Rh5BG138800

Photosystem I reaction center subunit III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0015377
Physical Location & Seq
Forward (+)
14283732 .. 14286261
2530 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG138800.1

Sequence Viewer

Length: 678 bp
ATGTCTCTCACTATTCCCACAAACCTCTCAAAATCCATGTTGAAACCTAAGCTCAGCTCTCCCCTGGCCCTAAAGCCAAGGACCATGGTCACTGTGTGCTCAGCCACCCCAAACCCAGAGACAATATCCACTGCTTCAGCAATGAAGACTTTCTCTGCTGCACTAGCACTCTCCTCCATCATCTTGGGCGCAGCTCCCATCCCAGCCATGGCTGACATCTCAGGCCTAACCCCATGCAAAGACTCCAAGCAGTTCGCCAAGCGCGAGAAGCAGTCGATCAAGAAGCTTGAGTCCTCATTGAAGCTGTACGCACCGGACAGCGCACCTGCCTTGGCCATCAATGCAACAATAGAGAAGACCAAGAGAAGGTTTGACAACTACGGCAAGCAGGGTTTGCTCTGTGGGTCTGATGGGCTTCCCCATTTGATTGTGAGCGGTGACCAGAGGCACTGGGGTGAGTTCATCACTCCCGGGATTCTGTTCCTTTACATTGCTGGGTGGATCGGGTGGGTTGGAAGGAGCTACTTGATTGCCATTAGGGATGAAAAGAAGCCAACACAGAAGGAGATCATCATTGATGTGCCTTTGGCTACTGGCTTGGTTTTCAGAGGCTTCAGTTGGCCCATTGCTGCTTACAGAGAGTTGTTGAATGGTGAACTCATTGCCAAGGACGTGTAA

Protein Analysis

225

Amino Acids

24.4

Weight (kDa)

9.58

Isoelectric Point (pI)

34.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSI_PsaF PF02507 62 - 224 1.3e-80 Photosystem I reaction centre subunit III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015377)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31330
fragaria_vesca FvH4_3g11800
malus_domestica MD05G1259200.v1.1 MD10G1238800.v1.1
prunus_persica Prupe.4G105600_v2.0.a1
pyrus_communis pycom10g19980
rosa_chinensis RchiOBHm_Chr5g0019221
rosa_laevigata RLG00000032429
rosa_multiflora Rmu_sc0002561.1_g000004
rosa_roxburghii Rroxscaffold_1G00058770
rosa_rugosa Rorug05G0048400
rosa_samantha Rh5AG139800 Rh5BG138800 Rh5CG150200 Rh5DG139400
rosa_wichuraiana Rw5G012430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 334
Acc36I ACCTGC 1 cut(s) 334
AccBSI CCGCTC 1 cut(s) 435
AccII CGCG 1 cut(s) 264
AciI CCGC 1 cut(s) 435
AclWI GGATC 1 cut(s) 509
AcoI YGGCCR 1 cut(s) 333
AcuI CTGAAG 2 cut(s) 120, 598
AdeI CACNNNGTG 1 cut(s) 96
AfaI GTAC 1 cut(s) 308
AfiI CCNNNNNNNGG 2 cut(s) 208, 366
AflIII ACRYGT 1 cut(s) 672
AgsI TTSAA 3 cut(s) 43, 301, 649
AjiI CACGTC 1 cut(s) 673
AjnI CCWGG 1 cut(s) 63
AleI CACNNNNGTG 1 cut(s) 453
AluBI AGCT 6 cut(s) 52, 57, 194, 286, 304, 522
AluI AGCT 6 cut(s) 52, 57, 194, 286, 304, 522
Alw21I GWGCWC 1 cut(s) 101
Alw26I GTCTC 2 cut(s) 9, 113
AlwI GGATC 1 cut(s) 509
Ama87I CYCGRG 1 cut(s) 470
AoxI GGCC 4 cut(s) 66, 223, 333, 620
ApeKI GCWGC 3 cut(s) 158, 191, 629
Asp700I GAANNNNTTC 1 cut(s) 149
AspLEI GCGC 3 cut(s) 191, 264, 323
AspS9I GGNCC 3 cut(s) 67, 81, 621
AsuC2I CCSGG 2 cut(s) 471, 472
AsuHPI GGTGA 3 cut(s) 449, 467, 665
AvaI CYCGRG 1 cut(s) 470
AvaII GGWCC 1 cut(s) 81
BalI TGGCCA 1 cut(s) 335
BbsI GAAGAC 2 cut(s) 152, 362
Bbv12I GWGCWC 1 cut(s) 101
BbvI GCAGC 3 cut(s) 145, 203, 616
BccI CCATC 4 cut(s) 185, 206, 344, 404
BceAI ACGGC 1 cut(s) 397
BciT130I CCWGG 1 cut(s) 65
BcnI CCSGG 2 cut(s) 471, 472
BcoDI GTCTC 2 cut(s) 9, 113
BfaI CTAG 1 cut(s) 164
BfuAI ACCTGC 1 cut(s) 334
BisI GCNGC 3 cut(s) 159, 192, 630
BlpI GCTNAGC 2 cut(s) 53, 100
BlsI GCNGC 3 cut(s) 160, 193, 631
Bme1390I CCNGG 3 cut(s) 65, 471, 472
Bme18I GGWCC 1 cut(s) 81
BmeT110I CYCGRG 1 cut(s) 470
BmgBI CACGTC 1 cut(s) 673
BmgT120I GGNCC 3 cut(s) 67, 81, 621
BmrFI CCNGG 3 cut(s) 65, 471, 472
BmrI ACTGGG 1 cut(s) 460
BmuI ACTGGG 1 cut(s) 460
BoxI GACNNNNGTC 1 cut(s) 86
BpiI GAAGAC 2 cut(s) 152, 362
Bpu10I CCTNAGC 1 cut(s) 48
Bpu1102I GCTNAGC 2 cut(s) 53, 100
BpuEI CTTGAG 1 cut(s) 308
BpuMI CCSGG 2 cut(s) 471, 472
BsaJI CCNNGG 7 cut(s) 63, 77, 84, 207, 330, 470, 666
BsaWI WCCGGW 1 cut(s) 313
Bsc4I CCNNNNNNNGG 2 cut(s) 208, 366
Bse1I ACTGG 2 cut(s) 455, 598
Bse3DI GCAATG 4 cut(s) 147, 489, 624, 660
BseBI CCWGG 1 cut(s) 65
BseDI CCNNGG 7 cut(s) 63, 77, 84, 207, 330, 470, 666
BseGI GGATG 2 cut(s) 198, 547
BseLI CCNNNNNNNGG 2 cut(s) 208, 366
BseMI GCAATG 4 cut(s) 147, 489, 624, 660
BseMII CTCAG 3 cut(s) 67, 114, 234
BseNI ACTGG 2 cut(s) 455, 598
BseRI GAGGAG 1 cut(s) 163
BseXI GCAGC 3 cut(s) 145, 203, 616
BseYI CCCAGC 2 cut(s) 202, 494
BsgI GTGCAG 1 cut(s) 144
Bsh1236I CGCG 1 cut(s) 264
BshFI GGCC 4 cut(s) 68, 225, 335, 622
BsiHKAI GWGCWC 1 cut(s) 101
BsiHKCI CYCGRG 1 cut(s) 470
BsiSI CCGG 2 cut(s) 314, 471
BslI CCNNNNNNNGG 2 cut(s) 208, 366
BsmAI GTCTC 2 cut(s) 9, 113
BsnI GGCC 4 cut(s) 68, 225, 335, 622
BsoBI CYCGRG 1 cut(s) 470
Bsp1286I GDGCHC 1 cut(s) 101
Bsp143I GATC 3 cut(s) 276, 501, 567
Bsp1720I GCTNAGC 2 cut(s) 53, 100
Bsp19I CCATGG 2 cut(s) 84, 207
BspACI CCGC 1 cut(s) 435
BspANI GGCC 4 cut(s) 68, 225, 335, 622
BspCNI CTCAG 3 cut(s) 66, 113, 233
BspFNI CGCG 1 cut(s) 264
BspMI ACCTGC 1 cut(s) 334
BspPI GGATC 1 cut(s) 509
BsrBI CCGCTC 1 cut(s) 435
BsrDI GCAATG 4 cut(s) 147, 489, 624, 660
BsrI ACTGG 2 cut(s) 455, 598
BssECI CCNNGG 7 cut(s) 63, 77, 84, 207, 330, 470, 666
BssMI GATC 3 cut(s) 276, 501, 567
BssT1I CCWWGG 5 cut(s) 77, 84, 207, 330, 666
Bst2UI CCWGG 1 cut(s) 65
Bst4CI ACNGT 1 cut(s) 94
BstAPI GCANNNNNTGC 1 cut(s) 394
BstC8I GCNNGC 1 cut(s) 386
BstDEI CTNAG 4 cut(s) 48, 53, 100, 220
BstDSI CCRYGG 2 cut(s) 84, 207
BstEII GGTNACC 1 cut(s) 437
BstF5I GGATG 2 cut(s) 198, 547
BstFNI CGCG 1 cut(s) 264
BstHHI GCGC 3 cut(s) 191, 264, 323
BstKTI GATC 3 cut(s) 279, 504, 570
BstMAI GTCTC 2 cut(s) 9, 113
BstMBI GATC 3 cut(s) 276, 501, 567
BstMWI GCNNNNNNNGC 4 cut(s) 164, 268, 341, 394
BstNI CCWGG 1 cut(s) 65
BstPAI GACNNNNGTC 1 cut(s) 86
BstPI GGTNACC 1 cut(s) 437
BstSCI CCNGG 3 cut(s) 63, 469, 470
BstUI CGCG 1 cut(s) 264
BstV1I GCAGC 3 cut(s) 145, 203, 616
BstV2I GAAGAC 2 cut(s) 152, 362
BstXI CCANNNNNNTGG 1 cut(s) 184
BsuRI GGCC 4 cut(s) 68, 225, 335, 622
BtgI CCRYGG 2 cut(s) 84, 207
BtrI CACGTC 1 cut(s) 673
BtsCI GGATG 2 cut(s) 198, 547
BtsI GCAGTG 1 cut(s) 129
BtsIMutI CAGTG 3 cut(s) 90, 129, 448
BveI ACCTGC 1 cut(s) 334
Cac8I GCNNGC 1 cut(s) 386
CfoI GCGC 3 cut(s) 191, 264, 323
Cfr13I GGNCC 3 cut(s) 67, 81, 621
Cfr9I CCCGGG 1 cut(s) 470
Csp6I GTAC 1 cut(s) 307
CviAII CATG 4 cut(s) 37, 85, 208, 234
CviQI GTAC 1 cut(s) 307
DdeI CTNAG 4 cut(s) 48, 53, 100, 220
DpnI GATC 3 cut(s) 278, 503, 569
DpnII GATC 3 cut(s) 276, 501, 567
DraIII CACNNNGTG 1 cut(s) 96
EaeI YGGCCR 1 cut(s) 333
Eco130I CCWWGG 5 cut(s) 77, 84, 207, 330, 666
Eco147I AGGCCT 1 cut(s) 225
Eco47I GGWCC 1 cut(s) 81
Eco57I CTGAAG 2 cut(s) 120, 598
Eco88I CYCGRG 1 cut(s) 470
Eco91I GGTNACC 1 cut(s) 437
EcoO65I GGTNACC 1 cut(s) 437
EcoRII CCWGG 1 cut(s) 63
EcoT14I CCWWGG 5 cut(s) 77, 84, 207, 330, 666
ErhI CCWWGG 5 cut(s) 77, 84, 207, 330, 666
FaeI CATG 4 cut(s) 40, 88, 211, 237
FaiI YATR 4 cut(s) 38, 86, 209, 235
FatI CATG 4 cut(s) 36, 84, 207, 233
Fnu4HI GCNGC 3 cut(s) 159, 192, 630
FokI GGATG 2 cut(s) 185, 554
Fsp4HI GCNGC 3 cut(s) 159, 192, 630
FspBI CTAG 1 cut(s) 164
GlaI GCGC 3 cut(s) 190, 263, 322
GluI GCNGC 3 cut(s) 159, 192, 630
GsaI CCCAGC 2 cut(s) 206, 498
HaeIII GGCC 4 cut(s) 68, 225, 335, 622
HapII CCGG 2 cut(s) 314, 471
HhaI GCGC 3 cut(s) 191, 264, 323
Hin1II CATG 4 cut(s) 40, 88, 211, 237
Hin6I GCGC 3 cut(s) 189, 262, 321
HinP1I GCGC 3 cut(s) 189, 262, 321
HindIII AAGCTT 1 cut(s) 284
HinfI GANTC 3 cut(s) 242, 290, 475
HpaII CCGG 2 cut(s) 314, 471
HphI GGTGA 3 cut(s) 449, 467, 665
Hpy166II GTNNAC 1 cut(s) 656
Hpy188I TCNGA 2 cut(s) 409, 608
Hpy188III TCNNGA 1 cut(s) 280
Hpy8I GTNNAC 1 cut(s) 656
HpyAV CCTTC 3 cut(s) 360, 510, 556
HpyCH4III ACNGT 1 cut(s) 94
HpyCH4IV ACGT 1 cut(s) 672
HpyCH4V TGCA 3 cut(s) 161, 237, 344
HpyF10VI GCNNNNNNNGC 4 cut(s) 164, 268, 341, 394
HpyF3I CTNAG 4 cut(s) 48, 53, 100, 220
HpySE526I ACGT 1 cut(s) 672
Hsp92II CATG 4 cut(s) 40, 88, 211, 237
HspAI GCGC 3 cut(s) 189, 262, 321
Kzo9I GATC 3 cut(s) 276, 501, 567
LmnI GCTCC 2 cut(s) 199, 519
Lsp1109I GCAGC 3 cut(s) 145, 203, 616
MaeI CTAG 1 cut(s) 164
MaeII ACGT 1 cut(s) 672
MaeIII GTNAC 2 cut(s) 88, 437
MalI GATC 3 cut(s) 278, 503, 569
MbiI CCGCTC 1 cut(s) 435
MboI GATC 3 cut(s) 276, 501, 567
MboII GAAGA 2 cut(s) 157, 367
MhlI GDGCHC 1 cut(s) 101
MlsI TGGCCA 1 cut(s) 335
MluNI TGGCCA 1 cut(s) 335
MlyI GAGTC 2 cut(s) 236, 299
MmeI TCCRAC 1 cut(s) 493
MnlI CCTC 5 cut(s) 35, 184, 304, 438, 602
Mox20I TGGCCA 1 cut(s) 335
MroXI GAANNNNTTC 1 cut(s) 149
MscI TGGCCA 1 cut(s) 335
MslI CAYNNNNRTG 2 cut(s) 453, 578
Msp20I TGGCCA 1 cut(s) 335
MspI CCGG 2 cut(s) 314, 471
MspR9I CCNGG 3 cut(s) 65, 471, 472
MvaI CCWGG 1 cut(s) 65
MvnI CGCG 1 cut(s) 264
MwoI GCNNNNNNNGC 4 cut(s) 164, 268, 341, 394
NciI CCSGG 2 cut(s) 471, 472
NcoI CCATGG 2 cut(s) 84, 207
NdeII GATC 3 cut(s) 276, 501, 567
NlaIII CATG 4 cut(s) 40, 88, 211, 237
NmuCI GTSAC 2 cut(s) 88, 437
OliI CACNNNNGTG 1 cut(s) 453
PaqCI CACCTGC 1 cut(s) 334
PceI AGGCCT 1 cut(s) 225
PcsI WCGNNNNNNNCGW 1 cut(s) 261
PdmI GAANNNNTTC 1 cut(s) 149
PfeI GAWTC 1 cut(s) 475
PkrI GCNGC 3 cut(s) 160, 193, 631
PleI GAGTC 2 cut(s) 236, 298
PpsI GAGTC 2 cut(s) 236, 298
PshAI GACNNNNGTC 1 cut(s) 86
Psp6I CCWGG 1 cut(s) 63
PspEI GGTNACC 1 cut(s) 437
PspFI CCCAGC 2 cut(s) 202, 494
PspGI CCWGG 1 cut(s) 63
PspPI GGNCC 3 cut(s) 67, 81, 621
RsaI GTAC 1 cut(s) 308
RsaNI GTAC 1 cut(s) 307
RseI CAYNNNNRTG 2 cut(s) 453, 578
SatI GCNGC 3 cut(s) 159, 192, 630
Sau3AI GATC 3 cut(s) 276, 501, 567
Sau96I GGNCC 3 cut(s) 67, 81, 621
SchI GAGTC 2 cut(s) 236, 299
ScrFI CCNGG 3 cut(s) 65, 471, 472
SduI GDGCHC 1 cut(s) 101
SinI GGWCC 1 cut(s) 81
SmaI CCCGGG 1 cut(s) 472
SmiMI CAYNNNNRTG 2 cut(s) 453, 578
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
SseBI AGGCCT 1 cut(s) 225
SsiI CCGC 1 cut(s) 435
SspMI CTAG 1 cut(s) 164
StuI AGGCCT 1 cut(s) 225
StyD4I CCNGG 3 cut(s) 63, 469, 470
StyI CCWWGG 5 cut(s) 77, 84, 207, 330, 666
TaaI ACNGT 1 cut(s) 94
TaiI ACGT 1 cut(s) 675
TaqI TCGA 1 cut(s) 275
TfiI GAWTC 1 cut(s) 475
TscAI CASTG 3 cut(s) 97, 136, 455
TseFI GTSAC 2 cut(s) 88, 437
TseI GCWGC 3 cut(s) 158, 191, 629
Tsp45I GTSAC 2 cut(s) 88, 437
TspDTI ATGAA 3 cut(s) 158, 451, 558
TspMI CCCGGG 1 cut(s) 470
TspRI CASTG 3 cut(s) 97, 136, 455
VpaK11BI GGWCC 1 cut(s) 81
XcmI CCANNNNNNNNNTGG 1 cut(s) 205
XmaI CCCGGG 1 cut(s) 470
XmnI GAANNNNTTC 1 cut(s) 149
XspI CTAG 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.