MD10G1259000.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0016778
Physical Location & Seq
Forward (+)
35322119 .. 35324251
2133 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1259000.v1.1.491

Sequence Viewer

Length: 2112 bp
ATGTTTCAACGGTCACCACATATTCTCCACAGCCCTCTCCTCAAATTCCTTCACTTTCCCGCTACCCAAACAACCCCTTTCAGCACGCTCTCCTCCCTCTTCAGCCTCTGCACCGAACCCCGAAAGCTCAAGCAAATCCACGCCGGGTTCGTCCTTCACGGCCAGCACCAAAACCCAACACTCTCTTCCCAACTCATCGACCGCTATGCCAACCTCGGCCTCCTCGGTCGTTCCGAACAGGTCTTCAACTCTGTCACCGACCCAAGTCCGATTCTTTACGACGCAATCATTAGAAAGCTGTCGGAGTTTGGTCAGTTCGAGAAAACCCTTTTGGTTTACAATGATATGGTCATGAAATCAATTTACCCAGATGGAAATACTTACCCTTTTGTTTTGAAATCGTGTTCGGATGCTAGAATTGGGGGAAAGATTCATGGGCATGTGGTGAAATTGGGTTTTGATTCGTATGATTTGGTGGGTGCTGCTTTGGTTGATATGTATGGAAATTATGGGGGATTTGGGAATGCAGGAGAGGTGGTTGATGAAATGTCTGTTTGGGAGGGTGGTTTGGATTTTTCGATTTCTGCGGCTTTGCGAAGCGGGAAAGCAATGGAGAAACTTGAGCCTGAATCGGTTAATTCAGTTACTGTGATTAACTTGCTGAGATCAAGTGTGGATTTGAACTCACTGAAGGCTGGAAAGGCTGTTCATTGCGTGGTGGTTGTGAGAAATTTGTGTGAGGATTTGTCTGTGAATACTGCATTGTTGTCTATGTATGCCAAGTTGGGTGATTTGAAATATGCAGAATTGTTGTTTGGAAAAATGCCTGACAAGGACTCAGTTGTTTGGAACATAATGATCTCAGCTTATGCTCGAAATCGGCATCCTAAGGAATCACTTGAGCTATTGAGATGCATGGGAAGATCAGGGGTTAGAGCTGATTTATTCACTGCAATCCCTGCAATTTCGTCAATTGCGCAGTTAAGAGCTGCTGATTGGGGCAAGCAAATGCATGCTCATGTGATAAGAAATGGTTCAGATTATCAAGTTTCTATTCACAATTCTCTTATTCACATGTACTGTGCTTGTGATGATCTGAATTCGGCACAAAAGATTTTCGACTTGGTGCCTAACAAGACTGTGGTTTCATGGAGTGCAATGATCAAAGGATATGTATCCCATGATCAGAGTCTTGACGCGCTTTCTCTCTTCGCCAAGATGAAAGTTGATGGATTAGTTGTCGATTTCATTACAGTAATTAACATCTTGCCTGCTTGTGTGAACCTTGGGGCACTAGAGAATGTAAAGTACCTTCATGGGTACTCAATGAAACTCAGCCTCAACTCGCTTTCATCTGTTAATACGGCGTTTCTAGTCAGCTATGCGAAATGTGGATGCATAGAGATGGCCCGGAAGCTTTTTGACGAAGAAAACATCAACACTAAAGATGTAATCACGTGGAATTCTATGATTAGCGCGTATGCTAAGCATGGAGACTGGTCTCGTTCTTTCGAGTTGTACCACCAAATGAAGAACTCGAGCTTGAAGCCAGATCAAGTAACCTTTCTCGGAGTACTGACAGCCTGTGTAAACGCAGGTCTTGTTCAAGAAGGAAAGGAACGTTTCAAGGAGATGGTTGAAAAATACGATTGCCAACCAAGCCATGAACACTATGCTTGCATGGTGGATCTGCTAGGGCGTGCCGGGCATGTCAACGAAGCCAGAGAGCTAGTAAAATCTATGCCTTTCAGACCGGATGCTCGGGTTTGGGGGCCTTTGTTGAGCGCTTGCAAGTTGCATTCGGAGACTGGAGTTGCAGAGTTCGCTGCTGAGAAGCTTATAACCATGGAACCGAAAAATCCCGGAAATTATATATTGCTTGCCAACATATATGCTGCAGCTGGAAAGTGGGATGGAGTTGCTAAGATGAGGAGTTCCCTCAGAAACATAGGCCTGAAGAAAACTCCGGGACTGAGCTGGGTAGAGATAAACGGACGTCAACATGAATTCCGAGTAGCGGACCGTTCTCATCCGAGATCCGACGGTATATATGCCACATTAAGAAACCTGGAGTGGGAAATCAAGGATGCAAGAGACAGAGATAGTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

704

Amino Acids

78.18

Weight (kDa)

8.07

Isoelectric Point (pI)

44.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 278 - 317 3.1e-06 PPR repeat family
PPR PF01535 281 - 311 4.8e-07 PPR repeat
PPR_2 PF13041 380 - 426 7.7e-06 PPR repeat family
PPR PF01535 382 - 412 6.7e-06 PPR repeat
PPR_2 PF13041 482 - 530 3.6e-13 PPR repeat family
PPR PF01535 485 - 513 2.1e-08 PPR repeat
PPR_long PF17177 491 - 636 9.9e-07 Pentacotripeptide-repeat region of PRORP
E_motif PF20431 600 - 662 1.8e-21 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016778)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g05170
malus_domestica MD10G1259000.v1.1
prunus_persica Prupe.4G084300_v2.0.a1
pyrus_communis pycom10g21550
rosa_chinensis RchiOBHm_Chr5g0014581
rosa_laevigata RLG00000032130
rosa_multiflora Rmu_co8518221.1_g000001 Rmu_sc0000120.1_g000005
rosa_roxburghii Rroxscaffold_1G00061900
rosa_rugosa Rorug05G0019600
rosa_samantha Rh5AG114800 Rh5BG111700 Rh5CG123200
rosa_wichuraiana Rw5G009940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1841
AatII GACGTC 1 cut(s) 1999
Acc16I TGCGCA 1 cut(s) 978
Acc36I ACCTGC 1 cut(s) 1586
AccB1I GGYRCC 1 cut(s) 1126
AccII CGCG 2 cut(s) 1199, 1478
AciI CCGC 5 cut(s) 60, 202, 587, 600, 2018
AclI AACGTT 1 cut(s) 1621
AclWI GGATC 2 cut(s) 1695, 2031
AcoI YGGCCR 1 cut(s) 160
AcsI RAATTY 5 cut(s) 44, 730, 1099, 1462, 2006
AcuI CTGAAG 3 cut(s) 85, 710, 1976
AcvI CACGTG 1 cut(s) 1458
AcyI GRCGYC 1 cut(s) 1996
AfaI GTAC 5 cut(s) 1079, 1310, 1322, 1520, 1575
AfeI AGCGCT 1 cut(s) 1786
AfiI CCNNNNNNNGG 2 cut(s) 2017, 2074
AflIII ACRYGT 1 cut(s) 1074
AgsI TTSAA 9 cut(s) 8, 247, 397, 682, 796, 1546, 1607, 1627, 1640
AjnI CCWGG 1 cut(s) 2067
AjuI GAANNNNNNNTTGG 6 cut(s) 169, 201, 314, 346, 798, 830
AloI GAACNNNNNNTCC 2 cut(s) 690, 722
Alw26I GTCTC 4 cut(s) 1488, 1506, 1799, 2088
AlwI GGATC 2 cut(s) 1695, 2031
AlwNI CAGNNNCTG 2 cut(s) 108, 647
Ama87I CYCGRG 2 cut(s) 1537, 1761
Aor51HI AGCGCT 1 cut(s) 1786
AoxI GGCC 5 cut(s) 160, 217, 1407, 1772, 1951
ApeKI GCWGC 5 cut(s) 482, 989, 1826, 1895, 1898
ApoI RAATTY 5 cut(s) 44, 730, 1099, 1462, 2006
Asp700I GAANNNNTTC 1 cut(s) 1033
AspLEI GCGC 4 cut(s) 979, 1201, 1478, 1787
AspS9I GGNCC 3 cut(s) 1408, 1772, 2020
AsuC2I CCSGG 5 cut(s) 145, 1411, 1705, 1863, 1968
AsuHPI GGTGA 4 cut(s) 6, 247, 457, 800
AvaI CYCGRG 2 cut(s) 1537, 1761
AvaII GGWCC 1 cut(s) 2020
AxyI CCTNAGG 1 cut(s) 888
BaeGI GKGCMC 1 cut(s) 1294
BanI GGYRCC 1 cut(s) 1126
BarI GAAGNNNNNNTAC 2 cut(s) 1296, 1328
BbrPI CACGTG 1 cut(s) 1458
BbsI GAAGAC 1 cut(s) 235
BbvI GCAGC 5 cut(s) 469, 976, 1813, 1882, 1910
BccI CCATC 5 cut(s) 365, 1223, 1399, 1627, 1907
BceAI ACGGC 2 cut(s) 175, 1380
BcgI CGANNNNNNTGC 2 cut(s) 188, 222
BciT130I CCWGG 1 cut(s) 2069
BciVI GTATCC 1 cut(s) 1186
BclI TGATCA 2 cut(s) 1161, 1183
BcnI CCSGG 5 cut(s) 145, 1411, 1705, 1863, 1968
BcoDI GTCTC 4 cut(s) 1488, 1506, 1799, 2088
BfaI CTAG 5 cut(s) 414, 1295, 1373, 1694, 1730
BfmI CTRYAG 1 cut(s) 1896
BfoI RGCGCY 1 cut(s) 1788
BfuAI ACCTGC 1 cut(s) 1586
BfuI GTATCC 1 cut(s) 1186
BisI GCNGC 6 cut(s) 483, 588, 990, 1827, 1896, 1899
BlpI GCTNAGC 1 cut(s) 1485
BlsI GCNGC 6 cut(s) 484, 589, 991, 1828, 1897, 1900
BmcAI AGTACT 1 cut(s) 1575
Bme1390I CCNGG 6 cut(s) 145, 1411, 1705, 1863, 1968, 2069
Bme18I GGWCC 1 cut(s) 2020
BmeT110I CYCGRG 2 cut(s) 1537, 1761
BmgT120I GGNCC 3 cut(s) 1408, 1772, 2020
BmiI GGNNCC 3 cut(s) 1128, 1773, 1851
BmrFI CCNGG 6 cut(s) 145, 1411, 1705, 1863, 1968, 2069
BmsI GCATC 6 cut(s) 400, 892, 902, 1385, 1747, 2077
BoxI GACNNNNGTC 1 cut(s) 264
BpiI GAAGAC 1 cut(s) 235
BpmI CTGGAG 2 cut(s) 1830, 2090
Bpu1102I GCTNAGC 1 cut(s) 1485
BpuEI CTTGAG 3 cut(s) 113, 641, 920
BpuMI CCSGG 5 cut(s) 145, 1411, 1705, 1863, 1968
BsaAI YACGTR 1 cut(s) 1458
BsaBI GATNNNNATC 1 cut(s) 1174
BsaHI GRCGYC 1 cut(s) 1996
BsaI GGTCTC 1 cut(s) 1506
BsaJI CCNNGG 4 cut(s) 214, 223, 1285, 1845
BsaWI WCCGGW 1 cut(s) 1753
BsaXI ACNNNNNCTCC 4 cut(s) 9, 39, 522, 552
Bsc4I CCNNNNNNNGG 2 cut(s) 2017, 2074
Bse1I ACTGG 2 cut(s) 1502, 1813
Bse21I CCTNAGG 1 cut(s) 888
Bse3DI GCAATG 3 cut(s) 615, 709, 1164
Bse8I GATNNNNATC 1 cut(s) 1174
BseBI CCWGG 1 cut(s) 2069
BseDI CCNNGG 4 cut(s) 214, 223, 1285, 1845
BseGI GGATG 7 cut(s) 415, 883, 1400, 1762, 1918, 2029, 2092
BseJI GATNNNNATC 1 cut(s) 1174
BseLI CCNNNNNNNGG 2 cut(s) 2017, 2074
BseMI GCAATG 3 cut(s) 615, 709, 1164
BseMII CTCAG 7 cut(s) 653, 852, 876, 1348, 1821, 1954, 1964
BseNI ACTGG 2 cut(s) 1502, 1813
BseRI GAGGAG 4 cut(s) 29, 82, 212, 1945
BseSI GKGCMC 1 cut(s) 1294
BseXI GCAGC 5 cut(s) 469, 976, 1813, 1882, 1910
BseYI CCCAGC 1 cut(s) 1977
BsgI GTGCAG 1 cut(s) 94
Bsh1236I CGCG 2 cut(s) 1199, 1478
Bsh1285I CGRYCG 2 cut(s) 202, 229
BshFI GGCC 5 cut(s) 162, 219, 1409, 1774, 1953
BshNI GGYRCC 1 cut(s) 1126
BsiEI CGRYCG 2 cut(s) 202, 229
BsiHKCI CYCGRG 2 cut(s) 1537, 1761
BsiSI CCGG 6 cut(s) 144, 1411, 1704, 1754, 1863, 1967
BslFI GGGAC 1 cut(s) 1983
BslI CCNNNNNNNGG 2 cut(s) 2017, 2074
BsmAI GTCTC 4 cut(s) 1488, 1506, 1799, 2088
BsmFI GGGAC 1 cut(s) 1983
BsmI GAATGC 2 cut(s) 529, 1798
BsnI GGCC 5 cut(s) 162, 219, 1409, 1774, 1953
Bso31I GGTCTC 1 cut(s) 1506
BsoBI CYCGRG 2 cut(s) 1537, 1761
Bsp1286I GDGCHC 1 cut(s) 1294
Bsp143I GATC 9 cut(s) 665, 858, 923, 1093, 1161, 1183, 1552, 1687, 2036
Bsp1720I GCTNAGC 1 cut(s) 1485
Bsp19I CCATGG 1 cut(s) 1845
BspACI CCGC 5 cut(s) 60, 202, 587, 600, 2018
BspANI GGCC 5 cut(s) 162, 219, 1409, 1774, 1953
BspCNI CTCAG 7 cut(s) 654, 851, 875, 1347, 1822, 1953, 1965
BspFNI CGCG 2 cut(s) 1199, 1478
BspHI TCATGA 1 cut(s) 351
BspLI GGNNCC 3 cut(s) 1128, 1773, 1851
BspMAI CTGCAG 1 cut(s) 1900
BspMI ACCTGC 1 cut(s) 1586
BspPI GGATC 2 cut(s) 1695, 2031
BspT107I GGYRCC 1 cut(s) 1126
BspTNI GGTCTC 1 cut(s) 1506
BsrDI GCAATG 3 cut(s) 615, 709, 1164
BsrI ACTGG 2 cut(s) 1502, 1813
BssECI CCNNGG 4 cut(s) 214, 223, 1285, 1845
BssMI GATC 9 cut(s) 665, 858, 923, 1093, 1161, 1183, 1552, 1687, 2036
BssNI GRCGYC 1 cut(s) 1996
BssT1I CCWWGG 2 cut(s) 1285, 1845
Bst2UI CCWGG 1 cut(s) 2069
Bst4CI ACNGT 7 cut(s) 12, 649, 1082, 1141, 1255, 2024, 2045
Bst6I CTCTTC 3 cut(s) 104, 190, 1214
BstACI GRCGYC 1 cut(s) 1996
BstAPI GCANNNNNTGC 1 cut(s) 959
BstBAI YACGTR 1 cut(s) 1458
BstC8I GCNNGC 9 cut(s) 86, 164, 1004, 1014, 1272, 1678, 1701, 1789, 1881
BstDSI CCRYGG 1 cut(s) 1845
BstEII GGTNACC 1 cut(s) 12
BstF5I GGATG 7 cut(s) 415, 883, 1400, 1762, 1918, 2029, 2092
BstFNI CGCG 2 cut(s) 1199, 1478
BstH2I RGCGCY 1 cut(s) 1788
BstHHI GCGC 4 cut(s) 979, 1201, 1478, 1787
BstKTI GATC 9 cut(s) 668, 861, 926, 1096, 1164, 1186, 1555, 1690, 2039
BstMAI GTCTC 4 cut(s) 1488, 1506, 1799, 2088
BstMBI GATC 9 cut(s) 665, 858, 923, 1093, 1161, 1183, 1552, 1687, 2036
BstMCI CGRYCG 2 cut(s) 202, 229
BstMWI GCNNNNNNNGC 5 cut(s) 701, 959, 1659, 1705, 1823
BstNI CCWGG 1 cut(s) 2069
BstNSI RCATGY 4 cut(s) 443, 1016, 1078, 1712
BstPAI GACNNNNGTC 1 cut(s) 264
BstPI GGTNACC 1 cut(s) 12
BstSCI CCNGG 6 cut(s) 143, 1409, 1703, 1861, 1966, 2067
BstSFI CTRYAG 1 cut(s) 1896
BstSLI GKGCMC 1 cut(s) 1294
BstUI CGCG 2 cut(s) 1199, 1478
BstV1I GCAGC 5 cut(s) 469, 976, 1813, 1882, 1910
BstV2I GAAGAC 1 cut(s) 235
BstX2I RGATCY 2 cut(s) 1687, 2036
BstYI RGATCY 2 cut(s) 1687, 2036
Bsu36I CCTNAGG 1 cut(s) 888
BsuI GTATCC 1 cut(s) 1186
BsuRI GGCC 5 cut(s) 162, 219, 1409, 1774, 1953
BtgI CCRYGG 1 cut(s) 1845
BtsCI GGATG 7 cut(s) 415, 883, 1400, 1762, 1918, 2029, 2092
BtsI GCAGTG 1 cut(s) 948
BtsIMutI CAGTG 2 cut(s) 686, 948
BveI ACCTGC 1 cut(s) 1586
Cac8I GCNNGC 9 cut(s) 86, 164, 1004, 1014, 1272, 1678, 1701, 1789, 1881
CaiI CAGNNNCTG 2 cut(s) 108, 647
CciI TCATGA 1 cut(s) 351
CfoI GCGC 4 cut(s) 979, 1201, 1478, 1787
Cfr13I GGNCC 3 cut(s) 1408, 1772, 2020
CpoI CGGWCCG 1 cut(s) 2020
CseI GACGC 2 cut(s) 290, 1205
Csp6I GTAC 5 cut(s) 1078, 1309, 1321, 1519, 1574
CspI CGGWCCG 1 cut(s) 2020
CviQI GTAC 5 cut(s) 1078, 1309, 1321, 1519, 1574
DpnI GATC 9 cut(s) 667, 860, 925, 1095, 1163, 1185, 1554, 1689, 2038
DpnII GATC 9 cut(s) 665, 858, 923, 1093, 1161, 1183, 1552, 1687, 2036
EaeI YGGCCR 1 cut(s) 160
Eam1104I CTCTTC 3 cut(s) 104, 190, 1214
EarI CTCTTC 3 cut(s) 104, 190, 1214
Eco130I CCWWGG 2 cut(s) 1285, 1845
Eco147I AGGCCT 1 cut(s) 1953
Eco31I GGTCTC 1 cut(s) 1506
Eco47I GGWCC 1 cut(s) 2020
Eco47III AGCGCT 1 cut(s) 1786
Eco57I CTGAAG 3 cut(s) 85, 710, 1976
Eco72I CACGTG 1 cut(s) 1458
Eco81I CCTNAGG 1 cut(s) 888
Eco88I CYCGRG 2 cut(s) 1537, 1761
Eco91I GGTNACC 1 cut(s) 12
EcoO109I RGGNCCY 1 cut(s) 1772
EcoO65I GGTNACC 1 cut(s) 12
EcoRI GAATTC 3 cut(s) 1099, 1462, 2006
EcoRII CCWGG 1 cut(s) 2067
EcoT14I CCWWGG 2 cut(s) 1285, 1845
EcoT22I ATGCAT 3 cut(s) 917, 1014, 1400
ErhI CCWWGG 2 cut(s) 1285, 1845
FaqI GGGAC 1 cut(s) 1983
FauI CCCGC 2 cut(s) 67, 593
FbaI TGATCA 2 cut(s) 1161, 1183
Fnu4HI GCNGC 6 cut(s) 483, 588, 990, 1827, 1896, 1899
FokI GGATG 7 cut(s) 422, 870, 1407, 1769, 1925, 2016, 2099
Fsp4HI GCNGC 6 cut(s) 483, 588, 990, 1827, 1896, 1899
FspBI CTAG 5 cut(s) 414, 1295, 1373, 1694, 1730
FspI TGCGCA 1 cut(s) 978
GlaI GCGC 4 cut(s) 978, 1200, 1477, 1786
GluI GCNGC 6 cut(s) 483, 588, 990, 1827, 1896, 1899
GsaI CCCAGC 1 cut(s) 1981
GsuI CTGGAG 2 cut(s) 1830, 2090
HaeII RGCGCY 1 cut(s) 1788
HaeIII GGCC 5 cut(s) 162, 219, 1409, 1774, 1953
HapII CCGG 6 cut(s) 144, 1411, 1704, 1754, 1863, 1967
HgaI GACGC 2 cut(s) 290, 1205
HhaI GCGC 4 cut(s) 979, 1201, 1478, 1787
Hin1I GRCGYC 1 cut(s) 1996
Hin6I GCGC 4 cut(s) 977, 1199, 1476, 1785
HinP1I GCGC 4 cut(s) 977, 1199, 1476, 1785
HincII GTYRAC 2 cut(s) 1714, 2000
HindII GTYRAC 2 cut(s) 1714, 2000
HindIII AAGCTT 2 cut(s) 1415, 1835
HinfI GANTC 7 cut(s) 271, 430, 461, 629, 836, 893, 1189
HpaII CCGG 6 cut(s) 144, 1411, 1704, 1754, 1863, 1967
HphI GGTGA 4 cut(s) 6, 247, 457, 800
Hpy166II GTNNAC 5 cut(s) 337, 1282, 1591, 1714, 2000
Hpy188III TCNNGA 4 cut(s) 319, 352, 1193, 1607
Hpy8I GTNNAC 5 cut(s) 337, 1282, 1591, 1714, 2000
Hpy99I CGWCG 2 cut(s) 284, 2045
HpyAV CCTTC 5 cut(s) 59, 164, 685, 1322, 1604
HpyCH4III ACNGT 7 cut(s) 12, 649, 1082, 1141, 1255, 2024, 2045
HpyCH4IV ACGT 3 cut(s) 1457, 1621, 1996
HpyF10VI GCNNNNNNNGC 5 cut(s) 701, 959, 1659, 1705, 1823
HpySE526I ACGT 3 cut(s) 1457, 1621, 1996
Hsp92I GRCGYC 1 cut(s) 1996
HspAI GCGC 4 cut(s) 977, 1199, 1476, 1785
Ksp22I TGATCA 2 cut(s) 1161, 1183
Kzo9I GATC 9 cut(s) 665, 858, 923, 1093, 1161, 1183, 1552, 1687, 2036
Lsp1109I GCAGC 5 cut(s) 469, 976, 1813, 1882, 1910
LweI GCATC 6 cut(s) 400, 892, 902, 1385, 1747, 2077
MaeI CTAG 5 cut(s) 414, 1295, 1373, 1694, 1730
MaeII ACGT 3 cut(s) 1457, 1621, 1996
MaeIII GTNAC 4 cut(s) 12, 253, 643, 1558
MalI GATC 9 cut(s) 667, 860, 925, 1095, 1163, 1185, 1554, 1689, 2038
MboI GATC 9 cut(s) 665, 858, 923, 1093, 1161, 1183, 1552, 1687, 2036
MboII GAAGA 8 cut(s) 91, 177, 235, 933, 1201, 1439, 1543, 1969
MfeI CAATTG 1 cut(s) 972
MflI RGATCY 2 cut(s) 1687, 2036
MhlI GDGCHC 1 cut(s) 1294
MlyI GAGTC 2 cut(s) 830, 1198
MmeI TCCRAC 2 cut(s) 282, 2064
Mph1103I ATGCAT 3 cut(s) 917, 1014, 1400
MroXI GAANNNNTTC 1 cut(s) 1033
MseI TTAA 6 cut(s) 636, 654, 983, 1260, 1359, 2060
MslI CAYNNNNRTG 3 cut(s) 438, 1017, 1403
MspA1I CMGCKG 1 cut(s) 1901
MspI CCGG 6 cut(s) 144, 1411, 1704, 1754, 1863, 1967
MspR9I CCNGG 6 cut(s) 145, 1411, 1705, 1863, 1968, 2069
MunI CAATTG 1 cut(s) 972
Mva1269I GAATGC 2 cut(s) 529, 1798
MvaI CCWGG 1 cut(s) 2069
MvnI CGCG 2 cut(s) 1199, 1478
MwoI GCNNNNNNNGC 5 cut(s) 701, 959, 1659, 1705, 1823
NciI CCSGG 5 cut(s) 145, 1411, 1705, 1863, 1968
NcoI CCATGG 1 cut(s) 1845
NdeII GATC 9 cut(s) 665, 858, 923, 1093, 1161, 1183, 1552, 1687, 2036
NlaIV GGNNCC 3 cut(s) 1128, 1773, 1851
NmeAIII GCCGAG 1 cut(s) 195
NmuCI GTSAC 2 cut(s) 12, 253
NsbI TGCGCA 1 cut(s) 978
NsiI ATGCAT 3 cut(s) 917, 1014, 1400
NspI RCATGY 4 cut(s) 443, 1016, 1078, 1712
PaeI GCATGC 1 cut(s) 1016
PaeR7I CTCGAG 1 cut(s) 1537
PagI TCATGA 1 cut(s) 351
PceI AGGCCT 1 cut(s) 1953
PciI ACATGT 1 cut(s) 1074
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 231
PctI GAATGC 2 cut(s) 529, 1798
PdmI GAANNNNTTC 1 cut(s) 1033
PfeI GAWTC 5 cut(s) 271, 430, 461, 629, 893
PfoI TCCNGGA 2 cut(s) 1861, 1966
PkrI GCNGC 6 cut(s) 484, 589, 991, 1828, 1897, 1900
PleI GAGTC 2 cut(s) 830, 1197
PmaCI CACGTG 1 cut(s) 1458
PmlI CACGTG 1 cut(s) 1458
PpsI GAGTC 2 cut(s) 830, 1197
Ppu21I YACGTR 1 cut(s) 1458
PscI ACATGT 1 cut(s) 1074
PshAI GACNNNNGTC 1 cut(s) 264
PsiI TTATAA 1 cut(s) 1841
Psp1406I AACGTT 1 cut(s) 1621
Psp6I CCWGG 1 cut(s) 2067
PspCI CACGTG 1 cut(s) 1458
PspEI GGTNACC 1 cut(s) 12
PspFI CCCAGC 1 cut(s) 1977
PspGI CCWGG 1 cut(s) 2067
PspN4I GGNNCC 3 cut(s) 1128, 1773, 1851
PspPI GGNCC 3 cut(s) 1408, 1772, 2020
PspXI VCTCGAGB 1 cut(s) 1537
PstI CTGCAG 1 cut(s) 1900
PstNI CAGNNNCTG 2 cut(s) 108, 647
PsuI RGATCY 2 cut(s) 1687, 2036
PvuII CAGCTG 1 cut(s) 1901
RsaI GTAC 5 cut(s) 1079, 1310, 1322, 1520, 1575
RsaNI GTAC 5 cut(s) 1078, 1309, 1321, 1519, 1574
RseI CAYNNNNRTG 3 cut(s) 438, 1017, 1403
Rsr2I CGGWCCG 1 cut(s) 2020
RsrII CGGWCCG 1 cut(s) 2020
SaqAI TTAA 6 cut(s) 636, 654, 983, 1260, 1359, 2060
SatI GCNGC 6 cut(s) 483, 588, 990, 1827, 1896, 1899
Sau3AI GATC 9 cut(s) 665, 858, 923, 1093, 1161, 1183, 1552, 1687, 2036
Sau96I GGNCC 3 cut(s) 1408, 1772, 2020
ScaI AGTACT 1 cut(s) 1575
SchI GAGTC 2 cut(s) 830, 1198
ScrFI CCNGG 6 cut(s) 145, 1411, 1705, 1863, 1968, 2069
SduI GDGCHC 1 cut(s) 1294
SfaNI GCATC 6 cut(s) 400, 892, 902, 1385, 1747, 2077
SfcI CTRYAG 1 cut(s) 1896
Sfr274I CTCGAG 1 cut(s) 1537
SinI GGWCC 1 cut(s) 2020
SlaI CTCGAG 1 cut(s) 1537
SmiMI CAYNNNNRTG 3 cut(s) 438, 1017, 1403
SmlI CTYRAG 4 cut(s) 128, 620, 899, 1537
SmoI CTYRAG 4 cut(s) 128, 620, 899, 1537
SphI GCATGC 1 cut(s) 1016
SseBI AGGCCT 1 cut(s) 1953
SsiI CCGC 5 cut(s) 60, 202, 587, 600, 2018
SspMI CTAG 5 cut(s) 414, 1295, 1373, 1694, 1730
StuI AGGCCT 1 cut(s) 1953
StyD4I CCNGG 6 cut(s) 143, 1409, 1703, 1861, 1966, 2067
StyI CCWWGG 2 cut(s) 1285, 1845
TaaI ACNGT 7 cut(s) 12, 649, 1082, 1141, 1255, 2024, 2045
TaiI ACGT 3 cut(s) 1460, 1624, 1999
TaqI TCGA 8 cut(s) 198, 318, 578, 874, 1119, 1242, 1512, 1538
TaqII GACCGA 1 cut(s) 215
TatI WGTACW 2 cut(s) 1077, 1573
TauI GCSGC 1 cut(s) 590
TfiI GAWTC 5 cut(s) 271, 430, 461, 629, 893
Tru1I TTAA 6 cut(s) 636, 654, 983, 1260, 1359, 2060
Tru9I TTAA 6 cut(s) 636, 654, 983, 1260, 1359, 2060
TscAI CASTG 2 cut(s) 693, 955
TseFI GTSAC 2 cut(s) 12, 253
TseI GCWGC 5 cut(s) 482, 989, 1826, 1895, 1898
Tsp45I GTSAC 2 cut(s) 12, 253
TspGWI ACGGA 1 cut(s) 2007
TspRI CASTG 2 cut(s) 693, 955
VpaK11BI GGWCC 1 cut(s) 2020
XapI RAATTY 5 cut(s) 44, 730, 1099, 1462, 2006
XceI RCATGY 4 cut(s) 443, 1016, 1078, 1712
XhoI CTCGAG 1 cut(s) 1537
XmnI GAANNNNTTC 1 cut(s) 1033
XspI CTAG 5 cut(s) 414, 1295, 1373, 1694, 1730
ZraI GACGTC 1 cut(s) 1997
ZrmI AGTACT 1 cut(s) 1575
Zsp2I ATGCAT 3 cut(s) 917, 1014, 1400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.