RchiOBHm_Chr5g0018621

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0019741
Physical Location & Seq
Forward (+)
13150269 .. 13150844
576 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29880

Sequence Viewer

Length: 576 bp
ATGGTTAATGTTTTGAATCAATATTTACCAACATGTTCTATAATCAACAGCTATGAAACCAAAGAAAGACTAAGAATTCGAATAACAGACTCGAGTAACCAAGGATGGGAAATCCCACAAGACCTTCTCCCTCGACAAACTCCACAAACATCCCACCATTCGATCCCCGAAAACGATCTCATTTTCACCCTCCACACCACCACCCCGTTCGGCTTCACTGTCACCCGCCGCTTCTCCAATGATGTTGCATTCGACGCCACCCCAAACCCCTCCAACCCTTCCTCCATCCTCGTCTTCAAGGACCAGTACATCCAGCTTTCCTCTTCTCTCCCGAAAACCCGATCTTCACTGTACGGCCTCGGTGAGCACACCAAAGCCTCCTTCAAGCTCCAATCCAATCAGACTCTAACACTCTGGACCGCCGACATTTTCAGCTCCATCCCCGACCTCAATCTCTATGGGTCCCACCCATTTTACATGGACGTGAGGTCATCGTCCGGTGATGGTAAAGTTAATGCAGGAACCACTATATCATGGCATGGATGTAAATTACGACGGCGACAGGATTACATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

191

Amino Acids

21.65

Weight (kDa)

8.57

Isoelectric Point (pI)

49.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gal_mutarotas_2 PF13802 32 - 165 2e-10 Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019741)

Species Orthologous Gene IDs
malus_domestica MD10G1240300.v1.1
rosa_chinensis RchiOBHm_Chr5g0018621 RchiOBHm_Chr5g0020521
rosa_multiflora Rmu_co8282331.1_g000001 Rmu_co8283033.1_g000001 Rmu_sc0007192.1_g000001
rosa_samantha Rh5DG136500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 226, 229, 420
AclWI GGATC 1 cut(s) 157
AcsI RAATTY 1 cut(s) 75
AcyI GRCGYC 1 cut(s) 255
AfaI GTAC 2 cut(s) 308, 353
AfiI CCNNNNNNNGG 1 cut(s) 106
AflIII ACRYGT 1 cut(s) 32
AgsI TTSAA 3 cut(s) 16, 298, 385
AhdI GACNNNNNGTC 1 cut(s) 487
AjiI CACGTC 1 cut(s) 484
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
AluBI AGCT 4 cut(s) 51, 316, 388, 435
AluI AGCT 4 cut(s) 51, 316, 388, 435
Alw21I GWGCWC 1 cut(s) 369
AlwI GGATC 1 cut(s) 157
Ama87I CYCGRG 1 cut(s) 91
AoxI GGCC 1 cut(s) 355
ApoI RAATTY 1 cut(s) 75
ArsI GACNNNNNNTTYG 2 cut(s) 60, 92
AspS9I GGNCC 3 cut(s) 301, 417, 462
AsuHPI GGTGA 4 cut(s) 178, 214, 374, 512
AsuII TTCGAA 1 cut(s) 79
AvaI CYCGRG 1 cut(s) 91
AvaII GGWCC 3 cut(s) 301, 417, 462
BbsI GAAGAC 1 cut(s) 286
Bbv12I GWGCWC 1 cut(s) 369
BccI CCATC 4 cut(s) 99, 293, 446, 497
BceAI ACGGC 2 cut(s) 370, 572
BisI GCNGC 1 cut(s) 229
BlsI GCNGC 1 cut(s) 230
Bme18I GGWCC 3 cut(s) 301, 417, 462
BmeRI GACNNNNNGTC 1 cut(s) 487
BmeT110I CYCGRG 1 cut(s) 91
BmgBI CACGTC 1 cut(s) 484
BmgT120I GGNCC 3 cut(s) 301, 417, 462
BmiI GGNNCC 3 cut(s) 463, 464, 523
BpiI GAAGAC 1 cut(s) 286
Bpu14I TTCGAA 1 cut(s) 79
BsaHI GRCGYC 1 cut(s) 255
BsaJI CCNNGG 2 cut(s) 100, 358
BsaWI WCCGGW 1 cut(s) 497
BsaXI ACNNNNNCTCC 2 cut(s) 266, 296
Bsc4I CCNNNNNNNGG 1 cut(s) 106
Bse1I ACTGG 1 cut(s) 304
BseDI CCNNGG 2 cut(s) 100, 358
BseGI GGATG 6 cut(s) 110, 149, 285, 309, 438, 548
BseLI CCNNNNNNNGG 1 cut(s) 106
BseNI ACTGG 1 cut(s) 304
BshFI GGCC 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 369
BsiHKCI CYCGRG 1 cut(s) 91
BsiSI CCGG 1 cut(s) 498
BslFI GGGAC 1 cut(s) 448
BslI CCNNNNNNNGG 1 cut(s) 106
BsmFI GGGAC 1 cut(s) 448
BsmI GAATGC 1 cut(s) 248
BsnI GGCC 1 cut(s) 357
BsoBI CYCGRG 1 cut(s) 91
Bsp119I TTCGAA 1 cut(s) 79
Bsp1286I GDGCHC 1 cut(s) 369
Bsp143I GATC 3 cut(s) 162, 175, 341
BspACI CCGC 3 cut(s) 226, 229, 420
BspANI GGCC 1 cut(s) 357
BspLI GGNNCC 3 cut(s) 463, 464, 523
BspPI GGATC 1 cut(s) 157
BspT104I TTCGAA 1 cut(s) 79
BsrI ACTGG 1 cut(s) 304
BssECI CCNNGG 2 cut(s) 100, 358
BssMI GATC 3 cut(s) 162, 175, 341
BssNI GRCGYC 1 cut(s) 255
BssT1I CCWWGG 1 cut(s) 100
Bst4CI ACNGT 2 cut(s) 220, 351
Bst6I CTCTTC 1 cut(s) 328
BstACI GRCGYC 1 cut(s) 255
BstBI TTCGAA 1 cut(s) 79
BstDEI CTNAG 1 cut(s) 71
BstF5I GGATG 6 cut(s) 110, 149, 285, 309, 438, 548
BstKTI GATC 3 cut(s) 165, 178, 344
BstMBI GATC 3 cut(s) 162, 175, 341
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstNSI RCATGY 1 cut(s) 36
BstV2I GAAGAC 1 cut(s) 286
BsuRI GGCC 1 cut(s) 357
BtrI CACGTC 1 cut(s) 484
BtsCI GGATG 6 cut(s) 110, 149, 285, 309, 438, 548
BtsIMutI CAGTG 2 cut(s) 216, 347
Cfr13I GGNCC 3 cut(s) 301, 417, 462
CseI GACGC 1 cut(s) 263
Csp6I GTAC 2 cut(s) 307, 352
CviAII CATG 4 cut(s) 33, 478, 534, 539
CviJI RGCY 7 cut(s) 51, 213, 316, 357, 377, 388, 435
CviKI_1 RGCY 7 cut(s) 51, 213, 316, 357, 377, 388, 435
CviQI GTAC 2 cut(s) 307, 352
DdeI CTNAG 1 cut(s) 71
DpnI GATC 3 cut(s) 164, 177, 343
DpnII GATC 3 cut(s) 162, 175, 341
DriI GACNNNNNGTC 1 cut(s) 487
Eam1104I CTCTTC 1 cut(s) 328
Eam1105I GACNNNNNGTC 1 cut(s) 487
EarI CTCTTC 1 cut(s) 328
Eco130I CCWWGG 1 cut(s) 100
Eco47I GGWCC 3 cut(s) 301, 417, 462
Eco88I CYCGRG 1 cut(s) 91
EcoO109I RGGNCCY 1 cut(s) 462
EcoRI GAATTC 1 cut(s) 75
EcoT14I CCWWGG 1 cut(s) 100
ErhI CCWWGG 1 cut(s) 100
FaeI CATG 4 cut(s) 36, 481, 537, 542
FaqI GGGAC 1 cut(s) 448
FatI CATG 4 cut(s) 32, 477, 533, 538
FauI CCCGC 1 cut(s) 233
Fnu4HI GCNGC 1 cut(s) 229
FokI GGATG 6 cut(s) 117, 136, 272, 296, 425, 555
Fsp4HI GCNGC 1 cut(s) 229
GluI GCNGC 1 cut(s) 229
HaeIII GGCC 1 cut(s) 357
HapII CCGG 1 cut(s) 498
HgaI GACGC 1 cut(s) 263
Hin1I GRCGYC 1 cut(s) 255
Hin1II CATG 4 cut(s) 36, 481, 537, 542
HinfI GANTC 3 cut(s) 16, 89, 403
HpaII CCGG 1 cut(s) 498
HphI GGTGA 4 cut(s) 178, 214, 374, 512
Hpy188I TCNGA 1 cut(s) 402
Hpy188III TCNNGA 2 cut(s) 331, 415
Hpy99I CGWCG 2 cut(s) 257, 558
HpyAV CCTTC 3 cut(s) 134, 288, 391
HpyCH4III ACNGT 2 cut(s) 220, 351
HpyCH4IV ACGT 1 cut(s) 483
HpyCH4V TGCA 2 cut(s) 248, 518
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpyF3I CTNAG 1 cut(s) 71
HpySE526I ACGT 1 cut(s) 483
Hsp92I GRCGYC 1 cut(s) 255
Hsp92II CATG 4 cut(s) 36, 481, 537, 542
KflI GGGWCCC 1 cut(s) 462
Kzo9I GATC 3 cut(s) 162, 175, 341
LmnI GCTCC 2 cut(s) 393, 440
LpnPI CCDG 6 cut(s) 317, 326, 400, 504, 511, 548
MaeII ACGT 1 cut(s) 483
MaeIII GTNAC 2 cut(s) 95, 220
MalI GATC 3 cut(s) 164, 177, 343
MboI GATC 3 cut(s) 162, 175, 341
MboII GAAGA 3 cut(s) 286, 315, 336
MhlI GDGCHC 1 cut(s) 369
MluCI AATT 2 cut(s) 75, 548
MlyI GAGTC 2 cut(s) 83, 397
MmeI TCCRAC 1 cut(s) 297
MseI TTAA 2 cut(s) 6, 513
MslI CAYNNNNRTG 1 cut(s) 482
MspI CCGG 1 cut(s) 498
Mva1269I GAATGC 1 cut(s) 248
MwoI GCNNNNNNNGC 1 cut(s) 254
NdeII GATC 3 cut(s) 162, 175, 341
NlaIII CATG 4 cut(s) 36, 481, 537, 542
NlaIV GGNNCC 3 cut(s) 463, 464, 523
NmuCI GTSAC 1 cut(s) 220
NspI RCATGY 1 cut(s) 36
NspV TTCGAA 1 cut(s) 79
PaeR7I CTCGAG 1 cut(s) 91
PciI ACATGT 1 cut(s) 32
PctI GAATGC 1 cut(s) 248
PfeI GAWTC 1 cut(s) 16
PkrI GCNGC 1 cut(s) 230
PleI GAGTC 2 cut(s) 83, 397
PpsI GAGTC 2 cut(s) 83, 397
PpuMI RGGWCCY 1 cut(s) 462
PscI ACATGT 1 cut(s) 32
Psp5II RGGWCCY 1 cut(s) 462
PspN4I GGNNCC 3 cut(s) 463, 464, 523
PspPI GGNCC 3 cut(s) 301, 417, 462
PspPPI RGGWCCY 1 cut(s) 462
PspXI VCTCGAGB 1 cut(s) 91
RsaI GTAC 2 cut(s) 308, 353
RsaNI GTAC 2 cut(s) 307, 352
RseI CAYNNNNRTG 1 cut(s) 482
SaqAI TTAA 2 cut(s) 6, 513
SatI GCNGC 1 cut(s) 229
Sau3AI GATC 3 cut(s) 162, 175, 341
Sau96I GGNCC 3 cut(s) 301, 417, 462
SchI GAGTC 2 cut(s) 83, 397
SduI GDGCHC 1 cut(s) 369
SetI ASST 8 cut(s) 53, 126, 318, 390, 437, 450, 486, 491
Sfr274I CTCGAG 1 cut(s) 91
SfuI TTCGAA 1 cut(s) 79
SinI GGWCC 3 cut(s) 301, 417, 462
SlaI CTCGAG 1 cut(s) 91
SmiMI CAYNNNNRTG 1 cut(s) 482
SmlI CTYRAG 1 cut(s) 91
SmoI CTYRAG 1 cut(s) 91
Sse9I AATT 2 cut(s) 75, 548
SsiI CCGC 3 cut(s) 226, 229, 420
SspI AATATT 1 cut(s) 23
StyI CCWWGG 1 cut(s) 100
TaaI ACNGT 2 cut(s) 220, 351
TaiI ACGT 1 cut(s) 486
TaqI TCGA 5 cut(s) 79, 92, 133, 161, 252
TasI AATT 2 cut(s) 75, 548
TatI WGTACW 1 cut(s) 306
TauI GCSGC 1 cut(s) 231
TfiI GAWTC 1 cut(s) 16
Tru1I TTAA 2 cut(s) 6, 513
Tru9I TTAA 2 cut(s) 6, 513
TscAI CASTG 2 cut(s) 223, 354
TseFI GTSAC 1 cut(s) 220
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 1 cut(s) 69
TspRI CASTG 2 cut(s) 223, 354
VpaK11BI GGWCC 3 cut(s) 301, 417, 462
XapI RAATTY 1 cut(s) 75
XceI RCATGY 1 cut(s) 36
XhoI CTCGAG 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.