MD10G1238800.v1.1

Photosystem I reaction center subunit III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0015377
Physical Location & Seq
Reverse (-)
33511014 .. 33511703
690 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1238800.v1.1.491

Sequence Viewer

Length: 690 bp
ATGTCTCTCACAATTCCCACAAACCTTTCAAAACCCATGTTTCAGCCAAAACTTAGCTCCCCATTGGCCGCAAAGCTGCCAAGAACCGTGGTCTTCTGCTCTGCCTCCCCAAACCCTAACCCTACCCCAGAGAAAATATCGACTGCATCGCCCATGCAGGCTTTCTCCACTGCACTCGCACTCTCCTCCATCCTCATTGGCTCGGCCCCCATGCCTGCAGTTGCTGACATCTCGGGACTCACCCCATGCAAAGACTCCAAGCAGTTCGCCAAGCGTGAGAAGCAGCAGATCAAAAAACTCGAGTCCTCACTGAAGCTCTATGCACCCGACAGTGCACCCGCCCTAGCCATCAAGGCCACCATAGAGAAGACTCAGAGGAGGTTTTCCAACTATGGCAAGCAGGGTTTGCTTTGTGGGTCGGATGGGCTCCCCCATTTGATTGTCAGCGGTGACCAGAGGCACTGGGGTGAGTTTATCACGCCTGGTATTTTGTTCCTGTACATTGCTGGGTGGATCGGGTGGGTTGGGAGGAGCTACTTGATTGCCATTAGGGATGATAAGAAGCCGACACAGAAGGAGATCATCATTGATGTGCCTTTGGCTACTAGCTTGGTTTTCAGAGGCTTCAGCTGGCCTGTGGCTGCTTACAGAGAGTTAGTGAATGGTGAACTTGTTGCCAAGGACGTTTGA

Protein Analysis

230

Amino Acids

24.88

Weight (kDa)

9.6

Isoelectric Point (pI)

37.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSI_PsaF PF02507 66 - 228 1.7e-80 Photosystem I reaction centre subunit III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015377)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31330
fragaria_vesca FvH4_3g11800
malus_domestica MD05G1259200.v1.1 MD10G1238800.v1.1
prunus_persica Prupe.4G105600_v2.0.a1
pyrus_communis pycom10g19980
rosa_chinensis RchiOBHm_Chr5g0019221
rosa_laevigata RLG00000032429
rosa_multiflora Rmu_sc0002561.1_g000004
rosa_roxburghii Rroxscaffold_1G00058770
rosa_rugosa Rorug05G0048400
rosa_samantha Rh5AG139800 Rh5BG138800 Rh5CG150200 Rh5DG139400
rosa_wichuraiana Rw5G012430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 69, 339, 447
AclWI GGATC 1 cut(s) 521
AcoI YGGCCR 1 cut(s) 66
AcuI CTGAAG 2 cut(s) 332, 610
AfaI GTAC 1 cut(s) 500
AgsI TTSAA 1 cut(s) 30
AjnI CCWGG 1 cut(s) 481
AleI CACNNNNGTG 1 cut(s) 465
AluBI AGCT 6 cut(s) 57, 76, 316, 534, 609, 630
AluI AGCT 6 cut(s) 57, 76, 316, 534, 609, 630
Alw21I GWGCWC 1 cut(s) 337
Alw26I GTCTC 1 cut(s) 9
Alw44I GTGCAC 1 cut(s) 333
AlwI GGATC 1 cut(s) 521
AlwNI CAGNNNCTG 1 cut(s) 224
Ama87I CYCGRG 2 cut(s) 232, 299
AoxI GGCC 4 cut(s) 66, 204, 354, 632
ApaLI GTGCAC 1 cut(s) 333
ApeKI GCWGC 3 cut(s) 76, 283, 641
AspS9I GGNCC 1 cut(s) 205
AsuHPI GGTGA 4 cut(s) 232, 461, 479, 677
AvaI CYCGRG 2 cut(s) 232, 299
BaeGI GKGCMC 1 cut(s) 337
BanII GRGCYC 1 cut(s) 429
BbsI GAAGAC 2 cut(s) 85, 374
Bbv12I GWGCWC 1 cut(s) 337
BbvI GCAGC 3 cut(s) 63, 295, 628
BccI CCATC 3 cut(s) 197, 356, 416
BciT130I CCWGG 1 cut(s) 483
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 2 cut(s) 344, 606
BfmI CTRYAG 1 cut(s) 216
BglI GCCNNNNNGGC 1 cut(s) 353
BisI GCNGC 4 cut(s) 69, 77, 284, 642
BlsI GCNGC 4 cut(s) 70, 78, 285, 643
Bme1390I CCNGG 1 cut(s) 483
BmeT110I CYCGRG 2 cut(s) 232, 299
BmgT120I GGNCC 1 cut(s) 205
BmiI GGNNCC 2 cut(s) 207, 428
BmrFI CCNGG 1 cut(s) 483
BmrI ACTGGG 1 cut(s) 472
BmsI GCATC 1 cut(s) 155
BmuI ACTGGG 1 cut(s) 472
BpiI GAAGAC 2 cut(s) 85, 374
BsaJI CCNNGG 2 cut(s) 87, 678
Bse1I ACTGG 1 cut(s) 467
Bse3DI GCAATG 1 cut(s) 501
BseBI CCWGG 1 cut(s) 483
BseDI CCNNGG 2 cut(s) 87, 678
BseGI GGATG 3 cut(s) 189, 427, 559
BseMI GCAATG 1 cut(s) 501
BseMII CTCAG 1 cut(s) 386
BseNI ACTGG 1 cut(s) 467
BseRI GAGGAG 3 cut(s) 175, 391, 544
BseSI GKGCMC 1 cut(s) 337
BseXI GCAGC 3 cut(s) 63, 295, 628
BseYI CCCAGC 1 cut(s) 506
BsgI GTGCAG 1 cut(s) 156
BshFI GGCC 4 cut(s) 68, 206, 356, 634
BsiHKAI GWGCWC 1 cut(s) 337
BsiHKCI CYCGRG 2 cut(s) 232, 299
BslFI GGGAC 1 cut(s) 249
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 1 cut(s) 249
BsnI GGCC 4 cut(s) 68, 206, 356, 634
BsoBI CYCGRG 2 cut(s) 232, 299
Bsp1286I GDGCHC 2 cut(s) 337, 429
Bsp1407I TGTACA 1 cut(s) 498
Bsp143I GATC 3 cut(s) 288, 513, 579
BspACI CCGC 3 cut(s) 69, 339, 447
BspANI GGCC 4 cut(s) 68, 206, 356, 634
BspCNI CTCAG 1 cut(s) 385
BspLI GGNNCC 2 cut(s) 207, 428
BspMAI CTGCAG 1 cut(s) 220
BspPI GGATC 1 cut(s) 521
BsrDI GCAATG 1 cut(s) 501
BsrGI TGTACA 1 cut(s) 498
BsrI ACTGG 1 cut(s) 467
BssECI CCNNGG 2 cut(s) 87, 678
BssMI GATC 3 cut(s) 288, 513, 579
BssT1I CCWWGG 1 cut(s) 678
Bst2UI CCWGG 1 cut(s) 483
Bst4CI ACNGT 2 cut(s) 88, 332
BstAPI GCANNNNNTGC 1 cut(s) 406
BstAUI TGTACA 1 cut(s) 498
BstC8I GCNNGC 4 cut(s) 159, 216, 398, 632
BstDEI CTNAG 2 cut(s) 53, 372
BstDSI CCRYGG 1 cut(s) 87
BstEII GGTNACC 1 cut(s) 449
BstF5I GGATG 3 cut(s) 189, 427, 559
BstKTI GATC 3 cut(s) 291, 516, 582
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 3 cut(s) 288, 513, 579
BstMWI GCNNNNNNNGC 3 cut(s) 280, 353, 406
BstNI CCWGG 1 cut(s) 483
BstPI GGTNACC 1 cut(s) 449
BstSCI CCNGG 1 cut(s) 481
BstSFI CTRYAG 1 cut(s) 216
BstSLI GKGCMC 1 cut(s) 337
BstV1I GCAGC 3 cut(s) 63, 295, 628
BstV2I GAAGAC 2 cut(s) 85, 374
BsuRI GGCC 4 cut(s) 68, 206, 356, 634
BtgI CCRYGG 1 cut(s) 87
BtgZI GCGATG 1 cut(s) 132
BtsCI GGATG 3 cut(s) 189, 427, 559
BtsI GCAGTG 1 cut(s) 168
BtsIMutI CAGTG 4 cut(s) 168, 308, 337, 460
Cac8I GCNNGC 4 cut(s) 159, 216, 398, 632
CaiI CAGNNNCTG 1 cut(s) 224
Cfr13I GGNCC 1 cut(s) 205
Csp6I GTAC 1 cut(s) 499
CspCI CAANNNNNGTGG 2 cut(s) 69, 104
CviAII CATG 4 cut(s) 37, 154, 211, 246
CviQI GTAC 1 cut(s) 499
DdeI CTNAG 2 cut(s) 53, 372
DpnI GATC 3 cut(s) 290, 515, 581
DpnII GATC 3 cut(s) 288, 513, 579
EaeI YGGCCR 1 cut(s) 66
Eco130I CCWWGG 1 cut(s) 678
Eco24I GRGCYC 1 cut(s) 429
Eco57I CTGAAG 2 cut(s) 332, 610
Eco88I CYCGRG 2 cut(s) 232, 299
Eco91I GGTNACC 1 cut(s) 449
EcoO65I GGTNACC 1 cut(s) 449
EcoRII CCWGG 1 cut(s) 481
EcoT14I CCWWGG 1 cut(s) 678
EcoT38I GRGCYC 1 cut(s) 429
ErhI CCWWGG 1 cut(s) 678
FaeI CATG 4 cut(s) 40, 157, 214, 249
FaiI YATR 7 cut(s) 38, 155, 212, 247, 321, 362, 393
FaqI GGGAC 1 cut(s) 249
FatI CATG 4 cut(s) 36, 153, 210, 245
FauI CCCGC 1 cut(s) 346
Fnu4HI GCNGC 4 cut(s) 69, 77, 284, 642
FokI GGATG 3 cut(s) 176, 434, 566
FriOI GRGCYC 1 cut(s) 429
Fsp4HI GCNGC 4 cut(s) 69, 77, 284, 642
FspBI CTAG 2 cut(s) 344, 606
GluI GCNGC 4 cut(s) 69, 77, 284, 642
GsaI CCCAGC 1 cut(s) 510
HaeIII GGCC 4 cut(s) 68, 206, 356, 634
Hin1II CATG 4 cut(s) 40, 157, 214, 249
HinfI GANTC 4 cut(s) 237, 254, 302, 370
HphI GGTGA 4 cut(s) 232, 461, 479, 677
Hpy166II GTNNAC 2 cut(s) 335, 668
Hpy188I TCNGA 3 cut(s) 375, 421, 620
Hpy188III TCNNGA 1 cut(s) 234
Hpy8I GTNNAC 2 cut(s) 335, 668
HpyAV CCTTC 1 cut(s) 568
HpyCH4III ACNGT 2 cut(s) 88, 332
HpyCH4IV ACGT 1 cut(s) 684
HpyCH4V TGCA 7 cut(s) 146, 157, 173, 218, 249, 323, 335
HpyF10VI GCNNNNNNNGC 3 cut(s) 280, 353, 406
HpyF3I CTNAG 2 cut(s) 53, 372
HpySE526I ACGT 1 cut(s) 684
Hsp92II CATG 4 cut(s) 40, 157, 214, 249
Kzo9I GATC 3 cut(s) 288, 513, 579
LmnI GCTCC 3 cut(s) 62, 432, 531
Lsp1109I GCAGC 3 cut(s) 63, 295, 628
LweI GCATC 1 cut(s) 155
MaeI CTAG 2 cut(s) 344, 606
MaeII ACGT 1 cut(s) 684
MaeIII GTNAC 1 cut(s) 449
MalI GATC 3 cut(s) 290, 515, 581
MboI GATC 3 cut(s) 288, 513, 579
MboII GAAGA 2 cut(s) 85, 379
MhlI GDGCHC 2 cut(s) 337, 429
MluCI AATT 1 cut(s) 12
MlyI GAGTC 4 cut(s) 231, 248, 311, 364
MmeI TCCRAC 2 cut(s) 399, 411
MnlI CCTC 9 cut(s) 115, 196, 203, 316, 369, 372, 450, 522, 614
MslI CAYNNNNRTG 2 cut(s) 465, 590
MspA1I CMGCKG 2 cut(s) 447, 630
MspR9I CCNGG 1 cut(s) 483
MvaI CCWGG 1 cut(s) 483
MwoI GCNNNNNNNGC 3 cut(s) 280, 353, 406
NdeII GATC 3 cut(s) 288, 513, 579
NlaIII CATG 4 cut(s) 40, 157, 214, 249
NlaIV GGNNCC 2 cut(s) 207, 428
NmeAIII GCCGAG 1 cut(s) 182
NmuCI GTSAC 1 cut(s) 449
OliI CACNNNNGTG 1 cut(s) 465
PaeR7I CTCGAG 1 cut(s) 299
PkrI GCNGC 4 cut(s) 70, 78, 285, 643
PleI GAGTC 4 cut(s) 231, 248, 310, 364
PpsI GAGTC 4 cut(s) 231, 248, 310, 364
Psp6I CCWGG 1 cut(s) 481
PspEI GGTNACC 1 cut(s) 449
PspFI CCCAGC 1 cut(s) 506
PspGI CCWGG 1 cut(s) 481
PspN4I GGNNCC 2 cut(s) 207, 428
PspPI GGNCC 1 cut(s) 205
PspXI VCTCGAGB 1 cut(s) 299
PstI CTGCAG 1 cut(s) 220
PstNI CAGNNNCTG 1 cut(s) 224
PvuII CAGCTG 1 cut(s) 630
RsaI GTAC 1 cut(s) 500
RsaNI GTAC 1 cut(s) 499
RseI CAYNNNNRTG 2 cut(s) 465, 590
SatI GCNGC 4 cut(s) 69, 77, 284, 642
Sau3AI GATC 3 cut(s) 288, 513, 579
Sau96I GGNCC 1 cut(s) 205
SchI GAGTC 4 cut(s) 231, 248, 311, 364
ScrFI CCNGG 1 cut(s) 483
SduI GDGCHC 2 cut(s) 337, 429
SetI ASST 9 cut(s) 27, 59, 78, 318, 383, 536, 611, 632, 687
SfaNI GCATC 1 cut(s) 155
SfcI CTRYAG 1 cut(s) 216
Sfr274I CTCGAG 1 cut(s) 299
SlaI CTCGAG 1 cut(s) 299
SmiMI CAYNNNNRTG 2 cut(s) 465, 590
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
Sse9I AATT 1 cut(s) 12
SsiI CCGC 3 cut(s) 69, 339, 447
SspMI CTAG 2 cut(s) 344, 606
StyD4I CCNGG 1 cut(s) 481
StyI CCWWGG 1 cut(s) 678
TaaI ACNGT 2 cut(s) 88, 332
TaiI ACGT 1 cut(s) 687
TaqI TCGA 2 cut(s) 140, 300
TasI AATT 1 cut(s) 12
TatI WGTACW 1 cut(s) 498
TauI GCSGC 1 cut(s) 71
TscAI CASTG 4 cut(s) 175, 315, 337, 467
TseFI GTSAC 1 cut(s) 449
TseI GCWGC 3 cut(s) 76, 283, 641
Tsp45I GTSAC 1 cut(s) 449
TspRI CASTG 4 cut(s) 175, 315, 337, 467
VneI GTGCAC 1 cut(s) 333
XhoI CTCGAG 1 cut(s) 299
XspI CTAG 2 cut(s) 344, 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.