RchiOBHm_Chr5g0020521

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0019741
Physical Location & Seq
Reverse (-)
14743618 .. 14744729
1112 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30060

Sequence Viewer

Length: 963 bp
ATGGAAAGAAACCGATCAAAATACACACCAAAAGCCACATTCCATCATCTTCTACCATTCTTCTTCTTCTATATCTTCTTCTCTCTTGATTGTGTAGTTGCACAAGAGTTAGTTGGGTATGGATATAAAGTCGAGTCTGTGAATAATTCTGATTCCTCGGGGAAGACATTGACCGTTAACCTTGGTCTCATCAACAGCTCCTCAGTTTATGGACGTGACATATCAAATCTTACCCTCTATGTCAGCTATGAGACCAAAGACCGTCTAAGAGTTCGAATAACAGACTCAAAGCAACAAAGATGGGAGCTCCCACAAGACATTCTCCCCCGCCAAAACCAAACTTCACAGAACTTTTCCGTCACTGACAACGACCTCGTTTTCACCCTCCGCAACACCACCCCGTTCGGCTTCACCATCACCCGCCGCTCCTCTAACGACACCCTTTTCGACACCACCCCCAACCCCTCCGACCCCAACACCACCTTCATCTTCAAGGACCAATACTTCCAGCTCTCCTCCTCTCTTCCCAACAACAGGTCCTCCCTGTACGGCCTCGGAGAGCACACCAAGAGCACCTTCAAGCTCCTAGCCAATCAGACTCTGACACTCTGGAGCGCCGACATCGGCAGCGTGAGTCCCGACCTCAATCTCTATGGGTCCCACCCCTTCTACATGGACGTAAGGTCGCCGTCCGGTGATGGCAAGGTCAAGGCGGGAACCACTCACGGAGTGCTGCTTTTGAACAGCAATGGCATGGATGTAAATTACACCGGTGACAGGGTTACTTATAATGTGATTGGCGGAGTTGTGGACTTGTATTTCTTTTCGGGTCCGACACCCGAATTGGTGATGGAGCAGTACACTGAGCTCATTGGTCGACCCGCCCCTATGCCATATTGGTCTTTCGGTAAGTTGTCTATATTCTTTTCTGGCAATTGTAAATTTCATTCATCAAATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

320

Amino Acids

36.05

Weight (kDa)

7.01

Isoelectric Point (pI)

36.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gal_mutarotas_2 PF13802 82 - 250 2.5e-11 Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019741)

Species Orthologous Gene IDs
malus_domestica MD10G1240300.v1.1
rosa_chinensis RchiOBHm_Chr5g0018621 RchiOBHm_Chr5g0020521
rosa_multiflora Rmu_co8282331.1_g000001 Rmu_co8283033.1_g000001 Rmu_sc0007192.1_g000001
rosa_samantha Rh5DG136500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 789
AccBSI CCGCTC 1 cut(s) 426
AccI GTMKAC 1 cut(s) 877
AciI CCGC 7 cut(s) 328, 388, 421, 424, 713, 801, 882
AcsI RAATTY 1 cut(s) 941
AdeI CACNNNGTG 1 cut(s) 730
AfaI GTAC 2 cut(s) 548, 860
AfiI CCNNNNNNNGG 2 cut(s) 534, 777
AgeI ACCGGT 1 cut(s) 770
AgsI TTSAA 3 cut(s) 493, 580, 742
AhdI GACNNNNNGTC 1 cut(s) 682
AjiI CACGTC 1 cut(s) 215
AjuI GAANNNNNNNTTGG 4 cut(s) 467, 499, 560, 592
AluBI AGCT 6 cut(s) 198, 246, 307, 511, 583, 868
AluI AGCT 6 cut(s) 198, 246, 307, 511, 583, 868
Alw21I GWGCWC 4 cut(s) 309, 564, 575, 870
Alw26I GTCTC 2 cut(s) 191, 245
AlwNI CAGNNNCTG 1 cut(s) 601
Ama87I CYCGRG 1 cut(s) 157
AoxI GGCC 1 cut(s) 550
ApeKI GCWGC 2 cut(s) 627, 733
ApoI RAATTY 1 cut(s) 941
ArsI GACNNNNNNTTYG 2 cut(s) 428, 460
AsiGI ACCGGT 1 cut(s) 770
AspLEI GCGC 1 cut(s) 617
AspS9I GGNCC 4 cut(s) 496, 537, 657, 830
AsuHPI GGTGA 6 cut(s) 373, 403, 409, 707, 785, 859
AsuII TTCGAA 1 cut(s) 274
AvaI CYCGRG 1 cut(s) 157
AvaII GGWCC 4 cut(s) 496, 537, 657, 830
BanII GRGCYC 2 cut(s) 309, 870
BbsI GAAGAC 1 cut(s) 170
Bbv12I GWGCWC 4 cut(s) 309, 564, 575, 870
BbvI GCAGC 2 cut(s) 639, 720
BccI CCATC 5 cut(s) 51, 294, 422, 692, 844
BceAI ACGGC 2 cut(s) 565, 673
BcoDI GTCTC 2 cut(s) 191, 245
BfaI CTAG 1 cut(s) 587
BfoI RGCGCY 1 cut(s) 618
BisI GCNGC 3 cut(s) 424, 628, 734
BlsI GCNGC 3 cut(s) 425, 629, 735
Bme18I GGWCC 4 cut(s) 496, 537, 657, 830
BmeRI GACNNNNNGTC 1 cut(s) 682
BmeT110I CYCGRG 1 cut(s) 157
BmgBI CACGTC 1 cut(s) 215
BmgT120I GGNCC 4 cut(s) 496, 537, 657, 830
BmiI GGNNCC 4 cut(s) 658, 659, 718, 831
BpiI GAAGAC 1 cut(s) 170
BpmI CTGGAG 1 cut(s) 631
Bpu14I TTCGAA 1 cut(s) 274
BsaI GGTCTC 2 cut(s) 191, 245
BsaJI CCNNGG 3 cut(s) 156, 181, 553
BsaWI WCCGGW 2 cut(s) 692, 770
BsaXI ACNNNNNCTCC 2 cut(s) 524, 554
Bsc4I CCNNNNNNNGG 2 cut(s) 534, 777
Bse118I RCCGGY 1 cut(s) 770
Bse3DI GCAATG 1 cut(s) 754
BseDI CCNNGG 3 cut(s) 156, 181, 553
BseGI GGATG 1 cut(s) 763
BseLI CCNNNNNNNGG 2 cut(s) 534, 777
BseMI GCAATG 1 cut(s) 754
BseMII CTCAG 2 cut(s) 216, 855
BseRI GAGGAG 4 cut(s) 190, 418, 505, 508
BseXI GCAGC 2 cut(s) 639, 720
BshFI GGCC 1 cut(s) 552
BshTI ACCGGT 1 cut(s) 770
BsiHKAI GWGCWC 4 cut(s) 309, 564, 575, 870
BsiHKCI CYCGRG 1 cut(s) 157
BsiSI CCGG 2 cut(s) 693, 771
BslFI GGGAC 2 cut(s) 621, 643
BslI CCNNNNNNNGG 2 cut(s) 534, 777
BsmAI GTCTC 2 cut(s) 191, 245
BsmFI GGGAC 2 cut(s) 621, 643
BsnI GGCC 1 cut(s) 552
Bso31I GGTCTC 2 cut(s) 191, 245
BsoBI CYCGRG 1 cut(s) 157
Bsp119I TTCGAA 1 cut(s) 274
Bsp1286I GDGCHC 4 cut(s) 309, 564, 575, 870
Bsp143I GATC 1 cut(s) 14
BspACI CCGC 7 cut(s) 328, 388, 421, 424, 713, 801, 882
BspANI GGCC 1 cut(s) 552
BspCNI CTCAG 2 cut(s) 215, 856
BspLI GGNNCC 4 cut(s) 658, 659, 718, 831
BspT104I TTCGAA 1 cut(s) 274
BspTNI GGTCTC 2 cut(s) 191, 245
BsrBI CCGCTC 1 cut(s) 426
BsrDI GCAATG 1 cut(s) 754
BsrFI RCCGGY 1 cut(s) 770
BssAI RCCGGY 1 cut(s) 770
BssECI CCNNGG 3 cut(s) 156, 181, 553
BssMI GATC 1 cut(s) 14
BssT1I CCWWGG 1 cut(s) 181
Bst4CI ACNGT 2 cut(s) 175, 263
Bst6I CTCTTC 1 cut(s) 528
BstBI TTCGAA 1 cut(s) 274
BstDEI CTNAG 3 cut(s) 202, 266, 864
BstF5I GGATG 1 cut(s) 763
BstH2I RGCGCY 1 cut(s) 618
BstHHI GCGC 1 cut(s) 617
BstKTI GATC 1 cut(s) 17
BstMAI GTCTC 2 cut(s) 191, 245
BstMBI GATC 1 cut(s) 14
BstV1I GCAGC 2 cut(s) 639, 720
BstV2I GAAGAC 1 cut(s) 170
BsuRI GGCC 1 cut(s) 552
BtrI CACGTC 1 cut(s) 215
BtsCI GGATG 1 cut(s) 763
BtsIMutI CAGTG 2 cut(s) 360, 861
CaiI CAGNNNCTG 1 cut(s) 601
CfoI GCGC 1 cut(s) 617
Cfr10I RCCGGY 1 cut(s) 770
Cfr13I GGNCC 4 cut(s) 496, 537, 657, 830
Csp6I GTAC 2 cut(s) 547, 859
CspAI ACCGGT 1 cut(s) 770
CviAII CATG 2 cut(s) 673, 754
CviQI GTAC 2 cut(s) 547, 859
DdeI CTNAG 3 cut(s) 202, 266, 864
DpnI GATC 1 cut(s) 16
DpnII GATC 1 cut(s) 14
DraIII CACNNNGTG 1 cut(s) 730
DriI GACNNNNNGTC 1 cut(s) 682
Eam1104I CTCTTC 1 cut(s) 528
Eam1105I GACNNNNNGTC 1 cut(s) 682
EarI CTCTTC 1 cut(s) 528
EciI GGCGGA 1 cut(s) 816
Ecl136II GAGCTC 2 cut(s) 307, 868
Eco130I CCWWGG 1 cut(s) 181
Eco24I GRGCYC 2 cut(s) 309, 870
Eco31I GGTCTC 2 cut(s) 191, 245
Eco47I GGWCC 4 cut(s) 496, 537, 657, 830
Eco53kI GAGCTC 2 cut(s) 307, 868
Eco88I CYCGRG 1 cut(s) 157
EcoICRI GAGCTC 2 cut(s) 307, 868
EcoO109I RGGNCCY 2 cut(s) 537, 657
EcoT14I CCWWGG 1 cut(s) 181
EcoT38I GRGCYC 2 cut(s) 309, 870
ErhI CCWWGG 1 cut(s) 181
FaeI CATG 2 cut(s) 676, 757
FalI AAGNNNNNCTT 2 cut(s) 560, 592
FaqI GGGAC 2 cut(s) 621, 643
FatI CATG 2 cut(s) 672, 753
FauI CCCGC 4 cut(s) 335, 428, 706, 889
FblI GTMKAC 1 cut(s) 877
Fnu4HI GCNGC 3 cut(s) 424, 628, 734
FokI GGATG 1 cut(s) 770
FriOI GRGCYC 2 cut(s) 309, 870
Fsp4HI GCNGC 3 cut(s) 424, 628, 734
FspBI CTAG 1 cut(s) 587
GlaI GCGC 1 cut(s) 616
GluI GCNGC 3 cut(s) 424, 628, 734
GsuI CTGGAG 1 cut(s) 631
HaeII RGCGCY 1 cut(s) 618
HaeIII GGCC 1 cut(s) 552
HapII CCGG 2 cut(s) 693, 771
HhaI GCGC 1 cut(s) 617
Hin1II CATG 2 cut(s) 676, 757
Hin6I GCGC 1 cut(s) 615
HinP1I GCGC 1 cut(s) 615
HincII GTYRAC 2 cut(s) 178, 878
HindII GTYRAC 2 cut(s) 178, 878
HinfI GANTC 5 cut(s) 134, 152, 284, 598, 634
HpaI GTTAAC 1 cut(s) 178
HpaII CCGG 2 cut(s) 693, 771
HphI GGTGA 6 cut(s) 373, 403, 409, 707, 785, 859
Hpy166II GTNNAC 4 cut(s) 178, 811, 861, 878
Hpy188I TCNGA 6 cut(s) 151, 469, 557, 597, 603, 834
Hpy188III TCNNGA 3 cut(s) 86, 610, 638
Hpy8I GTNNAC 4 cut(s) 178, 811, 861, 878
HpyAV CCTTC 3 cut(s) 493, 586, 676
HpyCH4III ACNGT 2 cut(s) 175, 263
HpyCH4IV ACGT 2 cut(s) 214, 678
HpyCH4V TGCA 1 cut(s) 101
HpyF3I CTNAG 3 cut(s) 202, 266, 864
HpySE526I ACGT 2 cut(s) 214, 678
Hsp92II CATG 2 cut(s) 676, 757
HspAI GCGC 1 cut(s) 615
KflI GGGWCCC 1 cut(s) 657
KspAI GTTAAC 1 cut(s) 178
Kzo9I GATC 1 cut(s) 14
LmnI GCTCC 7 cut(s) 203, 304, 312, 431, 588, 612, 853
LpnPI CCDG 8 cut(s) 520, 521, 557, 595, 706, 763, 784, 915
Lsp1109I GCAGC 2 cut(s) 639, 720
MaeI CTAG 1 cut(s) 587
MaeII ACGT 2 cut(s) 214, 678
MaeIII GTNAC 4 cut(s) 215, 358, 773, 781
MalI GATC 1 cut(s) 16
MbiI CCGCTC 1 cut(s) 426
MboI GATC 1 cut(s) 14
MboII GAAGA 9 cut(s) 41, 52, 55, 58, 67, 70, 175, 481, 515
MfeI CAATTG 1 cut(s) 934
MhlI GDGCHC 4 cut(s) 309, 564, 575, 870
MluCI AATT 5 cut(s) 145, 763, 842, 934, 941
MlyI GAGTC 4 cut(s) 143, 278, 592, 643
MmeI TCCRAC 2 cut(s) 492, 857
MseI TTAA 2 cut(s) 177, 961
MspI CCGG 2 cut(s) 693, 771
MunI CAATTG 1 cut(s) 934
NdeII GATC 1 cut(s) 14
NlaIII CATG 2 cut(s) 676, 757
NlaIV GGNNCC 4 cut(s) 658, 659, 718, 831
NmuCI GTSAC 3 cut(s) 215, 358, 773
NspV TTCGAA 1 cut(s) 274
PfeI GAWTC 1 cut(s) 152
PinAI ACCGGT 1 cut(s) 770
PkrI GCNGC 3 cut(s) 425, 629, 735
PleI GAGTC 4 cut(s) 142, 278, 592, 642
PpsI GAGTC 4 cut(s) 142, 278, 592, 642
PpuMI RGGWCCY 2 cut(s) 537, 657
PsiI TTATAA 1 cut(s) 789
Psp124BI GAGCTC 2 cut(s) 309, 870
Psp5II RGGWCCY 2 cut(s) 537, 657
PspN4I GGNNCC 4 cut(s) 658, 659, 718, 831
PspPI GGNCC 4 cut(s) 496, 537, 657, 830
PspPPI RGGWCCY 2 cut(s) 537, 657
PstNI CAGNNNCTG 1 cut(s) 601
RsaI GTAC 2 cut(s) 548, 860
RsaNI GTAC 2 cut(s) 547, 859
SacI GAGCTC 2 cut(s) 309, 870
SalI GTCGAC 1 cut(s) 876
SaqAI TTAA 2 cut(s) 177, 961
SatI GCNGC 3 cut(s) 424, 628, 734
Sau3AI GATC 1 cut(s) 14
Sau96I GGNCC 4 cut(s) 496, 537, 657, 830
SchI GAGTC 4 cut(s) 143, 278, 592, 643
SduI GDGCHC 4 cut(s) 309, 564, 575, 870
SfuI TTCGAA 1 cut(s) 274
SgrAI CRCCGGYG 1 cut(s) 770
SinI GGWCC 4 cut(s) 496, 537, 657, 830
Sse9I AATT 5 cut(s) 145, 763, 842, 934, 941
SsiI CCGC 7 cut(s) 328, 388, 421, 424, 713, 801, 882
SspMI CTAG 1 cut(s) 587
SstI GAGCTC 2 cut(s) 309, 870
StyI CCWWGG 1 cut(s) 181
TaaI ACNGT 2 cut(s) 175, 263
TaiI ACGT 2 cut(s) 217, 681
TaqI TCGA 4 cut(s) 132, 274, 447, 877
TasI AATT 5 cut(s) 145, 763, 842, 934, 941
TatI WGTACW 1 cut(s) 858
TauI GCSGC 1 cut(s) 426
TfiI GAWTC 1 cut(s) 152
Tru1I TTAA 2 cut(s) 177, 961
Tru9I TTAA 2 cut(s) 177, 961
TscAI CASTG 2 cut(s) 367, 868
TseFI GTSAC 3 cut(s) 215, 358, 773
TseI GCWGC 2 cut(s) 627, 733
Tsp45I GTSAC 3 cut(s) 215, 358, 773
TspDTI ATGAA 3 cut(s) 475, 935, 939
TspGWI ACGGA 2 cut(s) 346, 741
TspRI CASTG 2 cut(s) 367, 868
VpaK11BI GGWCC 4 cut(s) 496, 537, 657, 830
XapI RAATTY 1 cut(s) 941
XmiI GTMKAC 1 cut(s) 877
XspI CTAG 1 cut(s) 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.