Rh5DG136500

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0019741
Physical Location & Seq
Forward (+)
13676307 .. 13679617
3311 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG136500.1

Sequence Viewer

Length: 678 bp
ATGAACAGCTATGAAACCAAAGAAAGACTAAGAATTCGAATAACGGACTCCAATAACCAAAGATGGGAAATCCCACAAGACCTTCTCCCACGCCAGACTCCACAAACATCCCACCATCCCATTCCTGAAAGCGACCTCATTTTCACACTCCACAACACCACCCCATTCGGCTTCACCGTCACCCGCCGTTCCTCCAACGACGTCGTATTCGACACCACCCCAAACCCCTCCAACCCTTCCTCCATCCTCGTGTTCAAGGACCAGCACATCCAGCTTTCCTCCTCTCTCCCGGAAACCCGGTCCTCCTTGTACGGCCTCGGTGAACACACCAAACCCTCCTTCAAGCTCCAACCCAATCAGACCCTGACACTCTGGACTGCCGACATTGCCAGCGCCAACCTCGACCTCAACCTCTATGGGTCCCACCCGTTTCACATGGACGTGAGGTCACCGTCCGGTGACGGAAAAGTTACCGCGGGAACCACTCATGGAGTGTTGCTGTTGAATAGTAATGGCATGGATGTAAATTACGAAGGTGACAGGGTTACTTATAAGGTGATTGGTGGAGTTGTGGACTTGTATTTCTTCTCGGGTCCGACGCCCGAATTGGTGATGGAGCAGTACACGGAGCTCATTGGCCGACCTGCCCCCATGCCCTACTGGTCTTTTGGTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00500 ko01100 map00052 map00500 map01100
Pfam Domains
Protein Families
Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

225

Amino Acids

25.25

Weight (kDa)

5.87

Isoelectric Point (pI)

42.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gal_mutarotas_2 PF13802 11 - 170 5.8e-10 Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019741)

Species Orthologous Gene IDs
malus_domestica MD10G1240300.v1.1
rosa_chinensis RchiOBHm_Chr5g0018621 RchiOBHm_Chr5g0020521
rosa_multiflora Rmu_co8282331.1_g000001 Rmu_co8283033.1_g000001 Rmu_sc0007192.1_g000001
rosa_samantha Rh5DG136500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 552
AatII GACGTC 1 cut(s) 204
Acc36I ACCTGC 1 cut(s) 652
AccII CGCG 1 cut(s) 476
AciI CCGC 3 cut(s) 184, 474, 476
AcoI YGGCCR 1 cut(s) 637
AcsI RAATTY 1 cut(s) 33
AcyI GRCGYC 2 cut(s) 201, 599
AfaI GTAC 2 cut(s) 311, 623
AfiI CCNNNNNNNGG 1 cut(s) 64
AgsI TTSAA 3 cut(s) 256, 343, 505
AhdI GACNNNNNGTC 1 cut(s) 445
AjiI CACGTC 1 cut(s) 442
AjuI GAANNNNNNNTTGG 2 cut(s) 323, 355
AluBI AGCT 4 cut(s) 9, 274, 346, 631
AluI AGCT 4 cut(s) 9, 274, 346, 631
Alw21I GWGCWC 1 cut(s) 633
AlwNI CAGNNNCTG 1 cut(s) 364
Ama87I CYCGRG 1 cut(s) 589
AoxI GGCC 2 cut(s) 313, 637
ApoI RAATTY 1 cut(s) 33
ArsI GACNNNNNNTTYG 4 cut(s) 18, 50, 191, 223
AspLEI GCGC 1 cut(s) 395
AspS9I GGNCC 4 cut(s) 259, 300, 420, 593
AsuC2I CCSGG 2 cut(s) 290, 298
AsuHPI GGTGA 8 cut(s) 166, 172, 332, 441, 470, 548, 568, 622
AsuII TTCGAA 1 cut(s) 37
AvaI CYCGRG 1 cut(s) 589
AvaII GGWCC 4 cut(s) 259, 300, 420, 593
BanII GRGCYC 1 cut(s) 633
BauI CACGAG 1 cut(s) 248
Bbv12I GWGCWC 1 cut(s) 633
BccI CCATC 4 cut(s) 57, 123, 251, 607
BceAI ACGGC 2 cut(s) 171, 328
BcnI CCSGG 2 cut(s) 290, 298
BfoI RGCGCY 1 cut(s) 396
BfuAI ACCTGC 1 cut(s) 652
Bme1390I CCNGG 2 cut(s) 290, 298
Bme18I GGWCC 4 cut(s) 259, 300, 420, 593
BmeRI GACNNNNNGTC 1 cut(s) 445
BmeT110I CYCGRG 1 cut(s) 589
BmgBI CACGTC 1 cut(s) 442
BmgT120I GGNCC 4 cut(s) 259, 300, 420, 593
BmiI GGNNCC 4 cut(s) 421, 422, 481, 594
BmrFI CCNGG 2 cut(s) 290, 298
Bpu14I TTCGAA 1 cut(s) 37
BpuMI CCSGG 2 cut(s) 290, 298
BsaHI GRCGYC 2 cut(s) 201, 599
BsaJI CCNNGG 2 cut(s) 316, 474
BsaWI WCCGGW 1 cut(s) 455
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bsc4I CCNNNNNNNGG 1 cut(s) 64
Bse1I ACTGG 1 cut(s) 665
Bse3DI GCAATG 1 cut(s) 384
BseDI CCNNGG 2 cut(s) 316, 474
BseGI GGATG 5 cut(s) 107, 115, 243, 267, 526
BseLI CCNNNNNNNGG 1 cut(s) 64
BseMI GCAATG 1 cut(s) 384
BseNI ACTGG 1 cut(s) 665
BseRI GAGGAG 1 cut(s) 271
Bsh1236I CGCG 1 cut(s) 476
BshFI GGCC 2 cut(s) 315, 639
BsiHKAI GWGCWC 1 cut(s) 633
BsiHKCI CYCGRG 1 cut(s) 589
BsiSI CCGG 3 cut(s) 290, 298, 456
BslFI GGGAC 1 cut(s) 406
BslI CCNNNNNNNGG 1 cut(s) 64
BsmFI GGGAC 1 cut(s) 406
BsnI GGCC 2 cut(s) 315, 639
BsoBI CYCGRG 1 cut(s) 589
Bsp119I TTCGAA 1 cut(s) 37
Bsp1286I GDGCHC 1 cut(s) 633
BspACI CCGC 3 cut(s) 184, 474, 476
BspANI GGCC 2 cut(s) 315, 639
BspFNI CGCG 1 cut(s) 476
BspLI GGNNCC 4 cut(s) 421, 422, 481, 594
BspMI ACCTGC 1 cut(s) 652
BspT104I TTCGAA 1 cut(s) 37
BsrDI GCAATG 1 cut(s) 384
BsrI ACTGG 1 cut(s) 665
BssECI CCNNGG 2 cut(s) 316, 474
BssNI GRCGYC 2 cut(s) 201, 599
BssSI CACGAG 1 cut(s) 248
Bst2BI CACGAG 1 cut(s) 248
Bst4CI ACNGT 2 cut(s) 178, 453
BstACI GRCGYC 2 cut(s) 201, 599
BstBI TTCGAA 1 cut(s) 37
BstC8I GCNNGC 1 cut(s) 391
BstDEI CTNAG 1 cut(s) 29
BstDSI CCRYGG 1 cut(s) 474
BstEII GGTNACC 1 cut(s) 447
BstF5I GGATG 5 cut(s) 107, 115, 243, 267, 526
BstFNI CGCG 1 cut(s) 476
BstH2I RGCGCY 1 cut(s) 396
BstHHI GCGC 1 cut(s) 395
BstMWI GCNNNNNNNGC 2 cut(s) 271, 386
BstPI GGTNACC 1 cut(s) 447
BstSCI CCNGG 2 cut(s) 288, 296
BstUI CGCG 1 cut(s) 476
BsuRI GGCC 2 cut(s) 315, 639
BtgI CCRYGG 1 cut(s) 474
BtrI CACGTC 1 cut(s) 442
BtsCI GGATG 5 cut(s) 107, 115, 243, 267, 526
BveI ACCTGC 1 cut(s) 652
Cac8I GCNNGC 1 cut(s) 391
CaiI CAGNNNCTG 1 cut(s) 364
CfoI GCGC 1 cut(s) 395
Cfr13I GGNCC 4 cut(s) 259, 300, 420, 593
Cfr42I CCGCGG 1 cut(s) 477
CseI GACGC 1 cut(s) 607
Csp6I GTAC 2 cut(s) 310, 622
CviAII CATG 4 cut(s) 436, 488, 517, 652
CviJI RGCY 7 cut(s) 9, 171, 274, 315, 346, 631, 639
CviKI_1 RGCY 7 cut(s) 9, 171, 274, 315, 346, 631, 639
CviQI GTAC 2 cut(s) 310, 622
DdeI CTNAG 1 cut(s) 29
DriI GACNNNNNGTC 1 cut(s) 445
EaeI YGGCCR 1 cut(s) 637
Eam1105I GACNNNNNGTC 1 cut(s) 445
Ecl136II GAGCTC 1 cut(s) 631
Eco24I GRGCYC 1 cut(s) 633
Eco47I GGWCC 4 cut(s) 259, 300, 420, 593
Eco53kI GAGCTC 1 cut(s) 631
Eco88I CYCGRG 1 cut(s) 589
Eco91I GGTNACC 1 cut(s) 447
EcoICRI GAGCTC 1 cut(s) 631
EcoO109I RGGNCCY 1 cut(s) 420
EcoO65I GGTNACC 1 cut(s) 447
EcoRI GAATTC 1 cut(s) 33
EcoT38I GRGCYC 1 cut(s) 633
FaeI CATG 4 cut(s) 439, 491, 520, 655
FaiI YATR 7 cut(s) 12, 417, 437, 489, 518, 552, 653
FaqI GGGAC 1 cut(s) 406
FatI CATG 4 cut(s) 435, 487, 516, 651
FauI CCCGC 2 cut(s) 191, 469
FokI GGATG 5 cut(s) 94, 102, 230, 254, 533
FriOI GRGCYC 1 cut(s) 633
GlaI GCGC 1 cut(s) 394
HaeII RGCGCY 1 cut(s) 396
HaeIII GGCC 2 cut(s) 315, 639
HapII CCGG 3 cut(s) 290, 298, 456
HgaI GACGC 1 cut(s) 607
HhaI GCGC 1 cut(s) 395
Hin1I GRCGYC 2 cut(s) 201, 599
Hin1II CATG 4 cut(s) 439, 491, 520, 655
Hin6I GCGC 1 cut(s) 393
HinP1I GCGC 1 cut(s) 393
HinfI GANTC 2 cut(s) 47, 97
HpaII CCGG 3 cut(s) 290, 298, 456
HphI GGTGA 8 cut(s) 166, 172, 332, 441, 470, 548, 568, 622
Hpy166II GTNNAC 3 cut(s) 323, 574, 624
Hpy188I TCNGA 2 cut(s) 360, 597
Hpy188III TCNNGA 2 cut(s) 125, 373
Hpy8I GTNNAC 3 cut(s) 323, 574, 624
Hpy99I CGWCG 3 cut(s) 203, 206, 601
HpyAV CCTTC 4 cut(s) 92, 246, 349, 527
HpyCH4III ACNGT 2 cut(s) 178, 453
HpyCH4IV ACGT 2 cut(s) 201, 441
HpyF10VI GCNNNNNNNGC 2 cut(s) 271, 386
HpyF3I CTNAG 1 cut(s) 29
HpySE526I ACGT 2 cut(s) 201, 441
Hsp92I GRCGYC 2 cut(s) 201, 599
Hsp92II CATG 4 cut(s) 439, 491, 520, 655
HspAI GCGC 1 cut(s) 393
KflI GGGWCCC 1 cut(s) 420
KspI CCGCGG 1 cut(s) 477
LmnI GCTCC 3 cut(s) 351, 616, 628
MaeII ACGT 2 cut(s) 201, 441
MaeIII GTNAC 6 cut(s) 178, 447, 458, 469, 536, 544
MboII GAAGA 1 cut(s) 577
MhlI GDGCHC 1 cut(s) 633
MluCI AATT 3 cut(s) 33, 526, 605
MlyI GAGTC 2 cut(s) 41, 91
MmeI TCCRAC 4 cut(s) 219, 255, 373, 620
MslI CAYNNNNRTG 2 cut(s) 248, 440
MspA1I CMGCKG 1 cut(s) 476
MspI CCGG 3 cut(s) 290, 298, 456
MspR9I CCNGG 2 cut(s) 290, 298
MvnI CGCG 1 cut(s) 476
MwoI GCNNNNNNNGC 2 cut(s) 271, 386
NciI CCSGG 2 cut(s) 290, 298
NlaIII CATG 4 cut(s) 439, 491, 520, 655
NlaIV GGNNCC 4 cut(s) 421, 422, 481, 594
NmuCI GTSAC 4 cut(s) 178, 447, 458, 536
NspV TTCGAA 1 cut(s) 37
PcsI WCGNNNNNNNCGW 2 cut(s) 174, 207
PfoI TCCNGGA 1 cut(s) 288
PleI GAGTC 2 cut(s) 41, 91
PpsI GAGTC 2 cut(s) 41, 91
PpuMI RGGWCCY 1 cut(s) 420
PsiI TTATAA 1 cut(s) 552
Psp124BI GAGCTC 1 cut(s) 633
Psp5II RGGWCCY 1 cut(s) 420
PspEI GGTNACC 1 cut(s) 447
PspN4I GGNNCC 4 cut(s) 421, 422, 481, 594
PspPI GGNCC 4 cut(s) 259, 300, 420, 593
PspPPI RGGWCCY 1 cut(s) 420
PstNI CAGNNNCTG 1 cut(s) 364
RsaI GTAC 2 cut(s) 311, 623
RsaNI GTAC 2 cut(s) 310, 622
RseI CAYNNNNRTG 2 cut(s) 248, 440
SacI GAGCTC 1 cut(s) 633
SacII CCGCGG 1 cut(s) 477
Sau96I GGNCC 4 cut(s) 259, 300, 420, 593
SchI GAGTC 2 cut(s) 41, 91
ScrFI CCNGG 2 cut(s) 290, 298
SduI GDGCHC 1 cut(s) 633
Sfr303I CCGCGG 1 cut(s) 477
SfuI TTCGAA 1 cut(s) 37
SgrBI CCGCGG 1 cut(s) 477
SinI GGWCC 4 cut(s) 259, 300, 420, 593
SmiMI CAYNNNNRTG 2 cut(s) 248, 440
Sse9I AATT 3 cut(s) 33, 526, 605
SsiI CCGC 3 cut(s) 184, 474, 476
SstI GAGCTC 1 cut(s) 633
StyD4I CCNGG 2 cut(s) 288, 296
TaaI ACNGT 2 cut(s) 178, 453
TaiI ACGT 2 cut(s) 204, 444
TaqI TCGA 3 cut(s) 37, 210, 402
TasI AATT 3 cut(s) 33, 526, 605
TatI WGTACW 1 cut(s) 621
TseFI GTSAC 4 cut(s) 178, 447, 458, 536
Tsp45I GTSAC 4 cut(s) 178, 447, 458, 536
TspDTI ATGAA 2 cut(s) 17, 27
TspGWI ACGGA 3 cut(s) 59, 477, 641
VpaK11BI GGWCC 4 cut(s) 259, 300, 420, 593
XapI RAATTY 1 cut(s) 33
ZraI GACGTC 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.