MD10G1240300.v1.1

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0019741
Physical Location & Seq
Reverse (-)
33589085 .. 33593653
4569 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1240300.v1.1.491

Sequence Viewer

Length: 1188 bp
ATGAAAACAAAGAGATCATCGAATCCACCAAAGCCAATGTATCATCATCTGCATCTGCCATTCTTCTTCTTCTTCTCGTCTATTTTCTGTCTTATATCATCTGCAAAGGCAATTGCAGAAGCAGAAGCAGAAGCAGCAGTTGGGTATGGATACAAAATTCAATCCGTCCACTATGATTCCTCCGGAAACTCATTGACTGCTGATCTTGGCCTCATCAAGTCCTCCTCCCTCTACGGGCCTGACATCCCAAATCTCAATCTTCACGCCAGCTATGAAACCAAAGATCGTCTAAGAATTCGAATAACGGACTCAAAGAAGCAAAGATGGGAAATCCCACAAGACATGATCCCCCGCCAAACCAAACCCCCTACCCCCCACCAGCACTGCATCTCCCACGACCTTGTCTTCACTCTCCGCAACACCACCCCATTCGGCTTCACCGTCACCCGCTGCTCCTCCAACGACGTCATCTTCGACACCTCCCCCCACTCGTCAGACCCCAACACCTTCTTGGTCTTCAAGGACCAATACATCCAACTCTCCTCTTCTCTCCCCCATAACAGATCCTCCATTTTCGGCCTCGGCGAGCACACCAAGTCCTCCTTCAAGATCATTCCCAATCAGACATTGACTCTCTGGAATGCCGACATTGGTAGCGTCAATCCCGACCTCAACCTTTACGGGTCCCACCCCTTCTACCTGGACGTCAGGTCGGCGTCTCAAGATGGCGGCAAGACCGGTGCAGGAACATCTCATGGAGTGCTGCTGCTGAATAGCAATGGCATGGACATAACATACAGCGGTGATAGGATTACTTATAAGGTCATCGGTGGAATTGTGGACTTGTACTTCTTTGCCGGGCCGACACCTGAGTTGGTAGTGGAGCAGTATACAGAACTAATCGGCCGCCCTGCCCCTATGCCCTACTGGTCTTTTGGATTCCACCAGTGTCGATATGGTTATAAGAATGTTTCCGATCTTGAGGGCGTGGTTGCTGGTTATGCAAAAGCCGGTATCCCTCTGGAAGTTATGTGGACGGACATTGATTACATGGATGCTTACAAGGACTTCACTCTCGATCCCGTCAACTTTCCATTGGATAAGATGAAGAAATTTGTAAGCACTCTTCACCAAAATGGTCAGAAATATGTCCTCATCTTGGATCCTGGTACGCGGCCTAAACCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

396

Amino Acids

44.23

Weight (kDa)

7.25

Isoelectric Point (pI)

33.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gal_mutarotas_2 PF13802 97 - 238 4.8e-09 Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd PF01055 304 - 391 2.1e-31 Glycosyl hydrolases family 31 TIM-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019741)

Species Orthologous Gene IDs
malus_domestica MD10G1240300.v1.1
rosa_chinensis RchiOBHm_Chr5g0018621 RchiOBHm_Chr5g0020521
rosa_multiflora Rmu_co8282331.1_g000001 Rmu_co8283033.1_g000001 Rmu_sc0007192.1_g000001
rosa_samantha Rh5DG136500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 819, 963
AatII GACGTC 2 cut(s) 468, 708
AccI GTMKAC 1 cut(s) 890
AccII CGCG 1 cut(s) 1174
AccIII TCCGGA 1 cut(s) 182
AciI CCGC 7 cut(s) 352, 415, 448, 729, 801, 907, 1174
AclWI GGATC 5 cut(s) 340, 558, 1073, 1157, 1170
AcoI YGGCCR 1 cut(s) 904
AcsI RAATTY 3 cut(s) 156, 294, 1112
AcyI GRCGYC 3 cut(s) 465, 705, 716
AfaI GTAC 2 cut(s) 848, 1171
AfiI CCNNNNNNNGG 2 cut(s) 234, 1159
AgeI ACCGGT 1 cut(s) 737
AgsI TTSAA 3 cut(s) 161, 520, 607
AhdI GACNNNNNGTC 1 cut(s) 709
AjnI CCWGG 2 cut(s) 699, 1165
AjuI GAANNNNNNNTTGG 4 cut(s) 123, 155, 587, 619
AluBI AGCT 1 cut(s) 270
AluI AGCT 1 cut(s) 270
Alw21I GWGCWC 1 cut(s) 591
Alw26I GTCTC 1 cut(s) 723
AlwI GGATC 5 cut(s) 340, 558, 1073, 1157, 1170
Aor13HI TCCGGA 1 cut(s) 182
AoxI GGCC 6 cut(s) 208, 236, 577, 860, 904, 1175
ApeKI GCWGC 4 cut(s) 134, 450, 763, 766
ApoI RAATTY 3 cut(s) 156, 294, 1112
ArsI GACNNNNNNTTYG 2 cut(s) 455, 487
AsiGI ACCGGT 1 cut(s) 737
AspS9I GGNCC 4 cut(s) 236, 523, 684, 860
AsuC2I CCSGG 1 cut(s) 859
AsuHPI GGTGA 4 cut(s) 430, 436, 815, 1121
AsuII TTCGAA 1 cut(s) 298
AvaII GGWCC 2 cut(s) 523, 684
BamHI GGATCC 1 cut(s) 1162
BarI GAAGNNNNNNTAC 2 cut(s) 1110, 1142
BbsI GAAGAC 2 cut(s) 397, 508
Bbv12I GWGCWC 1 cut(s) 591
BbvI GCAGC 4 cut(s) 146, 437, 750, 753
BccI CCATC 2 cut(s) 318, 719
BciT130I CCWGG 2 cut(s) 701, 1167
BciVI GTATCC 2 cut(s) 143, 1025
BcnI CCSGG 1 cut(s) 859
BcoDI GTCTC 1 cut(s) 723
BfuI GTATCC 2 cut(s) 143, 1025
BisI GCNGC 7 cut(s) 135, 451, 730, 764, 767, 907, 1175
BlsI GCNGC 7 cut(s) 136, 452, 731, 765, 768, 908, 1176
Bme1390I CCNGG 3 cut(s) 701, 859, 1167
Bme18I GGWCC 2 cut(s) 523, 684
BmeRI GACNNNNNGTC 1 cut(s) 709
BmgT120I GGNCC 4 cut(s) 236, 523, 684, 860
BmiI GGNNCC 3 cut(s) 685, 686, 1164
BmrFI CCNGG 3 cut(s) 701, 859, 1167
BmsI GCATC 3 cut(s) 61, 396, 1045
BpiI GAAGAC 2 cut(s) 397, 508
Bpu14I TTCGAA 1 cut(s) 298
BpuEI CTTGAG 2 cut(s) 705, 1001
BpuMI CCSGG 1 cut(s) 859
BsaHI GRCGYC 3 cut(s) 465, 705, 716
BsaJI CCNNGG 1 cut(s) 580
BsaWI WCCGGW 2 cut(s) 182, 737
BsaXI ACNNNNNCTCC 4 cut(s) 374, 404, 551, 581
Bsc4I CCNNNNNNNGG 2 cut(s) 234, 1159
Bse118I RCCGGY 2 cut(s) 737, 1010
Bse1I ACTGG 2 cut(s) 932, 946
Bse3DI GCAATG 1 cut(s) 784
BseAI TCCGGA 1 cut(s) 182
BseBI CCWGG 2 cut(s) 701, 1167
BseDI CCNNGG 1 cut(s) 580
BseGI GGATG 3 cut(s) 243, 531, 1060
BseLI CCNNNNNNNGG 2 cut(s) 234, 1159
BseMI GCAATG 1 cut(s) 784
BseMII CTCAG 1 cut(s) 861
BseNI ACTGG 2 cut(s) 932, 946
BseRI GAGGAG 3 cut(s) 214, 445, 532
BseX3I CGGCCG 1 cut(s) 904
BseXI GCAGC 4 cut(s) 146, 437, 750, 753
BsgI GTGCAG 1 cut(s) 762
Bsh1236I CGCG 1 cut(s) 1174
Bsh1285I CGRYCG 1 cut(s) 907
BshFI GGCC 6 cut(s) 210, 238, 579, 862, 906, 1177
BshTI ACCGGT 1 cut(s) 737
BsiEI CGRYCG 1 cut(s) 907
BsiHKAI GWGCWC 1 cut(s) 591
BsiSI CCGG 4 cut(s) 183, 738, 858, 1011
BslFI GGGAC 1 cut(s) 670
BslI CCNNNNNNNGG 2 cut(s) 234, 1159
BsmAI GTCTC 1 cut(s) 723
BsmBI CGTCTC 1 cut(s) 723
BsmFI GGGAC 1 cut(s) 670
BsmI GAATGC 1 cut(s) 646
BsnI GGCC 6 cut(s) 210, 238, 579, 862, 906, 1177
Bsp119I TTCGAA 1 cut(s) 298
Bsp1286I GDGCHC 1 cut(s) 591
Bsp13I TCCGGA 1 cut(s) 182
Bsp143I GATC 9 cut(s) 14, 202, 283, 345, 563, 609, 976, 1078, 1162
BspACI CCGC 7 cut(s) 352, 415, 448, 729, 801, 907, 1174
BspANI GGCC 6 cut(s) 210, 238, 579, 862, 906, 1177
BspCNI CTCAG 1 cut(s) 862
BspEI TCCGGA 1 cut(s) 182
BspFNI CGCG 1 cut(s) 1174
BspLI GGNNCC 3 cut(s) 685, 686, 1164
BspPI GGATC 5 cut(s) 340, 558, 1073, 1157, 1170
BspT104I TTCGAA 1 cut(s) 298
BsrDI GCAATG 1 cut(s) 784
BsrFI RCCGGY 2 cut(s) 737, 1010
BsrI ACTGG 2 cut(s) 932, 946
BssAI RCCGGY 2 cut(s) 737, 1010
BssECI CCNNGG 1 cut(s) 580
BssMI GATC 9 cut(s) 14, 202, 283, 345, 563, 609, 976, 1078, 1162
BssNAI GTATAC 1 cut(s) 891
BssNI GRCGYC 3 cut(s) 465, 705, 716
Bst1107I GTATAC 1 cut(s) 891
Bst2UI CCWGG 2 cut(s) 701, 1167
Bst4CI ACNGT 1 cut(s) 442
Bst6I CTCTTC 2 cut(s) 550, 1131
BstACI GRCGYC 3 cut(s) 465, 705, 716
BstBI TTCGAA 1 cut(s) 298
BstC8I GCNNGC 2 cut(s) 268, 587
BstDEI CTNAG 2 cut(s) 290, 870
BstF5I GGATG 3 cut(s) 243, 531, 1060
BstFNI CGCG 1 cut(s) 1174
BstKTI GATC 9 cut(s) 17, 205, 286, 348, 566, 612, 979, 1081, 1165
BstMAI GTCTC 1 cut(s) 723
BstMBI GATC 9 cut(s) 14, 202, 283, 345, 563, 609, 976, 1078, 1162
BstMCI CGRYCG 1 cut(s) 907
BstMWI GCNNNNNNNGC 2 cut(s) 134, 1001
BstNI CCWGG 2 cut(s) 701, 1167
BstSCI CCNGG 3 cut(s) 699, 857, 1165
BstUI CGCG 1 cut(s) 1174
BstV1I GCAGC 4 cut(s) 146, 437, 750, 753
BstV2I GAAGAC 2 cut(s) 397, 508
BstX2I RGATCY 2 cut(s) 563, 1162
BstYI RGATCY 2 cut(s) 563, 1162
BstZ17I GTATAC 1 cut(s) 891
BstZI CGGCCG 1 cut(s) 904
BsuI GTATCC 2 cut(s) 143, 1025
BsuRI GGCC 6 cut(s) 210, 238, 579, 862, 906, 1177
BtsCI GGATG 3 cut(s) 243, 531, 1060
BtsI GCAGTG 1 cut(s) 382
BtsIMutI CAGTG 2 cut(s) 382, 953
Cac8I GCNNGC 2 cut(s) 268, 587
Cfr10I RCCGGY 2 cut(s) 737, 1010
Cfr13I GGNCC 4 cut(s) 236, 523, 684, 860
CseI GACGC 2 cut(s) 646, 705
Csp6I GTAC 2 cut(s) 847, 1170
CspAI ACCGGT 1 cut(s) 737
CviAII CATG 4 cut(s) 343, 755, 784, 1051
CviQI GTAC 2 cut(s) 847, 1170
DdeI CTNAG 2 cut(s) 290, 870
DpnI GATC 9 cut(s) 16, 204, 285, 347, 565, 611, 978, 1080, 1164
DpnII GATC 9 cut(s) 14, 202, 283, 345, 563, 609, 976, 1078, 1162
DriI GACNNNNNGTC 1 cut(s) 709
EaeI YGGCCR 1 cut(s) 904
EagI CGGCCG 1 cut(s) 904
Eam1104I CTCTTC 2 cut(s) 550, 1131
Eam1105I GACNNNNNGTC 1 cut(s) 709
EarI CTCTTC 2 cut(s) 550, 1131
EclXI CGGCCG 1 cut(s) 904
Eco47I GGWCC 2 cut(s) 523, 684
Eco52I CGGCCG 1 cut(s) 904
EcoO109I RGGNCCY 1 cut(s) 684
EcoRI GAATTC 1 cut(s) 294
EcoRII CCWGG 2 cut(s) 699, 1165
Esp3I CGTCTC 1 cut(s) 723
FaeI CATG 4 cut(s) 346, 758, 787, 1054
FalI AAGNNNNNCTT 2 cut(s) 587, 619
FaqI GGGAC 1 cut(s) 670
FatI CATG 4 cut(s) 342, 754, 783, 1050
FauI CCCGC 2 cut(s) 359, 455
FblI GTMKAC 1 cut(s) 890
Fnu4HI GCNGC 7 cut(s) 135, 451, 730, 764, 767, 907, 1175
FokI GGATG 3 cut(s) 230, 518, 1067
Fsp4HI GCNGC 7 cut(s) 135, 451, 730, 764, 767, 907, 1175
GluI GCNGC 7 cut(s) 135, 451, 730, 764, 767, 907, 1175
HaeIII GGCC 6 cut(s) 210, 238, 579, 862, 906, 1177
HapII CCGG 4 cut(s) 183, 738, 858, 1011
HgaI GACGC 2 cut(s) 646, 705
Hin1I GRCGYC 3 cut(s) 465, 705, 716
Hin1II CATG 4 cut(s) 346, 758, 787, 1054
HincII GTYRAC 1 cut(s) 1087
HindII GTYRAC 1 cut(s) 1087
HinfI GANTC 5 cut(s) 22, 176, 308, 631, 939
HpaII CCGG 4 cut(s) 183, 738, 858, 1011
HphI GGTGA 4 cut(s) 430, 436, 815, 1121
Hpy166II GTNNAC 5 cut(s) 169, 841, 891, 1035, 1087
Hpy188I TCNGA 4 cut(s) 496, 624, 976, 1143
Hpy188III TCNNGA 8 cut(s) 183, 607, 637, 665, 722, 980, 1022, 1076
Hpy8I GTNNAC 5 cut(s) 169, 841, 891, 1035, 1087
Hpy99I CGWCG 1 cut(s) 467
HpyAV CCTTC 3 cut(s) 517, 613, 703
HpyCH4III ACNGT 1 cut(s) 442
HpyCH4IV ACGT 2 cut(s) 465, 705
HpyCH4V TGCA 6 cut(s) 52, 104, 116, 387, 743, 1004
HpyF10VI GCNNNNNNNGC 2 cut(s) 134, 1001
HpyF3I CTNAG 2 cut(s) 290, 870
HpySE526I ACGT 2 cut(s) 465, 705
Hsp92I GRCGYC 3 cut(s) 465, 705, 716
Hsp92II CATG 4 cut(s) 346, 758, 787, 1054
KflI GGGWCCC 1 cut(s) 684
Kpn2I TCCGGA 1 cut(s) 182
Kzo9I GATC 9 cut(s) 14, 202, 283, 345, 563, 609, 976, 1078, 1162
LmnI GCTCC 2 cut(s) 458, 883
Lsp1109I GCAGC 4 cut(s) 146, 437, 750, 753
LweI GCATC 3 cut(s) 61, 396, 1045
MaeII ACGT 2 cut(s) 465, 705
MaeIII GTNAC 1 cut(s) 442
MalI GATC 9 cut(s) 16, 204, 285, 347, 565, 611, 978, 1080, 1164
MboI GATC 9 cut(s) 14, 202, 283, 345, 563, 609, 976, 1078, 1162
MfeI CAATTG 1 cut(s) 111
MflI RGATCY 2 cut(s) 563, 1162
MhlI GDGCHC 1 cut(s) 591
MluCI AATT 5 cut(s) 111, 156, 294, 834, 1112
MlyI GAGTC 2 cut(s) 302, 625
MmeI TCCRAC 2 cut(s) 483, 559
MroI TCCGGA 1 cut(s) 182
MslI CAYNNNNRTG 1 cut(s) 1134
MspA1I CMGCKG 2 cut(s) 450, 801
MspI CCGG 4 cut(s) 183, 738, 858, 1011
MspR9I CCNGG 3 cut(s) 701, 859, 1167
MunI CAATTG 1 cut(s) 111
Mva1269I GAATGC 1 cut(s) 646
MvaI CCWGG 2 cut(s) 701, 1167
MvnI CGCG 1 cut(s) 1174
MwoI GCNNNNNNNGC 2 cut(s) 134, 1001
NciI CCSGG 1 cut(s) 859
NdeII GATC 9 cut(s) 14, 202, 283, 345, 563, 609, 976, 1078, 1162
NlaIII CATG 4 cut(s) 346, 758, 787, 1054
NlaIV GGNNCC 3 cut(s) 685, 686, 1164
NmeAIII GCCGAG 1 cut(s) 561
NmuCI GTSAC 1 cut(s) 442
NspV TTCGAA 1 cut(s) 298
PcsI WCGNNNNNNNCGW 3 cut(s) 438, 471, 582
PctI GAATGC 1 cut(s) 646
PfeI GAWTC 3 cut(s) 22, 176, 939
PflFI GACNNNGTC 1 cut(s) 401
PinAI ACCGGT 1 cut(s) 737
PkrI GCNGC 7 cut(s) 136, 452, 731, 765, 768, 908, 1176
PleI GAGTC 2 cut(s) 302, 625
PpsI GAGTC 2 cut(s) 302, 625
PpuMI RGGWCCY 1 cut(s) 684
PsiI TTATAA 2 cut(s) 819, 963
Psp5II RGGWCCY 1 cut(s) 684
Psp6I CCWGG 2 cut(s) 699, 1165
PspGI CCWGG 2 cut(s) 699, 1165
PspN4I GGNNCC 3 cut(s) 685, 686, 1164
PspPI GGNCC 4 cut(s) 236, 523, 684, 860
PspPPI RGGWCCY 1 cut(s) 684
PsuI RGATCY 2 cut(s) 563, 1162
PsyI GACNNNGTC 1 cut(s) 401
RsaI GTAC 2 cut(s) 848, 1171
RsaNI GTAC 2 cut(s) 847, 1170
RseI CAYNNNNRTG 1 cut(s) 1134
SatI GCNGC 7 cut(s) 135, 451, 730, 764, 767, 907, 1175
Sau3AI GATC 9 cut(s) 14, 202, 283, 345, 563, 609, 976, 1078, 1162
Sau96I GGNCC 4 cut(s) 236, 523, 684, 860
SchI GAGTC 2 cut(s) 302, 625
ScrFI CCNGG 3 cut(s) 701, 859, 1167
SduI GDGCHC 1 cut(s) 591
SfaNI GCATC 3 cut(s) 61, 396, 1045
SfuI TTCGAA 1 cut(s) 298
SinI GGWCC 2 cut(s) 523, 684
SmiMI CAYNNNNRTG 1 cut(s) 1134
SmlI CTYRAG 2 cut(s) 720, 980
SmoI CTYRAG 2 cut(s) 720, 980
Sse9I AATT 5 cut(s) 111, 156, 294, 834, 1112
SsiI CCGC 7 cut(s) 352, 415, 448, 729, 801, 907, 1174
StyD4I CCNGG 3 cut(s) 699, 857, 1165
TaaI ACNGT 1 cut(s) 442
TaiI ACGT 2 cut(s) 468, 708
TaqI TCGA 5 cut(s) 20, 298, 474, 952, 1077
TasI AATT 5 cut(s) 111, 156, 294, 834, 1112
TatI WGTACW 1 cut(s) 846
TauI GCSGC 3 cut(s) 732, 909, 1177
TfiI GAWTC 3 cut(s) 22, 176, 939
TscAI CASTG 2 cut(s) 389, 953
TseFI GTSAC 1 cut(s) 442
TseI GCWGC 4 cut(s) 134, 450, 763, 766
Tsp45I GTSAC 1 cut(s) 442
TspDTI ATGAA 3 cut(s) 17, 288, 1121
TspGWI ACGGA 3 cut(s) 154, 320, 1052
TspRI CASTG 2 cut(s) 389, 953
Tth111I GACNNNGTC 1 cut(s) 401
VpaK11BI GGWCC 2 cut(s) 523, 684
XapI RAATTY 3 cut(s) 156, 294, 1112
XcmI CCANNNNNNNNNTGG 2 cut(s) 508, 953
XmiI GTMKAC 1 cut(s) 890
ZraI GACGTC 2 cut(s) 466, 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.