RLG00000032429

Photosystem I reaction center subunit III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0015377
Physical Location & Seq
Forward (+)
15088082 .. 15088941
860 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032429

Sequence Viewer

Length: 654 bp
ATGTTGAAACCTAAGCTCAGCTCTCCCTTGGCCCTAAAGCCAAGGACCATGGTCACTGTGTGCTCAGCCACCCCAAACCCAGAGAAAATATCCACTGCTTCAGCAATGAAGACTTTCTCTGCTGCACTAGCACTCTCCTCCATCATCTTGGGCGCAGCTCCCATCCCAGCCATGGCTGACATCTCAGGCCTAACCCCATGCAAAGACTCCAAGCAGTTCGCCAAGCGCGAGAAGCAGTCGATCAAGAAGCTCGAGTCCTCATTGAAGCTGTACGCACCAGACAGCGCACCTGCCTTGGCCATCAATGCAACAATAGAGAAGACCAAGAGAAGGTTTGACAACTACGGCAAGCAGGGTTTGCTCTGTGGGTCTGATGGGCTTCCCCATTTGATTGTGAGCGGTGACCAGAGGCACTGGGGTGAGTTCATCACTCCCGGGATTCTGTTCCTTTACATTGCTGGGTGGATCGGGTGGGTTGGAAGGAGCTACTTGATTGCCATTAGGGATGATAAGAAGCCAACACAGAAGGAGATCATCATTGATGTGCCTTTGGCTACTGGCTTGGTTTTCAGAGGCTTCAGTTGGCCCGTTGCTGCTTACAGAGAGTTGGTGAATGGTGAACTCGTTGCCAAGGACGTGGTGTGTAACCTATAG

Protein Analysis

218

Amino Acids

23.53

Weight (kDa)

9.51

Isoelectric Point (pI)

34.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSI_PsaF PF02507 50 - 212 8.4e-81 Photosystem I reaction centre subunit III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015377)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31330
fragaria_vesca FvH4_3g11800
malus_domestica MD05G1259200.v1.1 MD10G1238800.v1.1
prunus_persica Prupe.4G105600_v2.0.a1
pyrus_communis pycom10g19980
rosa_chinensis RchiOBHm_Chr5g0019221
rosa_laevigata RLG00000032429
rosa_multiflora Rmu_sc0002561.1_g000004
rosa_roxburghii Rroxscaffold_1G00058770
rosa_rugosa Rorug05G0048400
rosa_samantha Rh5AG139800 Rh5BG138800 Rh5CG150200 Rh5DG139400
rosa_wichuraiana Rw5G012430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 298
Acc36I ACCTGC 1 cut(s) 298
AccBSI CCGCTC 1 cut(s) 399
AccII CGCG 1 cut(s) 228
AciI CCGC 1 cut(s) 399
AclWI GGATC 1 cut(s) 473
AcoI YGGCCR 1 cut(s) 297
AcuI CTGAAG 2 cut(s) 84, 562
AdeI CACNNNGTG 1 cut(s) 60
AfaI GTAC 1 cut(s) 272
AfiI CCNNNNNNNGG 2 cut(s) 172, 330
AgsI TTSAA 2 cut(s) 7, 265
AjiI CACGTC 1 cut(s) 637
AleI CACNNNNGTG 1 cut(s) 417
AluBI AGCT 6 cut(s) 16, 21, 158, 250, 268, 486
AluI AGCT 6 cut(s) 16, 21, 158, 250, 268, 486
Alw21I GWGCWC 1 cut(s) 65
AlwI GGATC 1 cut(s) 473
Ama87I CYCGRG 2 cut(s) 251, 434
AoxI GGCC 4 cut(s) 30, 187, 297, 584
ApeKI GCWGC 3 cut(s) 122, 155, 593
Asp700I GAANNNNTTC 1 cut(s) 113
AspLEI GCGC 3 cut(s) 155, 228, 287
AspS9I GGNCC 3 cut(s) 31, 45, 585
AsuC2I CCSGG 2 cut(s) 435, 436
AsuHPI GGTGA 4 cut(s) 413, 431, 622, 629
AvaI CYCGRG 2 cut(s) 251, 434
AvaII GGWCC 1 cut(s) 45
BalI TGGCCA 1 cut(s) 299
BbsI GAAGAC 2 cut(s) 116, 326
Bbv12I GWGCWC 1 cut(s) 65
BbvI GCAGC 3 cut(s) 109, 167, 580
BccI CCATC 4 cut(s) 149, 170, 308, 368
BceAI ACGGC 1 cut(s) 361
BcnI CCSGG 2 cut(s) 435, 436
BfaI CTAG 1 cut(s) 128
BfmI CTRYAG 1 cut(s) 650
BfuAI ACCTGC 1 cut(s) 298
BisI GCNGC 3 cut(s) 123, 156, 594
BlpI GCTNAGC 2 cut(s) 17, 64
BlsI GCNGC 3 cut(s) 124, 157, 595
Bme1390I CCNGG 2 cut(s) 435, 436
Bme18I GGWCC 1 cut(s) 45
BmeT110I CYCGRG 2 cut(s) 251, 434
BmgBI CACGTC 1 cut(s) 637
BmgT120I GGNCC 3 cut(s) 31, 45, 585
BmrFI CCNGG 2 cut(s) 435, 436
BmrI ACTGGG 1 cut(s) 424
BmuI ACTGGG 1 cut(s) 424
BoxI GACNNNNGTC 1 cut(s) 50
BpiI GAAGAC 2 cut(s) 116, 326
Bpu10I CCTNAGC 1 cut(s) 12
Bpu1102I GCTNAGC 2 cut(s) 17, 64
BpuMI CCSGG 2 cut(s) 435, 436
BsaJI CCNNGG 7 cut(s) 27, 41, 48, 171, 294, 434, 630
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 330
Bse1I ACTGG 2 cut(s) 419, 562
Bse3DI GCAATG 2 cut(s) 111, 453
BseDI CCNNGG 7 cut(s) 27, 41, 48, 171, 294, 434, 630
BseGI GGATG 2 cut(s) 162, 511
BseLI CCNNNNNNNGG 2 cut(s) 172, 330
BseMI GCAATG 2 cut(s) 111, 453
BseMII CTCAG 3 cut(s) 31, 78, 198
BseNI ACTGG 2 cut(s) 419, 562
BseRI GAGGAG 1 cut(s) 127
BseXI GCAGC 3 cut(s) 109, 167, 580
BseYI CCCAGC 2 cut(s) 166, 458
BsgI GTGCAG 1 cut(s) 108
Bsh1236I CGCG 1 cut(s) 228
BshFI GGCC 4 cut(s) 32, 189, 299, 586
BsiHKAI GWGCWC 1 cut(s) 65
BsiHKCI CYCGRG 2 cut(s) 251, 434
BsiSI CCGG 1 cut(s) 435
BslI CCNNNNNNNGG 2 cut(s) 172, 330
BsnI GGCC 4 cut(s) 32, 189, 299, 586
BsoBI CYCGRG 2 cut(s) 251, 434
Bsp1286I GDGCHC 1 cut(s) 65
Bsp143I GATC 3 cut(s) 240, 465, 531
Bsp1720I GCTNAGC 2 cut(s) 17, 64
Bsp19I CCATGG 2 cut(s) 48, 171
BspACI CCGC 1 cut(s) 399
BspANI GGCC 4 cut(s) 32, 189, 299, 586
BspCNI CTCAG 3 cut(s) 30, 77, 197
BspFNI CGCG 1 cut(s) 228
BspMI ACCTGC 1 cut(s) 298
BspPI GGATC 1 cut(s) 473
BsrBI CCGCTC 1 cut(s) 399
BsrDI GCAATG 2 cut(s) 111, 453
BsrI ACTGG 2 cut(s) 419, 562
BssECI CCNNGG 7 cut(s) 27, 41, 48, 171, 294, 434, 630
BssMI GATC 3 cut(s) 240, 465, 531
BssT1I CCWWGG 6 cut(s) 27, 41, 48, 171, 294, 630
Bst4CI ACNGT 1 cut(s) 58
BstAPI GCANNNNNTGC 1 cut(s) 358
BstC8I GCNNGC 1 cut(s) 350
BstDEI CTNAG 4 cut(s) 12, 17, 64, 184
BstDSI CCRYGG 2 cut(s) 48, 171
BstEII GGTNACC 1 cut(s) 401
BstF5I GGATG 2 cut(s) 162, 511
BstFNI CGCG 1 cut(s) 228
BstHHI GCGC 3 cut(s) 155, 228, 287
BstKTI GATC 3 cut(s) 243, 468, 534
BstMBI GATC 3 cut(s) 240, 465, 531
BstMWI GCNNNNNNNGC 4 cut(s) 128, 232, 305, 358
BstPAI GACNNNNGTC 1 cut(s) 50
BstPI GGTNACC 1 cut(s) 401
BstSCI CCNGG 2 cut(s) 433, 434
BstSFI CTRYAG 1 cut(s) 650
BstUI CGCG 1 cut(s) 228
BstV1I GCAGC 3 cut(s) 109, 167, 580
BstV2I GAAGAC 2 cut(s) 116, 326
BstXI CCANNNNNNTGG 2 cut(s) 148, 637
BsuRI GGCC 4 cut(s) 32, 189, 299, 586
BtgI CCRYGG 2 cut(s) 48, 171
BtrI CACGTC 1 cut(s) 637
BtsCI GGATG 2 cut(s) 162, 511
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 3 cut(s) 54, 93, 412
BveI ACCTGC 1 cut(s) 298
Cac8I GCNNGC 1 cut(s) 350
CfoI GCGC 3 cut(s) 155, 228, 287
Cfr13I GGNCC 3 cut(s) 31, 45, 585
Cfr9I CCCGGG 1 cut(s) 434
Csp6I GTAC 1 cut(s) 271
CviAII CATG 3 cut(s) 49, 172, 198
CviQI GTAC 1 cut(s) 271
DdeI CTNAG 4 cut(s) 12, 17, 64, 184
DpnI GATC 3 cut(s) 242, 467, 533
DpnII GATC 3 cut(s) 240, 465, 531
DraIII CACNNNGTG 1 cut(s) 60
EaeI YGGCCR 1 cut(s) 297
Eco130I CCWWGG 6 cut(s) 27, 41, 48, 171, 294, 630
Eco147I AGGCCT 1 cut(s) 189
Eco47I GGWCC 1 cut(s) 45
Eco57I CTGAAG 2 cut(s) 84, 562
Eco88I CYCGRG 2 cut(s) 251, 434
Eco91I GGTNACC 1 cut(s) 401
EcoO65I GGTNACC 1 cut(s) 401
EcoT14I CCWWGG 6 cut(s) 27, 41, 48, 171, 294, 630
ErhI CCWWGG 6 cut(s) 27, 41, 48, 171, 294, 630
FaeI CATG 3 cut(s) 52, 175, 201
FaiI YATR 4 cut(s) 50, 173, 199, 652
FatI CATG 3 cut(s) 48, 171, 197
Fnu4HI GCNGC 3 cut(s) 123, 156, 594
FokI GGATG 2 cut(s) 149, 518
Fsp4HI GCNGC 3 cut(s) 123, 156, 594
FspBI CTAG 1 cut(s) 128
GlaI GCGC 3 cut(s) 154, 227, 286
GluI GCNGC 3 cut(s) 123, 156, 594
GsaI CCCAGC 2 cut(s) 170, 462
HaeIII GGCC 4 cut(s) 32, 189, 299, 586
HapII CCGG 1 cut(s) 435
HhaI GCGC 3 cut(s) 155, 228, 287
Hin1II CATG 3 cut(s) 52, 175, 201
Hin6I GCGC 3 cut(s) 153, 226, 285
HinP1I GCGC 3 cut(s) 153, 226, 285
HinfI GANTC 3 cut(s) 206, 254, 439
HpaII CCGG 1 cut(s) 435
HphI GGTGA 4 cut(s) 413, 431, 622, 629
Hpy166II GTNNAC 1 cut(s) 620
Hpy188I TCNGA 2 cut(s) 373, 572
Hpy188III TCNNGA 1 cut(s) 244
Hpy8I GTNNAC 1 cut(s) 620
HpyAV CCTTC 3 cut(s) 324, 474, 520
HpyCH4III ACNGT 1 cut(s) 58
HpyCH4IV ACGT 1 cut(s) 636
HpyCH4V TGCA 3 cut(s) 125, 201, 308
HpyF10VI GCNNNNNNNGC 4 cut(s) 128, 232, 305, 358
HpyF3I CTNAG 4 cut(s) 12, 17, 64, 184
HpySE526I ACGT 1 cut(s) 636
Hsp92II CATG 3 cut(s) 52, 175, 201
HspAI GCGC 3 cut(s) 153, 226, 285
Kzo9I GATC 3 cut(s) 240, 465, 531
LmnI GCTCC 2 cut(s) 163, 483
Lsp1109I GCAGC 3 cut(s) 109, 167, 580
MaeI CTAG 1 cut(s) 128
MaeII ACGT 1 cut(s) 636
MaeIII GTNAC 3 cut(s) 52, 401, 644
MalI GATC 3 cut(s) 242, 467, 533
MbiI CCGCTC 1 cut(s) 399
MboI GATC 3 cut(s) 240, 465, 531
MboII GAAGA 2 cut(s) 121, 331
MhlI GDGCHC 1 cut(s) 65
MlsI TGGCCA 1 cut(s) 299
MluNI TGGCCA 1 cut(s) 299
MlyI GAGTC 2 cut(s) 200, 263
MmeI TCCRAC 1 cut(s) 457
MnlI CCTC 4 cut(s) 148, 268, 402, 566
Mox20I TGGCCA 1 cut(s) 299
MroXI GAANNNNTTC 1 cut(s) 113
MscI TGGCCA 1 cut(s) 299
MslI CAYNNNNRTG 2 cut(s) 417, 542
Msp20I TGGCCA 1 cut(s) 299
MspI CCGG 1 cut(s) 435
MspR9I CCNGG 2 cut(s) 435, 436
MvnI CGCG 1 cut(s) 228
MwoI GCNNNNNNNGC 4 cut(s) 128, 232, 305, 358
NciI CCSGG 2 cut(s) 435, 436
NcoI CCATGG 2 cut(s) 48, 171
NdeII GATC 3 cut(s) 240, 465, 531
NlaIII CATG 3 cut(s) 52, 175, 201
NmuCI GTSAC 2 cut(s) 52, 401
OliI CACNNNNGTG 1 cut(s) 417
PaeR7I CTCGAG 1 cut(s) 251
PaqCI CACCTGC 1 cut(s) 298
PceI AGGCCT 1 cut(s) 189
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PdmI GAANNNNTTC 1 cut(s) 113
PfeI GAWTC 1 cut(s) 439
PkrI GCNGC 3 cut(s) 124, 157, 595
PleI GAGTC 2 cut(s) 200, 262
PpsI GAGTC 2 cut(s) 200, 262
PshAI GACNNNNGTC 1 cut(s) 50
PspEI GGTNACC 1 cut(s) 401
PspFI CCCAGC 2 cut(s) 166, 458
PspPI GGNCC 3 cut(s) 31, 45, 585
PspXI VCTCGAGB 1 cut(s) 251
RsaI GTAC 1 cut(s) 272
RsaNI GTAC 1 cut(s) 271
RseI CAYNNNNRTG 2 cut(s) 417, 542
SatI GCNGC 3 cut(s) 123, 156, 594
Sau3AI GATC 3 cut(s) 240, 465, 531
Sau96I GGNCC 3 cut(s) 31, 45, 585
SchI GAGTC 2 cut(s) 200, 263
ScrFI CCNGG 2 cut(s) 435, 436
SduI GDGCHC 1 cut(s) 65
SfcI CTRYAG 1 cut(s) 650
Sfr274I CTCGAG 1 cut(s) 251
SinI GGWCC 1 cut(s) 45
SlaI CTCGAG 1 cut(s) 251
SmaI CCCGGG 1 cut(s) 436
SmiMI CAYNNNNRTG 2 cut(s) 417, 542
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
SseBI AGGCCT 1 cut(s) 189
SsiI CCGC 1 cut(s) 399
SspMI CTAG 1 cut(s) 128
StuI AGGCCT 1 cut(s) 189
StyD4I CCNGG 2 cut(s) 433, 434
StyI CCWWGG 6 cut(s) 27, 41, 48, 171, 294, 630
TaaI ACNGT 1 cut(s) 58
TaiI ACGT 1 cut(s) 639
TaqI TCGA 2 cut(s) 239, 252
TfiI GAWTC 1 cut(s) 439
TscAI CASTG 3 cut(s) 61, 100, 419
TseFI GTSAC 2 cut(s) 52, 401
TseI GCWGC 3 cut(s) 122, 155, 593
Tsp45I GTSAC 2 cut(s) 52, 401
TspDTI ATGAA 2 cut(s) 122, 415
TspMI CCCGGG 1 cut(s) 434
TspRI CASTG 3 cut(s) 61, 100, 419
VpaK11BI GGWCC 1 cut(s) 45
XcmI CCANNNNNNNNNTGG 1 cut(s) 169
XhoI CTCGAG 1 cut(s) 251
XmaI CCCGGG 1 cut(s) 434
XmnI GAANNNNTTC 1 cut(s) 113
XspI CTAG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.