) 680
BlsI GCNGC 3 cut(s) 7, 622, 705
Bme1390I CCNGG 3 cut(s) 167, 440, 633
Bme18I GGWCC 2 cut(s) 175, 190
BmeRI GACNNNNNGTC 1 cut(s) 223
BmgT120I GGNCC 3 cut(s) 175, 190, 442
BmiI GGNNCC 2 cut(s) 192, 495
BmrFI CCNGG 3 cut(s) 167, 440, 633
BmsI GCATC 3 cut(s) 67, 306, 617
BplI GAGNNNNNCTC 1 cut(s) 34
Bpu10I CCTNAGC 1 cut(s) 9
Bsa29I ATCGAT 1 cut(s) 729
BsaBI GATNNNNATC 1 cut(s) 57
BsaJI CCNNGG 4 cut(s) 165, 439, 680, 722
Bsc4I CCNNNNNNNGG 4 cut(s) 172, 201, 480, 481
Bse1I ACTGG 2 cut(s) 177, 408
Bse8I GATNNNNATC 1 cut(s) 57
BseBI CCWGG 3 cut(s) 167, 440, 633
BseCI ATCGAT 1 cut(s) 729
BseDI CCNNGG 4 cut(s) 165, 439, 680, 722
BseGI GGATG 1 cut(s) 560
BseJI GATNNNNATC 1 cut(s) 57
BseLI CCNNNNNNNGG 4 cut(s) 172, 201, 480, 481
BseMII CTCAG 2 cut(s) 53, 590
BseNI ACTGG 2 cut(s) 177, 408
BseRI GAGGAG 1 cut(s) 596
BseSI GKGCMC 1 cut(s) 259
BseXI GCAGC 2 cut(s) 632, 690
BseYI CCCAGC 2 cut(s) 68, 201
BsgI GTGCAG 1 cut(s) 271
BshFI GGCC 1 cut(s) 444
BshVI ATCGAT 1 cut(s) 729
BsiHKAI GWGCWC 1 cut(s) 20
BslFI GGGAC 1 cut(s) 176
BslI CCNNNNNNNGG 4 cut(s) 172, 201, 480, 481
BsmAI GTCTC 2 cut(s) 108, 144
BsmBI CGTCTC 1 cut(s) 144
BsmFI GGGAC 1 cut(s) 176
BsnI GGCC 1 cut(s) 444
Bsp1286I GDGCHC 2 cut(s) 20, 259
Bsp143I GATC 6 cut(s) 52, 498, 509, 726, 730, 740
BspACI CCGC 3 cut(s) 75, 471, 701
BspANI GGCC 1 cut(s) 444
BspCNI CTCAG 2 cut(s) 54, 589
BspDI ATCGAT 1 cut(s) 729
BspLI GGNNCC 2 cut(s) 192, 495
BspPI GGATC 1 cut(s) 734
BsrBI CCGCTC 1 cut(s) 75
BsrI ACTGG 2 cut(s) 177, 408
BssECI CCNNGG 4 cut(s) 165, 439, 680, 722
BssMI GATC 6 cut(s) 52, 498, 509, 726, 730, 740
BssT1I CCWWGG 1 cut(s) 680
Bst2UI CCWGG 3 cut(s) 167, 440, 633
Bst4CI ACNGT 4 cut(s) 47, 307, 590, 712
BstDEI CTNAG 4 cut(s) 9, 35, 62, 576
BstF5I GGATG 1 cut(s) 560
BstH2I RGCGCY 2 cut(s) 69, 214
BstHHI GCGC 2 cut(s) 68, 213
BstKTI GATC 6 cut(s) 55, 501, 512, 729, 733, 743
BstMAI GTCTC 2 cut(s) 108, 144
BstMBI GATC 6 cut(s) 52, 498, 509, 726, 730, 740
BstMWI GCNNNNNNNGC 1 cut(s) 72
BstNI CCWGG 3 cut(s) 167, 440, 633
BstSCI CCNGG 3 cut(s) 165, 438, 631
BstSLI GKGCMC 1 cut(s) 259
BstV1I GCAGC 2 cut(s) 632, 690
Bsu15I ATCGAT 1 cut(s) 729
BsuRI GGCC 1 cut(s) 444
BsuTUI ATCGAT 1 cut(s) 729
BtgZI GCGATG 2 cut(s) 324, 594
BtsCI GGATG 1 cut(s) 560
BtsI GCAGTG 1 cut(s) 259
BtsIMutI CAGTG 3 cut(s) 52, 259, 595
CaiI CAGNNNCTG 2 cut(s) 61, 172
CfoI GCGC 2 cut(s) 68, 213
Cfr13I GGNCC 3 cut(s) 175, 190, 442
ClaI ATCGAT 1 cut(s) 729
Csp6I GTAC 1 cut(s) 43
CviAII CATG 2 cut(s) 429, 520
CviJI RGCY 5 cut(s) 5, 18, 101, 201, 444
CviKI_1 RGCY 5 cut(s) 5, 18, 101, 201, 444
CviQI GTAC 1 cut(s) 43
DdeI CTNAG 4 cut(s) 9, 35, 62, 576
DpnI GATC 6 cut(s) 54, 500, 511, 728, 732, 742
DpnII GATC 6 cut(s) 52, 498, 509, 726, 730, 740
DrdI GACNNNNNNGTC 1 cut(s) 188
DriI GACNNNNNGTC 1 cut(s) 223
DseDI GACNNNNNNGTC 1 cut(s) 188
Eam1105I GACNNNNNGTC 1 cut(s) 223
EciI GGCGGA 1 cut(s) 460
Ecl136II GAGCTC 1 cut(s) 18
Eco130I CCWWGG 1 cut(s) 680
Eco24I GRGCYC 1 cut(s) 20
Eco47I GGWCC 2 cut(s) 175, 190
Eco47III AGCGCT 2 cut(s) 67, 212
Eco53kI GAGCTC 1 cut(s) 18
EcoICRI GAGCTC 1 cut(s) 18
EcoO109I RGGNCCY 2 cut(s) 190, 442
EcoRII CCWGG 3 cut(s) 165, 438, 631
EcoT14I CCWWGG 1 cut(s) 680
EcoT38I GRGCYC 1 cut(s) 20
ErhI CCWWGG 1 cut(s) 680
Esp3I CGTCTC 1 cut(s) 144
FaeI CATG 2 cut(s) 432, 523
FaiI YATR 5 cut(s) 280, 430, 448, 521, 648
FalI AAGNNNNNCTT 2 cut(s) 225, 257
FaqI GGGAC 1 cut(s) 176
FatI CATG 2 cut(s) 428, 519
Fnu4HI GCNGC 3 cut(s) 6, 621, 704
FokI GGATG 1 cut(s) 547
FriOI GRGCYC 1 cut(s) 20
Fsp4HI GCNGC 3 cut(s) 6, 621, 704
FspBI CTAG 2 cut(s) 81, 681
GlaI GCGC 2 cut(s) 67, 212
GluI GCNGC 3 cut(s) 6, 621, 704
GsaI CCCAGC 2 cut(s) 72, 205
HaeII RGCGCY 2 cut(s) 69, 214
HaeIII GGCC 1 cut(s) 444
HhaI GCGC 2 cut(s) 68, 213
Hin1II CATG 2 cut(s) 432, 523
Hin6I GCGC 2 cut(s) 66, 211
HinP1I GCGC 2 cut(s) 66, 211
HinfI GANTC 1 cut(s) 161
HphI GGTGA 4 cut(s) 25, 98, 408, 724
Hpy188I TCNGA 3 cut(s) 63, 105, 726
Hpy188III TCNNGA 3 cut(s) 455, 502, 578
Hpy99I CGWCG 3 cut(s) 349, 352, 553
HpyAV CCTTC 2 cut(s) 153, 484
HpyCH4III ACNGT 4 cut(s) 47, 307, 590, 712
HpyCH4V TGCA 7 cut(s) 252, 319, 432, 450, 608, 620, 687
HpyF10VI GCNNNNNNNGC 1 cut(s) 72
HpyF3I CTNAG 4 cut(s) 9, 35, 62, 576
Hsp92II CATG 2 cut(s) 432, 523
HspAI GCGC 2 cut(s) 66, 211
Kzo9I GATC 6 cut(s) 52, 498, 509, 726, 730, 740
LmnI GCTCC 1 cut(s) 72
Lsp1109I GCAGC 2 cut(s) 632, 690
LweI GCATC 3 cut(s) 67, 306, 617
MaeI CTAG 2 cut(s) 81, 681
MaeIII GTNAC 2 cut(s) 47, 434
MalI GATC 6 cut(s) 54, 500, 511, 728, 732, 742
MbiI CCGCTC 1 cut(s) 75
MboI GATC 6 cut(s) 52, 498, 509, 726, 730, 740
MboII GAAGA 4 cut(s) 233, 504, 525, 727
MhlI GDGCHC 2 cut(s) 20, 259
MluCI AATT 4 cut(s) 262, 602, 688, 745
MlyI GAGTC 1 cut(s) 155
MmeI TCCRAC 1 cut(s) 326
MslI CAYNNNNRTG 1 cut(s) 427
MspA1I CMGCKG 1 cut(s) 703
MspR9I CCNGG 3 cut(s) 167, 440, 633
MvaI CCWGG 3 cut(s) 167, 440, 633
MwoI GCNNNNNNNGC 1 cut(s) 72
NdeII GATC 6 cut(s) 52, 498, 509, 726, 730, 740
NlaIII CATG 2 cut(s) 432, 523
NlaIV GGNNCC 2 cut(s) 192, 495
NmuCI GTSAC 1 cut(s) 47
PcsI WCGNNNNNNNCGW 2 cut(s) 347, 554
PflMI CCANNNNNTGG 2 cut(s) 172, 201
PkrI GCNGC 3 cut(s) 7, 622, 705
PleI GAGTC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 155
PpuMI RGGWCCY 1 cut(s) 190
Psp124BI GAGCTC 1 cut(s) 20
Psp5II RGGWCCY 1 cut(s) 190
Psp6I CCWGG 3 cut(s) 165, 438, 631
PspFI CCCAGC 2 cut(s) 68, 201
PspGI CCWGG 3 cut(s) 165, 438, 631
PspN4I GGNNCC 2 cut(s) 192, 495
PspPI GGNCC 3 cut(s) 175, 190, 442
PspPPI RGGWCCY 1 cut(s) 190
PstNI CAGNNNCTG 2 cut(s) 61, 172
RsaI GTAC 1 cut(s) 44
RsaNI GTAC 1 cut(s) 43
RseI CAYNNNNRTG 1 cut(s) 427
SacI GAGCTC 1 cut(s) 20
SatI GCNGC 3 cut(s) 6, 621, 704
Sau3AI GATC 6 cut(s) 52, 498, 509, 726, 730, 740
Sau96I GGNCC 3 cut(s) 175, 190, 442
SchI GAGTC 1 cut(s) 155
ScrFI CCNGG 3 cut(s) 167, 440, 633
SduI GDGCHC 2 cut(s) 20, 259
SetI ASST 9 cut(s) 15, 20, 180, 192, 251, 393, 465, 686, 724
SfaNI GCATC 3 cut(s) 67, 306, 617
SinI GGWCC 2 cut(s) 175, 190
SmiMI CAYNNNNRTG 1 cut(s) 427
Sse9I AATT 4 cut(s) 262, 602, 688, 745
SsiI CCGC 3 cut(s) 75, 471, 701
SspMI CTAG 2 cut(s) 81, 681
SstI GAGCTC 1 cut(s) 20
StyD4I CCNGG 3 cut(s) 165, 438, 631
StyI CCWWGG 1 cut(s) 680
TaaI ACNGT 4 cut(s) 47, 307, 590, 712
TaqI TCGA 4 cut(s) 180, 350, 477, 729
TasI AATT 4 cut(s) 262, 602, 688, 745
TscAI CASTG 3 cut(s) 52, 259, 595
TseFI GTSAC 1 cut(s) 47
TseI GCWGC 3 cut(s) 5, 620, 703
Tsp45I GTSAC 1 cut(s) 47
TspDTI ATGAA 1 cut(s) 399
TspRI CASTG 3 cut(s) 52, 259, 595
Van91I CCANNNNNTGG 2 cut(s) 172, 201
VpaK11BI GGWCC 2 cut(s) 175, 190
XapI RAATTY 1 cut(s) 262
XmaJI CCTAGG 1 cut(s) 680
XspI CTAG 2 cut(s) 81, 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.