MD10G1247400.v1.1

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0019167
Physical Location & Seq
Reverse (-)
34047939 .. 34048567
629 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1247400.v1.1.491

Sequence Viewer

Length: 273 bp
ATGTTAAAAAGGCCAGGAAATATTCCACGTGCTGATGGCCAATTAGATATGACAAGGGCATTTGGGGATCGAAAACTAAAGGAGCACATAACTTCAGAACCAGATATAACAGTAAAGAAGGTTGACGCTGAAACAGATTTCATCATTTTGGCAAGTGATGGATTGTGGAAAGTGATGTCAAACCAAGAGGCTTACGATTGCATTGAAGAGTTGGATGATGCTCAAGAAGCAGCTGAGGAGTTGATTAAAGAAGCCTTATCTAGGAAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C RVT_3

Protein Analysis

91

Amino Acids

10.15

Weight (kDa)

4.64

Isoelectric Point (pI)

45.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 10 - 90 2.6e-24 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019167)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34740 AT2G34740 AT2G34740
fragaria_vesca FvH4_3g10800
malus_domestica MD10G1247400.v1.1
prunus_persica Prupe.4G095900_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0261331
rosa_wichuraiana Rw5G011290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 75
AcoI YGGCCR 1 cut(s) 37
AcuI CTGAAG 1 cut(s) 78
AcvI CACGTG 1 cut(s) 29
AfiI CCNNNNNNNGG 1 cut(s) 261
AgsI TTSAA 1 cut(s) 206
AjnI CCWGG 1 cut(s) 13
AluBI AGCT 2 cut(s) 233, 270
AluI AGCT 2 cut(s) 233, 270
Alw21I GWGCWC 1 cut(s) 87
AlwI GGATC 1 cut(s) 75
AoxI GGCC 2 cut(s) 11, 37
ApeKI GCWGC 1 cut(s) 230
BalI TGGCCA 1 cut(s) 39
BbrPI CACGTG 1 cut(s) 29
Bbv12I GWGCWC 1 cut(s) 87
BbvCI CCTCAGC 1 cut(s) 234
BbvI GCAGC 1 cut(s) 242
BccI CCATC 2 cut(s) 29, 152
BciT130I CCWGG 1 cut(s) 15
BfaI CTAG 1 cut(s) 261
BisI GCNGC 1 cut(s) 231
BlsI GCNGC 1 cut(s) 232
Bme1390I CCNGG 1 cut(s) 15
BmrFI CCNGG 1 cut(s) 15
BmsI GCATC 1 cut(s) 208
Bpu10I CCTNAGC 1 cut(s) 234
BpuEI CTTGAG 1 cut(s) 207
BsaAI YACGTR 1 cut(s) 29
Bsc4I CCNNNNNNNGG 1 cut(s) 261
BseBI CCWGG 1 cut(s) 15
BseGI GGATG 1 cut(s) 220
BseLI CCNNNNNNNGG 1 cut(s) 261
BseMII CTCAG 1 cut(s) 225
BseRI GAGGAG 1 cut(s) 251
BseXI GCAGC 1 cut(s) 242
BshFI GGCC 2 cut(s) 13, 39
BsiHKAI GWGCWC 1 cut(s) 87
BslI CCNNNNNNNGG 1 cut(s) 261
BsnI GGCC 2 cut(s) 13, 39
Bsp1286I GDGCHC 1 cut(s) 87
Bsp143I GATC 1 cut(s) 67
BspANI GGCC 2 cut(s) 13, 39
BspCNI CTCAG 1 cut(s) 226
BspPI GGATC 1 cut(s) 75
BssMI GATC 1 cut(s) 67
Bst2UI CCWGG 1 cut(s) 15
Bst4CI ACNGT 1 cut(s) 112
Bst6I CTCTTC 1 cut(s) 201
BstBAI YACGTR 1 cut(s) 29
BstDEI CTNAG 1 cut(s) 234
BstENI CCTNNNNNAGG 1 cut(s) 259
BstF5I GGATG 1 cut(s) 220
BstKTI GATC 1 cut(s) 70
BstMBI GATC 1 cut(s) 67
BstMWI GCNNNNNNNGC 1 cut(s) 227
BstNI CCWGG 1 cut(s) 15
BstSCI CCNGG 1 cut(s) 13
BstV1I GCAGC 1 cut(s) 242
BsuRI GGCC 2 cut(s) 13, 39
BtsCI GGATG 1 cut(s) 220
CseI GACGC 1 cut(s) 134
CviJI RGCY 6 cut(s) 13, 39, 191, 233, 254, 270
CviKI_1 RGCY 6 cut(s) 13, 39, 191, 233, 254, 270
DdeI CTNAG 1 cut(s) 234
DpnI GATC 1 cut(s) 69
DpnII GATC 1 cut(s) 67
EaeI YGGCCR 1 cut(s) 37
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
Eco57I CTGAAG 1 cut(s) 78
Eco72I CACGTG 1 cut(s) 29
EcoNI CCTNNNNNAGG 1 cut(s) 259
EcoRII CCWGG 1 cut(s) 13
FaiI YATR 3 cut(s) 50, 89, 107
Fnu4HI GCNGC 1 cut(s) 231
FokI GGATG 1 cut(s) 227
Fsp4HI GCNGC 1 cut(s) 231
FspBI CTAG 1 cut(s) 261
GluI GCNGC 1 cut(s) 231
HaeIII GGCC 2 cut(s) 13, 39
HgaI GACGC 1 cut(s) 134
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 1 cut(s) 97
Hpy188III TCNNGA 1 cut(s) 224
Hpy8I GTNNAC 1 cut(s) 124
HpyAV CCTTC 1 cut(s) 112
HpyCH4III ACNGT 1 cut(s) 112
HpyCH4IV ACGT 1 cut(s) 28
HpyCH4V TGCA 1 cut(s) 201
HpyF10VI GCNNNNNNNGC 1 cut(s) 227
HpyF3I CTNAG 1 cut(s) 234
HpySE526I ACGT 1 cut(s) 28
Kzo9I GATC 1 cut(s) 67
LmnI GCTCC 1 cut(s) 82
LpnPI CCDG 2 cut(s) 27, 114
Lsp1109I GCAGC 1 cut(s) 242
LweI GCATC 1 cut(s) 208
MaeI CTAG 1 cut(s) 261
MaeII ACGT 1 cut(s) 28
MalI GATC 1 cut(s) 69
MboI GATC 1 cut(s) 67
MboII GAAGA 1 cut(s) 218
MhlI GDGCHC 1 cut(s) 87
MlsI TGGCCA 1 cut(s) 39
MluCI AATT 1 cut(s) 41
MluNI TGGCCA 1 cut(s) 39
MmeI TCCRAC 1 cut(s) 192
MnlI CCTC 2 cut(s) 181, 229
Mox20I TGGCCA 1 cut(s) 39
MscI TGGCCA 1 cut(s) 39
MseI TTAA 2 cut(s) 5, 246
Msp20I TGGCCA 1 cut(s) 39
MspA1I CMGCKG 1 cut(s) 233
MspR9I CCNGG 1 cut(s) 15
MvaI CCWGG 1 cut(s) 15
MwoI GCNNNNNNNGC 1 cut(s) 227
NdeII GATC 1 cut(s) 67
PkrI GCNGC 1 cut(s) 232
PmaCI CACGTG 1 cut(s) 29
PmlI CACGTG 1 cut(s) 29
Ppu21I YACGTR 1 cut(s) 29
Psp6I CCWGG 1 cut(s) 13
PspCI CACGTG 1 cut(s) 29
PspGI CCWGG 1 cut(s) 13
PvuII CAGCTG 1 cut(s) 233
SaqAI TTAA 2 cut(s) 5, 246
SatI GCNGC 1 cut(s) 231
Sau3AI GATC 1 cut(s) 67
ScrFI CCNGG 1 cut(s) 15
SduI GDGCHC 1 cut(s) 87
SetI ASST 4 cut(s) 31, 123, 235, 272
SfaNI GCATC 1 cut(s) 208
SgeI CNNG 9 cut(s) 26, 27, 39, 41, 66, 113, 165, 197, 236
SmlI CTYRAG 1 cut(s) 222
SmoI CTYRAG 1 cut(s) 222
Sse9I AATT 1 cut(s) 41
SspI AATATT 1 cut(s) 22
SspMI CTAG 1 cut(s) 261
StyD4I CCNGG 1 cut(s) 13
TaaI ACNGT 1 cut(s) 112
TaiI ACGT 1 cut(s) 31
TaqI TCGA 1 cut(s) 70
TasI AATT 1 cut(s) 41
Tru1I TTAA 2 cut(s) 5, 246
Tru9I TTAA 2 cut(s) 5, 246
TseI GCWGC 1 cut(s) 230
TspDTI ATGAA 1 cut(s) 130
XagI CCTNNNNNAGG 1 cut(s) 259
XspI CTAG 1 cut(s) 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.